JPWO2006062189A1 - ニトリルヒドラターゼを発現する形質転換体 - Google Patents

ニトリルヒドラターゼを発現する形質転換体 Download PDF

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Publication number
JPWO2006062189A1
JPWO2006062189A1 JP2006546771A JP2006546771A JPWO2006062189A1 JP WO2006062189 A1 JPWO2006062189 A1 JP WO2006062189A1 JP 2006546771 A JP2006546771 A JP 2006546771A JP 2006546771 A JP2006546771 A JP 2006546771A JP WO2006062189 A1 JPWO2006062189 A1 JP WO2006062189A1
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Japan
Prior art keywords
nitrile hydratase
subunit
activity
amino acid
seq
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Pending
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JP2006546771A
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English (en)
Japanese (ja)
Inventor
加夫留 古家
加夫留 古家
彰 玉木
彰 玉木
伸一郎 長澤
伸一郎 長澤
あやの 鈴木
あやの 鈴木
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Asahi Kasei Corp
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Asahi Kasei Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2006546771A 2004-12-09 2005-12-09 ニトリルヒドラターゼを発現する形質転換体 Pending JPWO2006062189A1 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2004357035 2004-12-09
JP2004357035 2004-12-09
PCT/JP2005/022647 WO2006062189A1 (ja) 2004-12-09 2005-12-09 ニトリルヒドラターゼを発現する形質転換体

Publications (1)

Publication Number Publication Date
JPWO2006062189A1 true JPWO2006062189A1 (ja) 2008-06-12

Family

ID=36578009

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2006546771A Pending JPWO2006062189A1 (ja) 2004-12-09 2005-12-09 ニトリルヒドラターゼを発現する形質転換体

Country Status (4)

Country Link
JP (1) JPWO2006062189A1 (enExample)
KR (1) KR20070085922A (enExample)
TW (1) TW200634151A (enExample)
WO (1) WO2006062189A1 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5080787B2 (ja) * 2006-11-15 2012-11-21 達彦 小林 タンパク質改変方法、ニトリルヒドラターゼ成熟化方法、成熟化ニトリルヒドラターゼ生産方法、成熟化ニトリルヒドラターゼを用いたアミド化合物生産方法
JP5817088B2 (ja) * 2010-03-24 2015-11-18 三菱レイヨン株式会社 ニトリルヒドラターゼ遺伝子を欠失又は不活性化させた微生物
JP2011217665A (ja) * 2010-04-08 2011-11-04 Mitsubishi Rayon Co Ltd ニトリルヒドラターゼ遺伝子を置換した微生物
BR112014020899A2 (pt) 2012-02-28 2018-05-02 Mitsubishi Rayon Co., Ltd. métodos para produzir, preservar, e ativar nitrila hidratase
JP6156442B2 (ja) * 2015-05-28 2017-07-05 三菱ケミカル株式会社 ニトリルヒドラターゼ遺伝子を置換した微生物
EP3564368B1 (en) * 2016-12-28 2025-03-26 Mitsui Chemicals, Inc. Mutant nitrile hydratase, nucleic acid coding said mutant nitrile hydratase, expression vector and transformant including said nucleic acid, production method for said mutant nitrile hydratase, and production method for amide compound
EP3710592B1 (en) 2017-11-14 2022-08-31 Columbia S.r.l. Microbiological process for the preparation of amides
CN111334495B (zh) * 2020-03-12 2022-04-29 宜昌东阳光生化制药有限公司 制备右旋酰胺酮洛芬的方法
CN119220525B (zh) * 2024-09-23 2025-11-21 浙江工业大学 腈水解酶突变体及其在饲料中的应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63137688A (ja) * 1986-12-01 1988-06-09 Res Assoc Util Of Light Oil アミド化合物の製造法
JPH02227069A (ja) * 1989-02-28 1990-09-10 Hideaki Yamada 細菌の培養法
JPH09248188A (ja) * 1996-03-18 1997-09-22 Sumitomo Chem Co Ltd ニトリルヒドラターゼ、その遺伝子及びその利用
WO1999055719A1 (en) * 1998-04-29 1999-11-04 Michigan State University Method for producing amide compounds using a nitrile hydratase from a thermophilic bacillus
KR20010047310A (ko) * 1999-11-19 2001-06-15 이상현 로도코커스 로도크로스(Ｒｈｏｄｏｃｏｃｃｕｓｒｈｏｄｏｃｈｒｏｕｓ) Ｍ33 ＶＫＭ Ａｃ-1515Ｄ로부터유래된 니트릴 히드라타제를 코딩하는 유전자 및 이유전자를 함유한 형질전환체

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2708936B1 (fr) * 1993-08-10 1995-11-10 Rhone Poulenc Chimie Enzymes à activité nitrile-hydratase, outils génétiques et micro-organismes hôtes permettant leur obtention et procédé d'hydrolyse mettant en Óoeuvre lesdites enzymes.
CN1806047A (zh) * 2003-06-10 2006-07-19 旭化成株式会社 新型腈水合酶

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63137688A (ja) * 1986-12-01 1988-06-09 Res Assoc Util Of Light Oil アミド化合物の製造法
JPH02227069A (ja) * 1989-02-28 1990-09-10 Hideaki Yamada 細菌の培養法
JPH09248188A (ja) * 1996-03-18 1997-09-22 Sumitomo Chem Co Ltd ニトリルヒドラターゼ、その遺伝子及びその利用
WO1999055719A1 (en) * 1998-04-29 1999-11-04 Michigan State University Method for producing amide compounds using a nitrile hydratase from a thermophilic bacillus
KR20010047310A (ko) * 1999-11-19 2001-06-15 이상현 로도코커스 로도크로스(Ｒｈｏｄｏｃｏｃｃｕｓｒｈｏｄｏｃｈｒｏｕｓ) Ｍ33 ＶＫＭ Ａｃ-1515Ｄ로부터유래된 니트릴 히드라타제를 코딩하는 유전자 및 이유전자를 함유한 형질전환체

Also Published As

Publication number Publication date
WO2006062189A1 (ja) 2006-06-15
TW200634151A (en) 2006-10-01
TWI327596B (enExample) 2010-07-21
KR20070085922A (ko) 2007-08-27

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