TW200634151A - Transformant expressing nitrile hydratase - Google Patents

Transformant expressing nitrile hydratase

Info

Publication number
TW200634151A
TW200634151A TW094143462A TW94143462A TW200634151A TW 200634151 A TW200634151 A TW 200634151A TW 094143462 A TW094143462 A TW 094143462A TW 94143462 A TW94143462 A TW 94143462A TW 200634151 A TW200634151 A TW 200634151A
Authority
TW
Taiwan
Prior art keywords
nitrile hydratase
transformant expressing
amide compound
expressing nitrile
compound
Prior art date
Application number
TW094143462A
Other languages
Chinese (zh)
Other versions
TWI327596B (en
Inventor
Kaoru Furuya
Akira Tamaki
Shin-Ichiro Nagasawa
Ayano Suzuki
Original Assignee
Asahi Chemical Ind
Tongsuh Petrochemical Corp Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asahi Chemical Ind, Tongsuh Petrochemical Corp Ltd filed Critical Asahi Chemical Ind
Publication of TW200634151A publication Critical patent/TW200634151A/en
Application granted granted Critical
Publication of TWI327596B publication Critical patent/TWI327596B/zh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

It is intended to provide a method of producing an amide compound by using nitrile hydratase which has a high thermal stability and can sustain a high activity even in the presence of a nitrile compound that is the substrate or an amide compound that is a product at a high concentration. A novel microorganism capable of expressing the desired nitrile hydratase is searched for in nature. Then, transformant cells belonging to the genus Rhodococcus, which carry a gene of the above-described enzyme having been transferred thereinto, or processed cells thereof are employed in the production.
TW094143462A 2004-12-09 2005-12-08 Transformant expressing nitrile hydratase TW200634151A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2004357035 2004-12-09

Publications (2)

Publication Number Publication Date
TW200634151A true TW200634151A (en) 2006-10-01
TWI327596B TWI327596B (en) 2010-07-21

Family

ID=36578009

Family Applications (1)

Application Number Title Priority Date Filing Date
TW094143462A TW200634151A (en) 2004-12-09 2005-12-08 Transformant expressing nitrile hydratase

Country Status (4)

Country Link
JP (1) JPWO2006062189A1 (en)
KR (1) KR20070085922A (en)
TW (1) TW200634151A (en)
WO (1) WO2006062189A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111334495A (en) * 2020-03-12 2020-06-26 东莞市东阳光生物合成药有限公司 Method for preparing dexamidoketoprofen

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5080787B2 (en) * 2006-11-15 2012-11-21 達彦 小林 Protein modification method, nitrile hydratase maturation method, mature nitrile hydratase production method, amide compound production method using mature nitrile hydratase
JP5817088B2 (en) * 2010-03-24 2015-11-18 三菱レイヨン株式会社 Microorganisms with deletion or inactivation of the nitrile hydratase gene
JP2011217665A (en) * 2010-04-08 2011-11-04 Mitsubishi Rayon Co Ltd Microorganism in which nitrile hydratase gene is replaced
EP2821483B1 (en) * 2012-02-28 2017-03-29 Mitsubishi Rayon Co., Ltd. Method for preserving enzyme
JP6156442B2 (en) * 2015-05-28 2017-07-05 三菱ケミカル株式会社 Microorganisms that have replaced the nitrile hydratase gene
JP6757422B2 (en) * 2016-12-28 2020-09-16 三井化学株式会社 A mutant nitrile hydratase, a nucleic acid encoding the mutant nitrile hydrase, an expression vector and transformant containing the nucleic acid, a method for producing the mutant nitrile hydrase, and a method for producing an amide compound.
WO2019096677A1 (en) * 2017-11-14 2019-05-23 Columbia Srl Microbiological process for the preparation of amides

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63137688A (en) * 1986-12-01 1988-06-09 Res Assoc Util Of Light Oil Production of amide compound
JPH0753104B2 (en) * 1989-02-28 1995-06-07 秀明 山田 Bacterial culture method
FR2708936B1 (en) * 1993-08-10 1995-11-10 Rhone Poulenc Chimie Nitrile hydratase enzymes, genetic tools and host microorganisms for their production and hydrolysis process using said enzymes.
JP3763157B2 (en) * 1996-03-18 2006-04-05 住友化学株式会社 Nitrile hydratase, its gene and use thereof
US6153415A (en) * 1998-04-29 2000-11-28 Board Of Trustees Operating Michigan State University Method for producing amide compounds using a nitrile hydratase from a thermophilic bacillus
KR100320038B1 (en) * 1999-11-19 2002-02-01 이상현 Gene coding for nitrile hydratase from Rhodococcus rhodochrous M33 VKM Ac-1515D and transformant containing the same
KR100806991B1 (en) * 2003-06-10 2008-02-25 아사히 가세이 가부시키가이샤 Novel nitrile hydratase

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111334495A (en) * 2020-03-12 2020-06-26 东莞市东阳光生物合成药有限公司 Method for preparing dexamidoketoprofen

Also Published As

Publication number Publication date
JPWO2006062189A1 (en) 2008-06-12
WO2006062189A1 (en) 2006-06-15
KR20070085922A (en) 2007-08-27
TWI327596B (en) 2010-07-21

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Legal Events

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MM4A Annulment or lapse of patent due to non-payment of fees