FR2708936B1 - Enzymes à activité nitrile-hydratase, outils génétiques et micro-organismes hôtes permettant leur obtention et procédé d'hydrolyse mettant en Óoeuvre lesdites enzymes. - Google Patents

Enzymes à activité nitrile-hydratase, outils génétiques et micro-organismes hôtes permettant leur obtention et procédé d'hydrolyse mettant en Óoeuvre lesdites enzymes.

Info

Publication number
FR2708936B1
FR2708936B1 FR9309990A FR9309990A FR2708936B1 FR 2708936 B1 FR2708936 B1 FR 2708936B1 FR 9309990 A FR9309990 A FR 9309990A FR 9309990 A FR9309990 A FR 9309990A FR 2708936 B1 FR2708936 B1 FR 2708936B1
Authority
FR
France
Prior art keywords
enzymes
production
nitrile hydratase
hydrolysis process
host microorganisms
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
FR9309990A
Other languages
English (en)
Other versions
FR2708936A1 (fr
Inventor
Cerbelaud Edith
Levy-Schil Sophie
Petre Dominique
Soubrier Fabienne
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rhodia Chimie SAS
Original Assignee
Rhone Poulenc Chimie SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rhone Poulenc Chimie SA filed Critical Rhone Poulenc Chimie SA
Priority to FR9309990A priority Critical patent/FR2708936B1/fr
Priority to EP94925508A priority patent/EP0665889A1/fr
Priority to CA002146656A priority patent/CA2146656A1/fr
Priority to JP7506261A priority patent/JPH08504599A/ja
Priority to PCT/FR1994/000993 priority patent/WO1995004828A1/fr
Publication of FR2708936A1 publication Critical patent/FR2708936A1/fr
Application granted granted Critical
Publication of FR2708936B1 publication Critical patent/FR2708936B1/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
FR9309990A 1993-08-10 1993-08-10 Enzymes à activité nitrile-hydratase, outils génétiques et micro-organismes hôtes permettant leur obtention et procédé d'hydrolyse mettant en Óoeuvre lesdites enzymes. Expired - Fee Related FR2708936B1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
FR9309990A FR2708936B1 (fr) 1993-08-10 1993-08-10 Enzymes à activité nitrile-hydratase, outils génétiques et micro-organismes hôtes permettant leur obtention et procédé d'hydrolyse mettant en Óoeuvre lesdites enzymes.
EP94925508A EP0665889A1 (fr) 1993-08-10 1994-08-09 Enzymes a activite nitrile-hydratase, outils genetiques et micro-organismes hotes permettant leur obtention et procede d'hydrolyse mettant en uvre lesdites enzymes
CA002146656A CA2146656A1 (fr) 1993-08-10 1994-08-09 Enzymes a activite nitrile-hydratase, outils genetiques et micro-organismes hotes permettant leur obtention et procede d'hydrolyse mettant en oeuvre lesdites enzymes
JP7506261A JPH08504599A (ja) 1993-08-10 1994-08-09 ニトリルヒドラターゼ活性を有する酵素、それらの製造のための遺伝学的ツール及び宿主微生物、並びに該酵素を用いる加水分解工程
PCT/FR1994/000993 WO1995004828A1 (fr) 1993-08-10 1994-08-09 Enzymes a activite nitrile-hydratase, outils genetiques et micro-organismes hotes permettant leur obtention et procede d'hydrolyse mettant en ×uvre lesdites enzymes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9309990A FR2708936B1 (fr) 1993-08-10 1993-08-10 Enzymes à activité nitrile-hydratase, outils génétiques et micro-organismes hôtes permettant leur obtention et procédé d'hydrolyse mettant en Óoeuvre lesdites enzymes.

Publications (2)

Publication Number Publication Date
FR2708936A1 FR2708936A1 (fr) 1995-02-17
FR2708936B1 true FR2708936B1 (fr) 1995-11-10

Family

ID=9450218

Family Applications (1)

Application Number Title Priority Date Filing Date
FR9309990A Expired - Fee Related FR2708936B1 (fr) 1993-08-10 1993-08-10 Enzymes à activité nitrile-hydratase, outils génétiques et micro-organismes hôtes permettant leur obtention et procédé d'hydrolyse mettant en Óoeuvre lesdites enzymes.

Country Status (5)

Country Link
EP (1) EP0665889A1 (fr)
JP (1) JPH08504599A (fr)
CA (1) CA2146656A1 (fr)
FR (1) FR2708936B1 (fr)
WO (1) WO1995004828A1 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU7514896A (en) * 1995-10-06 1997-04-28 E.I. Du Pont De Nemours And Company Nucleic acid fragments encoding stereospecific nitrile hydratase and amidase enzymes and recombinant microorganisms expressing those enzymes useful for the production of chiral amides and acides
GB9525372D0 (en) * 1995-12-12 1996-02-14 Allied Colloids Ltd Enzymes, their preparation and their use in the production of ammonium acrylate
US5728556A (en) * 1996-03-14 1998-03-17 E. I. Du Pont De Nemours And Company Production of ω-cyanocarboxamides from aliphatic α,ω-dinitriles using pseudomonas putida-derived biocatalysts
US5866379A (en) * 1997-01-28 1999-02-02 Novus International Enzymatic conversion of α-hydroxynitriles to the corresponding .alpha.
US20040225116A1 (en) * 2003-05-08 2004-11-11 Payne Mark S. Nucleic acid fragments encoding nitrile hydratase and amidase enzymes from comamonas testosteroni 5-MGAM-4D and recombinant organisms expressing those enzymes useful for the production of amides and acids
WO2006049618A1 (fr) * 2004-11-01 2006-05-11 E. I. Du Pont De Nemours And Company Nitrile hydratase et amidase de comamonas testoteroni 5-mgam-4d
TW200634151A (en) * 2004-12-09 2006-10-01 Asahi Chemical Ind Transformant expressing nitrile hydratase
CA2796158C (fr) 2010-04-14 2022-06-14 Strategic Enzyme Applications, Inc. Procede de production de phosphinothricine utilisant des nitrilases
CN116574750B (zh) * 2023-04-21 2023-12-05 大连理工大学 一种提高腈类化合物生物转化效率的腈水合酶重组质粒及其构建方法与应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN85107055A (zh) * 1984-10-01 1986-06-10 诺沃工业联合股票公司 酶促工艺方法
JP2907479B2 (ja) * 1990-02-28 1999-06-21 輝彦 別府 ニトリルヒドラターゼ活性を有するポリペプチドをコードする遺伝子ｄｎａ、これを含有する形質転換体及びアミド類の製造法
EP0502476B1 (fr) * 1991-03-04 2001-07-18 Mitsubishi Rayon Co., Ltd. Vecteurs plasmidiques hybrides contenant des gènes codant pour des enzymes qui dégradent des nitriles et procédés por la préparation d'amides et d'acides

Also Published As

Publication number Publication date
JPH08504599A (ja) 1996-05-21
CA2146656A1 (fr) 1995-02-16
FR2708936A1 (fr) 1995-02-17
EP0665889A1 (fr) 1995-08-09
WO1995004828A1 (fr) 1995-02-16

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