TWI327596B - - Google Patents
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- TWI327596B TWI327596B TW094143462A TW94143462A TWI327596B TW I327596 B TWI327596 B TW I327596B TW 094143462 A TW094143462 A TW 094143462A TW 94143462 A TW94143462 A TW 94143462A TW I327596 B TWI327596 B TW I327596B
- Authority
- TW
- Taiwan
- Prior art keywords
- nitrilase
- sequence
- subunit
- strain
- amino acid
- Prior art date
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- 108010033272 Nitrilase Proteins 0.000 claims description 193
- 108090000623 proteins and genes Proteins 0.000 claims description 84
- 230000000694 effects Effects 0.000 claims description 73
- 108020004414 DNA Proteins 0.000 claims description 69
- -1 hydrazine compound Amine compound Chemical class 0.000 claims description 64
- 244000005700 microbiome Species 0.000 claims description 64
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 47
- 102000004190 Enzymes Human genes 0.000 claims description 44
- 108090000790 Enzymes Proteins 0.000 claims description 44
- 238000004519 manufacturing process Methods 0.000 claims description 32
- 102000004169 proteins and genes Human genes 0.000 claims description 32
- NLHHRLWOUZZQLW-UHFFFAOYSA-N Acrylonitrile Chemical compound C=CC#N NLHHRLWOUZZQLW-UHFFFAOYSA-N 0.000 claims description 29
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 claims description 25
- 241000316848 Rhodococcus <scale insect> Species 0.000 claims description 25
- 241000187693 Rhodococcus rhodochrous Species 0.000 claims description 25
- 230000001580 bacterial effect Effects 0.000 claims description 19
- 150000001413 amino acids Chemical class 0.000 claims description 16
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 15
- 108090000604 Hydrolases Proteins 0.000 claims description 15
- JFDZBHWFFUWGJE-UHFFFAOYSA-N benzonitrile Chemical compound N#CC1=CC=CC=C1 JFDZBHWFFUWGJE-UHFFFAOYSA-N 0.000 claims description 15
- 239000011159 matrix material Substances 0.000 claims description 15
- 241000894006 Bacteria Species 0.000 claims description 14
- 238000001962 electrophoresis Methods 0.000 claims description 14
- 102000004157 Hydrolases Human genes 0.000 claims description 13
- 150000001875 compounds Chemical class 0.000 claims description 12
- 239000007864 aqueous solution Substances 0.000 claims description 11
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 10
- RFFFKMOABOFIDF-UHFFFAOYSA-N Pentanenitrile Chemical compound CCCCC#N RFFFKMOABOFIDF-UHFFFAOYSA-N 0.000 claims description 9
- 230000009466 transformation Effects 0.000 claims description 8
- 239000002253 acid Substances 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 5
- LRDFRRGEGBBSRN-UHFFFAOYSA-N isobutyronitrile Chemical compound CC(C)C#N LRDFRRGEGBBSRN-UHFFFAOYSA-N 0.000 claims description 5
- 239000000758 substrate Substances 0.000 claims description 5
- 108091026890 Coding region Proteins 0.000 claims description 4
- 238000007792 addition Methods 0.000 claims description 4
- BTGRAWJCKBQKAO-UHFFFAOYSA-N adiponitrile Chemical compound N#CCCCCC#N BTGRAWJCKBQKAO-UHFFFAOYSA-N 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 4
- KVNRLNFWIYMESJ-UHFFFAOYSA-N butyronitrile Chemical compound CCCC#N KVNRLNFWIYMESJ-UHFFFAOYSA-N 0.000 claims description 3
- 238000012217 deletion Methods 0.000 claims description 3
- 230000037430 deletion Effects 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 3
- AILKHAQXUAOOFU-UHFFFAOYSA-N hexanenitrile Chemical compound CCCCCC#N AILKHAQXUAOOFU-UHFFFAOYSA-N 0.000 claims description 3
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- 150000007513 acids Chemical group 0.000 claims 1
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- 239000008363 phosphate buffer Substances 0.000 description 8
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 8
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- 101000823746 Acidianus ambivalens Uncharacterized 17.7 kDa protein in bps2 3'region Proteins 0.000 description 7
- 101000916369 Acidianus ambivalens Uncharacterized protein in sor 5'region Proteins 0.000 description 7
- 101000769342 Acinetobacter guillouiae Uncharacterized protein in rpoN-murA intergenic region Proteins 0.000 description 7
- 101000823696 Actinobacillus pleuropneumoniae Uncharacterized glycosyltransferase in aroQ 3'region Proteins 0.000 description 7
- 229920000936 Agarose Polymers 0.000 description 7
- 101000786513 Agrobacterium tumefaciens (strain 15955) Uncharacterized protein outside the virF region Proteins 0.000 description 7
- 101000618005 Alkalihalobacillus pseudofirmus (strain ATCC BAA-2126 / JCM 17055 / OF4) Uncharacterized protein BpOF4_00885 Proteins 0.000 description 7
- 101100437895 Alternaria brassicicola bsc3 gene Proteins 0.000 description 7
- 101000967489 Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / LMG 6465 / NBRC 14845 / NCIMB 13405 / ORS 571) Uncharacterized protein AZC_3924 Proteins 0.000 description 7
- 101000823761 Bacillus licheniformis Uncharacterized 9.4 kDa protein in flaL 3'region Proteins 0.000 description 7
- 101000819719 Bacillus methanolicus Uncharacterized N-acetyltransferase in lysA 3'region Proteins 0.000 description 7
- 101000789586 Bacillus subtilis (strain 168) UPF0702 transmembrane protein YkjA Proteins 0.000 description 7
- 101000792624 Bacillus subtilis (strain 168) Uncharacterized protein YbxH Proteins 0.000 description 7
- 101000790792 Bacillus subtilis (strain 168) Uncharacterized protein YckC Proteins 0.000 description 7
- 101000819705 Bacillus subtilis (strain 168) Uncharacterized protein YlxR Proteins 0.000 description 7
- 101000948218 Bacillus subtilis (strain 168) Uncharacterized protein YtxJ Proteins 0.000 description 7
- 101000718627 Bacillus thuringiensis subsp. kurstaki Putative RNA polymerase sigma-G factor Proteins 0.000 description 7
- 101000641200 Bombyx mori densovirus Putative non-structural protein Proteins 0.000 description 7
- 101000947633 Claviceps purpurea Uncharacterized 13.8 kDa protein Proteins 0.000 description 7
- 101000948901 Enterobacteria phage T4 Uncharacterized 16.0 kDa protein in segB-ipI intergenic region Proteins 0.000 description 7
- 101000805958 Equine herpesvirus 4 (strain 1942) Virion protein US10 homolog Proteins 0.000 description 7
- 101000790442 Escherichia coli Insertion element IS2 uncharacterized 11.1 kDa protein Proteins 0.000 description 7
- 101000788354 Escherichia phage P2 Uncharacterized 8.2 kDa protein in gpA 5'region Proteins 0.000 description 7
- 101000770304 Frankia alni UPF0460 protein in nifX-nifW intergenic region Proteins 0.000 description 7
- 101000797344 Geobacillus stearothermophilus Putative tRNA (cytidine(34)-2'-O)-methyltransferase Proteins 0.000 description 7
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- 101000878851 Leptolyngbya boryana Putative Fe(2+) transport protein A Proteins 0.000 description 7
- 101000758828 Methanosarcina barkeri (strain Fusaro / DSM 804) Uncharacterized protein Mbar_A1602 Proteins 0.000 description 7
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- 101000711023 Rhizobium leguminosarum bv. trifolii Uncharacterized protein in tfuA 3'region Proteins 0.000 description 7
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- 101000917565 Rhodococcus fascians Uncharacterized 33.6 kDa protein in fasciation locus Proteins 0.000 description 7
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2004357035 | 2004-12-09 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| TW200634151A TW200634151A (en) | 2006-10-01 |
| TWI327596B true TWI327596B (enrdf_load_stackoverflow) | 2010-07-21 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| TW094143462A TW200634151A (en) | 2004-12-09 | 2005-12-08 | Transformant expressing nitrile hydratase |
Country Status (4)
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| JP (1) | JPWO2006062189A1 (enrdf_load_stackoverflow) |
| KR (1) | KR20070085922A (enrdf_load_stackoverflow) |
| TW (1) | TW200634151A (enrdf_load_stackoverflow) |
| WO (1) | WO2006062189A1 (enrdf_load_stackoverflow) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5080787B2 (ja) * | 2006-11-15 | 2012-11-21 | 達彦 小林 | タンパク質改変方法、ニトリルヒドラターゼ成熟化方法、成熟化ニトリルヒドラターゼ生産方法、成熟化ニトリルヒドラターゼを用いたアミド化合物生産方法 |
| JP5817088B2 (ja) * | 2010-03-24 | 2015-11-18 | 三菱レイヨン株式会社 | ニトリルヒドラターゼ遺伝子を欠失又は不活性化させた微生物 |
| JP2011217665A (ja) * | 2010-04-08 | 2011-11-04 | Mitsubishi Rayon Co Ltd | ニトリルヒドラターゼ遺伝子を置換した微生物 |
| WO2013129179A1 (ja) * | 2012-02-28 | 2013-09-06 | 三菱レイヨン株式会社 | 酵素の保存方法 |
| JP6156442B2 (ja) * | 2015-05-28 | 2017-07-05 | 三菱ケミカル株式会社 | ニトリルヒドラターゼ遺伝子を置換した微生物 |
| WO2018124247A1 (ja) * | 2016-12-28 | 2018-07-05 | 三井化学株式会社 | 変異型ニトリルヒドラターゼ、該変異型ニトリルヒドラターゼをコードする核酸、該核酸を含む発現ベクター及び形質転換体、該変異型ニトリルヒドラターゼの製造方法、並びにアミド化合物の製造方法 |
| WO2019096677A1 (en) * | 2017-11-14 | 2019-05-23 | Columbia Srl | Microbiological process for the preparation of amides |
| CN111334495B (zh) * | 2020-03-12 | 2022-04-29 | 宜昌东阳光生化制药有限公司 | 制备右旋酰胺酮洛芬的方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63137688A (ja) * | 1986-12-01 | 1988-06-09 | Res Assoc Util Of Light Oil | アミド化合物の製造法 |
| JPH0753104B2 (ja) * | 1989-02-28 | 1995-06-07 | 秀明 山田 | 細菌の培養法 |
| FR2708936B1 (fr) * | 1993-08-10 | 1995-11-10 | Rhone Poulenc Chimie | Enzymes à activité nitrile-hydratase, outils génétiques et micro-organismes hôtes permettant leur obtention et procédé d'hydrolyse mettant en Óoeuvre lesdites enzymes. |
| JP3763157B2 (ja) * | 1996-03-18 | 2006-04-05 | 住友化学株式会社 | ニトリルヒドラターゼ、その遺伝子及びその利用 |
| US6153415A (en) * | 1998-04-29 | 2000-11-28 | Board Of Trustees Operating Michigan State University | Method for producing amide compounds using a nitrile hydratase from a thermophilic bacillus |
| KR100320038B1 (ko) * | 1999-11-19 | 2002-02-01 | 이상현 | 로도코커스 로도크로스(Ｒｈｏｄｏｃｏｃｃｕｓｒｈｏｄｏｃｈｒｏｕｓ) Ｍ33 ＶＫＭ Ａｃ-1515Ｄ로부터유래된 니트릴 히드라타제를 코딩하는 유전자 및 이유전자를 함유한 형질전환체 |
| KR100806991B1 (ko) * | 2003-06-10 | 2008-02-25 | 아사히 가세이 가부시키가이샤 | 신규 니트릴 히드라타아제 |
-
2005
- 2005-12-08 TW TW094143462A patent/TW200634151A/zh not_active IP Right Cessation
- 2005-12-09 JP JP2006546771A patent/JPWO2006062189A1/ja active Pending
- 2005-12-09 WO PCT/JP2005/022647 patent/WO2006062189A1/ja active Application Filing
- 2005-12-09 KR KR1020077012947A patent/KR20070085922A/ko not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| TW200634151A (en) | 2006-10-01 |
| KR20070085922A (ko) | 2007-08-27 |
| WO2006062189A1 (ja) | 2006-06-15 |
| JPWO2006062189A1 (ja) | 2008-06-12 |
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