RU2010108924A - Способ повышения активности аспарагиназы в растворе - Google Patents
Способ повышения активности аспарагиназы в растворе Download PDFInfo
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- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
- C12N9/82—Asparaginase (3.5.1.1)
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- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/06—Enzymes
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/25—Removal of unwanted matter, e.g. deodorisation or detoxification using enzymes
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
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- C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
- C12Y305/01001—Asparaginase (3.5.1.1)
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/20—Treatment of water, waste water, or sewage by degassing, i.e. liberation of dissolved gases
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/28—Treatment of water, waste water, or sewage by sorption
- C02F1/283—Treatment of water, waste water, or sewage by sorption using coal, charred products, or inorganic mixtures containing them
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/44—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis
- C02F1/441—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis by reverse osmosis
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F1/66—Treatment of water, waste water, or sewage by neutralisation; pH adjustment
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/29—Chlorine compounds
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Abstract
1. Способ повышения активности аспарагиназы в растворе, включающий стадии, на которых: ! а) очищают питьевую воду, чтобы получить очищенную воду с содержанием хлора менее чем около 0,5 частей на миллион, и ! 6) смешивают аспарагиназу с упомянутой очищенной водой с получением раствора аспарагиназы. ! 2. Способ по п.1, в котором питьевую воду очищают кислотой. ! 3. Способ по п.1, в котором питьевую воду очищают активированным углем. ! 4. Способ по п.1, в котором упомянутую очищаемую воду очищают на стадии а) с использованием ионообменной смолы. ! 5. Способ по п.1, в котором упомянутую очищаемую воду очищают на стадии а) с использованием метода обратного осмоса. ! 6. Способ по п.1, в котором упомянутую очищаемую воду очищают методом аэрации. ! 7. Способ по п.1, в котором упомянутый раствор аспарагиназы имеет остаточную активность по меньшей мере около 80%, в частности, остаточную активность по меньшей мере около 80% в течение 30 мин после того, как аспарагиназа была добавлена в питьевую воду. ! 8. Способ по п.1, в котором упомянутую очищаемую воду очищают восстановителем, в частности, упомянутый восстановитель содержит одно или несколько веществ, выбранных из группы, включающей: дигидрат дихлорида олова, сульфит натрия, метабисульфит натрия, аскорбиновую кислоту, производные аскорбиновой кислоты, изоаскорбиновую кислоту (эриторбовую кислоту), соли производных аскорбиновой кислоты, железо, цинк, ионы железа и их сочетания. ! 9. Способ по п.1, в котором упомянутая очищенная вода имеет рН менее чем около 8,0. ! 10. Способ по п.1, в котором упомянутую питьевую воду очищают добавкой, достаточной для снижения конечного содержания хлора до более низкого �
Claims (15)
1. Способ повышения активности аспарагиназы в растворе, включающий стадии, на которых:
а) очищают питьевую воду, чтобы получить очищенную воду с содержанием хлора менее чем около 0,5 частей на миллион, и
6) смешивают аспарагиназу с упомянутой очищенной водой с получением раствора аспарагиназы.
2. Способ по п.1, в котором питьевую воду очищают кислотой.
3. Способ по п.1, в котором питьевую воду очищают активированным углем.
4. Способ по п.1, в котором упомянутую очищаемую воду очищают на стадии а) с использованием ионообменной смолы.
5. Способ по п.1, в котором упомянутую очищаемую воду очищают на стадии а) с использованием метода обратного осмоса.
6. Способ по п.1, в котором упомянутую очищаемую воду очищают методом аэрации.
7. Способ по п.1, в котором упомянутый раствор аспарагиназы имеет остаточную активность по меньшей мере около 80%, в частности, остаточную активность по меньшей мере около 80% в течение 30 мин после того, как аспарагиназа была добавлена в питьевую воду.
8. Способ по п.1, в котором упомянутую очищаемую воду очищают восстановителем, в частности, упомянутый восстановитель содержит одно или несколько веществ, выбранных из группы, включающей: дигидрат дихлорида олова, сульфит натрия, метабисульфит натрия, аскорбиновую кислоту, производные аскорбиновой кислоты, изоаскорбиновую кислоту (эриторбовую кислоту), соли производных аскорбиновой кислоты, железо, цинк, ионы железа и их сочетания.
9. Способ по п.1, в котором упомянутая очищенная вода имеет рН менее чем около 8,0.
10. Способ по п.1, в котором упомянутую питьевую воду очищают добавкой, достаточной для снижения конечного содержания хлора до более низкого уровня, чем без использования добавки, в частности, упомянутую очищаемую воду очищают поглотителем хлора, или упомянутая добавка содержит тиосульфат.
11. Способ получения устойчивого раствора фермента в питьевой воде путем инактивирования одного или нескольких снижающих активность компонентов в упомянутой питьевой воде в достаточной степени для того, чтобы обеспечить остаточную активность фермента по меньшей мере около 80% в течение по меньшей мере около 30 мин после добавления фермента в упомянутую питьевую воду.
12. Способ по п.11, в котором снижающий активность компонент содержит хлор, в частности, упомянутый хлор удаляют путем пропускания упомянутой питьевой воды через активированный уголь.
13. Способ по п.11, в котором в упомянутую питьевую воду добавляют EDTA.
14. Система, содержащая:
источник питьевой воды,
источник аспарагиназы,
систему очистки, позволяющую очищать питьевую воду до смешивания с аспарагиназой, и
систему доставки, позволяющую смешивать очищенную питьевую воду и аспарагиназу.
15. Система по п.14, в которой упомянутая система очистки удаляет или инактивирует хлор.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US11/838,153 US8486684B2 (en) | 2007-08-13 | 2007-08-13 | Method for increasing asparaginase activity in a solution |
US11/838,153 | 2007-08-13 |
Publications (2)
Publication Number | Publication Date |
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RU2010108924A true RU2010108924A (ru) | 2011-09-20 |
RU2446706C2 RU2446706C2 (ru) | 2012-04-10 |
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Application Number | Title | Priority Date | Filing Date |
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RU2010108924/13A RU2446706C2 (ru) | 2007-08-13 | 2008-08-12 | Способ повышения активности аспарагиназы в растворе |
Country Status (19)
Country | Link |
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US (1) | US8486684B2 (ru) |
EP (1) | EP2187764B1 (ru) |
JP (1) | JP2010536344A (ru) |
KR (1) | KR20100056503A (ru) |
CN (1) | CN101784199A (ru) |
AR (1) | AR067922A1 (ru) |
AT (1) | ATE527894T1 (ru) |
AU (1) | AU2008286916B2 (ru) |
BR (1) | BRPI0814478A8 (ru) |
CA (1) | CA2697176C (ru) |
CL (1) | CL2008002385A1 (ru) |
CO (1) | CO6260024A2 (ru) |
ES (1) | ES2373075T3 (ru) |
MX (1) | MX2010001756A (ru) |
PL (1) | PL2187764T3 (ru) |
RU (1) | RU2446706C2 (ru) |
TW (1) | TW200918465A (ru) |
WO (1) | WO2009023674A2 (ru) |
ZA (1) | ZA201001067B (ru) |
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US9095145B2 (en) | 2008-09-05 | 2015-08-04 | Frito-Lay North America, Inc. | Method and system for the direct injection of asparaginase into a food process |
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- 2007-08-13 US US11/838,153 patent/US8486684B2/en active Active
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- 2008-08-12 MX MX2010001756A patent/MX2010001756A/es active IP Right Grant
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- 2008-08-12 EP EP08827336A patent/EP2187764B1/en active Active
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- 2008-08-12 CN CN200880103597A patent/CN101784199A/zh active Pending
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US20090047725A1 (en) | 2009-02-19 |
EP2187764B1 (en) | 2011-10-12 |
JP2010536344A (ja) | 2010-12-02 |
AR067922A1 (es) | 2009-10-28 |
EP2187764A2 (en) | 2010-05-26 |
CO6260024A2 (es) | 2011-03-22 |
BRPI0814478A2 (ru) | 2010-02-12 |
RU2446706C2 (ru) | 2012-04-10 |
WO2009023674A4 (en) | 2009-05-28 |
CA2697176C (en) | 2015-12-29 |
BRPI0814478A8 (pt) | 2019-01-29 |
CA2697176A1 (en) | 2009-02-19 |
AU2008286916B2 (en) | 2012-12-20 |
US8486684B2 (en) | 2013-07-16 |
CN101784199A (zh) | 2010-07-21 |
PL2187764T3 (pl) | 2012-03-30 |
MX2010001756A (es) | 2010-08-04 |
WO2009023674A3 (en) | 2009-04-09 |
WO2009023674A2 (en) | 2009-02-19 |
AU2008286916A1 (en) | 2009-02-19 |
ES2373075T3 (es) | 2012-01-31 |
ZA201001067B (en) | 2010-10-27 |
TW200918465A (en) | 2009-05-01 |
KR20100056503A (ko) | 2010-05-27 |
CL2008002385A1 (es) | 2009-05-22 |
ATE527894T1 (de) | 2011-10-15 |
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