NO309041B1 - DNA-sekvens, rekombinant ekspresjonsvektor og vertscelle omfattende DNA-sekvensen, samt fremgangsmÕte for fremstilling av human faktor VIII-derivat - Google Patents
DNA-sekvens, rekombinant ekspresjonsvektor og vertscelle omfattende DNA-sekvensen, samt fremgangsmÕte for fremstilling av human faktor VIII-derivat Download PDFInfo
- Publication number
- NO309041B1 NO309041B1 NO922325A NO922325A NO309041B1 NO 309041 B1 NO309041 B1 NO 309041B1 NO 922325 A NO922325 A NO 922325A NO 922325 A NO922325 A NO 922325A NO 309041 B1 NO309041 B1 NO 309041B1
- Authority
- NO
- Norway
- Prior art keywords
- factor viii
- human factor
- amino acid
- kda
- dna sequence
- Prior art date
Links
- 101000911390 Homo sapiens Coagulation factor VIII Proteins 0.000 title claims abstract description 33
- 229960000900 human factor viii Drugs 0.000 title claims abstract description 31
- 102000057593 human F8 Human genes 0.000 title claims abstract description 30
- 108091028043 Nucleic acid sequence Proteins 0.000 title claims abstract description 17
- 238000000034 method Methods 0.000 title claims abstract description 16
- 239000013604 expression vector Substances 0.000 title claims abstract description 9
- 238000003259 recombinant expression Methods 0.000 title claims abstract description 8
- 210000004027 cell Anatomy 0.000 claims abstract description 40
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 38
- 238000004519 manufacturing process Methods 0.000 claims abstract description 26
- 108020004414 DNA Proteins 0.000 claims abstract description 23
- 125000000539 amino acid group Chemical group 0.000 claims abstract description 14
- 210000004899 c-terminal region Anatomy 0.000 claims abstract description 8
- 229910021645 metal ion Inorganic materials 0.000 claims abstract description 6
- 241001465754 Metazoa Species 0.000 claims abstract description 5
- 230000008569 process Effects 0.000 claims abstract description 3
- 150000001413 amino acids Chemical class 0.000 claims description 35
- 108090000623 proteins and genes Proteins 0.000 claims description 31
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 28
- 229920001184 polypeptide Polymers 0.000 claims description 26
- 238000013518 transcription Methods 0.000 claims description 15
- 230000035897 transcription Effects 0.000 claims description 15
- 239000002609 medium Substances 0.000 claims description 12
- 210000004102 animal cell Anatomy 0.000 claims description 7
- 230000008488 polyadenylation Effects 0.000 claims description 5
- GOJUJUVQIVIZAV-UHFFFAOYSA-N 2-amino-4,6-dichloropyrimidine-5-carbaldehyde Chemical group NC1=NC(Cl)=C(C=O)C(Cl)=N1 GOJUJUVQIVIZAV-UHFFFAOYSA-N 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 230000028327 secretion Effects 0.000 claims description 3
- 108010076504 Protein Sorting Signals Proteins 0.000 claims description 2
- 238000002955 isolation Methods 0.000 claims description 2
- 235000015097 nutrients Nutrition 0.000 claims description 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 108091036060 Linker DNA Proteins 0.000 abstract 1
- 102000001690 Factor VIII Human genes 0.000 description 124
- 108010054218 Factor VIII Proteins 0.000 description 124
- 229960000301 factor viii Drugs 0.000 description 121
- 235000001014 amino acid Nutrition 0.000 description 33
- 229940024606 amino acid Drugs 0.000 description 33
- 239000002299 complementary DNA Substances 0.000 description 28
- 235000018102 proteins Nutrition 0.000 description 22
- 102000004169 proteins and genes Human genes 0.000 description 22
- 239000013598 vector Substances 0.000 description 22
- 230000000694 effects Effects 0.000 description 17
- 239000012141 concentrate Substances 0.000 description 11
- 239000012634 fragment Substances 0.000 description 10
- 238000001727 in vivo Methods 0.000 description 10
- 239000000463 material Substances 0.000 description 9
- 108090000190 Thrombin Proteins 0.000 description 8
- 241000700605 Viruses Species 0.000 description 7
- 238000011534 incubation Methods 0.000 description 7
- 229960004072 thrombin Drugs 0.000 description 7
- 101150074155 DHFR gene Proteins 0.000 description 6
- 210000004978 chinese hamster ovary cell Anatomy 0.000 description 6
- 238000003776 cleavage reaction Methods 0.000 description 6
- 238000010276 construction Methods 0.000 description 6
- RXWNCPJZOCPEPQ-NVWDDTSBSA-N puromycin Chemical compound C1=CC(OC)=CC=C1C[C@H](N)C(=O)N[C@H]1[C@@H](O)[C@H](N2C3=NC=NC(=C3N=C2)N(C)C)O[C@@H]1CO RXWNCPJZOCPEPQ-NVWDDTSBSA-N 0.000 description 6
- 230000007017 scission Effects 0.000 description 6
- 239000000758 substrate Substances 0.000 description 6
- FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 5
- 108010022394 Threonine synthase Proteins 0.000 description 5
- 238000004113 cell culture Methods 0.000 description 5
- 238000011161 development Methods 0.000 description 5
- 102000004419 dihydrofolate reductase Human genes 0.000 description 5
- 229960000485 methotrexate Drugs 0.000 description 5
- 238000013519 translation Methods 0.000 description 5
- 102000053602 DNA Human genes 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
- 208000009292 Hemophilia A Diseases 0.000 description 4
- 241000829100 Macaca mulatta polyomavirus 1 Species 0.000 description 4
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 4
- 239000000427 antigen Substances 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000012217 deletion Methods 0.000 description 4
- 230000037430 deletion Effects 0.000 description 4
- 239000003623 enhancer Substances 0.000 description 4
- 229940088598 enzyme Drugs 0.000 description 4
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 4
- 239000003550 marker Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- 102100026735 Coagulation factor VIII Human genes 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 3
- 201000003542 Factor VIII deficiency Diseases 0.000 description 3
- 241000701024 Human betaherpesvirus 5 Species 0.000 description 3
- 241000283973 Oryctolagus cuniculus Species 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 230000004071 biological effect Effects 0.000 description 3
- 239000012894 fetal calf serum Substances 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 230000002779 inactivation Effects 0.000 description 3
- 239000002773 nucleotide Substances 0.000 description 3
- 125000003729 nucleotide group Chemical group 0.000 description 3
- 239000000825 pharmaceutical preparation Substances 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 230000006337 proteolytic cleavage Effects 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 229950010131 puromycin Drugs 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 108010047303 von Willebrand Factor Proteins 0.000 description 3
- 102100036537 von Willebrand factor Human genes 0.000 description 3
- 229960001134 von willebrand factor Drugs 0.000 description 3
- 238000001262 western blot Methods 0.000 description 3
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 102000012410 DNA Ligases Human genes 0.000 description 2
- 108010061982 DNA Ligases Proteins 0.000 description 2
- 102000004594 DNA Polymerase I Human genes 0.000 description 2
- 108010017826 DNA Polymerase I Proteins 0.000 description 2
- 230000004544 DNA amplification Effects 0.000 description 2
- 239000004471 Glycine Substances 0.000 description 2
- 101000782195 Homo sapiens von Willebrand factor Proteins 0.000 description 2
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 2
- 241000713333 Mouse mammary tumor virus Species 0.000 description 2
- 101000966481 Mus musculus Dihydrofolate reductase Proteins 0.000 description 2
- 229920002684 Sepharose Polymers 0.000 description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- 108700029229 Transcriptional Regulatory Elements Proteins 0.000 description 2
- 210000001766 X chromosome Anatomy 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 2
- 239000013599 cloning vector Substances 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 210000003527 eukaryotic cell Anatomy 0.000 description 2
- 239000012737 fresh medium Substances 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 208000031169 hemorrhagic disease Diseases 0.000 description 2
- 229940034998 human von willebrand factor Drugs 0.000 description 2
- 238000003119 immunoblot Methods 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 238000004114 suspension culture Methods 0.000 description 2
- 229940104230 thymidine Drugs 0.000 description 2
- 230000007704 transition Effects 0.000 description 2
- 230000014599 transmission of virus Effects 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- DIGQNXIGRZPYDK-WKSCXVIASA-N (2R)-6-amino-2-[[2-[[(2S)-2-[[2-[[(2R)-2-[[(2S)-2-[[(2R,3S)-2-[[2-[[(2S)-2-[[2-[[(2S)-2-[[(2S)-2-[[(2R)-2-[[(2S,3S)-2-[[(2R)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-[[(2R)-2-[[2-[[2-[[2-[(2-amino-1-hydroxyethylidene)amino]-3-carboxy-1-hydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1,5-dihydroxy-5-iminopentylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]hexanoic acid Chemical compound C[C@@H]([C@@H](C(=N[C@@H](CS)C(=N[C@@H](C)C(=N[C@@H](CO)C(=NCC(=N[C@@H](CCC(=N)O)C(=NC(CS)C(=N[C@H]([C@H](C)O)C(=N[C@H](CS)C(=N[C@H](CO)C(=NCC(=N[C@H](CS)C(=NCC(=N[C@H](CCCCN)C(=O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)N=C([C@H](CS)N=C([C@H](CO)N=C([C@H](CO)N=C([C@H](C)N=C(CN=C([C@H](CO)N=C([C@H](CS)N=C(CN=C(C(CS)N=C(C(CC(=O)O)N=C(CN)O)O)O)O)O)O)O)O)O)O)O)O DIGQNXIGRZPYDK-WKSCXVIASA-N 0.000 description 1
- 208000030507 AIDS Diseases 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- 108010087504 Beta-Globulins Proteins 0.000 description 1
- 241000701822 Bovine papillomavirus Species 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 241000282693 Cercopithecidae Species 0.000 description 1
- 108020004638 Circular DNA Proteins 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 241000699800 Cricetinae Species 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 108700041152 Endoplasmic Reticulum Chaperone BiP Proteins 0.000 description 1
- 102100021451 Endoplasmic reticulum chaperone BiP Human genes 0.000 description 1
- 101150112743 HSPA5 gene Proteins 0.000 description 1
- 206010062713 Haemorrhagic diathesis Diseases 0.000 description 1
- 208000031220 Hemophilia Diseases 0.000 description 1
- 108010025815 Kanamycin Kinase Proteins 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- 102000003792 Metallothionein Human genes 0.000 description 1
- 108090000157 Metallothionein Proteins 0.000 description 1
- 102000010722 N-Glycosyl Hydrolases Human genes 0.000 description 1
- 108010063372 N-Glycosyl Hydrolases Proteins 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 241000276498 Pollachius virens Species 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 241000714474 Rous sarcoma virus Species 0.000 description 1
- 101100111629 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) KAR2 gene Proteins 0.000 description 1
- 208000026552 Severe hemophilia A Diseases 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 241000700618 Vaccinia virus Species 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 description 1
- 102000005421 acetyltransferase Human genes 0.000 description 1
- 108020002494 acetyltransferase Proteins 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 102000006646 aminoglycoside phosphotransferase Human genes 0.000 description 1
- 239000012062 aqueous buffer Substances 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 239000013602 bacteriophage vector Substances 0.000 description 1
- 238000002306 biochemical method Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 229910010293 ceramic material Inorganic materials 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000001143 conditioned effect Effects 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 231100000433 cytotoxic Toxicity 0.000 description 1
- 230000001472 cytotoxic effect Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000003795 desorption Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229940042399 direct acting antivirals protease inhibitors Drugs 0.000 description 1
- 230000006334 disulfide bridging Effects 0.000 description 1
- 231100000673 dose–response relationship Toxicity 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 108010052305 exodeoxyribonuclease III Proteins 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000013595 glycosylation Effects 0.000 description 1
- 238000006206 glycosylation reaction Methods 0.000 description 1
- 101150028578 grp78 gene Proteins 0.000 description 1
- 230000002439 hemostatic effect Effects 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- 239000012510 hollow fiber Substances 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 210000004897 n-terminal region Anatomy 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 229940079938 nitrocellulose Drugs 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 101800002712 p27 Proteins 0.000 description 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 230000004481 post-translational protein modification Effects 0.000 description 1
- 230000001323 posttranslational effect Effects 0.000 description 1
- 239000013014 purified material Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 238000001542 size-exclusion chromatography Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 229960003766 thrombin (human) Drugs 0.000 description 1
- 230000005030 transcription termination Effects 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 241000701161 unidentified adenovirus Species 0.000 description 1
- 239000011800 void material Substances 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
- C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Animal Behavior & Ethology (AREA)
- Diabetes (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Compositions Of Oxide Ceramics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- General Preparation And Processing Of Foods (AREA)
- Confectionery (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE8904239A SE465222C5 (sv) | 1989-12-15 | 1989-12-15 | Ett rekombinant, humant faktor VIII-derivat och förfarande för dess framställning |
| PCT/SE1990/000809 WO1991009122A1 (fr) | 1989-12-15 | 1990-12-06 | Derive du facteur humain viii de recombinaison |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| NO922325D0 NO922325D0 (no) | 1992-06-12 |
| NO922325L NO922325L (no) | 1992-08-11 |
| NO309041B1 true NO309041B1 (no) | 2000-12-04 |
Family
ID=20377785
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO922325A NO309041B1 (no) | 1989-12-15 | 1992-06-12 | DNA-sekvens, rekombinant ekspresjonsvektor og vertscelle omfattende DNA-sekvensen, samt fremgangsmÕte for fremstilling av human faktor VIII-derivat |
Country Status (21)
| Country | Link |
|---|---|
| EP (3) | EP1293513A3 (fr) |
| JP (1) | JP2644084B2 (fr) |
| AT (2) | ATE232213T1 (fr) |
| AU (1) | AU645539B2 (fr) |
| BR (1) | BR9007921A (fr) |
| CA (1) | CA2071875C (fr) |
| DE (3) | DE19975066I2 (fr) |
| DK (2) | DK0786474T3 (fr) |
| ES (2) | ES2189897T3 (fr) |
| FI (1) | FI104260B1 (fr) |
| HK (2) | HK1009290A1 (fr) |
| HU (1) | HU211504A9 (fr) |
| IE (1) | IE904510A1 (fr) |
| LU (1) | LU90457I2 (fr) |
| NL (1) | NL990035I2 (fr) |
| NO (1) | NO309041B1 (fr) |
| NZ (1) | NZ236276A (fr) |
| PT (1) | PT96208B (fr) |
| SE (1) | SE465222C5 (fr) |
| SG (1) | SG66753A1 (fr) |
| WO (1) | WO1991009122A1 (fr) |
Families Citing this family (139)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5595886A (en) | 1986-01-27 | 1997-01-21 | Chiron Corporation | Protein complexes having Factor VIII:C activity and production thereof |
| US6060447A (en) * | 1987-05-19 | 2000-05-09 | Chiron Corporation | Protein complexes having Factor VIII:C activity and production thereof |
| IL86693A (en) | 1987-06-12 | 1994-06-24 | Stichting Centraal Lab | Proteins that have the activity IIIV of the blood, a process for their preparation that uses cells are produced through genetic engineering and pharmaceutical preparations that contain them |
| US6346513B1 (en) | 1987-06-12 | 2002-02-12 | Baxter Trading Gmbh | Proteins with factor VIII activity: process for their preparation using genetically-engineered cells and pharmaceutical compositions containing them |
| US5629287A (en) * | 1991-01-18 | 1997-05-13 | University College London | Depot formulations |
| US5610148A (en) * | 1991-01-18 | 1997-03-11 | University College London | Macroscopically oriented cell adhesion protein for wound treatment |
| SE468050C (sv) * | 1991-03-15 | 1998-04-27 | Pharmacia & Upjohn Ab | Rekombinant derivat av human faktor VIII |
| US5663060A (en) * | 1992-04-07 | 1997-09-02 | Emory University | Hybrid human/animal factor VIII |
| US5744446A (en) * | 1992-04-07 | 1998-04-28 | Emory University | Hybrid human/animal factor VIII |
| US5364771A (en) * | 1992-04-07 | 1994-11-15 | Emory University | Hybrid human/porcine factor VIII |
| US5888974A (en) * | 1992-04-07 | 1999-03-30 | Emory University | Hybrid human/animal factor VIII |
| DK53792D0 (da) * | 1992-04-24 | 1992-04-24 | Novo Nordisk As | Fremgangsmaade til fremstilling af proteiner |
| WO1994007510A1 (fr) * | 1992-10-02 | 1994-04-14 | Kabi Pharmacia Ab | Composition comprenant une formulation d'un facteur de coagulation viii, procede pour sa preparation et utilisation d'un agent tensioactif comme stabilisateur |
| SE9300509D0 (sv) * | 1993-02-16 | 1993-02-16 | Kabi Pharmacia Ab | Peg treatment |
| SE9301581D0 (sv) * | 1993-05-07 | 1993-05-07 | Kabi Pharmacia Ab | Protein formulation |
| SE504074C2 (sv) * | 1993-07-05 | 1996-11-04 | Pharmacia Ab | Proteinberedning för subkutan, intramuskulär eller intradermal administrering |
| SE9303601D0 (sv) * | 1993-11-01 | 1993-11-01 | Kabi Pharmacia Ab | Improved cell cultivation method and medium |
| US6288030B1 (en) | 1993-12-22 | 2001-09-11 | Amgen Inc. | Stem cell factor formulations and methods |
| IL113010A0 (en) * | 1994-03-31 | 1995-10-31 | Pharmacia Ab | Pharmaceutical formulation comprising factor VIII or factor ix with an activity of at least 200 IU/ml and an enhancer for improved subcutaneous intramuscular or intradermal administration |
| SE9403915D0 (sv) * | 1994-11-14 | 1994-11-14 | Annelie Almstedt | Process A |
| SE503424C2 (sv) * | 1994-11-14 | 1996-06-10 | Pharmacia Ab | Process för rening av rekombinant koagulationsfaktor VIII |
| US6818439B1 (en) | 1994-12-30 | 2004-11-16 | Chiron Corporation | Methods for administration of recombinant gene delivery vehicles for treatment of hemophilia and other disorders |
| SE9501189D0 (sv) * | 1995-03-31 | 1995-03-31 | Pharmacia Ab | Protein formulation |
| AU6455896A (en) * | 1995-07-11 | 1997-02-10 | Chiron Corporation | Novel factor viii:c polypeptide analogs with altered metal-binding properties |
| SE9503380D0 (sv) * | 1995-09-29 | 1995-09-29 | Pharmacia Ab | Protein derivatives |
| US5851800A (en) * | 1996-05-14 | 1998-12-22 | Pharmacia & Upjohn Ab | Process for producing a protein |
| EP1318198B1 (fr) * | 1996-05-14 | 2006-03-22 | Biovitrum Ab | Procédé pour produire un polypeptide recombine comprenant l'addition d'un inhibiteur de chymotrypsines au milieu de culture cellulaire |
| US6475725B1 (en) | 1997-06-20 | 2002-11-05 | Baxter Aktiengesellschaft | Recombinant cell clones having increased stability and methods of making and using the same |
| JP2002517180A (ja) * | 1997-12-05 | 2002-06-18 | ジ・イミユーン・リスポンス・コーポレーシヨン | 増大された発現を示す新規ベクターおよび遺伝子 |
| US7820796B2 (en) | 1998-03-12 | 2010-10-26 | Genetics Institute, Llc. | Methods for producing Factor VIII proteins |
| US6797505B2 (en) * | 1998-05-27 | 2004-09-28 | Cell Genesys, Inc. | Recombinant AAV vectors for gene therapy of hemophilia A |
| US6200560B1 (en) * | 1998-10-20 | 2001-03-13 | Avigen, Inc. | Adeno-associated virus vectors for expression of factor VIII by target cells |
| US6221349B1 (en) | 1998-10-20 | 2001-04-24 | Avigen, Inc. | Adeno-associated vectors for expression of factor VIII by target cells |
| US6358703B1 (en) | 1998-12-10 | 2002-03-19 | Bayer Corporation | Expression system for factor VIII |
| JP5149470B2 (ja) | 1999-02-22 | 2013-02-20 | バクスター・インターナショナル・インコーポレイテッド | 新規のアルブミンを含有していない第ｖｉｉｉ因子処方物 |
| SE9901379D0 (sv) | 1999-04-19 | 1999-04-19 | Pharmacia & Upjohn Ab | Receptor structures |
| AT409379B (de) | 1999-06-02 | 2002-07-25 | Baxter Ag | Medium zur protein- und serumfreien kultivierung von zellen |
| MXPA02009221A (es) * | 2000-03-22 | 2005-07-25 | Octagene Gmbh | Produccion de factores de coagulacion sanguineo recombinantes en lineas celular humanas. |
| AU2004236174B2 (en) | 2001-10-10 | 2011-06-02 | Novo Nordisk A/S | Glycopegylation methods and proteins/peptides produced by the methods |
| GB0207092D0 (en) | 2002-03-26 | 2002-05-08 | Sod Conseils Rech Applic | Stable pharmaceutical composition containing factor VIII |
| EP1520008B1 (fr) | 2002-07-09 | 2012-09-05 | Baxter International Inc. | Milieu exempt de proteines animales pour la culture de cellules |
| LT1596887T (lt) | 2003-02-26 | 2022-04-25 | Nektar Therapeutics | Polimero-faktoriaus viii fragmento konjugatai |
| TWI353991B (en) | 2003-05-06 | 2011-12-11 | Syntonix Pharmaceuticals Inc | Immunoglobulin chimeric monomer-dimer hybrids |
| US9005625B2 (en) | 2003-07-25 | 2015-04-14 | Novo Nordisk A/S | Antibody toxin conjugates |
| ES2648241T3 (es) | 2003-09-30 | 2017-12-29 | The Trustees Of The University Of Pennsylvania | Clados de virus adenoasociados (AAV), secuencias, vectores que contienen el mismo, y usos de los mismos |
| US20080305992A1 (en) | 2003-11-24 | 2008-12-11 | Neose Technologies, Inc. | Glycopegylated erythropoietin |
| DK2586456T3 (en) | 2004-10-29 | 2016-03-21 | Ratiopharm Gmbh | Conversion and glycopegylation of fibroblast growth factor (FGF) |
| US20060094104A1 (en) | 2004-10-29 | 2006-05-04 | Leopold Grillberger | Animal protein-free media for cultivation of cells |
| MX350293B (es) | 2004-11-12 | 2017-09-04 | Bayer Healthcare Llc | Modificacion dirigida al sitio del factor viii. |
| EP1858543B1 (fr) | 2005-01-10 | 2013-11-27 | BioGeneriX AG | Facteur de stimulation de colonie de granulocytes glycopegylé |
| EP1707634A1 (fr) | 2005-03-29 | 2006-10-04 | Octapharma AG | Procédé pour l'isolation de protéines recombinantes |
| US8999678B2 (en) | 2005-04-07 | 2015-04-07 | The Trustees Of The University Of Pennsylvania | Method of increasing the function of an AAV vector |
| EP2386571B1 (fr) | 2005-04-08 | 2016-06-01 | ratiopharm GmbH | Compositions et méthodes utilisées pour la préparation de mutants par glycosylation de l'hormone de croissance humaine résistant à la protéase |
| EP1739179A1 (fr) | 2005-06-30 | 2007-01-03 | Octapharma AG | Transfection stable exempt de sérum et production des protéines recombinantes humaines dans des lignées cellulaires humaines |
| US20070105755A1 (en) | 2005-10-26 | 2007-05-10 | Neose Technologies, Inc. | One pot desialylation and glycopegylation of therapeutic peptides |
| US20090048440A1 (en) | 2005-11-03 | 2009-02-19 | Neose Technologies, Inc. | Nucleotide Sugar Purification Using Membranes |
| PL3121266T3 (pl) | 2006-01-04 | 2020-07-13 | Baxalta Incorporated | Wolne od oligopeptydów pożywki do hodowli komórkowej |
| US7982010B2 (en) | 2006-03-31 | 2011-07-19 | Baxter International Inc. | Factor VIII polymer conjugates |
| US7645860B2 (en) | 2006-03-31 | 2010-01-12 | Baxter Healthcare S.A. | Factor VIII polymer conjugates |
| US7985839B2 (en) | 2006-03-31 | 2011-07-26 | Baxter International Inc. | Factor VIII polymer conjugates |
| KR20080108147A (ko) | 2006-03-31 | 2008-12-11 | 백스터 인터내셔널 인코포레이티드 | 페질화된 인자 ｖｉｉｉ |
| EP2049144B8 (fr) | 2006-07-21 | 2015-02-18 | ratiopharm GmbH | Glycosylation de peptides par l'intermédiaire de séquences de glycosylation à liaison o |
| US20100075375A1 (en) | 2006-10-03 | 2010-03-25 | Novo Nordisk A/S | Methods for the purification of polypeptide conjugates |
| ES2521490T3 (es) | 2006-12-15 | 2014-11-12 | Baxter International Inc. | Conjugado de factor VIIa - ácido (poli)siálico con una vida media in vivo prolongada. |
| CN101646779B (zh) | 2007-02-23 | 2014-12-03 | Sk化学株式会社 | 制备和纯化因子viii及其衍生物的方法 |
| CN101796063B (zh) | 2007-04-03 | 2017-03-22 | 拉蒂奥法姆有限责任公司 | 使用糖聚乙二醇化g‑csf的治疗方法 |
| ES2551123T3 (es) | 2007-06-12 | 2015-11-16 | Ratiopharm Gmbh | Proceso mejorado para la producción de azúcares de nucleótido |
| US9359629B2 (en) | 2007-12-27 | 2016-06-07 | Baxalta Incorporated | Cell culture processes |
| MX2010009154A (es) | 2008-02-27 | 2010-09-09 | Novo Nordisk As | Moleculas conjugadas del factor viii. |
| LT2393828T (lt) | 2009-02-03 | 2017-01-25 | Amunix Operating Inc. | Prailginti rekombinantiniai polipeptidai ir juos apimančios kompozicijos |
| ES2966234T3 (es) | 2009-06-09 | 2024-04-18 | Prolong Pharmaceuticals Llc | Composiciones de hemoglobina |
| US8809501B2 (en) | 2009-07-27 | 2014-08-19 | Baxter International Inc. | Nucleophilic catalysts for oxime linkage |
| US8642737B2 (en) | 2010-07-26 | 2014-02-04 | Baxter International Inc. | Nucleophilic catalysts for oxime linkage |
| KR101832937B1 (ko) | 2009-07-27 | 2018-02-28 | 박스알타 인코퍼레이티드 | 혈액 응고 단백질 복합체 |
| SG10201401194VA (en) | 2009-07-27 | 2014-07-30 | Lipoxen Technologies Ltd | Glycopolysialylation of non-blood coagulation proteins |
| ES2590679T3 (es) | 2009-07-27 | 2016-11-23 | Lipoxen Technologies Limited | Glicopolisialilación de proteínas diferentes a proteínas de coagulación de la sangre |
| EP2470559B1 (fr) | 2009-08-24 | 2017-03-22 | Amunix Operating Inc. | Compositions de facteur ix de coagulation et leurs procédés de fabrication et d'utilisation |
| GB0915481D0 (en) | 2009-09-04 | 2009-10-07 | Arecor Ltd | Stable manufacture of factor V111 |
| MX336830B (es) | 2009-12-06 | 2016-02-03 | Biogen Hemophilia Inc | Polipeptidos hibridos y quimericos del factor viii-fc, y metodos de uso de los mismos. |
| WO2011095604A1 (fr) | 2010-02-04 | 2011-08-11 | Octapharma Biopharmaceuticals Gmbh | Prolongation de la demi-vie de protéines |
| US9315825B2 (en) | 2010-03-29 | 2016-04-19 | The Trustees Of The University Of Pennsylvania | Pharmacologically induced transgene ablation system |
| CA2793633A1 (fr) | 2010-03-29 | 2011-10-13 | The Trustees Of The University Of Pennsylvania | Systeme d'ablation de transgene induit pharmacologiquement |
| WO2012006635A1 (fr) | 2010-07-09 | 2012-01-12 | Biogen Idec Hemophilia Inc. | Molécules à chaîne unique pouvant être traitées et polypeptides faits à partir de celles-ci |
| WO2012006623A1 (fr) | 2010-07-09 | 2012-01-12 | Biogen Idec Hemophilia Inc. | Systèmes pour le traitement du facteur viii et procédés associés |
| EP2616486B1 (fr) | 2010-09-15 | 2019-01-02 | Stichting Sanquin Bloedvoorziening | Variants du facteur viii à absorption cellulaire réduite |
| EA032056B1 (ru) | 2010-12-22 | 2019-04-30 | Баксалта Инкорпорейтид | Конъюгат терапевтического белка и производного жирной кислоты, способы получения конъюгата терапевтического белка и производного жирной кислоты (варианты) |
| HUE043921T2 (hu) | 2011-02-17 | 2019-09-30 | Univ Pennsylvania | Készítmények és módszerek szövetspecifitás módosítására és AAV9-mediált géntranszfer javítására |
| WO2012170969A2 (fr) | 2011-06-10 | 2012-12-13 | Biogen Idec Ma Inc. | Composés pro-coagulants et leurs procédés d'utilisation |
| EA029045B1 (ru) | 2011-07-08 | 2018-02-28 | Байоджен Хемофилия Инк. | Химерные и гибридные полипептиды фактора viii и способы их применения |
| WO2013016454A1 (fr) | 2011-07-25 | 2013-01-31 | Biogen Idec Hemophilia Inc. | Dosages pour la surveillance de troubles hémostatiques |
| AU2012324020A1 (en) | 2011-12-30 | 2013-07-18 | Grifols, S.A. | Method for purifying Factor VIII |
| LT2802668T (lt) | 2012-01-12 | 2018-11-26 | Bioverativ Therapeutics Inc. | Imunogeninio atsako prieš faktorių viii individuose, kuriems skiriama faktoriaus viii terapija, sumažinimas |
| LT2804623T (lt) | 2012-01-12 | 2019-12-10 | Bioverativ Therapeutics Inc | Chimeriniai viii faktoriaus polipeptidai ir jų panaudojimas |
| RS63870B1 (sr) | 2012-02-15 | 2023-01-31 | Bioverativ Therapeutics Inc | Sastavi faktora viii i postupci za pravljenje i upotrebu istih |
| WO2013123457A1 (fr) | 2012-02-15 | 2013-08-22 | Biogen Idec Ma Inc. | Protéines de facteur viii de recombinaison |
| CN104519897A (zh) | 2012-06-08 | 2015-04-15 | 比奥根艾迪克Ma公司 | 促凝血化合物 |
| US10202595B2 (en) | 2012-06-08 | 2019-02-12 | Bioverativ Therapeutics Inc. | Chimeric clotting factors |
| EP3404105A1 (fr) | 2012-07-06 | 2018-11-21 | Bioverativ Therapeutics Inc. | Lignée cellulaire exprimant des polypeptides du facteur viii à chaîne unique et ses utilisations |
| ES2770501T3 (es) | 2012-07-11 | 2020-07-01 | Bioverativ Therapeutics Inc | Complejo del factor VIII con XTEN y proteína del factor de Von Willebrand y sus usos |
| RU2500818C1 (ru) * | 2012-07-19 | 2013-12-10 | Общество с ограниченной ответственностью "Международный биотехнологический центр "Генериум" ("МБЦ "Генериум") | РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК рАР227, КОДИРУЮЩАЯ ПОЛИПЕПТИД РЕКОМБИНАНТНОГО ФАКТОРА VIII СВЕРТЫВАЕМОСТИ КРОВИ ЧЕЛОВЕКА, ЛИНИЯ КЛЕТОК Cricetulus griseus CHO 2H5 - ПРОДУЦЕНТ РЕКОМБИНАНТНОГО ФАКТОРА VIII СВЕРТЫВАЕМОСТИ КРОВИ ЧЕЛОВЕКА И СПОСОБ ПОЛУЧЕНИЯ ПОЛИПЕПТИДА, ОБЛАДАЮЩЕГО АКТИВНОСТЬЮ ФАКТОРА VIII |
| US10001495B2 (en) | 2012-07-25 | 2018-06-19 | Bioverativ Therapeutics Inc. | Blood factor monitoring assay and uses thereof |
| US10391152B2 (en) | 2012-10-18 | 2019-08-27 | Bioverativ Therapeutics Inc. | Methods of using a fixed dose of a clotting factor |
| US20150266944A1 (en) | 2012-10-30 | 2015-09-24 | Biogen Idec Ma Inc. | Methods of Using FVIII Polypeptide |
| HRP20231183T1 (hr) | 2013-02-15 | 2024-01-05 | Bioverativ Therapeutics Inc. | Optimizirani gen faktora viii |
| TWI683666B (zh) | 2013-03-15 | 2020-02-01 | 美商百歐維拉提夫治療公司 | 因子ｉｘ多肽調配物 |
| JP6330026B2 (ja) | 2013-03-15 | 2018-05-23 | バイオベラティブ セラピューティクス インコーポレイテッド | 第ｖｉｉｉ因子ポリペプチド製剤 |
| WO2015012924A2 (fr) | 2013-04-29 | 2015-01-29 | The Trustees Of The University Of Pennsylvania | Cassettes d'expression préférentielle pour tissus modifiées par un codon, vecteurs les contenant, et utilisation |
| EP4368194A3 (fr) | 2013-06-28 | 2024-07-31 | Bioverativ Therapeutics Inc. | Lieur clivable par thrombine ayant xten et ses utilisations |
| EP3875106A1 (fr) | 2013-08-08 | 2021-09-08 | Bioverativ Therapeutics Inc. | Purification des molécules fviii chimériques |
| US20160311885A1 (en) | 2013-08-14 | 2016-10-27 | Biogen Ma Inc. | Recombinant factor viii proteins |
| TWI667255B (zh) | 2013-08-14 | 2019-08-01 | 美商生物化學醫療公司 | 因子ｖｉｉｉ－ｘｔｅｎ融合物及其用途 |
| WO2015048330A2 (fr) | 2013-09-25 | 2015-04-02 | Biogen Idec Ma Inc. | Procédés d'inactivation de virus sur colonne |
| EP3060242A1 (fr) | 2013-10-22 | 2016-08-31 | DBV Technologies | Procédé pour traiter l'hémophilie en induisant une tolérance de facteurs sanguins |
| EP3065769A4 (fr) | 2013-11-08 | 2017-05-31 | Biogen MA Inc. | Composé de fusion procoagulant |
| WO2015085276A1 (fr) | 2013-12-06 | 2015-06-11 | Biogen Idec Ma Inc. | Outils pharmacocinétiques de population et leurs utilisations |
| EP3757116A1 (fr) | 2013-12-09 | 2020-12-30 | Sangamo Therapeutics, Inc. | Procédés et compositions pour l'ingénierie génomique |
| SG11201605242YA (en) | 2014-01-10 | 2016-07-28 | Biogen Ma Inc | Factor viii chimeric proteins and uses thereof |
| AU2015214245B2 (en) | 2014-02-04 | 2020-09-10 | Biogen Ma Inc. | Use of cation-exchange chromatography in the flow-through mode to enrich post-translational modifications |
| WO2016012595A1 (fr) * | 2014-07-25 | 2016-01-28 | Csl Behring Gmbh | Préparations améliorées de facteur viii appropriés pour une utilisation thérapeutique et leurs procédés d'obtention |
| MA40864A (fr) | 2014-10-31 | 2017-09-05 | Biogen Ma Inc | Hypotaurine, gaba, bêta-alanine et choline pour la régulation de l'accumulation de sous-produits résiduaires dans des procédés de culture de cellules mammifères |
| WO2016168728A2 (fr) | 2015-04-16 | 2016-10-20 | Emory University | Promoteurs et vecteurs recombinés pour l'expression de protéines dans le foie et utilisation associée |
| UA126016C2 (uk) | 2015-08-03 | 2022-08-03 | Біовератів Терапеутікс Інк. | Злитий білок фактора іх |
| EP3411478B1 (fr) | 2016-02-01 | 2022-06-08 | Bioverativ Therapeutics Inc. | Gènes du facteur viii optimisés |
| US20200085915A1 (en) | 2016-12-02 | 2020-03-19 | Bioverativ Therapeutics Inc. | Methods of inducing immune tolerance to clotting factors |
| EP3548066A1 (fr) | 2016-12-02 | 2019-10-09 | Bioverativ Therapeutics Inc. | Méthodes de traitement de l'arthropathie hémophilique à l'aide de facteurs de coagulation chimériques |
| KR20200035130A (ko) | 2017-08-09 | 2020-04-01 | 바이오버라티브 테라퓨틱스 인크. | 핵산 분자 및 이의 용도 |
| WO2019067766A1 (fr) | 2017-09-27 | 2019-04-04 | Sigilon Therapeutics, Inc. | Procédés, compositions et éléments implantables comprenant des cellules actives |
| CA3090136A1 (fr) | 2018-02-01 | 2019-08-08 | Bioverativ Therapeutics, Inc. | Utilisation de vecteurs lentiviraux exprimant le facteur viii |
| WO2019195056A1 (fr) | 2018-04-04 | 2019-10-10 | Sigilon Therapeutics, Inc. | Procédés, compositions et éléments implantables comprenant des cellules souches |
| TW202014181A (zh) | 2018-04-04 | 2020-04-16 | 美商希吉隆醫療公司 | 可植入顆粒及相關方法 |
| TW202015723A (zh) | 2018-05-18 | 2020-05-01 | 美商百歐維拉提夫治療公司 | 治療ａ型血友病的方法 |
| MX2021001599A (es) | 2018-08-09 | 2021-07-02 | Bioverativ Therapeutics Inc | Moleculas de acido nucleico y sus usos para la terapia genica no viral. |
| UY38389A (es) | 2018-09-27 | 2020-04-30 | Sigilon Therapeutics Inc | Dispositivos implantables para terapia celular y métodos relacionados |
| TW202115127A (zh) | 2019-06-19 | 2021-04-16 | 美商百歐維拉提夫治療公司 | 治療血友病及低骨質密度之方法及組成物 |
| JP2023537070A (ja) | 2020-08-07 | 2023-08-30 | アミカス セラピューティックス インコーポレイテッド | 小胞を標的とするタンパク質及びその使用 |
| JP2024532262A (ja) | 2021-08-23 | 2024-09-05 | バイオベラティブ セラピューティクス インコーポレイテッド | 最適化第ｖｉｉｉ因子遺伝子 |
| WO2023056331A1 (fr) | 2021-09-30 | 2023-04-06 | Bioverativ Therapeutics Inc. | Acides nucléiques codant pour des polypeptides du facteur viii à immunogénicité réduite |
| WO2024081310A1 (fr) | 2022-10-11 | 2024-04-18 | Sigilon Therapeutics, Inc. | Cellules modifiées et éléments implantables pour le traitement d'une maladie |
| WO2024081309A1 (fr) | 2022-10-11 | 2024-04-18 | Sigilon Therapeutics, Inc. | Cellules modifiées et éléments implantables pour le traitement d'une maladie |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR910006424B1 (ko) * | 1985-08-21 | 1991-08-24 | 인코텍스 비.브이 | 편성브리프(brief) 제조방법 |
| JP2525022B2 (ja) * | 1986-01-03 | 1996-08-14 | ジェネティックス・インスチチュ−ト・インコ−ポレ−テッド | ▲ＶＩＩＩ▼：ｃ因子型タンパク質の改良生産方法 |
| EP0251843A1 (fr) * | 1986-06-06 | 1988-01-07 | Transgene S.A. | Procédé de préparation de facteur VIII à partir de cellules de mammifères |
| JPH0637425B2 (ja) * | 1986-11-11 | 1994-05-18 | 株式会社クラレ | 高沸点（メタ）アクリル酸エステルの製造方法 |
| FR2619314B1 (fr) * | 1987-08-11 | 1990-06-15 | Transgene Sa | Analogue du facteur viii, procede de preparation et composition pharmaceutique le contenant |
| JP2586092B2 (ja) * | 1988-04-06 | 1997-02-26 | 三菱瓦斯化学株式会社 | 多官能（メタ）アクリル酸エステルの製造法 |
| ES2112255T3 (es) * | 1989-11-17 | 1998-04-01 | Novo Nordisk As | Complejos proteicos que tienen actividad del factor viii:c y su produccion. |
-
1989
- 1989-12-15 SE SE8904239A patent/SE465222C5/xx not_active Application Discontinuation
-
1990
- 1990-11-29 NZ NZ236276A patent/NZ236276A/xx unknown
- 1990-12-06 DE DE1999175066 patent/DE19975066I2/de active Active
- 1990-12-06 AT AT97103685T patent/ATE232213T1/de not_active IP Right Cessation
- 1990-12-06 JP JP3501715A patent/JP2644084B2/ja not_active Expired - Lifetime
- 1990-12-06 ES ES97103685T patent/ES2189897T3/es not_active Expired - Lifetime
- 1990-12-06 EP EP02009162A patent/EP1293513A3/fr not_active Withdrawn
- 1990-12-06 WO PCT/SE1990/000809 patent/WO1991009122A1/fr active IP Right Grant
- 1990-12-06 CA CA002071875A patent/CA2071875C/fr not_active Expired - Lifetime
- 1990-12-06 DK DK97103685T patent/DK0786474T3/da active
- 1990-12-06 DE DE69034040T patent/DE69034040T2/de not_active Expired - Lifetime
- 1990-12-06 EP EP91901327A patent/EP0506757B2/fr not_active Expired - Lifetime
- 1990-12-06 AT AT91901327T patent/ATE170219T1/de active
- 1990-12-06 SG SG9603152A patent/SG66753A1/en unknown
- 1990-12-06 AU AU70393/91A patent/AU645539B2/en not_active Expired
- 1990-12-06 ES ES91901327T patent/ES2119769T5/es not_active Expired - Lifetime
- 1990-12-06 DE DE69032600T patent/DE69032600T3/de not_active Expired - Lifetime
- 1990-12-06 DK DK91901327T patent/DK0506757T4/da active
- 1990-12-06 EP EP97103685A patent/EP0786474B1/fr not_active Expired - Lifetime
- 1990-12-06 BR BR909007921A patent/BR9007921A/pt unknown
- 1990-12-14 IE IE451090A patent/IE904510A1/en not_active IP Right Cessation
- 1990-12-14 PT PT96208A patent/PT96208B/pt not_active IP Right Cessation
-
1992
- 1992-06-12 NO NO922325A patent/NO309041B1/no not_active IP Right Cessation
- 1992-06-12 FI FI922745A patent/FI104260B1/fi active
-
1995
- 1995-06-28 HU HU95P/P00489P patent/HU211504A9/hu unknown
-
1998
- 1998-08-14 HK HK98109933A patent/HK1009290A1/xx not_active IP Right Cessation
- 1998-08-14 HK HK99104067A patent/HK1019449A1/xx not_active IP Right Cessation
-
1999
- 1999-10-12 LU LU90457C patent/LU90457I2/fr unknown
- 1999-10-12 NL NL990035C patent/NL990035I2/nl unknown
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| NO309041B1 (no) | DNA-sekvens, rekombinant ekspresjonsvektor og vertscelle omfattende DNA-sekvensen, samt fremgangsmÕte for fremstilling av human faktor VIII-derivat | |
| AU650730B2 (en) | Recombinant human factor VIII derivatives | |
| US5661008A (en) | Recombinant human factor VIII derivatives | |
| US6228620B1 (en) | Protein complexes having factor VIII:C activity and production thereof | |
| JP2513993B2 (ja) | ヒト第ｖｉｉｉ：ｃ因子活性を有する組換えタンパク複合体 | |
| US20060122376A1 (en) | Protein complexes having factor VIII:C activity and production thereof | |
| CA2068728A1 (fr) | Complexes proteiniques presentant une activite du facteur viii:c, et leur production | |
| AU606925B2 (en) | A method for producing factor viii in high yield | |
| ES2319198T3 (es) | Adnc modificado para niveles de expresion altos de factor viii y sus derivados. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MK1K | Patent expired |