KR20070041792A - 신규 시토크롬 p450 모노옥시게나제 및 유기 화합물의산화를 위한 그의 용도 - Google Patents

신규 시토크롬 p450 모노옥시게나제 및 유기 화합물의산화를 위한 그의 용도 Download PDF

Info

Publication number
KR20070041792A
KR20070041792A KR1020077007332A KR20077007332A KR20070041792A KR 20070041792 A KR20070041792 A KR 20070041792A KR 1020077007332 A KR1020077007332 A KR 1020077007332A KR 20077007332 A KR20077007332 A KR 20077007332A KR 20070041792 A KR20070041792 A KR 20070041792A
Authority
KR
South Korea
Prior art keywords
leu
ala
glu
lys
asp
Prior art date
Application number
KR1020077007332A
Other languages
English (en)
Korean (ko)
Inventor
베른하르트 하우어
위르겐 플라이스
울리히 슈바네버크
유타 슈미트
마쿠스 피셔
롤프 슈미트
킹-샨 리
다니엘 아펠
사비네 루츠-발
Original Assignee
바스프 악티엔게젤샤프트
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19935115A external-priority patent/DE19935115A1/de
Priority claimed from DE19955605A external-priority patent/DE19955605A1/de
Priority claimed from DE10014085A external-priority patent/DE10014085A1/de
Application filed by 바스프 악티엔게젤샤프트 filed Critical 바스프 악티엔게젤샤프트
Publication of KR20070041792A publication Critical patent/KR20070041792A/ko

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0071Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/10Nitrogen as only ring hetero atom
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/16Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing two or more hetero rings
    • C12P17/165Heterorings having nitrogen atoms as the only ring heteroatoms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/22Preparation of oxygen-containing organic compounds containing a hydroxy group aromatic
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409Fatty acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/902Oxidoreductases (1.)
    • G01N2333/90245Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Nitrogen And Oxygen As The Only Ring Hetero Atoms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
KR1020077007332A 1999-07-27 2000-07-27 신규 시토크롬 p450 모노옥시게나제 및 유기 화합물의산화를 위한 그의 용도 KR20070041792A (ko)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DE19935115A DE19935115A1 (de) 1999-07-27 1999-07-27 Elektronendonorsystem für Enzyme
DE19935115.5 1999-07-27
DE19955605.9 1999-11-18
DE19955605A DE19955605A1 (de) 1999-11-18 1999-11-18 Neue Cytochrom P450-Monoxygenasen und deren Verwendung zur Oxidation N-heterocyclischer Aromaten
DE10014085.8 2000-03-22
DE10014085A DE10014085A1 (de) 2000-03-22 2000-03-22 Neue Cytochrom P450-Monooxygenasen und deren Verwendung zur Oxidation von organischen Verbindungen

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
KR1020027001132A Division KR100740368B1 (ko) 1999-07-27 2000-07-27 신규 시토크롬 p450 모노옥시게나제 및 유기 화합물의산화를 위한 그의 용도

Publications (1)

Publication Number Publication Date
KR20070041792A true KR20070041792A (ko) 2007-04-19

Family

ID=27213745

Family Applications (2)

Application Number Title Priority Date Filing Date
KR1020027001132A KR100740368B1 (ko) 1999-07-27 2000-07-27 신규 시토크롬 p450 모노옥시게나제 및 유기 화합물의산화를 위한 그의 용도
KR1020077007332A KR20070041792A (ko) 1999-07-27 2000-07-27 신규 시토크롬 p450 모노옥시게나제 및 유기 화합물의산화를 위한 그의 용도

Family Applications Before (1)

Application Number Title Priority Date Filing Date
KR1020027001132A KR100740368B1 (ko) 1999-07-27 2000-07-27 신규 시토크롬 p450 모노옥시게나제 및 유기 화합물의산화를 위한 그의 용도

Country Status (18)

Country Link
US (1) US7960155B1 (de)
EP (1) EP1196605B1 (de)
JP (1) JP4791664B2 (de)
KR (2) KR100740368B1 (de)
CN (1) CN1183252C (de)
AT (1) ATE409232T1 (de)
AU (1) AU780694B2 (de)
BR (1) BR0012772B1 (de)
CA (1) CA2380186C (de)
DE (1) DE50015373D1 (de)
EE (1) EE200200048A (de)
ES (1) ES2311470T3 (de)
HU (1) HU229450B1 (de)
IL (2) IL147580A0 (de)
MY (1) MY126592A (de)
NO (1) NO20020380L (de)
UA (1) UA76701C2 (de)
WO (1) WO2001007630A1 (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE371028T1 (de) * 1999-07-27 2007-09-15 Basf Ag Modifizierte cytochrom p450-monooxygenasen
EP1303614A2 (de) 2000-07-18 2003-04-23 National Research Council Of Canada Klonierung, sequenzierung und expression eines comamonas-spezifischen, cyclopentanon 1,2-monooxygenase-kodierenden gens in escherichia coli
DE10051175A1 (de) 2000-10-16 2002-05-02 Basf Ag Cytochrom P450 Monoxygenasen aus thermophilen Bakterien
DE10321082A1 (de) * 2003-05-09 2004-11-25 Basf Ag Verfahren zur Herstellung eines Hydroxylierungskatalysators und seine Verwendung
KR100564158B1 (ko) * 2004-07-23 2006-03-24 주식회사 진켐 싸이토크롬 p450 효소 및 이를 코딩하는 유전자
DE102004042102A1 (de) * 2004-08-30 2006-03-09 GESELLSCHAFT FüR BIOTECHNOLOGISCHE FORSCHUNG MBH (GBF) Verfahren zur regio-selektiven Oxidation
US8715988B2 (en) * 2005-03-28 2014-05-06 California Institute Of Technology Alkane oxidation by modified hydroxylases
CN100400652C (zh) * 2005-07-21 2008-07-09 南开大学 高效降解芘基因工程菌及其构建
CN100429314C (zh) * 2006-04-18 2008-10-29 浙江大学 能催化吲哚生成靛蓝的质粒pET28a(+)-P450 BM3-gdh0310、制备方法及其用途
EP2047029A1 (de) * 2006-07-27 2009-04-15 Ciba Holding Inc. Biokatalytische hydrophilierung von polyolefinen
GB0719620D0 (en) 2007-10-08 2007-11-14 Isis Innovation Mutant Enzymes
KR100975404B1 (ko) * 2008-02-28 2010-08-11 주식회사 종합건축사사무소근정 블록담장용 지주 및 이의 시공방법
CN101333521B (zh) * 2008-04-25 2010-12-15 浙江大学 细胞色素p450bm-3d168w变体酶以及应用其制备靛玉红的方法
DE102008054918A1 (de) * 2008-12-18 2010-07-01 Evonik Degussa Gmbh Verfahren zur enzymatischen Umsetzung von Alkanen
CN101580821B (zh) * 2009-01-19 2011-04-27 广东省微生物研究所 用于制备抗癌药物靛玉红的2-萘酸单加氧酶
EP2426198A1 (de) 2010-09-03 2012-03-07 B.R.A.I.N. Biotechnology Research And Information Network AG Cytochrome P450 Monooxygenase Varianten
CN102154234A (zh) * 2011-01-18 2011-08-17 浙江大学 具有多环芳烃羟化酶活性的细胞色素p450单加氧酶
CN103146596A (zh) * 2012-12-14 2013-06-12 徐州工程学院 一种生产靛蓝色素的专用菌株
WO2014100251A1 (en) * 2012-12-18 2014-06-26 California Institute Of Technology A cytochrome p450-based biodesulfurization pathway
US10975243B2 (en) 2018-12-28 2021-04-13 Industrial Technology Research Institute Genetically modified microorganism and method for producing indigo dye
EP3858986A1 (de) 2020-02-03 2021-08-04 Bayer Aktiengesellschaft P450-bm3-monooxygenase-varianten zur c19-hydroxylierung von steroiden
US11345818B1 (en) 2020-12-29 2022-05-31 Industrial Technology Research Institute Dye for fiber and dyeing method
WO2024004921A1 (ja) * 2022-06-27 2024-01-04 天野エンザイム株式会社 ポリペプチド、オキシゲナーゼ及びこれらの応用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9422205D0 (en) * 1994-11-03 1994-12-21 British Gas Plc Enzyme mutant and method
DE19507546C2 (de) 1995-03-03 2001-05-03 Max Delbrueck Centrum Verfahren zur regioselektiven Hydroxylierung von langkettigen Alkanen, Fettsäuren und anderen Alkylverbindungen
US5691171A (en) 1995-10-23 1997-11-25 Board Of Trustees Operating Michigan State University Method for production of indigo and indirubin dyes
CN1212015A (zh) * 1995-11-01 1999-03-24 Bg公开有限公司 突变单加氧酶细胞色素P450cam
US6605430B1 (en) * 1998-08-12 2003-08-12 Maxygen, Inc. DNA shuffling of monooxygenase genes for production of industrial chemicals
GB9825421D0 (en) 1998-11-19 1999-01-13 Isis Innovation Process for oxidising terpenes
ATE371028T1 (de) * 1999-07-27 2007-09-15 Basf Ag Modifizierte cytochrom p450-monooxygenasen

Also Published As

Publication number Publication date
HU229450B1 (hu) 2013-12-30
EE200200048A (et) 2003-04-15
ATE409232T1 (de) 2008-10-15
MY126592A (en) 2006-10-31
BR0012772B1 (pt) 2013-05-28
JP2003521889A (ja) 2003-07-22
HUP0202074A2 (en) 2002-09-28
NO20020380D0 (no) 2002-01-24
CA2380186A1 (en) 2001-02-01
UA76701C2 (uk) 2006-09-15
CN1183252C (zh) 2005-01-05
DE50015373D1 (de) 2008-11-06
JP4791664B2 (ja) 2011-10-12
NO20020380L (no) 2002-03-22
ES2311470T3 (es) 2009-02-16
AU780694B2 (en) 2005-04-14
KR20020016924A (ko) 2002-03-06
CA2380186C (en) 2012-03-13
BR0012772A (pt) 2002-04-02
EP1196605B1 (de) 2008-09-24
US7960155B1 (en) 2011-06-14
KR100740368B1 (ko) 2007-07-16
CN1365393A (zh) 2002-08-21
EP1196605A1 (de) 2002-04-17
WO2001007630A1 (de) 2001-02-01
IL147580A (en) 2013-12-31
AU6830700A (en) 2001-02-13
IL147580A0 (en) 2002-08-14
HUP0202074A3 (en) 2010-01-28

Similar Documents

Publication Publication Date Title
KR100740368B1 (ko) 신규 시토크롬 p450 모노옥시게나제 및 유기 화합물의산화를 위한 그의 용도
Meyer et al. Changing the substrate reactivity of 2-hydroxybiphenyl 3-monooxygenase from Pseudomonas azelaica HBP1 by directed evolution
JP3045540B2 (ja) 酵素類および薬物検出におけるそれらの使用
JP4251554B2 (ja) 大腸菌における放線菌由来チトクロームp−450遺伝子の発現系
US7553648B2 (en) Cytochrome P450 monooxygenases consisting of thermophilic bacteria
JP2003512078A (ja) 芳香族アルデヒド及び/又はカルボン酸の微生物学的製造方法
ITo et al. An organic solvent resistant tyrosinase from Streptomyces sp. REN-21: purification and characterization
US5691171A (en) Method for production of indigo and indirubin dyes
KR101783243B1 (ko) 스트렙토마이세스 카틀레야 유래 CYP102_scat를 이용하는 바이오 인디고 생산 방법
KR101780510B1 (ko) 케토 화합물의 입체선택적 효소적 환원 방법
RU2285044C2 (ru) Новые цитохром р450-монооксигеназы и их применение для окисления органических соединений
WO2013076258A2 (en) P450 bm3 mutants and their use for regio- and stereoselective hydroxylation of alpha-and beta-ionone
Kagami et al. Protein engineering on biphenyl dioxygenase for conferring activity to convert 7-hydroxyflavone and 5, 7-dihydroxyflavone (chrysin)
KR100769932B1 (ko) 시토크롬 씨 옥시다아제 효소 복합체
JP4800000B2 (ja) 改変型芳香環ジオキシゲナーゼ及び水酸化フラボン類化合物の製造法
JP2006500922A (ja) カロチノイドの生物変換方法
KR20230036347A (ko) CYP154C3s 효소를 이용한 스테로이드의 하이드록시화 생물전환 방법
WO2023043946A2 (en) Cannabinoid production in bacteria
JP2003061682A (ja) 還元酵素遺伝子及びその利用
DE10014085A1 (de) Neue Cytochrom P450-Monooxygenasen und deren Verwendung zur Oxidation von organischen Verbindungen
JP2003070493A (ja) 酢酸菌を用いるシキミ酸の製造法およびキナ酸脱水素酵素
JPWO2004078988A1 (ja) 芳香族ジオールの製造方法

Legal Events

Date Code Title Description
A107 Divisional application of patent
A201 Request for examination
E902 Notification of reason for refusal
E601 Decision to refuse application