BR0012772A - Citocromo p450 monooxigenase, sequência de ácido nucleico, constructo de expressão, vetor, microorganismo recombinante, processo para a oxidação microbiológica de um composto aromático mono ou polinuclear n- ou s-heterocìclico, processo para a produção microbiológica de indigo e/ou indirubin, biorreator, uso de uma citocromo p450 monooxigenase, de um vetor, ou de um microorganismo, e, uso de um microorganismo que produz citocromo p450 oxidante de indol - Google Patents

Citocromo p450 monooxigenase, sequência de ácido nucleico, constructo de expressão, vetor, microorganismo recombinante, processo para a oxidação microbiológica de um composto aromático mono ou polinuclear n- ou s-heterocìclico, processo para a produção microbiológica de indigo e/ou indirubin, biorreator, uso de uma citocromo p450 monooxigenase, de um vetor, ou de um microorganismo, e, uso de um microorganismo que produz citocromo p450 oxidante de indol

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Publication number
BR0012772A
BR0012772A BR0012772-8A BR0012772A BR0012772A BR 0012772 A BR0012772 A BR 0012772A BR 0012772 A BR0012772 A BR 0012772A BR 0012772 A BR0012772 A BR 0012772A
Authority
BR
Brazil
Prior art keywords
cytochrome
microorganism
monooxygenase
vector
microbiological
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
BR0012772-8A
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English (en)
Other versions
BR0012772B1 (pt
Inventor
Bernhard Hauer
Juergen Pleiss
Ulrich Schwaneberg
Jutta Schmitt
Markus Fischer
Rolf Schmid
Qing-Shan Li
Sabine Lutz-Wahl
Daniel Appel
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BASF SE
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BASF SE
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Filing date
Publication date
Priority claimed from DE19935115A external-priority patent/DE19935115A1/de
Priority claimed from DE19955605A external-priority patent/DE19955605A1/de
Priority claimed from DE10014085A external-priority patent/DE10014085A1/de
Application filed by BASF SE filed Critical BASF SE
Publication of BR0012772A publication Critical patent/BR0012772A/pt
Publication of BR0012772B1 publication Critical patent/BR0012772B1/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/10—Nitrogen as only ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/16—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing two or more hetero rings
    • C12P17/165—Heterorings having nitrogen atoms as the only ring heteroatoms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/22—Preparation of oxygen-containing organic compounds containing a hydroxy group aromatic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/90245—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Nitrogen And Oxygen As The Only Ring Hetero Atoms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

"CITOCROMO P450 MONOOXIGENASE, SEQuêNCIA DE áCIDO NUCLEICO, CONSTRUCTO DE EXPRESSãO, VETOR, MICROORGANISMO RECOMBINANTE, PROCESSO PARA A OXIDAçãO MICROBIOLóGICA DE UM COMPOSTO AROMáTICO MONO OU POLINUCLEAR N- OU S-HETEROCìCLICO, PROCESSO PARA A PRODUçãO MICROBIOLóGICA DE INDIGO E/OU INDIRUBIN, BIORREATOR, USO DE UMA CITOCROMO P450 MONOOXIGENASE, DE UM VETOR, OU DE UM MICROORGANISMO, E, USO DE UM MICROORGANISMO QUE PRODUZ CITOCROMO P450 OXIDANTE DE INDOL". A invenção refere-se às novas citocromo P450 monooxigenases compreendendo uma especificidade de substrato modificada, às seq³ências de nucleotídeos que codificam as mesmas, aos vetores e constructos de expressão contendo estas seq³ências, e aos microorganismos transformados com as mesmas. A invenção também se refere aos métodos para oxidar microbiologicamente diferentes substratos orgânicos, tais como métodos para produzir indigo e indirubina.
BRPI0012772-8A 1999-07-27 2000-07-27 citocromo p450 monooxigenase, sequÊncia de Ácido nucleico, constructo de expressço, vetor, microorganismo recombinante, processos para a oxidaÇço microbiolàgica de um composto aromÁtico mono ou polinuclear n-, o- ou s-heterocÍclico e para a produÇço microbiolàgica de indigo e/ou indirubin, usos de uma citocromo p450 monooxigenase, de um vetor ou de um microorganismo recombinante e de um microorganismo que produz uma citocromo p450 oxidante de indol, e, biorreator. BR0012772B1 (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE19935115A DE19935115A1 (de) 1999-07-27 1999-07-27 Elektronendonorsystem für Enzyme
DE19955605A DE19955605A1 (de) 1999-11-18 1999-11-18 Neue Cytochrom P450-Monoxygenasen und deren Verwendung zur Oxidation N-heterocyclischer Aromaten
DE10014085A DE10014085A1 (de) 2000-03-22 2000-03-22 Neue Cytochrom P450-Monooxygenasen und deren Verwendung zur Oxidation von organischen Verbindungen
PCT/EP2000/007253 WO2001007630A1 (de) 1999-07-27 2000-07-27 Neue cytochrom p450-monooxygenasen und deren verwendung zur oxidation von organischen verbindungen

Publications (2)

Publication Number Publication Date
BR0012772A true BR0012772A (pt) 2002-04-02
BR0012772B1 BR0012772B1 (pt) 2013-05-28

Family

ID=27213745

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0012772-8A BR0012772B1 (pt) 1999-07-27 2000-07-27 citocromo p450 monooxigenase, sequÊncia de Ácido nucleico, constructo de expressço, vetor, microorganismo recombinante, processos para a oxidaÇço microbiolàgica de um composto aromÁtico mono ou polinuclear n-, o- ou s-heterocÍclico e para a produÇço microbiolàgica de indigo e/ou indirubin, usos de uma citocromo p450 monooxigenase, de um vetor ou de um microorganismo recombinante e de um microorganismo que produz uma citocromo p450 oxidante de indol, e, biorreator.

Country Status (18)

Country Link
US (1) US7960155B1 (pt)
EP (1) EP1196605B1 (pt)
JP (1) JP4791664B2 (pt)
KR (2) KR20070041792A (pt)
CN (1) CN1183252C (pt)
AT (1) ATE409232T1 (pt)
AU (1) AU780694B2 (pt)
BR (1) BR0012772B1 (pt)
CA (1) CA2380186C (pt)
DE (1) DE50015373D1 (pt)
EE (1) EE200200048A (pt)
ES (1) ES2311470T3 (pt)
HU (1) HU229450B1 (pt)
IL (2) IL147580A0 (pt)
MY (1) MY126592A (pt)
NO (1) NO20020380L (pt)
UA (1) UA76701C2 (pt)
WO (1) WO2001007630A1 (pt)

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AU784049B2 (en) * 1999-07-27 2006-01-19 Basf Aktiengesellschaft Modified cytochrome P450 monooxygenases
WO2002006452A2 (en) 2000-07-18 2002-01-24 National Research Council Of Canada Cloning, sequencing and expression of a comamonas cyclopentanone 1,2-monooxygenase-encoding gene in escherichia coli
DE10051175A1 (de) 2000-10-16 2002-05-02 Basf Ag Cytochrom P450 Monoxygenasen aus thermophilen Bakterien
DE10321082A1 (de) * 2003-05-09 2004-11-25 Basf Ag Verfahren zur Herstellung eines Hydroxylierungskatalysators und seine Verwendung
WO2006009334A1 (en) * 2004-07-23 2006-01-26 Genechem Inc. Cytochrome p450 enzyme and the gene encoding the same
DE102004042102A1 (de) * 2004-08-30 2006-03-09 GESELLSCHAFT FüR BIOTECHNOLOGISCHE FORSCHUNG MBH (GBF) Verfahren zur regio-selektiven Oxidation
US8715988B2 (en) * 2005-03-28 2014-05-06 California Institute Of Technology Alkane oxidation by modified hydroxylases
CN100400652C (zh) * 2005-07-21 2008-07-09 南开大学 高效降解芘基因工程菌及其构建
CN100429314C (zh) * 2006-04-18 2008-10-29 浙江大学 能催化吲哚生成靛蓝的质粒pET28a(+)－P450 BM3－gdh0310、制备方法及其用途
US20100047533A1 (en) * 2006-07-27 2010-02-25 Eva Almansa Biocatalytic Hydrophilization of Polyolefines
GB0719620D0 (en) * 2007-10-08 2007-11-14 Isis Innovation Mutant Enzymes
KR100975404B1 (ko) * 2008-02-28 2010-08-11 주식회사 종합건축사사무소근정 블록담장용 지주 및 이의 시공방법
CN101333521B (zh) * 2008-04-25 2010-12-15 浙江大学 细胞色素p450bm-3d168w变体酶以及应用其制备靛玉红的方法
DE102008054918A1 (de) * 2008-12-18 2010-07-01 Evonik Degussa Gmbh Verfahren zur enzymatischen Umsetzung von Alkanen
CN101580821B (zh) * 2009-01-19 2011-04-27 广东省微生物研究所 用于制备抗癌药物靛玉红的2-萘酸单加氧酶
EP2426198A1 (en) 2010-09-03 2012-03-07 B.R.A.I.N. Biotechnology Research And Information Network AG Cytochrome P450 monooxygenase variants
CN102154234A (zh) * 2011-01-18 2011-08-17 浙江大学 具有多环芳烃羟化酶活性的细胞色素p450单加氧酶
CN103146596A (zh) * 2012-12-14 2013-06-12 徐州工程学院 一种生产靛蓝色素的专用菌株
WO2014100251A1 (en) * 2012-12-18 2014-06-26 California Institute Of Technology A cytochrome p450-based biodesulfurization pathway
EP3607076A1 (en) 2017-04-07 2020-02-12 DSM IP Assets B.V. Regioselective hydroxylation of isophorone and further conversion to ketoisophorone
US10975243B2 (en) 2018-12-28 2021-04-13 Industrial Technology Research Institute Genetically modified microorganism and method for producing indigo dye
EP3858986A1 (en) 2020-02-03 2021-08-04 Bayer Aktiengesellschaft P450 bm3 monooxygenase variants for c19-hydroxylation of steroids
US11345818B1 (en) 2020-12-29 2022-05-31 Industrial Technology Research Institute Dye for fiber and dyeing method
WO2024004921A1 (ja) * 2022-06-27 2024-01-04 天野エンザイム株式会社 ポリペプチド、オキシゲナーゼ及びこれらの応用

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DE19507546C2 (de) 1995-03-03 2001-05-03 Max Delbrueck Centrum Verfahren zur regioselektiven Hydroxylierung von langkettigen Alkanen, Fettsäuren und anderen Alkylverbindungen
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Also Published As

Publication number Publication date
NO20020380L (no) 2002-03-22
US7960155B1 (en) 2011-06-14
NO20020380D0 (no) 2002-01-24
UA76701C2 (uk) 2006-09-15
ATE409232T1 (de) 2008-10-15
CA2380186A1 (en) 2001-02-01
ES2311470T3 (es) 2009-02-16
MY126592A (en) 2006-10-31
WO2001007630A1 (de) 2001-02-01
EP1196605A1 (de) 2002-04-17
IL147580A0 (en) 2002-08-14
JP4791664B2 (ja) 2011-10-12
EE200200048A (et) 2003-04-15
JP2003521889A (ja) 2003-07-22
KR100740368B1 (ko) 2007-07-16
HUP0202074A3 (en) 2010-01-28
AU780694B2 (en) 2005-04-14
BR0012772B1 (pt) 2013-05-28
CN1183252C (zh) 2005-01-05
CN1365393A (zh) 2002-08-21
IL147580A (en) 2013-12-31
KR20070041792A (ko) 2007-04-19
DE50015373D1 (de) 2008-11-06
EP1196605B1 (de) 2008-09-24
CA2380186C (en) 2012-03-13
HU229450B1 (hu) 2013-12-30
HUP0202074A2 (en) 2002-09-28
KR20020016924A (ko) 2002-03-06
AU6830700A (en) 2001-02-13

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B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time

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B24J Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12)

Free format text: EM VIRTUDE DA EXTINCAO PUBLICADA NA RPI 2594 DE 24-09-2020 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDA A EXTINCAO DA PATENTE E SEUS CERTIFICADOS, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.