KR102110876B1 - 바이러스 유사 입자의 정제 - Google Patents

바이러스 유사 입자의 정제 Download PDF

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Publication number
KR102110876B1
KR102110876B1 KR1020157001131A KR20157001131A KR102110876B1 KR 102110876 B1 KR102110876 B1 KR 102110876B1 KR 1020157001131 A KR1020157001131 A KR 1020157001131A KR 20157001131 A KR20157001131 A KR 20157001131A KR 102110876 B1 KR102110876 B1 KR 102110876B1
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vlp
solution
vlps
filtrate
chromatography
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KR20150051988A (ko
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로스 테일러
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다케다 백신즈 인코포레이티드
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/20—Antivirals for DNA viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/16011—Caliciviridae
    • C12N2770/16022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/16011—Caliciviridae
    • C12N2770/16023—Virus like particles [VLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/16011—Caliciviridae
    • C12N2770/16051—Methods of production or purification of viral material

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Virology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Communicable Diseases (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Oncology (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Analytical Chemistry (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
KR1020157001131A 2012-06-22 2013-06-24 바이러스 유사 입자의 정제 Active KR102110876B1 (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261663218P 2012-06-22 2012-06-22
US61/663,218 2012-06-22
US201361794086P 2013-03-15 2013-03-15
US61/794,086 2013-03-15
PCT/US2013/047249 WO2013192604A1 (en) 2012-06-22 2013-06-24 Purification of virus like particles

Publications (2)

Publication Number Publication Date
KR20150051988A KR20150051988A (ko) 2015-05-13
KR102110876B1 true KR102110876B1 (ko) 2020-05-14

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US (3) US10167320B2 (enExample)
EP (1) EP2864478B1 (enExample)
JP (1) JP6553509B2 (enExample)
KR (1) KR102110876B1 (enExample)
CN (2) CN104583393A (enExample)
AR (1) AR091538A1 (enExample)
AU (1) AU2013277959B2 (enExample)
CA (1) CA2879815A1 (enExample)
IN (1) IN2015DN00089A (enExample)
JO (1) JOP20130186B1 (enExample)
MX (1) MX366234B (enExample)
SG (2) SG11201408459VA (enExample)
TW (1) TWI647310B (enExample)
UY (1) UY34875A (enExample)
WO (1) WO2013192604A1 (enExample)

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SG184733A1 (en) 2007-09-18 2012-10-30 Ligocyte Pharmaceuticals Inc Method of conferring a protective immune response to norovirus
DK3299030T3 (da) 2011-07-11 2022-09-05 Takeda Vaccines Inc Parenterale norovirus-vaccineformuleringer
JOP20130186B1 (ar) 2012-06-22 2021-08-17 Takeda Vaccines Montana Inc تنقية الجزيئات الشبيهة بالفيروسات
US10072250B2 (en) * 2013-07-11 2018-09-11 Takara Bio Inc. Method for manufacturing non-enveloped virus
CN106103720A (zh) 2013-10-03 2016-11-09 武田疫苗股份有限公司 自细胞系检测和除去弹状病毒的方法
CN103981084B (zh) * 2014-05-29 2015-11-18 黑龙江省科学院微生物研究所 水中病毒分离提取装置及水中札幌病毒的提取方法
EP3243904A4 (en) * 2015-01-09 2018-06-27 Takara Bio Inc. Method for producing non-enveloped viral particles
JP6893174B2 (ja) * 2015-01-16 2021-06-23 タケダ ワクチン，インコーポレイテッド 粒子に含まれる逆転写酵素活性の検出
CN105907729B (zh) * 2015-05-05 2019-08-16 广东东阳光药业有限公司 一种制备人乳头瘤病毒l1蛋白类病毒样颗粒的方法
CN107034198B (zh) * 2015-07-15 2021-03-09 长春百克生物科技股份公司 一种嵌合诺如病毒p颗粒及其制备和应用
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EP4382183A3 (en) 2017-03-06 2024-08-21 Guangzhou Realbenefitspot Pharmaceutical Co., Ltd. Methods of producing and characterizing virus vaccine and virus vaccine composition
EP3420076B1 (en) * 2017-03-06 2024-02-21 Guangzhou Realbenefitspot Pharmaceutical Co., Ltd. Methods of producing and characterizing virus vaccine and virus vaccine composition
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CN108676781A (zh) * 2018-05-18 2018-10-19 山东信得动物疫苗有限公司 一种重组禽流感病毒的工业化纯化方法
CN111848749A (zh) * 2018-05-23 2020-10-30 中国农业科学院兰州兽医研究所 猪细小病毒VLPs抗体检测试剂盒及其制备方法、应用
EP3938378B1 (en) 2019-03-12 2023-08-02 Icon Genetics GmbH Norovirus-like particles with improved stability
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CN116789773B (zh) * 2022-11-30 2024-02-20 华北制药金坦生物技术股份有限公司 一种胞内表达病毒样颗粒的粗纯方法
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Also Published As

Publication number Publication date
SG11201408459VA (en) 2015-01-29
CN114085273A (zh) 2022-02-25
MX366234B (es) 2019-07-03
JP6553509B2 (ja) 2019-07-31
IN2015DN00089A (enExample) 2015-05-29
US20220213147A1 (en) 2022-07-07
TW201406956A (zh) 2014-02-16
EP2864478B1 (en) 2020-12-09
CA2879815A1 (en) 2013-12-27
UY34875A (es) 2013-12-31
US10167320B2 (en) 2019-01-01
AU2013277959A1 (en) 2015-01-22
KR20150051988A (ko) 2015-05-13
MX2014015842A (es) 2015-09-07
EP2864478A4 (en) 2015-12-02
EP2864478A1 (en) 2015-04-29
SG10201610753YA (en) 2017-02-27
CN114085273B (zh) 2024-07-12
JP2015521628A (ja) 2015-07-30
US11091519B2 (en) 2021-08-17
AR091538A1 (es) 2015-02-11
AU2013277959B2 (en) 2019-05-16
US20140004145A1 (en) 2014-01-02
JOP20130186B1 (ar) 2021-08-17
US12286456B2 (en) 2025-04-29
HK1210210A1 (en) 2016-04-15
US20190169236A1 (en) 2019-06-06
TWI647310B (zh) 2019-01-11
WO2013192604A1 (en) 2013-12-27
CN104583393A (zh) 2015-04-29

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