KR101424812B1 - 핵산 증폭을 위한 프라이머, 프로브 및 방법 - Google Patents

핵산 증폭을 위한 프라이머, 프로브 및 방법 Download PDF

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KR101424812B1
KR101424812B1 KR1020077011244A KR20077011244A KR101424812B1 KR 101424812 B1 KR101424812 B1 KR 101424812B1 KR 1020077011244 A KR1020077011244 A KR 1020077011244A KR 20077011244 A KR20077011244 A KR 20077011244A KR 101424812 B1 KR101424812 B1 KR 101424812B1
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probe
primer
pcr
fluorescence
late
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KR20070090159A (ko
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로렌스 제이. 왕
존 라이스
아킬레스 제이. 산체스
케네쓰 피어스
제시 솔크
아더 레이스
크리스티나 하츠혼
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브랜데이스 유니버시티
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
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    • C12N15/09—Recombinant DNA-technology
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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KR1020077011244A 2004-10-18 2005-10-17 핵산 증폭을 위한 프라이머, 프로브 및 방법 Expired - Fee Related KR101424812B1 (ko)

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US61965404P 2004-10-18 2004-10-18
US60/619,654 2004-10-18
PCT/US2005/037667 WO2006044994A2 (en) 2004-10-18 2005-10-17 Primers, probes and methods for nucleic acid amplification

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KR1020137025318A Division KR101447405B1 (ko) 2004-10-18 2005-10-17 핵산 증폭을 위한 프라이머, 프로브 및 방법
KR1020137002325A Division KR101347951B1 (ko) 2004-10-18 2005-10-17 핵산 증폭을 위한 프라이머, 프로브 및 방법

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KR20070090159A KR20070090159A (ko) 2007-09-05
KR101424812B1 true KR101424812B1 (ko) 2014-08-07

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KR1020077011244A Expired - Fee Related KR101424812B1 (ko) 2004-10-18 2005-10-17 핵산 증폭을 위한 프라이머, 프로브 및 방법
KR1020137002325A Expired - Fee Related KR101347951B1 (ko) 2004-10-18 2005-10-17 핵산 증폭을 위한 프라이머, 프로브 및 방법

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US (3) US7632642B2 (enExample)
EP (3) EP2351766B1 (enExample)
JP (2) JP5543065B2 (enExample)
KR (3) KR101447405B1 (enExample)
CN (1) CN101076607B (enExample)
AT (1) ATE494295T1 (enExample)
AU (1) AU2005295298B2 (enExample)
BR (1) BRPI0517105B1 (enExample)
CA (3) CA2855748C (enExample)
DE (1) DE602005025791D1 (enExample)
DK (1) DK1805199T3 (enExample)
ES (3) ES2668467T3 (enExample)
NO (1) NO20072506L (enExample)
PL (1) PL1805199T3 (enExample)
PT (1) PT1805199E (enExample)
RU (1) RU2460804C2 (enExample)
WO (1) WO2006044994A2 (enExample)

Families Citing this family (84)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2460804C2 (ru) * 2004-10-18 2012-09-10 Брандейс Юнивесити Способ гомогенной детекции по меньшей мере одного продукта одноцепочечной амплификации
CN100487432C (zh) * 2006-06-22 2009-05-13 上海交通大学 采用分子信标对核酸信号进行恒温放大与检测的方法
AU2008275597A1 (en) 2007-07-12 2009-01-15 Smiths Detection Inc. Fiber optic detection system
CA2740973A1 (en) 2007-10-23 2009-04-30 Stratos Genomics Inc. High throughput nucleic acid sequencing by spacing
US8538733B2 (en) * 2008-01-25 2013-09-17 Life Technologies Corporation Methods for the analysis of dissociation melt curve data
US20100129796A1 (en) * 2008-11-24 2010-05-27 Micah Halpern Dye probe fluorescence resonance energy transfer genotyping
US9856522B2 (en) 2008-12-30 2018-01-02 Stmicroelectronics S.R.L. Method, microreactor and apparatus for carrying out real-time nucleic acid amplification
FR2940805B1 (fr) * 2009-01-05 2015-10-16 Biomerieux Sa Procede d'amplification et/ou de detection d'acides nucleiques, kits et utilisations de ce procede
JP5423006B2 (ja) * 2009-01-20 2014-02-19 ソニー株式会社 プライマー評価方法、プライマー評価プログラム及びリアルタイムｐｃｒ装置
US8039215B2 (en) * 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
EP2406394B1 (en) * 2009-03-12 2014-01-08 Brandeis University Reagents and methods for pcr
US8674080B2 (en) 2009-04-09 2014-03-18 Roche Molecular Systems, Inc. Dye composition for liquid transfer control
FI20095514A0 (fi) * 2009-05-07 2009-05-07 Expression Analytics Oy Menetelmä, laitteisto ja tietokoneohjelmatuote PCR-tuotteiden kvantifioimiseksi
DE102009026651A1 (de) * 2009-06-02 2011-02-17 Großmann, Kay, Dr. rer. nat. (Chemie) Verfahren und Messvorrichtung zur qualitativen und quantitativen Analyse von Körperflüssigkeiten
AR077840A1 (es) * 2009-08-11 2011-09-28 Univ Brandeis Ensayos de deteccion e identificacion de especies y tipos de staphylococcus
AR077841A1 (es) * 2009-08-11 2011-09-28 Univ Brandeis Metodos kits y composiciones de deteccion de acido nucleico a multiples temperaturas, con sonda unica
CN102471364B (zh) * 2009-08-14 2015-01-14 株式会社日立制作所 基因序列分析的方法和试剂
EP2491144B1 (en) 2009-10-21 2015-09-30 Brandeis University Methods for analyzing single-stranded nucleic acid sequences
AU2010315303B2 (en) * 2009-11-03 2015-08-06 Htg Molecular Diagnostics, Inc. Quantitative Nuclease Protection Sequencing (qNPS)
EP2569447A4 (en) * 2010-05-14 2013-11-27 Fluidigm Corp ASSAYS FOR THE DETECTION OF GENOTYPES, MUTATIONS OR ANEUPLOIDY
IT1405994B1 (it) * 2010-06-22 2014-02-06 Euroclone Spa Metodo ad elevata sensibilita' per rilevare un acido nucleico target in un campione
US20120058481A1 (en) * 2010-08-20 2012-03-08 Life Technologies Corporation Quantitative Real Time PCR Assay Using FRET Dual-Labeled Primers
EP2614157A4 (en) * 2010-09-08 2014-03-26 Univ Brandeis Compositions and methods for nucleic acid based diagnostic assays for variable sequence targets
WO2012040387A1 (en) 2010-09-24 2012-03-29 The Board Of Trustees Of The Leland Stanford Junior University Direct capture, amplification and sequencing of target dna using immobilized primers
US20130295570A1 (en) * 2010-10-07 2013-11-07 Brandeis University Compositions and methods for nucleic acid based diagnostic assays
GB201017978D0 (en) 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
ES2606145T3 (es) 2010-11-10 2017-03-22 Brandeis University Procedimientos y kits para la detección e identificación de micobacterias
US9637790B2 (en) 2010-12-03 2017-05-02 Brandeis University Detecting mutations in DNA
US20140004504A1 (en) * 2010-12-10 2014-01-02 Brandeis University Compositions and methods for the detection and analysis of african swine fever virus
CA2965074A1 (en) 2010-12-27 2012-07-05 Abbott Molecular Inc. Quantitating high titer samples by digital pcr
WO2012095748A2 (en) * 2011-01-14 2012-07-19 Smiths Detection - Watford Limited Single tube quantitative polymerase chain reaction (pcr)
WO2012151111A1 (en) 2011-05-04 2012-11-08 Htg Molecular Diagnostics, Inc. Quantitative nuclease protection assay (qnpa) and sequencing (qnps) improvements
WO2013019759A1 (en) * 2011-07-30 2013-02-07 The Regents Of The University Of California Enzymatic preparation of 10 base to 50 kb double-strand dna reagent for sequencing with a nanopore-polymerase sequencing device
US20130052650A1 (en) 2011-08-29 2013-02-28 Thermo Fisher Scientific Inc. Dye blends
CN102321765B (zh) * 2011-09-08 2014-04-02 厦门基科生物科技有限公司 一种实时荧光pcr方法及用途
CA2849023C (en) 2011-09-15 2022-07-19 David A. Shafer Probe:antiprobe compositions for high specificity dna or rna detection
JP5570657B2 (ja) * 2011-10-31 2014-08-13 アークレイ株式会社 遺伝子存在量の測定方法
EP4361608A3 (en) 2012-02-03 2024-07-24 California Institute of Technology Signal encoding and decoding in multiplexed biochemical assays
WO2013131083A1 (en) 2012-03-02 2013-09-06 Winthrop-University Hospital METHOD FOR USING PROBE BASED PCR DETECTION TO MEASURE THE LEVELS OF CIRCULATING DEMETHYLATED β CELL DERIVED DNA AS A MEASURE OF β CELL LOSS IN DIABETES
CN102643910B (zh) * 2012-04-10 2014-10-15 中国人民解放军第三军医大学第一附属医院 非对称多色荧光发夹探针链反应在病原菌检测中的应用
EP3901243A1 (en) * 2012-08-03 2021-10-27 California Institute of Technology Multiplexing and quantification in pcr with reduced hardware and requirements
CA2885195A1 (en) * 2012-09-17 2014-03-20 Brandeis University Combination of dsdna binding dye and probes for characterization of ssdna sequences
EP2959016A4 (en) * 2013-02-21 2016-10-12 Toma Biosciences Inc METHOD, COMPOSITIONS AND NUCLEIC ACID ANALYSIS KITS
EP2971095B1 (en) * 2013-03-12 2019-11-20 Life Technologies Corporation Universal reporter-based genotyping methods, reaction mixture and kit
CN105378160B (zh) * 2013-08-12 2018-04-17 伯乐生命医学产品有限公司 具有碱基配对的寡聚体的扩增报告子
DK3052658T3 (da) * 2013-09-30 2021-06-07 Vesicode Ab Fremgangsmåder til profilering af molekylkomplekser ved anvendelse af proksimitetsafhængig stregkodning
WO2015086604A1 (en) * 2013-12-11 2015-06-18 Qiagen Gmbh Nucleic acid detection and quantification
US10195610B2 (en) 2014-03-10 2019-02-05 Click Diagnostics, Inc. Cartridge-based thermocycler
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
GB201411567D0 (en) 2014-06-30 2014-08-13 Epistem Ltd Quantification methods
WO2016040523A1 (en) * 2014-09-12 2016-03-17 Click Diagnostics, Inc. Multiplex optical detection
ES2896198T3 (es) 2014-10-10 2022-02-24 Univ Rutgers Cebadores y sondas de reacción en cadena de polimerasa para Mycobacterium tuberculosis
CN107208139B (zh) 2014-12-09 2020-11-10 Seegene株式会社 与靶核酸序列有关的信号的分辨
CA2971200A1 (en) 2014-12-19 2016-06-23 Brandeis University Mispriming prevention reagents
JP6778194B2 (ja) 2014-12-31 2020-10-28 ビスビュー メディカル，インコーポレイテッド 分子診断試験のためのデバイス及び方法
CN107636165A (zh) * 2015-01-12 2018-01-26 生命科技公司 用于核酸文库质量控制和定量的方法、系统和其组合物
KR102165931B1 (ko) * 2015-09-24 2020-10-14 주식회사 씨젠 타겟 분석물질의 존재 또는 부존재를 결정하기 위한 다중 데이터 세트 분석법
WO2017087942A1 (en) 2015-11-20 2017-05-26 Brandeis University Amplifying and detecting rna and dna sequences comprising high levels of intramolecular hybridization
WO2017197040A1 (en) 2016-05-11 2017-11-16 Click Diagnostics, Inc. Devices and methods for nucleic acid extraction
CN109923613B (zh) * 2016-06-02 2023-07-04 Seegene株式会社 利用信号变化量数据集的样品内的目标分析物质检测方法
CN110325652A (zh) 2016-06-29 2019-10-11 易捷仪器诊断股份有限公司 使用流动池检测分子的装置和方法
JP6983906B2 (ja) * 2017-04-11 2021-12-17 ニューゲン テクノロジーズ， インコーポレイテッド ライブラリーの定量および定性
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
US20190093155A1 (en) * 2017-05-25 2019-03-28 Roche Molecular Systems, Inc. Multiplex Nucleic Acid Amplification Assay
WO2019045532A2 (en) * 2017-08-31 2019-03-07 Seegene, Inc. EVALUATION OF COMPONENT PERFORMANCE USING A PAIR OF DIMER FORMER PRIMERS
JP7239568B2 (ja) 2017-11-09 2023-03-14 ビスビュー メディカル，インコーポレイテッド 携帯型分子診断デバイスおよび標的ウイルスの検出方法
CN108570495A (zh) * 2018-01-15 2018-09-25 新开源博畅（武汉）生物科技有限公司 一种hla高分辨基因位点的快速扩增诊断试剂盒及扩增方法
CN108624664B (zh) * 2018-01-16 2020-08-28 武汉菲思特生物科技有限公司 一种急性肝损伤的早期诊断标志物的快速扩增方法
CA3088250A1 (en) * 2018-01-22 2019-07-25 Luminex Corporation Methods and compositions for discrete melt analysis
US12454720B2 (en) 2018-04-17 2025-10-28 ChromaCode, Inc. Methods and systems for multiplex analysis
EP3781708B1 (en) * 2018-04-20 2025-01-15 Seegene, Inc. Method for detecting a plurality of target nucleic acid sequences in sample
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
MX2021002533A (es) 2018-09-03 2021-05-27 Visby Medical Inc Dispositivos y metodos para pruebas de susceptibilidad a antibioticos.
CN109321672B (zh) * 2018-10-30 2021-07-06 中国农业大学 核苷酸组合物、试剂盒及检测方法
AU2020265586A1 (en) 2019-04-28 2021-10-14 Visby Medical, Inc. Molecular diagnostic devices with digital detection capability and wireless connectivity
US20220259641A1 (en) * 2019-07-17 2022-08-18 The Henry M. Jackson Foundation For The Advancement Of Military Medicine, Inc. Amplification assay for the detection of anaplasma phagocytophilum
CN110568175A (zh) * 2019-09-06 2019-12-13 成都理工大学 一种基于核酸染料诱导纳米金快速聚集检测dna的方法
JP7697691B2 (ja) 2019-09-23 2025-06-24 ユニベルシテイト ゲント Ｓｔｒジェノタイピングのためのプローブおよび方法
EP4085149A4 (en) 2020-01-03 2024-03-06 Visby Medical, Inc. Devices and methods for antibiotic susceptibility testing
US20230242971A1 (en) * 2020-05-08 2023-08-03 Roche Sequencing Solutions, Inc. Removal of excess oligonucleotides from a reation mixture
CN111961717B (zh) * 2020-08-28 2023-08-01 南方医科大学 单管同时检测缺失型和非缺失型α-地中海贫血基因的荧光PCR试剂盒
CN111909990B (zh) * 2020-08-28 2023-11-28 亚能生物技术（深圳）有限公司 单管同时检测基因的缺失突变和点突变的荧光pcr检测方法
EP4388132A1 (en) * 2021-08-19 2024-06-26 Luminex Corporation Digital amplification assay analysis method
JPWO2023248310A1 (enExample) * 2022-06-20 2023-12-28

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002097132A2 (en) * 2001-05-25 2002-12-05 The Secretary Of State For Defence Nucleic acid detection method

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5066584A (en) * 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) * 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5756285A (en) * 1991-09-27 1998-05-26 Amersham Life Science, Inc. DNA cycle sequencing
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5716784A (en) * 1996-02-05 1998-02-10 The Perkin-Elmer Corporation Fluorescence detection assay for homogeneous PCR hybridization systems
CA2252048C (en) * 1996-04-12 2008-03-11 The Public Health Research Institute Of The City Of New York, Inc. Detection probes, kits and assays
DE69739357D1 (de) * 1996-06-04 2009-05-28 Univ Utah Res Found Überwachung der hybridisierung während pcr
US5935791A (en) * 1997-09-23 1999-08-10 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
GB9725197D0 (en) 1997-11-29 1998-01-28 Secr Defence Detection system
US6140054A (en) * 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
US6277607B1 (en) * 1999-05-24 2001-08-21 Sanjay Tyagi High specificity primers, amplification methods and kits
EP1210358A4 (en) 1999-08-13 2005-01-05 Univ Brandeis DETECTION OF NUCLEIC ACIDS
US6472156B1 (en) 1999-08-30 2002-10-29 The University Of Utah Homogeneous multiplex hybridization analysis by color and Tm
CA2387306C (en) * 1999-10-22 2010-04-27 The Public Health Research Institute Of The City Of New York, Inc. Assays for short sequence variants
US20030082550A1 (en) * 2000-09-08 2003-05-01 Hans-Ulrich Thomann Mutations of the cyclooxygenase-2 gene
US7267945B2 (en) * 2001-03-26 2007-09-11 Applera Corporation Methods of determining the presence of polynucleotides employing amplification
CA2449560A1 (en) * 2001-06-08 2002-12-19 Shanghai Mendel Dna Center Co., Ltd. Low-temperature cycle extension of dna with high priming specificity
US20030108906A1 (en) * 2001-07-27 2003-06-12 Brooksbank Robert Alan Identification and use of molecules implicated in pain
EP1444365A4 (en) 2001-10-25 2005-07-20 Gorilla Genomics Inc ASYMMETRIC PCR WITH NUCLEASE-FREE POLYMERASE OR NUCLEASERESISTENT MOLECULAR BEACONS
WO2003045233A1 (en) 2001-11-21 2003-06-05 Optiscan Biomedical Corporation Method and apparatus for improving the accuracy of alternative site analyte concentration measurements
US7198897B2 (en) 2001-12-19 2007-04-03 Brandeis University Late-PCR
JP2004024035A (ja) 2002-06-21 2004-01-29 Tosoh Corp 核酸の検出方法
EP1394271B1 (en) * 2002-09-02 2007-11-14 Toyo Boseki Kabushiki Kaisha Method for identifying nucleotide polymorphisms using resonance energy transfer
US7718783B2 (en) * 2002-11-22 2010-05-18 Kaplan Mark H ELF3 gene compositions and methods
US20040175704A1 (en) * 2003-03-06 2004-09-09 Stratagene Compositions and methods for polynucleotide sequence detection
CA2548205C (en) * 2003-12-03 2017-06-27 Abbott Laboratories Double stranded linear nucleic acid probe and uses thereof
EP1733056B1 (en) * 2004-03-31 2013-05-22 The General Hospital Corporation Method to determine responsiveness of cancer to epidermal growth factor receptor targeting treatments
RU2460804C2 (ru) * 2004-10-18 2012-09-10 Брандейс Юнивесити Способ гомогенной детекции по меньшей мере одного продукта одноцепочечной амплификации

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002097132A2 (en) * 2001-05-25 2002-12-05 The Secretary Of State For Defence Nucleic acid detection method

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