JPH0543348B2 - - Google Patents

Info

Publication number
JPH0543348B2
JPH0543348B2 JP58501746A JP50174683A JPH0543348B2 JP H0543348 B2 JPH0543348 B2 JP H0543348B2 JP 58501746 A JP58501746 A JP 58501746A JP 50174683 A JP50174683 A JP 50174683A JP H0543348 B2 JPH0543348 B2 JP H0543348B2
Authority
JP
Japan
Prior art keywords
yeast
plasmid
lactis
kluyveromyces
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP58501746A
Other languages
English (en)
Japanese (ja)
Other versions
JPS59501572A (ja
Inventor
Rutsuho Edenzu
Adorianusu Marinusu Redeboeru
Korunerisu Teodoru Beritsupusu
Den Berugu Yohanesu Aberu Ban
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Unilever NV
Original Assignee
Unilever NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=19839766&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=JPH0543348(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Unilever NV filed Critical Unilever NV
Publication of JPS59501572A publication Critical patent/JPS59501572A/ja
Publication of JPH0543348B2 publication Critical patent/JPH0543348B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/43—Sweetening agents, e.g. thaumatin, monellin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pyrane Compounds (AREA)
JP58501746A 1982-05-19 1983-05-19 クルイベロミセス属酵母およびその製造法 Granted JPS59501572A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL8202091 1982-05-19
NL8202091 1982-05-19

Publications (2)

Publication Number Publication Date
JPS59501572A JPS59501572A (ja) 1984-09-06
JPH0543348B2 true JPH0543348B2 (h) 1993-07-01

Family

ID=19839766

Family Applications (3)

Application Number Title Priority Date Filing Date
JP58501746A Granted JPS59501572A (ja) 1982-05-19 1983-05-19 クルイベロミセス属酵母およびその製造法
JP58501749A Expired - Lifetime JP2608540B2 (ja) 1982-05-19 1983-05-19 クルイベロミセス種のためのクローニング系
JP61041331A Expired - Lifetime JPH0685720B2 (ja) 1982-05-19 1986-02-26 ソーマチン物質の製造法

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP58501749A Expired - Lifetime JP2608540B2 (ja) 1982-05-19 1983-05-19 クルイベロミセス種のためのクローニング系
JP61041331A Expired - Lifetime JPH0685720B2 (ja) 1982-05-19 1986-02-26 ソーマチン物質の製造法

Country Status (20)

Country Link
US (2) US4657857A (h)
EP (2) EP0096430B2 (h)
JP (3) JPS59501572A (h)
AR (1) AR241796A1 (h)
AT (2) ATE42338T1 (h)
AU (1) AU571181B2 (h)
BR (1) BR8307525A (h)
CA (1) CA1224735A (h)
DE (2) DE3379665D1 (h)
DK (1) DK173699B1 (h)
ES (1) ES8402874A1 (h)
FI (1) FI81379C (h)
GR (1) GR78852B (h)
HU (1) HU204097B (h)
IE (1) IE55817B1 (h)
IL (1) IL68733A (h)
NO (1) NO172397C (h)
NZ (1) NZ204302A (h)
PT (1) PT76727B (h)
WO (2) WO1983004051A1 (h)

Families Citing this family (64)

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Publication number Priority date Publication date Assignee Title
JPS58146281A (ja) * 1981-10-19 1983-08-31 Suntory Ltd 酵母細胞の形質転換法
GR78852B (h) * 1982-05-19 1984-10-02 Gist Brocades Nv
US4943529A (en) 1982-05-19 1990-07-24 Gist-Brocades Nv Kluyveromyces as a host strain
JPH0716432B2 (ja) * 1982-10-20 1995-03-01 サントリー株式会社 酵母の高発現ベクタープラスミドを用いたペプチドの生産方法
CA1341116C (en) * 1983-02-22 2000-10-17 Rae Lyn Burke Yeast expression systems with vectors having gapdh or pyk promoters and synthesis or foreign protein
US4876197A (en) * 1983-02-22 1989-10-24 Chiron Corporation Eukaryotic regulatable transcription
DE3480006D1 (en) * 1983-05-19 1989-11-09 Unilever Nv Improvements in the expression of newly introduced genes in yeast cells
AU577334B2 (en) * 1983-10-11 1988-09-22 International Genetic Engineering, Inc. Synthetic thaumatin genes
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US5422267A (en) * 1984-05-22 1995-06-06 Robert R. Yocum Industrial yeast comprising an integrated glucoamylase gene
US6214577B1 (en) 1984-05-22 2001-04-10 Robert Rogers Yocum Yeast vectors conferring antibiotic resistance
US5741672A (en) * 1984-07-27 1998-04-21 Unilever Patent Holdings B.V. Expression and production of polypeptides using the promoters of the hansenula polymorpha MOX and DAS genes
US5240838A (en) * 1984-07-27 1993-08-31 Internationale Otrool Maatschappij "Octropa" B.V. Regulatory sequences of alcohol oxidase (MOX) and dihydroxyacetonesynthase (DAS) of Hansenula polymorpha
US4837148A (en) * 1984-10-30 1989-06-06 Phillips Petroleum Company Autonomous replication sequences for yeast strains of the genus pichia
US4855231A (en) * 1984-10-30 1989-08-08 Phillips Petroleum Company Regulatory region for heterologous gene expression in yeast
US4879231A (en) * 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
US5047340A (en) * 1985-06-05 1991-09-10 The University Of Kentucky Research Foundation LAC+ saccharomyces cerevisiae
CA1310923C (en) * 1985-11-13 1992-12-01 Joachim Ludwig Weickmann Dna encoding [asp --] and [lys -, asp --] thaumatin i
IT1203758B (it) * 1986-03-27 1989-02-23 Univ Roma Vettori di clonazione e di espressione di geni eterologhi in lieviti e lieviti trasformati con tali vettori
CA1339101C (en) * 1986-06-03 1997-07-29 Nicolaas Overbeeke Production of guar alpha-galactosidase and immunologically related alpha-galactosidases by host organisms transformed with recombinant dna methods
US5151354A (en) * 1986-08-21 1992-09-29 Cornelius Hollenberg Fermentation processes using amylolytic enzyme producing microorganisms
EP0260404B1 (en) * 1986-08-21 1995-05-31 VAN DEN BERG, Robert Amylolytic enzymes producing microorganisms constructed by recombinant DNA technology and their use for fermentation processes
EP0257115A1 (en) * 1986-08-21 1988-03-02 Heineken Technisch Beheer B.V. Amylolytic enzyme producing microorganisms, constructed by recombinant DNA technology, and their use in fermentation processes
US5882884A (en) * 1987-06-19 1999-03-16 Lucky Biotech Corporation Expression of proteinaceous sweeteners in yeast
CA1323850C (en) * 1987-07-28 1993-11-02 Johannes A. Van Den Berg Kluyveromyces as a host strain
FR2649991B2 (fr) 1988-08-05 1994-03-04 Rhone Poulenc Sante Utilisation de derives stables du plasmide pkd1 pour l'expression et la secretion de proteines heterologues dans les levures du genre kluyveromyces
US5221624A (en) * 1988-11-08 1993-06-22 International Genetic Engineering, Inc. DNA encoding (Lys46, Asp97, Asp113) and (Lys46, Asp.sup.137) thaumatin I polypeptides
US5204252A (en) * 1989-02-08 1993-04-20 Henkel Research Corporation Candida tropicalis transformation system
JP3364616B2 (ja) 1989-02-24 2003-01-08 モンサント テクノロジー エルエルシー 合成植物遺伝子と調製方法
US20010003849A1 (en) 1989-08-07 2001-06-14 Kenneth A. Barton Expression of genes in plants
US5264554A (en) * 1990-01-19 1993-11-23 The Blood Center Of Southeastern Wisconsin, Inc. Platelet cell adhesion molecule and variants thereof
FR2678636A1 (fr) * 1991-07-02 1993-01-08 Rhone Poulenc Rorer Sa Procede de production de proteines recombinantes et cellules hote utilisees.
FR2680518A1 (fr) * 1991-08-21 1993-02-26 Rhone Poulenc Rorer Sa Promoteur de levure et son utilisation.
ES2080689B1 (es) * 1994-04-21 1996-09-01 Urquima Sa Procedimiento de obtencion de un edulcorante natural proteico.
US6682924B1 (en) * 1995-05-05 2004-01-27 Novozymes A/S Protease variants and compositions
US5837517A (en) * 1995-05-05 1998-11-17 Novo Nordisk A/S Protease variants and compositions
GB9907461D0 (en) 1999-03-31 1999-05-26 King S College London Neurite regeneration
ATE398181T1 (de) * 1997-04-11 2008-07-15 Dsm Ip Assets Bv Hefezellen welche zumindest zwei kopien von einem erwünschten gens enthalt und integrierte in dem chromosomal genom auf mehr als einem nicht- ribosomaler rns kodierende bereich, ins besonders mit kluyveromyces
US6265186B1 (en) 1997-04-11 2001-07-24 Dsm N.V. Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces
ES2154995B1 (es) * 1998-11-20 2001-11-16 Consejo Superior Investigacion Levadura panadera recombinante capaz de asimilar lactosa.
US8372620B2 (en) 1998-12-23 2013-02-12 Dupont Nutrition Biosciences Aps Bacterial xylanases
PT1183385E (pt) 1999-05-21 2006-11-30 Cargill Dow Llc Métodos e materiais para a síntese de produtos orgânicos
DE19943383B4 (de) * 1999-09-10 2006-03-16 Tad Pharma Gmbh Regulatorische Sequenzen und Expressionskassetten für Hefen
AU2001277735A1 (en) * 2000-08-10 2002-02-25 Ono Pharmaceutical Co. Ltd. Glycerylaldehyde-3-phosphate dehydrogenase promoter inducing cell death
GB0020498D0 (en) 2000-08-18 2000-10-11 Sterix Ltd Compound
CA2430270A1 (en) 2000-11-22 2002-05-30 Cargill Dow Polymers, Llc Methods and materials for the synthesis of organic products
US7141410B2 (en) * 2000-11-22 2006-11-28 Natureworks Llc Methods and materials for the production of organic products in cells of Candida species
ATE266087T1 (de) 2000-12-07 2004-05-15 Dsm Ip Assets Bv Verfahren zur verhinderung oder verminderung der trübung in getränken
ATE475324T1 (de) 2000-12-07 2010-08-15 Dsm Ip Assets Bv Mit carboxy-terminal prolin peptide angereicherte proteinhydrolysate
GB0116453D0 (en) 2001-07-05 2001-08-29 Imp College Innovations Ltd Method
HK1080090B (zh) 2001-10-10 2012-12-14 诺和诺德公司 肽的重构和糖缀合的方法
US7517671B2 (en) 2002-02-28 2009-04-14 New England Biolabs, Inc. Methods and compositions for concentrating secreted recombinant protein
BR0311655A (pt) 2002-06-07 2005-03-15 Dsm Ip Assets Bv Método melhorado para a prevenção ou redução de turbidez em bebidas
TWI328612B (en) 2002-07-25 2010-08-11 Dsm Ip Assets Bv Genes and their encoded polypeptides involved in the biosynthetic pathway of vitamin b12, vectors and host cells comprising the genes, and process for producing vitamin b12
EP2338333B1 (en) 2003-04-09 2017-09-06 ratiopharm GmbH Glycopegylation methods and proteins/peptides produced by the methods
GB0403864D0 (en) 2004-02-20 2004-03-24 Ucl Ventures Modulator
AU2006316397C1 (en) 2005-11-28 2024-08-08 Dsm Ip Assets B.V. Enzyme preparations yielding a clean taste
GB0600406D0 (en) 2006-01-10 2006-02-15 Univ Bath Crystal
PL2113027T3 (pl) 2007-02-20 2014-11-28 Dsm Ip Assets Bv Nowa sialidaza
DK2126060T3 (en) 2007-03-22 2017-01-16 Dsm Ip Assets Bv Hitherto unknown lysyl oxidases
WO2011101339A2 (en) 2010-02-18 2011-08-25 Dsm Ip Assets B.V. Fermentation broth and filtration filter cake and uses thereof
WO2011105841A2 (ko) * 2010-02-24 2011-09-01 중앙대학교 산학협력단 브라제인 발현용 재조합 발현벡터 및 높은 단맛을 가지는 신규한 브라제인 다중 변이체
WO2012007446A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Use of a mutarotase in the production of dried powders
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BR8205954A (pt) 1981-10-14 1983-09-13 Unilever Nv Sequencia de dna,plasmidio recombinante,cultura bacteriana e microorganismos
GR78852B (h) * 1982-05-19 1984-10-02 Gist Brocades Nv

Also Published As

Publication number Publication date
FI81379B (fi) 1990-06-29
FI840149A0 (fi) 1984-01-16
IE831185L (en) 1983-11-19
EP0096430A1 (en) 1983-12-21
JP2608540B2 (ja) 1997-05-07
ES522556A0 (es) 1984-03-01
IE55817B1 (en) 1991-01-30
DK173699B1 (da) 2001-07-02
EP0096910B1 (en) 1989-04-19
NZ204302A (en) 1987-03-06
NO172397B (no) 1993-04-05
WO1983004050A1 (en) 1983-11-24
DK11384D0 (da) 1984-01-11
AU1553983A (en) 1983-12-02
FI81379C (fi) 1990-10-10
JPH0685720B2 (ja) 1994-11-02
AU571181B2 (en) 1988-04-14
WO1983004051A1 (en) 1983-11-24
AR241796A1 (es) 1992-12-30
BR8307525A (pt) 1984-08-21
JPS61275227A (ja) 1986-12-05
CA1224735A (en) 1987-07-28
NO172397C (no) 1993-07-14
JPS59500848A (ja) 1984-05-17
EP0096910A1 (en) 1983-12-28
HU204097B (en) 1991-11-28
FI840149L (fi) 1984-01-16
EP0096430B2 (en) 1998-04-01
IL68733A (en) 1989-01-31
ATE49421T1 (de) 1990-01-15
EP0096430B1 (en) 1990-01-10
JPS59501572A (ja) 1984-09-06
US4859596A (en) 1989-08-22
PT76727A (en) 1983-06-01
DE3381090D1 (de) 1990-02-15
US4657857A (en) 1987-04-14
GR78852B (h) 1984-10-02
PT76727B (en) 1985-12-27
ATE42338T1 (de) 1989-05-15
DK11384A (da) 1984-01-11
DE3379665D1 (en) 1989-05-24
ES8402874A1 (es) 1984-03-01
NO840133L (no) 1984-01-13

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