JPH0258917B2 - - Google Patents
Info
- Publication number
- JPH0258917B2 JPH0258917B2 JP59005512A JP551284A JPH0258917B2 JP H0258917 B2 JPH0258917 B2 JP H0258917B2 JP 59005512 A JP59005512 A JP 59005512A JP 551284 A JP551284 A JP 551284A JP H0258917 B2 JPH0258917 B2 JP H0258917B2
- Authority
- JP
- Japan
- Prior art keywords
- plasmid
- dna
- wild
- region
- type
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000013612 plasmid Substances 0.000 claims abstract description 311
- 108020004414 DNA Proteins 0.000 claims abstract description 139
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 86
- 241000589158 Agrobacterium Species 0.000 claims abstract description 81
- 238000012546 transfer Methods 0.000 claims abstract description 71
- 239000013599 cloning vector Substances 0.000 claims abstract description 69
- 206010028980 Neoplasm Diseases 0.000 claims abstract description 37
- 108090000623 proteins and genes Proteins 0.000 claims description 132
- 238000010367 cloning Methods 0.000 claims description 74
- 238000000034 method Methods 0.000 claims description 68
- 239000003550 marker Substances 0.000 claims description 28
- 239000013600 plasmid vector Substances 0.000 claims description 20
- 238000002360 preparation method Methods 0.000 claims description 7
- 230000001613 neoplastic effect Effects 0.000 claims description 4
- 230000001939 inductive effect Effects 0.000 abstract description 6
- 231100001222 nononcogenic Toxicity 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 130
- 241000196324 Embryophyta Species 0.000 description 108
- 239000013598 vector Substances 0.000 description 78
- 239000012634 fragment Substances 0.000 description 70
- 239000002609 medium Substances 0.000 description 68
- 210000001519 tissue Anatomy 0.000 description 30
- 241000588724 Escherichia coli Species 0.000 description 27
- LMKYZBGVKHTLTN-NKWVEPMBSA-N D-nopaline Chemical compound NC(=N)NCCC[C@@H](C(O)=O)N[C@@H](C(O)=O)CCC(O)=O LMKYZBGVKHTLTN-NKWVEPMBSA-N 0.000 description 25
- 238000010586 diagram Methods 0.000 description 25
- 230000008569 process Effects 0.000 description 23
- 210000001938 protoplast Anatomy 0.000 description 20
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 19
- 239000000203 mixture Substances 0.000 description 19
- 229960000723 ampicillin Drugs 0.000 description 17
- 108091008146 restriction endonucleases Proteins 0.000 description 16
- IMXSCCDUAFEIOE-UHFFFAOYSA-N D-Octopin Natural products OC(=O)C(C)NC(C(O)=O)CCCN=C(N)N IMXSCCDUAFEIOE-UHFFFAOYSA-N 0.000 description 15
- IMXSCCDUAFEIOE-RITPCOANSA-N D-octopine Chemical compound [O-]C(=O)[C@@H](C)[NH2+][C@H](C([O-])=O)CCCNC(N)=[NH2+] IMXSCCDUAFEIOE-RITPCOANSA-N 0.000 description 15
- 230000010354 integration Effects 0.000 description 15
- 230000009466 transformation Effects 0.000 description 15
- 244000061176 Nicotiana tabacum Species 0.000 description 14
- 230000006870 function Effects 0.000 description 14
- 230000002068 genetic effect Effects 0.000 description 14
- 102000053602 DNA Human genes 0.000 description 13
- 206010020649 Hyperkeratosis Diseases 0.000 description 13
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 13
- 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 13
- 238000002474 experimental method Methods 0.000 description 13
- 239000003242 anti bacterial agent Substances 0.000 description 12
- 230000015572 biosynthetic process Effects 0.000 description 12
- 229940088710 antibiotic agent Drugs 0.000 description 11
- 230000003115 biocidal effect Effects 0.000 description 11
- 229930027917 kanamycin Natural products 0.000 description 11
- 229960000318 kanamycin Drugs 0.000 description 11
- 229930182823 kanamycin A Natural products 0.000 description 11
- 108090000790 Enzymes Proteins 0.000 description 10
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 10
- 238000012360 testing method Methods 0.000 description 10
- 229920001817 Agar Polymers 0.000 description 9
- 108091026890 Coding region Proteins 0.000 description 9
- 102000004190 Enzymes Human genes 0.000 description 9
- 239000008272 agar Substances 0.000 description 9
- 150000001413 amino acids Chemical class 0.000 description 9
- 229940088598 enzyme Drugs 0.000 description 9
- 239000002773 nucleotide Substances 0.000 description 9
- 125000003729 nucleotide group Chemical group 0.000 description 9
- 238000013518 transcription Methods 0.000 description 9
- 230000035897 transcription Effects 0.000 description 9
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 8
- 108010066133 D-octopine dehydrogenase Proteins 0.000 description 8
- 239000004098 Tetracycline Substances 0.000 description 8
- 101150086864 apt gene Proteins 0.000 description 8
- 230000029087 digestion Effects 0.000 description 8
- 238000003780 insertion Methods 0.000 description 8
- 230000037431 insertion Effects 0.000 description 8
- 238000002955 isolation Methods 0.000 description 8
- 229920001184 polypeptide Polymers 0.000 description 8
- 108090000765 processed proteins & peptides Proteins 0.000 description 8
- 102000004196 processed proteins & peptides Human genes 0.000 description 8
- 238000003786 synthesis reaction Methods 0.000 description 8
- 229960002180 tetracycline Drugs 0.000 description 8
- 229930101283 tetracycline Natural products 0.000 description 8
- 235000019364 tetracycline Nutrition 0.000 description 8
- 150000003522 tetracyclines Chemical class 0.000 description 8
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 7
- 235000001014 amino acid Nutrition 0.000 description 7
- 238000003491 array Methods 0.000 description 7
- FPPNZSSZRUTDAP-UWFZAAFLSA-N carbenicillin Chemical group N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)C(C(O)=O)C1=CC=CC=C1 FPPNZSSZRUTDAP-UWFZAAFLSA-N 0.000 description 7
- 229960003669 carbenicillin Drugs 0.000 description 7
- 230000012010 growth Effects 0.000 description 7
- 230000001976 improved effect Effects 0.000 description 7
- 238000013519 translation Methods 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 6
- 229930193140 Neomycin Natural products 0.000 description 6
- 238000012217 deletion Methods 0.000 description 6
- 230000037430 deletion Effects 0.000 description 6
- 239000000499 gel Substances 0.000 description 6
- 239000005556 hormone Substances 0.000 description 6
- 229940088597 hormone Drugs 0.000 description 6
- 229960004927 neomycin Drugs 0.000 description 6
- 229920003023 plastic Polymers 0.000 description 6
- 239000004033 plastic Substances 0.000 description 6
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 6
- 238000006467 substitution reaction Methods 0.000 description 6
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 5
- 206010027476 Metastases Diseases 0.000 description 5
- 229930006000 Sucrose Natural products 0.000 description 5
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 5
- 238000010276 construction Methods 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 230000006801 homologous recombination Effects 0.000 description 5
- 238000002744 homologous recombination Methods 0.000 description 5
- 208000015181 infectious disease Diseases 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 230000009401 metastasis Effects 0.000 description 5
- 108010058731 nopaline synthase Proteins 0.000 description 5
- 230000006798 recombination Effects 0.000 description 5
- 238000005215 recombination Methods 0.000 description 5
- 230000000717 retained effect Effects 0.000 description 5
- JQXXHWHPUNPDRT-WLSIYKJHSA-N rifampicin Chemical compound O([C@](C1=O)(C)O/C=C/[C@@H]([C@H]([C@@H](OC(C)=O)[C@H](C)[C@H](O)[C@H](C)[C@@H](O)[C@@H](C)\C=C\C=C(C)/C(=O)NC=2C(O)=C3C([O-])=C4C)C)OC)C4=C1C3=C(O)C=2\C=N\N1CC[NH+](C)CC1 JQXXHWHPUNPDRT-WLSIYKJHSA-N 0.000 description 5
- 229960001225 rifampicin Drugs 0.000 description 5
- 229960000268 spectinomycin Drugs 0.000 description 5
- UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 5
- 239000005720 sucrose Substances 0.000 description 5
- 102000012410 DNA Ligases Human genes 0.000 description 4
- 108010061982 DNA Ligases Proteins 0.000 description 4
- 108010042407 Endonucleases Proteins 0.000 description 4
- 102000004533 Endonucleases Human genes 0.000 description 4
- ULGZDMOVFRHVEP-RWJQBGPGSA-N Erythromycin Chemical compound O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)C(=O)[C@H](C)C[C@@](C)(O)[C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 ULGZDMOVFRHVEP-RWJQBGPGSA-N 0.000 description 4
- 241001646716 Escherichia coli K-12 Species 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- GPRBEKHLDVQUJE-VINNURBNSA-N cefotaxime Chemical compound N([C@@H]1C(N2C(=C(COC(C)=O)CS[C@@H]21)C(O)=O)=O)C(=O)/C(=N/OC)C1=CSC(N)=N1 GPRBEKHLDVQUJE-VINNURBNSA-N 0.000 description 4
- 230000021615 conjugation Effects 0.000 description 4
- 230000004927 fusion Effects 0.000 description 4
- 238000010353 genetic engineering Methods 0.000 description 4
- 238000000338 in vitro Methods 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 108020004999 messenger RNA Proteins 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 235000000346 sugar Nutrition 0.000 description 4
- 239000000052 vinegar Substances 0.000 description 4
- 235000021419 vinegar Nutrition 0.000 description 4
- 108020004638 Circular DNA Proteins 0.000 description 3
- 108020004705 Codon Proteins 0.000 description 3
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 3
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 3
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 description 3
- 108090000854 Oxidoreductases Proteins 0.000 description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 3
- 108020004511 Recombinant DNA Proteins 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 229960004261 cefotaxime Drugs 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 229960005091 chloramphenicol Drugs 0.000 description 3
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 3
- 239000013611 chromosomal DNA Substances 0.000 description 3
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 3
- 239000004062 cytokinin Substances 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 238000001976 enzyme digestion Methods 0.000 description 3
- 229960003276 erythromycin Drugs 0.000 description 3
- 238000001727 in vivo Methods 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 description 3
- 229960001669 kinetin Drugs 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 230000008520 organization Effects 0.000 description 3
- 229910052698 phosphorus Inorganic materials 0.000 description 3
- 239000011574 phosphorus Substances 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 229960005322 streptomycin Drugs 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- HCWLJSDMOMMDRF-SZWOQXJISA-N 3-[(3s,6r)-2-oxo-6-[(1s,2r,3r)-1,2,3,4-tetrahydroxybutyl]morpholin-3-yl]propanamide Chemical compound NC(=O)CC[C@@H]1NC[C@H]([C@@H](O)[C@H](O)[C@H](O)CO)OC1=O HCWLJSDMOMMDRF-SZWOQXJISA-N 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 2
- ILQOASSPNGAIBC-UHFFFAOYSA-N Agropine Natural products OC(O)C(O)CC(O)C1CN2C(CCC2=O)C(=O)O1 ILQOASSPNGAIBC-UHFFFAOYSA-N 0.000 description 2
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 2
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 2
- 229930192334 Auxin Natural products 0.000 description 2
- 241000908115 Bolivar Species 0.000 description 2
- 108091027551 Cointegrate Proteins 0.000 description 2
- 244000000626 Daucus carota Species 0.000 description 2
- 235000002767 Daucus carota Nutrition 0.000 description 2
- 101100481527 Escherichia phage N15 gene 19 gene Proteins 0.000 description 2
- FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 2
- 102000003960 Ligases Human genes 0.000 description 2
- 108090000364 Ligases Proteins 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 101150005851 NOS gene Proteins 0.000 description 2
- 102000004316 Oxidoreductases Human genes 0.000 description 2
- 108091000080 Phosphotransferase Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 229940126575 aminoglycoside Drugs 0.000 description 2
- 239000002363 auxin Substances 0.000 description 2
- VJBCNMFKFZIXHC-UHFFFAOYSA-N azanium;2-(4-methyl-5-oxo-4-propan-2-yl-1h-imidazol-2-yl)quinoline-3-carboxylate Chemical compound N.N1C(=O)C(C(C)C)(C)N=C1C1=NC2=CC=CC=C2C=C1C(O)=O VJBCNMFKFZIXHC-UHFFFAOYSA-N 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 210000000349 chromosome Anatomy 0.000 description 2
- 210000000078 claw Anatomy 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 238000007667 floating Methods 0.000 description 2
- 238000012248 genetic selection Methods 0.000 description 2
- 239000003630 growth substance Substances 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000001965 increasing effect Effects 0.000 description 2
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- 238000005304 joining Methods 0.000 description 2
- 230000013011 mating Effects 0.000 description 2
- 230000021121 meiosis Effects 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 229960000485 methotrexate Drugs 0.000 description 2
- 230000004899 motility Effects 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 102000020233 phosphotransferase Human genes 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 230000002062 proliferating effect Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 230000008929 regeneration Effects 0.000 description 2
- 238000011069 regeneration method Methods 0.000 description 2
- 230000010076 replication Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000006152 selective media Substances 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 230000001568 sexual effect Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
- 230000007704 transition Effects 0.000 description 2
- 230000005945 translocation Effects 0.000 description 2
- 210000004881 tumor cell Anatomy 0.000 description 2
- 230000004614 tumor growth Effects 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- ZBFDAUIVDSSISP-UHFFFAOYSA-N 5-methoxy-2-[(4-methoxy-3,5-dimethyl-2-pyridinyl)methylsulfinyl]-1H-imidazo[4,5-b]pyridine Chemical compound N=1C2=NC(OC)=CC=C2NC=1S(=O)CC1=NC=C(C)C(OC)=C1C ZBFDAUIVDSSISP-UHFFFAOYSA-N 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 244000153158 Ammi visnaga Species 0.000 description 1
- 235000010585 Ammi visnaga Nutrition 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 241000282994 Cervidae Species 0.000 description 1
- 208000003322 Coinfection Diseases 0.000 description 1
- 206010011409 Cross infection Diseases 0.000 description 1
- 102000004594 DNA Polymerase I Human genes 0.000 description 1
- 108010017826 DNA Polymerase I Proteins 0.000 description 1
- 238000012270 DNA recombination Methods 0.000 description 1
- 230000004543 DNA replication Effects 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 241001131785 Escherichia coli HB101 Species 0.000 description 1
- 101100176848 Escherichia phage N15 gene 15 gene Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-N L-arginine Chemical compound OC(=O)[C@@H](N)CCCN=C(N)N ODKSFYDXXFIFQN-BYPYZUCNSA-N 0.000 description 1
- 229930064664 L-arginine Natural products 0.000 description 1
- 235000014852 L-arginine Nutrition 0.000 description 1
- VPRLICVDSGMIKO-UHFFFAOYSA-N Mannopine Natural products NC(=O)CCC(C(O)=O)NCC(O)C(O)C(O)C(O)CO VPRLICVDSGMIKO-UHFFFAOYSA-N 0.000 description 1
- 240000001140 Mimosa pudica Species 0.000 description 1
- 235000016462 Mimosa pudica Nutrition 0.000 description 1
- 241001024304 Mino Species 0.000 description 1
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 1
- 241000208125 Nicotiana Species 0.000 description 1
- 241000208133 Nicotiana plumbaginifolia Species 0.000 description 1
- 206010029803 Nosocomial infection Diseases 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 240000007377 Petunia x hybrida Species 0.000 description 1
- -1 Phosphoric acid diester Chemical class 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 108700001094 Plant Genes Proteins 0.000 description 1
- 101150035718 Pno1 gene Proteins 0.000 description 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 241000187747 Streptomyces Species 0.000 description 1
- 108700005078 Synthetic Genes Proteins 0.000 description 1
- 108020005038 Terminator Codon Proteins 0.000 description 1
- 208000024780 Urticaria Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 125000000218 acetic acid group Chemical group C(C)(=O)* 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 239000006687 ag medium Substances 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000011681 asexual reproduction Effects 0.000 description 1
- 238000013465 asexual reproduction Methods 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 244000000005 bacterial plant pathogen Species 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 101150058049 car gene Proteins 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 230000034303 cell budding Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 229940088530 claforan Drugs 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- 231100000433 cytotoxic Toxicity 0.000 description 1
- 230000001472 cytotoxic effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 229940075894 denatured ethanol Drugs 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- MNQDKWZEUULFPX-UHFFFAOYSA-M dithiazanine iodide Chemical compound [I-].S1C2=CC=CC=C2[N+](CC)=C1C=CC=CC=C1N(CC)C2=CC=CC=C2S1 MNQDKWZEUULFPX-UHFFFAOYSA-M 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000009585 enzyme analysis Methods 0.000 description 1
- 125000004185 ester group Chemical group 0.000 description 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 108010092809 exonuclease Bal 31 Proteins 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000012239 gene modification Methods 0.000 description 1
- 238000012252 genetic analysis Methods 0.000 description 1
- 230000005017 genetic modification Effects 0.000 description 1
- 235000013617 genetically modified food Nutrition 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 101150085823 hsdR gene Proteins 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 238000002789 length control Methods 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- VPRLICVDSGMIKO-SZWOQXJISA-N mannopine Chemical compound NC(=O)CC[C@@H](C(O)=O)NC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO VPRLICVDSGMIKO-SZWOQXJISA-N 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 239000006870 ms-medium Substances 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 238000009377 nuclear transmutation Methods 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 125000003835 nucleoside group Chemical group 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 229940127240 opiate Drugs 0.000 description 1
- 210000001152 parietal lobe Anatomy 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 150000002972 pentoses Chemical class 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-N phosphoric acid Substances OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 1
- JOHZPMXAZQZXHR-UHFFFAOYSA-N pipemidic acid Chemical compound N1=C2N(CC)C=C(C(O)=O)C(=O)C2=CN=C1N1CCNCC1 JOHZPMXAZQZXHR-UHFFFAOYSA-N 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 101150079601 recA gene Proteins 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000011218 segmentation Effects 0.000 description 1
- 230000014639 sexual reproduction Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 244000000000 soil microbiome Species 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- 230000008467 tissue growth Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 230000005026 transcription initiation Effects 0.000 description 1
- 238000011426 transformation method Methods 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 210000003934 vacuole Anatomy 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8205—Agrobacterium mediated transformation
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP83100255.5 | 1983-01-13 | ||
EP83100255 | 1983-01-13 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS59140885A JPS59140885A (ja) | 1984-08-13 |
JPH0258917B2 true JPH0258917B2 (de) | 1990-12-11 |
Family
ID=8190232
Family Applications (6)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP59005512A Granted JPS59140885A (ja) | 1983-01-13 | 1984-01-13 | 植物細胞ゲノムへの発現可能な遺伝子の導入法 |
JP62129585A Expired - Lifetime JP2769539B2 (ja) | 1983-01-13 | 1987-05-25 | 植物細胞ゲノムへの発現可能な遺伝子の導入法 |
JP63103749A Expired - Fee Related JP2726267B2 (ja) | 1983-01-13 | 1988-04-25 | 植物細胞ゲノムへの発現可能な遺伝子の導入法 |
JP2236922A Pending JPH03108478A (ja) | 1983-01-13 | 1990-09-05 | 植物細胞ゲノムへの発現可能な遺伝子の導入法 |
JP4261509A Pending JPH06105629A (ja) | 1983-01-13 | 1992-09-30 | 植物細胞ゲノムへの発現可能な遺伝子の導入法 |
JP2000186437A Pending JP2001029092A (ja) | 1983-01-13 | 2000-06-21 | 植物細胞ゲノムへの発現可能な遺伝子の導入法 |
Family Applications After (5)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP62129585A Expired - Lifetime JP2769539B2 (ja) | 1983-01-13 | 1987-05-25 | 植物細胞ゲノムへの発現可能な遺伝子の導入法 |
JP63103749A Expired - Fee Related JP2726267B2 (ja) | 1983-01-13 | 1988-04-25 | 植物細胞ゲノムへの発現可能な遺伝子の導入法 |
JP2236922A Pending JPH03108478A (ja) | 1983-01-13 | 1990-09-05 | 植物細胞ゲノムへの発現可能な遺伝子の導入法 |
JP4261509A Pending JPH06105629A (ja) | 1983-01-13 | 1992-09-30 | 植物細胞ゲノムへの発現可能な遺伝子の導入法 |
JP2000186437A Pending JP2001029092A (ja) | 1983-01-13 | 2000-06-21 | 植物細胞ゲノムへの発現可能な遺伝子の導入法 |
Country Status (7)
Country | Link |
---|---|
JP (6) | JPS59140885A (de) |
AT (2) | ATE282692T1 (de) |
AU (1) | AU546542B2 (de) |
CA (2) | CA1341524C (de) |
DE (3) | DE3382839D1 (de) |
DK (1) | DK173104B1 (de) |
IL (1) | IL70610A (de) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2154156A2 (de) | 2001-03-15 | 2010-02-17 | Sumitomo Chemical Company, Limited | Analyse von Agonistenaktivität und Antagonistenaktivität auf einen Cytokininrezeptor |
EP2453018A1 (de) | 2001-10-19 | 2012-05-16 | Sumitomo Chemical Company, Limited | Herbizid metabolisierendes Protein, Gen davon und dessen Verwendung |
Families Citing this family (43)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ATE282692T1 (de) * | 1983-01-13 | 2004-12-15 | Max Planck Gesellschaft | Nichtonkogenisches ti-plasmidvektorsystem und für das einschleusen expressionsfähiger gene in pflanzengenomen verwendbare rekombinante dna- moleküle |
JPS60500438A (ja) * | 1983-01-17 | 1985-04-04 | モンサント カンパニ− | 植物細胞を形質転換するためのプラスミド |
SU1582990A3 (ru) * | 1983-01-17 | 1990-07-30 | Монсанто Компани (Фирма) | Способ получени трасформированных клеток двудольных растений |
WO1984002913A1 (en) * | 1983-01-17 | 1984-08-02 | Monsanto Co | Chimeric genes suitable for expression in plant cells |
NL8300699A (nl) * | 1983-02-24 | 1984-09-17 | Univ Leiden | Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; werkwijze voor het produceren van agrobacterium tumefaciens bacterien; stabiele cointegraat plasmiden; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten. |
NZ207766A (en) * | 1983-04-15 | 1987-03-06 | Lubrizol Genetics Inc | Plant structural gene expression |
NZ209338A (en) * | 1983-09-14 | 1988-02-12 | Lubrizol Genetics Inc | Plasmid for the transformation of a plant cell |
US4658082A (en) * | 1984-07-25 | 1987-04-14 | Atlantic Richfield Company | Method for producing intact plants containing foreign DNA |
BR8600161A (pt) * | 1985-01-18 | 1986-09-23 | Plant Genetic Systems Nv | Gene quimerico,vetores de plasmidio hibrido,intermediario,processo para controlar insetos em agricultura ou horticultura,composicao inseticida,processo para transformar celulas de plantas para expressar uma toxina de polipeptideo produzida por bacillus thuringiensis,planta,semente de planta,cultura de celulas e plasmidio |
US5180873A (en) * | 1985-04-16 | 1993-01-19 | Dna Plant Technology Corporation | Transformation of plants to introduce closely linked markers |
EP0267159A3 (de) * | 1986-11-07 | 1990-05-02 | Ciba-Geigy Ag | Verfahren zur genetischen Modifikation monokotyler Pflanzen |
EP2077275A3 (de) | 1998-06-02 | 2009-08-12 | Nihon University | IgA-Nephropathie-bezogene DNA |
JP4623825B2 (ja) | 1999-12-16 | 2011-02-02 | 協和発酵バイオ株式会社 | 新規ポリヌクレオチド |
CA2785941C (en) | 2000-10-06 | 2017-01-10 | Kyowa Hakko Kirin Co., Ltd. | Antibody composition-producing cell |
JP4627601B2 (ja) * | 2001-02-28 | 2011-02-09 | 株式会社パイロットコーポレーション | 筆記具用ペン芯およびそれを用いた筆記具 |
AU2003296151A1 (en) | 2002-12-26 | 2004-07-22 | Kyowa Hakko Kogyo Co., Ltd. | Process for producing dipeptide |
WO2007037245A1 (ja) | 2005-09-29 | 2007-04-05 | Shionogi & Co., Ltd. | 血管新生抑制作用を有するポリペプチド |
JP5242382B2 (ja) | 2006-04-14 | 2013-07-24 | 株式会社医学生物学研究所 | エフェクター機能を有するポリペプチド変異体 |
EP2067857B1 (de) | 2006-09-25 | 2012-04-11 | Kyowa Hakko Bio Co., Ltd | Verfahren zur dipeptidherstellung |
JPWO2008090960A1 (ja) | 2007-01-24 | 2010-05-20 | 協和発酵キリン株式会社 | ガングリオシドgm2に特異的に結合する遺伝子組換え抗体組成物 |
CN101679966B (zh) | 2007-01-24 | 2014-03-12 | 协和发酵麒麟株式会社 | 具有增强的效应子活性的遗传重组抗体组合物 |
US20100075343A1 (en) | 2007-01-25 | 2010-03-25 | Motoo Yamasaki | Novel peptides |
SG192433A1 (en) | 2007-09-27 | 2013-08-30 | Shionogi & Co | Method for producing hydroxylated adamantane using cytochrome p450 |
JP5620106B2 (ja) | 2007-10-24 | 2014-11-05 | 株式会社糖鎖工学研究所 | 増強されたエフェクター機能を有するポリペプチド |
JP5662149B2 (ja) | 2008-08-13 | 2015-01-28 | 協和発酵キリン株式会社 | 遺伝子組換えプロテインs組成物 |
CN102232111B (zh) | 2008-10-09 | 2014-12-03 | 协和梅迪克斯株式会社 | 果糖基肽氧化酶 |
EP2543727B1 (de) | 2010-03-02 | 2016-08-31 | Kyowa Hakko Kirin Co., Ltd. | Modifizierte antikörperzusammensetzung |
EP2554669B1 (de) | 2010-03-26 | 2018-09-19 | Kyowa Hakko Kirin Co., Ltd. | Neuer antikörper mit darin eingeführter modifikationsstelle und antikörperfragment |
JP5058332B2 (ja) | 2010-07-14 | 2012-10-24 | 住友ゴム工業株式会社 | イソプレンオリゴマー、ポリイソプレン、及びこれらの製造方法、ゴム組成物、並びに空気入りタイヤ |
US9944970B2 (en) | 2013-07-09 | 2018-04-17 | Kyowa Medex Co., Ltd. | Glycated hexapeptide oxidase and use thereof |
JP6448194B2 (ja) | 2014-01-20 | 2019-01-09 | 住友ゴム工業株式会社 | シス型プレニルトランスフェラーゼをコードする遺伝子、及び、NogoBreceptorをコードする遺伝子を宿主に導入することにより、該宿主において、シス型プレニルトランスフェラーゼ及びNogoBreceptorを発現させた形質転換体、並びに該形質転換体を用いたポリイソプレノイドの製造方法 |
JP6557989B2 (ja) | 2015-02-23 | 2019-08-14 | 住友ゴム工業株式会社 | 特定のプロモーター及び特定の蛋白質をコードする遺伝子を含むベクター、該ベクターが導入された形質転換植物、並びに、該ベクターを植物に導入することにより、ポリイソプレノイドの生産量を向上させる方法 |
JP6719174B2 (ja) | 2015-02-23 | 2020-07-08 | 住友ゴム工業株式会社 | 特定のプロモーター及び特定の蛋白質をコードする遺伝子を含むベクター、該ベクターが導入された形質転換植物、並びに、該ベクターを植物に導入することにより、ポリイソプレノイドの生産量を向上させる方法 |
JP6557990B2 (ja) | 2015-02-23 | 2019-08-14 | 住友ゴム工業株式会社 | 特定のプロモーター及び特定の蛋白質をコードする遺伝子を含むベクター、該ベクターが導入された形質転換植物、並びに、該ベクターを植物に導入することにより、ポリイソプレノイドの生産量を向上させる方法 |
TWI831365B (zh) | 2017-01-10 | 2024-02-01 | 國立大學法人山口大學 | 抗gpc3抗體 |
JP2020195326A (ja) | 2019-06-03 | 2020-12-10 | 住友ゴム工業株式会社 | 天然ゴムの製造方法、形質転換植物、空気入りタイヤの製造方法及びゴム製品の製造方法 |
JP6923166B2 (ja) | 2019-07-16 | 2021-08-18 | 住友ゴム工業株式会社 | 融合タンパク質、物質製造方法、ベクター、形質転換細胞、空気入りタイヤの製造方法及びゴム製品の製造方法 |
JP2021080204A (ja) | 2019-11-19 | 2021-05-27 | 住友ゴム工業株式会社 | 融合タンパク質、物質製造方法、ベクター、形質転換細胞、空気入りタイヤの製造方法及びゴム製品の製造方法 |
WO2021177074A1 (ja) | 2020-03-05 | 2021-09-10 | 住友ゴム工業株式会社 | ポリイソプレノイドの製造方法、ベクター、形質転換植物、空気入りタイヤの製造方法及びゴム製品の製造方法 |
JP2023040705A (ja) | 2021-09-10 | 2023-03-23 | 住友ゴム工業株式会社 | トランス型ポリイソプレノイドの製造方法、ベクター、形質転換生物、空気入りタイヤの製造方法及びゴム製品の製造方法 |
JP2023127870A (ja) | 2022-03-02 | 2023-09-14 | 住友ゴム工業株式会社 | 変異シス型プレニルトランスフェラーゼ(cpt)ファミリー蛋白質、ポリイソプレノイドの製造方法、ベクター、形質転換植物、空気入りタイヤの製造方法及びゴム製品の製造方法 |
JP2023127868A (ja) | 2022-03-02 | 2023-09-14 | 住友ゴム工業株式会社 | 変異シス型プレニルトランスフェラーゼ(cpt)ファミリー蛋白質、ポリイソプレノイドの製造方法、ベクター、形質転換植物、空気入りタイヤの製造方法及びゴム製品の製造方法 |
JP2023127869A (ja) | 2022-03-02 | 2023-09-14 | 住友ゴム工業株式会社 | 変異シス型プレニルトランスフェラーゼ(cpt)ファミリー蛋白質、ポリイソプレノイドの製造方法、ベクター、形質転換植物、空気入りタイヤの製造方法及びゴム製品の製造方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS54163600A (en) * | 1977-11-08 | 1979-12-26 | Genentech Inc | Microbiological polypeptide expression method and means |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS595512A (ja) * | 1982-06-30 | 1984-01-12 | 松下電工株式会社 | 接点材料 |
ATE282692T1 (de) * | 1983-01-13 | 2004-12-15 | Max Planck Gesellschaft | Nichtonkogenisches ti-plasmidvektorsystem und für das einschleusen expressionsfähiger gene in pflanzengenomen verwendbare rekombinante dna- moleküle |
JPS62129585A (ja) * | 1985-11-29 | 1987-06-11 | Kyocera Corp | ポンプ運転装置 |
-
1983
- 1983-12-22 AT AT89102441T patent/ATE282692T1/de not_active IP Right Cessation
- 1983-12-22 DE DE3382839T patent/DE3382839D1/de not_active Expired - Lifetime
- 1983-12-22 DE DE3382838T patent/DE3382838T2/de not_active Expired - Lifetime
- 1983-12-22 AT AT88105808T patent/ATE255162T1/de not_active IP Right Cessation
- 1983-12-22 DE DE8383112985T patent/DE3381568D1/de not_active Expired - Lifetime
-
1984
- 1984-01-03 IL IL70610A patent/IL70610A/xx not_active IP Right Cessation
- 1984-01-11 CA CA004451015A patent/CA1341524C/en not_active Expired - Lifetime
- 1984-01-11 CA CA000617136A patent/CA1341419C/en not_active Expired - Lifetime
- 1984-01-12 DK DK198400136A patent/DK173104B1/da not_active IP Right Cessation
- 1984-01-13 JP JP59005512A patent/JPS59140885A/ja active Granted
- 1984-01-13 AU AU23274/84A patent/AU546542B2/en not_active Expired
-
1987
- 1987-05-25 JP JP62129585A patent/JP2769539B2/ja not_active Expired - Lifetime
-
1988
- 1988-04-25 JP JP63103749A patent/JP2726267B2/ja not_active Expired - Fee Related
-
1990
- 1990-09-05 JP JP2236922A patent/JPH03108478A/ja active Pending
-
1992
- 1992-09-30 JP JP4261509A patent/JPH06105629A/ja active Pending
-
2000
- 2000-06-21 JP JP2000186437A patent/JP2001029092A/ja active Pending
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS54163600A (en) * | 1977-11-08 | 1979-12-26 | Genentech Inc | Microbiological polypeptide expression method and means |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2154156A2 (de) | 2001-03-15 | 2010-02-17 | Sumitomo Chemical Company, Limited | Analyse von Agonistenaktivität und Antagonistenaktivität auf einen Cytokininrezeptor |
EP2154155A2 (de) | 2001-03-15 | 2010-02-17 | Sumitomo Chemical Company, Limited | Analyse von Agonistenaktivität und Antagonistenaktivität auf einen Cytokininrezeptor |
EP2453018A1 (de) | 2001-10-19 | 2012-05-16 | Sumitomo Chemical Company, Limited | Herbizid metabolisierendes Protein, Gen davon und dessen Verwendung |
Also Published As
Publication number | Publication date |
---|---|
DK13684D0 (da) | 1984-01-12 |
DE3382838T2 (de) | 2004-04-15 |
IL70610A (en) | 1992-08-18 |
DE3382838D1 (de) | 2004-01-08 |
JPS63283587A (ja) | 1988-11-21 |
ATE282692T1 (de) | 2004-12-15 |
JPS59140885A (ja) | 1984-08-13 |
DK173104B1 (da) | 2000-01-17 |
JP2769539B2 (ja) | 1998-06-25 |
AU2327484A (en) | 1984-07-19 |
AU546542B2 (en) | 1985-09-05 |
DE3381568D1 (de) | 1990-06-21 |
CA1341419C (en) | 2003-02-11 |
JP2001029092A (ja) | 2001-02-06 |
DE3382839D1 (de) | 2004-12-23 |
JPH06105629A (ja) | 1994-04-19 |
ATE255162T1 (de) | 2003-12-15 |
JPH03108478A (ja) | 1991-05-08 |
CA1341524C (en) | 2007-04-03 |
DK13684A (da) | 1984-07-14 |
JP2726267B2 (ja) | 1998-03-11 |
JPS6339587A (ja) | 1988-02-20 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JPH0258917B2 (de) | ||
EP0320500B1 (de) | Nichtonkogenisches Ti-Plasmidvektorsystem und für das Einschleusen expressionsfähiger Gene in Pflanzengenomen verwendbare rekombinante DNA-Moleküle | |
US5668298A (en) | Selectable marker for development of vectors and transformation systems in plants | |
US5463174A (en) | Transformation and foreign gene expression in Brassica species | |
US5750871A (en) | Transformation and foreign gene expression in Brassica species | |
JP3102888B2 (ja) | 植物の形質転換方法及びそのためのベクター | |
JP3038479B2 (ja) | 植物のプロトプラストの形質転換法 | |
EP0270615B1 (de) | TRANSFORMATION UND EXPRESSION EINES FREMDEN GENS IN $i(BRASSICA) SPEZIES | |
US6365799B1 (en) | Hygromycin-resistant transgenic plants | |
WO2006004914A2 (en) | Biological gene transfer system for eukaryotic cells | |
TW319679B (de) | ||
Özyiğit | Agrobacterium tumefaciens and its use in plant biotechnology | |
JPH01160489A (ja) | 植物ゲノム内への遺伝子の指示された組込みを可能にする組換えdna分子 | |
JPH04502860A (ja) | 植物ゲノムに対するdnaの特定部位組込み法 | |
PT78415B (en) | Method for genetically modifying a plant cell | |
DK174310B1 (da) | Intermediær kloningsvektor, bakterie indeholdende denne, fremgangsmåde til transformering af en plantecelle, fremgangsmåde til fremstilling af en plante, transformeret plantecelle og plante af sådanne celler og frø af en sådan plante | |
Huang | Genetic engineering of Larix decidua: Agrobacterium-mediated gene transfer and regeneration of transgenic plants in European larch | |
Bond | Transformation as a tool to study the genetics of nodulation in Glycine max | |
Li et al. | Gene transfer strategy for kiwifruit to obtain pure transgenic plant through inducing adventitious roots in leaf explants with Agrobacterium tumefaciens |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
LAPS | Cancellation because of no payment of annual fees |