JP6560214B2 - プロテアーゼ開裂に対する感受性を低減した変異体α−アミラーゼ、及びその使用方法 - Google Patents
プロテアーゼ開裂に対する感受性を低減した変異体α−アミラーゼ、及びその使用方法 Download PDFInfo
- Publication number
- JP6560214B2 JP6560214B2 JP2016533121A JP2016533121A JP6560214B2 JP 6560214 B2 JP6560214 B2 JP 6560214B2 JP 2016533121 A JP2016533121 A JP 2016533121A JP 2016533121 A JP2016533121 A JP 2016533121A JP 6560214 B2 JP6560214 B2 JP 6560214B2
- Authority
- JP
- Japan
- Prior art keywords
- amylase
- amino acid
- enzyme
- starch
- pcuamy1
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01001—Alpha-amylase (3.2.1.1)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Enzymes And Modification Thereof (AREA)
- Detergent Compositions (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361906617P | 2013-11-20 | 2013-11-20 | |
| US61/906,617 | 2013-11-20 | ||
| PCT/US2014/065522 WO2015077126A1 (en) | 2013-11-20 | 2014-11-13 | Variant alpha-amylases having reduced susceptibility to protease cleavage, and methods of use, thereof |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016538860A JP2016538860A (ja) | 2016-12-15 |
| JP2016538860A5 JP2016538860A5 (enExample) | 2017-12-07 |
| JP6560214B2 true JP6560214B2 (ja) | 2019-08-14 |
Family
ID=52023630
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016533121A Expired - Fee Related JP6560214B2 (ja) | 2013-11-20 | 2014-11-13 | プロテアーゼ開裂に対する感受性を低減した変異体α−アミラーゼ、及びその使用方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20160272957A1 (enExample) |
| EP (1) | EP3071691B1 (enExample) |
| JP (1) | JP6560214B2 (enExample) |
| CN (1) | CN105960456A (enExample) |
| BR (1) | BR112016010551A2 (enExample) |
| MX (1) | MX2016006489A (enExample) |
| WO (1) | WO2015077126A1 (enExample) |
Families Citing this family (64)
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| WO2014099523A1 (en) * | 2012-12-21 | 2014-06-26 | Danisco Us Inc. | Alpha-amylase variants |
| EP3259358B1 (en) * | 2015-02-19 | 2024-06-19 | Danisco US Inc. | Enhanced protein expression |
| EP4219704A3 (en) | 2015-05-13 | 2023-08-23 | Danisco US Inc | Aprl-clade protease variants and uses thereof |
| WO2016201069A1 (en) | 2015-06-09 | 2016-12-15 | Danisco Us Inc | Low-density enzyme-containing particles |
| EP3307427B1 (en) | 2015-06-09 | 2023-08-16 | Danisco US Inc. | Osmotic burst encapsulates |
| WO2016201040A1 (en) | 2015-06-09 | 2016-12-15 | Danisco Us Inc. | Water-triggered enzyme suspension |
| EP3310911B1 (en) | 2015-06-17 | 2023-03-15 | Danisco US Inc. | Bacillus gibsonii-clade serine proteases |
| JP7364330B2 (ja) | 2015-11-05 | 2023-10-18 | ダニスコ・ユーエス・インク | パエニバチルス(Paenibacillus)属種及びバチルス(Bacillus)属種のマンナナーゼ |
| CN109072208A (zh) | 2015-11-05 | 2018-12-21 | 丹尼斯科美国公司 | 类芽孢杆菌属物种甘露聚糖酶 |
| WO2017100720A1 (en) * | 2015-12-09 | 2017-06-15 | Danisco Us Inc. | Alpha-amylase combinatorial variants |
| EP3390625B1 (en) | 2015-12-18 | 2023-09-06 | Danisco US Inc. | Polypeptides with endoglucanase activity and uses thereof |
| DE102016201643A1 (de) * | 2016-02-03 | 2017-08-03 | Henkel Ag & Co. Kgaa | Verbesserte Waschleistung durch eine alpha-Amylase aus Bacillus cereus |
| JP2019518440A (ja) | 2016-05-03 | 2019-07-04 | ダニスコ・ユーエス・インク | プロテアーゼ変異体およびその使用 |
| US20190136218A1 (en) | 2016-05-05 | 2019-05-09 | Danisco Us Inc | Protease variants and uses thereof |
| EP3455352A1 (en) * | 2016-05-09 | 2019-03-20 | Novozymes A/S | Variant polypeptides with improved performance and use of the same |
| US11661567B2 (en) | 2016-05-31 | 2023-05-30 | Danisco Us Inc. | Protease variants and uses thereof |
| CA3027745A1 (en) | 2016-06-17 | 2017-12-21 | Danisco Us Inc. | Protease variants and uses thereof |
| US20190264138A1 (en) | 2016-11-07 | 2019-08-29 | Danisco Us Inc. | Laundry detergent composition |
| WO2018118917A1 (en) | 2016-12-21 | 2018-06-28 | Danisco Us Inc. | Protease variants and uses thereof |
| CN110312794B (zh) | 2016-12-21 | 2024-04-12 | 丹尼斯科美国公司 | 吉氏芽孢杆菌进化枝丝氨酸蛋白酶 |
| WO2018169750A1 (en) | 2017-03-15 | 2018-09-20 | Danisco Us Inc | Trypsin-like serine proteases and uses thereof |
| US20200040283A1 (en) | 2017-03-31 | 2020-02-06 | Danisco Us Inc | Delayed release enzyme formulations for bleach-containing detergents |
| CN110662836B (zh) | 2017-03-31 | 2024-04-12 | 丹尼斯科美国公司 | α-淀粉酶组合变体 |
| JP7680825B2 (ja) | 2017-06-30 | 2025-05-21 | ダニスコ・ユーエス・インク | 低凝集の酵素含有粒子 |
| WO2019036721A2 (en) | 2017-08-18 | 2019-02-21 | Danisco Us Inc | VARIANTS OF ALPHA-AMYLASES |
| CN107653283B (zh) * | 2017-10-31 | 2021-02-09 | 新疆浙大阳光生物科技有限公司 | 一种黄霉素的制备方法 |
| WO2019108599A1 (en) | 2017-11-29 | 2019-06-06 | Danisco Us Inc | Subtilisin variants having improved stability |
| WO2019125683A1 (en) | 2017-12-21 | 2019-06-27 | Danisco Us Inc | Enzyme-containing, hot-melt granules comprising a thermotolerant desiccant |
| EP3749107A1 (en) | 2018-02-08 | 2020-12-16 | Danisco US Inc. | Thermally-resistant wax matrix particles for enzyme encapsulation |
| EP3799601A1 (en) | 2018-06-19 | 2021-04-07 | Danisco US Inc. | Subtilisin variants |
| EP3810767A1 (en) | 2018-06-19 | 2021-04-28 | Danisco US Inc. | Subtilisin variants |
| JP7530884B2 (ja) | 2018-07-31 | 2024-08-08 | ダニスコ・ユーエス・インク | 一般酸のpkaを低下させるアミノ酸置換を有する変異体アルファ-アミラーゼ |
| US20210189295A1 (en) | 2018-08-30 | 2021-06-24 | Danisco Us Inc | Enzyme-containing granules |
| EP3856882A1 (en) | 2018-09-27 | 2021-08-04 | Danisco US Inc. | Compositions for medical instrument cleaning |
| WO2020077331A2 (en) | 2018-10-12 | 2020-04-16 | Danisco Us Inc | Alpha-amylases with mutations that improve stability in the presence of chelants |
| US20230028935A1 (en) | 2018-11-28 | 2023-01-26 | Danisco Us Inc | Subtilisin variants having improved stability |
| WO2020242858A1 (en) | 2019-05-24 | 2020-12-03 | Danisco Us Inc | Subtilisin variants and methods of use |
| US20220306968A1 (en) | 2019-06-06 | 2022-09-29 | Danisco Us Inc | Methods and compositions for cleaning |
| US10941432B1 (en) * | 2019-08-15 | 2021-03-09 | Santiago De Urbina Gaviria | Method for the preparation of low molecular weight porcine lympho-reticular polypeptides |
| WO2021080948A2 (en) | 2019-10-24 | 2021-04-29 | Danisco Us Inc | Variant maltopentaose/maltohexaose-forming alpha-amylases |
| US20240034960A1 (en) | 2020-08-27 | 2024-02-01 | Danisco Us Inc | Enzymes and enzyme compositions for cleaning |
| US11926855B2 (en) | 2020-08-28 | 2024-03-12 | Kerry Group Services International Limited | Feed composition supplemented with a protease combination |
| JP7510309B2 (ja) * | 2020-08-31 | 2024-07-03 | 花王株式会社 | α-アミラーゼ変異体 |
| WO2022165107A1 (en) | 2021-01-29 | 2022-08-04 | Danisco Us Inc | Compositions for cleaning and methods related thereto |
| US20240294888A1 (en) | 2021-06-30 | 2024-09-05 | Danisco Us Inc. | Variant enzymes and uses thereof |
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| JP2023095355A (ja) * | 2021-12-24 | 2023-07-06 | 花王株式会社 | アミラーゼ配合洗浄剤組成物 |
| CN118974227A (zh) | 2022-03-01 | 2024-11-15 | 丹尼斯科美国公司 | 用于清洁的酶和酶组合物 |
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| EP4544016A1 (en) | 2022-06-21 | 2025-04-30 | Danisco US Inc. | Methods and compositions for cleaning comprising a polypeptide having thermolysin activity |
| CN119855892A (zh) | 2022-09-02 | 2025-04-18 | 丹尼斯科美国公司 | 洗涤剂组合物及其相关方法 |
| WO2024050339A1 (en) | 2022-09-02 | 2024-03-07 | Danisco Us Inc. | Mannanase variants and methods of use |
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| CN120677237A (zh) | 2023-02-01 | 2025-09-19 | 丹尼斯科美国公司 | 枯草杆菌蛋白酶变体和使用方法 |
| CN120712348A (zh) | 2023-03-06 | 2025-09-26 | 丹尼斯科美国公司 | 枯草杆菌蛋白酶变体和使用方法 |
| CN120813242A (zh) | 2023-03-16 | 2025-10-17 | 营养与生物科学美国第四公司 | 用于清洁和臭味控制的短芽孢杆菌发酵物提取物及其用途 |
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-
2014
- 2014-11-13 BR BR112016010551A patent/BR112016010551A2/pt not_active Application Discontinuation
- 2014-11-13 US US15/033,510 patent/US20160272957A1/en not_active Abandoned
- 2014-11-13 EP EP14812038.9A patent/EP3071691B1/en not_active Not-in-force
- 2014-11-13 MX MX2016006489A patent/MX2016006489A/es unknown
- 2014-11-13 JP JP2016533121A patent/JP6560214B2/ja not_active Expired - Fee Related
- 2014-11-13 WO PCT/US2014/065522 patent/WO2015077126A1/en not_active Ceased
- 2014-11-13 CN CN201480063251.2A patent/CN105960456A/zh active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CN105960456A (zh) | 2016-09-21 |
| US20160272957A1 (en) | 2016-09-22 |
| EP3071691B1 (en) | 2019-10-23 |
| MX2016006489A (es) | 2016-08-03 |
| JP2016538860A (ja) | 2016-12-15 |
| WO2015077126A1 (en) | 2015-05-28 |
| BR112016010551A2 (pt) | 2017-12-05 |
| EP3071691A1 (en) | 2016-09-28 |
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