JP5701894B2 - アッセイを行う方法及び装置 - Google Patents
アッセイを行う方法及び装置 Download PDFInfo
- Publication number
- JP5701894B2 JP5701894B2 JP2012540157A JP2012540157A JP5701894B2 JP 5701894 B2 JP5701894 B2 JP 5701894B2 JP 2012540157 A JP2012540157 A JP 2012540157A JP 2012540157 A JP2012540157 A JP 2012540157A JP 5701894 B2 JP5701894 B2 JP 5701894B2
- Authority
- JP
- Japan
- Prior art keywords
- assay
- microfluidic
- reaction vessel
- channel
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000003556 assay Methods 0.000 title claims description 91
- 238000000034 method Methods 0.000 title claims description 45
- 238000006243 chemical reaction Methods 0.000 claims description 153
- 239000000523 sample Substances 0.000 claims description 85
- 238000002955 isolation Methods 0.000 claims description 66
- 239000003153 chemical reaction reagent Substances 0.000 claims description 53
- 238000001514 detection method Methods 0.000 claims description 39
- 238000007836 assay cartridge Methods 0.000 claims description 34
- 238000004166 bioassay Methods 0.000 claims description 33
- 230000011664 signaling Effects 0.000 claims description 19
- 239000012491 analyte Substances 0.000 claims description 18
- 239000011521 glass Substances 0.000 claims description 17
- 239000000243 solution Substances 0.000 claims description 16
- 239000000872 buffer Substances 0.000 claims description 15
- 239000000758 substrate Substances 0.000 claims description 15
- 239000000126 substance Substances 0.000 claims description 14
- 239000000463 material Substances 0.000 claims description 13
- 102000004169 proteins and genes Human genes 0.000 claims description 13
- 108090000623 proteins and genes Proteins 0.000 claims description 13
- 238000005406 washing Methods 0.000 claims description 13
- 239000013610 patient sample Substances 0.000 claims description 9
- 102000004190 Enzymes Human genes 0.000 claims description 8
- 108090000790 Enzymes Proteins 0.000 claims description 8
- 230000027455 binding Effects 0.000 claims description 6
- 210000004369 blood Anatomy 0.000 claims description 5
- 239000008280 blood Substances 0.000 claims description 5
- 239000007853 buffer solution Substances 0.000 claims description 5
- 210000001175 cerebrospinal fluid Anatomy 0.000 claims description 5
- 230000005284 excitation Effects 0.000 claims description 5
- 230000001678 irradiating effect Effects 0.000 claims description 5
- 238000011068 loading method Methods 0.000 claims description 5
- 210000002381 plasma Anatomy 0.000 claims description 5
- 230000004044 response Effects 0.000 claims description 5
- 210000002966 serum Anatomy 0.000 claims description 5
- 210000002700 urine Anatomy 0.000 claims description 5
- 238000010256 biochemical assay Methods 0.000 claims description 4
- 230000000717 retained effect Effects 0.000 claims description 3
- 230000009870 specific binding Effects 0.000 claims description 3
- 238000010923 batch production Methods 0.000 claims description 2
- 238000007837 multiplex assay Methods 0.000 claims description 2
- 239000012530 fluid Substances 0.000 description 15
- 239000002699 waste material Substances 0.000 description 14
- 230000008569 process Effects 0.000 description 11
- 238000010586 diagram Methods 0.000 description 9
- 238000005516 engineering process Methods 0.000 description 8
- 239000004205 dimethyl polysiloxane Substances 0.000 description 7
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 7
- 238000002965 ELISA Methods 0.000 description 6
- 238000004590 computer program Methods 0.000 description 6
- 238000013461 design Methods 0.000 description 6
- 238000011049 filling Methods 0.000 description 6
- 239000013642 negative control Substances 0.000 description 6
- 239000002245 particle Substances 0.000 description 6
- 239000013641 positive control Substances 0.000 description 6
- 238000005086 pumping Methods 0.000 description 6
- 238000000926 separation method Methods 0.000 description 6
- 230000002411 adverse Effects 0.000 description 5
- 239000000427 antigen Substances 0.000 description 5
- 102000036639 antigens Human genes 0.000 description 5
- 108091007433 antigens Proteins 0.000 description 5
- 230000008901 benefit Effects 0.000 description 5
- 230000009260 cross reactivity Effects 0.000 description 5
- 230000006870 function Effects 0.000 description 5
- 239000004593 Epoxy Substances 0.000 description 4
- 230000037029 cross reaction Effects 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 238000007792 addition Methods 0.000 description 3
- 230000014759 maintenance of location Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000011159 matrix material Substances 0.000 description 3
- 239000013307 optical fiber Substances 0.000 description 3
- 238000003118 sandwich ELISA Methods 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 241000582786 Monoplex Species 0.000 description 2
- 238000001723 curing Methods 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 231100000673 dose–response relationship Toxicity 0.000 description 2
- 238000003018 immunoassay Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004020 luminiscence type Methods 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 230000009919 sequestration Effects 0.000 description 2
- 230000019491 signal transduction Effects 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000012131 assay buffer Substances 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 239000010432 diamond Substances 0.000 description 1
- 229910003460 diamond Inorganic materials 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 125000003700 epoxy group Chemical group 0.000 description 1
- 238000005530 etching Methods 0.000 description 1
- 238000002073 fluorescence micrograph Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002991 molded plastic Substances 0.000 description 1
- 150000003961 organosilicon compounds Chemical class 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 238000010587 phase diagram Methods 0.000 description 1
- -1 polydimethylsiloxane Polymers 0.000 description 1
- 229920000647 polyepoxide Polymers 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000002731 protein assay Methods 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- 239000004945 silicone rubber Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
- B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1095—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers
- G01N35/1097—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers characterised by the valves
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0636—Integrated biosensor, microarrays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0633—Valves, specific forms thereof with moving parts
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/52—Containers specially adapted for storing or dispensing a reagent
- B01L3/527—Containers specially adapted for storing or dispensing a reagent for a plurality of reagents
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Dispersion Chemistry (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US26357209P | 2009-11-23 | 2009-11-23 | |
| US61/263,572 | 2009-11-23 | ||
| PCT/US2010/057860 WO2011063408A1 (en) | 2009-11-23 | 2010-11-23 | Method and apparatus for performing assays |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015029544A Division JP5996014B2 (ja) | 2009-11-23 | 2015-02-18 | アッセイを行う方法及び装置 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013511731A JP2013511731A (ja) | 2013-04-04 |
| JP2013511731A5 JP2013511731A5 (enExample) | 2013-05-16 |
| JP5701894B2 true JP5701894B2 (ja) | 2015-04-15 |
Family
ID=44060083
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012540157A Active JP5701894B2 (ja) | 2009-11-23 | 2010-11-23 | アッセイを行う方法及び装置 |
| JP2015029544A Active JP5996014B2 (ja) | 2009-11-23 | 2015-02-18 | アッセイを行う方法及び装置 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015029544A Active JP5996014B2 (ja) | 2009-11-23 | 2015-02-18 | アッセイを行う方法及び装置 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US9229001B2 (enExample) |
| EP (1) | EP2504701B1 (enExample) |
| JP (2) | JP5701894B2 (enExample) |
| CN (1) | CN102713621B (enExample) |
| CA (1) | CA2781556C (enExample) |
| ES (1) | ES2649559T3 (enExample) |
| WO (1) | WO2011063408A1 (enExample) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110143378A1 (en) * | 2009-11-12 | 2011-06-16 | CyVek LLC. | Microfluidic method and apparatus for high performance biological assays |
| WO2013142847A1 (en) * | 2012-03-22 | 2013-09-26 | Cyvek, Inc | Pdms membrane-confined nucleic acid and antibody/antigen-functionalized microlength tube capture elements, and systems employing them |
| US9651568B2 (en) | 2009-11-23 | 2017-05-16 | Cyvek, Inc. | Methods and systems for epi-fluorescent monitoring and scanning for microfluidic assays |
| WO2013134741A2 (en) * | 2012-03-08 | 2013-09-12 | Cyvek, Inc. | Methods and systems for manufacture of microarray assay systems, conducting microfluidic assays, and monitoring and scanning to obtain microfluidic assay results |
| US9855735B2 (en) | 2009-11-23 | 2018-01-02 | Cyvek, Inc. | Portable microfluidic assay devices and methods of manufacture and use |
| US10065403B2 (en) | 2009-11-23 | 2018-09-04 | Cyvek, Inc. | Microfluidic assay assemblies and methods of manufacture |
| US9500645B2 (en) | 2009-11-23 | 2016-11-22 | Cyvek, Inc. | Micro-tube particles for microfluidic assays and methods of manufacture |
| US9759718B2 (en) | 2009-11-23 | 2017-09-12 | Cyvek, Inc. | PDMS membrane-confined nucleic acid and antibody/antigen-functionalized microlength tube capture elements, and systems employing them, and methods of their use |
| US9700889B2 (en) | 2009-11-23 | 2017-07-11 | Cyvek, Inc. | Methods and systems for manufacture of microarray assay systems, conducting microfluidic assays, and monitoring and scanning to obtain microfluidic assay results |
| CN103649759B (zh) | 2011-03-22 | 2016-08-31 | 西维克公司 | 微流体装置以及制造方法和用途 |
| DE102012205171B3 (de) * | 2012-03-29 | 2013-09-12 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Integriertes Einweg-Chipkartuschensystem für mobile Multiparameteranalysen chemischer und/oder biologischer Substanzen |
| US9458488B2 (en) * | 2013-03-15 | 2016-10-04 | Nanomix, Inc. | Point of care sensor systems |
| WO2014150853A1 (en) * | 2013-03-15 | 2014-09-25 | Inanovate, Inc. | Analyte measurement using longitudinal assay |
| US20170113221A1 (en) * | 2014-06-11 | 2017-04-27 | Micronics, Inc. | Microfluidic cartridges and apparatus with integrated assay controls for analysis of nucleic acids |
| CN104568537A (zh) * | 2014-11-05 | 2015-04-29 | 华文蔚 | 一种处理生物微流体样本的方法 |
| EP3288681A1 (en) * | 2015-04-30 | 2018-03-07 | Orphidia Limited | Microfluidic valves and devices |
| US10228367B2 (en) | 2015-12-01 | 2019-03-12 | ProteinSimple | Segmented multi-use automated assay cartridge |
| US9933445B1 (en) | 2016-05-16 | 2018-04-03 | Hound Labs, Inc. | System and method for target substance identification |
| CA3035286A1 (en) * | 2016-10-07 | 2018-04-12 | Boehringer Ingelheim Vetmedica Gmbh | Method and analysis system for testing a sample |
| US20200245898A1 (en) * | 2019-01-31 | 2020-08-06 | Hound Labs, Inc. | Single-use Microfluidic Cartridge for Detection of Target Chemical Presence in Human Breath |
| BR112021017166A2 (pt) * | 2019-03-01 | 2021-11-09 | Vidya Holdings Ltd | Cartucho de ensaio para detectar um componente alvo em um fluido, e, método para fabricar um arranjo de cartuchos de ensaio |
| US11977086B2 (en) | 2019-03-21 | 2024-05-07 | Hound Labs, Inc. | Biomarker detection from breath samples |
| US11933731B1 (en) | 2020-05-13 | 2024-03-19 | Hound Labs, Inc. | Systems and methods using Surface-Enhanced Raman Spectroscopy for detecting tetrahydrocannabinol |
| US12392769B1 (en) | 2021-01-12 | 2025-08-19 | Hound Labs, Inc. | Ambient contamination in breath analyte detection and measurement |
| CN114345430B (zh) * | 2022-01-14 | 2022-10-28 | 北京工商大学 | 一种通过纸基微流控芯片同时检测多种抗生素残留的便携式装置 |
| US20250128256A1 (en) * | 2022-01-25 | 2025-04-24 | President And Fellows Of Harvard College | Microfluidic devices with autonomous directional valves |
Family Cites Families (269)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5622871A (en) | 1987-04-27 | 1997-04-22 | Unilever Patent Holdings B.V. | Capillary immunoassay and device therefor comprising mobilizable particulate labelled reagents |
| SE343949B (enExample) | 1966-06-02 | 1972-03-20 | Pharmacia Ab | |
| US3867517A (en) | 1971-12-21 | 1975-02-18 | Abbott Lab | Direct radioimmunoassay for antigens and their antibodies |
| US3939350A (en) | 1974-04-29 | 1976-02-17 | Board Of Trustees Of The Leland Stanford Junior University | Fluorescent immunoassay employing total reflection for activation |
| US3876376A (en) | 1974-05-09 | 1975-04-08 | American Cyanamid Co | Linear determination of hemolytic complement activity in undiluted serum |
| US4222744A (en) | 1978-09-27 | 1980-09-16 | Becton Dickinson & Company | Assay for ligands |
| US4254096A (en) | 1979-10-04 | 1981-03-03 | Bio-Rad Laboratories, Inc. | Reagent combination for solid phase immunofluorescent assay |
| US4517288A (en) | 1981-01-23 | 1985-05-14 | American Hospital Supply Corp. | Solid phase system for ligand assay |
| US4425438A (en) | 1981-03-13 | 1984-01-10 | Bauman David S | Assay method and device |
| US4368047A (en) | 1981-04-27 | 1983-01-11 | University Of Utah Research Foundation | Process for conducting fluorescence immunoassays without added labels and employing attenuated internal reflection |
| NZ201901A (en) | 1981-09-25 | 1986-11-12 | Commw Serum Lab Commission | An apparatus suitable for performing automated heterogeneous immunoassays in a plurality of samples |
| DE3226407C2 (de) | 1982-07-15 | 1985-05-15 | Volker Dr. 6900 Heidelberg Daniel | Mikro-Analyse-Kapillar-System |
| US4447546A (en) | 1982-08-23 | 1984-05-08 | Myron J. Block | Fluorescent immunoassay employing optical fiber in capillary tube |
| JPH0627742B2 (ja) | 1982-12-21 | 1994-04-13 | コムテツク リサ−チ ユニツト リミテツド | 検定方法及びそのための装置 |
| EP0149168B1 (en) | 1983-12-19 | 1991-04-24 | Daiichi Pure Chemicals Co. Ltd. | Immunoassay |
| US4581624A (en) | 1984-03-01 | 1986-04-08 | Allied Corporation | Microminiature semiconductor valve |
| JPH06105261B2 (ja) * | 1984-03-05 | 1994-12-21 | 株式会社東芝 | 濃度勾配測定装置 |
| US5004923A (en) | 1985-08-05 | 1991-04-02 | Biotrack, Inc. | Capillary flow device |
| US5164598A (en) | 1985-08-05 | 1992-11-17 | Biotrack | Capillary flow device |
| US4716121A (en) | 1985-09-09 | 1987-12-29 | Ord, Inc. | Fluorescent assays, including immunoassays, with feature of flowing sample |
| US4844869A (en) | 1985-09-09 | 1989-07-04 | Ord, Inc. | Immunoassay apparatus |
| US5500350A (en) | 1985-10-30 | 1996-03-19 | Celltech Limited | Binding assay device |
| US4820490A (en) | 1986-09-11 | 1989-04-11 | Miles Inc. | Device and method for chemical analysis of fluids with a reagent coated light source |
| US4717545A (en) | 1986-09-11 | 1988-01-05 | Miles Inc. | Device and method for chemical analysis of fluids with a reagent coated light source |
| US4797259A (en) | 1986-12-15 | 1989-01-10 | Pall Corporation | Well-type diagnostic plate device |
| US4923819A (en) | 1987-03-27 | 1990-05-08 | Chimerix Corporation | Time-resolved fluorescence immunoassay |
| US4857453A (en) | 1987-04-07 | 1989-08-15 | Syntex (U.S.A.) Inc. | Immunoassay device |
| DE3878820T2 (de) | 1987-04-29 | 1993-07-22 | Celltech Ltd | Vorrichtung fuer bindungstest. |
| US5009998A (en) | 1987-06-26 | 1991-04-23 | E. I. Du Pont De Nemours And Company | Method for performing heterogeneous immunoassay |
| US5041181A (en) | 1987-10-06 | 1991-08-20 | Integrated Fluidics Company | Method of bonding plastics |
| US4908116A (en) | 1989-06-01 | 1990-03-13 | The Board Of Trustees At The Leland Stanford Junior University | Capillary electrophoretic device employing structure permitting electrical contact through ionic movement |
| US5302349A (en) | 1989-06-13 | 1994-04-12 | Diatron Corporation | Transient-state luminescence assay apparatus |
| US6008057A (en) | 1989-08-25 | 1999-12-28 | Roche Diagnostics Corporation | Immunoassay system |
| US5183740A (en) | 1990-02-23 | 1993-02-02 | The United States Of America As Represented By The Secretary Of The Navy | Flow immunosensor method and apparatus |
| SE470347B (sv) | 1990-05-10 | 1994-01-31 | Pharmacia Lkb Biotech | Mikrostruktur för vätskeflödessystem och förfarande för tillverkning av ett sådant system |
| JPH06501099A (ja) | 1990-09-11 | 1994-01-27 | ヴォアヒーズ・テクノロジース・インコーポレーテッド | 被覆された毛管 |
| JP2523227Y2 (ja) | 1991-07-30 | 1997-01-22 | 株式会社堀場製作所 | 異物検査装置 |
| US5296375A (en) | 1992-05-01 | 1994-03-22 | Trustees Of The University Of Pennsylvania | Mesoscale sperm handling devices |
| US5637469A (en) | 1992-05-01 | 1997-06-10 | Trustees Of The University Of Pennsylvania | Methods and apparatus for the detection of an analyte utilizing mesoscale flow systems |
| US5304487A (en) | 1992-05-01 | 1994-04-19 | Trustees Of The University Of Pennsylvania | Fluid handling in mesoscale analytical devices |
| US5885527A (en) * | 1992-05-21 | 1999-03-23 | Biosite Diagnostics, Inc. | Diagnostic devices and apparatus for the controlled movement of reagents without membrances |
| JPH06108242A (ja) | 1992-09-25 | 1994-04-19 | Minolta Camera Co Ltd | 薄膜電極および薄膜製造装置 |
| US5508200A (en) | 1992-10-19 | 1996-04-16 | Tiffany; Thomas | Method and apparatus for conducting multiple chemical assays |
| US5534328A (en) | 1993-12-02 | 1996-07-09 | E. I. Du Pont De Nemours And Company | Integrated chemical processing apparatus and processes for the preparation thereof |
| US5493802A (en) | 1993-05-26 | 1996-02-27 | Simson; Anton K. | Scroll displaying device |
| US5837546A (en) | 1993-08-24 | 1998-11-17 | Metrika, Inc. | Electronic assay device and method |
| ATE224053T1 (de) | 1993-11-12 | 2002-09-15 | Inverness Medical Switzerland | Vorrichtung zum ablesen von teststreifen |
| US5976896A (en) | 1994-06-06 | 1999-11-02 | Idexx Laboratories, Inc. | Immunoassays in capillary tubes |
| US5624850A (en) | 1994-06-06 | 1997-04-29 | Idetek, Inc. | Immunoassays in capillaries |
| US5593290A (en) | 1994-12-22 | 1997-01-14 | Eastman Kodak Company | Micro dispensing positive displacement pump |
| US5856174A (en) | 1995-06-29 | 1999-01-05 | Affymetrix, Inc. | Integrated nucleic acid diagnostic device |
| US6068751A (en) | 1995-12-18 | 2000-05-30 | Neukermans; Armand P. | Microfluidic valve and integrated microfluidic system |
| US6541213B1 (en) | 1996-03-29 | 2003-04-01 | University Of Washington | Microscale diffusion immunoassay |
| US5788814A (en) | 1996-04-09 | 1998-08-04 | David Sarnoff Research Center | Chucks and methods for positioning multiple objects on a substrate |
| US5942443A (en) | 1996-06-28 | 1999-08-24 | Caliper Technologies Corporation | High throughput screening assay systems in microscale fluidic devices |
| US5885470A (en) | 1997-04-14 | 1999-03-23 | Caliper Technologies Corporation | Controlled fluid transport in microfabricated polymeric substrates |
| JPH09288089A (ja) | 1996-04-23 | 1997-11-04 | Hitachi Ltd | 毛細管電気泳動装置 |
| CA2253710A1 (en) | 1996-04-25 | 1997-10-30 | Spectrametrix Inc. | Analyte assay using particulate labels |
| US7341727B1 (en) | 1996-05-03 | 2008-03-11 | Emergent Product Development Gaithersburg Inc. | M. catarrhalis outer membrane protein-106 polypeptide, methods of eliciting an immune response comprising same |
| NZ333346A (en) | 1996-06-28 | 2000-03-27 | Caliper Techn Corp | High-throughput screening assay systems in microscale fluidic devices |
| US20030054376A1 (en) | 1997-07-07 | 2003-03-20 | Mullis Kary Banks | Dual bead assays using cleavable spacers and/or ligation to improve specificity and sensitivity including related methods and apparatus |
| DE19635646C1 (de) | 1996-09-03 | 1997-12-04 | Bruker Franzen Analytik Gmbh | Korrektur der Massenbestimmung mit MALDI-Flugzeitmassenspektrometern |
| EP0990142A4 (en) | 1996-12-31 | 2000-09-27 | Genometrix Genomics Inc | MULTIPLEXED MOLECULAR ANALYSIS METHOD AND DEVICE |
| US6507989B1 (en) | 1997-03-13 | 2003-01-21 | President And Fellows Of Harvard College | Self-assembly of mesoscale objects |
| US6391622B1 (en) | 1997-04-04 | 2002-05-21 | Caliper Technologies Corp. | Closed-loop biochemical analyzers |
| KR100351531B1 (ko) | 1997-04-25 | 2002-09-11 | 캘리퍼 테크놀로지스 코포레이션 | 기하형상이 개선된 채널을 채용하는 미소 유체 장치 |
| US6020209A (en) | 1997-04-28 | 2000-02-01 | The United States Of America As Represented By The Secretary Of The Navy | Microcapillary-based flow-through immunosensor and displacement immunoassay using the same |
| US5882465A (en) | 1997-06-18 | 1999-03-16 | Caliper Technologies Corp. | Method of manufacturing microfluidic devices |
| US5932799A (en) | 1997-07-21 | 1999-08-03 | Ysi Incorporated | Microfluidic analyzer module |
| US6073482A (en) | 1997-07-21 | 2000-06-13 | Ysi Incorporated | Fluid flow module |
| US6293012B1 (en) | 1997-07-21 | 2001-09-25 | Ysi Incorporated | Method of making a fluid flow module |
| US6082185A (en) | 1997-07-25 | 2000-07-04 | Research International, Inc. | Disposable fluidic circuit cards |
| US5876675A (en) | 1997-08-05 | 1999-03-02 | Caliper Technologies Corp. | Microfluidic devices and systems |
| JP3938982B2 (ja) | 1997-08-29 | 2007-06-27 | オリンパス株式会社 | Dnaキャピラリィ |
| US6103537A (en) | 1997-10-02 | 2000-08-15 | Aclara Biosciences, Inc. | Capillary assays involving separation of free and bound species |
| US5842787A (en) | 1997-10-09 | 1998-12-01 | Caliper Technologies Corporation | Microfluidic systems incorporating varied channel dimensions |
| US5965237A (en) | 1997-10-20 | 1999-10-12 | Novartis Ag | Microstructure device |
| US6143152A (en) | 1997-11-07 | 2000-11-07 | The Regents Of The University Of California | Microfabricated capillary array electrophoresis device and method |
| US6167910B1 (en) | 1998-01-20 | 2001-01-02 | Caliper Technologies Corp. | Multi-layer microfluidic devices |
| US6251343B1 (en) | 1998-02-24 | 2001-06-26 | Caliper Technologies Corp. | Microfluidic devices and systems incorporating cover layers |
| US6756019B1 (en) | 1998-02-24 | 2004-06-29 | Caliper Technologies Corp. | Microfluidic devices and systems incorporating cover layers |
| US7497994B2 (en) | 1998-02-24 | 2009-03-03 | Khushroo Gandhi | Microfluidic devices and systems incorporating cover layers |
| US6100541A (en) | 1998-02-24 | 2000-08-08 | Caliper Technologies Corporation | Microfluidic devices and systems incorporating integrated optical elements |
| US6719868B1 (en) | 1998-03-23 | 2004-04-13 | President And Fellows Of Harvard College | Methods for fabricating microfluidic structures |
| JPH11307604A (ja) | 1998-04-17 | 1999-11-05 | Toshiba Corp | プロセスモニタ方法及びプロセス装置 |
| US6787111B2 (en) | 1998-07-02 | 2004-09-07 | Amersham Biosciences (Sv) Corp. | Apparatus and method for filling and cleaning channels and inlet ports in microchips used for biological analysis |
| US6576478B1 (en) | 1998-07-14 | 2003-06-10 | Zyomyx, Inc. | Microdevices for high-throughput screening of biomolecules |
| US6908770B1 (en) | 1998-07-16 | 2005-06-21 | Board Of Regents, The University Of Texas System | Fluid based analysis of multiple analytes by a sensor array |
| US20020049694A1 (en) | 1998-07-27 | 2002-04-25 | J. Wallace Parce | Distributed database for analytical instruments |
| US7155344B1 (en) | 1998-07-27 | 2006-12-26 | Caliper Life Sciences, Inc. | Distributed database for analytical instruments |
| US6086740A (en) | 1998-10-29 | 2000-07-11 | Caliper Technologies Corp. | Multiplexed microfluidic devices and systems |
| US6497155B1 (en) | 1999-02-09 | 2002-12-24 | Pharmacopeia, Inc. | Article comprising a particle retrieval device |
| US6303343B1 (en) | 1999-04-06 | 2001-10-16 | Caliper Technologies Corp. | Inefficient fast PCR |
| US20050100943A1 (en) | 2000-04-11 | 2005-05-12 | Hideki Kambara | Method of producing probe arrays for biological materials using fine particles |
| US6908737B2 (en) | 1999-04-15 | 2005-06-21 | Vitra Bioscience, Inc. | Systems and methods of conducting multiplexed experiments |
| US20040200909A1 (en) | 1999-05-28 | 2004-10-14 | Cepheid | Apparatus and method for cell disruption |
| AU5291600A (en) | 1999-06-01 | 2000-12-18 | Caliper Technologies Corporation | Microscale assays and microfluidic devices for transporter, gradient induced, and binding activities |
| US6520753B1 (en) | 1999-06-04 | 2003-02-18 | California Institute Of Technology | Planar micropump |
| US6929030B2 (en) | 1999-06-28 | 2005-08-16 | California Institute Of Technology | Microfabricated elastomeric valve and pump systems |
| US7144616B1 (en) | 1999-06-28 | 2006-12-05 | California Institute Of Technology | Microfabricated elastomeric valve and pump systems |
| US6899137B2 (en) | 1999-06-28 | 2005-05-31 | California Institute Of Technology | Microfabricated elastomeric valve and pump systems |
| EP1065378B1 (en) | 1999-06-28 | 2002-04-03 | California Institute of Technology | Microfabricated elastomeric valve and pump systems |
| US6533914B1 (en) | 1999-07-08 | 2003-03-18 | Shaorong Liu | Microfabricated injector and capillary array assembly for high-resolution and high throughput separation |
| AU6366200A (en) * | 1999-07-27 | 2001-02-13 | Cellomics, Inc. | Miniaturized cell array methods and apparatus for cell-based screening |
| US6762059B2 (en) * | 1999-08-13 | 2004-07-13 | U.S. Genomics, Inc. | Methods and apparatuses for characterization of single polymers |
| AU6932300A (en) * | 1999-08-25 | 2001-03-19 | Caliper Technologies Corporation | Dilutions in high throughput systems with a single vacuum source |
| US6383748B1 (en) | 1999-09-14 | 2002-05-07 | Pamgene B.V. | Analytical test device with substrate having oriented through going channels and improved methods and apparatus for using same |
| US6361958B1 (en) | 1999-11-12 | 2002-03-26 | Motorola, Inc. | Biochannel assay for hybridization with biomaterial |
| US6875619B2 (en) | 1999-11-12 | 2005-04-05 | Motorola, Inc. | Microfluidic devices comprising biochannels |
| JP3441058B2 (ja) | 1999-12-03 | 2003-08-25 | 理化学研究所 | キャピラリーゲル電気泳動用マイクロチップおよびその製造方法 |
| WO2001045843A2 (en) | 1999-12-22 | 2001-06-28 | Gene Logic, Inc. | Flow-thru chip cartridge, chip holder, system and method thereof |
| CA2401782A1 (en) | 2000-01-31 | 2001-08-02 | John T. Mcdevitt | Portable sensor array system |
| AU776266C (en) | 2000-01-31 | 2006-01-05 | Diagnoswiss S.A. | Method for fabricating micro-structures with various surface properties in multilayer body by plasma etching |
| US7867763B2 (en) | 2004-01-25 | 2011-01-11 | Fluidigm Corporation | Integrated chip carriers with thermocycler interfaces and methods of using the same |
| US7351376B1 (en) | 2000-06-05 | 2008-04-01 | California Institute Of Technology | Integrated active flux microfluidic devices and methods |
| CA2415055A1 (en) | 2000-08-03 | 2002-02-14 | Caliper Technologies Corporation | Methods and devices for high throughput fluid delivery |
| US7189358B2 (en) | 2000-08-08 | 2007-03-13 | California Institute Of Technology | Integrated micropump analysis chip and method of making the same |
| US7998746B2 (en) | 2000-08-24 | 2011-08-16 | Robert Otillar | Systems and methods for localizing and analyzing samples on a bio-sensor chip |
| AU2001290879A1 (en) | 2000-09-15 | 2002-03-26 | California Institute Of Technology | Microfabricated crossflow devices and methods |
| US6994826B1 (en) | 2000-09-26 | 2006-02-07 | Sandia National Laboratories | Method and apparatus for controlling cross contamination of microfluid channels |
| JP2002122596A (ja) * | 2000-10-16 | 2002-04-26 | Mitsubishi Rayon Co Ltd | 生体関連物質検出用マイクロアレイ |
| US20050221385A1 (en) | 2000-11-07 | 2005-10-06 | Caliper Life Sciences, Inc. | Pressure based mobility shift assays |
| AU2002230524A1 (en) | 2000-11-16 | 2002-05-27 | California Institute Of Technology | Apparatus and methods for conducting assays and high throughput screening |
| GB0028647D0 (en) | 2000-11-24 | 2001-01-10 | Nextgen Sciences Ltd | Apparatus for chemical assays |
| JP4248238B2 (ja) | 2001-01-08 | 2009-04-02 | プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ | マイクロ流体システム用の弁およびポンプならびにマイクロ流体システムを作製するための方法 |
| US20060207877A1 (en) | 2001-01-30 | 2006-09-21 | Walter Schmidt | Microfluidic device with various surface properties fabricated in multilayer body by plasma etching |
| JP2002286717A (ja) * | 2001-03-23 | 2002-10-03 | Central Res Inst Of Electric Power Ind | 無蛍光担体およびこれを固相に用いた測定用セル、ならびに無蛍光担体を利用する濃度測定方法 |
| WO2002081729A2 (en) | 2001-04-06 | 2002-10-17 | California Institute Of Technology | Nucleic acid amplification utilizing microfluidic devices |
| JP2002340900A (ja) * | 2001-05-18 | 2002-11-27 | Mitsubishi Rayon Co Ltd | バイオセンサー、バイオセンサーアレイ及び検出方法 |
| US7318912B2 (en) | 2001-06-07 | 2008-01-15 | Nanostream, Inc. | Microfluidic systems and methods for combining discrete fluid volumes |
| US6729352B2 (en) | 2001-06-07 | 2004-05-04 | Nanostream, Inc. | Microfluidic synthesis devices and methods |
| GB0116384D0 (en) | 2001-07-04 | 2001-08-29 | Diagnoswiss Sa | Microfluidic chemical assay apparatus and method |
| US6881579B2 (en) | 2001-07-30 | 2005-04-19 | Agilent Technologies, Inc. | Sample processing apparatus and methods |
| WO2003031066A1 (en) | 2001-10-11 | 2003-04-17 | California Institute Of Technology | Devices utilizing self-assembled gel and method of manufacture |
| US6750661B2 (en) | 2001-11-13 | 2004-06-15 | Caliper Life Sciences, Inc. | Method and apparatus for controllably effecting samples using two signals |
| US7691333B2 (en) | 2001-11-30 | 2010-04-06 | Fluidigm Corporation | Microfluidic device and methods of using same |
| EP1461606A4 (en) | 2001-12-05 | 2005-06-29 | Univ Washington | MICROFLUIDIC DEVICE AND SURFACE DECORATION PROCESS FOR SOLID PHASE AFFINITY BINDING ASSAYS |
| US6532997B1 (en) | 2001-12-28 | 2003-03-18 | 3M Innovative Properties Company | Sample processing device with integral electrophoresis channels |
| US7622083B2 (en) | 2002-01-28 | 2009-11-24 | Biocal Technology, Inc. | Multi-capillary electrophoresis cartridge interface mechanism |
| WO2003078045A2 (en) | 2002-03-12 | 2003-09-25 | Syngenta Participations Ag | Microcapillary hybridization chamber |
| US7524462B2 (en) | 2002-03-29 | 2009-04-28 | Agilent Technologies, Inc. | Capillary flow for a heterogenous assay in a micro-channel environment |
| US8211657B2 (en) | 2002-04-29 | 2012-07-03 | The Board Of Trustees Of The University Of Arkansas | Capillary-column-based bioseparator/bioreactor with an optical/electrochemical detector for detection of microbial pathogens |
| US7125510B2 (en) | 2002-05-15 | 2006-10-24 | Zhili Huang | Microstructure fabrication and microsystem integration |
| EP2093565B1 (en) | 2002-05-22 | 2014-11-19 | Platypus Technologies, Inc. | Uses of devices and methods for cellular assays |
| AU2003240738A1 (en) | 2002-06-03 | 2003-12-19 | Pamgene Bv | Method for high-throughput integrated chemical and biochemical reactions |
| AU2003277853A1 (en) | 2002-06-24 | 2004-01-06 | Fluidigm Corporation | Recirculating fluidic network and methods for using the same |
| US20040101444A1 (en) | 2002-07-15 | 2004-05-27 | Xeotron Corporation | Apparatus and method for fluid delivery to a hybridization station |
| US7164533B2 (en) | 2003-01-22 | 2007-01-16 | Cyvera Corporation | Hybrid random bead/chip based microarray |
| AU2003270004A1 (en) | 2002-08-28 | 2004-03-19 | Mt Technologies, Inc. | Microfluidic affinity system using polydimethylsiloxane and a surface modification process |
| EP1540590A1 (en) | 2002-09-12 | 2005-06-15 | Cyvera Corporation | Assay stick comprising coded microbeads |
| US20100255603A9 (en) | 2002-09-12 | 2010-10-07 | Putnam Martin A | Method and apparatus for aligning microbeads in order to interrogate the same |
| CA2499046A1 (en) | 2002-09-12 | 2004-03-25 | Cyvera Corporation | Diffraction grating-based encoded micro-particles for multiplexed experiments |
| WO2004024328A1 (en) | 2002-09-12 | 2004-03-25 | Cyvera Corporation | Method and apparatus for aligning elongated microbeads in order to interrogate the same |
| JP2006501056A (ja) | 2002-09-25 | 2006-01-12 | カリフォルニア インスティテュート オブ テクノロジー | ミクロ流体大規模集積 |
| US7186383B2 (en) | 2002-09-27 | 2007-03-06 | Ast Management Inc. | Miniaturized fluid delivery and analysis system |
| TW590982B (en) | 2002-09-27 | 2004-06-11 | Agnitio Science & Technology I | Micro-fluid driving device |
| EP1560642A4 (en) | 2002-10-09 | 2006-05-03 | Univ Illinois | MICROFLUID SYSTEMS AND COMPONENTS |
| US7390457B2 (en) | 2002-10-31 | 2008-06-24 | Agilent Technologies, Inc. | Integrated microfluidic array device |
| WO2004059299A1 (en) | 2002-12-16 | 2004-07-15 | Cytodiscovery, Inc. | Microfluidic system with integrated permeable membrane |
| US20050266582A1 (en) | 2002-12-16 | 2005-12-01 | Modlin Douglas N | Microfluidic system with integrated permeable membrane |
| CA2521999A1 (en) | 2002-12-20 | 2004-09-02 | Biotrove, Inc. | Assay apparatus and method using microfluidic arrays |
| CN102620959B (zh) | 2002-12-26 | 2015-12-16 | 梅索磅秤技术有限公司 | 检定盒及其使用方法 |
| KR101216828B1 (ko) | 2002-12-30 | 2013-01-04 | 더 리전트 오브 더 유니버시티 오브 캘리포니아 | 병원균 검출과 분석을 위한 방법과 기구 |
| US7033476B2 (en) | 2002-12-31 | 2006-04-25 | Ut-Battelle, Llc | Separation and counting of single molecules through nanofluidics, programmable electrophoresis, and nanoelectrode-gated tunneling and dielectric detection |
| US7122153B2 (en) | 2003-01-08 | 2006-10-17 | Ho Winston Z | Self-contained microfluidic biochip and apparatus |
| US7476363B2 (en) | 2003-04-03 | 2009-01-13 | Fluidigm Corporation | Microfluidic devices and methods of using same |
| TW579430B (en) | 2003-05-02 | 2004-03-11 | Dr Chip Biotechnology Inc | Automatic micro-fluid hybridization chip platform |
| US7695683B2 (en) | 2003-05-20 | 2010-04-13 | Fluidigm Corporation | Method and system for microfluidic device and imaging thereof |
| US7238269B2 (en) | 2003-07-01 | 2007-07-03 | 3M Innovative Properties Company | Sample processing device with unvented channel |
| FR2857099B1 (fr) | 2003-07-04 | 2005-10-07 | Jean Marie Billiotte | Procede et dispositif d'analyse chimique ou biologique par senseur a chambre monolithique en gerbe multi-micro-tubulaire et transducteur lateral de mesure integrale |
| US7341841B2 (en) * | 2003-07-12 | 2008-03-11 | Accelr8 Technology Corporation | Rapid microbial detection and antimicrobial susceptibility testing |
| US7028536B2 (en) | 2004-06-29 | 2006-04-18 | Nanostream, Inc. | Sealing interface for microfluidic device |
| US20050098750A1 (en) | 2003-11-06 | 2005-05-12 | Daniel Sobek | Electrostatic sealing device and method of use thereof |
| JP4073023B2 (ja) | 2003-11-07 | 2008-04-09 | 財団法人新産業創造研究機構 | 微小流路デバイスおよびその作製方法 |
| US7943089B2 (en) | 2003-12-19 | 2011-05-17 | Kimberly-Clark Worldwide, Inc. | Laminated assay devices |
| CA2834041C (en) | 2003-12-31 | 2017-05-16 | President And Fellows Of Harvard College | Assay device and method |
| WO2005083390A1 (en) * | 2004-02-20 | 2005-09-09 | Research Foundation Of The State University Of New York | Method and device for manipulating liquids in microfluidic systems |
| EP3270156A1 (en) | 2004-04-07 | 2018-01-17 | Abbott Laboratories | Disposable chamber for analyzing biologic fluids |
| JP5502275B2 (ja) | 2004-05-02 | 2014-05-28 | フルイディグム コーポレイション | 熱反応デバイスおよびその熱反応デバイスの使用方法 |
| US7887750B2 (en) | 2004-05-05 | 2011-02-15 | Bayer Healthcare Llc | Analytical systems, devices, and cartridges therefor |
| US7419639B2 (en) | 2004-05-12 | 2008-09-02 | The Board Of Trustees Of The Leland Stanford Junior University | Multilayer microfluidic device |
| US7799553B2 (en) | 2004-06-01 | 2010-09-21 | The Regents Of The University Of California | Microfabricated integrated DNA analysis system |
| EP1776581B1 (en) | 2004-07-19 | 2015-05-06 | ProteinSimple | Method for protein detection |
| JP2006098169A (ja) * | 2004-09-29 | 2006-04-13 | Fujitsu Ltd | 被検体定量デバイスおよび被検体定量方法 |
| EP1794581A2 (en) | 2004-09-15 | 2007-06-13 | Microchip Biotechnologies, Inc. | Microfluidic devices |
| US7832429B2 (en) | 2004-10-13 | 2010-11-16 | Rheonix, Inc. | Microfluidic pump and valve structures and fabrication methods |
| CN101036056A (zh) | 2004-10-29 | 2007-09-12 | 伊藤火腿株式会社 | 反应容器 |
| JP2008522795A (ja) | 2004-12-03 | 2008-07-03 | カリフォルニア インスティチュート オブ テクノロジー | 化学反応回路を有するマイクロ流体装置 |
| US7682817B2 (en) | 2004-12-23 | 2010-03-23 | Kimberly-Clark Worldwide, Inc. | Microfluidic assay devices |
| US20060228734A1 (en) * | 2005-03-18 | 2006-10-12 | Applera Corporation | Fluid processing device with captured reagent beads |
| DE102005049365A1 (de) | 2005-03-18 | 2006-09-21 | BAM Bundesanstalt für Materialforschung und -prüfung | Kalibriereinrichtung und Farbstoffkit sowie ihre Verwendung zur Charakterisierung von Lumineszenzmesssystemen |
| CA2602493C (en) * | 2005-03-23 | 2015-03-17 | Velocys, Inc. | Surface features in microprocess technology |
| JP2008538609A (ja) | 2005-04-20 | 2008-10-30 | ベクトン・ディキンソン・アンド・カンパニー | マルチプレックスマイクロ粒子システム |
| CA2505657A1 (en) | 2005-04-28 | 2006-10-28 | York University | Method for mixing inside a capillary and device for achieving same |
| WO2006122311A2 (en) | 2005-05-11 | 2006-11-16 | The Trustees Of The University Of Pennsylvania | Microfluidic chip |
| JPWO2006123578A1 (ja) | 2005-05-19 | 2008-12-25 | コニカミノルタエムジー株式会社 | 検体中の標的物質を分析するための検査チップおよびマイクロ総合分析システム |
| EP1884766A4 (en) * | 2005-05-20 | 2013-10-16 | Hitachi Chemical Co Ltd | BIOCHEMICAL ANALYSIS PROCESS |
| US20060263818A1 (en) * | 2005-05-23 | 2006-11-23 | Axel Scherer | High throughput multi-antigen microfluidic fluorescence immunoassays |
| EP2477029A1 (en) | 2005-06-02 | 2012-07-18 | Fluidigm Corporation | Analysis using microfluidic partitioning devices |
| US20090142853A1 (en) | 2005-08-11 | 2009-06-04 | Eksigent Technologies, Llc | Microfluidic system and methods |
| RO122612B1 (ro) | 2005-08-29 | 2009-09-30 | Institutul Naţional De Cercetare-Dezvoltare Pentru Microtehnologie | Procedeu de realizare a unui biocip cu funcţia de amplificare a unor fragmente specifice de adn prin reacţia polimerazică în lan |
| US20070074972A1 (en) | 2005-09-13 | 2007-04-05 | Fluidigm Corporation | Microfluidic assay devices and methods |
| JP3116709U (ja) * | 2005-09-13 | 2005-12-15 | 有限会社メタボスクリーン | 微小流路チップ |
| US20080311585A1 (en) | 2005-11-02 | 2008-12-18 | Affymetrix, Inc. | System and method for multiplex liquid handling |
| US7763453B2 (en) | 2005-11-30 | 2010-07-27 | Micronics, Inc. | Microfluidic mixing and analytic apparatus |
| JP4253323B2 (ja) * | 2005-12-05 | 2009-04-08 | 株式会社東芝 | マイクロ化学チップ |
| US8182767B2 (en) | 2005-12-27 | 2012-05-22 | Honeywell International Inc. | Needle-septum interface for a fluidic analyzer |
| WO2007092713A2 (en) | 2006-02-02 | 2007-08-16 | Trustees Of The University Of Pennsylvania | Microfluidic system and method for analysis of gene expression in cell-containing samples and detection of disease |
| EP1979079A4 (en) | 2006-02-03 | 2012-11-28 | Integenx Inc | MICROFLUIDIC DEVICES |
| US8124015B2 (en) | 2006-02-03 | 2012-02-28 | Institute For Systems Biology | Multiplexed, microfluidic molecular assay device and assay method |
| EP1987275A1 (en) | 2006-02-13 | 2008-11-05 | Koninklijke Philips Electronics N.V. | Microfluidic device for molecular diagnostic applications |
| US7639026B2 (en) | 2006-02-24 | 2009-12-29 | Johnstech International Corporation | Electronic device test set and contact used therein |
| CN101067642A (zh) * | 2006-02-24 | 2007-11-07 | 丹尼斯·B·歇尔 | 电子器件测试装置及其所使用的触头 |
| WO2007106580A2 (en) * | 2006-03-15 | 2007-09-20 | Micronics, Inc. | Rapid magnetic flow assays |
| US7766033B2 (en) | 2006-03-22 | 2010-08-03 | The Regents Of The University Of California | Multiplexed latching valves for microfluidic devices and processors |
| US8741230B2 (en) | 2006-03-24 | 2014-06-03 | Theranos, Inc. | Systems and methods of sample processing and fluid control in a fluidic system |
| US8293524B2 (en) | 2006-03-31 | 2012-10-23 | Fluxion Biosciences Inc. | Methods and apparatus for the manipulation of particle suspensions and testing thereof |
| WO2007125468A2 (en) | 2006-05-01 | 2007-11-08 | Koninklijke Philips Electronics N.V. | Fluid sample transport device with reduced dead volume for processing, controlling and/or detecting a fluid sample |
| AU2007254191B2 (en) | 2006-05-16 | 2013-01-10 | Fluidigm Corporation | PCR-free sample preparation and detection systems for high speed biologic analysis and identification |
| EP2041573B1 (en) | 2006-06-23 | 2019-09-04 | PerkinElmer Health Sciences, Inc. | Methods and devices for microfluidic point-of-care immunoassays |
| CA2656686A1 (en) | 2006-07-05 | 2008-01-10 | Aida Engineering, Ltd. | Micro passage chip and fluid transferring method |
| US7794665B2 (en) * | 2006-07-17 | 2010-09-14 | Industrial Technology Research Institute | Fluidic device |
| US20080017512A1 (en) | 2006-07-24 | 2008-01-24 | Bordunov Andrei V | Coatings for capillaries capable of capturing analytes |
| GR1006447B (el) | 2006-09-15 | 2009-06-19 | Εθνικο Κεντρο Ερευνας Φυσικων Επιστημων (Εκεφε) "Δημοκριτος" | Μεθοδος συγκολλησης |
| WO2008147382A1 (en) | 2006-09-27 | 2008-12-04 | Micronics, Inc. | Integrated microfluidic assay devices and methods |
| US20080131327A1 (en) | 2006-09-28 | 2008-06-05 | California Institute Of Technology | System and method for interfacing with a microfluidic chip |
| EP2074341A4 (en) | 2006-10-04 | 2013-04-10 | Fluidigm Corp | MICROFLUIDIC CHECK VALVES |
| WO2008043041A1 (en) | 2006-10-04 | 2008-04-10 | University Of Washington | Method and device for rapid parallel microfluidic molecular affinity assays |
| EP2084104A1 (en) | 2006-11-03 | 2009-08-05 | McGill University | Electrical microvalve and method of manufacturing thereof |
| US8425863B2 (en) | 2006-12-19 | 2013-04-23 | Koninklijke Philips Electronics N.V. | Micro fluidic device |
| EP2125219B1 (en) | 2007-01-19 | 2016-08-10 | Fluidigm Corporation | High precision microfluidic devices and methods |
| CN101715483A (zh) | 2007-02-05 | 2010-05-26 | 微芯片生物工艺学股份有限公司 | 微流体和纳米流体装置、系统和应用 |
| WO2008134466A1 (en) | 2007-04-25 | 2008-11-06 | 3M Innovative Properties Company | Chemical component and processing device assembly |
| EP2152893B1 (en) | 2007-06-11 | 2014-02-26 | Wako Pure Chemical Industries, Ltd. | Method for nucleic acid amplification using a microchip PCR with integrated real-time CE detection |
| US20090053732A1 (en) | 2007-07-16 | 2009-02-26 | Ophir Vermesh | Microfluidic devices, methods and systems for detecting target molecules |
| JP2009041984A (ja) * | 2007-08-07 | 2009-02-26 | Arkray Inc | 分析装置、分析用具、および光学検知システム |
| WO2009029177A1 (en) | 2007-08-24 | 2009-03-05 | Dynamic Throughput Inc. | Integrated microfluidic optical device for sub-micro liter liquid sample microspectroscopy |
| US7736891B2 (en) | 2007-09-11 | 2010-06-15 | University Of Washington | Microfluidic assay system with dispersion monitoring |
| KR20090030084A (ko) | 2007-09-19 | 2009-03-24 | 삼성전자주식회사 | 미세유동장치 |
| WO2009038203A1 (ja) | 2007-09-21 | 2009-03-26 | Nec Corporation | 温度制御方法及びシステム |
| US20090087884A1 (en) | 2007-09-27 | 2009-04-02 | Timothy Beerling | Microfluidic nucleic acid amplification and separation |
| WO2009068583A2 (en) * | 2007-11-26 | 2009-06-04 | Atonomics A/S | Separation and detection device with means for optimization of the capillary drag force |
| EP2229578A4 (en) | 2008-01-07 | 2011-02-23 | Dynamic Throughput Inc | DISCOVERING TOOL WITH MICROFLUIDIC AND INTEGRATED BIOMARKER OPTICAL DETECTION NETWORK DEVICE, AND METHODS OF USE |
| WO2009108260A2 (en) | 2008-01-22 | 2009-09-03 | Microchip Biotechnologies, Inc. | Universal sample preparation system and use in an integrated analysis system |
| JP2009178078A (ja) * | 2008-01-30 | 2009-08-13 | Hitachi Engineering & Services Co Ltd | 微生物検査チップ及び微生物検査装置 |
| AU2009217355A1 (en) | 2008-02-21 | 2009-08-27 | Avantra Biosciences Corporation | Assays based on liquid flow over arrays |
| KR100952056B1 (ko) | 2008-02-29 | 2010-04-07 | 아주대학교산학협력단 | 기계적 자극에 대한 줄기세포 분화의 최적의 조건을 검출할수 있는 셀-칩 및 이의 자동 제어 시스템 |
| US8277759B2 (en) | 2008-03-04 | 2012-10-02 | The University Of Utah Research Foundation | Microfluidic flow cell |
| EP2280905B1 (en) | 2008-04-11 | 2016-07-06 | Fluidigm Corporation | Multilevel microfluidic systems and methods |
| WO2009136477A1 (ja) | 2008-05-07 | 2009-11-12 | パナソニック株式会社 | バイオセンサ |
| US20090325171A1 (en) | 2008-05-13 | 2009-12-31 | Thomas Hirt | Vesicles for use in biosensors |
| WO2010017210A1 (en) | 2008-08-07 | 2010-02-11 | Fluidigm Corporation | Microfluidic mixing and reaction systems for high efficiency screening |
| US7947492B2 (en) * | 2008-08-20 | 2011-05-24 | Northeastern Ohio Universities College Of Medicine | Device improving the detection of a ligand |
| WO2010027812A2 (en) | 2008-08-25 | 2010-03-11 | University Of Washington | Microfluidic systems incorporating flow-through membranes |
| US9156010B2 (en) | 2008-09-23 | 2015-10-13 | Bio-Rad Laboratories, Inc. | Droplet-based assay system |
| US9422409B2 (en) | 2008-10-10 | 2016-08-23 | Massachusetts Institute Of Technology | Method of hydrolytically stable bonding of elastomers to substrates |
| WO2010057078A2 (en) | 2008-11-14 | 2010-05-20 | The Brigham And Women's Hospital, Inc. | Method and system for generating spatially and temporally controllable concentration gradients |
| GB0821636D0 (en) | 2008-11-26 | 2008-12-31 | Ucl Business Plc | Device |
| CN105964316A (zh) | 2008-12-08 | 2016-09-28 | 富鲁达公司 | 可编程微流体数字阵列 |
| WO2010071045A1 (ja) | 2008-12-19 | 2010-06-24 | 公立大学法人大阪府立大学 | イムノアッセイ用キャピラリー及びそれを用いたキャピラリーイムノアッセイ法 |
| US8100293B2 (en) | 2009-01-23 | 2012-01-24 | Formulatrix, Inc. | Microfluidic dispensing assembly |
| US8017409B2 (en) | 2009-05-29 | 2011-09-13 | Ecolab Usa Inc. | Microflow analytical system |
| CN102459565A (zh) | 2009-06-02 | 2012-05-16 | 尹特根埃克斯有限公司 | 具有隔膜阀的流控设备 |
| US20120224053A1 (en) | 2009-06-17 | 2012-09-06 | Board Of Regents, The University Of Texas System | Method and apparatus for quantitative microimaging |
| US20120164036A1 (en) | 2009-07-21 | 2012-06-28 | Seth Stern | Microfluidic devices and uses thereof |
| SG169918A1 (en) | 2009-10-02 | 2011-04-29 | Fluidigm Corp | Microfluidic devices with removable cover and methods of fabrication and application |
| KR101472581B1 (ko) | 2009-10-21 | 2014-12-16 | 비오까르띠 엔브이 | 평행한 공압 계면판을 갖춘 마이크로 유체 카트리지 |
| WO2011053845A2 (en) | 2009-10-30 | 2011-05-05 | Illumina, Inc. | Microvessels, microparticles, and methods of manufacturing and using the same |
| CN103649759B (zh) | 2011-03-22 | 2016-08-31 | 西维克公司 | 微流体装置以及制造方法和用途 |
-
2010
- 2010-11-23 US US13/511,593 patent/US9229001B2/en active Active
- 2010-11-23 JP JP2012540157A patent/JP5701894B2/ja active Active
- 2010-11-23 CN CN201080062107.9A patent/CN102713621B/zh active Active
- 2010-11-23 EP EP10832371.8A patent/EP2504701B1/en active Active
- 2010-11-23 CA CA2781556A patent/CA2781556C/en active Active
- 2010-11-23 WO PCT/US2010/057860 patent/WO2011063408A1/en not_active Ceased
- 2010-11-23 ES ES10832371.8T patent/ES2649559T3/es active Active
-
2015
- 2015-02-18 JP JP2015029544A patent/JP5996014B2/ja active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CA2781556A1 (en) | 2011-05-26 |
| ES2649559T3 (es) | 2018-01-12 |
| CA2781556C (en) | 2015-05-19 |
| JP2015129762A (ja) | 2015-07-16 |
| CN102713621B (zh) | 2016-10-19 |
| CN102713621A (zh) | 2012-10-03 |
| EP2504701A4 (en) | 2013-08-21 |
| US9229001B2 (en) | 2016-01-05 |
| JP2013511731A (ja) | 2013-04-04 |
| EP2504701B1 (en) | 2017-09-13 |
| EP2504701A1 (en) | 2012-10-03 |
| US20130011859A1 (en) | 2013-01-10 |
| WO2011063408A1 (en) | 2011-05-26 |
| JP5996014B2 (ja) | 2016-10-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5701894B2 (ja) | アッセイを行う方法及び装置 | |
| WO2012071069A1 (en) | Method and apparatus for performing assays | |
| CN108535239B (zh) | 基于微液滴的微流控芯片和检测系统 | |
| JP2013511731A5 (enExample) | ||
| US7122153B2 (en) | Self-contained microfluidic biochip and apparatus | |
| JP5504690B2 (ja) | 分析チップ | |
| KR20220033484A (ko) | 자동화된 단일 세포 처리 및 분석을 위한 시스템 및 방법 | |
| He et al. | Composable microfluidic plates (cPlate): A simple and scalable fluid manipulation system for multiplexed enzyme-linked immunosorbent assay (ELISA) | |
| US11857957B2 (en) | Fluidic devices with reaction wells and uses thereof | |
| JP6871905B2 (ja) | アッセイを実行するための流体システム | |
| WO2005070533A1 (en) | System for characterising a fluid, microfluidic device for characterising or analysing concentrations components, a method of characterising or analysing such concentrations and a measurement | |
| CN111247433B (zh) | 分配设备、使用分配设备的分配装置及方法、以及检查装置及方法 | |
| WO2017213074A1 (ja) | 流体デバイス、システムおよび試料物質の検出方法 | |
| WO2007058077A1 (ja) | 遺伝子検査方法、遺伝子検査用マイクロリアクタ、および遺伝子検査システム | |
| Biswas et al. | Simple manual roller pump-driven valve-free microfluidic solution exchange system for urgent bioassay | |
| KR20170068356A (ko) | 반응액 저장 장치 및 이를 구비한 바이오 반응 장치 | |
| CN115003786A (zh) | 流动池、流动池进出液装置及样本分析系统 | |
| JP2006125990A (ja) | 生体物質検査デバイスおよびマイクロリアクタ | |
| JP2010063395A (ja) | 生体試料反応用チップ及び生体試料反応方法 | |
| JP2016148593A (ja) | 液体試料の検査方法 | |
| WO2009155935A1 (en) | Cartridge and system for liquid handling automation | |
| EP3325158A1 (en) | Reconfigurable microfluidic systems: scalable, multiplexed immunoassays | |
| JP2016031347A (ja) | 液体試料の検査方法 | |
| Li | Development of a microfluidic fluorescence immunosensor for Point-of-Care diagnostics | |
| JP2016148592A (ja) | 検査デバイスの製造方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20120731 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20120731 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20130306 |
|
| A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20130723 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20130806 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20131025 |
|
| A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20131101 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20131127 |
|
| A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20131204 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20131227 |
|
| A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20140110 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20140205 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20140819 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20141110 |
|
| A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20141117 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20141215 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20150120 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20150218 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 5701894 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |