CA2781556C - Method and apparatus for performing assays - Google Patents

Method and apparatus for performing assays Download PDF

Info

Publication number
CA2781556C
CA2781556C CA2781556A CA2781556A CA2781556C CA 2781556 C CA2781556 C CA 2781556C CA 2781556 A CA2781556 A CA 2781556A CA 2781556 A CA2781556 A CA 2781556A CA 2781556 C CA2781556 C CA 2781556C
Authority
CA
Canada
Prior art keywords
reaction vessel
microfluidic
assay
hollow element
valves
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2781556A
Other languages
English (en)
French (fr)
Other versions
CA2781556A1 (en
Inventor
Martin Andrew Putnam
Alan D. Kersey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CyVek Inc
Original Assignee
CyVek Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CyVek Inc filed Critical CyVek Inc
Publication of CA2781556A1 publication Critical patent/CA2781556A1/en
Application granted granted Critical
Publication of CA2781556C publication Critical patent/CA2781556C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1095—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers
    • G01N35/1097—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers characterised by the valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/52—Containers specially adapted for storing or dispensing a reagent
    • B01L3/527—Containers specially adapted for storing or dispensing a reagent for a plurality of reagents

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Dispersion Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA2781556A 2009-11-23 2010-11-23 Method and apparatus for performing assays Active CA2781556C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US26357209P 2009-11-23 2009-11-23
US61/263,572 2009-11-23
PCT/US2010/057860 WO2011063408A1 (en) 2009-11-23 2010-11-23 Method and apparatus for performing assays

Publications (2)

Publication Number Publication Date
CA2781556A1 CA2781556A1 (en) 2011-05-26
CA2781556C true CA2781556C (en) 2015-05-19

Family

ID=44060083

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2781556A Active CA2781556C (en) 2009-11-23 2010-11-23 Method and apparatus for performing assays

Country Status (7)

Country Link
US (1) US9229001B2 (enExample)
EP (1) EP2504701B1 (enExample)
JP (2) JP5701894B2 (enExample)
CN (1) CN102713621B (enExample)
CA (1) CA2781556C (enExample)
ES (1) ES2649559T3 (enExample)
WO (1) WO2011063408A1 (enExample)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110143378A1 (en) * 2009-11-12 2011-06-16 CyVek LLC. Microfluidic method and apparatus for high performance biological assays
WO2013142847A1 (en) * 2012-03-22 2013-09-26 Cyvek, Inc Pdms membrane-confined nucleic acid and antibody/antigen-functionalized microlength tube capture elements, and systems employing them
US9651568B2 (en) 2009-11-23 2017-05-16 Cyvek, Inc. Methods and systems for epi-fluorescent monitoring and scanning for microfluidic assays
WO2013134741A2 (en) * 2012-03-08 2013-09-12 Cyvek, Inc. Methods and systems for manufacture of microarray assay systems, conducting microfluidic assays, and monitoring and scanning to obtain microfluidic assay results
US9855735B2 (en) 2009-11-23 2018-01-02 Cyvek, Inc. Portable microfluidic assay devices and methods of manufacture and use
US10065403B2 (en) 2009-11-23 2018-09-04 Cyvek, Inc. Microfluidic assay assemblies and methods of manufacture
US9500645B2 (en) 2009-11-23 2016-11-22 Cyvek, Inc. Micro-tube particles for microfluidic assays and methods of manufacture
US9759718B2 (en) 2009-11-23 2017-09-12 Cyvek, Inc. PDMS membrane-confined nucleic acid and antibody/antigen-functionalized microlength tube capture elements, and systems employing them, and methods of their use
US9700889B2 (en) 2009-11-23 2017-07-11 Cyvek, Inc. Methods and systems for manufacture of microarray assay systems, conducting microfluidic assays, and monitoring and scanning to obtain microfluidic assay results
CN103649759B (zh) 2011-03-22 2016-08-31 西维克公司 微流体装置以及制造方法和用途
DE102012205171B3 (de) * 2012-03-29 2013-09-12 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Integriertes Einweg-Chipkartuschensystem für mobile Multiparameteranalysen chemischer und/oder biologischer Substanzen
US9458488B2 (en) * 2013-03-15 2016-10-04 Nanomix, Inc. Point of care sensor systems
WO2014150853A1 (en) * 2013-03-15 2014-09-25 Inanovate, Inc. Analyte measurement using longitudinal assay
US20170113221A1 (en) * 2014-06-11 2017-04-27 Micronics, Inc. Microfluidic cartridges and apparatus with integrated assay controls for analysis of nucleic acids
CN104568537A (zh) * 2014-11-05 2015-04-29 华文蔚 一种处理生物微流体样本的方法
EP3288681A1 (en) * 2015-04-30 2018-03-07 Orphidia Limited Microfluidic valves and devices
US10228367B2 (en) 2015-12-01 2019-03-12 ProteinSimple Segmented multi-use automated assay cartridge
US9933445B1 (en) 2016-05-16 2018-04-03 Hound Labs, Inc. System and method for target substance identification
CA3035286A1 (en) * 2016-10-07 2018-04-12 Boehringer Ingelheim Vetmedica Gmbh Method and analysis system for testing a sample
US20200245898A1 (en) * 2019-01-31 2020-08-06 Hound Labs, Inc. Single-use Microfluidic Cartridge for Detection of Target Chemical Presence in Human Breath
BR112021017166A2 (pt) * 2019-03-01 2021-11-09 Vidya Holdings Ltd Cartucho de ensaio para detectar um componente alvo em um fluido, e, método para fabricar um arranjo de cartuchos de ensaio
US11977086B2 (en) 2019-03-21 2024-05-07 Hound Labs, Inc. Biomarker detection from breath samples
US11933731B1 (en) 2020-05-13 2024-03-19 Hound Labs, Inc. Systems and methods using Surface-Enhanced Raman Spectroscopy for detecting tetrahydrocannabinol
US12392769B1 (en) 2021-01-12 2025-08-19 Hound Labs, Inc. Ambient contamination in breath analyte detection and measurement
CN114345430B (zh) * 2022-01-14 2022-10-28 北京工商大学 一种通过纸基微流控芯片同时检测多种抗生素残留的便携式装置
US20250128256A1 (en) * 2022-01-25 2025-04-24 President And Fellows Of Harvard College Microfluidic devices with autonomous directional valves

Family Cites Families (269)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5622871A (en) 1987-04-27 1997-04-22 Unilever Patent Holdings B.V. Capillary immunoassay and device therefor comprising mobilizable particulate labelled reagents
SE343949B (enExample) 1966-06-02 1972-03-20 Pharmacia Ab
US3867517A (en) 1971-12-21 1975-02-18 Abbott Lab Direct radioimmunoassay for antigens and their antibodies
US3939350A (en) 1974-04-29 1976-02-17 Board Of Trustees Of The Leland Stanford Junior University Fluorescent immunoassay employing total reflection for activation
US3876376A (en) 1974-05-09 1975-04-08 American Cyanamid Co Linear determination of hemolytic complement activity in undiluted serum
US4222744A (en) 1978-09-27 1980-09-16 Becton Dickinson & Company Assay for ligands
US4254096A (en) 1979-10-04 1981-03-03 Bio-Rad Laboratories, Inc. Reagent combination for solid phase immunofluorescent assay
US4517288A (en) 1981-01-23 1985-05-14 American Hospital Supply Corp. Solid phase system for ligand assay
US4425438A (en) 1981-03-13 1984-01-10 Bauman David S Assay method and device
US4368047A (en) 1981-04-27 1983-01-11 University Of Utah Research Foundation Process for conducting fluorescence immunoassays without added labels and employing attenuated internal reflection
NZ201901A (en) 1981-09-25 1986-11-12 Commw Serum Lab Commission An apparatus suitable for performing automated heterogeneous immunoassays in a plurality of samples
DE3226407C2 (de) 1982-07-15 1985-05-15 Volker Dr. 6900 Heidelberg Daniel Mikro-Analyse-Kapillar-System
US4447546A (en) 1982-08-23 1984-05-08 Myron J. Block Fluorescent immunoassay employing optical fiber in capillary tube
JPH0627742B2 (ja) 1982-12-21 1994-04-13 コムテツク リサ−チ ユニツト リミテツド 検定方法及びそのための装置
EP0149168B1 (en) 1983-12-19 1991-04-24 Daiichi Pure Chemicals Co. Ltd. Immunoassay
US4581624A (en) 1984-03-01 1986-04-08 Allied Corporation Microminiature semiconductor valve
JPH06105261B2 (ja) * 1984-03-05 1994-12-21 株式会社東芝 濃度勾配測定装置
US5004923A (en) 1985-08-05 1991-04-02 Biotrack, Inc. Capillary flow device
US5164598A (en) 1985-08-05 1992-11-17 Biotrack Capillary flow device
US4716121A (en) 1985-09-09 1987-12-29 Ord, Inc. Fluorescent assays, including immunoassays, with feature of flowing sample
US4844869A (en) 1985-09-09 1989-07-04 Ord, Inc. Immunoassay apparatus
US5500350A (en) 1985-10-30 1996-03-19 Celltech Limited Binding assay device
US4820490A (en) 1986-09-11 1989-04-11 Miles Inc. Device and method for chemical analysis of fluids with a reagent coated light source
US4717545A (en) 1986-09-11 1988-01-05 Miles Inc. Device and method for chemical analysis of fluids with a reagent coated light source
US4797259A (en) 1986-12-15 1989-01-10 Pall Corporation Well-type diagnostic plate device
US4923819A (en) 1987-03-27 1990-05-08 Chimerix Corporation Time-resolved fluorescence immunoassay
US4857453A (en) 1987-04-07 1989-08-15 Syntex (U.S.A.) Inc. Immunoassay device
DE3878820T2 (de) 1987-04-29 1993-07-22 Celltech Ltd Vorrichtung fuer bindungstest.
US5009998A (en) 1987-06-26 1991-04-23 E. I. Du Pont De Nemours And Company Method for performing heterogeneous immunoassay
US5041181A (en) 1987-10-06 1991-08-20 Integrated Fluidics Company Method of bonding plastics
US4908116A (en) 1989-06-01 1990-03-13 The Board Of Trustees At The Leland Stanford Junior University Capillary electrophoretic device employing structure permitting electrical contact through ionic movement
US5302349A (en) 1989-06-13 1994-04-12 Diatron Corporation Transient-state luminescence assay apparatus
US6008057A (en) 1989-08-25 1999-12-28 Roche Diagnostics Corporation Immunoassay system
US5183740A (en) 1990-02-23 1993-02-02 The United States Of America As Represented By The Secretary Of The Navy Flow immunosensor method and apparatus
SE470347B (sv) 1990-05-10 1994-01-31 Pharmacia Lkb Biotech Mikrostruktur för vätskeflödessystem och förfarande för tillverkning av ett sådant system
JPH06501099A (ja) 1990-09-11 1994-01-27 ヴォアヒーズ・テクノロジース・インコーポレーテッド 被覆された毛管
JP2523227Y2 (ja) 1991-07-30 1997-01-22 株式会社堀場製作所 異物検査装置
US5296375A (en) 1992-05-01 1994-03-22 Trustees Of The University Of Pennsylvania Mesoscale sperm handling devices
US5637469A (en) 1992-05-01 1997-06-10 Trustees Of The University Of Pennsylvania Methods and apparatus for the detection of an analyte utilizing mesoscale flow systems
US5304487A (en) 1992-05-01 1994-04-19 Trustees Of The University Of Pennsylvania Fluid handling in mesoscale analytical devices
US5885527A (en) * 1992-05-21 1999-03-23 Biosite Diagnostics, Inc. Diagnostic devices and apparatus for the controlled movement of reagents without membrances
JPH06108242A (ja) 1992-09-25 1994-04-19 Minolta Camera Co Ltd 薄膜電極および薄膜製造装置
US5508200A (en) 1992-10-19 1996-04-16 Tiffany; Thomas Method and apparatus for conducting multiple chemical assays
US5534328A (en) 1993-12-02 1996-07-09 E. I. Du Pont De Nemours And Company Integrated chemical processing apparatus and processes for the preparation thereof
US5493802A (en) 1993-05-26 1996-02-27 Simson; Anton K. Scroll displaying device
US5837546A (en) 1993-08-24 1998-11-17 Metrika, Inc. Electronic assay device and method
ATE224053T1 (de) 1993-11-12 2002-09-15 Inverness Medical Switzerland Vorrichtung zum ablesen von teststreifen
US5976896A (en) 1994-06-06 1999-11-02 Idexx Laboratories, Inc. Immunoassays in capillary tubes
US5624850A (en) 1994-06-06 1997-04-29 Idetek, Inc. Immunoassays in capillaries
US5593290A (en) 1994-12-22 1997-01-14 Eastman Kodak Company Micro dispensing positive displacement pump
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US6068751A (en) 1995-12-18 2000-05-30 Neukermans; Armand P. Microfluidic valve and integrated microfluidic system
US6541213B1 (en) 1996-03-29 2003-04-01 University Of Washington Microscale diffusion immunoassay
US5788814A (en) 1996-04-09 1998-08-04 David Sarnoff Research Center Chucks and methods for positioning multiple objects on a substrate
US5942443A (en) 1996-06-28 1999-08-24 Caliper Technologies Corporation High throughput screening assay systems in microscale fluidic devices
US5885470A (en) 1997-04-14 1999-03-23 Caliper Technologies Corporation Controlled fluid transport in microfabricated polymeric substrates
JPH09288089A (ja) 1996-04-23 1997-11-04 Hitachi Ltd 毛細管電気泳動装置
CA2253710A1 (en) 1996-04-25 1997-10-30 Spectrametrix Inc. Analyte assay using particulate labels
US7341727B1 (en) 1996-05-03 2008-03-11 Emergent Product Development Gaithersburg Inc. M. catarrhalis outer membrane protein-106 polypeptide, methods of eliciting an immune response comprising same
NZ333346A (en) 1996-06-28 2000-03-27 Caliper Techn Corp High-throughput screening assay systems in microscale fluidic devices
US20030054376A1 (en) 1997-07-07 2003-03-20 Mullis Kary Banks Dual bead assays using cleavable spacers and/or ligation to improve specificity and sensitivity including related methods and apparatus
DE19635646C1 (de) 1996-09-03 1997-12-04 Bruker Franzen Analytik Gmbh Korrektur der Massenbestimmung mit MALDI-Flugzeitmassenspektrometern
EP0990142A4 (en) 1996-12-31 2000-09-27 Genometrix Genomics Inc MULTIPLEXED MOLECULAR ANALYSIS METHOD AND DEVICE
US6507989B1 (en) 1997-03-13 2003-01-21 President And Fellows Of Harvard College Self-assembly of mesoscale objects
US6391622B1 (en) 1997-04-04 2002-05-21 Caliper Technologies Corp. Closed-loop biochemical analyzers
KR100351531B1 (ko) 1997-04-25 2002-09-11 캘리퍼 테크놀로지스 코포레이션 기하형상이 개선된 채널을 채용하는 미소 유체 장치
US6020209A (en) 1997-04-28 2000-02-01 The United States Of America As Represented By The Secretary Of The Navy Microcapillary-based flow-through immunosensor and displacement immunoassay using the same
US5882465A (en) 1997-06-18 1999-03-16 Caliper Technologies Corp. Method of manufacturing microfluidic devices
US5932799A (en) 1997-07-21 1999-08-03 Ysi Incorporated Microfluidic analyzer module
US6073482A (en) 1997-07-21 2000-06-13 Ysi Incorporated Fluid flow module
US6293012B1 (en) 1997-07-21 2001-09-25 Ysi Incorporated Method of making a fluid flow module
US6082185A (en) 1997-07-25 2000-07-04 Research International, Inc. Disposable fluidic circuit cards
US5876675A (en) 1997-08-05 1999-03-02 Caliper Technologies Corp. Microfluidic devices and systems
JP3938982B2 (ja) 1997-08-29 2007-06-27 オリンパス株式会社 Ｄｎａキャピラリィ
US6103537A (en) 1997-10-02 2000-08-15 Aclara Biosciences, Inc. Capillary assays involving separation of free and bound species
US5842787A (en) 1997-10-09 1998-12-01 Caliper Technologies Corporation Microfluidic systems incorporating varied channel dimensions
US5965237A (en) 1997-10-20 1999-10-12 Novartis Ag Microstructure device
US6143152A (en) 1997-11-07 2000-11-07 The Regents Of The University Of California Microfabricated capillary array electrophoresis device and method
US6167910B1 (en) 1998-01-20 2001-01-02 Caliper Technologies Corp. Multi-layer microfluidic devices
US6251343B1 (en) 1998-02-24 2001-06-26 Caliper Technologies Corp. Microfluidic devices and systems incorporating cover layers
US6756019B1 (en) 1998-02-24 2004-06-29 Caliper Technologies Corp. Microfluidic devices and systems incorporating cover layers
US7497994B2 (en) 1998-02-24 2009-03-03 Khushroo Gandhi Microfluidic devices and systems incorporating cover layers
US6100541A (en) 1998-02-24 2000-08-08 Caliper Technologies Corporation Microfluidic devices and systems incorporating integrated optical elements
US6719868B1 (en) 1998-03-23 2004-04-13 President And Fellows Of Harvard College Methods for fabricating microfluidic structures
JPH11307604A (ja) 1998-04-17 1999-11-05 Toshiba Corp プロセスモニタ方法及びプロセス装置
US6787111B2 (en) 1998-07-02 2004-09-07 Amersham Biosciences (Sv) Corp. Apparatus and method for filling and cleaning channels and inlet ports in microchips used for biological analysis
US6576478B1 (en) 1998-07-14 2003-06-10 Zyomyx, Inc. Microdevices for high-throughput screening of biomolecules
US6908770B1 (en) 1998-07-16 2005-06-21 Board Of Regents, The University Of Texas System Fluid based analysis of multiple analytes by a sensor array
US20020049694A1 (en) 1998-07-27 2002-04-25 J. Wallace Parce Distributed database for analytical instruments
US7155344B1 (en) 1998-07-27 2006-12-26 Caliper Life Sciences, Inc. Distributed database for analytical instruments
US6086740A (en) 1998-10-29 2000-07-11 Caliper Technologies Corp. Multiplexed microfluidic devices and systems
US6497155B1 (en) 1999-02-09 2002-12-24 Pharmacopeia, Inc. Article comprising a particle retrieval device
US6303343B1 (en) 1999-04-06 2001-10-16 Caliper Technologies Corp. Inefficient fast PCR
US20050100943A1 (en) 2000-04-11 2005-05-12 Hideki Kambara Method of producing probe arrays for biological materials using fine particles
US6908737B2 (en) 1999-04-15 2005-06-21 Vitra Bioscience, Inc. Systems and methods of conducting multiplexed experiments
US20040200909A1 (en) 1999-05-28 2004-10-14 Cepheid Apparatus and method for cell disruption
AU5291600A (en) 1999-06-01 2000-12-18 Caliper Technologies Corporation Microscale assays and microfluidic devices for transporter, gradient induced, and binding activities
US6520753B1 (en) 1999-06-04 2003-02-18 California Institute Of Technology Planar micropump
US6929030B2 (en) 1999-06-28 2005-08-16 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US7144616B1 (en) 1999-06-28 2006-12-05 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US6899137B2 (en) 1999-06-28 2005-05-31 California Institute Of Technology Microfabricated elastomeric valve and pump systems
EP1065378B1 (en) 1999-06-28 2002-04-03 California Institute of Technology Microfabricated elastomeric valve and pump systems
US6533914B1 (en) 1999-07-08 2003-03-18 Shaorong Liu Microfabricated injector and capillary array assembly for high-resolution and high throughput separation
AU6366200A (en) * 1999-07-27 2001-02-13 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
US6762059B2 (en) * 1999-08-13 2004-07-13 U.S. Genomics, Inc. Methods and apparatuses for characterization of single polymers
AU6932300A (en) * 1999-08-25 2001-03-19 Caliper Technologies Corporation Dilutions in high throughput systems with a single vacuum source
US6383748B1 (en) 1999-09-14 2002-05-07 Pamgene B.V. Analytical test device with substrate having oriented through going channels and improved methods and apparatus for using same
US6361958B1 (en) 1999-11-12 2002-03-26 Motorola, Inc. Biochannel assay for hybridization with biomaterial
US6875619B2 (en) 1999-11-12 2005-04-05 Motorola, Inc. Microfluidic devices comprising biochannels
JP3441058B2 (ja) 1999-12-03 2003-08-25 理化学研究所 キャピラリーゲル電気泳動用マイクロチップおよびその製造方法
WO2001045843A2 (en) 1999-12-22 2001-06-28 Gene Logic, Inc. Flow-thru chip cartridge, chip holder, system and method thereof
CA2401782A1 (en) 2000-01-31 2001-08-02 John T. Mcdevitt Portable sensor array system
AU776266C (en) 2000-01-31 2006-01-05 Diagnoswiss S.A. Method for fabricating micro-structures with various surface properties in multilayer body by plasma etching
US7867763B2 (en) 2004-01-25 2011-01-11 Fluidigm Corporation Integrated chip carriers with thermocycler interfaces and methods of using the same
US7351376B1 (en) 2000-06-05 2008-04-01 California Institute Of Technology Integrated active flux microfluidic devices and methods
CA2415055A1 (en) 2000-08-03 2002-02-14 Caliper Technologies Corporation Methods and devices for high throughput fluid delivery
US7189358B2 (en) 2000-08-08 2007-03-13 California Institute Of Technology Integrated micropump analysis chip and method of making the same
US7998746B2 (en) 2000-08-24 2011-08-16 Robert Otillar Systems and methods for localizing and analyzing samples on a bio-sensor chip
AU2001290879A1 (en) 2000-09-15 2002-03-26 California Institute Of Technology Microfabricated crossflow devices and methods
US6994826B1 (en) 2000-09-26 2006-02-07 Sandia National Laboratories Method and apparatus for controlling cross contamination of microfluid channels
JP2002122596A (ja) * 2000-10-16 2002-04-26 Mitsubishi Rayon Co Ltd 生体関連物質検出用マイクロアレイ
US20050221385A1 (en) 2000-11-07 2005-10-06 Caliper Life Sciences, Inc. Pressure based mobility shift assays
AU2002230524A1 (en) 2000-11-16 2002-05-27 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
GB0028647D0 (en) 2000-11-24 2001-01-10 Nextgen Sciences Ltd Apparatus for chemical assays
JP4248238B2 (ja) 2001-01-08 2009-04-02 プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ マイクロ流体システム用の弁およびポンプならびにマイクロ流体システムを作製するための方法
US20060207877A1 (en) 2001-01-30 2006-09-21 Walter Schmidt Microfluidic device with various surface properties fabricated in multilayer body by plasma etching
JP2002286717A (ja) * 2001-03-23 2002-10-03 Central Res Inst Of Electric Power Ind 無蛍光担体およびこれを固相に用いた測定用セル、ならびに無蛍光担体を利用する濃度測定方法
WO2002081729A2 (en) 2001-04-06 2002-10-17 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
JP2002340900A (ja) * 2001-05-18 2002-11-27 Mitsubishi Rayon Co Ltd バイオセンサー、バイオセンサーアレイ及び検出方法
US7318912B2 (en) 2001-06-07 2008-01-15 Nanostream, Inc. Microfluidic systems and methods for combining discrete fluid volumes
US6729352B2 (en) 2001-06-07 2004-05-04 Nanostream, Inc. Microfluidic synthesis devices and methods
GB0116384D0 (en) 2001-07-04 2001-08-29 Diagnoswiss Sa Microfluidic chemical assay apparatus and method
US6881579B2 (en) 2001-07-30 2005-04-19 Agilent Technologies, Inc. Sample processing apparatus and methods
WO2003031066A1 (en) 2001-10-11 2003-04-17 California Institute Of Technology Devices utilizing self-assembled gel and method of manufacture
US6750661B2 (en) 2001-11-13 2004-06-15 Caliper Life Sciences, Inc. Method and apparatus for controllably effecting samples using two signals
US7691333B2 (en) 2001-11-30 2010-04-06 Fluidigm Corporation Microfluidic device and methods of using same
EP1461606A4 (en) 2001-12-05 2005-06-29 Univ Washington MICROFLUIDIC DEVICE AND SURFACE DECORATION PROCESS FOR SOLID PHASE AFFINITY BINDING ASSAYS
US6532997B1 (en) 2001-12-28 2003-03-18 3M Innovative Properties Company Sample processing device with integral electrophoresis channels
US7622083B2 (en) 2002-01-28 2009-11-24 Biocal Technology, Inc. Multi-capillary electrophoresis cartridge interface mechanism
WO2003078045A2 (en) 2002-03-12 2003-09-25 Syngenta Participations Ag Microcapillary hybridization chamber
US7524462B2 (en) 2002-03-29 2009-04-28 Agilent Technologies, Inc. Capillary flow for a heterogenous assay in a micro-channel environment
US8211657B2 (en) 2002-04-29 2012-07-03 The Board Of Trustees Of The University Of Arkansas Capillary-column-based bioseparator/bioreactor with an optical/electrochemical detector for detection of microbial pathogens
US7125510B2 (en) 2002-05-15 2006-10-24 Zhili Huang Microstructure fabrication and microsystem integration
EP2093565B1 (en) 2002-05-22 2014-11-19 Platypus Technologies, Inc. Uses of devices and methods for cellular assays
AU2003240738A1 (en) 2002-06-03 2003-12-19 Pamgene Bv Method for high-throughput integrated chemical and biochemical reactions
AU2003277853A1 (en) 2002-06-24 2004-01-06 Fluidigm Corporation Recirculating fluidic network and methods for using the same
US20040101444A1 (en) 2002-07-15 2004-05-27 Xeotron Corporation Apparatus and method for fluid delivery to a hybridization station
US7164533B2 (en) 2003-01-22 2007-01-16 Cyvera Corporation Hybrid random bead/chip based microarray
AU2003270004A1 (en) 2002-08-28 2004-03-19 Mt Technologies, Inc. Microfluidic affinity system using polydimethylsiloxane and a surface modification process
EP1540590A1 (en) 2002-09-12 2005-06-15 Cyvera Corporation Assay stick comprising coded microbeads
US20100255603A9 (en) 2002-09-12 2010-10-07 Putnam Martin A Method and apparatus for aligning microbeads in order to interrogate the same
CA2499046A1 (en) 2002-09-12 2004-03-25 Cyvera Corporation Diffraction grating-based encoded micro-particles for multiplexed experiments
WO2004024328A1 (en) 2002-09-12 2004-03-25 Cyvera Corporation Method and apparatus for aligning elongated microbeads in order to interrogate the same
JP2006501056A (ja) 2002-09-25 2006-01-12 カリフォルニア インスティテュート オブ テクノロジー ミクロ流体大規模集積
US7186383B2 (en) 2002-09-27 2007-03-06 Ast Management Inc. Miniaturized fluid delivery and analysis system
TW590982B (en) 2002-09-27 2004-06-11 Agnitio Science & Technology I Micro-fluid driving device
EP1560642A4 (en) 2002-10-09 2006-05-03 Univ Illinois MICROFLUID SYSTEMS AND COMPONENTS
US7390457B2 (en) 2002-10-31 2008-06-24 Agilent Technologies, Inc. Integrated microfluidic array device
WO2004059299A1 (en) 2002-12-16 2004-07-15 Cytodiscovery, Inc. Microfluidic system with integrated permeable membrane
US20050266582A1 (en) 2002-12-16 2005-12-01 Modlin Douglas N Microfluidic system with integrated permeable membrane
CA2521999A1 (en) 2002-12-20 2004-09-02 Biotrove, Inc. Assay apparatus and method using microfluidic arrays
CN102620959B (zh) 2002-12-26 2015-12-16 梅索磅秤技术有限公司 检定盒及其使用方法
KR101216828B1 (ko) 2002-12-30 2013-01-04 더 리전트 오브 더 유니버시티 오브 캘리포니아 병원균 검출과 분석을 위한 방법과 기구
US7033476B2 (en) 2002-12-31 2006-04-25 Ut-Battelle, Llc Separation and counting of single molecules through nanofluidics, programmable electrophoresis, and nanoelectrode-gated tunneling and dielectric detection
US7122153B2 (en) 2003-01-08 2006-10-17 Ho Winston Z Self-contained microfluidic biochip and apparatus
US7476363B2 (en) 2003-04-03 2009-01-13 Fluidigm Corporation Microfluidic devices and methods of using same
TW579430B (en) 2003-05-02 2004-03-11 Dr Chip Biotechnology Inc Automatic micro-fluid hybridization chip platform
US7695683B2 (en) 2003-05-20 2010-04-13 Fluidigm Corporation Method and system for microfluidic device and imaging thereof
US7238269B2 (en) 2003-07-01 2007-07-03 3M Innovative Properties Company Sample processing device with unvented channel
FR2857099B1 (fr) 2003-07-04 2005-10-07 Jean Marie Billiotte Procede et dispositif d'analyse chimique ou biologique par senseur a chambre monolithique en gerbe multi-micro-tubulaire et transducteur lateral de mesure integrale
US7341841B2 (en) * 2003-07-12 2008-03-11 Accelr8 Technology Corporation Rapid microbial detection and antimicrobial susceptibility testing
US7028536B2 (en) 2004-06-29 2006-04-18 Nanostream, Inc. Sealing interface for microfluidic device
US20050098750A1 (en) 2003-11-06 2005-05-12 Daniel Sobek Electrostatic sealing device and method of use thereof
JP4073023B2 (ja) 2003-11-07 2008-04-09 財団法人新産業創造研究機構 微小流路デバイスおよびその作製方法
US7943089B2 (en) 2003-12-19 2011-05-17 Kimberly-Clark Worldwide, Inc. Laminated assay devices
CA2834041C (en) 2003-12-31 2017-05-16 President And Fellows Of Harvard College Assay device and method
WO2005083390A1 (en) * 2004-02-20 2005-09-09 Research Foundation Of The State University Of New York Method and device for manipulating liquids in microfluidic systems
EP3270156A1 (en) 2004-04-07 2018-01-17 Abbott Laboratories Disposable chamber for analyzing biologic fluids
JP5502275B2 (ja) 2004-05-02 2014-05-28 フルイディグム コーポレイション 熱反応デバイスおよびその熱反応デバイスの使用方法
US7887750B2 (en) 2004-05-05 2011-02-15 Bayer Healthcare Llc Analytical systems, devices, and cartridges therefor
US7419639B2 (en) 2004-05-12 2008-09-02 The Board Of Trustees Of The Leland Stanford Junior University Multilayer microfluidic device
US7799553B2 (en) 2004-06-01 2010-09-21 The Regents Of The University Of California Microfabricated integrated DNA analysis system
EP1776581B1 (en) 2004-07-19 2015-05-06 ProteinSimple Method for protein detection
JP2006098169A (ja) * 2004-09-29 2006-04-13 Fujitsu Ltd 被検体定量デバイスおよび被検体定量方法
EP1794581A2 (en) 2004-09-15 2007-06-13 Microchip Biotechnologies, Inc. Microfluidic devices
US7832429B2 (en) 2004-10-13 2010-11-16 Rheonix, Inc. Microfluidic pump and valve structures and fabrication methods
CN101036056A (zh) 2004-10-29 2007-09-12 伊藤火腿株式会社 反应容器
JP2008522795A (ja) 2004-12-03 2008-07-03 カリフォルニア インスティチュート オブ テクノロジー 化学反応回路を有するマイクロ流体装置
US7682817B2 (en) 2004-12-23 2010-03-23 Kimberly-Clark Worldwide, Inc. Microfluidic assay devices
US20060228734A1 (en) * 2005-03-18 2006-10-12 Applera Corporation Fluid processing device with captured reagent beads
DE102005049365A1 (de) 2005-03-18 2006-09-21 BAM Bundesanstalt für Materialforschung und -prüfung Kalibriereinrichtung und Farbstoffkit sowie ihre Verwendung zur Charakterisierung von Lumineszenzmesssystemen
CA2602493C (en) * 2005-03-23 2015-03-17 Velocys, Inc. Surface features in microprocess technology
JP2008538609A (ja) 2005-04-20 2008-10-30 ベクトン・ディキンソン・アンド・カンパニー マルチプレックスマイクロ粒子システム
CA2505657A1 (en) 2005-04-28 2006-10-28 York University Method for mixing inside a capillary and device for achieving same
WO2006122311A2 (en) 2005-05-11 2006-11-16 The Trustees Of The University Of Pennsylvania Microfluidic chip
JPWO2006123578A1 (ja) 2005-05-19 2008-12-25 コニカミノルタエムジー株式会社 検体中の標的物質を分析するための検査チップおよびマイクロ総合分析システム
EP1884766A4 (en) * 2005-05-20 2013-10-16 Hitachi Chemical Co Ltd BIOCHEMICAL ANALYSIS PROCESS
US20060263818A1 (en) * 2005-05-23 2006-11-23 Axel Scherer High throughput multi-antigen microfluidic fluorescence immunoassays
EP2477029A1 (en) 2005-06-02 2012-07-18 Fluidigm Corporation Analysis using microfluidic partitioning devices
US20090142853A1 (en) 2005-08-11 2009-06-04 Eksigent Technologies, Llc Microfluidic system and methods
RO122612B1 (ro) 2005-08-29 2009-09-30 Institutul Naţional De Cercetare-Dezvoltare Pentru Microtehnologie Procedeu de realizare a unui biocip cu funcţia de amplificare a unor fragmente specifice de adn prin reacţia polimerazică în lan
US20070074972A1 (en) 2005-09-13 2007-04-05 Fluidigm Corporation Microfluidic assay devices and methods
JP3116709U (ja) * 2005-09-13 2005-12-15 有限会社メタボスクリーン 微小流路チップ
US20080311585A1 (en) 2005-11-02 2008-12-18 Affymetrix, Inc. System and method for multiplex liquid handling
US7763453B2 (en) 2005-11-30 2010-07-27 Micronics, Inc. Microfluidic mixing and analytic apparatus
JP4253323B2 (ja) * 2005-12-05 2009-04-08 株式会社東芝 マイクロ化学チップ
US8182767B2 (en) 2005-12-27 2012-05-22 Honeywell International Inc. Needle-septum interface for a fluidic analyzer
WO2007092713A2 (en) 2006-02-02 2007-08-16 Trustees Of The University Of Pennsylvania Microfluidic system and method for analysis of gene expression in cell-containing samples and detection of disease
EP1979079A4 (en) 2006-02-03 2012-11-28 Integenx Inc MICROFLUIDIC DEVICES
US8124015B2 (en) 2006-02-03 2012-02-28 Institute For Systems Biology Multiplexed, microfluidic molecular assay device and assay method
EP1987275A1 (en) 2006-02-13 2008-11-05 Koninklijke Philips Electronics N.V. Microfluidic device for molecular diagnostic applications
US7639026B2 (en) 2006-02-24 2009-12-29 Johnstech International Corporation Electronic device test set and contact used therein
CN101067642A (zh) * 2006-02-24 2007-11-07 丹尼斯·B·歇尔 电子器件测试装置及其所使用的触头
WO2007106580A2 (en) * 2006-03-15 2007-09-20 Micronics, Inc. Rapid magnetic flow assays
US7766033B2 (en) 2006-03-22 2010-08-03 The Regents Of The University Of California Multiplexed latching valves for microfluidic devices and processors
US8741230B2 (en) 2006-03-24 2014-06-03 Theranos, Inc. Systems and methods of sample processing and fluid control in a fluidic system
US8293524B2 (en) 2006-03-31 2012-10-23 Fluxion Biosciences Inc. Methods and apparatus for the manipulation of particle suspensions and testing thereof
WO2007125468A2 (en) 2006-05-01 2007-11-08 Koninklijke Philips Electronics N.V. Fluid sample transport device with reduced dead volume for processing, controlling and/or detecting a fluid sample
AU2007254191B2 (en) 2006-05-16 2013-01-10 Fluidigm Corporation PCR-free sample preparation and detection systems for high speed biologic analysis and identification
EP2041573B1 (en) 2006-06-23 2019-09-04 PerkinElmer Health Sciences, Inc. Methods and devices for microfluidic point-of-care immunoassays
CA2656686A1 (en) 2006-07-05 2008-01-10 Aida Engineering, Ltd. Micro passage chip and fluid transferring method
US7794665B2 (en) * 2006-07-17 2010-09-14 Industrial Technology Research Institute Fluidic device
US20080017512A1 (en) 2006-07-24 2008-01-24 Bordunov Andrei V Coatings for capillaries capable of capturing analytes
GR1006447B (el) 2006-09-15 2009-06-19 Εθνικο Κεντρο Ερευνας Φυσικων Επιστημων (Εκεφε) "Δημοκριτος" Μεθοδος συγκολλησης
WO2008147382A1 (en) 2006-09-27 2008-12-04 Micronics, Inc. Integrated microfluidic assay devices and methods
US20080131327A1 (en) 2006-09-28 2008-06-05 California Institute Of Technology System and method for interfacing with a microfluidic chip
EP2074341A4 (en) 2006-10-04 2013-04-10 Fluidigm Corp MICROFLUIDIC CHECK VALVES
WO2008043041A1 (en) 2006-10-04 2008-04-10 University Of Washington Method and device for rapid parallel microfluidic molecular affinity assays
EP2084104A1 (en) 2006-11-03 2009-08-05 McGill University Electrical microvalve and method of manufacturing thereof
US8425863B2 (en) 2006-12-19 2013-04-23 Koninklijke Philips Electronics N.V. Micro fluidic device
EP2125219B1 (en) 2007-01-19 2016-08-10 Fluidigm Corporation High precision microfluidic devices and methods
CN101715483A (zh) 2007-02-05 2010-05-26 微芯片生物工艺学股份有限公司 微流体和纳米流体装置、系统和应用
WO2008134466A1 (en) 2007-04-25 2008-11-06 3M Innovative Properties Company Chemical component and processing device assembly
EP2152893B1 (en) 2007-06-11 2014-02-26 Wako Pure Chemical Industries, Ltd. Method for nucleic acid amplification using a microchip PCR with integrated real-time CE detection
US20090053732A1 (en) 2007-07-16 2009-02-26 Ophir Vermesh Microfluidic devices, methods and systems for detecting target molecules
JP2009041984A (ja) * 2007-08-07 2009-02-26 Arkray Inc 分析装置、分析用具、および光学検知システム
WO2009029177A1 (en) 2007-08-24 2009-03-05 Dynamic Throughput Inc. Integrated microfluidic optical device for sub-micro liter liquid sample microspectroscopy
US7736891B2 (en) 2007-09-11 2010-06-15 University Of Washington Microfluidic assay system with dispersion monitoring
KR20090030084A (ko) 2007-09-19 2009-03-24 삼성전자주식회사 미세유동장치
WO2009038203A1 (ja) 2007-09-21 2009-03-26 Nec Corporation 温度制御方法及びシステム
US20090087884A1 (en) 2007-09-27 2009-04-02 Timothy Beerling Microfluidic nucleic acid amplification and separation
WO2009068583A2 (en) * 2007-11-26 2009-06-04 Atonomics A/S Separation and detection device with means for optimization of the capillary drag force
EP2229578A4 (en) 2008-01-07 2011-02-23 Dynamic Throughput Inc DISCOVERING TOOL WITH MICROFLUIDIC AND INTEGRATED BIOMARKER OPTICAL DETECTION NETWORK DEVICE, AND METHODS OF USE
WO2009108260A2 (en) 2008-01-22 2009-09-03 Microchip Biotechnologies, Inc. Universal sample preparation system and use in an integrated analysis system
JP2009178078A (ja) * 2008-01-30 2009-08-13 Hitachi Engineering & Services Co Ltd 微生物検査チップ及び微生物検査装置
AU2009217355A1 (en) 2008-02-21 2009-08-27 Avantra Biosciences Corporation Assays based on liquid flow over arrays
KR100952056B1 (ko) 2008-02-29 2010-04-07 아주대학교산학협력단 기계적 자극에 대한 줄기세포 분화의 최적의 조건을 검출할수 있는 셀-칩 및 이의 자동 제어 시스템
US8277759B2 (en) 2008-03-04 2012-10-02 The University Of Utah Research Foundation Microfluidic flow cell
EP2280905B1 (en) 2008-04-11 2016-07-06 Fluidigm Corporation Multilevel microfluidic systems and methods
WO2009136477A1 (ja) 2008-05-07 2009-11-12 パナソニック株式会社 バイオセンサ
US20090325171A1 (en) 2008-05-13 2009-12-31 Thomas Hirt Vesicles for use in biosensors
WO2010017210A1 (en) 2008-08-07 2010-02-11 Fluidigm Corporation Microfluidic mixing and reaction systems for high efficiency screening
US7947492B2 (en) * 2008-08-20 2011-05-24 Northeastern Ohio Universities College Of Medicine Device improving the detection of a ligand
WO2010027812A2 (en) 2008-08-25 2010-03-11 University Of Washington Microfluidic systems incorporating flow-through membranes
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9422409B2 (en) 2008-10-10 2016-08-23 Massachusetts Institute Of Technology Method of hydrolytically stable bonding of elastomers to substrates
WO2010057078A2 (en) 2008-11-14 2010-05-20 The Brigham And Women's Hospital, Inc. Method and system for generating spatially and temporally controllable concentration gradients
GB0821636D0 (en) 2008-11-26 2008-12-31 Ucl Business Plc Device
CN105964316A (zh) 2008-12-08 2016-09-28 富鲁达公司 可编程微流体数字阵列
WO2010071045A1 (ja) 2008-12-19 2010-06-24 公立大学法人大阪府立大学 イムノアッセイ用キャピラリー及びそれを用いたキャピラリーイムノアッセイ法
US8100293B2 (en) 2009-01-23 2012-01-24 Formulatrix, Inc. Microfluidic dispensing assembly
US8017409B2 (en) 2009-05-29 2011-09-13 Ecolab Usa Inc. Microflow analytical system
CN102459565A (zh) 2009-06-02 2012-05-16 尹特根埃克斯有限公司 具有隔膜阀的流控设备
US20120224053A1 (en) 2009-06-17 2012-09-06 Board Of Regents, The University Of Texas System Method and apparatus for quantitative microimaging
US20120164036A1 (en) 2009-07-21 2012-06-28 Seth Stern Microfluidic devices and uses thereof
SG169918A1 (en) 2009-10-02 2011-04-29 Fluidigm Corp Microfluidic devices with removable cover and methods of fabrication and application
KR101472581B1 (ko) 2009-10-21 2014-12-16 비오까르띠 엔브이 평행한 공압 계면판을 갖춘 마이크로 유체 카트리지
WO2011053845A2 (en) 2009-10-30 2011-05-05 Illumina, Inc. Microvessels, microparticles, and methods of manufacturing and using the same
CN103649759B (zh) 2011-03-22 2016-08-31 西维克公司 微流体装置以及制造方法和用途

Also Published As

Publication number Publication date
JP5701894B2 (ja) 2015-04-15
CA2781556A1 (en) 2011-05-26
ES2649559T3 (es) 2018-01-12
JP2015129762A (ja) 2015-07-16
CN102713621B (zh) 2016-10-19
CN102713621A (zh) 2012-10-03
EP2504701A4 (en) 2013-08-21
US9229001B2 (en) 2016-01-05
JP2013511731A (ja) 2013-04-04
EP2504701B1 (en) 2017-09-13
EP2504701A1 (en) 2012-10-03
US20130011859A1 (en) 2013-01-10
WO2011063408A1 (en) 2011-05-26
JP5996014B2 (ja) 2016-10-25

Similar Documents

Publication Publication Date Title
CA2781556C (en) Method and apparatus for performing assays
WO2012071069A1 (en) Method and apparatus for performing assays
US11938710B2 (en) Microfluidic assay assemblies and methods of manufacture
CN112980667B (zh) 用于分离和分析细胞的系统和方法
JP2013511731A5 (enExample)
US8133456B2 (en) Microreactor and method of liquid feeding making use of the same
US9500645B2 (en) Micro-tube particles for microfluidic assays and methods of manufacture
US10076752B2 (en) Methods and systems for manufacture of microarray assay systems, conducting microfluidic assays, and monitoring and scanning to obtain microfluidic assay results
CN112827517B (zh) 一种微流控芯片的使用方法及其装置
US20100068098A1 (en) Microfluidic devices and methods for multiple analyte detection
EP2822688A2 (en) Microfluidic assay assemblies and methods of manufacture
WO2018200896A1 (en) Fluidic devices with reaction wells and uses thereof
CN110665557B (zh) 多孔基底和嵌入生物测定中的微多孔基底
CN119335191A (zh) 一种单分子免疫检测方法及单分子芯片
CN100504389C (zh) 应用微流体传送及分析系统的方法
WO2008063406A2 (en) A platform for binding assays with dual multiplexing capability
CN104995515A (zh) 新的床旁检测系统和方法
KR20170068356A (ko) 반응액 저장 장치 및 이를 구비한 바이오 반응 장치
US20080021364A1 (en) Fluidic device
JP2016148593A (ja) 液体試料の検査方法
RU200301U1 (ru) Микрофлюидный чип для проведения многопараметрического иммуноанализа
EP3325158A1 (en) Reconfigurable microfluidic systems: scalable, multiplexed immunoassays
JP2016148592A (ja) 検査デバイスの製造方法
JP2016109537A (ja) 送液方法
JP2016148583A (ja) 検査デバイスの製造方法

Legal Events

Date Code Title Description
EEER Examination request