JP4073023B2 - 微小流路デバイスおよびその作製方法 - Google Patents
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- JP4073023B2 JP4073023B2 JP2003378634A JP2003378634A JP4073023B2 JP 4073023 B2 JP4073023 B2 JP 4073023B2 JP 2003378634 A JP2003378634 A JP 2003378634A JP 2003378634 A JP2003378634 A JP 2003378634A JP 4073023 B2 JP4073023 B2 JP 4073023B2
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Description
(1)ガラスモールドの作製(図1の左半部に示す)
幅300μmの刃を有するダイシングソーを用いて、モールド用基板としての正方形ガラス基板(一辺の長さが5cmで、厚さが1.0mmのもの)の表面に幅300μm、深さ300μmの溝をピッチ1mmで格子状に形成することで、ガラスモールドを作製する。
次に、このガラスモールドにおける溝が設けられた側の表面に、撥水スプレーで表面コートを施してから液状PDMSプレポリマーを載せる。そして、60℃で3時間加温することで同プレポリマーを固化させ、固化により形成されたPDMS雄型基板(1次PDMS基板)をガラス基板から剥離する。
次に、シリカガラスからなる可撓性角型キャピラリー(商品名:Square flexible fused silica capillary tubing、米国Polymicro社が販売)を用意する。この角型キャピラリーは、外形の横断面形状および内腔の横断面形状がともに正方形であって、外形の幅・高さ(正方形の一辺の長さ)が300μm、内腔の幅・高さが100μmである。
カルシウムイオン認識分子、脂溶性蛍光色素、ポリ塩化ビニル、可塑剤(膜溶媒)(ニトロフェニルオクチルエーテル)をそれぞれ、1.8mg、3.0mg、33.1mg、65.7mgの割合で混合し、250mgのテトラヒドロフランに溶解させる。
角型キャピラリーの内腔にコーティング剤(トリメトキシシリルプロピルメタクリレート−0.1M 塩酸=4:1(vol/vol)を注入し、30分間放置する。その後、メタノールでキャピラリーの内腔を洗浄する。
Claims (8)
- 内部に貫通状流路が形成され、その流路の少なくとも一部にキャピラリーが埋設されるとともに流路閉鎖用のダミーロッドがさらに埋設されてなり、流路は、分枝状または格子状に設けられ、キャピラリーは、ガラスまたはプラスチックからなることを特徴とする微小流路デバイス。
- キャピラリーは、その内部が化学修飾により化学操作部にされている請求項1に記載の微小流路デバイス。
- 微小流路デバイスは、少なくともその一面が透明である請求項1または2に記載の微小流路デバイス。
- 微小流路デバイスは、基板と前記キャピラリーとカバーとから構成され、その基板の表面に流路形成用の溝が設けられ、この溝に前記キャピラリーが密接状に埋設され、溝が設けられた側の基板の表面が前記カバーで被覆されている請求項1〜3のいずれか1つに記載の微小流路デバイス。
- 流路およびキャピラリーは、横断面形状が略方形である請求項1〜4のいずれか1つに記載の微小流路デバイス。
- 基板は、シリコンゴム、ガラスまたはプラスチックからなり、カバーは、ガラスまたはプラスチックの透明シートまたは透明フィルムからなる請求項4または5に記載の微小流路デバイス。
- モールド用基板の表面に溝を設け、この溝が設けられた側のモールド用基板の表面に液状プレポリマーを載せて加温固化させ、固化により形成された雄型基板をモールド用基板から剥離し、この雄型基板の雄型表面に液状プレポリマーを載せて加温固化させ、固化により形成された溝付きの雌型基板を雄型基板から剥離し、この雌型基板の溝の所要箇所にガラスまたはプラスチックからなる分枝状または格子状流路形成用のキャピラリーと流路閉鎖用のダミーロッドとを溝に対して密接状に埋設し、その後、雌型基板の雌型表面に液状プレポリマーを介して透明カバーを載せ、同プレポリマーを加温固化させることからなる微小流路デバイスの作製方法。
- 樹脂型または金型を用いた液状プレポリマーモールディングまたは樹脂射出成形により、溝が形成された基板を作製し、次いで、この基板の溝の所要箇所に内部があらかじめ化学修飾されたガラスまたはプラスチックからなる分枝状または格子状流路形成用のキャピラリーと流路閉鎖用のダミーロッドとを埋設し、その後、溝が設けられた側の基板の表面をガラスまたはプラスチックの透明シートまたは透明フィルムで被覆することからなる微小流路デバイスの作製方法。
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Cited By (2)
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US9372133B2 (en) | 2010-06-30 | 2016-06-21 | Metaboscreen Co., Ltd. | Microchemical chip, producing method thereof and method for using the microchemical chip |
KR20180128054A (ko) | 2016-04-07 | 2018-11-30 | 가부시키가이샤 메타보스크린 | 서모 사이클링 검사 장치 및 칩 홀더 |
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US20060264779A1 (en) | 2005-05-09 | 2006-11-23 | Kemp Timothy M | Fluidic medical devices and uses thereof |
JP5000861B2 (ja) * | 2005-06-17 | 2012-08-15 | 凸版印刷株式会社 | 反応チップ |
JP3116709U (ja) | 2005-09-13 | 2005-12-15 | 有限会社メタボスクリーン | 微小流路チップ |
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US9229001B2 (en) | 2009-11-23 | 2016-01-05 | Cyvek, Inc. | Method and apparatus for performing assays |
JP5616309B2 (ja) | 2010-12-01 | 2014-10-29 | アークレイ株式会社 | デバイス及びその製造方法 |
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JP3419691B2 (ja) * | 1998-09-04 | 2003-06-23 | 日本電信電話株式会社 | 極微少量フローセル、及びその製造方法 |
JP2000093816A (ja) * | 1998-09-26 | 2000-04-04 | Hoya Corp | 小型実験プレートの製造方法及びインライン型小型実験プレートの製造装置 |
WO2002023305A2 (en) * | 2000-09-13 | 2002-03-21 | Bos - Better Online Solution Ltd. | Improved quality of service for internet telephony |
US6981522B2 (en) * | 2001-06-07 | 2006-01-03 | Nanostream, Inc. | Microfluidic devices with distributing inputs |
US6887359B2 (en) * | 2001-06-08 | 2005-05-03 | The Regents Of The University Of California | Chemical micro-sensor |
JP2003270230A (ja) * | 2002-03-15 | 2003-09-25 | Asahi Kasei Corp | 高分子固定化素子 |
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2003
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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US9372133B2 (en) | 2010-06-30 | 2016-06-21 | Metaboscreen Co., Ltd. | Microchemical chip, producing method thereof and method for using the microchemical chip |
KR20180128054A (ko) | 2016-04-07 | 2018-11-30 | 가부시키가이샤 메타보스크린 | 서모 사이클링 검사 장치 및 칩 홀더 |
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