JP5008784B2 - ポリペプチド操作のための方法および組成物 - Google Patents
ポリペプチド操作のための方法および組成物 Download PDFInfo
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- C12N2795/00—Bacteriophages
- C12N2795/00011—Details
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- C12N2795/14111—Inoviridae
- C12N2795/14141—Use of virus, viral particle or viral elements as a vector
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| US08/769,062 US6335160B1 (en) | 1995-02-17 | 1996-12-18 | Methods and compositions for polypeptide engineering |
| US08/769,062 | 1996-12-18 | ||
| PCT/US1997/024239 WO1998027230A1 (en) | 1996-12-18 | 1997-12-17 | Methods and compositions for polypeptide engineering |
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| JP2008306897A Division JP2009089710A (ja) | 1996-12-18 | 2008-12-01 | ポリペプチド操作のための方法および組成物 |
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| JP5008784B2 true JP5008784B2 (ja) | 2012-08-22 |
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| JP2008306897A Withdrawn JP2009089710A (ja) | 1996-12-18 | 2008-12-01 | ポリペプチド操作のための方法および組成物 |
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| JP2008306897A Withdrawn JP2009089710A (ja) | 1996-12-18 | 2008-12-01 | ポリペプチド操作のための方法および組成物 |
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| EP (5) | EP1231269A1 (enExample) |
| JP (2) | JP5008784B2 (enExample) |
| KR (1) | KR20000069591A (enExample) |
| AT (1) | ATE481480T1 (enExample) |
| AU (1) | AU732146B2 (enExample) |
| CA (2) | CA2589337A1 (enExample) |
| DE (1) | DE69739996D1 (enExample) |
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Families Citing this family (461)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7150982B2 (en) | 1991-09-09 | 2006-12-19 | Third Wave Technologies, Inc. | RNA detection assays |
| US6759226B1 (en) | 2000-05-24 | 2004-07-06 | Third Wave Technologies, Inc. | Enzymes for the detection of specific nucleic acid sequences |
| US7045289B2 (en) | 1991-09-09 | 2006-05-16 | Third Wave Technologies, Inc. | Detection of RNA Sequences |
| US6309883B1 (en) | 1994-02-17 | 2001-10-30 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US5837458A (en) | 1994-02-17 | 1998-11-17 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US20060257890A1 (en) | 1996-05-20 | 2006-11-16 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US6406855B1 (en) | 1994-02-17 | 2002-06-18 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
| US6335160B1 (en) | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
| US6238884B1 (en) | 1995-12-07 | 2001-05-29 | Diversa Corporation | End selection in directed evolution |
| US6740506B2 (en) | 1995-12-07 | 2004-05-25 | Diversa Corporation | End selection in directed evolution |
| US6537776B1 (en) * | 1999-06-14 | 2003-03-25 | Diversa Corporation | Synthetic ligation reassembly in directed evolution |
| US5830696A (en) | 1996-12-05 | 1998-11-03 | Diversa Corporation | Directed evolution of thermophilic enzymes |
| US6358709B1 (en) | 1995-12-07 | 2002-03-19 | Diversa Corporation | End selection in directed evolution |
| US6764835B2 (en) | 1995-12-07 | 2004-07-20 | Diversa Corporation | Saturation mutageneis in directed evolution |
| US6939689B2 (en) | 1995-12-07 | 2005-09-06 | Diversa Corporation | Exonuclease-mediated nucleic acid reassembly in directed evolution |
| US6713279B1 (en) | 1995-12-07 | 2004-03-30 | Diversa Corporation | Non-stochastic generation of genetic vaccines and enzymes |
| US6242211B1 (en) | 1996-04-24 | 2001-06-05 | Terragen Discovery, Inc. | Methods for generating and screening novel metabolic pathways |
| US7148054B2 (en) | 1997-01-17 | 2006-12-12 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
| US6326204B1 (en) | 1997-01-17 | 2001-12-04 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
| AU743305C (en) | 1997-01-17 | 2006-03-30 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
| GB9701425D0 (en) * | 1997-01-24 | 1997-03-12 | Bioinvent Int Ab | A method for in vitro molecular evolution of protein function |
| US6159687A (en) | 1997-03-18 | 2000-12-12 | Novo Nordisk A/S | Methods for generating recombined polynucleotides |
| US6153410A (en) * | 1997-03-25 | 2000-11-28 | California Institute Of Technology | Recombination of polynucleotide sequences using random or defined primers |
| GB9722131D0 (en) * | 1997-10-20 | 1997-12-17 | Medical Res Council | Method |
| US6399383B1 (en) | 1997-10-28 | 2002-06-04 | Maxygen, Inc. | Human papilloma virus vectors |
| AU1449499A (en) * | 1997-10-31 | 1999-05-24 | Maxygen, Incorporated | Modification of virus tropism and host range by viral genome shuffling |
| IL136574A0 (en) | 1997-12-08 | 2001-06-14 | California Inst Of Techn | A method for forming a polynucleotide of desired properties |
| US6426185B1 (en) | 1998-01-16 | 2002-07-30 | Large Scale Biology Corporation | Method of compiling a functional gene profile in a plant by transfecting a nucleic acid sequence of a donor plant into a different host plant in an anti-sense orientation |
| US6303848B1 (en) | 1998-01-16 | 2001-10-16 | Large Scale Biology Corporation | Method for conferring herbicide, pest, or disease resistance in plant hosts |
| US6468745B1 (en) | 1998-01-16 | 2002-10-22 | Large Scale Biology Corporation | Method for expressing a library of nucleic acid sequence variants and selecting desired traits |
| US6759243B2 (en) * | 1998-01-20 | 2004-07-06 | Board Of Trustees Of The University Of Illinois | High affinity TCR proteins and methods |
| EP1060395B1 (en) | 1998-02-04 | 2008-04-30 | Invitrogen Corporation | Microarrays and uses therefor |
| US7390619B1 (en) * | 1998-02-11 | 2008-06-24 | Maxygen, Inc. | Optimization of immunomodulatory properties of genetic vaccines |
| JP4540846B2 (ja) * | 1998-04-30 | 2010-09-08 | マックス−プランク−ゲゼルシャフト・ツア・フェルデルング・デア・ヴィッセンシャフテン・エー・ファオ | 所望の生物学的特性を与えるクローンを発現ライブラリーから同定する新規方法 |
| EP1073670A1 (en) | 1998-05-01 | 2001-02-07 | Maxygen, Inc. | Optimization of pest resistance genes using dna shuffling |
| US6902918B1 (en) | 1998-05-21 | 2005-06-07 | California Institute Of Technology | Oxygenase enzymes and screening method |
| US7153655B2 (en) * | 1998-06-16 | 2006-12-26 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence |
| AU4441699A (en) | 1998-06-17 | 2000-01-05 | Maxygen, Inc. | Method for producing polynucleotides with desired properties |
| US6846655B1 (en) | 1998-06-29 | 2005-01-25 | Phylos, Inc. | Methods for generating highly diverse libraries |
| FR2782323B1 (fr) * | 1998-08-12 | 2002-01-11 | Proteus | Procede de production in vitro de sequences polynucleotidiques recombinees, banques de sequences et sequences ainsi obtenues |
| US6951719B1 (en) | 1999-08-11 | 2005-10-04 | Proteus S.A. | Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained |
| US6991922B2 (en) * | 1998-08-12 | 2006-01-31 | Proteus S.A. | Process for in vitro creation of recombinant polynucleotide sequences by oriented ligation |
| US20030092023A1 (en) * | 1998-08-12 | 2003-05-15 | Daniel Dupret | Method of shuffling polynucleotides using templates |
| US20020058249A1 (en) * | 1998-08-12 | 2002-05-16 | Venkiteswaran Subramanian | Dna shuffling to produce herbicide selective crops |
| US20040191772A1 (en) * | 1998-08-12 | 2004-09-30 | Dupret Daniel Marc | Method of shuffling polynucleotides using templates |
| US20030104417A1 (en) * | 1998-08-12 | 2003-06-05 | Proteus S.A. | Template-mediated, ligation-oriented method of nonrandomly shuffling polynucleotides |
| US6605430B1 (en) | 1998-08-12 | 2003-08-12 | Maxygen, Inc. | DNA shuffling of monooxygenase genes for production of industrial chemicals |
| JP2002526107A (ja) * | 1998-10-07 | 2002-08-20 | マキシジェン, インコーポレイテッド | マイコトキシンの解毒のための核酸を生成するためのdnaシャッフリング |
| EP1129184A1 (en) | 1998-11-10 | 2001-09-05 | Maxygen, Inc. | Modified adp-glucose pyrophosphorylase for improvement and optimation of plant phenotypes |
| US20040005673A1 (en) * | 2001-06-29 | 2004-01-08 | Kevin Jarrell | System for manipulating nucleic acids |
| US6358712B1 (en) | 1999-01-05 | 2002-03-19 | Trustee Of Boston University | Ordered gene assembly |
| WO2000040715A2 (en) | 1999-01-05 | 2000-07-13 | Trustees Of Boston University | Improved nucleic acid cloning |
| US6436675B1 (en) | 1999-09-28 | 2002-08-20 | Maxygen, Inc. | Use of codon-varied oligonucleotide synthesis for synthetic shuffling |
| US6376246B1 (en) | 1999-02-05 | 2002-04-23 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
| US20030054390A1 (en) * | 1999-01-19 | 2003-03-20 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
| AU2415200A (en) * | 1999-01-18 | 2000-08-01 | Maxygen, Inc. | Methods of populating data structures for use in evolutionary simulations |
| US6917882B2 (en) | 1999-01-19 | 2005-07-12 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
| US6961664B2 (en) | 1999-01-19 | 2005-11-01 | Maxygen | Methods of populating data structures for use in evolutionary simulations |
| US20070065838A1 (en) * | 1999-01-19 | 2007-03-22 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
| US7024312B1 (en) | 1999-01-19 | 2006-04-04 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
| IL137868A0 (en) | 1999-01-19 | 2001-10-31 | Maxygen Inc | Oligonucleotide mediated nucleic acid recombination |
| US20090130718A1 (en) * | 1999-02-04 | 2009-05-21 | Diversa Corporation | Gene site saturation mutagenesis |
| MXPA00009723A (es) * | 1999-02-04 | 2003-09-22 | Diversa Corp | Generacion no estocastica de vacunas y enzimas geneticas. |
| IL144657A0 (en) | 1999-02-11 | 2002-06-30 | Maxygen Inc | High throughput mass spectrometry |
| US6531316B1 (en) | 1999-03-05 | 2003-03-11 | Maxyag, Inc. | Encryption of traits using split gene sequences and engineered genetic elements |
| IL145165A0 (en) * | 1999-03-09 | 2002-06-30 | Diversa Corp | End selection in directed evolution |
| AU5557400A (en) * | 1999-03-18 | 2001-01-31 | Complete Genomics As | Methods of cloning and producing fragment chains with readable information content |
| AU2005225057A1 (en) * | 1999-03-26 | 2005-12-01 | Bp Corporation North America Inc. | Synthetic ligation reassembly in directed evolution |
| WO2000061740A1 (en) * | 1999-04-10 | 2000-10-19 | Maxygen, Inc. | Modified lipid production |
| AU5805500A (en) * | 1999-07-07 | 2001-01-30 | Maxygen Aps | A method for preparing modified polypeptides |
| ES2257303T3 (es) * | 1999-07-12 | 2006-08-01 | Genentech, Inc. | Vectores de expresion y procedimientos. |
| PT1200606E (pt) | 1999-08-12 | 2006-12-29 | Pfizer Prod Inc | Gene de streptomyces avermitilis que regula a proporção de avermectinas b2:b1 |
| WO2001012791A1 (en) * | 1999-08-12 | 2001-02-22 | Maxygen, Inc. | Dna shuffling of dioxygenase genes for production of industrial chemicals |
| US20040002474A1 (en) * | 1999-10-07 | 2004-01-01 | Maxygen Inc. | IFN-alpha homologues |
| US7430477B2 (en) | 1999-10-12 | 2008-09-30 | Maxygen, Inc. | Methods of populating data structures for use in evolutionary simulations |
| AU1098001A (en) * | 1999-10-19 | 2001-04-30 | Enchira Biotechnology Corporation | Method for directed evolution by random chimeragenesis on transient templates |
| WO2001029212A1 (en) * | 1999-10-19 | 2001-04-26 | Enchira Biotechnology Corporation | Methods for chimeragenesis of whole genomes or large polynucleotides |
| AU1456101A (en) * | 1999-11-03 | 2001-05-14 | Maxygen, Inc. | Antibody diversity generation |
| US6686515B1 (en) | 1999-11-23 | 2004-02-03 | Maxygen, Inc. | Homologous recombination in plants |
| US7115712B1 (en) | 1999-12-02 | 2006-10-03 | Maxygen, Inc. | Cytokine polypeptides |
| CA2395715A1 (en) * | 1999-12-27 | 2001-07-05 | Atsushi Sato | Cytokine-like peptide |
| SG121902A1 (en) | 2000-01-11 | 2006-05-26 | Maxygen Inc | Integrated systems for diversity generation and screening |
| WO2001064864A2 (en) * | 2000-02-28 | 2001-09-07 | Maxygen, Inc. | Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation |
| AU5951201A (en) | 2000-05-04 | 2001-11-12 | Univ Yale | High density protein arrays for screening of protein activity |
| US6479262B1 (en) | 2000-05-16 | 2002-11-12 | Hercules, Incorporated | Solid phase enzymatic assembly of polynucleotides |
| AU2001268716A1 (en) | 2000-06-23 | 2002-01-08 | Maxygen, Inc. | Novel chimeric promoters |
| EP1360290A2 (en) | 2000-06-23 | 2003-11-12 | Maxygen, Inc. | Co-stimulatory molecules |
| US7846733B2 (en) * | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
| US6994963B1 (en) | 2000-07-10 | 2006-02-07 | Ambion, Inc. | Methods for recombinatorial nucleic acid synthesis |
| US6858422B2 (en) | 2000-07-13 | 2005-02-22 | Codexis, Inc. | Lipase genes |
| WO2002006469A2 (en) * | 2000-07-18 | 2002-01-24 | Enchira Biotechnology Corporation | Methods of ligation mediated chimeragenesis utilizing populations of scaffold and donor nucleic acids |
| US20030017552A1 (en) * | 2000-07-21 | 2003-01-23 | Jarrell Kevin A. | Modular vector systems |
| US7435562B2 (en) * | 2000-07-21 | 2008-10-14 | Modular Genetics, Inc. | Modular vector systems |
| WO2002010183A1 (en) | 2000-07-31 | 2002-02-07 | Menzel, Rolf | Compositions and methods for directed gene assembly |
| AUPQ974900A0 (en) * | 2000-08-29 | 2000-09-21 | Macquarie Research Limited | Degenerate oligonucleotide gene-shuffling |
| AU2002246632B2 (en) | 2000-12-08 | 2007-04-05 | Alexion Pharmaceuticals, Inc. | Chronic lymphocytic leukemia cell line and its use for producing an antibody |
| US20060057651A1 (en) | 2000-12-08 | 2006-03-16 | Bowdish Katherine S | Polypeptides and antibodies derived from chronic lymphocytic leukemia cells and uses thereof |
| US7408041B2 (en) | 2000-12-08 | 2008-08-05 | Alexion Pharmaceuticals, Inc. | Polypeptides and antibodies derived from chronic lymphocytic leukemia cells and uses thereof |
| US6958213B2 (en) | 2000-12-12 | 2005-10-25 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function |
| EP1427847B1 (en) * | 2000-12-13 | 2010-07-28 | Nugen Technologies, Inc. | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
| US20020086292A1 (en) | 2000-12-22 | 2002-07-04 | Shigeaki Harayama | Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules |
| US20030036641A1 (en) * | 2001-01-31 | 2003-02-20 | Padgett Hal S. | Methods for homology-driven reassembly of nucleic acid sequences |
| US20110111413A1 (en) * | 2001-02-02 | 2011-05-12 | Padgett Hal S | Method of optimizing codon usage through dna shuffling |
| JP2004537280A (ja) * | 2001-03-07 | 2004-12-16 | ゼノジェン コーポレイション | 標的細胞中へのdnaの導入についてのスクリーニング方法 |
| BR0205268A (pt) * | 2001-03-09 | 2004-11-30 | Nugen Technologies Inc | Processos e composições para a mplificação de sequências de rna |
| US7807408B2 (en) * | 2001-03-19 | 2010-10-05 | President & Fellows Of Harvard College | Directed evolution of proteins |
| WO2002074978A2 (en) | 2001-03-19 | 2002-09-26 | President And Fellows Of Harvard College | Nucleic acid shuffling |
| EP1470219A4 (en) | 2001-04-16 | 2005-10-05 | California Inst Of Techn | PEROXIDE MOLDED CYTOCHROME OXYGENASE P450 OXYGENASE VARIANTS |
| CA2444020A1 (en) * | 2001-05-03 | 2002-11-14 | Rensselaer Polytechnic Institute | Novel methods of directed evolution |
| AU2002254872A1 (en) * | 2001-05-10 | 2002-11-18 | Novozymes A/S | A method for producing recombined polynucleotides |
| WO2002092780A2 (en) * | 2001-05-17 | 2002-11-21 | Diversa Corporation | Novel antigen binding molecules for therapeutic, diagnostic, prophylactic, enzymatic, industrial, and agricultural applications, and methods for generating and screening thereof |
| US20030044980A1 (en) * | 2001-06-05 | 2003-03-06 | Gorilla Genomics, Inc. | Methods for low background cloning of DNA using long oligonucleotides |
| WO2003008563A2 (en) | 2001-07-20 | 2003-01-30 | California Institute Of Technology | Improved cytochrome p450 oxygenases |
| US20050130140A1 (en) * | 2001-07-23 | 2005-06-16 | Bovenberg Roelof A.L. | Process for preparing variant polynucleotides |
| DE60220804D1 (de) * | 2001-08-20 | 2007-08-02 | Regenesis Bioremediation Produ | Biosensor für kleinmolekulare analyten |
| WO2003035842A2 (en) * | 2001-10-24 | 2003-05-01 | Dyax Corporation | Hybridization control of sequence variation |
| DE10162728A1 (de) | 2001-12-20 | 2003-07-10 | Henkel Kgaa | Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease |
| DE10162727A1 (de) | 2001-12-20 | 2003-07-10 | Henkel Kgaa | Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease |
| DE10163884A1 (de) | 2001-12-22 | 2003-07-10 | Henkel Kgaa | Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease |
| US20040009498A1 (en) * | 2002-01-14 | 2004-01-15 | Diversa Corporation | Chimeric antigen binding molecules and methods for making and using them |
| US20040009937A1 (en) * | 2002-01-31 | 2004-01-15 | Wei Chen | Methods and composition for delivering nucleic acids and/or proteins to the respiratory system |
| US20050075298A1 (en) * | 2002-01-31 | 2005-04-07 | Wei Chen | Methods and composition for delivering nucleic acids and/or proteins to the intestinal mucosa |
| US20040043003A1 (en) * | 2002-01-31 | 2004-03-04 | Wei Chen | Clinical grade vectors based on natural microflora for use in delivering therapeutic compositions |
| US20030166010A1 (en) * | 2002-02-25 | 2003-09-04 | Affholter Joseph A. | Custom ligand design for biomolecular filtration and purification for bioseperation |
| ES2564570T3 (es) | 2002-03-01 | 2016-03-23 | Codexis Mayflower Holdings, Llc | Métodos, sistemas y software para la identificación de biomoléculas funcionales |
| US20050084907A1 (en) | 2002-03-01 | 2005-04-21 | Maxygen, Inc. | Methods, systems, and software for identifying functional biomolecules |
| US7747391B2 (en) * | 2002-03-01 | 2010-06-29 | Maxygen, Inc. | Methods, systems, and software for identifying functional biomolecules |
| EP1488335A4 (en) | 2002-03-09 | 2006-11-15 | Maxygen Inc | OPTIMIZING CROSSOVER POINTS FOR THE ADJUSTED EVOLUTION |
| AU2003222269A1 (en) * | 2002-03-11 | 2003-09-29 | Nugen Technologies, Inc. | Methods for generating double stranded dna comprising a 3' single stranded portion and uses of these complexes for recombination |
| US7226771B2 (en) | 2002-04-19 | 2007-06-05 | Diversa Corporation | Phospholipases, nucleic acids encoding them and methods for making and using them |
| BRPI0309391B1 (pt) | 2002-04-19 | 2018-10-30 | Dsm Food Specialties B V | vetor compreendendo ácido nucléico que codifica polipeptídeo com atividade de fosfolipase, célula transformada, preparado de proteína, método de preparação e métodos relacionados |
| CA2485506C (en) * | 2002-05-17 | 2012-02-28 | Alligator Bioscience Ab | A method for in vitro molecular evolution of protein function |
| US9321832B2 (en) | 2002-06-28 | 2016-04-26 | Domantis Limited | Ligand |
| MXPA05001287A (es) | 2002-08-06 | 2005-08-03 | Verdia Llc | Variantes de amino oxidasa ap1. |
| WO2004022593A2 (en) * | 2002-09-09 | 2004-03-18 | Nautilus Biotech | Rational evolution of cytokines for higher stability, the cytokines and encoding nucleic acid molecules |
| US7563600B2 (en) * | 2002-09-12 | 2009-07-21 | Combimatrix Corporation | Microarray synthesis and assembly of gene-length polynucleotides |
| US20060024289A1 (en) * | 2002-10-02 | 2006-02-02 | Ruggles Sandra W | Cleavage of VEGF and VEGF receptor by wild-type and mutant proteases |
| CN104630192A (zh) * | 2002-10-02 | 2015-05-20 | 催化剂生物科学有限公司 | 制备与筛选改变特异性蛋白酶的方法 |
| SI2301965T1 (sl) | 2002-10-16 | 2015-07-31 | Purdue Pharma L.P. | Protitelesa, ki vežejo celično- povezan CA 125 / O722P in metode uporabe le-teh |
| AU2003298594A1 (en) * | 2002-11-01 | 2004-06-07 | Promega Corporation | Cell lysis compositions, methods of use, apparatus, and kit |
| US7314613B2 (en) * | 2002-11-18 | 2008-01-01 | Maxygen, Inc. | Interferon-alpha polypeptides and conjugates |
| AU2003297285A1 (en) * | 2002-11-18 | 2004-06-15 | Maxygen, Inc. | Interferon-alpha polypeptides and conjugates |
| US20040121305A1 (en) * | 2002-12-18 | 2004-06-24 | Wiegand Roger Charles | Generation of efficacy, toxicity and disease signatures and methods of use thereof |
| PL3023498T3 (pl) | 2003-03-06 | 2019-05-31 | Basf Enzymes Llc | Amylazy, kodujące je kwasy nukleinowe i sposoby ich wytwarzania i zastosowania |
| AU2004277368B2 (en) | 2003-03-07 | 2011-01-20 | Dsm Ip Assets B.V. | Hydrolases, nucleic acids encoding them and mehods for making and using them |
| WO2004090099A2 (en) | 2003-04-04 | 2004-10-21 | Diversa Corporation | Pectate lyases, nucleic acids encoding them and methods for making and using them |
| EP1613778B1 (en) * | 2003-04-14 | 2014-11-26 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
| CA2521284C (en) | 2003-04-29 | 2014-07-08 | Pioneer Hi-Bred International, Inc. | Novel glyphosate-n-acetyltransferase (gat) genes |
| US20040248323A1 (en) | 2003-06-09 | 2004-12-09 | Protometrix, Inc. | Methods for conducting assays for enzyme activity on protein microarrays |
| WO2005017105A2 (en) | 2003-06-17 | 2005-02-24 | California University Of Technology | Regio- and enantioselective alkane hydroxylation with modified cytochrome p450 |
| US8603949B2 (en) | 2003-06-17 | 2013-12-10 | California Institute Of Technology | Libraries of optimized cytochrome P450 enzymes and the optimized P450 enzymes |
| DK1641910T3 (da) | 2003-07-02 | 2013-05-21 | Verenium Corp | Glucanaser, nucleinsyrer, som koder for disse, og fremgangsmåder til at fremstille og benytte disse |
| DK1660646T3 (en) | 2003-08-11 | 2015-03-09 | California Inst Of Techn | Thermostable peroxide-driven cytochrome P450 oxygenase variants and methods of use |
| WO2005021714A2 (en) | 2003-08-11 | 2005-03-10 | Diversa Corporation | Laccases, nucleic acids encoding them and methods for making and using them |
| JP4809767B2 (ja) * | 2003-08-18 | 2011-11-09 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | 発現ベクター、ポリペプチドディスプレイライブラリ、並びにそれらの作製及び使用方法 |
| PL1673457T3 (pl) | 2003-08-25 | 2011-12-30 | Amyra Biotech Ag | Nowe białka grzybów oraz kodujące je kwasy nukleinowe |
| WO2005021719A2 (en) * | 2003-08-27 | 2005-03-10 | Proterec Ltd | Libraries of recombinant chimeric proteins |
| KR20070012779A (ko) * | 2003-10-29 | 2007-01-29 | 리보메드 바이오테그놀로지스 인코포레이티드 | 반복적 올리고뉴클레오티드 합성을 위한 조성물, 방법 및검출 기법 |
| CN101671680B (zh) | 2004-02-25 | 2012-11-28 | 先锋高级育种国际公司 | 新的苏云金芽孢杆菌晶体多肽、多核苷酸及其组合物 |
| JP5629423B2 (ja) * | 2004-04-12 | 2014-11-19 | カタリスト バイオサイエンシーズ, インコーポレイテッド | 野生型及び変異型mt−sp1による、vegf及びvegf受容体の切断 |
| BRPI0511196A (pt) * | 2004-05-19 | 2007-12-04 | Maxygen Inc | polipeptìdeo isolado ou recombinante, conjugado, composição, célula hospedeira, vetor, métodos para preparar um polipeptìdeo, para preparar um conjugado, para reduzir o número de cópias de um vìrus em células infectadas com o vìrus, para reduzir o nìvel de rna hcv, dna hbv e rna hiv no soro de um paciente infectado com hcv, hbv e hiv, e, uso do polipeptìdeo, do conjugado ou da composição |
| CA2735315A1 (en) | 2004-06-09 | 2005-12-29 | Pioneer Hi-Bred International, Inc. | Plastid transit peptides |
| CA2570872A1 (en) | 2004-06-16 | 2006-01-26 | Diversa Corporation | Compositions and methods for enzymatic decolorization of chlorophyll |
| CA2572751A1 (en) * | 2004-06-30 | 2006-01-12 | Egen Corporation | Pegylated interferon alpha-1b |
| GB0422052D0 (en) | 2004-10-04 | 2004-11-03 | Dansico As | Enzymes |
| GB0423139D0 (en) * | 2004-10-18 | 2004-11-17 | Danisco | Enzymes |
| US20070122817A1 (en) * | 2005-02-28 | 2007-05-31 | George Church | Methods for assembly of high fidelity synthetic polynucleotides |
| CA2584984A1 (en) * | 2004-10-18 | 2006-04-27 | Codon Devices, Inc. | Methods for assembly of high fidelity synthetic polynucleotides |
| WO2006045503A1 (en) | 2004-10-19 | 2006-05-04 | Lonza Ag | Method for solid phase peptide synthesis |
| JP2008519602A (ja) * | 2004-11-11 | 2008-06-12 | モジュラー ジェネティクス, インコーポレイテッド | 多様性を生じるためのラダーアセンブリおよびシステム |
| WO2006062971A2 (en) * | 2004-12-08 | 2006-06-15 | Ceres Inc. | Modulating plant carbon levels |
| WO2007087601A2 (en) * | 2006-01-25 | 2007-08-02 | Ceres, Inc. | Modulating plant protein levels |
| WO2008076922A1 (en) * | 2006-12-15 | 2008-06-26 | Ceres, Inc. | Modulation of plant protein levels |
| US7335510B2 (en) * | 2004-12-16 | 2008-02-26 | Ceres, Inc. | Modulating plant nitrogen levels |
| JP2008526259A (ja) * | 2005-01-13 | 2008-07-24 | コドン デバイシズ インコーポレイテッド | 蛋白質デザインのための組成物及び方法 |
| US20090038023A1 (en) | 2005-03-10 | 2009-02-05 | Verenium Corporation | Lyase Enzymes, Nucleic Acids Encoding Them and Methods For Making and Using Them |
| KR101393679B1 (ko) | 2005-03-15 | 2014-05-21 | 비피 코포레이션 노쓰 아메리카 인코포레이티드 | 셀룰라제, 이것을 암호화하는 핵산 및 이들을 제조 및사용하는 방법 |
| US11214817B2 (en) | 2005-03-28 | 2022-01-04 | California Institute Of Technology | Alkane oxidation by modified hydroxylases |
| US8715988B2 (en) | 2005-03-28 | 2014-05-06 | California Institute Of Technology | Alkane oxidation by modified hydroxylases |
| US7619067B2 (en) * | 2005-05-18 | 2009-11-17 | Maxygen, Inc. | Evolved interferon-alpha polypeptides |
| US20060281113A1 (en) * | 2005-05-18 | 2006-12-14 | George Church | Accessible polynucleotide libraries and methods of use thereof |
| ES2335232T3 (es) | 2005-06-21 | 2010-03-23 | Xoma Technology Ltd. | Anticuerpos y fragmentos de los mismos que se unen a la il-1 beta. |
| US20070184487A1 (en) * | 2005-07-12 | 2007-08-09 | Baynes Brian M | Compositions and methods for design of non-immunogenic proteins |
| EP1902126A1 (en) * | 2005-07-12 | 2008-03-26 | Codon Devices, Inc | Compositions and methods for biocatalytic engineering |
| EP3896076A1 (en) | 2005-08-31 | 2021-10-20 | The Regents of the University of California | Cellular libraries of peptide sequences (clips) and methods of using the same |
| WO2007030759A2 (en) * | 2005-09-07 | 2007-03-15 | Nugen Technologies, Inc. | Improved nucleic acid amplification procedure |
| GB2432366B (en) * | 2005-11-19 | 2007-11-21 | Alligator Bioscience Ab | A method for in vitro molecular evolution of protein function |
| KR100759817B1 (ko) * | 2005-12-08 | 2007-09-20 | 한국전자통신연구원 | 다중 전사 인자 조절 관계 예측 방법 및 장치 |
| KR20140091765A (ko) * | 2006-01-12 | 2014-07-22 | 알렉시온 파마슈티칼스, 인코포레이티드 | Ox-2/cd200에 대한 항체 및 이들의 용도 |
| US8222482B2 (en) * | 2006-01-26 | 2012-07-17 | Ceres, Inc. | Modulating plant oil levels |
| US20090324574A1 (en) | 2006-02-02 | 2009-12-31 | Verenium Corporation | Esterases and Related Nucleic Acids and Methods |
| CA2637707A1 (en) | 2006-02-02 | 2007-08-09 | Rinat Neuroscience Corporation | Methods for treating obesity by administering a trkb antagonist |
| AU2006338208B2 (en) | 2006-02-10 | 2013-10-24 | Bp Corporation North America Inc. | Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them |
| EP2548954A1 (en) | 2006-02-14 | 2013-01-23 | Verenium Corporation | Xylanases, nucleic acids encoding them and methods for making and using them |
| KR101744157B1 (ko) | 2006-03-07 | 2017-06-08 | 바스프 엔자임스 엘엘씨 | 알돌라제, 이것을 코딩하는 핵산과 그 제조 방법 및 사용 방법 |
| CN101437954B (zh) | 2006-03-07 | 2015-09-09 | 嘉吉公司 | 醛缩酶和编码醛缩酶的核酸以及它们的制备和使用方法 |
| US20070231805A1 (en) * | 2006-03-31 | 2007-10-04 | Baynes Brian M | Nucleic acid assembly optimization using clamped mismatch binding proteins |
| US20100024070A1 (en) * | 2006-05-15 | 2010-01-28 | Steven Craig Bobzin | Modulation of oil levels in plants |
| WO2007136834A2 (en) * | 2006-05-19 | 2007-11-29 | Codon Devices, Inc. | Combined extension and ligation for nucleic acid assembly |
| AR061365A1 (es) * | 2006-06-13 | 2008-08-20 | Athenix Corp | Metodos para generar diversidad genetica mediante mutagenesis con permutaciones |
| WO2007149570A2 (en) * | 2006-06-21 | 2007-12-27 | Ceres, Inc. | Modulation of protein levels in plants |
| US7572618B2 (en) | 2006-06-30 | 2009-08-11 | Bristol-Myers Squibb Company | Polynucleotides encoding novel PCSK9 variants |
| US8252559B2 (en) | 2006-08-04 | 2012-08-28 | The California Institute Of Technology | Methods and systems for selective fluorination of organic molecules |
| DK2069389T3 (en) | 2006-08-04 | 2015-01-12 | Bp Corp North America Inc | Glucanases, nucleic acids encoding them, and processes for their preparation and use |
| US8026085B2 (en) | 2006-08-04 | 2011-09-27 | California Institute Of Technology | Methods and systems for selective fluorination of organic molecules |
| US7727745B2 (en) * | 2006-08-14 | 2010-06-01 | Bio-Id Diagnostic Inc. | Synthesis of single-stranded DNA |
| AU2007234569C1 (en) * | 2006-08-15 | 2008-05-29 | Commonwealth Scientific And Industrial Research Organisation | Reassortment by fragment ligation |
| US8158411B2 (en) | 2006-08-21 | 2012-04-17 | Samsung Electronics Co., Ltd. | Method of separating microorganism using nonplanar solid substrate and device for separating microorganism using the same |
| US8053191B2 (en) | 2006-08-31 | 2011-11-08 | Westend Asset Clearinghouse Company, Llc | Iterative nucleic acid assembly using activation of vector-encoded traits |
| EP2617815B1 (en) | 2006-09-21 | 2015-11-18 | BASF Enzymes LLC | Phytases, nucleic acids encoding them and methods for making and using them |
| ES2451266T3 (es) | 2006-09-21 | 2014-03-26 | Dsm Ip Assets B.V. | Fosfolipasas, ácidos nucleicos que las codifican, y métodos para obtenerlas y usarlas |
| ES2531167T5 (es) | 2006-12-21 | 2018-05-21 | Basf Enzymes Llc | Amilasas y glucoamilasas, ácidos nucleicos que las codifican y métodos para formarlas y utilizarlas |
| DE102007003143A1 (de) | 2007-01-16 | 2008-07-17 | Henkel Kgaa | Neue Alkalische Protease aus Bacillus gibsonii und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease |
| US20080175668A1 (en) * | 2007-01-18 | 2008-07-24 | Larry Wayne Haese | Graywater recycling system including rainwater recovery |
| EP2074136A4 (en) | 2007-01-30 | 2012-11-07 | Verenium Corp | ENZYMES FOR TREATING LIGNOCELLULOSE, FOR SUCH CODING NUCLEIC ACIDS, AND METHOD FOR THE PRODUCTION AND USE THEREOF |
| US8143046B2 (en) | 2007-02-07 | 2012-03-27 | Danisco Us Inc., Genencor Division | Variant Buttiauxella sp. phytases having altered properties |
| US7820421B2 (en) | 2007-02-08 | 2010-10-26 | Codexis, Inc. | Ketoreductases and uses thereof |
| EP2118281A2 (en) | 2007-02-12 | 2009-11-18 | Codexis, Inc. | Structure-activity relationships |
| EP2126087A4 (en) * | 2007-02-20 | 2010-10-27 | Anaptysbio Inc | METHODS FOR PRODUCING PHARMACOTHEQUES, AND USES THEREOF |
| US7625555B2 (en) | 2007-06-18 | 2009-12-01 | Novagen Holding Corporation | Recombinant human interferon-like proteins |
| US8986684B2 (en) | 2007-07-25 | 2015-03-24 | Alexion Pharmaceuticals, Inc. | Methods and compositions for treating autoimmune disease |
| WO2009014726A1 (en) | 2007-07-26 | 2009-01-29 | The Regents Of The University Of California | Methods for enhancing bacterial cell display of proteins and peptides |
| WO2009018449A1 (en) | 2007-07-31 | 2009-02-05 | Verenium Corporation | Tailored multi-site combinatorial assembly |
| WO2009026448A1 (en) * | 2007-08-21 | 2009-02-26 | Affomix Corporation | Interaction screening methods, systems and devices |
| JP2010536385A (ja) | 2007-08-24 | 2010-12-02 | コデクシス, インコーポレイテッド | (r)−3−ヒドロキシチオランの立体選択的生成のための改善されたケトレダクターゼポリペプチド |
| ES2547528T3 (es) | 2007-10-01 | 2015-10-07 | Codexis, Inc. | Polipéptidos de cetorreductasa para la producción de acetidinona |
| MX2010003600A (es) | 2007-10-03 | 2010-12-14 | Verenium Corp | Xilanasas, acidos nucleicos que las codifican, y metodos para hacerlas y usarlas. |
| DE102007051092A1 (de) | 2007-10-24 | 2009-04-30 | Henkel Ag & Co. Kgaa | Subtilisin aus Becillus pumilus und Wasch- und Reinigungsmittel enthaltend dieses neue Subtilisin |
| AU2008319053B2 (en) | 2007-11-01 | 2012-04-26 | Astellas Pharma Inc. | Immunosuppressive polypeptides and nucleic acids |
| DK2238242T5 (en) | 2008-01-03 | 2015-08-03 | Basf Enzymes Llc | -Transferases and oxidoreductases, nucleic acids encoding FOR THEM, AND PROCESSES FOR THE STILL LING AND USE THEREOF |
| DK2238261T3 (da) | 2008-01-03 | 2014-02-10 | Verenium Corp | Isomeraser, nukleinsyrer der koder for dem og fremgangsmåder til fremstilling og anvendelse deraf |
| WO2009097673A1 (en) * | 2008-02-07 | 2009-08-13 | Bio-Id Diagnostic Inc. | Synthesis of single-stranded dna |
| US8034568B2 (en) * | 2008-02-12 | 2011-10-11 | Nugen Technologies, Inc. | Isothermal nucleic acid amplification methods and compositions |
| EP2250594B1 (en) | 2008-02-12 | 2017-04-19 | Codexis, Inc. | Method of generating an optimized, diverse population of variants |
| US8504498B2 (en) | 2008-02-12 | 2013-08-06 | Codexis, Inc. | Method of generating an optimized, diverse population of variants |
| BR122018014094B1 (pt) | 2008-02-15 | 2021-01-05 | Ceres, Inc. | métodos de produção de uma planta dicotiledônea e para aumentar a tolerância ao calor em uma planta dicotiledônea |
| WO2009117698A2 (en) | 2008-03-21 | 2009-09-24 | Nugen Technologies, Inc. | Methods of rna amplification in the presence of dna |
| EP2297310A2 (en) | 2008-05-23 | 2011-03-23 | E. I. du Pont de Nemours and Company | Novel dgat genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants |
| ES2575005T3 (es) | 2008-06-13 | 2016-06-23 | Codexis, Inc. | Método de síntesis de variantes de polinucleótidos |
| US20090312196A1 (en) | 2008-06-13 | 2009-12-17 | Codexis, Inc. | Method of synthesizing polynucleotide variants |
| US8383346B2 (en) | 2008-06-13 | 2013-02-26 | Codexis, Inc. | Combined automated parallel synthesis of polynucleotide variants |
| CN102816106A (zh) | 2008-06-24 | 2012-12-12 | 默沙东公司 | 用于制备基本上立体异构纯的稠合二环脯氨酸化合物的生物催化方法 |
| HUE026181T2 (en) | 2008-08-27 | 2016-05-30 | Codexis Inc | Ketoreductase polypeptide for the preparation of 3-aryl-3-hydroxypropanamine from 3-aryl-3-ketopropanamine |
| US8288141B2 (en) | 2008-08-27 | 2012-10-16 | Codexis, Inc. | Ketoreductase polypeptides for the production of 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine |
| WO2010027710A2 (en) | 2008-08-27 | 2010-03-11 | Codexis, Inc. | Ketoreductase polypeptides and uses thereof |
| US8198062B2 (en) | 2008-08-29 | 2012-06-12 | Dsm Ip Assets B.V. | Hydrolases, nucleic acids encoding them and methods for making and using them |
| US8273554B2 (en) | 2008-08-29 | 2012-09-25 | Codexis, Inc. | Ketoreductase polypeptides for the stereoselective production of (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one |
| EP3502256A3 (en) | 2008-09-26 | 2019-09-25 | Tocagen Inc. | Recombinant vectors |
| US8247192B2 (en) * | 2008-11-10 | 2012-08-21 | Codexis, Inc. | Penicillin-G acylases |
| EP2382308B1 (en) | 2008-12-25 | 2015-03-04 | Codexis, Inc. | Enone reductases |
| ES2840000T3 (es) | 2009-01-08 | 2021-07-06 | Codexis Inc | Polipéptidos de transaminasa |
| US8738296B2 (en) * | 2009-02-03 | 2014-05-27 | Complete Genomics, Inc. | Indexing a reference sequence for oligomer sequence mapping |
| US8731843B2 (en) * | 2009-02-03 | 2014-05-20 | Complete Genomics, Inc. | Oligomer sequences mapping |
| EP2394164A4 (en) * | 2009-02-03 | 2014-01-08 | Complete Genomics Inc | ASSIGNMENT OF OLIGOMER SEQUENCES |
| EP2401369B1 (en) | 2009-02-26 | 2014-12-24 | Codexis, Inc. | Beta-glucosidase variant enzymes and related polynucleotides |
| CN102405281B (zh) * | 2009-02-26 | 2015-05-13 | 科德克希思公司 | 转氨酶生物催化剂 |
| KR20110136825A (ko) * | 2009-03-09 | 2011-12-21 | 바이오아트라, 엘엘씨 | 미락 단백질 |
| BRPI1013886A2 (pt) | 2009-03-17 | 2016-10-11 | Codexis Inc | variantes de endoglucanases, polinucleotídeos e usos a elas relacionados |
| US8088608B2 (en) * | 2009-03-31 | 2012-01-03 | Codexis, Inc. | Endoglucanases |
| US20120100997A1 (en) | 2009-04-24 | 2012-04-26 | University Of Southern California | Cd133 polymorphisms and expression predict clinical outcome in patients with cancer |
| WO2010127045A2 (en) * | 2009-04-29 | 2010-11-04 | Complete Genomics, Inc. | Method and system for calling variations in a sample polynucleotide sequence with respect to a reference polynucleotide sequence |
| WO2010135588A2 (en) | 2009-05-21 | 2010-11-25 | Verenium Corporation | Phytases, nucleic acids encoding them and methods for making and using them |
| EP2443139B1 (en) | 2009-06-16 | 2016-08-10 | Codexis, Inc. | Beta-glucosidase variant enzymes and related polynucleotides |
| BRPI1010696A2 (pt) | 2009-06-16 | 2016-10-11 | Codexis Inc | método de produção de um polipeptídeo de b-glicosidade com termoatividade aperfeiçoada, polipeptídeo, proteína e seus usos |
| DK3409765T3 (da) | 2009-06-22 | 2021-09-27 | Codexis Inc | Ketoreduktase-medieret stereoselektiv rute til alpha-chloralkoholer |
| US8921079B2 (en) | 2009-06-22 | 2014-12-30 | Codexis, Inc. | Transaminase reactions |
| US8614081B2 (en) | 2009-07-23 | 2013-12-24 | Codexis, Inc. | Nitrilase biocatalysts |
| ES2575560T3 (es) | 2009-08-19 | 2016-06-29 | Codexis, Inc. | Polipéptidos cetorreductasa para preparar fenilefrina |
| CA2770854A1 (en) | 2009-08-20 | 2011-02-24 | Pioneer Hi-Bred International, Inc. | Functional expression of shuffled yeast nitrate transporter (ynt1) in maize to improve nitrate uptake under low nitrate environment |
| EP2473604B1 (en) | 2009-09-04 | 2017-01-11 | Codexis, Inc. | Variant cbh2 cellulases and related polynucleotides |
| WO2011035105A1 (en) | 2009-09-18 | 2011-03-24 | Codexis, Inc. | Reduced codon mutagenesis |
| WO2011041594A1 (en) | 2009-09-30 | 2011-04-07 | Codexis, Inc. | Recombinant c1 b-glucosidase for production of sugars from cellulosic biomass |
| UA111708C2 (uk) | 2009-10-16 | 2016-06-10 | Бандж Ойлз, Інк. | Спосіб рафінування олії |
| UA109884C2 (uk) | 2009-10-16 | 2015-10-26 | Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування | |
| CA2780974C (en) | 2009-11-20 | 2016-02-02 | Codexis, Inc. | Mutli-cellulase enzyme compositions for hydrolysis of cellulosic biomass |
| CA2781399C (en) | 2009-11-25 | 2015-01-20 | Codexis, Inc. | Recombinant thermoascus aurantiacus beta-glucosidase variants for production of fermentable sugars from cellulosic biomass |
| PL2504017T3 (pl) | 2009-11-25 | 2014-06-30 | Codexis Inc | Rekombinacyjne warianty beta-glukozydazy do wytwarzania rozpuszczalnych cukrów z biomasy celulozowej |
| EP2510089B1 (en) | 2009-12-08 | 2015-10-21 | Codexis, Inc. | Synthesis of prazole compounds |
| US9593326B2 (en) | 2009-12-09 | 2017-03-14 | Codexis, Inc. | Protein variant generation by region shuffling |
| WO2011084757A1 (en) | 2009-12-21 | 2011-07-14 | University Of Southern California | Germline polymorphisms in the sparc gene associated with clinical outcome in gastric cancer |
| CA2786001A1 (en) | 2009-12-31 | 2011-07-07 | Pioneer Hi-Bred International, Inc. | Engineering plant resistance to diseases caused by pathogens |
| WO2011085334A1 (en) | 2010-01-11 | 2011-07-14 | University Of Southern California | Cd44 polymorphisms predict clinical outcome in patients with gastric cancer |
| US9080192B2 (en) | 2010-02-10 | 2015-07-14 | Codexis, Inc. | Processes using amino acid dehydrogenases and ketoreductase-based cofactor regenerating system |
| US8986965B2 (en) | 2010-03-31 | 2015-03-24 | Codexis, Inc. | Production of monoterpenes |
| HUE026367T2 (en) | 2010-05-04 | 2016-06-28 | Codexis Inc | Biocatalysts for ezetimibe synthesis |
| EP2569331A1 (en) | 2010-05-10 | 2013-03-20 | Perseid Therapeutics LLC | Polypeptide inhibitors of vla4 |
| EP2569426A4 (en) | 2010-05-14 | 2013-10-09 | Codexis Inc | ZELLBIOHYDROLASE VARIANTS |
| CA2798937A1 (en) | 2010-05-28 | 2011-12-01 | Codexis, Inc. | Pentose fermentation by a recombinant microorganism |
| EP2582799B1 (en) | 2010-06-17 | 2017-12-20 | Codexis, Inc. | Biocatalysts and methods for the synthesis of (s)-3-(1-aminoethyl)-phenol |
| US8574878B2 (en) | 2010-06-28 | 2013-11-05 | Behnaz Behrouzian | Fatty alcohol forming acyl reductases (FARs) and methods of use thereof |
| EP2590991B1 (en) | 2010-06-30 | 2016-01-20 | Codexis, Inc. | Highly stable beta-class carbonic anhydrases useful in carbon capture systems |
| BR112013003224A2 (pt) | 2010-08-13 | 2016-06-07 | Pioneer Hi Bred Int | "construto de promotor quimérico, cassete de expressão, vetor de expressão, método de obtenção de uma planta, método para regular a expressão de um polinucleotídeo de interesse, polinucleotídeo, método para expressar um polinucleotídeo de interesse" |
| EP2605772B1 (en) | 2010-08-16 | 2016-06-22 | Cardiome International AG | Process for preparing aminocyclohexyl ether compounds |
| US8932836B2 (en) | 2010-08-16 | 2015-01-13 | Codexis, Inc. | Biocatalysts and methods for the synthesis of (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine |
| US9322027B2 (en) | 2010-08-20 | 2016-04-26 | Shell Oil Company | Expression constructs comprising fungal promoters |
| WO2012024698A1 (en) | 2010-08-20 | 2012-02-23 | Codexis, Inc. | Use of glycoside hydrolase 61 family proteins in processing of cellulose |
| ES2604114T3 (es) | 2010-11-02 | 2017-03-03 | Codexis, Inc. | Cepas de hongos mejoradas |
| DK2635690T3 (en) | 2010-11-02 | 2016-02-15 | Codexis Inc | FORMATIONS AND PROCESSES FOR PREPARING fermentable SUGAR |
| EP3705573A1 (en) | 2010-11-12 | 2020-09-09 | Gen9, Inc. | Methods and devices for nucleic acids synthesis |
| US10457935B2 (en) | 2010-11-12 | 2019-10-29 | Gen9, Inc. | Protein arrays and methods of using and making the same |
| DK2649187T3 (da) | 2010-12-08 | 2018-01-29 | Codexis Inc | Biokatalysatorer og fremgangsmåder til syntesen af armodafinil |
| WO2012088159A2 (en) | 2010-12-21 | 2012-06-28 | Codexis, Inc. | Endoglucanase variants |
| WO2012135110A1 (en) | 2011-03-30 | 2012-10-04 | Codexis, Inc. | Pentose fermentation by a recombinant microorganism |
| US20120258866A1 (en) * | 2011-04-05 | 2012-10-11 | Full Spectrum Genetics, Inc. | Multi-dimensional selection of protein mutants using high throughput sequence analysis |
| EP2697387B1 (en) | 2011-04-12 | 2021-09-15 | Bactoclear Holdings PTE. LTD. | Chimeric antibacterial polypeptides |
| HUE038800T2 (hu) | 2011-04-13 | 2018-11-28 | Codexis Inc | Biokatalitikus eljárás eszlikarbazepin és ennek analógjai elõállítására |
| US8778652B2 (en) | 2011-06-30 | 2014-07-15 | Codexis, Inc. | Pentose fermentation by a recombinant microorganism |
| WO2013016207A2 (en) | 2011-07-22 | 2013-01-31 | California Institute Of Technology | Stable fungal cel6 enzyme variants |
| BR112014004171A2 (pt) | 2011-08-22 | 2018-11-06 | Codexis Inc | variantes da proteína glicósideo hidrolase gh61 e cofatores que aumentam a atividade de gh61 |
| EP2748187A4 (en) | 2011-08-23 | 2015-03-04 | Codexis Inc | VARIANTS OF CELLOBIOHYDROLASE |
| DK3594340T3 (da) | 2011-08-26 | 2021-09-20 | Gen9 Inc | Sammensætninger og fremgangsmåder til samling med høj nøjagtighed af nukleinsyrer |
| CN103998461B (zh) | 2011-09-08 | 2018-02-02 | 科德克希思公司 | 用于合成取代的内酰胺的生物催化剂和方法 |
| EP2760997A4 (en) | 2011-09-30 | 2015-02-11 | Codexis Inc | MUSHROOM PROTEASE |
| IN2014CN04470A (enExample) | 2011-11-18 | 2015-09-04 | Codexis Inc | |
| EP2794893A4 (en) | 2011-12-20 | 2015-08-12 | Codexis Inc | PRODUCTION OF SATURATED FATTY ALCOHOLS FROM GENETICALLY MODIFIED MICROORGANISMS |
| EP2794871A4 (en) | 2011-12-20 | 2015-12-30 | Codexis Inc | VARIANTS OF ENDOGLUCANASE-1B (EG1B) |
| US9150853B2 (en) | 2012-03-21 | 2015-10-06 | Gen9, Inc. | Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis |
| DK2828385T3 (en) | 2012-03-23 | 2018-03-12 | Codexis Inc | BIOCATALIZATORS AND METHODS FOR SYNTHETIZING DERIVATIVES AND TRYPTAMIN AND TRYPTAMINE ANALOGS |
| US20150133698A1 (en) | 2012-04-20 | 2015-05-14 | Codexis, Inc. | Production of fatty alcohols from engineered microorganisms |
| AU2013251701A1 (en) | 2012-04-24 | 2014-10-30 | Gen9, Inc. | Methods for sorting nucleic acids and multiplexed preparative in vitro cloning |
| BR112014027468A2 (pt) | 2012-05-04 | 2017-06-27 | Du Pont | polinucleotídeo isolado ou recombinante, construção de dna recombinante, célula, planta, explante vegetal, semente transgênica, polipeptídeo isolado, composição, métodos de produção de meganuclease, de introdução de rompimento e de integração de um polinucleotídeo. |
| SG11201407295YA (en) | 2012-05-08 | 2014-12-30 | Codexis Inc | Biocatalysts and methods for hydroxylation of chemical compounds |
| SG11201407297VA (en) | 2012-05-11 | 2014-12-30 | Codexis Inc | Engineered imine reductases and methods for the reductive amination of ketone and amine compounds |
| CA2873511A1 (en) | 2012-05-17 | 2013-11-21 | Ra Pharmaceuticals, Inc. | Peptide and peptidomimetic inhibitors |
| BR112014031024A2 (pt) | 2012-06-11 | 2017-06-27 | Codexis Inc | xilanases e xilosidases fúngicas. |
| AU2013280661A1 (en) | 2012-06-25 | 2015-01-22 | Gen9, Inc. | Methods for nucleic acid assembly and high throughput sequencing |
| WO2014015278A1 (en) | 2012-07-20 | 2014-01-23 | Codexis, Inc. | Production of fatty alcohols from engineered microorganisms |
| US9506087B2 (en) | 2012-08-07 | 2016-11-29 | Codexis, Inc. | Glucose and xylose co-utilization in E. coli |
| EP3450549B1 (en) | 2012-10-29 | 2020-09-30 | University Of Rochester | Artemisinin derivatives, methods for their preparation and their use as antimalarial agents |
| CA2891482A1 (en) | 2012-11-20 | 2014-05-30 | Codexis, Inc. | Pentose fermentation by a recombinant microorganism |
| ES2672328T3 (es) | 2012-12-07 | 2018-06-13 | Merck Sharp & Dohme Corp. | Proceso de transaminación biocatalítica |
| CN104955961B (zh) * | 2012-12-11 | 2017-03-08 | 塞勒密斯株式会社 | 利用密码子随机化和诱变来合成基因文库的方法 |
| WO2014093070A1 (en) | 2012-12-14 | 2014-06-19 | Codexis, Inc. | Modified native beta-ketoacyl-acp synthases and engineered microorganisms |
| CN120829884A (zh) | 2012-12-21 | 2025-10-24 | 科德克希思公司 | 用于合成手性胺的工程化的生物催化剂和方法 |
| HUE044110T2 (hu) | 2013-01-18 | 2019-09-30 | Codexis Inc | Módosított biokatalizátorok, amelyek alkalmasak karbapeném szintézisére |
| CN105074463B (zh) | 2013-01-31 | 2018-09-25 | 科德克希思公司 | 使用相乘形式的模型鉴定生物分子的方法、系统和软件 |
| SG11201506748WA (en) | 2013-02-28 | 2015-09-29 | Codexis Inc | Engineered transaminase polypeptides for industrial biocatalysis |
| AU2014236162A1 (en) | 2013-03-14 | 2015-09-17 | Arzeda Corp. | Compositions having dicamba decarboxylase activity and methods of use |
| AU2014236154A1 (en) | 2013-03-14 | 2015-09-17 | Pioneer Hi-Bred International, Inc. | Compositions having dicamba decarboxylase activity and methods of use |
| US9670493B2 (en) | 2013-03-14 | 2017-06-06 | Codexis, Inc. | Low-phosphate repressible promoter |
| US10023877B2 (en) | 2013-03-15 | 2018-07-17 | Pioneer Hi-Bred International, Inc. | PHI-4 polypeptides and methods for their use |
| CN105324483B (zh) | 2013-04-18 | 2018-11-30 | 科德克希思公司 | 工程化苯丙氨酸解氨酶多肽 |
| CN106232820A (zh) | 2013-08-16 | 2016-12-14 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
| WO2015027245A1 (en) | 2013-08-23 | 2015-02-26 | Complete Genomics, Inc. | Long fragment de novo assembly using short reads |
| US10667524B2 (en) | 2013-09-13 | 2020-06-02 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| EP3418929B1 (en) | 2013-09-27 | 2020-12-09 | Codexis, Inc. | Automated screening of enzyme variants |
| CN106232619B (zh) | 2013-11-13 | 2022-09-09 | 科德克希思公司 | 工程化的亚胺还原酶和用于酮和胺化合物的还原胺化的方法 |
| WO2015100277A2 (en) | 2013-12-23 | 2015-07-02 | University Of Rochester | Methods and compositions for ribosomal synthesis of macrocyclic peptides |
| CN114763376A (zh) | 2014-02-07 | 2022-07-19 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
| PH12016501534B1 (en) | 2014-02-07 | 2024-06-05 | Du Pont | Insecticidal proteins and methods for their use |
| WO2015145463A1 (en) | 2014-03-27 | 2015-10-01 | Gangagen, Inc. | Phage-derived compositions for improved mycobacterial therapy |
| DK3145536T3 (da) | 2014-04-16 | 2022-01-03 | Codexis Inc | Modificeret tyrosinammoniaklyase |
| WO2016007623A1 (en) | 2014-07-09 | 2016-01-14 | Codexis, Inc. | Novel p450-bm3 variants with improved activity |
| US10526391B2 (en) | 2014-07-22 | 2020-01-07 | The University Of Notre Dame Du Lac | Molecular constructs and uses thereof |
| US10045987B2 (en) | 2014-08-28 | 2018-08-14 | Codexis, Inc. | Imidazoyl anilide derivatives and methods of use |
| US10273229B2 (en) | 2014-08-28 | 2019-04-30 | Codexis, Inc. | N-substituted 4-aminoquinazoline derivatives and methods of use |
| UA126192C2 (uk) | 2014-10-16 | 2022-08-31 | Піонір Хай-Бред Інтернешнл, Інк. | Інсектицидний білок та спосіб його застосування |
| US10370648B2 (en) | 2014-11-25 | 2019-08-06 | Codexis, Inc. | Engineered imine reductases and methods for the reductive amination of ketone and amine compounds |
| WO2016183532A1 (en) | 2015-05-13 | 2016-11-17 | Synlogic, Inc. | Bacteria engineered to treat a disease or disorder |
| US20170360900A1 (en) | 2014-12-22 | 2017-12-21 | Codexis, Inc. | Human alpha-galactosidase variants |
| CN114075267B (zh) | 2015-01-15 | 2025-03-18 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
| US10696953B2 (en) | 2015-02-10 | 2020-06-30 | Codexis, Inc. | Ketoreductase polypeptides for the synthesis of chiral compounds |
| CN107529763B (zh) | 2015-03-11 | 2021-08-20 | 先锋国际良种公司 | Pip-72的杀昆虫组合及使用方法 |
| US9944916B2 (en) | 2015-05-07 | 2018-04-17 | Codexis, Inc. | Penicillin-G acylases |
| CN116333064A (zh) | 2015-05-19 | 2023-06-27 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
| WO2016210384A2 (en) | 2015-06-25 | 2016-12-29 | Synlogic, Inc. | Bacteria engineered to treat metabolic diseases |
| DK3319987T3 (da) | 2015-07-07 | 2021-06-21 | Codexis Inc | Nye p450-bm3-varianter med forbedret aktivitet |
| US11198709B2 (en) | 2015-08-06 | 2021-12-14 | E. I. Du Pont De Nemours And Company | Plant derived insecticidal proteins and methods for their use |
| JP6821598B2 (ja) | 2015-12-07 | 2021-01-27 | ザイマージェン インコーポレイテッド | Corynebacterium glutamicum由来のプロモーター |
| US11208649B2 (en) | 2015-12-07 | 2021-12-28 | Zymergen Inc. | HTP genomic engineering platform |
| US9988624B2 (en) | 2015-12-07 | 2018-06-05 | Zymergen Inc. | Microbial strain improvement by a HTP genomic engineering platform |
| EP3390431A1 (en) | 2015-12-18 | 2018-10-24 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| EP3402497A1 (en) | 2016-01-11 | 2018-11-21 | Synlogic Operating Company, Inc. | Bacteria engineered to treat metabolic diseases |
| WO2017123592A1 (en) | 2016-01-11 | 2017-07-20 | Synlogic, Inc. | Bacteria engineered to treat disorders associated with bile salts |
| WO2017123676A1 (en) | 2016-01-11 | 2017-07-20 | Synlogic, Inc. | Recombinant bacteria engineered to treat diseases and disorders associated with amino acid metabolism and methods of use thereof |
| WO2017139708A1 (en) | 2016-02-10 | 2017-08-17 | Synlogic, Inc. | Bacteria engineered to treat nonalcoholic steatohepatitis (nash) |
| EP3451837B1 (en) | 2016-05-04 | 2021-08-25 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| CN109715793A (zh) | 2016-05-05 | 2019-05-03 | 科德克希思公司 | 青霉素-g 酰化酶 |
| CN109715817B (zh) | 2016-06-09 | 2022-12-09 | 科德克希思公司 | 用于化合物的羟基化的生物催化剂和方法 |
| EP3471744A4 (en) | 2016-06-15 | 2020-07-29 | Codexis, Inc. | REVISED BETA-GLUCOSIDASES AND GLUCOSYLATION PROCESSES |
| JP2019519241A (ja) | 2016-06-30 | 2019-07-11 | ザイマージェン インコーポレイテッド | グルコース透過酵素ライブラリーを生成するための方法およびその使用 |
| US10544390B2 (en) | 2016-06-30 | 2020-01-28 | Zymergen Inc. | Methods for generating a bacterial hemoglobin library and uses thereof |
| MX387506B (es) | 2016-07-01 | 2025-03-18 | Pioneer Hi Bred Int | Proteinas insecticidas de plantas y metodos para sus usos. |
| EP3515926B1 (en) | 2016-08-26 | 2023-10-25 | Codexis, Inc. | Engineered imine reductases and methods for the reductive amination of ketone and amine compounds |
| MX387927B (es) | 2016-11-01 | 2025-03-19 | Pioneer Hi Bred Int | Proteinas insecticidas y metodos para su uso. |
| WO2018111551A1 (en) | 2016-12-14 | 2018-06-21 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| US11213028B2 (en) | 2016-12-22 | 2022-01-04 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| MX2019008096A (es) | 2017-01-05 | 2019-09-04 | Codexis Inc | Penicilina g acilasas. |
| IL321402A (en) | 2017-02-03 | 2025-08-01 | Tate & Lyle Solutions Usa Llc | Engineered glycosyltransferases and steviol glycoside glucosylation methods |
| US20190390219A1 (en) | 2017-02-08 | 2019-12-26 | Pioneer Hi-Bred International, Inc. | Insecticidal combinations of plant derived insecticidal proteins and methods for their use |
| NZ755628A (en) | 2017-02-13 | 2023-09-29 | Codexis Inc | Engineered phenylalanine ammonia lyase polypeptides |
| CN110753684A (zh) | 2017-04-24 | 2020-02-04 | 特沙诺有限公司 | 尼拉帕利的制造方法 |
| SG11201909712TA (en) | 2017-04-27 | 2019-11-28 | Codexis Inc | Ketoreductase polypeptides and polynucleotides |
| CA3062550A1 (en) | 2017-05-08 | 2018-11-15 | Codexis, Inc. | Engineered ligase variants |
| WO2018208882A1 (en) | 2017-05-11 | 2018-11-15 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| EP3638688A4 (en) | 2017-06-14 | 2021-07-07 | Codexis, Inc. | MODIFIED TRANSAMINASE POLYPEPTIDES INTENDED FOR INDUSTRIAL BIOCATALYSIS |
| CA3064577A1 (en) | 2017-06-27 | 2019-01-03 | Codexis, Inc. | Penicillin-g acylases |
| AU2018292104B2 (en) | 2017-06-30 | 2025-03-27 | Codexis, Inc. | T7 RNA polymerase variants |
| JP7588391B2 (ja) | 2017-06-30 | 2024-11-22 | コデクシス, インコーポレイテッド | T7 rnaポリメラーゼバリアント |
| US11634400B2 (en) | 2017-08-19 | 2023-04-25 | University Of Rochester | Micheliolide derivatives, methods for their preparation and their use as anticancer and antiinflammatory agents |
| US11761963B2 (en) | 2017-09-27 | 2023-09-19 | Alexion Pharmaceuticals, Inc. | Biomarker signature for predicting tumor response to anti-CD200 therapy |
| EP3706780A4 (en) | 2017-11-07 | 2021-12-22 | Codexis, Inc. | TRANSGLUTAMINASE VARIANTS |
| SG11202004185PA (en) | 2017-12-13 | 2020-06-29 | Codexis Inc | Carboxyesterase polypeptides for amide coupling |
| CA3085261A1 (en) | 2017-12-13 | 2019-06-20 | Glaxosmithkline Intellectual Property Development Limited | Carboxyesterase biocatalysts |
| EP3723488B1 (en) | 2017-12-14 | 2025-07-30 | Bactoclear Holdings Pte Ltd. | Therapeutic bacteriocins |
| EP3764798B1 (en) | 2018-03-14 | 2025-12-17 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
| US11820791B2 (en) | 2018-03-14 | 2023-11-21 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
| CA3102968A1 (en) | 2018-06-12 | 2019-12-19 | Codexis, Inc. | Engineered tyrosine ammonia lyase |
| MX2021000327A (es) | 2018-07-09 | 2021-03-25 | Codexis Inc | Enzimas de variante de fosforilasa de nucleosido de purina modificadas geneticamente. |
| MX2021000328A (es) | 2018-07-09 | 2021-03-25 | Codexis Inc | Enzimas de variantes de galactosa oxidasa modificadas geneticamente. |
| KR20210031929A (ko) | 2018-07-09 | 2021-03-23 | 코덱시스, 인코포레이티드 | 조작된 판토테네이트 키나제 변이체 효소 |
| SG11202012107RA (en) | 2018-07-09 | 2021-01-28 | Codexis Inc | Engineered deoxyribose-phosphate aldolases |
| MX2021000322A (es) | 2018-07-09 | 2021-03-25 | Codexis Inc | Enzimas de variante fosfopentomutasa modificada geneticamente. |
| CN112672989A (zh) | 2018-07-12 | 2021-04-16 | 科德克希思公司 | 工程化苯丙氨酸氨裂合酶多肽 |
| CN112805295B (zh) | 2018-07-30 | 2025-10-14 | 泰莱解决方案美国有限责任公司 | 工程化糖基转移酶和甜菊醇糖苷葡糖基化方法 |
| CN113316635A (zh) | 2018-10-29 | 2021-08-27 | 科德克希思公司 | 工程化dna聚合酶变体 |
| AU2019397401A1 (en) | 2018-12-14 | 2021-06-17 | Codexis, Inc. | Engineered tyrosine ammonia lyase |
| BR112021011750A2 (pt) | 2018-12-20 | 2021-08-31 | Codexis, Inc. | Alfa-galactosidase a recombinante e/ou fragmento de alfa-galactosidase a recombinante, composição, sequência de polinucleotídeo recombinante, vetor de expressão, célula hospedeira, métodos para produzir uma variante de alfa-galactosidase a e para tratar e/ou prevenir os sintomas da doença de fabry, composição farmacêutica, e, uso das composições |
| US12503688B2 (en) | 2019-01-15 | 2025-12-23 | Codexis, Inc. | Engineered transaminase polypeptides |
| CA3136828A1 (en) | 2019-05-01 | 2020-11-05 | Codexis, Inc. | Engineered imine reductases and methods for the reductive amination of ketone and amine compounds |
| CN114096662B (zh) | 2019-05-01 | 2025-04-15 | 科德克希思公司 | 工程化葡萄糖脱氢酶和用于酮和胺化合物的还原胺化的方法 |
| MX2021015613A (es) | 2019-07-02 | 2022-02-03 | Codexis Inc | Enzimas variantes acetato cinasa dise?adas. |
| BR112021026326A2 (pt) | 2019-07-02 | 2022-02-08 | Codexis Inc | Sacarose fosforilase engenheirada, composição, sequência de polinucleotídeo, vetor de expressão, célula hospedeira, e, método para produzir uma sacarose fosforilase engenheirada |
| EP4501403A3 (en) | 2019-08-30 | 2025-05-14 | Société des Produits Nestlé S.A. | Engineered lipase variants |
| CA3149870A1 (en) | 2019-09-12 | 2021-03-18 | Codexis, Inc. | Peroxidase activity towards 10-acetyl-3,7-dihydroxyphenoxazine |
| CN114746745B (zh) | 2019-10-02 | 2025-06-17 | 美国雅培糖尿病护理公司 | 通过蛋白质开关检测分析物 |
| CN115175997B (zh) | 2019-11-26 | 2025-12-09 | 科德克希思公司 | 用于化学化合物的羟基化的生物催化剂和方法 |
| AU2020408066A1 (en) | 2019-12-20 | 2022-06-30 | Codexis, Inc. | Engineered acid alpha-glucosidase variants |
| WO2021142393A1 (en) | 2020-01-10 | 2021-07-15 | The Regents Of The University Of California | Biosynthetic platform for the production of olivetolic avid and analogues of olivetolic acid |
| KR20220137057A (ko) | 2020-02-04 | 2022-10-11 | 코덱시스, 인코포레이티드 | 조작된 류신 데카르복실라제 |
| JP2023521772A (ja) | 2020-04-10 | 2023-05-25 | コデクシス, インコーポレイテッド | 操作されたトランスアミナーゼポリペプチド |
| IL296445A (en) | 2020-04-27 | 2022-11-01 | 4D Molecular Therapeutics Inc | Adeno-linked variants, formulations and methods of pulmonary administration |
| BR112022027035A2 (pt) | 2020-07-14 | 2023-04-11 | Pioneer Hi Bred Int | Proteínas inseticidas e métodos para uso das mesmas |
| CA3191900A1 (en) | 2020-08-28 | 2022-03-03 | Codexis, Inc. | Engineered amylase variants |
| WO2022047262A1 (en) | 2020-08-28 | 2022-03-03 | Codexis, Inc. | Engineered protease variants |
| CA3201757A1 (en) | 2020-12-11 | 2022-06-16 | Mathias Schuetz | Recombinant acyl activating enzyme (aae) genes for enhanced biosynthesis of cannabinoids and cannabinoid precursors |
| CA3204825A1 (en) | 2020-12-18 | 2022-06-23 | Codexis, Inc. | Engineered uridine phosphorylase variant enzymes |
| WO2022212832A1 (en) | 2021-04-02 | 2022-10-06 | Codexis, Inc. | Engineered cyclic gmp-amp synthase (cgas) variant enzymes |
| IL305919A (en) | 2021-04-02 | 2023-11-01 | Codexis Inc | Engineered adenylate kinase variant enzymes |
| CN117120599A (zh) | 2021-04-02 | 2023-11-24 | 科德克希思公司 | 工程化鸟苷酸激酶变体酶 |
| CA3215105A1 (en) | 2021-04-02 | 2022-10-06 | Codexis, Inc. | Engineered acetate kinase variant enzymes |
| EP4341395A4 (en) | 2021-05-21 | 2025-07-23 | Syntis Bio Inc | MODIFIED METHIONINE GAMMA LYASE VARIANTS |
| CA3227215A1 (en) | 2021-07-30 | 2023-02-02 | Trish Choudhary | Recombinant prenyltransferase polypeptides engineered for enhanced biosynthesis of cannabinoids |
| CA3227236A1 (en) | 2021-08-19 | 2023-02-23 | Trish Choudhary | Recombinant olivetolic acid cyclase polypeptides engineered for enhanced biosynthesis of cannabinoids |
| CN118103501A (zh) | 2021-10-15 | 2024-05-28 | 科德克希思公司 | 工程化dna聚合酶变体 |
| WO2023069921A1 (en) | 2021-10-19 | 2023-04-27 | Epimeron Usa, Inc. | Recombinant thca synthase polypeptides engineered for enhanced biosynthesis of cannabinoids |
| US12502421B2 (en) | 2021-11-01 | 2025-12-23 | Syntis Bio, Inc. | Engineered leucine decarboxylases |
| WO2023173084A1 (en) | 2022-03-11 | 2023-09-14 | University Of Rochester | Cyclopeptibodies and uses thereof |
| WO2023185184A1 (zh) | 2022-03-31 | 2023-10-05 | 青岛清原化合物有限公司 | 一种单胺氧化酶及其应用 |
| WO2023245171A1 (en) | 2022-06-17 | 2023-12-21 | Synlogic Operating Company, Inc. | Bacteria engineered to treat diseases associated with bile acid metabolism and methods of use thereof |
| WO2023245168A1 (en) | 2022-06-17 | 2023-12-21 | Synlogic Operating Company, Inc. | Bacteria engineered to treat diseases associated with bile acid metabolism and methods of use thereof |
| WO2023250478A1 (en) | 2022-06-23 | 2023-12-28 | Synlogic Operating Company, Inc. | Recombinant bacteria engineered to treat diseases associated with methionine metabolism and methods of use thereof |
| WO2024040020A1 (en) | 2022-08-15 | 2024-02-22 | Absci Corporation | Quantitative affinity activity specific cell enrichment |
| IL322082A (en) | 2023-01-12 | 2025-09-01 | Novartis Ag | Engineered ketoreductase polypeptides |
| WO2025061911A1 (en) | 2023-09-22 | 2025-03-27 | Glaxosmithkline Intellectual Property Development Limited | Methods of producing nucleotide triphosphates (ntps) |
| WO2025144700A1 (en) | 2023-12-27 | 2025-07-03 | Absci Corporation | Nanobody library screening using bacterial surface display |
| US20250297291A1 (en) | 2024-03-19 | 2025-09-25 | General Biological Corporation | Bio-based production of succinic acid using vibrio natriegens |
| WO2025235589A1 (en) | 2024-05-08 | 2025-11-13 | Codexis, Inc. | Dna polymerase variants |
| WO2025240660A1 (en) | 2024-05-15 | 2025-11-20 | Foresite Labs, Llc | Drug development and drug activity determination for coronary artery disease (cad) |
| WO2025242702A1 (en) | 2024-05-23 | 2025-11-27 | Glaxosmithkline Intellectual Property Development Limited | Enzymatic process for producing n-acetyl galactosamine clusters |
Family Cites Families (200)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL154598B (nl) | 1970-11-10 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking. |
| US3817837A (en) | 1971-05-14 | 1974-06-18 | Syva Corp | Enzyme amplification assay |
| US3939350A (en) | 1974-04-29 | 1976-02-17 | Board Of Trustees Of The Leland Stanford Junior University | Fluorescent immunoassay employing total reflection for activation |
| US3996345A (en) | 1974-08-12 | 1976-12-07 | Syva Company | Fluorescence quenching with immunological pairs in immunoassays |
| US3996945A (en) * | 1975-04-07 | 1976-12-14 | Mcdowell James A | Extinguisher for cigarettes or cigars |
| US4277437A (en) | 1978-04-05 | 1981-07-07 | Syva Company | Kit for carrying out chemically induced fluorescence immunoassay |
| US4275149A (en) | 1978-11-24 | 1981-06-23 | Syva Company | Macromolecular environment control in specific receptor assays |
| US4351901A (en) * | 1980-03-24 | 1982-09-28 | Cetus Corporation | Method for single nucleotide alteration |
| US4366241A (en) | 1980-08-07 | 1982-12-28 | Syva Company | Concentrating zone method in heterogeneous immunoassays |
| US4695543A (en) * | 1982-03-23 | 1987-09-22 | Bristol-Myers Company | Alpha Interferon GX-1 |
| US5618697A (en) | 1982-12-10 | 1997-04-08 | Novo Nordisk A/S | Process for preparing a desired protein |
| US4994379A (en) | 1983-03-09 | 1991-02-19 | Cetus Corporation | Modified signal peptides |
| US4816567A (en) | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
| US5538868A (en) | 1984-02-08 | 1996-07-23 | Cetus Oncology Corporation | Recombinant ricin toxin fragments |
| US5422266A (en) * | 1984-12-31 | 1995-06-06 | University Of Georgia Research Foundation, Inc. | Recombinant DNA vectors capable of expressing apoaequorin |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US5176995A (en) | 1985-03-28 | 1993-01-05 | Hoffmann-La Roche Inc. | Detection of viruses by amplification and hybridization |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| EP0316018A3 (en) * | 1985-03-29 | 1989-07-26 | Cetus Oncology Corporation | Modification of dna sequences |
| US6492107B1 (en) | 1986-11-20 | 2002-12-10 | Stuart Kauffman | Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique |
| DE3590766C2 (enExample) * | 1985-03-30 | 1991-01-10 | Marc Genf/Geneve Ch Ballivet | |
| US4959312A (en) | 1985-05-31 | 1990-09-25 | The University Of Tennessee Research Corporation | Full spectrum mutagenesis |
| US4766106A (en) | 1985-06-26 | 1988-08-23 | Cetus Corporation | Solubilization of proteins for pharmaceutical compositions using polymer conjugation |
| EP0208491B1 (en) | 1985-07-03 | 1993-08-25 | Genencor International, Inc. | Hybrid polypeptides and process for their preparation |
| US5866363A (en) | 1985-08-28 | 1999-02-02 | Pieczenik; George | Method and means for sorting and identifying biological information |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| FI72765C (fi) | 1986-02-13 | 1987-07-10 | Valmet Oy | Utbredningsvals eller motsvarande foer vaevnaden i en pappersmaskin samt foerfarande foer framstaellning av denna. |
| DK311186D0 (da) | 1986-06-30 | 1986-06-30 | Novo Industri As | Enzymer |
| US5824469A (en) | 1986-07-17 | 1998-10-20 | University Of Washington | Method for producing novel DNA sequences with biological activity |
| US5418149A (en) * | 1990-07-24 | 1995-05-23 | Hoffmann-La Roche Inc. | Reduction of non-specific amplification glycosylase using DUTP and DNA uracil |
| US4795699A (en) | 1987-01-14 | 1989-01-03 | President And Fellows Of Harvard College | T7 DNA polymerase |
| US4994368A (en) | 1987-07-23 | 1991-02-19 | Syntex (U.S.A.) Inc. | Amplification method for polynucleotide assays |
| DK6488D0 (da) | 1988-01-07 | 1988-01-07 | Novo Industri As | Enzymer |
| WO1989006694A1 (en) * | 1988-01-15 | 1989-07-27 | Trustees Of The University Of Pennsylvania | Process for selection of proteinaceous substances which mimic growth-inducing molecules |
| US5206152A (en) * | 1988-04-08 | 1993-04-27 | Arch Development Corporation | Cloning and expression of early growth regulatory protein genes |
| US4994366A (en) * | 1988-04-28 | 1991-02-19 | Jac Creative Foods, Inc. | Shrimp analog forming process |
| AU3869289A (en) * | 1988-07-14 | 1990-02-05 | Baylor College Of Medicine | Solid phase assembly and reconstruction of biopolymers |
| US5223409A (en) | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
| FR2641793B1 (fr) | 1988-12-26 | 1993-10-01 | Setratech | Procede de recombinaison in vivo de sequences d'adn presentant des mesappariements de bases |
| US4902502A (en) | 1989-01-23 | 1990-02-20 | Cetus Corporation | Preparation of a polymer/interleukin-2 conjugate |
| DD282028A5 (de) | 1989-02-16 | 1990-08-29 | Akad Wissenschaften Ddr | Verfahren zur herstellung einer thermostabilen, hybriden bacillus-beta-1,3-1,4-glukanase |
| EP0385410B1 (en) * | 1989-02-28 | 1996-10-02 | Canon Kabushiki Kaisha | Partially double-stranded oligonucleotide and method for forming oligonucleotide |
| DE3909710A1 (de) * | 1989-03-23 | 1990-09-27 | Boehringer Mannheim Gmbh | Verfahren zur expression eines rekombinanten gens |
| US5106727A (en) | 1989-04-27 | 1992-04-21 | Life Technologies, Inc. | Amplification of nucleic acid sequences using oligonucleotides of random sequences as primers |
| US5043272A (en) | 1989-04-27 | 1991-08-27 | Life Technologies, Incorporated | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers |
| EP0397463A3 (en) | 1989-05-10 | 1991-03-06 | Eastman Kodak Company | Construction of synthetic double-stranded dna sequences |
| US5556750A (en) | 1989-05-12 | 1996-09-17 | Duke University | Methods and kits for fractionating a population of DNA molecules based on the presence or absence of a base-pair mismatch utilizing mismatch repair systems |
| CA2016842A1 (en) | 1989-05-16 | 1990-11-16 | Richard A. Lerner | Method for tapping the immunological repertoire |
| CA2016841C (en) | 1989-05-16 | 1999-09-21 | William D. Huse | A method for producing polymers having a preselected activity |
| US6291161B1 (en) | 1989-05-16 | 2001-09-18 | Scripps Research Institute | Method for tapping the immunological repertiore |
| US6291158B1 (en) | 1989-05-16 | 2001-09-18 | Scripps Research Institute | Method for tapping the immunological repertoire |
| DK169306B1 (da) | 1989-06-14 | 1994-10-10 | Af 18 Juni 1990 As | Middel til fjernelse eller inaktivering af uønskede komponenter i blod eller andre ekstracellulære legemsvæsker samt fremgangsmåde til fremstilling af dextran-O-carbonyl-benzo-18-crown-6 og af dextran-hydroxyethylbenzo-18-crown-6 |
| ATE125300T1 (de) | 1989-06-26 | 1995-08-15 | Lilly Co Eli | Kontrolle der anhäufung von abweichenden expressionsvektoren während der fermentationsverfahren. |
| US5240845A (en) * | 1989-07-11 | 1993-08-31 | Otsuka Pharmaceutical Factory, Ltd. | Mutated streptokinase proteins |
| US5045450A (en) * | 1989-07-13 | 1991-09-03 | Massachusetts Institute Of Technology | Determination of a mutational spectrum |
| IT1231157B (it) | 1989-07-20 | 1991-11-19 | Crc Ricerca Chim | Nuovi geni ibridi funzionali di bacillus thuringiensis ottenuti mediante ricombinazione in vivo. |
| US5514787A (en) * | 1989-07-21 | 1996-05-07 | Washington University | DNA sequences encoding human membrane cofactor protein (MCP) |
| US5574205A (en) | 1989-07-25 | 1996-11-12 | Cell Genesys | Homologous recombination for universal donor cells and chimeric mammalian hosts |
| US5023171A (en) | 1989-08-10 | 1991-06-11 | Mayo Foundation For Medical Education And Research | Method for gene splicing by overlap extension using the polymerase chain reaction |
| US5360726A (en) | 1989-09-12 | 1994-11-01 | Board Of Trustees Operating Michigan State University | Polypeptides enabling sorting of proteins to vacuoles in plants |
| US5030568A (en) * | 1989-09-29 | 1991-07-09 | Minnesota Mining And Manufacturing Company | Bioconversion of naphthalene monomers |
| WO1991006570A1 (en) | 1989-10-25 | 1991-05-16 | The University Of Melbourne | HYBRID Fc RECEPTOR MOLECULES |
| WO1991007506A1 (en) | 1989-11-08 | 1991-05-30 | The United States Of America, Represented By The Secretary, United States Department Of Commerce | A method of synthesizing double-stranded dna molecules |
| DE4000939A1 (de) * | 1990-01-15 | 1991-07-18 | Brem Gottfried Prof Dr Dr | Verfahren zur antikoerpergewinnung |
| US5427930A (en) | 1990-01-26 | 1995-06-27 | Abbott Laboratories | Amplification of target nucleic acids using gap filling ligase chain reaction |
| EP0439182B1 (en) | 1990-01-26 | 1996-04-24 | Abbott Laboratories | Improved method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions |
| EP0527809B1 (en) | 1990-04-05 | 1995-08-16 | CREA, Roberto | Walk-through mutagenesis |
| AU7791991A (en) | 1990-04-24 | 1991-11-11 | Stratagene | Methods for phenotype creation from multiple gene populations |
| US5427908A (en) * | 1990-05-01 | 1995-06-27 | Affymax Technologies N.V. | Recombinant library screening methods |
| US5877402A (en) | 1990-05-01 | 1999-03-02 | Rutgers, The State University Of New Jersey | DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein |
| US5851813A (en) | 1990-07-12 | 1998-12-22 | President And Fellows Of Harvard College | Primate lentivirus antigenic compositions |
| US5169764A (en) | 1990-08-08 | 1992-12-08 | Regeneron Pharmaceuticals, Inc. | Multitrophic and multifunctional chimeric neurotrophic factors, and nucleic acids and plasmids encoding the chimeras |
| CA2089497A1 (en) * | 1990-08-15 | 1992-02-16 | Lendon Payne | Self-assembling replication defective hybrid virus particles |
| US5264563A (en) | 1990-08-24 | 1993-11-23 | Ixsys Inc. | Process for synthesizing oligonucleotides with random codons |
| JP3306063B2 (ja) | 1990-08-24 | 2002-07-24 | イグジス, インコーポレイテッド | ランダムコドンを有するオリゴヌクレオチドを合成する方法 |
| CA2049879A1 (en) | 1990-08-30 | 1992-03-01 | Keith C. Backman | Controlled initial target-dependent production of templates for ligase chain reaction |
| US5871974A (en) | 1990-09-28 | 1999-02-16 | Ixsys Inc. | Surface expression libraries of heteromeric receptors |
| IL99553A0 (en) | 1990-09-28 | 1992-08-18 | Ixsys Inc | Compositions containing oligonucleotides linked to expression elements,a kit for the preparation of vectors useful for the expression of a diverse population of random peptides and methods utilizing the same |
| US5698426A (en) | 1990-09-28 | 1997-12-16 | Ixsys, Incorporated | Surface expression libraries of heteromeric receptors |
| US5770434A (en) | 1990-09-28 | 1998-06-23 | Ixsys Incorporated | Soluble peptides having constrained, secondary conformation in solution and method of making same |
| US5858725A (en) | 1990-10-10 | 1999-01-12 | Glaxo Wellcome Inc. | Preparation of chimaeric antibodies using the recombinant PCR strategy |
| US5187083A (en) | 1990-11-13 | 1993-02-16 | Specialty Laboratories, Inc. | Rapid purification of DNA |
| US5234824A (en) | 1990-11-13 | 1993-08-10 | Specialty Laboratories, Inc. | Rapid purification of DNA |
| US5489523A (en) | 1990-12-03 | 1996-02-06 | Stratagene | Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I |
| WO1992009690A2 (en) | 1990-12-03 | 1992-06-11 | Genentech, Inc. | Enrichment method for variant proteins with altered binding properties |
| IL100460A (en) | 1990-12-20 | 1997-06-10 | Ixsys | Method for optimization of binding proteins and nucleic acids encoding a binding protein produced thereby |
| US5795747A (en) | 1991-04-16 | 1998-08-18 | Evotec Biosystems Gmbh | Process for manufacturing new biopolymers |
| DE4112440C1 (enExample) | 1991-04-16 | 1992-10-22 | Diagen Institut Fuer Molekularbiologische Diagnostik Gmbh, 4000 Duesseldorf, De | |
| US5512463A (en) | 1991-04-26 | 1996-04-30 | Eli Lilly And Company | Enzymatic inverse polymerase chain reaction library mutagenesis |
| US5514568A (en) | 1991-04-26 | 1996-05-07 | Eli Lilly And Company | Enzymatic inverse polymerase chain reaction |
| DE69213112T2 (de) | 1991-06-20 | 1997-04-03 | Hoffmann La Roche | Verbesserte Methoden zur Nukleinsäure-Amplifizierung |
| WO1993000103A1 (en) | 1991-06-21 | 1993-01-07 | The Wistar Institute Of Anatomy And Biology | Chimeric envelope proteins for viral targeting |
| AU2317192A (en) * | 1991-07-01 | 1993-02-11 | Berlex Laboratories, Inc. | Novel mutagenesis methods and compositions |
| US6071889A (en) | 1991-07-08 | 2000-06-06 | Neurospheres Holdings Ltd. | In vivo genetic modification of growth factor-responsive neural precursor cells |
| US5223408A (en) | 1991-07-11 | 1993-06-29 | Genentech, Inc. | Method for making variant secreted proteins with altered properties |
| GB9115364D0 (en) | 1991-07-16 | 1991-08-28 | Wellcome Found | Antibody |
| US5279952A (en) | 1991-08-09 | 1994-01-18 | Board Of Regents, The University Of Texas System | PCR-based strategy of constructing chimeric DNA molecules |
| JP2001524926A (ja) | 1991-09-18 | 2001-12-04 | アフィマックス テクノロジーズ ナームロゼ フェンノートシャップ | オリゴマーの雑多ライブラリーの集合体を合成する方法 |
| US5565332A (en) * | 1991-09-23 | 1996-10-15 | Medical Research Council | Production of chimeric antibodies - a combinatorial approach |
| AU665025B2 (en) | 1991-09-23 | 1995-12-14 | Cambridge Antibody Technology Limited | Production of chimeric antibodies - a combinatorial approach |
| US5872215A (en) * | 1991-12-02 | 1999-02-16 | Medical Research Council | Specific binding members, materials and methods |
| GB9125979D0 (en) | 1991-12-06 | 1992-02-05 | Wellcome Found | Antibody |
| EP0625205A1 (en) | 1992-01-30 | 1994-11-23 | Genzyme Limited | Chiral synthesis with modified enzymes |
| US5773267A (en) * | 1992-02-07 | 1998-06-30 | Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University | D29 shuttle phasmids and uses thereof |
| GB9202796D0 (en) | 1992-02-11 | 1992-03-25 | Wellcome Found | Antiviral antibody |
| US5843643A (en) | 1992-02-21 | 1998-12-01 | Ratner; Paul L. | Site-specific transfection of eukaryotic cells using polypeptide-linked recombinant nucleic acid |
| WO1993018141A1 (en) | 1992-03-02 | 1993-09-16 | Biogen, Inc. | Thrombin receptor antagonists |
| WO1993018175A1 (en) * | 1992-03-10 | 1993-09-16 | Life Technologies, Inc. | Udg-facilitated mutagenesis |
| AU687347B2 (en) | 1992-03-16 | 1998-02-26 | Jacob Nathaniel Wohlstadter | Selection methods |
| DE69333823T2 (de) | 1992-03-24 | 2006-05-04 | Cambridge Antibody Technology Ltd., Melbourn | Verfahren zur herstellung von gliedern von spezifischen bindungspaaren |
| EP0633944B1 (en) | 1992-03-31 | 2000-11-08 | Abbott Laboratories | Method of multiplex ligase chain reaction |
| US5316935A (en) | 1992-04-06 | 1994-05-31 | California Institute Of Technology | Subtilisin variants suitable for hydrolysis and synthesis in organic media |
| US5474896A (en) * | 1992-05-05 | 1995-12-12 | Institut Pasteur | Nucleotide sequence encoding the enzyme I-SceI and the uses thereof |
| WO1993024656A1 (en) | 1992-05-29 | 1993-12-09 | Abbott Laboratories | Ligase chain reaction starting with rna sequences |
| WO1993025237A1 (en) | 1992-06-15 | 1993-12-23 | City Of Hope | Chimeric anti-cea antibody |
| JP2979178B2 (ja) | 1992-07-10 | 1999-11-15 | エナジー バイオシステムズ コーポレーション | 脱硫用生物触媒をコードする組換えdna |
| US6107062A (en) | 1992-07-30 | 2000-08-22 | Inpax, Inc. | Antisense viruses and antisense-ribozyme viruses |
| AU4848393A (en) | 1992-09-25 | 1994-04-26 | Abbott Laboratories | Ligase chain reaction method for detecting small mutations |
| WO1994009817A1 (en) | 1992-11-04 | 1994-05-11 | City Of Hope | Novel antibody construct |
| CA2148838A1 (en) | 1992-11-10 | 1994-05-26 | William D. Huse | Soluble peptides having constrained, secondary conformation in solution and method of making same |
| DE4239311C2 (de) | 1992-11-23 | 1996-04-18 | Guehring Joerg Dr | Bohrer, insbesondere Spitzbohrwerkzeug mit austauschbarem Schneideinsatz |
| WO1994012632A1 (en) * | 1992-11-27 | 1994-06-09 | University College London | Improvements in nucleic acid synthesis by pcr |
| US5360728A (en) | 1992-12-01 | 1994-11-01 | Woods Hole Oceanographic Institution (W.H.O.I.) | Modified apoaequorin having increased bioluminescent activity |
| ATE199392T1 (de) | 1992-12-04 | 2001-03-15 | Medical Res Council | Multivalente und multispezifische bindungsproteine, deren herstellung und verwendung |
| US5541311A (en) | 1992-12-07 | 1996-07-30 | Third Wave Technologies, Inc. | Nucleic acid encoding synthesis-deficient thermostable DNA polymerase |
| JP2879303B2 (ja) * | 1993-01-14 | 1999-04-05 | 佑 本庶 | cDNAライブラリーの作製方法、および新規なポリペプチドとそれをコードするDNA |
| US5571708A (en) | 1993-04-19 | 1996-11-05 | Bristol-Myers Squibb Company | Thrombin-activatable plasminogen activator |
| US5501962A (en) | 1993-06-21 | 1996-03-26 | G. D. Searle & Co. | Interleuken-3 (IL-3) human/murine hybrid polypeptides and recombinant production of the same |
| IT1264712B1 (it) | 1993-07-13 | 1996-10-04 | Eniricerche Spa | Metodo di sintesi biologica di peptidi |
| US5547859A (en) | 1993-08-02 | 1996-08-20 | Goodman; Myron F. | Chain-terminating nucleotides for DNA sequencing methods |
| US5498531A (en) | 1993-09-10 | 1996-03-12 | President And Fellows Of Harvard College | Intron-mediated recombinant techniques and reagents |
| US6015686A (en) | 1993-09-15 | 2000-01-18 | Chiron Viagene, Inc. | Eukaryotic layered vector initiation systems |
| IL107268A0 (en) | 1993-10-12 | 1994-01-25 | Yeda Res & Dev | Molecules influencing the shedding of the tnf receptor, their preparation and their use |
| AU8126694A (en) | 1993-10-26 | 1995-05-22 | Affymax Technologies N.V. | Arrays of nucleic acid probes on biological chips |
| US6027877A (en) | 1993-11-04 | 2000-02-22 | Gene Check, Inc. | Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, purification of amplified DNA samples and allele identification |
| US5556772A (en) | 1993-12-08 | 1996-09-17 | Stratagene | Polymerase compositions and uses thereof |
| DE4343591A1 (de) | 1993-12-21 | 1995-06-22 | Evotec Biosystems Gmbh | Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US6117679A (en) * | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US6395547B1 (en) * | 1994-02-17 | 2002-05-28 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US6406855B1 (en) * | 1994-02-17 | 2002-06-18 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
| US6309883B1 (en) | 1994-02-17 | 2001-10-30 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US6165793A (en) | 1996-03-25 | 2000-12-26 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US5928905A (en) | 1995-04-18 | 1999-07-27 | Glaxo Group Limited | End-complementary polymerase reaction |
| US6335160B1 (en) | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
| US5834252A (en) | 1995-04-18 | 1998-11-10 | Glaxo Group Limited | End-complementary polymerase reaction |
| US5837458A (en) | 1994-02-17 | 1998-11-17 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US5521077A (en) | 1994-04-28 | 1996-05-28 | The Leland Stanford Junior University | Method of generating multiple protein variants and populations of protein variants prepared thereby |
| US5728821A (en) * | 1994-08-04 | 1998-03-17 | Bristol-Myers Squibb Company | Mutant BR96 antibodies reactive with human carcinomas |
| WO1996017056A1 (en) * | 1994-12-02 | 1996-06-06 | Institut Pasteur | Hypermutagenesis |
| US5514588A (en) | 1994-12-13 | 1996-05-07 | Exxon Research And Engineering Company | Surfactant-nutrients for bioremediation of hydrocarbon contaminated soils and water |
| US5629179A (en) | 1995-03-17 | 1997-05-13 | Novagen, Inc. | Method and kit for making CDNA library |
| CA2219136A1 (en) | 1995-04-24 | 1996-10-31 | Chromaxome Corp. | Methods for generating and screening novel metabolic pathways |
| CA2222744C (en) | 1995-05-31 | 2008-03-25 | Amersham Life Science, Inc. | Thermostable dna polymerases |
| CA2151183C (en) * | 1995-06-07 | 2005-05-17 | Slawomir Jonasz | Composite compression molded article, composition therefor and process for manufacture thereof, and use |
| US5667974A (en) | 1995-06-07 | 1997-09-16 | Abbott Laboratories | Method for detecting nucleic acid sequences using competitive amplification |
| US6030779A (en) | 1995-07-18 | 2000-02-29 | Diversa Corporation | Screening for novel bioactivities |
| US6057103A (en) | 1995-07-18 | 2000-05-02 | Diversa Corporation | Screening for novel bioactivities |
| US5958672A (en) | 1995-07-18 | 1999-09-28 | Diversa Corporation | Protein activity screening of clones having DNA from uncultivated microorganisms |
| US6004788A (en) | 1995-07-18 | 1999-12-21 | Diversa Corporation | Enzyme kits and libraries |
| US6168919B1 (en) | 1996-07-17 | 2001-01-02 | Diversa Corporation | Screening methods for enzymes and enzyme kits |
| DE69620766T2 (de) | 1995-08-11 | 2004-11-18 | Novozymes A/S | Verfahren zur herstellung von polypeptidabkömmlingen |
| US5962258A (en) | 1995-08-23 | 1999-10-05 | Diversa Corporation | Carboxymethyl cellulase fromthermotoga maritima |
| US6051409A (en) | 1995-09-25 | 2000-04-18 | Novartis Finance Corporation | Method for achieving integration of exogenous DNA delivered by non-biological means to plant cells |
| US5756316A (en) | 1995-11-02 | 1998-05-26 | Genencor International, Inc. | Molecular cloning by multimerization of plasmids |
| US5939250A (en) | 1995-12-07 | 1999-08-17 | Diversa Corporation | Production of enzymes having desired activities by mutagenesis |
| EP1130090A3 (en) | 1995-12-07 | 2003-11-26 | Diversa Corporation | Method of screening for thermostable enzyme activity |
| US6171820B1 (en) | 1995-12-07 | 2001-01-09 | Diversa Corporation | Saturation mutagenesis in directed evolution |
| US6358709B1 (en) | 1995-12-07 | 2002-03-19 | Diversa Corporation | End selection in directed evolution |
| US5965408A (en) | 1996-07-09 | 1999-10-12 | Diversa Corporation | Method of DNA reassembly by interrupting synthesis |
| US5830696A (en) | 1996-12-05 | 1998-11-03 | Diversa Corporation | Directed evolution of thermophilic enzymes |
| US20030215798A1 (en) | 1997-06-16 | 2003-11-20 | Diversa Corporation | High throughput fluorescence-based screening for novel enzymes |
| US6352842B1 (en) | 1995-12-07 | 2002-03-05 | Diversa Corporation | Exonucease-mediated gene assembly in directed evolution |
| US6238884B1 (en) | 1995-12-07 | 2001-05-29 | Diversa Corporation | End selection in directed evolution |
| US6361974B1 (en) | 1995-12-07 | 2002-03-26 | Diversa Corporation | Exonuclease-mediated nucleic acid reassembly in directed evolution |
| DE69733059T2 (de) | 1996-01-10 | 2006-03-02 | Novozymes A/S | Methode zur invivo produktion einer mutanten-bibliothek in zellen |
| US5871969A (en) * | 1996-02-12 | 1999-02-16 | Human Genome Sciences, Inc. | Nucleic acids encoding human neuronal attachment factor-1 |
| US6096548A (en) | 1996-03-25 | 2000-08-01 | Maxygen, Inc. | Method for directing evolution of a virus |
| US5783431A (en) | 1996-04-24 | 1998-07-21 | Chromaxome Corporation | Methods for generating and screening novel metabolic pathways |
| EP0906420A1 (en) * | 1996-06-07 | 1999-04-07 | Massachusetts Institute Of Technology | Programmed sequential mutagenesis |
| US5763239A (en) | 1996-06-18 | 1998-06-09 | Diversa Corporation | Production and use of normalized DNA libraries |
| US6093873A (en) | 1996-08-19 | 2000-07-25 | Institut National De La Sante Et De La Recherche Medicale | Genetically engineered mice containing alterations in the gene encoding RXR |
| JP2001504325A (ja) | 1996-09-27 | 2001-04-03 | マキシジェン,インコーポレイテッド | 回帰的配列シャフリングおよび選択による遺伝子治療の最適化のための方法 |
| AU4597197A (en) | 1996-09-27 | 1998-04-17 | Introgene B.V. | Methods for optimization of gene therapy by recursive sequence shuffling and selection |
| CN1208456C (zh) | 1996-12-20 | 2005-06-29 | 诺维信公司 | 体内重组 |
| US6326204B1 (en) * | 1997-01-17 | 2001-12-04 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
| IL131986A0 (en) | 1997-03-18 | 2001-03-19 | Novo Nordisk As | Shuffling of heterologous dna sequences |
| US6159687A (en) | 1997-03-18 | 2000-12-12 | Novo Nordisk A/S | Methods for generating recombined polynucleotides |
| NZ337900A (en) | 1997-03-18 | 2001-05-25 | Novo Nordisk As | DNA shuffling for randomised heterologous and homogenous 3' and 5' primers for construction of DNA libraries for generation of more desirable mutants |
| ATE418608T1 (de) | 1997-03-18 | 2009-01-15 | Novozymes As | Methode zur herstellung einer bibliothek mittels dna-shuffling |
| US5948653A (en) | 1997-03-21 | 1999-09-07 | Pati; Sushma | Sequence alterations using homologous recombination |
| US6153410A (en) | 1997-03-25 | 2000-11-28 | California Institute Of Technology | Recombination of polynucleotide sequences using random or defined primers |
| US6051049A (en) | 1997-05-29 | 2000-04-18 | Exothermic Distribution Corporation | Utilization of strontium aluminate in steelmaking |
| IL136574A0 (en) | 1997-12-08 | 2001-06-14 | California Inst Of Techn | A method for forming a polynucleotide of desired properties |
| US6087341A (en) | 1998-02-12 | 2000-07-11 | The Board Of Trustees Of The Leland Standford Junior University | Introduction of nucleic acid into skin cells by topical application |
| US6365408B1 (en) | 1998-06-19 | 2002-04-02 | Maxygen, Inc. | Methods of evolving a polynucleotides by mutagenesis and recombination |
| US6376246B1 (en) * | 1999-02-05 | 2002-04-23 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
| US6436675B1 (en) * | 1999-09-28 | 2002-08-20 | Maxygen, Inc. | Use of codon-varied oligonucleotide synthesis for synthetic shuffling |
| EP1152058A1 (en) * | 2000-05-03 | 2001-11-07 | Institut Curie | Methods and compositions for effecting homologous recombination |
| US6355160B1 (en) | 2000-07-21 | 2002-03-12 | Cecil A. Wiseman | Gray-water recycling system |
-
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- 1996-12-18 US US08/769,062 patent/US6335160B1/en not_active Expired - Lifetime
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