JP4426094B2 - αアミラーゼ変異体 - Google Patents
αアミラーゼ変異体 Download PDFInfo
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- JP4426094B2 JP4426094B2 JP2000519071A JP2000519071A JP4426094B2 JP 4426094 B2 JP4426094 B2 JP 4426094B2 JP 2000519071 A JP2000519071 A JP 2000519071A JP 2000519071 A JP2000519071 A JP 2000519071A JP 4426094 B2 JP4426094 B2 JP 4426094B2
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- amylase
- seq
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- amino acid
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Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38609—Protease or amylase in solid compositions only
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01001—Alpha-amylase (3.2.1.1)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
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- Microbiology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Enzymes And Modification Thereof (AREA)
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- Detergent Compositions (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK1240/97 | 1997-10-30 | ||
| DK124097 | 1997-10-30 | ||
| DK199800936 | 1998-07-14 | ||
| DKPA199800936 | 1998-07-14 | ||
| PCT/DK1998/000471 WO1999023211A1 (en) | 1997-10-30 | 1998-10-30 | α-AMYLASE MUTANTS |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2001521739A JP2001521739A (ja) | 2001-11-13 |
| JP2001521739A5 JP2001521739A5 (enExample) | 2006-01-05 |
| JP4426094B2 true JP4426094B2 (ja) | 2010-03-03 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000519071A Expired - Lifetime JP4426094B2 (ja) | 1997-10-30 | 1998-10-30 | αアミラーゼ変異体 |
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| Country | Link |
|---|---|
| US (8) | US6204232B1 (enExample) |
| EP (4) | EP2388267A1 (enExample) |
| JP (1) | JP4426094B2 (enExample) |
| CN (2) | CN100593034C (enExample) |
| AT (1) | ATE490311T1 (enExample) |
| AU (1) | AU9737398A (enExample) |
| BR (2) | BRPI9816290B1 (enExample) |
| CA (2) | CA2308119C (enExample) |
| DE (1) | DE69842027D1 (enExample) |
| ES (1) | ES2515218T3 (enExample) |
| WO (1) | WO1999023211A1 (enExample) |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US6440716B1 (en) * | 1995-02-03 | 2002-08-27 | Novozymes A/S | α-amylase mutants |
| ES2436066T3 (es) * | 1997-10-13 | 2013-12-26 | Novozymes A/S | Mutantes de alfa-amilasa |
| US6361989B1 (en) * | 1997-10-13 | 2002-03-26 | Novozymes A/S | α-amylase and α-amylase variants |
| US6410295B1 (en) | 1999-03-30 | 2002-06-25 | Novozymes A/S | Alpha-amylase variants |
| KR100787392B1 (ko) * | 1999-03-31 | 2007-12-21 | 노보자임스 에이/에스 | 알칼리 α-아밀라제 활성을 가지는 폴리펩티드 및 그것을코드하는 핵산 |
| US6623948B1 (en) * | 1999-03-31 | 2003-09-23 | Novozymes A/S | Nucleic acid sequences encoding alkaline alpha-amylases |
| CN1212395C (zh) | 1999-06-10 | 2005-07-27 | 花王株式会社 | 突变α－淀粉酶 |
| US7005288B1 (en) | 1999-11-10 | 2006-02-28 | Novozymes A/S | Fungamyl-like alpha-amylase variants |
| EP1980614A3 (en) * | 1999-11-10 | 2009-04-08 | Novozymes A/S | Fungamyl-like Alpha-Amylase Variants |
| US6350599B1 (en) | 2000-01-12 | 2002-02-26 | Novozymes A/S | Pullulanase variants and methods for preparing such variants with predetermined properties |
| WO2001064852A1 (en) * | 2000-03-03 | 2001-09-07 | Novozymes A/S | Polypeptides having alkaline alpha-amylase activity and nucleic acids encoding same |
| EP1263942B1 (en) * | 2000-03-08 | 2013-11-06 | Novozymes A/S | Variants with altered properties |
| CN100491525C (zh) | 2000-07-28 | 2009-05-27 | 汉高两合股份公司 | 从芽孢杆菌a7-7(dsm 12368)中提取的新型淀粉分解酶以及含有该新型淀粉分解酶的洗涤剂和清洗剂 |
| DE10036752C2 (de) * | 2000-07-28 | 2003-02-20 | Henkel Kgaa | Wasch- und Reinigungsmittel mit einem neuen amylolytischen Enzym aus Bacillus sp. A 7-7(DSM 12368) |
| US20020155574A1 (en) * | 2000-08-01 | 2002-10-24 | Novozymes A/S | Alpha-amylase mutants with altered properties |
| JP4426716B2 (ja) * | 2000-10-11 | 2010-03-03 | 花王株式会社 | 高生産性α−アミラーゼ |
| EP1337648B1 (de) | 2000-11-28 | 2007-09-19 | Henkel Kommanditgesellschaft auf Aktien | Cyclodextrin -glucanotransferase(cg tase) aus bacillus agaradherens(dsm 9948)sowie wasch-und reinigungsmittel mit dieser neuen cyclodextrin-glucanotransferase |
| ATE510023T1 (de) * | 2001-02-21 | 2011-06-15 | Verenium Corp | Enzyme mit alpha-amylase-aktivität und verfahren zu deren verwendung |
| EP2159279A3 (en) | 2001-05-15 | 2010-05-12 | Novozymes A/S | Alpha-amylase variant with altered properties |
| DE10138753B4 (de) * | 2001-08-07 | 2017-07-20 | Henkel Ag & Co. Kgaa | Wasch- und Reinigungsmittel mit Hybrid-Alpha-Amylasen |
| JP4897186B2 (ja) * | 2002-03-27 | 2012-03-14 | 花王株式会社 | 変異アルカリセルラーゼ |
| US7226771B2 (en) | 2002-04-19 | 2007-06-05 | Diversa Corporation | Phospholipases, nucleic acids encoding them and methods for making and using them |
| BRPI0309391B1 (pt) | 2002-04-19 | 2018-10-30 | Dsm Food Specialties B V | vetor compreendendo ácido nucléico que codifica polipeptídeo com atividade de fosfolipase, célula transformada, preparado de proteína, método de preparação e métodos relacionados |
| EP2298904B1 (en) | 2002-06-14 | 2017-05-24 | BP Corporation North America Inc. | Xylanases, nucleic acids encoding them and methods for making and using them |
| KR20050052666A (ko) * | 2002-10-10 | 2005-06-03 | 다이버사 코포레이션 | 프로테아제, 이를 암호화하는 핵산, 및 이를 제조하고사용하는 방법 |
| DE10362172B4 (de) * | 2003-03-05 | 2009-04-02 | Henkel Ag & Co. Kgaa | Wasch-und Reinigungsmittel mit Alpha-Amylase-Varianten mit verbesserter Alkaliaktivität |
| PL3023498T3 (pl) | 2003-03-06 | 2019-05-31 | Basf Enzymes Llc | Amylazy, kodujące je kwasy nukleinowe i sposoby ich wytwarzania i zastosowania |
| AU2004277368B2 (en) | 2003-03-07 | 2011-01-20 | Dsm Ip Assets B.V. | Hydrolases, nucleic acids encoding them and mehods for making and using them |
| EP1608766A4 (en) * | 2003-03-20 | 2006-11-02 | Diversa Corp | GLUCOSIDASES, THESE CODING NUCLEIC ACIDS AND METHOD FOR THE PRODUCTION AND USE THEREOF |
| WO2004090099A2 (en) | 2003-04-04 | 2004-10-21 | Diversa Corporation | Pectate lyases, nucleic acids encoding them and methods for making and using them |
| EP1654355B1 (en) | 2003-06-13 | 2010-04-21 | Danisco A/S | Variant pseudomonas polypeptides having a non-maltogenic exoamylase activity and their use in preparing food products |
| DK1641910T3 (da) | 2003-07-02 | 2013-05-21 | Verenium Corp | Glucanaser, nucleinsyrer, som koder for disse, og fremgangsmåder til at fremstille og benytte disse |
| US8143048B2 (en) | 2003-07-07 | 2012-03-27 | Danisco A/S | Exo-specific amylase polypeptides, nucleic acids encoding those polypeptides and uses thereof |
| BRPI0412401A (pt) | 2003-07-07 | 2006-09-05 | Genencor Int | polipeptìdeos da amilase exoespecìficos, ácidos nucléicos que codificam tais polipeptìdeos e usos dos mesmos |
| WO2005021714A2 (en) | 2003-08-11 | 2005-03-10 | Diversa Corporation | Laccases, nucleic acids encoding them and methods for making and using them |
| CN107151662B (zh) * | 2004-07-05 | 2021-06-29 | 诺维信公司 | 具有改变特性的α-淀粉酶变异体 |
| NZ552459A (en) * | 2004-07-07 | 2010-05-28 | Danisco | Non-maltogenic exoamylase variants |
| DE102004047777B4 (de) | 2004-10-01 | 2018-05-09 | Basf Se | Alpha-Amylase-Varianten mit erhöhter Lösungsmittelstabilität, Verfahren zu deren Herstellung sowie deren Verwendung |
| KR101393679B1 (ko) | 2005-03-15 | 2014-05-21 | 비피 코포레이션 노쓰 아메리카 인코포레이티드 | 셀룰라제, 이것을 암호화하는 핵산 및 이들을 제조 및사용하는 방법 |
| US8030050B2 (en) | 2005-07-07 | 2011-10-04 | Danisco A/S | Modified amylases from Pseudomonas species |
| DE102006038448A1 (de) | 2005-12-28 | 2008-02-21 | Henkel Kgaa | Enzym-haltiges Reinigungsmittel |
| US20090324574A1 (en) | 2006-02-02 | 2009-12-31 | Verenium Corporation | Esterases and Related Nucleic Acids and Methods |
| AU2006338208B2 (en) | 2006-02-10 | 2013-10-24 | Bp Corporation North America Inc. | Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them |
| EP2548954A1 (en) | 2006-02-14 | 2013-01-23 | Verenium Corporation | Xylanases, nucleic acids encoding them and methods for making and using them |
| BRPI0713286A2 (pt) | 2006-06-19 | 2012-10-09 | Danisco | polipeptìdeo |
| DK2069389T3 (en) | 2006-08-04 | 2015-01-12 | Bp Corp North America Inc | Glucanases, nucleic acids encoding them, and processes for their preparation and use |
| ES2451266T3 (es) | 2006-09-21 | 2014-03-26 | Dsm Ip Assets B.V. | Fosfolipasas, ácidos nucleicos que las codifican, y métodos para obtenerlas y usarlas |
| ES2531167T5 (es) | 2006-12-21 | 2018-05-21 | Basf Enzymes Llc | Amilasas y glucoamilasas, ácidos nucleicos que las codifican y métodos para formarlas y utilizarlas |
| EP2074136A4 (en) | 2007-01-30 | 2012-11-07 | Verenium Corp | ENZYMES FOR TREATING LIGNOCELLULOSE, FOR SUCH CODING NUCLEIC ACIDS, AND METHOD FOR THE PRODUCTION AND USE THEREOF |
| KR20100014954A (ko) * | 2007-03-09 | 2010-02-11 | 다니스코 유에스 인크. | 호알칼리성 바실러스 종 α-아밀라아제 변이체, α-아밀라아제 변이체를 함유하는 조성물 및 사용 방법 |
| DK2171055T3 (en) * | 2007-06-06 | 2016-06-27 | Danisco Us Inc | Process for the improvement of protein many properties |
| MX2010003600A (es) | 2007-10-03 | 2010-12-14 | Verenium Corp | Xilanasas, acidos nucleicos que las codifican, y metodos para hacerlas y usarlas. |
| US8066818B2 (en) | 2008-02-08 | 2011-11-29 | The Procter & Gamble Company | Water-soluble pouch |
| US20090209447A1 (en) * | 2008-02-15 | 2009-08-20 | Michelle Meek | Cleaning compositions |
| EP2100947A1 (en) | 2008-03-14 | 2009-09-16 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
| US20090233830A1 (en) | 2008-03-14 | 2009-09-17 | Penny Sue Dirr | Automatic detergent dishwashing composition |
| US8198062B2 (en) | 2008-08-29 | 2012-06-12 | Dsm Ip Assets B.V. | Hydrolases, nucleic acids encoding them and methods for making and using them |
| US8357503B2 (en) | 2008-08-29 | 2013-01-22 | Bunge Oils, Inc. | Hydrolases, nucleic acids encoding them and methods for making and using them |
| US8153391B2 (en) | 2008-08-29 | 2012-04-10 | Bunge Oils, Inc. | Hydrolases, nucleic acids encoding them and methods for making and using them |
| EP2166092A1 (en) | 2008-09-18 | 2010-03-24 | The Procter and Gamble Company | Detergent composition |
| EP2166076A1 (en) | 2008-09-23 | 2010-03-24 | The Procter & Gamble Company | Cleaning composition |
| EP2166075A1 (en) | 2008-09-23 | 2010-03-24 | The Procter and Gamble Company | Cleaning composition |
| EP2166073A1 (en) * | 2008-09-23 | 2010-03-24 | The Procter & Gamble Company | Cleaning composition |
| US20100125046A1 (en) | 2008-11-20 | 2010-05-20 | Denome Frank William | Cleaning products |
| EP3998328A1 (en) | 2009-02-09 | 2022-05-18 | The Procter & Gamble Company | Detergent composition |
| WO2010097436A1 (en) | 2009-02-27 | 2010-09-02 | Novozymes A/S | Mutant cells having reduced expression of metallopeptidase, suitable for recombinant polypeptide production |
| EP2451915A1 (en) | 2009-07-09 | 2012-05-16 | The Procter & Gamble Company | A catalytic laundry detergent composition comprising relatively low levels of water-soluble electrolyte |
| MX2012000486A (es) | 2009-07-09 | 2012-01-27 | Procter & Gamble | Una composicion detergente catalitica para lavanderia que comprende niveles relativamente bajos de electrolitos solubles en agua. |
| PL2292725T5 (pl) | 2009-08-13 | 2022-11-07 | The Procter And Gamble Company | Sposób prania tkanin w niskiej temperaturze |
| UA111708C2 (uk) | 2009-10-16 | 2016-06-10 | Бандж Ойлз, Інк. | Спосіб рафінування олії |
| UA109884C2 (uk) | 2009-10-16 | 2015-10-26 | Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування | |
| CN102652175B (zh) | 2009-12-09 | 2016-02-10 | 宝洁公司 | 织物和家居护理产品 |
| PL2333042T3 (pl) | 2009-12-10 | 2015-12-31 | Procter & Gamble | Produkt do automatycznych zmywarek do mycia naczyń i jego wykorzystanie |
| EP2333040B2 (en) | 2009-12-10 | 2019-11-13 | The Procter & Gamble Company | Detergent composition |
| ES2423580T5 (es) | 2009-12-10 | 2021-06-17 | Procter & Gamble | Método y uso de una composición para lavado de vajillas |
| ES2422593T3 (es) | 2009-12-10 | 2013-09-12 | Procter & Gamble | Método y uso de una composición para lavavajillas |
| CN102762723A (zh) | 2009-12-21 | 2012-10-31 | 诺维信股份有限公司 | 用于在巯基-二硫键氧还酶缺陷的细菌突变体细胞中产生异源多肽的方法 |
| DK2521774T3 (en) | 2010-01-04 | 2016-09-26 | Novozymes As | ALFA AMYLASE VARANTS AND POLYNUCLEOTIDES CODING THESE |
| EP2357220A1 (en) * | 2010-02-10 | 2011-08-17 | The Procter & Gamble Company | Cleaning composition comprising amylase variants with high stability in the presence of a chelating agent |
| EP3892709A3 (en) * | 2010-02-10 | 2022-01-19 | Novozymes A/S | Variants and compositions comprising variants with high stability in presence of a chelating agent |
| EP2361964B1 (en) | 2010-02-25 | 2012-12-12 | The Procter & Gamble Company | Detergent composition |
| EP2380481B1 (en) | 2010-04-23 | 2014-12-31 | The Procter and Gamble Company | Automatic dishwashing product |
| ES2682051T3 (es) | 2010-04-23 | 2018-09-18 | The Procter & Gamble Company | Composición detergente |
| EP2380962B1 (en) | 2010-04-23 | 2016-03-30 | The Procter and Gamble Company | Particle |
| EP2380963B1 (en) | 2010-04-23 | 2015-12-23 | The Procter and Gamble Company | Method of perfuming |
| EP2380478A1 (en) | 2010-04-23 | 2011-10-26 | The Procter & Gamble Company | Automatic dishwashing product |
| EP2383329A1 (en) | 2010-04-23 | 2011-11-02 | The Procter & Gamble Company | Particle |
| MX339810B (es) | 2010-05-06 | 2016-06-09 | The Procter & Gamble Company * | Productos de consumo con variantes de la proteasa. |
| RU2552624C2 (ru) | 2010-08-17 | 2015-06-10 | Дзе Проктер Энд Гэмбл Компани | Способ мытья посуды вручную со стойкой пеной |
| EP2420558B1 (en) | 2010-08-17 | 2017-08-02 | The Procter & Gamble Company | Stable sustainable hand dish-washing detergents |
| BR112013008347A2 (pt) | 2010-10-06 | 2016-06-14 | Bp Corp North America Inc | polipeptídeos de variantes cbh i |
| TR201901382T4 (tr) | 2011-05-05 | 2019-02-21 | Danisco Inc | Serin proteaz varyantlarını içeren bileşimler ve yöntemler. |
| WO2012151480A2 (en) | 2011-05-05 | 2012-11-08 | The Procter & Gamble Company | Compositions and methods comprising serine protease variants |
| US20140371435A9 (en) | 2011-06-03 | 2014-12-18 | Eduardo Torres | Laundry Care Compositions Containing Thiophene Azo Dyes |
| EP2537918A1 (en) | 2011-06-20 | 2012-12-26 | The Procter & Gamble Company | Consumer products with lipase comprising coated particles |
| EP2540824A1 (en) * | 2011-06-30 | 2013-01-02 | The Procter & Gamble Company | Cleaning compositions comprising amylase variants reference to a sequence listing |
| EP3798303A1 (en) | 2011-06-30 | 2021-03-31 | Novozymes A/S | Alpha-amylase-variants |
| MX351850B (es) | 2011-06-30 | 2017-10-31 | Novozymes As | Metodo para el cribado de alfa-amilasas. |
| EP2551335A1 (en) | 2011-07-25 | 2013-01-30 | The Procter & Gamble Company | Enzyme stabilized liquid detergent composition |
| ES2633292T3 (es) | 2011-10-19 | 2017-09-20 | The Procter & Gamble Company | Partícula |
| AU2012328562A1 (en) | 2011-10-28 | 2014-03-13 | Danisco Us Inc. | Variant maltohexaose-forming alpha-amylase variants |
| KR101882182B1 (ko) * | 2011-11-22 | 2018-07-27 | 삼성전자주식회사 | 식기 세척기 및 그 제어 방법 |
| MX353896B (es) | 2012-02-03 | 2018-02-01 | Procter & Gamble | Composiciones y metodos para el tratamiento de superficies con lipasas. |
| EP2828339B1 (en) | 2012-03-19 | 2018-10-31 | Milliken & Company | Carboxylate dyes |
| CN104204198B (zh) | 2012-04-02 | 2018-09-25 | 诺维信公司 | 脂肪酶变体以及编码其的多核苷酸 |
| EP2662436B1 (en) | 2012-05-11 | 2017-08-23 | The Procter & Gamble Company | Detergent composition |
| EP2674475A1 (en) | 2012-06-11 | 2013-12-18 | The Procter & Gamble Company | Detergent composition |
| US10246692B2 (en) | 2012-07-12 | 2019-04-02 | Novozymes A/S | Polypeptides having lipase activity and polynucleotides encoding same |
| ES2678543T3 (es) | 2012-08-24 | 2018-08-13 | The Procter & Gamble Company | Método de lavado de vajillas |
| ES2677702T3 (es) | 2012-08-24 | 2018-08-06 | The Procter & Gamble Company | Método de lavado de vajillas |
| JP2016506442A (ja) | 2012-12-20 | 2016-03-03 | ザ プロクター アンド ギャンブルカンパニー | ケイ酸塩でコーティングされた漂白剤を含む洗剤組成物 |
| EP2746381A1 (en) | 2012-12-21 | 2014-06-25 | The Procter & Gamble Company | Cleaning pack |
| US9902946B2 (en) * | 2013-01-03 | 2018-02-27 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
| JP6251296B2 (ja) | 2013-03-05 | 2017-12-20 | ザ プロクター アンド ギャンブル カンパニー | 混合糖組成物 |
| DK3336183T4 (da) * | 2013-03-11 | 2025-06-10 | Danisco Us Inc | Kombinatoriske alfa-amylasevarianter |
| MX360759B (es) | 2013-03-21 | 2018-11-15 | Novozymes As | Polipeptidos con actividades lipasa y polinucleotidos que los codifican. |
| WO2014160820A1 (en) | 2013-03-28 | 2014-10-02 | The Procter & Gamble Company | Cleaning compositions containing a polyetheramine |
| WO2014183920A1 (en) * | 2013-05-17 | 2014-11-20 | Novozymes A/S | Polypeptides having alpha amylase activity |
| CN105229116A (zh) | 2013-05-28 | 2016-01-06 | 宝洁公司 | 包含光致变色染料的表面处理组合物 |
| JP2016526880A (ja) | 2013-05-29 | 2016-09-08 | ダニスコ・ユーエス・インク | 新規メタロプロテアーゼ |
| EP3260538B1 (en) | 2013-05-29 | 2021-04-14 | Danisco US Inc. | Novel metalloproteases |
| WO2014194032A1 (en) | 2013-05-29 | 2014-12-04 | Danisco Us Inc. | Novel metalloproteases |
| US20160108387A1 (en) | 2013-05-29 | 2016-04-21 | Danisco Us Inc. | Novel metalloproteases |
| CN105324482A (zh) * | 2013-06-21 | 2016-02-10 | 诺维信公司 | 具有淀粉酶活性的多肽以及对其进行编码的多核苷酸 |
| WO2015042087A1 (en) | 2013-09-18 | 2015-03-26 | The Procter & Gamble Company | Laundry care composition comprising carboxylate dye |
| MX2016003538A (es) | 2013-09-18 | 2016-06-28 | Procter & Gamble | Composiciones para el cuidado de la ropa que contienen colorantes azoicos de tiofeno y carboxilato. |
| US9834682B2 (en) | 2013-09-18 | 2017-12-05 | Milliken & Company | Laundry care composition comprising carboxylate dye |
| CA2921432A1 (en) | 2013-09-18 | 2015-03-26 | The Procter & Gamble Company | Laundry care composition comprising carboxylate dye |
| EP2857486A1 (en) | 2013-10-07 | 2015-04-08 | WeylChem Switzerland AG | Multi-compartment pouch comprising cleaning compositions, washing process and use for washing and cleaning of textiles and dishes |
| EP2857485A1 (en) | 2013-10-07 | 2015-04-08 | WeylChem Switzerland AG | Multi-compartment pouch comprising alkanolamine-free cleaning compositions, washing process and use for washing and cleaning of textiles and dishes |
| EP2857487A1 (en) | 2013-10-07 | 2015-04-08 | WeylChem Switzerland AG | Multi-compartment pouch comprising cleaning compositions, washing process and use for washing and cleaning of textiles and dishes |
| CN106029881B (zh) | 2013-12-13 | 2023-01-10 | 丹尼斯科美国公司 | 吉氏芽孢杆菌-进化枝的丝氨酸蛋白酶 |
| EP3080262B1 (en) | 2013-12-13 | 2019-02-06 | Danisco US Inc. | Serine proteases of bacillus species |
| AU2014366222B2 (en) | 2013-12-16 | 2018-07-19 | Nutrition & Biosciences USA 4, Inc. | Use of poly alpha-1,3-glucan ethers as viscosity modifiers |
| AU2014364772B2 (en) | 2013-12-18 | 2018-07-26 | Nutrition & Biosciences USA 4, Inc. | Cationic poly alpha-1,3-glucan ethers |
| EP3097174A1 (en) | 2014-01-22 | 2016-11-30 | The Procter & Gamble Company | Method of treating textile fabrics |
| WO2015112339A1 (en) | 2014-01-22 | 2015-07-30 | The Procter & Gamble Company | Fabric treatment composition |
| WO2015112338A1 (en) | 2014-01-22 | 2015-07-30 | The Procter & Gamble Company | Method of treating textile fabrics |
| EP3097175B1 (en) | 2014-01-22 | 2018-10-17 | The Procter and Gamble Company | Fabric treatment composition |
| CA2841024C (en) | 2014-01-30 | 2017-03-07 | The Procter & Gamble Company | Unit dose article |
| US20150232785A1 (en) | 2014-02-14 | 2015-08-20 | E I Du Pont De Nemours And Company | Polysaccharides for viscosity modification |
| US10752562B2 (en) | 2014-02-25 | 2020-08-25 | The Procter & Gamble Company | Process for making renewable surfactant intermediates and surfactants from fats and oils and products thereof |
| WO2015130669A1 (en) | 2014-02-25 | 2015-09-03 | The Procter & Gamble Company | A process for making renewable surfactant intermediates and surfactants from fats and oils and products thereof |
| EP2915872A1 (en) | 2014-03-06 | 2015-09-09 | The Procter and Gamble Company | Dishwashing composition |
| EP2915873A1 (en) | 2014-03-06 | 2015-09-09 | The Procter and Gamble Company | Dishwashing composition |
| MX2016011467A (es) | 2014-03-11 | 2016-11-16 | Du Pont | Poli alfa-1,3-glucano oxidado como mejorador de detergente. |
| DK3119884T3 (da) | 2014-03-21 | 2019-10-14 | Danisco Us Inc | Serinproteaser af bacillus-arter |
| EP3122848A1 (en) | 2014-03-27 | 2017-02-01 | The Procter & Gamble Company | Cleaning compositions containing a polyetheramine |
| EP3122849B1 (en) | 2014-03-27 | 2021-07-21 | The Procter & Gamble Company | Cleaning compositions containing a polyetheramine |
| EP3122850A1 (en) | 2014-03-27 | 2017-02-01 | The Procter & Gamble Company | Cleaning compositions containing a polyetheramine |
| EP2924105A1 (en) | 2014-03-28 | 2015-09-30 | The Procter and Gamble Company | Water soluble unit dose article |
| EP2924107A1 (en) | 2014-03-28 | 2015-09-30 | The Procter and Gamble Company | Water soluble unit dose article |
| EP2940116B1 (en) | 2014-04-30 | 2018-10-17 | The Procter and Gamble Company | Detergent |
| BR112016025504B1 (pt) | 2014-05-06 | 2022-02-15 | Milliken & Company | Composições para cuidado de lavanderia e método para tratar artigos têxteis |
| EP3152288A1 (en) | 2014-06-06 | 2017-04-12 | The Procter & Gamble Company | Detergent composition comprising polyalkyleneimine polymers |
| EP3155096B1 (en) * | 2014-06-12 | 2021-03-17 | Novozymes A/S | Oxidation stable alpha-amylase variants |
| US9714403B2 (en) | 2014-06-19 | 2017-07-25 | E I Du Pont De Nemours And Company | Compositions containing one or more poly alpha-1,3-glucan ether compounds |
| US9771548B2 (en) | 2014-06-19 | 2017-09-26 | E I Du Pont De Nemours And Company | Compositions containing one or more poly alpha-1,3-glucan ether compounds |
| US9617502B2 (en) | 2014-09-15 | 2017-04-11 | The Procter & Gamble Company | Detergent compositions containing salts of polyetheramines and polymeric acid |
| US20160090552A1 (en) | 2014-09-25 | 2016-03-31 | The Procter & Gamble Company | Detergent compositions containing a polyetheramine and an anionic soil release polymer |
| US9487739B2 (en) | 2014-09-25 | 2016-11-08 | The Procter & Gamble Company | Cleaning compositions containing a polyetheramine |
| US9388368B2 (en) | 2014-09-26 | 2016-07-12 | The Procter & Gamble Company | Cleaning compositions containing a polyetheramine |
| DK3207129T3 (da) | 2014-10-17 | 2020-02-24 | Danisco Us Inc | Serinproteaser af bacillus-arten |
| DK3212662T3 (da) | 2014-10-27 | 2020-07-20 | Danisco Us Inc | Serinproteaser |
| EP4403631A3 (en) | 2014-10-27 | 2024-10-30 | Danisco US Inc. | Serine proteases |
| US20170335306A1 (en) | 2014-10-27 | 2017-11-23 | Danisco Us Inc. | Serine proteases |
| EP3224357A1 (en) | 2014-10-27 | 2017-10-04 | Danisco US Inc. | Serine proteases of bacillus species |
| EP3550017B1 (en) | 2014-10-27 | 2021-07-14 | Danisco US Inc. | Serine proteases |
| MX2017006377A (es) | 2014-11-17 | 2017-08-21 | Procter & Gamble | Composiciones de suministro de agentes beneficos. |
| ES2690335T3 (es) | 2014-11-26 | 2018-11-20 | The Procter & Gamble Company | Bolsa limpiadora |
| EP3026099B1 (en) | 2014-11-26 | 2021-02-17 | The Procter and Gamble Company | Cleaning pouch |
| ES2690336T3 (es) | 2014-11-26 | 2018-11-20 | The Procter & Gamble Company | Bolsa limpiadora |
| EP3026102B1 (en) | 2014-11-26 | 2018-12-26 | The Procter and Gamble Company | Cleaning pouch |
| EP3034596B2 (en) | 2014-12-17 | 2021-11-10 | The Procter & Gamble Company | Detergent composition |
| EP3034591A1 (en) | 2014-12-17 | 2016-06-22 | The Procter and Gamble Company | Method of automatic dishwashing |
| EP3034592A1 (en) | 2014-12-17 | 2016-06-22 | The Procter and Gamble Company | Method of automatic dishwashing |
| EP3034589A1 (en) | 2014-12-17 | 2016-06-22 | The Procter and Gamble Company | Detergent composition |
| EP3034590A1 (en) | 2014-12-17 | 2016-06-22 | The Procter and Gamble Company | Method of automatic dishwashing |
| EP3034597A1 (en) | 2014-12-17 | 2016-06-22 | The Procter and Gamble Company | Detergent composition |
| ES2733300T3 (es) | 2014-12-17 | 2019-11-28 | Procter & Gamble | Composición detergente |
| ES2668504T3 (es) | 2014-12-22 | 2018-05-18 | The Procter & Gamble Company | Proceso para reciclar bolsas de detergente |
| AU2015369965B2 (en) | 2014-12-23 | 2020-01-30 | Nutrition & Biosciences USA 4, Inc. | Enzymatically produced cellulose |
| EP3050955B2 (en) | 2015-02-02 | 2023-11-08 | The Procter & Gamble Company | Detergent pack |
| EP3050947A1 (en) | 2015-02-02 | 2016-08-03 | The Procter and Gamble Company | Detergent pack |
| EP3050954A1 (en) | 2015-02-02 | 2016-08-03 | The Procter and Gamble Company | New use of sulfonated polymers |
| EP3050950B1 (en) | 2015-02-02 | 2018-09-19 | The Procter and Gamble Company | New use of sulfonated polymers |
| ES2714130T3 (es) | 2015-02-02 | 2019-05-27 | Procter & Gamble | Composición detergente |
| EP3050951A1 (en) | 2015-02-02 | 2016-08-03 | The Procter and Gamble Company | Method of dishwashing |
| EP3050948B1 (en) | 2015-02-02 | 2018-09-19 | The Procter and Gamble Company | New use of complexing agent |
| EP3050952A1 (en) | 2015-02-02 | 2016-08-03 | The Procter and Gamble Company | Method of dishwashing |
| PL3088502T3 (pl) | 2015-04-29 | 2018-10-31 | The Procter & Gamble Company | Sposób obróbki tkaniny |
| CN117683589A (zh) | 2015-04-29 | 2024-03-12 | 宝洁公司 | 洗涤织物的方法 |
| JP2018517803A (ja) | 2015-04-29 | 2018-07-05 | ザ プロクター アンド ギャンブル カンパニー | 布地の処理方法 |
| ES2682178T3 (es) | 2015-04-29 | 2018-09-19 | The Procter & Gamble Company | Composición detergente |
| US20160319225A1 (en) | 2015-04-29 | 2016-11-03 | The Procter & Gamble Company | Method of treating a fabric |
| WO2016178668A1 (en) | 2015-05-04 | 2016-11-10 | Milliken & Company | Leuco triphenylmethane colorants as bluing agents in laundry care compositions |
| CA2981409A1 (en) | 2015-05-08 | 2016-11-17 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
| US10316275B2 (en) | 2015-05-08 | 2019-06-11 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
| JP7274819B2 (ja) | 2015-05-13 | 2023-05-17 | ダニスコ・ユーエス・インク | ＡｐｒＬ－ＣＬＡＤＥプロテアーゼ変異体及びその使用 |
| EP3101102B2 (en) | 2015-06-05 | 2023-12-13 | The Procter & Gamble Company | Compacted liquid laundry detergent composition |
| EP3101107B1 (en) | 2015-06-05 | 2019-04-24 | The Procter and Gamble Company | Compacted liquid laundry detergent composition |
| EP3101100B1 (en) | 2015-06-05 | 2018-02-07 | The Procter and Gamble Company | Compacted liquid laundry detergent composition |
| EP3101103B1 (en) | 2015-06-05 | 2019-04-24 | The Procter and Gamble Company | Compacted liquid laundry detergent composition |
| WO2016201040A1 (en) | 2015-06-09 | 2016-12-15 | Danisco Us Inc. | Water-triggered enzyme suspension |
| DK3307427T3 (da) | 2015-06-09 | 2023-11-06 | Danisco Us Inc | Osmotisk sprængnings-kapsler |
| WO2016201069A1 (en) | 2015-06-09 | 2016-12-15 | Danisco Us Inc | Low-density enzyme-containing particles |
| US11499146B2 (en) | 2015-06-17 | 2022-11-15 | Danisco Us Inc. | Bacillus gibsonii-clade serine proteases |
| PL3124587T3 (pl) | 2015-07-29 | 2019-08-30 | The Procter And Gamble Company | Wielofazowy produkt czyszczący w postaci dawki jednostkowej |
| EP3124586A1 (en) | 2015-07-29 | 2017-02-01 | The Procter and Gamble Company | Process for reducing malodour in a pack |
| US20180320158A1 (en) | 2015-11-05 | 2018-11-08 | Danisco Us Inc. | Paenibacillus and bacillus spp. mannanases |
| WO2017079751A1 (en) | 2015-11-05 | 2017-05-11 | Danisco Us Inc | Paenibacillus sp. mannanases |
| EP3374401B1 (en) | 2015-11-13 | 2022-04-06 | Nutrition & Biosciences USA 4, Inc. | Glucan fiber compositions for use in laundry care and fabric care |
| US10876074B2 (en) | 2015-11-13 | 2020-12-29 | Dupont Industrial Biosciences Usa, Llc | Glucan fiber compositions for use in laundry care and fabric care |
| US10844324B2 (en) | 2015-11-13 | 2020-11-24 | Dupont Industrial Biosciences Usa, Llc | Glucan fiber compositions for use in laundry care and fabric care |
| EP3173467A1 (en) | 2015-11-26 | 2017-05-31 | The Procter & Gamble Company | Cleaning compositions comprising enzymes |
| EP3178917A1 (en) | 2015-12-08 | 2017-06-14 | The Procter and Gamble Company | Cleaning pouch |
| WO2017100720A1 (en) | 2015-12-09 | 2017-06-15 | Danisco Us Inc. | Alpha-amylase combinatorial variants |
| EP3390625B1 (en) | 2015-12-18 | 2023-09-06 | Danisco US Inc. | Polypeptides with endoglucanase activity and uses thereof |
| CN119120430A (zh) | 2015-12-30 | 2024-12-13 | 诺维信公司 | 酶变体以及编码它们的多核苷酸 |
| ES2802454T3 (es) | 2016-04-08 | 2021-01-19 | Procter & Gamble | Composición de limpieza para lavavajillas |
| EP3228686B1 (en) | 2016-04-08 | 2021-10-27 | The Procter & Gamble Company | Automatic dishwashing |
| CA3018930A1 (en) | 2016-04-08 | 2017-10-12 | The Procter & Gamble Company | Automatic dishwashing cleaning composition |
| EP3228687B1 (en) | 2016-04-08 | 2019-05-22 | The Procter and Gamble Company | Dishwashing cleaning composition |
| DK179660B1 (en) * | 2016-04-08 | 2019-03-13 | Novozymes A/S | Stabilized Alpha-Amylase Variants and use of the same |
| CN105802940B (zh) * | 2016-04-18 | 2019-04-16 | 广西大学 | 一种地衣芽胞杆菌高温α-淀粉酶突变体及其应用 |
| EP3241889B1 (en) | 2016-05-03 | 2019-03-20 | The Procter and Gamble Company | Cleaning composition |
| EP3241890B1 (en) | 2016-05-03 | 2019-06-26 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
| CN109715791B (zh) | 2016-05-03 | 2023-07-14 | 丹尼斯科美国公司 | 蛋白酶变体及其用途 |
| US20170321157A1 (en) | 2016-05-03 | 2017-11-09 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
| EP3241891B1 (en) | 2016-05-03 | 2019-04-03 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
| US20190136218A1 (en) | 2016-05-05 | 2019-05-09 | Danisco Us Inc | Protease variants and uses thereof |
| MX2018014768A (es) | 2016-05-31 | 2019-03-06 | Danisco Us Inc | Variantes de proteasa y sus usos. |
| EP3257931A1 (en) | 2016-06-17 | 2017-12-20 | The Procter and Gamble Company | Detergent composition |
| WO2017219011A1 (en) | 2016-06-17 | 2017-12-21 | Danisco Us Inc | Protease variants and uses thereof |
| EP3266860B1 (en) | 2016-07-08 | 2020-04-08 | The Procter and Gamble Company | Process for making a particle |
| EP3275985A1 (en) | 2016-07-26 | 2018-01-31 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
| EP3275986B1 (en) | 2016-07-26 | 2020-07-08 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
| EP3275988B1 (en) | 2016-07-26 | 2020-07-08 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
| EP3275987A1 (en) | 2016-07-26 | 2018-01-31 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
| EP3275989A1 (en) | 2016-07-26 | 2018-01-31 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
| EP3290503A3 (en) | 2016-09-01 | 2018-05-30 | The Procter & Gamble Company | Automatic dishwashing cleaning composition |
| EP3312265A1 (en) | 2016-10-18 | 2018-04-25 | The Procter and Gamble Company | Detergent composition |
| US20180119070A1 (en) | 2016-11-01 | 2018-05-03 | The Procter & Gamble Company | Leuco colorants as bluing agents in laundry care compositions, packaging, kits and methods thereof |
| WO2018085390A1 (en) | 2016-11-01 | 2018-05-11 | Milliken & Company | Leuco colorants as bluing agents in laundry care compositions |
| US12049608B2 (en) | 2016-11-01 | 2024-07-30 | The Procter & Gamble Company | Leuco colorants as bluing agents in laundry care compositions |
| US20180119056A1 (en) | 2016-11-03 | 2018-05-03 | Milliken & Company | Leuco Triphenylmethane Colorants As Bluing Agents in Laundry Care Compositions |
| US20190264138A1 (en) | 2016-11-07 | 2019-08-29 | Danisco Us Inc. | Laundry detergent composition |
| US10550443B2 (en) | 2016-12-02 | 2020-02-04 | The Procter & Gamble Company | Cleaning compositions including enzymes |
| EP4001389A1 (en) | 2016-12-02 | 2022-05-25 | The Procter & Gamble Company | Cleaning compositions including enzymes |
| MX2019006422A (es) | 2016-12-02 | 2019-08-01 | Procter & Gamble | Composiciones de limpieza que incluyen enzimas. |
| US11946081B2 (en) | 2016-12-21 | 2024-04-02 | Danisco Us Inc. | Bacillus gibsonii-clade serine proteases |
| DK3559227T3 (da) | 2016-12-21 | 2025-05-05 | Danisco Us Inc | Proteasevarianter og anvendelser deraf |
| EP3339423A1 (en) | 2016-12-22 | 2018-06-27 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
| EP3339410A1 (en) | 2016-12-22 | 2018-06-27 | The Procter & Gamble Company | Automatic dishwashing composition |
| FI3357994T3 (fi) | 2017-02-01 | 2023-11-22 | Procter & Gamble | Amylaasivariantteja käsittäviä puhdistuskoostumuksia |
| EP3583210B1 (en) | 2017-03-15 | 2021-07-07 | Danisco US Inc. | Trypsin-like serine proteases and uses thereof |
| EP3601515A1 (en) | 2017-03-31 | 2020-02-05 | Danisco US Inc. | Delayed release enzyme formulations for bleach-containing detergents |
| US20210095268A1 (en) | 2017-03-31 | 2021-04-01 | Danisco Us Inc | Alpha-amylase combinatorial variants |
| EP3415592A1 (en) | 2017-06-15 | 2018-12-19 | The Procter & Gamble Company | Water-soluble unit dose article comprising a solid laundry detergent composition |
| EP3418366A1 (en) | 2017-06-19 | 2018-12-26 | The Procter & Gamble Company | Automatic dishwashing cleaning composition |
| EP3418364A1 (en) | 2017-06-19 | 2018-12-26 | The Procter & Gamble Company | Automatic dishwashing cleaning composition |
| EP3418365A1 (en) | 2017-06-19 | 2018-12-26 | The Procter & Gamble Company | Automatic dishwashing cleaning composition |
| CN110809624A (zh) | 2017-06-30 | 2020-02-18 | 丹尼斯科美国公司 | 低团聚的含酶颗粒 |
| EP3441450A1 (en) | 2017-08-11 | 2019-02-13 | The Procter & Gamble Company | Automatic dishwashing composition |
| US11441139B2 (en) | 2017-08-18 | 2022-09-13 | Danisco Us Inc (157111) | α-Amylase variants |
| EP3456808A1 (en) | 2017-09-13 | 2019-03-20 | The Procter & Gamble Company | Automatic dishwashing cleaning composition |
| EP3467085A1 (en) | 2017-10-05 | 2019-04-10 | The Procter & Gamble Company | Dishwashing cleaning composition |
| ES2874024T3 (es) | 2017-10-05 | 2021-11-04 | Procter & Gamble | Composición de limpieza para el lavado de vajillas |
| EP3694972A1 (en) | 2017-10-12 | 2020-08-19 | The Procter and Gamble Company | Leuco colorants as bluing agents in laundry care composition |
| JP6945947B2 (ja) | 2017-10-12 | 2021-10-06 | ザ プロクター アンド ギャンブル カンパニーＴｈｅ Ｐｒｏｃｔｅｒ ＆ Ｇａｍｂｌｅ Ｃｏｍｐａｎｙ | 洗濯ケア組成物中の青味剤として第２のホワイトニング剤と組み合わせたロイコ着色剤 |
| TWI715878B (zh) | 2017-10-12 | 2021-01-11 | 美商美力肯及公司 | 隱色著色劑及組成物 |
| US20190194579A1 (en) | 2017-10-12 | 2019-06-27 | The Procter & Gamble Company | Leuco colorants as bluing agents in laundry care compositions |
| BR112020008476B1 (pt) | 2017-11-01 | 2023-11-21 | Milliken & Company | Composto leuco |
| CN111373039A (zh) | 2017-11-29 | 2020-07-03 | 丹尼斯科美国公司 | 具有改善的稳定性的枯草杆菌蛋白酶变体 |
| MX2020006518A (es) | 2017-12-21 | 2020-10-28 | Danisco Us Inc | Gránulos de fusión en caliente, que contienen enzimas, que comprenden un desecante termotolerante. |
| WO2019156670A1 (en) | 2018-02-08 | 2019-08-15 | Danisco Us Inc. | Thermally-resistant wax matrix particles for enzyme encapsulation |
| EP3530723B1 (en) | 2018-02-21 | 2023-03-29 | The Procter & Gamble Company | Automatic dishwashing composition |
| EP3799601A1 (en) | 2018-06-19 | 2021-04-07 | Danisco US Inc. | Subtilisin variants |
| WO2019245704A1 (en) | 2018-06-19 | 2019-12-26 | Danisco Us Inc | Subtilisin variants |
| WO2020028443A1 (en) | 2018-07-31 | 2020-02-06 | Danisco Us Inc | Variant alpha-amylases having amino acid substitutions that lower the pka of the general acid |
| EP3844255A1 (en) | 2018-08-30 | 2021-07-07 | Danisco US Inc. | Enzyme-containing granules |
| WO2020068486A1 (en) | 2018-09-27 | 2020-04-02 | Danisco Us Inc | Compositions for medical instrument cleaning |
| CN113166745B (zh) | 2018-10-12 | 2025-07-18 | 丹尼斯科美国公司 | 在螯合剂存在下具有可增强稳定性的突变的α-淀粉酶 |
| EP3887515A1 (en) | 2018-11-28 | 2021-10-06 | Danisco US Inc. | Subtilisin variants having improved stability |
| WO2020186052A1 (en) | 2019-03-14 | 2020-09-17 | The Procter & Gamble Company | Method for treating cotton |
| EP3938484A1 (en) | 2019-03-14 | 2022-01-19 | The Procter & Gamble Company | Cleaning compositions comprising enzymes |
| EP3938502A1 (en) | 2019-03-14 | 2022-01-19 | The Procter & Gamble Company | Cleaning compositions comprising enzymes |
| EP3741283B1 (en) | 2019-05-22 | 2025-03-19 | The Procter & Gamble Company | Automatic dishwashing method |
| WO2020242858A1 (en) | 2019-05-24 | 2020-12-03 | Danisco Us Inc | Subtilisin variants and methods of use |
| EP3980517A1 (en) | 2019-06-06 | 2022-04-13 | Danisco US Inc. | Methods and compositions for cleaning |
| EP3760699A1 (en) | 2019-07-02 | 2021-01-06 | The Procter & Gamble Company | Automatic dishwashing detergent composition |
| CN114846023B (zh) | 2019-10-24 | 2025-10-31 | 丹尼斯科美国公司 | 成麦芽五糖/麦芽六糖变体α-淀粉酶 |
| EP3835396B1 (en) | 2019-12-09 | 2025-12-03 | The Procter & Gamble Company | A detergent composition comprising a polymer |
| CN111471668B (zh) * | 2020-02-28 | 2022-05-24 | 浙江工业大学 | 一种腈水解酶突变体及其在制备1-氰基环己基乙酸中的应用 |
| DE102020205400A1 (de) | 2020-04-29 | 2021-11-04 | Henkel Ag & Co. Kgaa | Hochalkalisches Textilwaschmittel mit Protease |
| DE102020205381A1 (de) | 2020-04-29 | 2021-11-04 | Henkel Ag & Co. Kgaa | Hochalkalisches Textilwaschmittel mit Protease |
| CN115551978A (zh) | 2020-06-05 | 2022-12-30 | 宝洁公司 | 含有支链表面活性剂的洗涤剂组合物 |
| EP4204553A1 (en) | 2020-08-27 | 2023-07-05 | Danisco US Inc. | Enzymes and enzyme compositions for cleaning |
| EP3974504B1 (en) | 2020-09-29 | 2023-07-26 | The Procter & Gamble Company | Automatic dishwashing cleaning composition |
| CN116507725A (zh) * | 2020-10-07 | 2023-07-28 | 诺维信公司 | α-淀粉酶变体 |
| WO2022093189A1 (en) | 2020-10-27 | 2022-05-05 | Milliken & Company | Compositions comprising leuco compounds and colorants |
| CA3196371A1 (en) | 2020-10-29 | 2022-05-05 | Catherine Jones | Cleaning composition comprising alginate lyase enzymes |
| CN116472331B (zh) | 2020-11-17 | 2025-09-02 | 宝洁公司 | 自动盘碟洗涤组合物 |
| EP4006131A1 (en) | 2020-11-30 | 2022-06-01 | The Procter & Gamble Company | Method of laundering fabric |
| EP4012011A1 (de) | 2020-12-14 | 2022-06-15 | Henkel AG & Co. KGaA | Reinigungsmittel insbesondere für eine küchenmaschine |
| EP4011256A1 (de) | 2020-12-14 | 2022-06-15 | Henkel AG & Co. KGaA | Verfahren zur reinigung einer elektromotorisch betriebenen küchenmaschine |
| DE102021213462A1 (de) | 2021-11-30 | 2023-06-01 | Henkel Ag & Co. Kgaa | Verfahren zur Reinigung einer elektromotorisch betriebenen Küchenmaschine |
| WO2022128620A1 (de) | 2020-12-14 | 2022-06-23 | Henkel Ag & Co. Kgaa | Verfahren zur reinigung einer elektromotorisch betriebenen küchenmaschine |
| US20240052000A1 (en) | 2020-12-15 | 2024-02-15 | Novozymes A/S | Mutated host cells with reduced cell motility on |
| CN116529285B (zh) | 2020-12-23 | 2025-05-13 | 巴斯夫股份公司 | 两亲性烷氧基化多胺及其用途 |
| US20240117275A1 (en) | 2021-01-29 | 2024-04-11 | Danisco Us Inc. | Compositions for cleaning and methods related thereto |
| CN116615536A (zh) | 2021-03-15 | 2023-08-18 | 宝洁公司 | 含有多肽变体的清洁组合物 |
| US20240218300A1 (en) | 2021-03-26 | 2024-07-04 | Novozymes A/S | Detergent composition with reduced polymer content |
| CN117157382A (zh) | 2021-05-05 | 2023-12-01 | 宝洁公司 | 制备清洁组合物及检测污垢的方法 |
| EP4086330A1 (en) | 2021-05-06 | 2022-11-09 | The Procter & Gamble Company | Surface treatment |
| EP4108767A1 (en) | 2021-06-22 | 2022-12-28 | The Procter & Gamble Company | Cleaning or treatment compositions containing nuclease enzymes |
| ES2999112T3 (en) | 2021-06-22 | 2025-02-24 | Procter & Gamble | A method of treating dishware in a domestic automatic dishwashing machine |
| EP4363565A1 (en) | 2021-06-30 | 2024-05-08 | Danisco US Inc. | Variant lipases and uses thereof |
| EP4123006A1 (en) | 2021-07-19 | 2023-01-25 | The Procter & Gamble Company | Composition comprising spores and pro-perfume materials |
| EP4123007A1 (en) | 2021-07-19 | 2023-01-25 | The Procter & Gamble Company | Fabric treatment using bacterial spores |
| EP4386035A4 (en) | 2021-08-10 | 2025-07-30 | Nippon Catalytic Chem Ind | POLYALKYLENE OXIDE-CONTAINING COMPOUND |
| WO2023034486A2 (en) | 2021-09-03 | 2023-03-09 | Danisco Us Inc. | Laundry compositions for cleaning |
| CN117957318A (zh) | 2021-09-13 | 2024-04-30 | 丹尼斯科美国公司 | 含有生物活性物质的颗粒 |
| WO2023064749A1 (en) | 2021-10-14 | 2023-04-20 | The Procter & Gamble Company | A fabric and home care product comprising cationic soil release polymer and lipase enzyme |
| EP4194536A1 (en) | 2021-12-08 | 2023-06-14 | The Procter & Gamble Company | Laundry treatment cartridge |
| EP4194537A1 (en) | 2021-12-08 | 2023-06-14 | The Procter & Gamble Company | Laundry treatment cartridge |
| WO2023104846A1 (en) | 2021-12-10 | 2023-06-15 | Novozymes A/S | Improved protein production in recombinant bacteria |
| WO2023114939A2 (en) | 2021-12-16 | 2023-06-22 | Danisco Us Inc. | Subtilisin variants and methods of use |
| WO2023114932A2 (en) | 2021-12-16 | 2023-06-22 | Danisco Us Inc. | Subtilisin variants and methods of use |
| US20250051748A1 (en) | 2021-12-16 | 2025-02-13 | Danisco Us Inc. | Subtilisin variants and methods of use |
| CN118647716A (zh) | 2021-12-16 | 2024-09-13 | 丹尼斯科美国公司 | 成麦芽五糖/麦芽六糖变体α-淀粉酶 |
| WO2023168234A1 (en) | 2022-03-01 | 2023-09-07 | Danisco Us Inc. | Enzymes and enzyme compositions for cleaning |
| EP4273209A1 (en) | 2022-05-04 | 2023-11-08 | The Procter & Gamble Company | Machine-cleaning compositions containing enzymes |
| EP4273210A1 (en) | 2022-05-04 | 2023-11-08 | The Procter & Gamble Company | Detergent compositions containing enzymes |
| EP4525615A2 (en) | 2022-05-14 | 2025-03-26 | Novozymes A/S | Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections |
| EP4279571A1 (en) | 2022-05-19 | 2023-11-22 | The Procter & Gamble Company | Laundry composition comprising spores |
| EP4286501B1 (en) | 2022-06-01 | 2025-01-08 | The Procter & Gamble Company | Dishwashing detergent composition comprising xylanase and block co-polymer |
| DE102022205588A1 (de) | 2022-06-01 | 2023-12-07 | Henkel Ag & Co. Kgaa | Wasch- und reinigungsmittel mit verbesserter enzymstabilität |
| DE102022205594A1 (de) | 2022-06-01 | 2023-12-07 | Henkel Ag & Co. Kgaa | Leistungsverbesserte und lagerstabile protease-varianten |
| DE102022205591A1 (de) | 2022-06-01 | 2023-12-07 | Henkel Ag & Co. Kgaa | Wasch- und reinigungsmittel mit verbesserter enzymstabilität |
| DE102022205593A1 (de) | 2022-06-01 | 2023-12-07 | Henkel Ag & Co. Kgaa | Wasch- und reinigungsmittel mit verbesserter enzymstabilität |
| EP4286499A1 (en) | 2022-06-01 | 2023-12-06 | The Procter & Gamble Company | Dishwashing detergent composition comprising xylanase and sulphonated carboxylate polymer |
| EP4286500A1 (en) | 2022-06-01 | 2023-12-06 | The Procter & Gamble Company | Use of xylanase in a dishwashing process |
| CN119487167A (zh) | 2022-06-21 | 2025-02-18 | 丹尼斯科美国公司 | 用于清洁的包含具有嗜热菌蛋白酶活性的多肽的组合物和方法 |
| CN115975990B (zh) * | 2022-07-18 | 2024-04-30 | 青岛润博特生物科技有限公司 | 一种高比活中温淀粉酶突变体 |
| EP4321604A1 (en) | 2022-08-08 | 2024-02-14 | The Procter & Gamble Company | A fabric and home care composition comprising surfactant and a polyester |
| EP4324900A1 (en) | 2022-08-17 | 2024-02-21 | Henkel AG & Co. KGaA | Detergent composition comprising enzymes |
| WO2024050339A1 (en) | 2022-09-02 | 2024-03-07 | Danisco Us Inc. | Mannanase variants and methods of use |
| JP2025529133A (ja) | 2022-09-02 | 2025-09-04 | ダニスコ・ユーエス・インク | サブチリシン変異体及びそれに関連する方法 |
| CA3265943A1 (en) | 2022-09-02 | 2024-03-07 | Danisco Us Inc. | DETERGENT COMPOSITIONS AND ASSOCIATED PROCESSES |
| US20240090740A1 (en) | 2022-09-16 | 2024-03-21 | The Procter & Gamble Company | Methods and apparatuses for automatic dishwashing chemical distribution |
| EP4361061A1 (en) | 2022-10-26 | 2024-05-01 | The Procter & Gamble Company | Detergent product |
| EP4612209A1 (en) | 2022-11-04 | 2025-09-10 | The Procter & Gamble Company | Fabric and home care composition |
| EP4612207A1 (en) | 2022-11-04 | 2025-09-10 | Clariant International Ltd | Polyesters |
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Family Cites Families (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3912590A (en) | 1973-01-03 | 1975-10-14 | Novo Industri As | Procedure for liquefying starch |
| JPS57174089A (en) | 1981-04-20 | 1982-10-26 | Novo Industri As | Chain dividing enzyme product |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| DK122686D0 (da) | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| DK311186D0 (da) | 1986-06-30 | 1986-06-30 | Novo Industri As | Enzymer |
| FI89076C (fi) | 1986-07-09 | 1993-08-10 | Novo Nordisk As | Alfa-amylasblandningar foer att oeverfoera staerkelse i vaetskeform och foerfarande foer oeverfoering av staerkelse i flytande form |
| DE3909096A1 (de) | 1989-03-20 | 1990-09-27 | Garabed Antranikian | Alpha-amylase |
| DK0410498T3 (da) * | 1989-06-29 | 1999-03-22 | Genencor Int | Mutante mikrobielle alfa-amylaser med øget termisk, syre og/eller alkalisk stabilitet |
| JP3110452B2 (ja) | 1990-05-09 | 2000-11-20 | ノボ ノルディスク アクティーゼルスカブ | エンドグルカナーゼ酵素を含んでなるセルラーゼ調製物 |
| CA2155831C (en) | 1993-02-11 | 2009-11-10 | Richard L. Antrim | Oxidatively stable alpha-amylase |
| CN1189558C (zh) | 1993-10-08 | 2005-02-16 | 诺沃奇梅兹有限公司 | 淀粉酶变体 |
| MX196038B (es) | 1994-03-29 | 2000-04-14 | Novo Nordisk As | Amilasa de bacillus alcalino. |
| ATE301719T1 (de) * | 1994-06-17 | 2005-08-15 | Genencor Int | Von der alpha-amylase aus b. licheniformis abgeleitete amylolytische enzyme mit verbesserten eigenschaften |
| US6440716B1 (en) * | 1995-02-03 | 2002-08-27 | Novozymes A/S | α-amylase mutants |
| AR000862A1 (es) | 1995-02-03 | 1997-08-06 | Novozymes As | Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del |
| DK2199378T3 (da) | 1995-02-03 | 2012-10-29 | Novozymes As | alfa-amylase mutanter |
| DE19503621A1 (de) | 1995-02-03 | 1996-08-08 | Bosch Gmbh Robert | Hubkolbenpumpe |
| US6093562A (en) * | 1996-02-05 | 2000-07-25 | Novo Nordisk A/S | Amylase variants |
| US5736499A (en) * | 1995-06-06 | 1998-04-07 | Genencor International, Inc. | Mutant A-amylase |
| CN1192780B (zh) | 1995-08-11 | 2010-08-04 | 诺沃奇梅兹有限公司 | 新的脂解酶 |
| ES2432519T3 (es) * | 1996-04-30 | 2013-12-04 | Novozymes A/S | Mutantes de alfa-amilasa |
| MY119088A (en) * | 1996-11-26 | 2005-03-31 | Sony Corp | Information input method, information input sheet, and information input apparatus |
| ES2436066T3 (es) * | 1997-10-13 | 2013-12-26 | Novozymes A/S | Mutantes de alfa-amilasa |
| KR19990059284A (ko) * | 1997-12-30 | 1999-07-26 | 서평원 | 디지탈 트렁크 접속부에서 톤 감시 및 에뮬레이션시스템 |
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1998
- 1998-10-30 CN CN200410061736A patent/CN100593034C/zh not_active Expired - Lifetime
- 1998-10-30 EP EP10180224A patent/EP2388267A1/en not_active Withdrawn
- 1998-10-30 JP JP2000519071A patent/JP4426094B2/ja not_active Expired - Lifetime
- 1998-10-30 BR BRPI9816290A patent/BRPI9816290B1/pt not_active IP Right Cessation
- 1998-10-30 AU AU97373/98A patent/AU9737398A/en not_active Abandoned
- 1998-10-30 AT AT98951291T patent/ATE490311T1/de not_active IP Right Cessation
- 1998-10-30 US US09/183,412 patent/US6204232B1/en not_active Expired - Lifetime
- 1998-10-30 ES ES10180200.7T patent/ES2515218T3/es not_active Expired - Lifetime
- 1998-10-30 CA CA2308119A patent/CA2308119C/en not_active Expired - Lifetime
- 1998-10-30 BR BRPI9813328A patent/BRPI9813328B1/pt active IP Right Grant
- 1998-10-30 EP EP10180200.7A patent/EP2386568B1/en not_active Expired - Lifetime
- 1998-10-30 EP EP10180215.5A patent/EP2386569B1/en not_active Expired - Lifetime
- 1998-10-30 CN CNB988108712A patent/CN1163597C/zh not_active Ceased
- 1998-10-30 EP EP98951291A patent/EP1027428B1/en not_active Expired - Lifetime
- 1998-10-30 WO PCT/DK1998/000471 patent/WO1999023211A1/en not_active Ceased
- 1998-10-30 DE DE69842027T patent/DE69842027D1/de not_active Expired - Lifetime
- 1998-10-30 CA CA2845178A patent/CA2845178A1/en not_active Abandoned
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- 2001-01-25 US US09/769,864 patent/US6673589B2/en not_active Expired - Lifetime
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2003
- 2003-09-19 US US10/665,667 patent/US20040038368A1/en not_active Abandoned
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2004
- 2004-11-04 US US10/980,923 patent/US20050084937A1/en not_active Abandoned
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2007
- 2007-12-10 US US11/953,532 patent/US20090263881A1/en not_active Abandoned
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2013
- 2013-03-05 US US13/785,190 patent/US20140017715A1/en not_active Abandoned
-
2014
- 2014-01-24 US US14/163,585 patent/US20140248685A1/en not_active Abandoned
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2016
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Also Published As
| Publication number | Publication date |
|---|---|
| EP2386569B1 (en) | 2014-08-06 |
| CN1550549A (zh) | 2004-12-01 |
| US20050084937A1 (en) | 2005-04-21 |
| EP2388267A1 (en) | 2011-11-23 |
| AU9737398A (en) | 1999-05-24 |
| CN1278298A (zh) | 2000-12-27 |
| DE69842027D1 (de) | 2011-01-13 |
| EP1027428A1 (en) | 2000-08-16 |
| US20010039253A1 (en) | 2001-11-08 |
| BRPI9816290B1 (pt) | 2016-10-11 |
| CA2845178A1 (en) | 1999-05-14 |
| EP2386568A1 (en) | 2011-11-16 |
| CN1163597C (zh) | 2004-08-25 |
| JP2001521739A (ja) | 2001-11-13 |
| US20090263881A1 (en) | 2009-10-22 |
| BR9813328A (pt) | 2000-08-22 |
| CA2308119C (en) | 2014-06-03 |
| ATE490311T1 (de) | 2010-12-15 |
| US6204232B1 (en) | 2001-03-20 |
| CN100593034C (zh) | 2010-03-03 |
| EP2386569A1 (en) | 2011-11-16 |
| WO1999023211A1 (en) | 1999-05-14 |
| US20160222365A1 (en) | 2016-08-04 |
| ES2515218T3 (es) | 2014-10-29 |
| US20140017715A1 (en) | 2014-01-16 |
| US20140248685A1 (en) | 2014-09-04 |
| EP2386568B1 (en) | 2014-08-06 |
| CA2308119A1 (en) | 1999-05-14 |
| US20040038368A1 (en) | 2004-02-26 |
| EP1027428B1 (en) | 2010-12-01 |
| BRPI9813328B1 (pt) | 2016-04-12 |
| US6673589B2 (en) | 2004-01-06 |
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