JP2018503388A - 組み換え宿主におけるステビオールグリコシドの生産 - Google Patents

組み換え宿主におけるステビオールグリコシドの生産 Download PDF

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Publication number
JP2018503388A
JP2018503388A JP2017540261A JP2017540261A JP2018503388A JP 2018503388 A JP2018503388 A JP 2018503388A JP 2017540261 A JP2017540261 A JP 2017540261A JP 2017540261 A JP2017540261 A JP 2017540261A JP 2018503388 A JP2018503388 A JP 2018503388A
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seq
steviol
polypeptide
cell
ent
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Pending
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JP2017540261A
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JP2018503388A5 (enExample
Inventor
オルソン，キム
シモン，エルネスト
ミッケルセン，ミカエル ダルガート
ミッケルセン，ミカエル ダルガート
カールセン，サイモン
ドーチン，ヴェロニク
チャン リム，スウィー
チャン リム，スウィー
ダリン，ルイス
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Danstar Ferment AG
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Evolva AG
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Publication of JP2018503388A publication Critical patent/JP2018503388A/ja
Publication of JP2018503388A5 publication Critical patent/JP2018503388A5/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/20—Carbocyclic rings
    • C07H15/24—Condensed ring systems having three or more rings
    • C07H15/256—Polyterpene radicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P15/00—Preparation of compounds containing at least three condensed carbocyclic rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Seasonings (AREA)
JP2017540261A 2015-01-30 2016-01-29 組み換え宿主におけるステビオールグリコシドの生産 Pending JP2018503388A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562110207P 2015-01-30 2015-01-30
US62/110,207 2015-01-30
PCT/EP2016/052007 WO2016120486A1 (en) 2015-01-30 2016-01-29 Production of steviol glycosides in recombinant hosts

Publications (2)

Publication Number Publication Date
JP2018503388A true JP2018503388A (ja) 2018-02-08
JP2018503388A5 JP2018503388A5 (enExample) 2019-03-14

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Family Applications (1)

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JP2017540261A Pending JP2018503388A (ja) 2015-01-30 2016-01-29 組み換え宿主におけるステビオールグリコシドの生産

Country Status (9)

Country Link
US (4) US10364450B2 (enExample)
EP (2) EP3250686A1 (enExample)
JP (1) JP2018503388A (enExample)
CN (1) CN108337892B (enExample)
AU (3) AU2016211124B2 (enExample)
BR (1) BR112017016338A2 (enExample)
CA (1) CA2973674A1 (enExample)
SG (1) SG11201705606PA (enExample)
WO (1) WO2016120486A1 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019517811A (ja) * 2016-06-17 2019-06-27 カーギル インコーポレイテッド 経口摂取のためのステビオール配糖体組成物または使用
JP2022513480A (ja) * 2018-12-12 2022-02-08 コナゲン インコーポレイテッド 変異型ステビオール配糖体の生合成製造

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AU2012294452C1 (en) 2011-08-08 2017-11-30 Danstar Ferment Ag Recombinant production of steviol glycosides
BR112015019160A2 (pt) 2013-02-11 2017-08-22 Dalgaard Mikkelsen Michael Produção de glicosídeos de esteviol em hospedeiros recombinantes
KR20170052647A (ko) 2014-09-09 2017-05-12 에볼바 에스아 재조합 숙주에서 스테비올 글리코시드의 생성
JP6855376B2 (ja) 2014-12-17 2021-04-07 カーギル インコーポレイテッド 経口摂取又は経口使用のためのステビオールグリコシド化合物、組成物、及びステビオールグリコシド溶解度を増強するための方法
AU2016211124B2 (en) 2015-01-30 2022-04-14 Danstar Ferment Ag Production of steviol glycosides in recombinant hosts
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EP3383201B1 (en) * 2015-11-30 2022-11-30 Cargill, Incorporated Steviol glycoside compositions for oral ingestion or use
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KR20180132696A (ko) 2016-04-13 2018-12-12 에볼바 에스아 재조합 숙주에서의 스테비올 글리코사이드의 생산
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KR102540615B1 (ko) * 2016-10-14 2023-06-08 코나겐 인크. 스테비올 글리코시드의 생합성 제조 및 이를 위한 방법
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WO2018083338A1 (en) 2016-11-07 2018-05-11 Evolva Sa Production of steviol glycosides in recombinant hosts
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MX2020009626A (es) * 2018-03-16 2021-05-27 Purecircle Usa Inc Glucosidos de esteviol de alta pureza.
WO2019237044A1 (en) * 2018-06-08 2019-12-12 Purecircle Usa Inc. High-purity steviol glycosides
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SG11202103802XA (en) * 2018-10-15 2021-05-28 Amyris Inc Stevia rebaudiana kaurenoic acid hydroxylase variants for high efficiency production of rebaudiosides
EP3877519B1 (en) 2018-11-09 2026-03-11 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
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WO2020227148A1 (en) * 2019-05-03 2020-11-12 Amyris, Inc. Kaurenoic acid 13-hydroxylase (kah) variants and uses thereof
CN112080480B (zh) * 2019-06-14 2023-01-03 中国科学院分子植物科学卓越创新中心 糖基转移酶突变体及其应用
CN110305855B (zh) * 2019-06-26 2022-03-22 昆明理工大学 天麻GeCPR基因及其应用
PH12022551477A1 (en) * 2019-12-31 2023-11-29 Air Protein Inc High protein food compositions
PE20230443A1 (es) 2020-03-02 2023-03-08 Air Protein Inc Composiciones estructuradas analogas a la carne con alto contenido de proteinas
CN113444131B (zh) * 2021-06-24 2022-10-21 鲁东大学 N-乙酰氨基葡萄糖类化合物及其制备方法和应用

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