JP2017537626A5 - - Google Patents
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- Publication number
- JP2017537626A5 JP2017537626A5 JP2017529610A JP2017529610A JP2017537626A5 JP 2017537626 A5 JP2017537626 A5 JP 2017537626A5 JP 2017529610 A JP2017529610 A JP 2017529610A JP 2017529610 A JP2017529610 A JP 2017529610A JP 2017537626 A5 JP2017537626 A5 JP 2017537626A5
- Authority
- JP
- Japan
- Prior art keywords
- guide rna
- synthetic guide
- linkage
- rna
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108020005004 Guide RNA Proteins 0.000 claims 19
- 238000012986 modification Methods 0.000 claims 15
- 230000004048 modification Effects 0.000 claims 15
- XUYJLQHKOGNDPB-UHFFFAOYSA-N phosphonoacetic acid Chemical compound OC(=O)CP(O)(O)=O XUYJLQHKOGNDPB-UHFFFAOYSA-N 0.000 claims 7
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 6
- OVONXEQGWXGFJD-UHFFFAOYSA-N 4-sulfanylidene-1h-pyrimidin-2-one Chemical compound SC=1C=CNC(=O)N=1 OVONXEQGWXGFJD-UHFFFAOYSA-N 0.000 claims 4
- 108091033319 polynucleotide Proteins 0.000 claims 4
- 102000040430 polynucleotide Human genes 0.000 claims 4
- 239000002157 polynucleotide Substances 0.000 claims 4
- 108090000623 proteins and genes Proteins 0.000 claims 4
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 3
- 239000007850 fluorescent dye Substances 0.000 claims 3
- 238000000034 method Methods 0.000 claims 3
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 claims 3
- KUUMDZYFBMADNM-UHFFFAOYSA-N 2-dihydroxyphosphinothioylacetic acid Chemical compound OC(=O)CP(O)(O)=S KUUMDZYFBMADNM-UHFFFAOYSA-N 0.000 claims 2
- ZAYHVCMSTBRABG-UHFFFAOYSA-N 5-Methylcytidine Natural products O=C1N=C(N)C(C)=CN1C1C(O)C(O)C(CO)O1 ZAYHVCMSTBRABG-UHFFFAOYSA-N 0.000 claims 2
- ZAYHVCMSTBRABG-JXOAFFINSA-N 5-methylcytidine Chemical compound O=C1N=C(N)C(C)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](CO)O1 ZAYHVCMSTBRABG-JXOAFFINSA-N 0.000 claims 2
- MSSXOMSJDRHRMC-UHFFFAOYSA-N 9H-purine-2,6-diamine Chemical compound NC1=NC(N)=C2NC=NC2=N1 MSSXOMSJDRHRMC-UHFFFAOYSA-N 0.000 claims 2
- 230000002708 enhancing effect Effects 0.000 claims 2
- 108020004707 nucleic acids Proteins 0.000 claims 2
- 102000039446 nucleic acids Human genes 0.000 claims 2
- 150000007523 nucleic acids Chemical class 0.000 claims 2
- 125000003729 nucleotide group Chemical group 0.000 claims 2
- WRADPCFZZWXOTI-BMRADRMJSA-N (9E)-10-nitrooctadecenoic acid Chemical compound CCCCCCCC\C([N+]([O-])=O)=C/CCCCCCCC(O)=O WRADPCFZZWXOTI-BMRADRMJSA-N 0.000 claims 1
- MKKUNUHCFOLYDK-LTNPLRIYSA-N 1-[(2r,3s,4r,5r)-3-fluoro-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione Chemical compound O=C1NC(=O)C(C)=CN1[C@H]1[C@](F)(O)[C@H](O)[C@@H](CO)O1 MKKUNUHCFOLYDK-LTNPLRIYSA-N 0.000 claims 1
- -1 2'-O-methyl Chemical compound 0.000 claims 1
- 108091033409 CRISPR Proteins 0.000 claims 1
- 238000010354 CRISPR gene editing Methods 0.000 claims 1
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 claims 1
- 108091028113 Trans-activating crRNA Proteins 0.000 claims 1
- 230000000295 complement effect Effects 0.000 claims 1
- 238000010362 genome editing Methods 0.000 claims 1
- 238000000338 in vitro Methods 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2022020615A JP7579820B2 (ja) | 2014-12-03 | 2022-02-14 | 化学修飾を有するガイドｒｎａ |
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462087211P | 2014-12-03 | 2014-12-03 | |
| US62/087,211 | 2014-12-03 | ||
| US201562146189P | 2015-04-10 | 2015-04-10 | |
| US62/146,189 | 2015-04-10 | ||
| US201562256095P | 2015-11-16 | 2015-11-16 | |
| US62/256,095 | 2015-11-16 | ||
| PCT/US2015/000143 WO2016089433A1 (en) | 2014-12-03 | 2015-12-03 | Guide rna with chemical modifications |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022020615A Division JP7579820B2 (ja) | 2014-12-03 | 2022-02-14 | 化学修飾を有するガイドｒｎａ |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017537626A JP2017537626A (ja) | 2017-12-21 |
| JP2017537626A5 true JP2017537626A5 (enExample) | 2019-01-17 |
| JP7068821B2 JP7068821B2 (ja) | 2022-05-17 |
Family
ID=56092183
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017529610A Active JP7068821B2 (ja) | 2014-12-03 | 2015-12-03 | 化学修飾を有するガイドｒｎａ |
| JP2022020615A Active JP7579820B2 (ja) | 2014-12-03 | 2022-02-14 | 化学修飾を有するガイドｒｎａ |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022020615A Active JP7579820B2 (ja) | 2014-12-03 | 2022-02-14 | 化学修飾を有するガイドｒｎａ |
Country Status (8)
| Country | Link |
|---|---|
| US (5) | US10900034B2 (enExample) |
| EP (2) | EP4400584A3 (enExample) |
| JP (2) | JP7068821B2 (enExample) |
| KR (2) | KR102629128B1 (enExample) |
| CN (1) | CN107250148B (enExample) |
| AU (1) | AU2015355546B2 (enExample) |
| CA (1) | CA2969619A1 (enExample) |
| WO (1) | WO2016089433A1 (enExample) |
Families Citing this family (185)
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-
2015
- 2015-12-03 CA CA2969619A patent/CA2969619A1/en active Pending
- 2015-12-03 WO PCT/US2015/000143 patent/WO2016089433A1/en not_active Ceased
- 2015-12-03 AU AU2015355546A patent/AU2015355546B2/en active Active
- 2015-12-03 JP JP2017529610A patent/JP7068821B2/ja active Active
- 2015-12-03 KR KR1020177018311A patent/KR102629128B1/ko active Active
- 2015-12-03 EP EP24165113.2A patent/EP4400584A3/en active Pending
- 2015-12-03 EP EP15866342.7A patent/EP3227447B1/en active Active
- 2015-12-03 KR KR1020247002012A patent/KR102763527B1/ko active Active
- 2015-12-03 US US14/757,204 patent/US10900034B2/en active Active
- 2015-12-03 CN CN201580075275.4A patent/CN107250148B/zh active Active
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2017
- 2017-05-26 US US15/607,295 patent/US10337001B2/en active Active
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- 2020-11-24 US US17/103,678 patent/US20210079389A1/en active Pending
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- 2023-06-09 US US18/332,464 patent/US20230416733A1/en not_active Abandoned
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