JP2015512611A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2015512611A5 JP2015512611A5 JP2014550709A JP2014550709A JP2015512611A5 JP 2015512611 A5 JP2015512611 A5 JP 2015512611A5 JP 2014550709 A JP2014550709 A JP 2014550709A JP 2014550709 A JP2014550709 A JP 2014550709A JP 2015512611 A5 JP2015512611 A5 JP 2015512611A5
- Authority
- JP
- Japan
- Prior art keywords
- trichoderma
- reduced
- cell
- protease activity
- proteases
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108091005804 Peptidases Proteins 0.000 claims 23
- 239000004365 Protease Substances 0.000 claims 23
- 241000223259 Trichoderma Species 0.000 claims 19
- 230000000694 effects Effects 0.000 claims 16
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 14
- 229920001184 polypeptide Polymers 0.000 claims 13
- 102000004196 processed proteins & peptides Human genes 0.000 claims 13
- 108090000765 processed proteins & peptides Proteins 0.000 claims 13
- 102000035195 Peptidases Human genes 0.000 claims 9
- 108010088535 Pep-1 peptide Proteins 0.000 claims 7
- 101150044010 SLP1 gene Proteins 0.000 claims 7
- 101150110969 gap1 gene Proteins 0.000 claims 7
- 101150055071 slaA gene Proteins 0.000 claims 7
- 101150060219 tsp-1 gene Proteins 0.000 claims 6
- 101100494726 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) pep-4 gene Proteins 0.000 claims 5
- 101150048694 gap2 gene Proteins 0.000 claims 5
- 102000040430 polynucleotide Human genes 0.000 claims 3
- 108091033319 polynucleotide Proteins 0.000 claims 3
- 239000002157 polynucleotide Substances 0.000 claims 3
- 101000599951 Homo sapiens Insulin-like growth factor I Proteins 0.000 claims 2
- 102000002265 Human Growth Hormone Human genes 0.000 claims 2
- 108010000521 Human Growth Hormone Proteins 0.000 claims 2
- 239000000854 Human Growth Hormone Substances 0.000 claims 2
- 102100037852 Insulin-like growth factor I Human genes 0.000 claims 2
- 230000003197 catalytic effect Effects 0.000 claims 2
- 238000012258 culturing Methods 0.000 claims 2
- SRCAXTIBNLIRHU-JJKPAIEPSA-N lantibiotic pep5 Chemical compound N([C@@H](C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@H](CS)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N\C(=C/C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N\C(=C/C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N\C(=C(/C)S)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CS)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(N)=O)C(=O)NCC(=O)N[C@@H](CS)C(=O)N[C@@H](CCCCN)C(O)=O)C(=O)[C@@H]1CCCN1C(=O)CNC(=O)[C@H](C)NC(=O)C(=O)CC SRCAXTIBNLIRHU-JJKPAIEPSA-N 0.000 claims 2
- 238000000034 method Methods 0.000 claims 2
- 230000035772 mutation Effects 0.000 claims 2
- -1 pep3 Proteins 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- 101100189913 Caenorhabditis elegans pept-1 gene Proteins 0.000 claims 1
- 108090000695 Cytokines Proteins 0.000 claims 1
- 102000004127 Cytokines Human genes 0.000 claims 1
- 102100039059 Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase Human genes 0.000 claims 1
- 102100021771 Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase Human genes 0.000 claims 1
- 108060003306 Galactosyltransferase Proteins 0.000 claims 1
- 102000030902 Galactosyltransferase Human genes 0.000 claims 1
- 101000958975 Homo sapiens Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase Proteins 0.000 claims 1
- 102100040018 Interferon alpha-2 Human genes 0.000 claims 1
- 108010079944 Interferon-alpha2b Proteins 0.000 claims 1
- 108010050904 Interferons Proteins 0.000 claims 1
- 102000014150 Interferons Human genes 0.000 claims 1
- 102000015696 Interleukins Human genes 0.000 claims 1
- 108010063738 Interleukins Proteins 0.000 claims 1
- 101100428706 Schizosaccharomyces pombe (strain 972 / ATCC 24843) vps26 gene Proteins 0.000 claims 1
- 101800000936 Structural peptide 4 Proteins 0.000 claims 1
- 108010070113 alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I Proteins 0.000 claims 1
- 239000000427 antigen Substances 0.000 claims 1
- 108091007433 antigens Proteins 0.000 claims 1
- 102000036639 antigens Human genes 0.000 claims 1
- 108010073219 beta-1,3-galactosyl-0-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase Proteins 0.000 claims 1
- 239000012634 fragment Substances 0.000 claims 1
- 230000002538 fungal effect Effects 0.000 claims 1
- 239000003102 growth factor Substances 0.000 claims 1
- 229940079322 interferon Drugs 0.000 claims 1
- 108010037248 lantibiotic Pep5 Proteins 0.000 claims 1
- 108010009689 mannosyl-oligosaccharide 1,2-alpha-mannosidase Proteins 0.000 claims 1
- 108010083819 mannosyl-oligosaccharide 1,3 - 1,6-alpha-mannosidase Proteins 0.000 claims 1
- 101150099695 vps11 gene Proteins 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261583559P | 2012-01-05 | 2012-01-05 | |
| US61/583,559 | 2012-01-05 | ||
| PCT/EP2013/050126 WO2013102674A2 (en) | 2012-01-05 | 2013-01-04 | Protease deficient filamentous fungal cells and methods of use thereof |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017212468A Division JP2018038419A (ja) | 2012-01-05 | 2017-11-02 | プロテアーゼ欠損糸状菌細胞及びその使用方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015512611A JP2015512611A (ja) | 2015-04-30 |
| JP2015512611A5 true JP2015512611A5 (enExample) | 2016-02-04 |
| JP6245528B2 JP6245528B2 (ja) | 2017-12-13 |
Family
ID=47520118
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014550709A Expired - Fee Related JP6245528B2 (ja) | 2012-01-05 | 2013-01-04 | プロテアーゼ欠損糸状菌細胞及びその使用方法 |
| JP2017212468A Pending JP2018038419A (ja) | 2012-01-05 | 2017-11-02 | プロテアーゼ欠損糸状菌細胞及びその使用方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017212468A Pending JP2018038419A (ja) | 2012-01-05 | 2017-11-02 | プロテアーゼ欠損糸状菌細胞及びその使用方法 |
Country Status (14)
| Country | Link |
|---|---|
| US (5) | US9567596B2 (enExample) |
| EP (2) | EP2800809B1 (enExample) |
| JP (2) | JP6245528B2 (enExample) |
| KR (1) | KR20140114860A (enExample) |
| CN (2) | CN107868758A (enExample) |
| AU (1) | AU2013207165B2 (enExample) |
| BR (1) | BR112014016587A2 (enExample) |
| CA (1) | CA2861697C (enExample) |
| DK (1) | DK2800809T3 (enExample) |
| IL (1) | IL233052A0 (enExample) |
| MX (1) | MX355226B (enExample) |
| RU (1) | RU2645252C2 (enExample) |
| SG (1) | SG11201403818XA (enExample) |
| WO (1) | WO2013102674A2 (enExample) |
Families Citing this family (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112014016587A2 (pt) * | 2012-01-05 | 2020-10-27 | Novartis Ag | células fúngicas filamentosas deficientes de protease e métodos de uso das mesmas |
| CA2906671A1 (en) * | 2013-03-14 | 2014-09-25 | Glycobia, Inc. | Oligosaccharide compositions, glycoproteins and methods to produce the same in prokaryotes |
| DK3019602T3 (en) | 2013-07-10 | 2018-11-12 | Glykos Finland Oy | MULTIPLE PROTEASE-DEFECTED FILAMENTARY FUNGAL CELLS AND PROCEDURES FOR USE THEREOF |
| CA2954974A1 (en) * | 2014-07-21 | 2016-01-28 | Glykos Finland Oy | Production of glycoproteins with mammalian-like n-glycans in filamentous fungi |
| CN107105709B (zh) * | 2014-10-24 | 2021-04-30 | 杜邦营养生物科学有限公司 | 脯氨酸耐受性三肽基肽酶在饲料添加剂组合物中的用途 |
| EP3172329A1 (en) * | 2014-10-24 | 2017-05-31 | Danisco US Inc. | Method for producing alcohol by use of a tripeptidyl peptidase |
| ES2815628T3 (es) | 2015-02-20 | 2021-03-30 | Roal Oy | Métodos para controlar la producción de proteasas |
| CN106190874B (zh) * | 2015-05-06 | 2020-06-26 | 中国科学院天津工业生物技术研究所 | 一种强化丝状真菌蛋白质生产的方法 |
| CN104990881A (zh) * | 2015-07-09 | 2015-10-21 | 辽宁省农业科学院蔬菜研究所 | 一种快速检测羊肚菌菌丝活力的方法 |
| CN108603187A (zh) * | 2015-11-24 | 2018-09-28 | 诺维信公司 | 具有蛋白酶活性的多肽以及编码它们的多核苷酸 |
| WO2017101060A1 (en) * | 2015-12-17 | 2017-06-22 | Evonik Degussa (China) Co., Ltd. | Gene cassette for homologous recombination knock-out in yeast cells |
| WO2017115005A1 (en) | 2015-12-29 | 2017-07-06 | Teknologian Tutkimuskeskus Vtt Oy | Production of fusion proteins in trichoderma |
| KR102593668B1 (ko) | 2016-10-04 | 2023-10-24 | 다니스코 유에스 인크. | 유도 기질 부재 하의 사상 진균 세포에서의 단백질 생산 |
| CN108588060B (zh) | 2017-03-07 | 2020-12-01 | 武汉康复得生物科技股份有限公司 | 一种用丝状真菌宿主细胞表达的重组草酸脱羧酶 |
| US11385238B2 (en) * | 2017-06-30 | 2022-07-12 | Amgen Inc. | Methods of protein clips recovery |
| US11795487B2 (en) | 2017-07-31 | 2023-10-24 | Melt & Marble AB | Fungal cell with improved protein production capacity |
| BR112020003866A2 (pt) | 2017-08-30 | 2020-09-08 | Novozymes A/S | processo para produção de um produto de fermentação, e, composição. |
| EP3696274A4 (en) | 2017-10-10 | 2021-07-28 | Ajinomoto Co., Inc. | PROTEIN MANUFACTURING PROCESS |
| WO2019175477A1 (en) | 2018-03-12 | 2019-09-19 | Teknologian Tutkimuskeskus Vtt Oy | A subunit vaccine against porcine post-weaning diarrhoea |
| US10787656B2 (en) * | 2018-05-04 | 2020-09-29 | Fomia BioSolutions, Inc. | Variant alkaline protease enzyme compositions and methods |
| BR112021003459A2 (pt) * | 2018-08-29 | 2021-05-11 | Toray Industries, Inc. | cepa mutante de trichoderma reesei e métodos para produzir uma proteína, para produzir uma celulase e para produzir um açúcar |
| CN111088244B (zh) * | 2018-10-23 | 2023-03-21 | 中国科学院天津工业生物技术研究所 | 蛋白酶基因在促进纤维素酶生产和复杂氮源利用中的应用 |
| US20220259270A1 (en) * | 2019-07-16 | 2022-08-18 | Centro Nacional De Pesquisa Em Energia E Materiais | Modified trichoderma fungal strain for the production of an enzyme cocktail |
| CN110724644A (zh) * | 2019-12-05 | 2020-01-24 | 西北农林科技大学 | 一种产重组人胰岛素原的里氏木霉工程菌及其应用 |
| CN115836127A (zh) * | 2020-06-11 | 2023-03-21 | 味之素株式会社 | 蛋白的制造方法 |
| EP4189061A1 (en) | 2020-07-31 | 2023-06-07 | Biotalys NV | Methods of increasing recombinant protein yields |
| CN113388532B (zh) * | 2021-06-16 | 2023-04-07 | 华东理工大学 | 一种用于生产天冬酰胺酶的重组里氏木霉及其构建方法和应用 |
| WO2023003683A2 (en) | 2021-07-19 | 2023-01-26 | Danisco Us Inc. | Compositions and methods for enhanced protein production in fungal cells |
| US20250236858A1 (en) | 2021-10-15 | 2025-07-24 | Danisco Us Inc. | Glucoamylase variants and methods for use thereof |
| CN118574921A (zh) | 2021-12-01 | 2024-08-30 | 丹尼斯科美国公司 | 用于增强真菌细胞中的蛋白质产生的组合物和方法 |
| EP4473119A1 (en) | 2022-02-02 | 2024-12-11 | Biotalys NV | Modified microbial cells and uses thereof |
| CN114907992B (zh) * | 2022-05-18 | 2023-06-16 | 塔里木大学 | 拮抗植物病原菌的菌株c11及其应用 |
| CN115820692B (zh) * | 2022-08-17 | 2024-05-10 | 大连理工大学 | 一种能同时达到去除羊毛虱卵壳与防毡缩的蛋白酶与应用 |
| AU2024271436A1 (en) * | 2023-05-15 | 2025-11-13 | Sangamo Therapeutics, Inc. | Engineered blood brain barrier penetrant aav capsids |
| WO2025005761A1 (ko) * | 2023-06-29 | 2025-01-02 | 씨제이제일제당 (주) | 아스파르트산 프로테아제 변이체 |
| WO2025230415A1 (en) | 2024-04-30 | 2025-11-06 | Ginkgo Bioworks Netherlands B.V. | Host cells expressing optb protein variants and uses thereof |
Family Cites Families (91)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4500707A (en) | 1980-02-29 | 1985-02-19 | University Patents, Inc. | Nucleosides useful in the preparation of polynucleotides |
| US4475196A (en) | 1981-03-06 | 1984-10-02 | Zor Clair G | Instrument for locating faults in aircraft passenger reading light and attendant call control system |
| US4447233A (en) | 1981-04-10 | 1984-05-08 | Parker-Hannifin Corporation | Medication infusion pump |
| US4439196A (en) | 1982-03-18 | 1984-03-27 | Merck & Co., Inc. | Osmotic drug delivery system |
| US4447224A (en) | 1982-09-20 | 1984-05-08 | Infusaid Corporation | Variable flow implantable infusion apparatus |
| US4487603A (en) | 1982-11-26 | 1984-12-11 | Cordis Corporation | Implantable microinfusion pump system |
| US4816567A (en) | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
| US4486194A (en) | 1983-06-08 | 1984-12-04 | James Ferrara | Therapeutic device for administering medicaments through the skin |
| US4758512A (en) | 1984-03-06 | 1988-07-19 | President And Fellows Of Harvard College | Hosts and methods for producing recombinant products in high yields |
| US4596556A (en) | 1985-03-25 | 1986-06-24 | Bioject, Inc. | Hypodermic injection apparatus |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4790824A (en) | 1987-06-19 | 1988-12-13 | Bioject, Inc. | Non-invasive hypodermic injection device |
| US4941880A (en) | 1987-06-19 | 1990-07-17 | Bioject, Inc. | Pre-filled ampule and non-invasive hypodermic injection device assembly |
| US5700637A (en) | 1988-05-03 | 1997-12-23 | Isis Innovation Limited | Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays |
| CA1333777C (en) | 1988-07-01 | 1995-01-03 | Randy M. Berka | Aspartic proteinase deficient filamentous fungi |
| GB8822228D0 (en) | 1988-09-21 | 1988-10-26 | Southern E M | Support-bound oligonucleotides |
| US5064413A (en) | 1989-11-09 | 1991-11-12 | Bioject, Inc. | Needleless hypodermic injection device |
| US5312335A (en) | 1989-11-09 | 1994-05-17 | Bioject Inc. | Needleless hypodermic injection device |
| GB2276169A (en) | 1990-07-05 | 1994-09-21 | Celltech Ltd | Antibodies specific for carcinoembryonic antigen |
| US5846802A (en) | 1992-04-15 | 1998-12-08 | Buxton; Frank | Fungal Protease |
| CA2096289A1 (en) | 1992-05-15 | 1993-11-16 | Wouter Musters | Cloning and expression of dna encoding a ripening form of a polypeptide having rhamnogalacturonase activity |
| US5383851A (en) | 1992-07-24 | 1995-01-24 | Bioject Inc. | Needleless hypodermic injection device |
| DK52393D0 (enExample) | 1993-05-05 | 1993-05-05 | Novo Nordisk As | |
| US5674728A (en) | 1993-11-03 | 1997-10-07 | Novartis Corporation | Aspergillus niger vacuolar aspartyl protease |
| ATE256191T1 (de) | 1994-06-17 | 2003-12-15 | Novozymes As | Ein pilz, in dem das area-gen verändert ist, und ein area-gen aus aspergillus oryzae |
| US6060305A (en) | 1994-06-30 | 2000-05-09 | Novo Nordisk Biotech, Inc. | Non-toxic, non-toxigenic, non-pathogenic Fusarium expression system |
| JPH10508475A (ja) | 1994-11-08 | 1998-08-25 | ノボ ノルディスク アクティーゼルスカブ | トリペプチジルアミノペプチダーゼ |
| ATE353953T1 (de) | 1995-03-20 | 2007-03-15 | Novozymes As | Wirtszelle, die reduzierte mengen einer metalloprotease exprimiert und methoden zur verwendung dieser wirtszelle zur proteinproduktion |
| AU7112796A (en) | 1995-09-27 | 1997-04-17 | Chiron Corporation | Method of making a protease deficient host |
| AU1092897A (en) | 1995-12-15 | 1997-07-14 | Novo Nordisk A/S | A funguns wherein the area, pepc and/or pepe genes have been inactivated |
| US5776730A (en) | 1995-12-15 | 1998-07-07 | University Of Hawaii | Neurospora hosts for the production of recombinant proteins, and methods for producing same |
| EP0877801B1 (en) | 1996-01-19 | 2005-04-27 | Novozymes Biotech, Inc. | Morphological mutants of filamentous fungi |
| JP4263241B2 (ja) | 1996-03-27 | 2009-05-13 | ノボザイムス アクティーゼルスカブ | アルカリプロテアーゼを欠失した糸状真菌 |
| EP0907744A1 (en) | 1996-06-05 | 1999-04-14 | Gist-Brocades B.V. | Fungal metallo protease genes |
| ATE313620T1 (de) | 1996-09-19 | 2006-01-15 | Novozymes As | Wirtszellen und methoden für die produktion von proteinen |
| US7883872B2 (en) | 1996-10-10 | 2011-02-08 | Dyadic International (Usa), Inc. | Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose |
| US6806062B1 (en) | 1998-10-05 | 2004-10-19 | Novozymes A/S | Fungal transcriptional activator useful in methods for producing polypeptides |
| JP2002535990A (ja) * | 1999-02-02 | 2002-10-29 | ノボザイムス バイオテック，インコーポレイティド | 真菌細胞においてポリペプチドを生産する方法 |
| CN1195058C (zh) * | 1999-02-22 | 2005-03-30 | 诺维信公司 | 草酰乙酸水解酶缺陷型真菌宿主细胞 |
| ATE469223T1 (de) | 2000-03-14 | 2010-06-15 | Novozymes As | Pilz transkriptionsaktivator zur verwendung in verfahren zur herstellung von polypeptiden |
| MXPA01012905A (es) * | 2000-04-13 | 2002-11-22 | Mark Aaron Emalfarb | Clasificacion de alto rendimiento de bibliotecas de adn expresado en hongo filamentos. |
| KR20020026456A (ko) | 2000-04-13 | 2002-04-10 | 에말파브 마크 아론 | 사상균에서 발현된 ｄｎａ 라이브러리의 고산출량 스크리닝 |
| WO2002046381A2 (en) | 2000-12-07 | 2002-06-13 | Dsm N.V. | Method for the prevention or reduction of haze in beverages |
| AU2002308306B2 (en) | 2001-02-23 | 2007-03-01 | Dsm Ip Assets B.V. | Genes encoding proteolytic enzymes from aspargilli |
| WO2003054186A1 (en) | 2001-12-21 | 2003-07-03 | Dsm Ip Assets B.V. | New rennets |
| EP1495055B1 (en) | 2002-04-18 | 2013-08-14 | Genencor International, Inc. | Production of functional antibodies in filamentous fungi |
| WO2004067709A2 (en) | 2003-01-17 | 2004-08-12 | Elitra Pharmaceuticals, Inc. | Identification of essential genes of aspergillus fumigatus and methods of use |
| EP1613761B1 (en) | 2003-03-31 | 2009-09-02 | Novozymes Inc. | Methods for producing biological substances in enzyme-deficient mutants of aspergillus niger |
| JP4842834B2 (ja) | 2003-12-09 | 2011-12-21 | ノボザイムス，インコーポレイティド | 糸状菌株における遺伝子の発現を排除するか又は低めるための方法 |
| GB0405659D0 (en) | 2004-03-12 | 2004-04-21 | Horticulture Res Internat | Altered expression in filamentous fungi |
| US7968312B2 (en) | 2004-06-16 | 2011-06-28 | Dsm Ip Assets B.V. | Production of polypeptides by improved secretion |
| CN101094919B (zh) | 2004-10-12 | 2014-09-03 | 帝斯曼知识产权资产管理有限公司 | 在生产多肽的方法中有用的真菌转录活化因子 |
| GB0424940D0 (en) | 2004-11-11 | 2004-12-15 | Danisco | Transcription factors |
| DK1831362T3 (da) | 2004-12-30 | 2012-01-09 | Genencor Int | Syreaktive svampeproteaser |
| DK2333045T3 (en) | 2005-04-12 | 2017-02-20 | Danisco Us Inc | GEN-INACTIVATED MUTANTS WITH CHANGED PROTEIN PRODUCTION |
| EP2182060B1 (en) | 2005-08-03 | 2012-10-24 | Asahi Glass Company, Limited | Transformed yeast host cells and method of producing foreign protein |
| EP1941025B1 (en) * | 2005-10-17 | 2011-06-29 | Novozymes A/S | Use of fungal mutants for expression of antibodies |
| WO2007062936A2 (en) | 2005-11-29 | 2007-06-07 | Dsm Ip Assets B.V. | Dna binding site of a transcriptional activator useful in gene expression |
| EP2423315B1 (en) | 2006-06-29 | 2015-01-07 | DSM IP Assets B.V. | A method for achieving improved polypeptide expression |
| BRPI0716219A2 (pt) | 2006-08-29 | 2013-10-15 | Danisco Us Inc Genencor Div | Composições e métodos para produção aperfeiçoada de proteína. |
| US8389269B2 (en) | 2006-11-02 | 2013-03-05 | Dsm Ip Assets B.V. | Production of secreted proteins by filamentous fungi |
| US8680252B2 (en) | 2006-12-10 | 2014-03-25 | Dyadic International (Usa), Inc. | Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi |
| US9862956B2 (en) * | 2006-12-10 | 2018-01-09 | Danisco Us Inc. | Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi |
| CN101652479A (zh) | 2006-12-21 | 2010-02-17 | 诺维信股份有限公司 | 通过移行rna干扰来降低或消除丝状真菌菌株中的基因表达的方法 |
| EP2508613A3 (en) * | 2007-04-03 | 2012-11-28 | Oxyrane UK Limited | Glycosylation of molecules |
| WO2008153712A2 (en) | 2007-05-21 | 2008-12-18 | Danisco Us, Inc., Genencor Division | Method for introducing nucleic acids into fungal cells |
| CA2704548A1 (en) * | 2007-11-01 | 2009-05-07 | Danisco Us Inc. | Signal sequences and co-expressed chaperones for improving protein production in a host cell |
| US8633010B2 (en) | 2007-12-06 | 2014-01-21 | Novozymes A/S | Fungal PepC inhibitor |
| US20090253173A1 (en) | 2008-04-08 | 2009-10-08 | Danisco Us Inc., Genencor Division | Filamentous fungi with inactivated protease genes for altered protein production |
| CA2729839A1 (en) | 2008-07-03 | 2010-01-07 | Diane Retallack | High throughput screening method and use thereof to identify a production platform for a multifunctional binding protein |
| CN102099466A (zh) | 2008-07-29 | 2011-06-15 | 丹尼斯科美国公司 | 在丝状真菌细胞中增加天冬氨酸蛋白酶的产生 |
| CN102227502B (zh) | 2008-09-30 | 2016-01-13 | 诺维信股份有限公司 | 在镶片镰孢的酶缺陷突变体中产生多肽的方法 |
| EP2350303A1 (en) * | 2008-11-18 | 2011-08-03 | Danisco US Inc. | Filamentous fungi with impaired ptrb activity for altered protein production |
| US9493759B2 (en) | 2008-12-12 | 2016-11-15 | Novozymes, Inc. | Polypeptides having aspartic endopeptidase activity and polynucleotides encoding same |
| CA2755417C (en) | 2009-03-16 | 2018-11-13 | Dyadic Nederland B.V. | New fungal production system |
| CN104031961A (zh) | 2009-10-16 | 2014-09-10 | 默沙东公司 | 生产成熟的人促红细胞生成素的方法以及包含获自该方法的人促红细胞生成素的组合物 |
| US20120213728A1 (en) | 2009-10-30 | 2012-08-23 | Merck | Granulocyte-colony stimulating factor produced in glycoengineered pichia pastoris |
| EP2494030A4 (en) | 2009-10-30 | 2013-03-27 | Merck Sharp & Dohme | METHOD FOR THE PRODUCTION OF THERAPEUTIC PROTEINS IN PICHIA PASTORIS WITHOUT DIPEPTIDYL-AMINOPEPTIDASE ACTIVITY |
| MX2012004993A (es) | 2009-10-30 | 2012-06-12 | Merck Sharp & Dohme | Metodos para la produccion de proteinas recombinantes con eficiencias de secrecion mejoradas. |
| BR112012011980A2 (pt) * | 2009-11-19 | 2021-09-08 | Oxyrane Uk Limited | Métodos de produzir células transformadas de yarrowia lipolytica e proteínas-alvocompreendendo n-glicanos, células transformadas de yarrowia lipolytica e suas culturas, bem como composição compreendendo glicoproteínas |
| BR112012013205B1 (pt) | 2009-12-18 | 2020-11-03 | Novozymes, Inc. | mutante de uma cepa parental de trichoderma reesei, e, métodos para produzir um polipeptídeo de interesse e para obter um mutante de uma cepa parental de trichoderma |
| AU2010334383B2 (en) | 2009-12-21 | 2014-10-02 | Centre Hospitalier Universitaire Vaudois (Chuv) | Synergic action of a prolyl protease and tripeptidyl proteases |
| CN102858949B (zh) | 2010-02-24 | 2016-07-06 | 默沙东公司 | 用于增加在巴氏毕赤酵母中生产的治疗性糖蛋白上的n-糖基化位点占据的方法 |
| WO2012021883A2 (en) | 2010-08-13 | 2012-02-16 | Dyadic International, Inc. | Novel fungal enzymes |
| WO2012024662A2 (en) | 2010-08-20 | 2012-02-23 | Codexis, Inc. | Expression constructs comprising fungal promoters |
| WO2012048334A2 (en) | 2010-10-08 | 2012-04-12 | Dyadic International (Usa) Inc. | Novel fungal proteases |
| PL2635671T3 (pl) | 2010-11-02 | 2017-02-28 | Codexis, Inc. | Ulepszone szczepy grzybowe |
| IN2014CN02136A (enExample) * | 2011-08-24 | 2015-05-29 | Novozymes Inc | |
| BR112014007719A2 (pt) * | 2011-09-30 | 2017-06-13 | Codexis Inc | proteases fúngicas |
| BR112014016587A2 (pt) * | 2012-01-05 | 2020-10-27 | Novartis Ag | células fúngicas filamentosas deficientes de protease e métodos de uso das mesmas |
| US20150337279A1 (en) | 2012-11-20 | 2015-11-26 | Codexis, Inc. | Recombinant fungal polypeptides |
-
2013
- 2013-01-04 BR BR112014016587-4A patent/BR112014016587A2/pt not_active Application Discontinuation
- 2013-01-04 KR KR1020147021955A patent/KR20140114860A/ko not_active Abandoned
- 2013-01-04 JP JP2014550709A patent/JP6245528B2/ja not_active Expired - Fee Related
- 2013-01-04 CN CN201710503139.1A patent/CN107868758A/zh active Pending
- 2013-01-04 CN CN201380012940.6A patent/CN104245919B/zh not_active Expired - Fee Related
- 2013-01-04 DK DK13700021.2T patent/DK2800809T3/en active
- 2013-01-04 AU AU2013207165A patent/AU2013207165B2/en not_active Ceased
- 2013-01-04 WO PCT/EP2013/050126 patent/WO2013102674A2/en not_active Ceased
- 2013-01-04 US US14/370,255 patent/US9567596B2/en active Active
- 2013-01-04 RU RU2014132178A patent/RU2645252C2/ru not_active IP Right Cessation
- 2013-01-04 MX MX2014008272A patent/MX355226B/es active IP Right Grant
- 2013-01-04 EP EP13700021.2A patent/EP2800809B1/en not_active Revoked
- 2013-01-04 CA CA2861697A patent/CA2861697C/en active Active
- 2013-01-04 EP EP17210254.3A patent/EP3358000A3/en not_active Withdrawn
- 2013-01-04 SG SG11201403818XA patent/SG11201403818XA/en unknown
-
2014
- 2014-06-10 IL IL233052A patent/IL233052A0/en not_active IP Right Cessation
-
2016
- 2016-12-12 US US15/375,635 patent/US10240159B2/en active Active
-
2017
- 2017-11-02 JP JP2017212468A patent/JP2018038419A/ja active Pending
-
2019
- 2019-01-18 US US16/251,194 patent/US10731168B2/en active Active
-
2020
- 2020-06-26 US US16/912,787 patent/US11180767B2/en active Active
-
2021
- 2021-11-18 US US17/529,931 patent/US11827891B2/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2015512611A5 (enExample) | ||
| RU2014132178A (ru) | Клетки нитчатых грибов с дефицитом протеаз и способы их применения | |
| RU2016104064A (ru) | Клетки мицелиальных грибов с множественной недостаточностью протеаз и способы их использования | |
| Peña et al. | Metabolic engineering of Pichia pastoris | |
| Vieira Gomes et al. | Comparison of yeasts as hosts for recombinant protein production | |
| Idiris et al. | Engineering of protein secretion in yeast: strategies and impact on protein production | |
| JP2009502167A5 (enExample) | ||
| JP2016512848A5 (enExample) | ||
| JP2012152218A5 (enExample) | ||
| JP2014507144A5 (ja) | 慢性ｂ型肝炎感染症のための酵母系免疫療法組成物 | |
| JP2013511272A5 (enExample) | ||
| ATE371035T1 (de) | Neue neomycin-phosphotransferase-gene und verfahren zur selektion von hochproduzierenden rekombinanten zellen | |
| JP2022043355A (ja) | 組換えヒト血清アルブミンの高発現のための遺伝子工学操作された細菌の構築 | |
| JP2014500019A5 (enExample) | ||
| Bae et al. | An efficient genome-wide fusion partner screening system for secretion of recombinant proteins in yeast | |
| JP2015503338A (ja) | 組換えタンパク質の分解を低減させる方法および材料 | |
| JP2016514957A5 (enExample) | ||
| Zahrl et al. | Detection and elimination of cellular bottlenecks in protein-producing yeasts | |
| MX2021015818A (es) | Proteinas de fusion para mejorar la expresion de enzimas. | |
| JP2014525247A5 (enExample) | ||
| ZA202203208B (en) | Recombinantly engineered, lipase/esterase-deficient mammalian cell lines | |
| JP2016531581A5 (enExample) | ||
| Sun et al. | High level expression and purification of active recombinant human interleukin-15 in Pichia pastoris | |
| CN118389529A (zh) | 一种适于在毕赤酵母中表达的人血清白蛋白优化基因及在毕赤酵母中表达的方法 | |
| Maity et al. | Expression and control of codon-optimized granulocyte colony-stimulating factor in Pichia pastoris |