CN107868758A - 蛋白酶缺陷丝状真菌细胞及其使用方法 - Google Patents

蛋白酶缺陷丝状真菌细胞及其使用方法 Download PDF

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CN107868758A
CN107868758A CN201710503139.1A CN201710503139A CN107868758A CN 107868758 A CN107868758 A CN 107868758A CN 201710503139 A CN201710503139 A CN 201710503139A CN 107868758 A CN107868758 A CN 107868758A
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C·兰道斯基
A·雨斯科南
J·萨里南
A·韦斯特霍尔姆-帕维南
A·卡内尔瓦
J·纳蒂南
A-L·阿尼南
N·萨洛维奥里
M·彭蒂拉
M·萨洛埃莫
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Abstract

本公开涉及可用于在丝状真菌细胞，特别是包含三种具有降低的活性的内源蛋白酶和编码异源多肽的重组多核苷酸的丝状真菌细胞，中产生异源蛋白的组合物和方法，其中所述多肽以比其中所述蛋白酶不具有降低的活性的对应亲本丝状真菌细胞中该多肽的产生水平高至少两倍的水平产生。

Description

蛋白酶缺陷丝状真菌细胞及其使用方法
本申请是申请日为2013年1月4日和发明名称为“蛋白酶缺陷丝状真菌 细胞及其使用方法”的201380012940.6号发明专利申请的分案申请。
发明领域
本公开涉及可用于在丝状真菌细胞中产生异源蛋白质的组合物和方法。
背景技术
真核生物蛋白质(特别是治疗性蛋白质如免疫球蛋白)的翻译后修饰通 常是正确的蛋白质折叠和功能必需的。因为标准的原核表达系统缺乏这类修 饰所需的适当机制，必须使用替代的表达系统来产生这些治疗性蛋白质。即 使在真核蛋白质不具有翻译后修饰的情况中，原核表达系统还通常缺乏正确 折叠所需的必要的伴侣蛋白。酵母和真菌对于表达蛋白质是有吸引力的选项， 因为它们可以容易地在简单培养基中大规模生长，这允许低的生产成本，且 酵母和真菌具有执行与在哺乳动物细胞中发现的相似功能的翻译后机制和伴 侣蛋白。此外，操作酵母和真菌细胞的相对简单的遗传构成以及更复杂的真 核细胞如哺乳动物或昆虫细胞的工具是可用的(De Pourcq等,Appl Microbiol Biotechnol,87(5):1617-31)。尽管存在这些优点，许多治疗性蛋白质仍然在哺 乳动物中产生，其产生具有与天然人蛋白质最相似的翻译后修饰的治疗性蛋 白质，而由酵母和真菌天然产生的翻译后修饰通常与哺乳动物中发现的不同。
为解决这种不足，开发了产生与天然人蛋白质中发现的翻译后修饰更相 似的翻译后修饰新的酵母和真菌菌株。因此，存在着使用酵母和真菌细胞表 达更复杂的蛋白质的新兴趣。但是，由于工业上长时间内集中于哺乳动物细 胞培养技术，真菌细胞表达系统如木霉属未如哺乳动物细胞培养一样良好建 立且因此在表达哺乳动物蛋白质时存在缺陷。
因此，在本领域中仍需要可以稳定地产生异源蛋白质如免疫球蛋白的改 进的丝状真菌细胞如木霉属真菌细胞，优选以高表达水平产生。
发明概述
本文描述的是包括具有降低的或没有可检测的至少三种蛋白酶的活性且 具有编码以提高的水平产生的异源多肽的重组多核苷酸的丝状真菌细胞如木 霉属真菌细胞的组合物。本文进一步描述的是提高异源多肽稳定性的方法和 制备其中蛋白酶不具有降低的活性的异源多肽的方法。
因此，一个方面包括具有降低的或没有可检测的至少三种蛋白酶的活性 的丝状真菌细胞，其中该细胞进一步包含编码异源多肽的重组多核苷酸，该 异源多肽以比其中所述蛋白酶不具有降低的活性的对应亲本丝状真菌细胞中 该多肽的产生水平高至少两倍的水平产生。在某些实施方式中，当所述细胞 是曲霉属细胞时，总蛋白酶活性降低到其中所述蛋白酶不具有降低的活性的 对应亲本曲霉属细胞的总蛋白酶活性的50％或更低。在其它实施方式中，丝 状真菌细胞的总蛋白酶活性降低到其中所述蛋白酶不具有降低的活性的对应 亲本丝状真菌细胞的总蛋白酶活性的49％或更低，31％或更低。
在某些实施方式中，至少三种蛋白酶的表达水平被降低或消除。在某些 实施方式中，编码三种蛋白酶的基因各自包含降低或消除相应蛋白酶活性的 突变。在可以与前述实施方式组合的某些实施方式中，三种蛋白酶编码基因 是pep1、tsp1和slp1。在其它实施方式中，三种蛋白酶编码基因是gap1、slp1 和pep1。
在某些实施方式中，真菌细胞的四种内源蛋白酶具有降低的或没有可检 测的活性；编码该四种蛋白酶的基因各自包含降低或消除相应蛋白酶活性的 突变。在可以与前述实施方式组合的某些实施方式中，四种蛋白酶编码基因 是pep1、tsp1、slp1和gap1。
在可以与前述实施方式组合的某些实施方式中，三种或四种蛋白酶编码 基因选自pep1、pep2、pep3、pep4、pep5、pep8、pep11、pep12、tsp1、slp1、 slp2、slp3、slp7、gap1和gap2。在可以与前述实施方式组合的某些实施方式 中，三种或四种蛋白酶编码基因选自pep1、pep3、pep4、tsp1、slp1、slp2、 gap1和gap2。在某些实施方式中，三种或四种蛋白酶编码基因选自pep1、 pep2、pep3、pep4、pep5、gap1、gap2、slp1、slp2和tsp1。
在某些实施方式中，真菌细胞的五种内源蛋白酶具有降低的或没有可检 测的活性；编码该五种蛋白酶的基因各自包含降低或消除相应蛋白酶活性的 突变。在可以与前述实施方式组合的某些实施方式中，五种蛋白酶编码基因 是pep1、tsp1、slp1、gap1和pep4。在其它实施方式中，五种蛋白酶编码基 因是pep1、tsp1、slp1、gap1和gap2。
在某些实施方式中，真菌细胞的六种内源蛋白酶具有降低的或没有可检 测的活性；编码该六种蛋白酶的基因各自包含降低或消除相应蛋白酶活性的 突变。在某些实施方式中，所述细胞具有六种蛋白酶编码基因，其各包含降 低或消除相应蛋白酶活性的突变，且六种蛋白酶编码基因是pep1、tsp1、slp1、 gap1、gap2和pep4。
在可以与前述实施方式组合的某些实施方式中，真菌细胞具有三到六种 具有降低的或没有可检测的活性的蛋白酶，该三到六种蛋白酶各选自pep1、 pep2、pep3、pep4、pep5、tsp1、slp1、slp2、slp3、gap1和gap2。
在可以与前述实施方式组合的某些实施方式中，细胞具有七种蛋白酶编 码基因，其各包含降低或消除相应蛋白酶活性的突变，且七种蛋白酶编码基 因是pep1、tsp1、slp1、gap1、gap2、pep4和pep3。
在可以与前述实施方式组合的某些实施方式中，细胞具有八种蛋白酶编 码基因，其各包含降低或消除相应蛋白酶活性的突变，且八种蛋白酶编码基 因是pep1、tsp1、slp1、gap1、gap2、pep4、pep3和pep5。
在可以与前述实施方式组合的某些实施方式中，真菌细胞具有活性降低 的另外的蛋白酶，编码另外的蛋白酶的基因包含降低或消除相应蛋白酶活性 的突变，且另外的蛋白酶选自pep7、pep8、pep11、pep12、tpp1、gap2、slp3、 slp5、slp6、slp7和slp8。
在可以与前述实施方式组合的某些实施方式中，异源多肽是哺乳动物多 肽。在某些实施方式中，哺乳动物多肽是糖基化的。
在某些实施方式中，哺乳动物多肽选自免疫球蛋白、抗体及其抗原结合 片段、生长因子、干扰素、细胞因子和白介素。在某些实施方式中，哺乳动 物多肽是免疫球蛋白或抗体。在某些实施方式中，哺乳动物多肽选自胰岛素 样生长因子1(IGF1)、人生长激素(hGH)和干扰素α2b(IFNα2b)。
在可以与前述实施方式组合的某些实施方式中，异源多肽是非哺乳动物 多肽。在某些实施方式中，非哺乳动物多肽是氨肽酶、淀粉酶、糖酶、羧肽 酶、过氧化氢酶、纤维素酶、壳多糖酶、角质酶、脱氧核糖核酸酶、酯酶、 α-半乳糖苷酶、β-半乳糖苷酶、葡糖淀粉酶、α-葡糖苷酶、β-葡糖苷酶、转化 酶、漆酶、脂肪酶、聚糖酶、氧化酶、果胶分解酶(pectinolytic enzyme)、过 氧化物酶、磷脂酶、肌醇六磷酸酶、多酚氧化酶、蛋白水解酶、核糖核酸酶、 转谷氨酰胺酶或木聚糖酶。
在可以与前述实施方式组合的某些实施方式中，真菌细胞进一步包含 ALG3(甘露糖基转移酶)的降低的活性或没有可检测的活性。在某些实施方 式中，编码ALG3的基因包含降低或消除相应活性的突变。在可以与前述实 施方式组合的某些实施方式中，真菌细胞进一步包含编码α-1,2-甘露糖苷酶 的多核苷酸。
在可以与前述实施方式组合的某些实施方式中，真菌细胞具有降低希望 活性降低的蛋白酶的表达的突变。在可以与前述实施方式组合的某些实施方 式中，突变是编码蛋白酶的基因内的缺失。在可以与前述实施方式组合的某 些实施方式中，突变是编码蛋白酶的催化结构域的基因部分的缺失。在可以 与前述实施方式组合的某些实施方式中，真菌细胞具有编码蛋白酶的催化结 构域的基因部分中的点突变。
在其它实施方式中，一种或多种蛋白酶的蛋白酶活性的降低或消除由对 于选自pep-型蛋白酶、胰蛋白酶样丝氨酸蛋白酶、gap-型蛋白酶、sedolisin 蛋白酶和slp-型蛋白酶的i)一种蛋白酶或ii)两种或更多种蛋白酶特异性的 RNAi构建体产生。在某些实施方式中，RNAi构建体对于slp2、slp3、slp5 和/或slp6是特异性的。
在可以与前述实施方式组合的某些实施方式中，真菌细胞进一步包含N- 乙酰葡糖胺基转移酶I催化结构域和N-乙酰葡糖胺基转移酶II催化结构域。 在某些实施方式中，N-乙酰葡糖胺基转移酶I催化结构域和N-乙酰葡糖胺基 转移酶II催化结构域由多核苷酸编码。在某些实施方式中，N-乙酰葡糖胺基 转移酶I催化结构域由第一多核苷酸编码和N-乙酰葡糖胺基转移酶II催化结 构域由第二多核苷酸编码。在可以与前述实施方式组合的某些实施方式中， 真菌细胞进一步包含编码甘露糖苷酶II和/或半乳糖基转移酶的多核苷酸。在 某些实施方式中，真菌细胞包含选自α1,2-甘露糖苷酶、N-乙酰葡糖胺基转移 酶I、N-乙酰葡糖胺基转移酶II、甘露糖苷酶II和/或半乳糖基转移酶的酶， 所述酶进一步包括靶向肽，例如，用于相应酶的正确定位的异源靶向肽。在 某些实施方式中，靶向肽选自SEQID NO:589-594。
在可以与前述实施方式组合的某些实施方式中，真菌细胞是木霉属真菌 细胞、毁丝霉属真菌细胞、曲霉属真菌细胞、链孢霉属真菌细胞、镰孢菌属 或青霉菌属真菌细胞或者金孢子菌属真菌细胞。在可以与前述实施方式组合 的某些实施方式中，真菌细胞是里氏木霉(Trichoderma reesei)。
在可以与前述实施方式组合的某些实施方式中，真菌细胞对于pep4蛋白 酶是野生型的。
另一个方面包括通过以下方式提高异源多肽稳定性的方法：a)提供前述 任何实施方式的丝状真菌细胞和b)培养该细胞以表达该异源多肽，其中所述 异源多肽与其中所述蛋白酶不具有降低的活性(例如，如不包含编码该蛋白 酶的基因的突变)的对应亲本丝状真菌细胞中产生的异源多肽相比具有提高 的稳定性。另一个方面包括通过以下方式制备异源多肽的方法：a)提供前述 任何实施方式的丝状真菌细胞；b)培养所述宿主细胞以使得表达所述异源多 肽，和c)纯化所述异源多肽。在可以与前述实施方式组合的某些实施方式中， 所述丝状真菌细胞进一步包含载体蛋白。在某些实施方式中，所述载体蛋白 是CHB1。在可以与前述实施方式组合的某些实施方式中，所述培养是在包 含蛋白酶抑制剂的培养基中。在某些实施方式中，所述培养是在具有选自 SBT1和胰凝乳蛋白酶抑制剂的一种或两种蛋白酶抑制剂的培养基中。在某些 实施方式中，根据该方法产生的异源多肽是糖基化的哺乳动物多肽且多肽的 总N-聚糖的至少10％、至少20％、至少30％、至少40％、至少50％、至少 60％、至少70％、至少80％、至少90％或100％(摩尔％)由Man3GlcNAc2N- 聚糖组成。在其它实施方式中，根据该方法产生的异源多肽是糖基化的哺乳 动物多肽且多肽的总N-聚糖的至少10％、至少20％、至少30％、至少40％、 至少50％、至少60％、至少70％、至少80％、至少90％或100％(摩尔％)由 复合N-聚糖组成。在某些实施方式中，根据该方法产生的异源多肽是糖基化 的哺乳动物多肽且多肽的总N-聚糖的至少10％、至少20％、至少30％、至少 40％、至少50％、至少60％、至少70％、至少80％、至少90％或100％(摩尔 ％)由杂合N-聚糖组成。在某些实施方式中，根据该方法产生的异源多肽是糖 基化的哺乳动物多肽且多肽的总N-聚糖的至少10％、至少20％、至少30％、 至少40％、至少50％、至少60％、至少70％、至少80％、至少90％或100％(摩 尔％)由G1或G2N-聚糖组成。另一个方面包括可通过上述方法获得的异源 多肽。
另一个方面包括选自pep1、pep2、pep3、pep4、pep5、tsp1、slp1、slp2、 gap1和gap2的至少三种蛋白酶具有降低的或没有可检测的活性的木霉属真 菌细胞，其中所述细胞进一步包含编码产生水平比对应亲本木霉属真菌细胞 中该多肽的产生水平高至少2倍的哺乳动物多肽的重组多核苷酸。
在某些实施方式中，至少三种蛋白酶的表达水平在木霉属真菌细胞中降 低或消除。在某些实施方式中，编码至少三种蛋白酶的基因各自包含降低或 消除木霉属真菌细胞中相应蛋白酶活性的突变。在某些实施方式中，木霉属 真菌细胞包括具有降低或消除蛋白酶活性的突变的三个蛋白酶编码基因，其 选自gap1、slp1和pep1。在可以与前述实施方式组合的某些实施方式中，木 霉属真菌细胞中的哺乳动物多肽是抗体或其抗原结合片段或者免疫球蛋白， 且该至少三种蛋白酶选自pep1、pep3、pep4、tsp1、slp1、slp2、gap1和gap2。在某些实施方式中，木霉属真菌细胞包含四种蛋白酶编码基因，其各包含降 低或消除相应蛋白酶活性的突变，且具有这种突变的四种蛋白酶编码基因是 pep1、tsp1、slp1和gap1。在某些实施方式中，木霉属真菌细胞具有五种蛋 白酶编码基因，其各包含降低或消除相应蛋白酶活性的突变，且具有这种突 变的五种蛋白酶编码基因是pep1、tsp1、slp1、gap1和pep4。在可以与前述 实施方式组合的某些实施方式中，木霉属真菌细胞中的哺乳动物多肽是生长 因子、干扰素、细胞因子或白介素，且具有降低的活性的三种蛋白酶选自pep1、pep2、pep3、pep4、pep5、pep8、pep11、pep12、gap1、gap2、slp1、slp2、 slp1和tsp1。在某些实施方式中，木霉属真菌细胞具有五种蛋白酶编码基因， 其各包含降低或消除相应蛋白酶活性的突变，且具有这种突变的五种蛋白酶 编码基因是pep1、tsp1、slp1、gap1和gap2。在某些实施方式中，木霉属真 菌细胞具有六种蛋白酶编码基因，其各包含降低或消除相应蛋白酶活性的突 变，且具有这种突变的六种蛋白酶编码基因是pep1、tsp1、slp1、gap1、gap2 和pep4。在可以与前述实施方式组合的某些实施方式中，木霉属真菌细胞具 有七种蛋白酶编码基因，其各包含降低或消除相应蛋白酶活性的突变，且七 种蛋白酶编码基因是pep1、tsp1、slp1、gap1、gap2、pep4和pep3。在可以 与前述实施方式组合的某些实施方式中，木霉属真菌细胞具有八种蛋白酶编 码基因，其各包含降低相应蛋白酶活性的突变，且具有这种突变的八种蛋白 酶编码基因是pep1、tsp1、slp1、gap1、gap2、pep4、pep3和pep5。
在可以与前述实施方式组合的某些实施方式中，木霉属真菌细胞进一步 包含一种或多种另外的蛋白酶的降低的活性或没有可检测的活性。在某些实 施方式中，降低或消除木霉属真菌细胞中一种或多种另外的蛋白酶的表达水 平。在某些实施方式中，木霉属真菌细胞中编码一种或多种另外的蛋白酶的 基因各具有降低或消除相应蛋白酶活性的突变。在可以与前述实施方式组合 的某些实施方式中，一种或多种另外的蛋白酶编码基因选自pep7、pep8、 pep11、pep12、tpp1、gap2、slp3、slp5、slp6、slp7和slp8。
在可以与前述实施方式组合的某些实施方式中，木霉属真菌细胞进一步 包含降低的或没有可检测的ALG3活性。在某些实施方式中，木霉属真菌细 胞中编码ALG3的基因包含降低或消除相应活性的突变。在可以与前述实施 方式组合的某些实施方式中，木霉属真菌细胞进一步包含编码α-1,2-甘露糖 苷酶的多核苷酸。在可以与前述实施方式组合的某些实施方式中，突变降低 或消除木霉属真菌细胞中基因的表达。在可以与前述实施方式组合的某些实 施方式中，突变是木霉属真菌细胞中基因的缺失。在可以与前述实施方式组合的某些实施方式中，突变是木霉属真菌细胞中编码蛋白酶的催化结构域的 基因部分的缺失。在可以与前述实施方式组合的某些实施方式中，突变是木 霉属真菌细胞中编码蛋白酶的催化结构域的基因部分中的点突变。在可以与 前述实施方式组合的某些实施方式中，木霉属真菌细胞进一步包含N-乙酰葡 糖胺基转移酶I催化结构域和N-乙酰葡糖胺基转移酶II催化结构域。在某些 实施方式中，N-乙酰葡糖胺基转移酶I催化结构域和N-乙酰葡糖胺基转移酶 II催化结构域通过木霉属真菌细胞的多核苷酸编码。在某些实施方式中，N-乙酰葡糖胺基转移酶I催化结构域通过木霉属真菌细胞的第一多核苷酸编码 和N-乙酰葡糖胺基转移酶II催化结构域通过木霉属真菌细胞的第二多核苷酸 编码。在可以与前述实施方式组合的某些实施方式中，木霉属真菌细胞进一 步包含编码甘露糖苷酶II的多核苷酸。在某些实施方式中，蛋白质各与选自 SEQ ID NO:1、17、37、58、66、82、98、118、129、166和182的氨基酸序 列具有至少70％、至少75％、至少80％、至少85％、至少90％、至少95％、至少96％、至少97％、至少98％、至少99％或100％的序列同一性。在某些 实施方式中，木霉属真菌细胞中的总蛋白酶活性降低到其中所述蛋白酶不具 有降低的活性的对应木霉属亲本细胞的总蛋白酶活性的49％或更低，31％或 更低。在可以与前述实施方式组合的某些实施方式中，细胞进一步包含编码 产生水平比对应亲本木霉属真菌细胞中多肽的产生水平高至少两倍的的哺乳 动物多肽的重组多核苷酸。在可以与前述实施方式组合的某些实施方式中， 哺乳动物多肽以全长形式产生，其水平高于对应亲本木霉属真菌细胞中全长 形式多肽的产生水平。
另一个方面包括通过以下方式提高异源多肽稳定性的方法：a)提供前述 任一实施方式的木霉属真菌细胞；和b)培养所述细胞以表达异源多肽，其中 该异源多肽与不包含编码蛋白酶的基因的突变的宿主细胞相比具有提高的稳 定性。另一个方面包括通过以下方式制备异源多肽的方法：a)提供前述任一 实施方式的木霉属真菌细胞；b)培养宿主细胞以表达异源多肽；和c)纯化 异源多肽。在可以与前述实施方式组合的某些实施方式中，丝状真菌细胞进 一步包含载体蛋白。在某些实施方式中，载体蛋白是CBH1。
特别地，本发明涉及以下各项：
1.一种丝状真菌细胞，包含至少三种具有降低的活性的内源蛋白酶和 编码异源多肽的重组多核苷酸，其中所述多肽以比其中所述蛋白酶不具有降 低的活性的对应亲本丝状真菌细胞中该多肽的产生水平高至少两倍的水平产 生。
2.第1项所述的丝状真菌细胞，条件是当所述细胞是曲霉属细胞时， 所述总蛋白酶活性降低到其中所述蛋白酶不具有降低的活性的对应亲本曲霉 属细胞的总蛋白酶活性的50％或更低。
3.第1或2项所述的丝状真菌细胞，其中所述总蛋白酶活性降低到其 中所述蛋白酶不具有降低的活性的对应亲本丝状真菌细胞的总蛋白酶活性的 49％或更低，优选31％或更低。
4.第1-3项任一项所述的丝状真菌细胞，其中至少三个编码内源蛋白 酶的基因各自包含降低或消除相应蛋白酶活性的突变。
5.第1-4项任一项所述的丝状真菌细胞，其中至少四个编码内源蛋白 酶的基因各自包含降低或消除相应蛋白酶活性的突变。
6.前述任一项所述的丝状真菌细胞，其中所述真菌细胞是木霉属真菌 细胞、毁丝霉属真菌细胞、曲霉属真菌细胞、链孢霉属真菌细胞、青霉属细 胞或金孢子菌属真菌细胞。
7.前述任一项所述的丝状真菌细胞，其中所述真菌细胞是里氏木霉(Trichoderma reesei)。
8.第6或7项所述的丝状真菌细胞，其中所述细胞对于pep4蛋白酶 是野生型的。
9.第6、7或8项所述的丝状真菌细胞，其中所述细胞的至少以下三 种蛋白酶具有降低的活性或没有活性：pep1、tsp1和slp1或者gap1、slp1和 pep1。
10.第6或7项所述的丝状真菌细胞，其中所述细胞的选自以下的至少 三种或四种蛋白酶具有降低的或没有可检测的蛋白酶活性：pep1、pep2、pep3、 pep4、pep5、pep8、pep11、pep12、tsp1、slp1、slp2、slp3、slp7、gap1和gap2。
11.第10项所述的丝状真菌细胞，其中编码所述具有降低的或没有可 检测的蛋白酶活性的三种或四种蛋白酶的基因各自包含降低或消除相应蛋白 酶活性的突变。
12.第6或7项所述的丝状真菌细胞，其中所述细胞的至少八种蛋白酶 具有降低的活性或没有蛋白酶活性，编码所述八种蛋白酶的基因各自包含降 低或消除相应蛋白酶活性的突变，且所述八种蛋白酶是pep1、tsp1、slp1、gap1、 gap2、pep4、pep3和pep5。
13.第6或7项所述的丝状真菌细胞，其中所述细胞包含三到六种具有 降低的或没有可检测的活性的蛋白酶，且所述三到六种蛋白酶各选自pep1、 pep2、pep3、pep4、pep5、tsp1、slp1、slp2、slp3、gap1和gap2。
14.第6-13项中任一项所述的丝状真菌细胞，其中所述细胞包含编码 另外的蛋白酶的基因，其包含降低相应蛋白酶活性的突变，且所述另外的蛋 白酶选自pep7、pep8、pep11、pep12、tpp1、gap2、slp3、slp5、slp6、slp7 和slp8。
15.前述任一项所述的丝状真菌细胞，其中所述异源多肽是哺乳动物多 肽。
16.第15项所述的丝状真菌细胞，其中所述哺乳动物多肽是糖基化的。
17.第15项所述的丝状真菌细胞，其中所述哺乳动物多肽选自抗体及 其抗原结合片段、生长因子、干扰素、细胞因子和白介素。
18.第15项所述的丝状真菌细胞，其中所述哺乳动物多肽选自胰岛素 样生长因子1(IGF1)、人生长激素(hGH)和干扰素α2b(IFNα2b)。
19.前述任一项所述的丝状真菌细胞，其中所述真菌细胞进一步包含具 有降低的活性的ALG3。
20.第19项所述的丝状真菌细胞，其中所述编码ALG3的基因包含降 低或消除相应活性的突变。
21.第1-20项中任一项所述的丝状真菌细胞，其中所述真菌细胞进一 步包含编码α-1,2-甘露糖苷酶的多核苷酸。
22.前述任一项所述的丝状真菌细胞，进一步包含
N-乙酰葡糖胺基转移酶I催化结构域，和任选地
N-乙酰葡糖胺基转移酶II催化结构域。
23.第22项所述的丝状真菌细胞，进一步包含编码N-乙酰葡糖胺基转 移酶I催化结构域的第一多核苷酸和，任选地，编码N-乙酰葡糖胺基转移酶 II催化结构域的第二多核苷酸。
24.前述任一项所述的丝状真菌细胞，进一步包含编码甘露糖苷酶II 和/或半乳糖基转移酶的多核苷酸。
25.提高多肽的稳定性的方法，包括
a)提供前述任一项的丝状真菌细胞，和
b)培养所述细胞以使得表达所述异源多肽，
其中所述异源多肽与其中所述蛋白酶不具有降低的活性的对应亲本丝状 真菌细胞中产生的异源多肽相比表现出提高的稳定性。
26.制备异源多肽的方法，包括
a)提供第1-24项的丝状真菌细胞，
b)培养所述细胞以使得表达所述异源多肽，和
c)纯化所述异源多肽。
27.第25或26项所述的方法，其中所述丝状真菌细胞进一步包含载体 蛋白。
28.第27项所述的方法，其中所述载体蛋白是CHB1。
29.第26-28项中任一项所述的方法，其中所述培养是在包含蛋白酶抑 制剂的培养基中。
30.第26-29项中任一项所述的方法，其中所述培养是在包含选自SBT1 和胰凝乳蛋白酶抑制剂的一种或两种蛋白酶抑制剂的培养基中。
附图简要说明
图1描绘了显示从天冬氨酸蛋白酶的亲和柱纯化洗脱的级分的PAGE凝 胶。
图2描绘了显示将IgG与天冬氨酸蛋白酶孵育的结果的PAGE凝胶。
图3描绘了显示单蛋白酶删除菌株M181和M195的产生的DNA印迹分析。图3A描绘了pep1 ORF的预期信号：来自亲本M127的>8kb，没有来自 转化体的信号。图3B描绘了pep1 5'侧翼的预期信号：来自亲本M127的>8kb， 来自转化体的4kb。图3C描绘了pep1 3'侧翼的预期信号：来自亲本M127 的>8kb，来自转化体的4.2kb。
图4描绘了显示pep1缺失菌株M182中利妥昔单抗抗体的产生的DNA 印迹分析。图4A描绘了pep1 ORF的预期信号：来自亲本M169的>8kb，没 有来自转化体的信号。图4B描绘了bar的预期信号：来自转化体的1.0+1.7 kb，来自pTTv41的3.1kb，没有来自M169的信号。图4C描绘了bar的预 期信号：来自转化体的1.8+2.8kb，来自pTTv41的3.1kb，没有来自M169的信号。
图5描绘了显示含pep1菌株和Δpep1菌株的天冬氨酸蛋白酶纯化的峰级 分的蛋白质凝胶。
图6A-B描绘了免疫印迹，其说明从利妥昔单抗产生菌株M169删除pep2 蛋白酶提高了转化体206A(菌株M455)中(A)轻链和(B)重链的产生。代表 18kD的轻链片段和38kD的重链片段的条带在菌株M455中与亲本菌株 M169相比更强。
图7图示地描绘了来自利妥昔单抗产生菌株M169和pep2蛋白酶删除转 化体98A、116A、198A、201A和206A(M455)的上清液的蛋白酶活性。转 化体116A、198A和206A与其亲本菌株M169相比显示出降低的针对酪蛋白 的蛋白酶活性。
图8描绘了显示PEP3和PEP7的蛋白酶活性对MAB01重链和原始IGF-1 的影响的免疫印迹。图8A描绘了pH 5.5下蛋白酶活性对MAB01的影响。 图8A描绘了pH 4.5下蛋白酶活性对MAB01的影响。图8C描绘了pH 4.5 下蛋白酶活性对原始IGF-1的影响。
图9描绘了显示从SIP肽亲和柱纯化的含蛋白酶级分的PAGE凝胶。
图10描绘了显示对于MAB01重链的SIP蛋白酶活性的免疫印迹。
图11图示地描绘了有或没有抑制剂的情况下针对酪蛋白的蛋白酶活性。
图12描绘了显示在删除slp1、slp2、slp3和gap1各蛋白酶后MAB01重 链和轻链产生的水平的免疫印迹。图12A显示MAB01重链的产生。图12B显示MAB01轻链的产生。
图13图示地描绘了在删除slp1、slp2、slp3和gap1各蛋白酶后MAB01 重链和轻链产生的倍数提高。各条代表图12中显示的几个克隆的平均。
图14描绘了显示gap2删除菌株M244的MAB01产生水平的免疫印迹。 图14A显示MAB01重链(HC)的产生。图14B显示MAB01轻链(LC)的产生。
图15描绘了显示与含GAP2蛋白酶的毕赤酵母上清液孵育后MAB01抗 体的水平的免疫印迹。
图16描绘了显示人IgG1的蛋白酶降解水平的免疫印迹。
图17描绘了来自氨基苄脒柱的亲和纯化(纯化的级分)和来自上清液样 品(上清液)的MAB02抗体酶谱的结果。
图18描绘了Δpep1Δtsp1双重蛋白酶删除菌株M219的产生。图18A描 绘了tsp1ORF的预期信号：来自亲本M196的6.4kb。图18B描绘了tsp1 5' 侧翼的预期信号：来自转化体的3.9kb，来自M196的>8kb，来自pTTv72 的3.9kb。图18C描绘了tsp1 3'侧翼的预期信号：来自转化体的2.8kb，来自 M196的>8kb，来自pTTv72的3.9kb。
图19描绘了显示Δpep1Δtsp2双重删除菌株M194的产生的DNA印迹分 析。图19A描绘了tsp1 ORF的预期信号：来自亲本M181的kb。图19B描 绘了bar的预期信号：来自转化体的1.4+2.5kb，来自pTTv42的2.9kb，没 有来自M181的信号。图19C描绘了bar的预期信号：来自转化体的1.9+3.2 kb，来自pTTv42的2.9kb，没有来自M181的信号。
图20图示地描绘了来自各蛋白酶删除上清液和亲本菌株M124的培养上 清液的标准化蛋白酶活性数据。蛋白酶活性在前5个菌株中在pH 5.5下测量 和在后3个删除菌株中在pH 4.5下测量。蛋白酶活性是针对绿色荧光酪蛋白。 六重蛋白酶删除菌株仅具有野生型亲本菌株的6％和7重蛋白酶删除菌株的 蛋白酶活性比六重蛋白酶删除菌株活性低约40％。
图21A描绘了发酵上清液的氨基苄脒纯化级分的MAB02酶谱的结果。 图21B描绘了发酵上清液的氨基苄脒纯化级分的SDS PAGE凝胶(7％)。
图22描绘了包含蛋白酶的SBTI亲和纯化级分的MAB02酶谱分析的结 果。在蛋白酶降解MAB02抗体的情况中主要蛋白质水解活性表现为白色。 浓缩的级分3(cf3)和未浓缩的级分1-4(fl-f4)在酶谱凝胶中分析。
图23描绘了显示包含蛋白酶的SBTI亲和纯化级分的SDS PAGE凝胶。 浓缩的级分cf3和cf4显示于凝胶中。
图24描绘了显示SBTI纯化蛋白酶的利妥昔单抗重链降解水平的免疫印 迹。
图25描绘了显示与含枯草溶菌素的毕赤酵母上清液孵育过夜时的抗体 降解水平的免疫印迹。图25A显示利妥昔单抗重链的蛋白酶降解。图25B显示MAB01重链的蛋白酶降解。
图26描绘了显示三重蛋白酶删除菌株M277的产生的DNA印迹分析。 图26A描绘了slp1 ORF的预期信号：仅来自亲本(M219,M228)的6.5kb。图26B描绘了slp1 5'侧翼的预期信号：来自亲本的6.5kb，来自转化体的3.3kb， 来自质粒对照pTTv126的4.4kb。图26C描绘了slp1 3'侧翼的预期信号：来 自亲本的6.5kb，来自转化体的2.3kb，来自质粒对照pTTv126的4.4kb。
图27描绘了显示蛋白酶删除菌株上清液的活性的MAB02酶谱分析。染色的凝胶上的白色区域表示蛋白酶活性的区域。
图28图示地描绘了蛋白酶删除培养物上清液与野生型M124活性相比的 总蛋白酶活性。
图29描绘了显示四蛋白酶删除菌株M307的产生的DNA印迹分析。图29A描绘了gap1ORF的预期的信号：仅来自亲本(Mill 2A＝M306)的4kb。 图29B描绘了gap1 5'侧翼的预期的信号：来自亲本的5.5kb，来自转化体的 3.4kb，来自质粒对照pTTv117的4.1kb。图29C描绘了gap1 3'侧翼的预期 的信号：来自亲本的5.5kb，来自转化体的3.1kb，来自质粒对照pTTv117 的4.1kb。
图30图示地描绘了三重和四重删除菌株与野生型M124菌株相比的总蛋 白酶活性。
图31图示地描绘了M304三重删除菌株和M371四重删除菌株之间随时 间变化的蛋白酶活性。
图32描绘了显示四重蛋白酶删除菌株M369的产生的DNA印迹分析。 图32A描绘了gap2 ORF的预期信号：来自亲本(M307)的4.9kb。图32B描绘了gap2 5'侧翼的预期信号：来自亲本的4.9kb，来自转化体的2.3kb，来 自质粒对照pTTv145的2.3kb。图32C描绘了gap23'侧翼的预期信号：来自 亲本的4.9kb，来自转化体的3.8kb，来自质粒对照pTTv145的3.8kb。图32D描绘了显示从四重蛋白酶删除菌株M369产生pyr4-的DNA印迹分析， 从而产生菌株M381(克隆14)。预期的信号是gap2 5'侧翼：来自所有菌株的 1.5kb，来自质粒对照pTTv145的4.1kb。图32E描绘了显示从四重蛋白酶删 除菌株M369产生pyr4-的DNA印迹分析，从而产生菌株M381(克隆14)。 预期的信号是gap2 3'侧翼：来自M307的3.6kb，来自M369+环出(loopout) 克隆的2.7kb，来自质粒对照pTTv145的3.8kb。
图33图示地描绘了从利用4重蛋白酶删除菌株M307、5重蛋白酶删除 菌株M369及6重蛋白酶删除转化体10B、44B、97A、97B和120A进行的 摇瓶培养获取的第5天上清液的蛋白酶活性。荧光酪蛋白在pH 4.5的柠檬酸 盐缓冲液中与稀释的上清液孵育以检测蛋白酶活性。
图34描绘了显示6重蛋白酶删除菌株M396和M400的产生的DNA印 迹分析。图34A描绘了pep4 ORF的预期信号：来自M307和M369的6.3kb。 图34B描绘了pep4 ORF的预期信号：来自M307和M369的6.3kb，没有来 自转化体的信号。图34C描绘了pep4 5'侧翼的预期信号：来自M307和M369 的6.3kb，来自转化体的4.8kb，来自pTTv181的4.0kb。图34D描绘了pep43'侧翼的预期信号：来自M307和M369的6.3kb，来自转化体的2.1kb，来 自pTTv181的4.0kb。图34E描绘了显示从6重蛋白酶删除菌株M396产生 pyr4-的DNA印迹分析。预期的信号是pep4 3'侧翼：来自M307和M369的 6.3kb，来自再纯化转化体的2.1kb，来自环出克隆的4.9kb。
图35描绘了显示上清液蛋白酶体外产生的利妥昔单抗重链片段的量的免疫印迹。
图36描绘了显示来自SBTI处理的培养物和未处理对照的上清液样品的重链和轻链降解的免疫印迹。图36A显示重链的降解。图36B显示轻链的降解。
图37描绘了显示来自胰凝乳蛋白酶抑制剂和胃蛋白酶抑制剂A处理的培养物或来自未处理的对照培养物的上清液样品的重链和轻链降解水平的免 疫印迹。图37A显示轻链的降解。图37B显示重链的降解。
图38描绘了从里氏木霉培养物上清液纯化抗体的过程。
图39描绘了显示来自包含pep1蛋白酶删除的里氏木霉的抗体重链(HC) 和轻链(LC)的稳定性提高的免疫印迹。三种模型抗体在大的摇瓶上清液 (Δpep1和M124)和发酵上清液(pH 5.5；28℃；20g/L废谷物提取物(spent grain extract),60g/L乳糖)中测试。
图40描绘了显示来自包含tsp1蛋白酶删除的里氏木霉细胞的利妥昔单抗(Rx)重链的产生增加的免疫印迹。转化体12-2A和12-16A与亲本菌株相比清楚地显示更多的重链。
图41描绘了显示与来自三重蛋白酶删除菌株M277的上清液孵育过夜后 MAB01重链降解降低的免疫印迹。在第5天培养物上清液中孵育过夜后，与来自没有蛋白酶删除的对照菌株M124的上清液相比，在三重蛋白酶删除上 清液中发现2.5倍高的重链。当在7天培养上清液中孵育时，与来自对照菌 株M124的上清液相比，在三重蛋白酶删除上清液中发现4倍高的重链。
图42描绘了模型蛋白质的降解研究。来自6重蛋白酶删除菌株的未稀释 上清液在pH 4.2下用于掺入纯模型蛋白质中(0.05μg/μl)。掺有模型蛋白质 (0.05μg/μl)的50mM柠檬酸钠pH 4.0显示为缓冲液对照。掺杂的上清液和对 照在37℃下孵育20小时。10μl的各样品加载到18％SDS PAGE凝胶中。hGH 在22kD处，IFNα2b在19.4kD处和IGF1在7.5kD处。
图43描绘了来自6重蛋白酶删除菌株的上清液中MAB01抗体重链的稳 定性测试。MAB01抗体以0.05μg/μl存在于未稀释的上清液中。10μl的各样 品加载到4-20％SDS PAGE凝胶中。重链在来自6重蛋白酶删除菌株的上清 液中pH 4.2下37℃下20小时孵育后是稳定的。重链用TBST中1:30,000 稀释的抗重链IgG AP偶联的抗体(Sigma#A3188)检测。全长重链在凝胶上50 kD处。
图44描绘了来自具有或不具有抑制剂和补充剂的24孔培养物的人生长 激素的第4天样品。12μl的各上清液被加载。来自Acris的初级抗体，目录 #AM00401PU-N小鼠抗-hGH抗体(在TBST中稀释到2μg/ml)和1:10,000稀 释的BioRad(#170-6520)山羊抗小鼠IgG AP偶联二级抗体。hGH标准(200 ng)，Abcam目录#ab51232。全长hGH蛋白在22kD处。
图45描绘了里氏木霉、嗜热毁丝霉、粗糙链孢霉、产黄青霉、米曲霉、 构巢曲霉和黑曲霉的天冬氨酸蛋白酶的系统发生。比对利用Clustal Omega (http://www.ebi.ac.uk/Tools/msa/clustalo/)进行且树使用BLOSUM62采用平均 距离计算。
图46描绘了里氏木霉、嗜热毁丝霉、粗糙链孢霉、产黄青霉、米曲霉、 构巢曲霉和黑曲霉的枯草溶菌素蛋白酶的系统发生。比对利用Clustal Omega (http://www.ebi.ac.uk/Tools/msa/clustalo/)进行且树使用BLOSUM62利用平均 距离计算。“pyr”是指热解素(pyrolysin)，“prKsf3”是指蛋白酶K，亚族3； prtA、prtK、prU、prtF和prtBCI是指如Bryant等(2009)BMC Evolutionary Biology 9:168,doi:10.1186/1471-2148-9-168，图5和附加的文件no.8中所述 的亚族。
图47描绘了里氏木霉、嗜热毁丝霉、粗糙链孢霉、产黄青霉、米曲霉、 构巢曲霉和黑曲霉的谷氨酸蛋白酶的系统发生。比对利用Clustal Omega (http://www.ebi.ac.uk/Tools/msa/clustalo/)进行且树使用BLOSUM62利用平均 距离计算。
图48描绘了里氏木霉、嗜热毁丝霉、粗糙链孢霉、产黄青霉、米曲霉、 构巢曲霉和黑曲霉的sedolisin蛋白酶的系统发生。比对利用Clustal Omega (http://www.ebi.ac.uk/Tools/msa/clustalo/)进行且树使用BLOSUM62利用平均 距离计算。由于slp7类似于sedolisin蛋白酶，它包括在该树中。包括烟曲霉 序列以帮助确定sedolisins之间的关系。各蛋白酶前面的缩写sedA/B/C/D/E 是基于Reichard等(2006)APPLIED ANDENVIRONMENTAL MICROBIOLOGY,Vol.72,p.1739-1748，图4，由其中获取了与烟曲霉sedolisin (对应蛋白酶)的BLAST检索。
图49：A：表达质粒pTTv67和pTTv99的示意图。MAB01重链包含在 pTTv67载体中且轻链包含在pTTv99载体中。B：表达载体pTTv223的示意 图。MAB01重链和轻链包含在pTTv223载体中。
图50：A：MAB01产生菌株M507的分批补料发酵中pH 5.2下MAB01 轻链和重链产生的蛋白质印迹分析。使用的抗体是针对重链的Sigma A3188(左印迹)和针对轻链的SigmaA3813(右印迹)，都以1:10,000稀释。样 品代码表示按天计的发酵时间。0.1μl的上清液在两个印迹中加载到各道中。 B：pH 5.5下MAB01产生菌株M507的分批补料发酵中MAB01轻链和重链 产生的蛋白质印迹分析。使用的抗体是针对重链的Sigma A3188(左侧的印 迹)和针对轻链的Sigma A3813(右侧的印迹)，都以1:10,000稀释。样品代 码表示按天计的发酵时间。0.1μl的上清液在两个印迹中加载到各道中。
图51.pH 5.5下分批补料发酵bio00503b中菌株M304的MAB01轻链和 重链产生的蛋白质印迹分析。使用的抗体是针对重链的Sigma A3188和针对 轻链的Sigma A3813。来自M304发酵bio00477b的第8天包括作为对照。样 品代码表示按天计的发酵时间。0.1μl的上清液在两个印迹中加载。最上面的 印迹是重链和较下面的印迹是轻链。
图52.pTTv204 RNAi表达载体。
图53：表达敲减slp2表达的RNAi的菌株中检测MAB01重链产生的免 疫印迹。
图54A描绘了M401发酵的第3天样品的IFN-α2b表达水平的定量。1μl/2 μl/4μl上清液加载到4-20％SDS PAGE凝胶。免疫印迹用在TBST中稀释到 1μg/ml的Abcam(#ab9386)抗-IFN-α2b抗体进行。来自Bio-rad(#170-6520) 的二级抗体是在TBST中1:5000稀释的山羊抗-小鼠IgG AP偶联二级抗体。 蛋白质标准加载到凝胶上，对应于50ng、100ng和200ng的全长IFN-α 2b。 光密度定量使用Totallab Quant TL100软件进行。对于定量，2μl样品是最具 代表性的。全长IFN-α 2b对照(100ng)在19.3kD处和载体结合的IFN-α 2b 在70kDa处。
图54B描绘了M577和M652发酵培养的第3-6天样品的免疫印迹分析。 0.2μl的生长上清液加载到4-20％SDS PAGE凝胶。免疫印迹使用在TBST中 稀释到1μg/ml的Abcam(#ab9386)抗-IFN-α 2b抗体进行。来自Bio-rad (#170-6520)的二级抗体是在TBST中1:5000稀释的山羊抗-小鼠IgG AP偶联 二级抗体。全长IFN-α 2b对照(100ng)在19.3kD处和载体结合的IFN-α 2b 在70kDa处。
图55描绘了来自第4天(M577发酵)和第3天(M652发酵)样品的IFN-α 2b 表达水平的定量。各样品的0.05μl和0.1μl的上清液加载到4-20％SDS PAGE 凝胶。免疫印迹使用在TBST中稀释到1μg/ml的Abcam(#ab9386)抗-IFN-α 2b 抗体进行。来自Bio-rad(#170-6520)的二级抗体是在TBST中1:5000稀释的 山羊抗-小鼠IgG AP偶联二级抗体。蛋白质标准加载到凝胶上，对应于50ng、 100ng和200ng的全长IFN-α 2b。光密度定量使用Totallab QuantTL100软件 进行。对于定量，0.1μl样品是最具代表性的。全长IFN-α 2b对照(100ng)在19.3kD处和载体结合的IFN-α 2b在70kDa处。
详细说明
本发明涉及在至少三种蛋白酶具有降低的活性或没有活性的丝状真菌细 胞中产生重组异源多肽的改进的方法。本发明部分地基于以下令人惊异的发 现，即降低丝状真菌细胞中特定内源蛋白酶组合的活性提高多种重组表达的 异源蛋白质(如免疫球蛋白和生长因子)的表达和稳定性。尽管其它人产生 了具有一种或多种灭活的蛋白酶的木霉属真菌细胞，但他们未提供关于哪些 蛋白酶与提高特定类型的蛋白质(如哺乳动物蛋白质)的表达和稳定性最相 关的指导。例如，WO2011/075677公开了可以在木霉属中敲除的特定蛋白酶 且甚至公开了多种蛋白酶缺陷的木霉属真菌细胞。但是，WO2011/075677未 提供关于哪些蛋白酶对哺乳动物蛋白质(如免疫球蛋白或生长因子)的表达 和稳定性具有不利影响的任何指导，因为其中没有描述任何哺乳动物蛋白质 表达的实例。此外，WO2011/075677仅公开了单一真菌蛋白质在单一蛋白酶 缺陷的三种不同真菌菌株的各菌株中的异源表达。因此，本领域技术人员很 可能将WO2011/075677作为各单一蛋白质的灭活足以用于异源蛋白产生的 教导。Yoon等(2009,Appl.Microbiol Biotechnol 82:691-701,2010:Appl.Microbiol Biotechnol DOI 10.1007/s00253-010-2937-0)报告了用于在米曲霉中 产生异源蛋白的五重和十重蛋白酶基因破坏体(disruptant)的构建。10重蛋白 酶破坏体细胞提高凝乳酶产率仅3.8倍，尽管存在较高数目的破坏蛋白酶基 因。Van den Hombergh等报告了黑曲霉的三重蛋白酶基因破坏体。尽管数据 显示了蛋白酶活性的降低，但其中没有描述任何哺乳动物蛋白质产生的实例。
申请人出人意料地证明多种蛋白酶与丝状真菌细胞如木霉属真菌细胞 中总蛋白酶活性的降低、异源蛋白产生的增加和异源蛋白表达后的稳定相 关。特别地，发明人通过纯化蛋白酶和确定哪些蛋白酶具有与降解异源蛋白 质(如哺乳动物蛋白质)最相关的活性鉴定了在木霉属真菌细胞中实际表达 的蛋白酶(与仅在基因组中被编码相反)。另外地，发明人确认删除负责特 定蛋白酶活性的基因获得总蛋白酶活性的实质降低(这与包含这类删除的丝 状真菌细胞中就产生的蛋白质的量和质量方面来说蛋白质稳定性的提高相关)，并导致细胞中全长异源蛋白质产生的增加。还发现经工程化以降低至 少三种蛋白酶基因的活性的木霉属真菌细胞导致全长哺乳动物蛋白如抗体、 治疗性蛋白质或抗体变体(如Fab或单域抗体)的产生的预料不到的和协同 的增加。换句话说，产生的全长哺乳动物蛋白的量大于仅包含一种或两种蛋 白酶基因删除的木霉属真菌细胞产生的总量。因此，与WO2011/075677相 反，本发明人证明丝状真菌细胞如木霉属真菌细胞中完整异源蛋白质的产生 可以通过降低或消除细胞中至少三种蛋白酶的活性而实现。
因此，本公开的特定方面提供了通过具有降低的或没有至少三种蛋白酶 的活性产生提高水平的异源蛋白质的丝状真菌细胞，其中该细胞进一步包含 编码异源多肽的重组多核苷酸，该异源多肽的产生水平与其中蛋白酶不具有 降低的活性的对应亲本丝状真菌细胞中多肽的产生水平相比高至少两倍。换 句话说，所希望的异源蛋白产生水平的提高可通过将具有至少三种蛋白酶的 降低的活性的丝状真菌细胞中异源蛋白的产生水平与不具有这种降低的活性 但其它方面与表现出提高的水平的细胞相同的丝状真菌细胞中异源蛋白的产 生水平相比较确定。
本公开的其它方面提供了通过以下方式提高异源多肽稳定性的方法：a) 提供具有降低的或没有至少三种蛋白酶的活性的本公开的丝状真菌细胞，其 中该细胞进一步包含编码异源多肽的重组多核苷酸；和b)培养该细胞以表达 异源多肽，其中异源多肽与不包含编码蛋白酶的基因的突变的宿主细胞相比 具有提高的稳定性。
本公开的再其它方面提供了通过以下方式制备异源多肽的方法：a)提供 具有降低的或没有至少三种蛋白酶的活性的本公开的丝状真菌细胞，其中该 细胞进一步包含编码异源多肽的重组多核苷酸；b)培养宿主细胞以表达异源 多肽；和c)纯化异源多肽。
本公开的特定方面还提供通过具有降低的或没有至少三种蛋白酶的活性 而产生提高水平的哺乳动物多肽的木霉属真菌细胞，该至少三种蛋白酶选自 pep1、pep2、pep3、pep4、pep5、pep8、pep11、pep12、tsp1、slp1、slp2、gap1 和gap2，其中该细胞进一步包含编码哺乳动物多肽的重组多核苷酸，该哺乳 动物多肽的产生水平与其中蛋白酶不具有降低的活性的对应亲本木霉属真菌 细胞中多肽的产生水平相比高至少两倍。换句话说，所希望的异源蛋白产生 水平的提高可通过将具有至少三种蛋白酶的降低的活性的木霉属真菌细胞中 异源蛋白质的产生水平与不具有这种降低的活性但其它方面与表现出提高的 水平的细胞相同的木霉属真菌细胞中异源蛋白质的产生水平相比较确定。
本公开的其它方面提供了通过以下方式提高哺乳动物多肽稳定性的方 法：a)提供具有至少三种蛋白酶的降低的活性的本公开的木霉属真菌细胞， 其中该细胞进一步包含编码哺乳动物多肽的重组多核苷酸；和b)培养该细胞 以表达哺乳动物多肽，其中哺乳动物多肽与不包含编码蛋白酶的基因的突变 的宿主细胞相比具有提高的稳定性。
本公开的进一步的方面提供了通过以下方式制备哺乳动物多肽的方法： a)提供具有至少三种蛋白酶的降低的活性的本公开的木霉属真菌细胞，其中 该细胞进一步包含编码哺乳动物多肽的重组多核苷酸；b)培养宿主细胞以表 达哺乳动物多肽；和c)纯化哺乳动物多肽。
定义
如本文中所使用的，“免疫球蛋白”是指包含共价偶联在一起的重链和轻 链并能够特异性地与抗原结合的多聚蛋白质。免疫球蛋白分子是包括几种类 型的分子如IgM、IgD、IgG、IgA和IgE的大的分子家族。
如本文中所使用的，“抗体”是指完整的免疫球蛋白分子以及能够结合抗 原的其片段。这些包括杂合(嵌合)抗体分子(参见，例如Winter等Nature 349:293-99225,1991和美国专利No.4,816,567 226)、F(ab')2和F(ab)片段和 Fv分子、非共价异二聚体[227,228]、单链Fv分子(scFv)(参见，例如Huston 等Proc.Natl.Acad.Sci.U.S.A.85:5897-83,1988)、二聚和三聚的抗体片段构建 体、微抗体(参见，例如Pack等Biochem 31,1579-84,1992和Cumber等J. Immunology 149B,120-26,1992)、人源化抗体分子(参见，例如Riechmann等 Nature 332,323-27,1988；Verhoeyan等Science 239,1534-36,1988和GB 2,276,169)和从这类分子获得的任何功能性片段以及通过非常规过程如噬菌 体展示获得的抗体。优选地，抗体是单克隆抗体。获得单克隆抗体的方法是 本领域中公知的。
如本文中所使用的，“肽”和“多肽”是包括多个连续的聚合氨基酸残基的 氨基酸序列。为了本发明的目的，通常肽是包括最多50个氨基酸残基的那些 分子，和多肽包括超过50个氨基酸残基。肽或多肽可以包括修饰的氨基酸残 基、非由密码子编码的天然存在的氨基酸残基和非天然存在的氨基酸残基。 如本文中所使用的，“蛋白质”可以指任何尺寸的肽或多肽。
本发明的蛋白酶
本文描述的本发明涉及通过使得细胞中存在的至少三种蛋白酶具有降低 的或没有可检测的活性而产生提高水平的异源多肽如哺乳动物多肽的丝状真 菌细胞如木霉属真菌细胞。表达异源多肽的丝状真菌细胞中存在的这些蛋白 酶通常催化表达的重组多肽的显著降解。因此，通过降低或消除表达异源多 肽的丝状真菌细胞中的蛋白酶活性，表达的多肽的稳定性提高，导致多肽产 生水平的提高，且在一些情况中，导致产生的多肽的质量提高(例如，全长 多肽而不是降解的多肽)。
蛋白酶包括，但不限于天冬氨酸蛋白酶、胰蛋白酶样丝氨酸蛋白酶、枯 草溶菌素蛋白酶、谷氨酸蛋白酶和sedolisin蛋白酶。这类蛋白酶可以从丝状 真菌细胞中鉴定和分离并测试以确定是否其活性的降低影响丝状真菌细胞的 重组多肽的产生。用于鉴定和分离蛋白酶的方法是本领域中公知的，并包括， 但不限于亲和色谱、酶谱分析和凝胶电泳。鉴定的蛋白酶然后可以通过从表 达重组多肽如异源或哺乳动物多肽的丝状真菌细胞删除编码所鉴定的蛋白酶 的基因并确定是否该删除导致细胞总蛋白酶活性的降低(例如，降低到对应 亲本丝状真菌细胞总蛋白酶活性的49％或更低或者31％或更低的水平)和表 达的重组多肽的产生水平的提高(例如，比对应亲本丝状真菌细胞中的产生 水平高两倍)来进行测试。用于删除基因、测量总蛋白酶活性和测量产生的 蛋白质的水平的方法是本领域中公知的并包括本文中描述的方法。“对应亲本 丝状真菌细胞”指其中蛋白酶不具有降低的或消除的活性的对应细胞。
天冬氨酸蛋白酶
天冬氨酸蛋白酶是利用天冬氨酸残基水解多肽和蛋白质中的肽键的酶， 通常，天冬氨酸蛋白酶在其活性位点中包含两个高度保守的天冬氨酸残基(其 在酸性pH下具有最佳活性)。来自真核生物体如木霉属真菌的天冬氨酸蛋白 酶包括胃蛋白酶、组织蛋白酶和肾素。这类天冬氨酸蛋白酶具有双结构域的 结构，其被认为是由祖先基因重复产生的。与这种重复事件一致，各结构域 的总体折叠(overall fold)是相似的，尽管两个结构域的序列开始偏离。各 结构域贡献催化性天冬氨酸残基中的一个。活性位点位于天冬氨酸蛋白酶的 两个结构域形成的裂隙中。真核天冬氨酸蛋白酶进一步包括保守的二硫桥， 其可以帮助鉴别多肽为天冬氨酸蛋白酶。
在木霉属真菌细胞中已经鉴定了九种天冬氨酸蛋白酶：pep1(tre74156)、 pep2(tre53961)、pep3(tre121133)、pep4(tre77579)、pep5(tre81004)和pep7 (tre58669)、pep8(tre122076)、pep11(tre121306)和pep12(tre119876)。
Pep1
合适的pep1蛋白酶的实例包括，但不限于里氏木霉pep1(SEQ ID NO:1)、 有性哈茨木霉(Hypocrea lixii)gi|11558498(SEQ ID NO:2)、棘孢木霉 (Trichodermaasperellum)gi|47027997(SEQ ID NO:3)、深绿木霉(Trichoderma atroviride)jgi|Triat2l297887(SEQ ID NO:4)、绿木霉(Trichoderma virens) jgi|TriviGv29_8_2|81777(SEQ ID NO:5)、烟曲霉(Aspergillus fumigatus) jgi|Trire2|afm:Afu5g13300(SEQ IDNO:6)、米曲霉(Aspergillus oryzae) gi|94730408(SEQ ID NO:7)、金龟子绿僵菌(Metarhizium anisopliae) gi|322712783(SEQ ID NO:8)、玉米赤霉(Gibberella zeae)gi|46126795(SEQ ID NO:9)、Fusarium venenatum gi|18448713(SEQ ID NO:10)、尖孢镰刀菌 (Fusarium oxysporum)gi|342879173(SEQ ID NO:11)、Grosmannia clavigera gi|320591399(SEQ ID NO:12)、黑白轮枝菌(Verticillium alboatrum) gi|302422750(SEQID NO:13)、球毛壳霉(Chaetomium globosum)gi|116182964 (SEQ ID NO:14)、粗糙链孢霉(Neurospora crassa)gi|85110723(SEQ ID NO: 15)、四孢链孢菌(Neurosporatetrasperma)gi|336463990(SEQ ID NO:16)、嗜 热毁丝霉(Myceliophthorathermophila)gi367030924(SEQ ID NO:491)、产黄青 霉(Penicillium chrysogenum)gi255953325(SEQ ID NO:492)、黑曲霉 (Aspergillus niger)gi350639535(SEQ ID NO:493)、构巢曲霉(Aspergillus nidulans)gi67541436(SEQ ID NO:494)及其同源物。
因此，在某些实施方式中，本公开的蛋白酶(通常pep1蛋白酶)的氨基 酸序列与选自SEQ ID NO:1-16、SEQ ID NO:491-494的氨基酸序列具有50％ 或更高的同一性(例如，60％、65％、70％、75％、80％、85％、90％、91％ 、92％、93％、94％、95％、96％、97％、98％、99％、99.5％或更高)。在一 些实施方式中，蛋白酶与选自SEQ ID NO:1-16、SEQ ID NO:491-494的氨基 酸序列具有100％的同一性。
在一些实施方式中，pep1是里氏木霉pep1。里氏木霉pep1编码的氨基 酸序列示于SEQ ID NO:1中。在其它实施方式中，本公开的蛋白酶的氨基酸 序列与SEQ ID NO:1具有50％或更高的同一性(例如，60％、65％、70％、 75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、 99％、99.5％或更高)。在进一步的实施方式中，蛋白酶与SEQID NO:1具有 100％的同一性。
Pep2
合适的pep2蛋白酶的实例包括，但不限于里氏木霉pep2(SEQ ID NO: 182)、深绿木霉jgi|Triat2|142040(SEQ ID NO:183)、绿木霉 jgi|TriviGv29_8_2|53481(SEQ IDNO:184)、蛹虫草(Cordyceps militaris)CM01 gi|346326575(SEQ ID NO:185)、粗糙链孢霉gi 85111370(SEQ ID NO:495) 及其同源物。
因此，在某些实施方式中，本公开的蛋白酶(通常pep2蛋白酶)的氨基 酸序列与选自SEQ ID NO:182-185、SEQ ID NO:495的氨基酸序列具有50％ 或更高的同一性(例如，60％、65％、70％、75％、80％、85％、90％、91％ 、92％、93％、94％、95％、96％、97％、98％、99％、99.5％或更高)。在一 些实施方式中，蛋白酶与选自SEQ ID NO:182-185、SEQ ID NO:495的氨基 酸序列具有100％的同一性。
在一些实施方式中，pep2是里氏木霉pep2。里氏木霉pep2编码的氨基 酸序列示于SEQ ID NO:182中。在其它实施方式中，本公开的蛋白酶的氨基 酸序列与SEQ ID NO:182具有50％或更高的同一性(例如，60％、65％、70％、 75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、 99％、99.5％或更高)。在进一步的实施方式中，蛋白酶与SEQID NO:182具 有100％的同一性。
Pep3
合适的pep3蛋白酶的实例包括，但不限于里氏木霉pep3(SEQ ID NO: 17)、深绿木霉jgi|Triat2(SEQ ID NO:18)、绿木霉jgi|TriviGv29_8_2(SEQ ID NO:19)、有性哈茨木霉gi|145583125(SEQ ID NO:20)、棘孢木霉gi|51860175 (SEQ ID NO:21)、黑曲霉gi|317025164(SEQ ID NO:22)、烟曲霉(Aspergillus fumigatus)gi|159122534(SEQ ID NO:23)、黑曲霉gi|134054572(SEQ ID NO: 24)、蛹虫草gi|346318620(SEQ ID NO:25)、Glomerella graminicola gi|310800156(SEQ ID NO:26)、尖孢镰刀菌gi|342871221(SEQID NO:27)、 Grosmannia clavigera gi|320591121(SEQ ID NO:28)、灰葡萄孢霉(Botryotinia fuckeliana)gi|12002205(SEQ ID NO:29)、太瑞斯梭孢壳霉(Thielaviaterrestris) gi|346997107(SEQ ID NO:30)、核盘菌(Sclerotinia sclerotiorum)gi|156055954 (SEQ ID NO:31)、球毛壳霉gi|116197829(SEQ ID NO:32)、四孢链孢菌 gi|336472132(SEQ ID NO:33)、粗糙链孢霉gi|85102020(SEQ ID NO:34)、费 希新萨托菌(Neosartorya fischeri)gi|119467426(SEQ ID NO:35)、马尼弗青霉 菌(Penicilliummarneffei)gi|212534792(SEQ ID NO:36)、嗜热毁丝霉 gi367025909(SEQ ID NO:496)、产黄青霉gi255947264(SEQ ID NO:497)、米 曲霉391870123(SEQ ID NO:498)及其同源物。
因此，在某些实施方式中，本公开的蛋白酶(通常pep3蛋白酶)的氨基 酸序列与选自SEQ ID NO:17-36、SEQ ID NO:496-498的氨基酸序列具有 50％或更高的同一性(例如，60％、65％、70％、75％、80％、85％、90％、 91％、92％、93％、94％、95％、96％、97％、98％、99％、99.5％或更高)。在 一些实施方式中，蛋白酶与选自SEQ ID NO:17-36、SEQ ID NO:496-498的 氨基酸序列具有100％的同一性。
在一些实施方式中，pep3是里氏木霉pep3。里氏木霉pep3编码的氨基 酸序列示于SEQ ID NO:17中。在其它实施方式中，本公开的蛋白酶的氨基 酸序列与SEQ ID NO:17具有50％或更高的同一性(例如，60％、65％、70％、 75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、 99％、99.5％或更高)。在进一步的实施方式中，蛋白酶与SEQID NO:17具 有100％的同一性。
Pep4
合适的pep4蛋白酶的实例包括，但不限于里氏木霉pep4(SEQ ID NO: 37)、绿木霉jgi|TriviGv29_8_2(SEQ ID NO:38)、深绿木霉jgi|Triat2(SEQ ID NO:39)、黄绿木霉(Trichoderma aureoviride)gi|193735605(SEQ ID NO:40)、 黑曲霉gi|145232965(SEQID NO:41)、烟曲霉gi|70999520(SEQ ID NO:42)、 棒曲霉(Aspergillus clavatus)gi|121705756(SEQ ID NO:43)、Nectria haematococca gi|302899226(SEQ ID NO:44)、Glomerella graminicola gi|310796316(SEQ ID NO:45)、蛹虫草gi|346322842(SEQ IDNO:46)、玉米 赤霉gi|46138535(SEQ ID NO:47)、金龟子绿僵菌gi|322708430(SEQ ID NO:48)、尖孢镰刀菌gi|342882947(SEQ ID NO:49)、Metarhizium acridum gi|322700747(SEQID NO:50)、大丽轮枝菌(Verticillium dahliae)gi|346973691 (SEQ ID NO:51)、灰葡萄孢霉gi|154309857(SEQ ID NO:52)、球毛壳霉gi|1 16203505(SEQ ID NO:53)、太瑞斯梭孢壳霉gi|347001590(SEQ ID NO:54)、 稻瘟病菌(Magnaporthe oryzae)gi|39973863(SEQID NO:55)、黑孢块菌(Tuber melanosporum)gi|296417651(SEQ ID NO:56)、粗糙链孢霉gi|85094599(SEQ ID NO:57)、嗜热毁丝霉gi367031892gi255947264(SEQ ID NO:499)、产黄青 霉gi255936729gi255947264(SEQ ID NO:500)、米曲霉gi169770745 gi255947264(SEQID NO:501)、构巢曲霉gi67524891gi255947264(SEQ ID NO:502)及其同源物。
因此，在某些实施方式中，本公开的蛋白酶(通常pep4蛋白酶)的氨基 酸序列与选自SEQ ID NO:37-57、SEQ ID NO:499-502的氨基酸序列具有 50％或更高的同一性(例如，60％、65％、70％、75％、80％、85％、90％、 91％、92％、93％、94％、95％、96％、97％、98％、99％、99.5％或更高)。在 一些实施方式中，蛋白酶与选自SEQ ID NO:37-57、SEQ ID NO:499-502的 氨基酸序列具有100％的同一性。
在一些实施方式中，pep4是里氏木霉pep4。里氏木霉pep4编码的氨基 酸序列示于SEQ ID NO:37中。在其它实施方式中，本公开的蛋白酶的氨基 酸序列与SEQ ID NO:37具有50％或更高的同一性(例如，60％、65％、70％、 75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、 99％、99.5％或更高)。在进一步的实施方式中，蛋白酶与SEQID NO:37具 有100％的同一性。
Pep5
合适的pep5基因的实例包括，但不限于里氏木霉pep5(SEQ ID NO:58)、 绿木霉jgi|TriviGv29_8_2(SEQ ID NO:59)、深绿木霉jgi|Triat2|277859(SEQ ID NO:60)、Metarhizium acridum gi|322695806(SEQ ID NO:61)、尖孢镰刀菌 gi|156071418(SEQ IDNO:62)、蛹虫草gi|346324830(SEQ ID NO:63)、玉米 赤霉gi|46124247(SEQ ID NO:64)、大丽轮枝菌gi|346978752(SEQ ID NO: 65)、嗜热毁丝霉gi367019798(SEQ ID NO:503)及其同源物。
因此，在某些实施方式中，本公开的蛋白酶(通常pep5蛋白酶)的氨基 酸序列与选自SEQ ID NO:58-65、SEQ ID NO:503的氨基酸序列具有50％或 更高的同一性(例如，60％、65％、70％、75％、80％、85％、90％、91％、 92％、93％、94％、95％、96％、97％、98％、99％、99.5％或更高)。在一些 实施方式中，蛋白酶与选自SEQ ID NO:58-65、SEQ ID NO:503的氨基酸序 列具有100％的同一性。
在一些实施方式中，pep5是里氏木霉pep5。里氏木霉pep5编码的氨基 酸序列示于SEQ ID NO:58中。在其它实施方式中，本公开的蛋白酶的氨基 酸序列与SEQ ID NO:58具有50％或更高的同一性(例如，60％、65％、70％、 75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、 99％、99.5％或更高)。在进一步的实施方式中，蛋白酶与SEQID NO:58具 有100％的同一性。
Pep7
合适的pep7基因的实例包括，但不限于里氏木霉pep7(SEQ ID NO:186)、 深绿木霉jgi|Triat2(SEQ ID NO:187)、绿木霉jgi|TriviGv29_8_2(SEQ ID NO: 188)、Glomerella graminicola gi|310800487(SEQ ID NO:189)、Metarhizium acridum gi|322700577(SEQ ID NO:190)、太瑞斯梭孢壳霉gi|347003264(SEQ ID NO:191)、鹅掌柄孢壳(Podospora anserine)gi|171680938(SEQ ID NO: 192)、嗜热毛壳菌(Chaetomiumthermophilum)gi|340905460(SEQ ID NO:193)、 大丽轮枝菌gi|346975960(SEQ ID NO:194)、嗜热毁丝霉gi|347009870, gi367026634(SEQ ID NO:195)、粗糙链孢霉gi|85090078(SEQ ID NO:196)、 稻瘟病菌gi|39948622(SEQ ID NO:197)、球毛壳霉gi|116191517(SEQID NO: 198)、稻瘟病菌gi|39970765(SEQ ID NO:199)、构巢曲霉gi67522232(SEQ ID NO:504)、黑曲霉gi350630464(SEQ ID NO:505)、米曲霉gi317138074(SEQ ID NO:506)及其同源物。
因此，在某些实施方式中，本公开的蛋白酶(通常pep7蛋白酶)的氨基 酸序列与选自SEQ ID NO:186-199、SEQ ID NO:504-506的氨基酸序列具有 50％或更高的同一性(例如，60％、65％、70％、75％、80％、85％、90％、 91％、92％、93％、94％、95％、96％、97％、98％、99％、99.5％或更高)。在 一些实施方式中，蛋白酶与选自SEQ ID NO:186-199、SEQID NO:504-506 的氨基酸序列具有100％的同一性。
在一些实施方式中，pep7是里氏木霉pep7。里氏木霉pep7编码的氨基 酸序列示于SEQ ID NO:186中。在其它实施方式中，本公开的蛋白酶的氨基 酸序列与SEQ ID NO:186具有50％或更高的同一性(例如，60％、65％、70％、 75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、 99％、99.5％或更高)。在进一步的实施方式中，蛋白酶与SEQID NO:186具 有100％的同一性。
Pep8
合适的pep8基因的实例包括里氏木霉pep8EGR48424(SEQ ID NO: 507)、绿木霉EHK19238(SEQ ID NO:508)、深绿木霉EHK40047(SEQ ID NO: 509)、四孢链孢菌EG053367(SEQ ID NO:510)、嗜热毁丝霉XP_003658897 (SEQ ID NO:511)、粗糙链孢霉XP_965343(SEQ ID NO:512)、金龟子绿僵菌 EFZ03501(SEQ ID NO:513)、太瑞斯梭孢壳霉XP_003656869(SEQ ID NO: 514)、尖孢镰刀菌EGU79769(SEQ ID NO:515)和玉米赤霉XP_381566(SEQ ID NO:516)、稻瘟病菌XP_oo3714540.1(SEQ ID NO:517)、产黄青霉 XP_002557331(SEQ ID NO:518)、米曲霉XP_001822899.1(SEQ ID NO:519)、 构巢曲霉XP_664091.1(SEQ ID NO:520)、黑曲霉EHA24387.1(SEQ ID NO: 521)及其同源物。
因此，在某些实施方式中，本公开的蛋白酶(通常pep8蛋白酶)的氨基 酸序列与选自SEQ ID NO:507-521的氨基酸序列具有50％或更高的同一性 (例如，60％、65％、70％、75％、80％、85％、90％、91％、92％、93％、 94％、95％、96％、97％、98％、99％、99.5％或更高)。在一些实施方式中， 蛋白酶与选自SEQ ID NO:507-521的氨基酸序列具有具有100％的同一性。
在一些实施方式中，pep8是里氏木霉pep8。里氏木霉pep8编码的氨基 酸序列示于SEQ ID NO:507中。在其它实施方式中，本公开的蛋白酶的氨基 酸序列与SEQ ID NO:507具有50％或更高的同一性(例如，60％、65％、70％、 75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、 99％、99.5％或更高)。在进一步的实施方式中，蛋白酶与SEQID NO:507具 有100％的同一性。
Pep11
合适的pep11基因的实例包括，但不限于里氏木霉pep11 EGR49498(SEQ ID NO:522)、绿木霉EHK26120(SEQ ID NO:523)、深绿木霉EHK41756(SEQ ID NO:524)、Fusariumpseudograminearum EKJ74550(SEQ ID NO:525)、 Metarhizium acridum EFY91821(SEQID NO:526)和玉米赤霉XP_384151 (SEQ ID NO:527)、嗜热毁丝霉XP_003667387.1(SEQ IDNO:528)、粗糙链 孢霉XP_960328.1(SEQ ID NO:529)及其同源物。
因此，在某些实施方式中，本公开的蛋白酶(通常pep11蛋白酶)的氨 基酸序列与选自SEQ ID NO:522-529的氨基酸序列具有50％或更高的同一性 (例如，60％、65％、70％、75％、80％、85％、90％、91％、92％、93％、 94％、95％、96％、97％、98％、99％、99.5％或更高)。在一些实施方式中， 蛋白酶与选自SEQ ID NO:522-529的氨基酸序列具有100％的同一性。
在一些实施方式中，pep11是里氏木霉pep8。里氏木霉pep11编码的氨 基酸序列示于SEQ ID NO:522中。在其它实施方式中，本公开的蛋白酶的氨 基酸序列与SEQ ID NO:522具有50％或更高的同一性(例如，60％、65％、 70％、75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、 98％、99％、99.5％或更高)。在进一步的实施方式中，蛋白酶与SEQ ID NO: 522具有100％的同一性。
Pep12
合适的pep12基因的实例包括，但不限于里氏木霉pep12 EGR52517(SEQ ID NO:530)、绿木霉pep12 EHK18859(SEQ ID NO:531)、深绿木霉pep12 EHK45753(SEQ ID NO:532)、Fusarium pseudograminearum pep12 EKJ73392 (SEQ ID NO:533)、玉米赤霉pep12XP_388759(SEQ ID NO:534)和金龟子绿 僵菌pep12 EFY95489(SEQ ID NO:535)、粗糙链孢霉XP_964574.1(SEQ ID NO:536)、嗜热毁丝霉XP_003659978.1(SEQ ID NO:537)及其同源物。
因此，在某些实施方式中，本公开的蛋白酶(通常pep12蛋白酶)的氨 基酸序列与选自SEQ ID NO:530-537的氨基酸序列具有50％或更高的同一性 (例如，60％、65％、70％、75％、80％、85％、90％、91％、92％、93％、 94％、95％、96％、97％、98％、99％、99.5％或更高)。在一些实施方式中， 蛋白酶与选自SEQ ID NO:530-537的氨基酸序列具有100％的同一性。
在一些实施方式中，pep8是里氏木霉pep12。里氏木霉pep12编码的氨 基酸序列示于SEQ ID NO:530中。在其它实施方式中，本公开的蛋白酶的氨 基酸序列与SEQ ID NO:530具有50％或更高的同一性(例如，60％、65％、 70％、75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、 98％、99％、99.5％或更高)。在进一步的实施方式中，蛋白酶与SEQ ID NO: 530具有100％的同一性。
胰蛋白酶样丝氨酸蛋白酶
胰蛋白酶样丝氨酸蛋白酶是具有与胰蛋白酶类似的底物特异性的酶。胰 蛋白酶样丝氨酸蛋白酶利用丝氨酸残基水解多肽和蛋白质中的肽键。通常， 胰蛋白酶样丝氨酸蛋白酶切割带正电的氨基酸残基之后的肽键。来自真核生 物体如木霉属真菌的胰蛋白酶样丝氨酸蛋白酶包括胰蛋白酶1、胰蛋白酶2 和中胰蛋白酶(mesotrypsin)。这类胰蛋白酶样丝氨酸蛋白酶一般包含三个氨基 酸(如组氨酸、天冬氨酸和丝氨酸)的催化三联体(catalytic triad)，其形成使 得活性位点丝氨酸亲核的电荷中继(charge relay)。真核生物胰蛋白酶样丝氨酸 蛋白酶进一步包括由甘氨酸和丝氨酸的骨架酰胺氢原子形成的“氧阴离子 穴”，其可以帮助鉴别多肽为胰蛋白酶样丝氨酸蛋白酶。
在木霉属真菌细胞中已经鉴定了一种胰蛋白酶样丝氨酸蛋白酶：tsp1(tre73897)。如以下讨论的，已证明tsp1对重组多肽如免疫球蛋白的表达具有 显著影响。
如以下实施例3中讨论的，丝氨酸蛋白酶从木霉属纯化并显示为具有降 解哺乳动物蛋白的多种蛋白酶活性。在这些活性中，tsp1确认为胰蛋白酶样 丝氨酸蛋白酶。tsp1蛋白酶基因然后从木霉属真菌细胞删除并证明删除tsp1 实现了总蛋白酶活性的显著降低，从而导致该细胞产生的哺乳动物蛋白的稳 定性提高。
合适的tsp1蛋白酶的实例包括，但不限于里氏木霉tsp1(SEQ ID NO:66) 、深绿木霉jgi|Triat2|298187(SEQ ID NO:67)、jgi|TriviGv29_8_2(SEQ ID NO: 68)、有性哈茨木霉gi|145583579(SEQ ID NO:69)、有性哈茨木霉 gi|63025000(SEQ ID NO:70)、核盘菌gi|156052735(SEQ ID NO:71)、灰葡 萄孢霉gi|154314937(SEQ ID NO:72)、颖枯壳针孢(Phaeosphaeria nodorum) gi|169605891(SEQ ID NO:73)、Leptosphaeria maculans gi|312219044(SEQ ID NO:74)、大丽轮枝菌gi|37992773(SEQ ID NO:75)、炭色孢腔菌(Cochliobolus carbonum)gi|1072114(SEQ ID NO:76)、Metarhizium acridum gi|322695345 (SEQ ID NO:77)、金龟子绿僵菌gi|4768909(SEQ ID NO:78)、gi|464963(SEQID NO:79)、玉米赤霉gi|46139299(SEQ ID NO:80)、金龟子绿僵菌(SEQ ID NO:81)、构巢曲霉gi67523821(SEQ ID NO:538)及其同源物。
因此，在某些实施方式中，本公开的蛋白酶(通常tsp1蛋白酶)的氨基 酸序列与选自SEQ ID NO:66-81、SEQ ID NO:538的氨基酸序列具有50％或 更高的同一性(例如，60％、65％、70％、75％、80％、85％、90％、91％、 92％、93％、94％、95％、96％、97％、98％、99％、99.5％或更高)。在一些 实施方式中，蛋白酶与选自SEQ ID NO:66-81、SEQ ID NO:538的氨基酸序 列具有100％的同一性。
在一些实施方式中，tsp1是里氏木霉tsp1。里氏木霉tsp1编码的氨基酸 序列示于SEQ ID NO:66中。在其它实施方式中，本公开的蛋白酶的氨基酸 序列与SEQ ID NO:66具有50％或更高的同一性(例如，60％、65％、70％、 75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、 99％、99.5％或更高)。在进一步的实施方式中，蛋白酶与SEQID NO:66具 有100％的同一性。
枯草溶菌素蛋白酶
枯草溶菌素蛋白酶是具有与枯草溶菌素类似的底物特异性的酶。枯草溶 菌素蛋白酶利用丝氨酸残基水解多肽和蛋白质中的肽键。一般地，枯草溶菌 素蛋白酶是包含三个氨基酸天冬氨酸、组氨酸和丝氨酸的催化三联体的丝氨 酸蛋白酶。这些催化性残基的排列是来自地衣芽孢杆菌(Bacillus licheniformis) 的原型枯草溶菌素共有的。来自真核生物体如木霉属真菌的枯草溶菌素蛋白 酶包括弗林蛋白酶、MBTPS1和TPP2。真核生物胰蛋白酶样丝氨酸蛋白酶进 一步包括氧阴离子穴中的天冬氨酸残基。枯草溶菌素蛋白酶slp7也类似于 sedolisin蛋白酶tpp1。
在木霉属真菌细胞中已经鉴定了七种枯草溶菌素蛋白酶：slp1 (tre51365)、slp2(tre123244)、slp3(tre123234)、slp5(tre64719)、slp6(tre121495)、 slp7(tre123865)和slp8(tre58698)。
Slp1
合适的slp1蛋白酶的实例包括，但不限于里氏木霉slp1(SEQ ID NO:82) 、深绿木霉jgi|Triat2(SEQ ID NO:83)、深绿木霉jgi|Triat2(SEQ ID NO:84) 、绿木霉jgi|TriviGv29_8_2(SEQ ID NO:85)、有性哈茨木霉gi|145583581 (SEQ ID NO:86)、Metarhizium acridum gi|322694632(SEQ ID NO:87)、尖孢 镰刀菌gi|342877080(SEQ IDNO:88)、玉米赤霉gi|46139915(SEQ ID NO: 89)、Epichloe festucae gi|170674476(SEQID NO:90)、Nectria haematococca gi|302893164(SEQ ID NO:91)、Sordaria macrosporegi|336266150(SEQ ID NO:92)、Glomerella graminicola gi|310797947(SEQ ID NO:93)、四孢链孢菌 gi|336469805(SEQ ID NO:94)、粗糙链孢霉gi|85086707(SEQ ID NO:95)、 稻瘟病菌gi|145608997(SEQ ID NO:96)、球毛壳霉gi|116208730(SEQ ID NO:97)、嗜热毁丝霉gi367029081(SEQ ID NO:539)及其同源物。
因此，在某些实施方式中，本公开的蛋白酶(通常slp1蛋白酶)的氨基 酸序列与选自SEQ ID NO:82-97、SEQ ID NO:539的氨基酸序列具有50％或 更高的同一性(例如，60％、65％、70％、75％、80％、85％、90％、91％、 92％、93％、94％、95％、96％、97％、98％、99％、99.5％或更高)。在一些 实施方式中，蛋白酶与选自SEQ ID NO:82-97、SEQ ID NO:539的氨基酸序 列具有100％的同一性。
在一些实施方式中，slp1是里氏木霉slp1。里氏木霉slp1编码的氨基酸 序列示于SEQ ID NO:82中。在其它实施方式中，本公开的蛋白酶的氨基酸 序列与SEQ ID NO:82具有50％或更高的同一性(例如，60％、65％、70％、 75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、 99％、99.5％或更高)。在进一步的实施方式中，蛋白酶与SEQID NO:82具 有100％的同一性。
Slp2
合适的slp2蛋白酶的实例包括，但不限于里氏木霉slp2(SEQ ID NO:98)、 深绿木霉jgi|Triat2(SEQ ID NO:99)、绿木霉jgi|TriviGv29_8_2(SEQ ID NO: 100)、有性哈茨木霉gi|115111226(SEQ ID NO:101)、烟曲霉gi|70997972(SEQ ID NO:102)、Nectriahaematococca gi|302915240(SEQ ID NO:103)、玉米赤 霉gi|46105128(SEQ ID NO:104)、虫花棒束孢(Isaria farinose)gi|68165000 (SEQ ID NO:105)、Glomerella graminicolagi|310797854(SEQ ID NO:106)、 Epichloe festucae gi|170674491(SEQ ID NO:107)、Metarhizium acridum gi|322697754(SEQ ID NO:108)、支顶孢属(Acremonium sp.)F11177 gi|147225254(SEQ ID NO:109)、Ophiostoma piliferum gi|15808807(SEQ IDNO:110)、四孢链孢菌gi|336463649(SEQ ID NO:111)、嗜热毛壳菌 gi|340992600(SEQ IDNO:112)、Metarhizium flavoviride gi|254351265(SEQ ID NO:113)、鹅掌柄孢壳gi|171680111(SEQ ID NO:114)、稻瘟病菌 gi|39943180(SEQ ID NO:115)、核盘菌gi|156058540(SEQ ID NO:116)、柄篮 状菌(Talaromyces stipitatus)gi|242790441(SEQ IDNO:117)、嗜热毁丝霉 gi367021472(SEQ ID NO:540)、黑曲霉gi145237646(SEQ ID NO:541)、米 曲霉gi169780712(SEQ ID NO:542)、产黄青霉gi255955889(SEQ ID NO: 543)、构巢曲霉gi259489544(SEQ ID NO:544)、粗糙链孢霉gi85084841 (SEQ ID NO:545)及其同源物。
因此，在某些实施方式中，本公开的蛋白酶(通常slp2蛋白酶)的氨基 酸序列与选自SEQ ID NO:98-117、SEQ ID NO:540-545的氨基酸序列具有 50％或更高的同一性(例如，60％、65％、70％、75％、80％、85％、90％、 91％、92％、93％、94％、95％、96％、97％、98％、99％、99.5％或更高)。在 一些实施方式中，蛋白酶与选自SEQ ID NO:98-117、SEQ ID NO:540-545的 氨基酸序列具有100％的同一性。
在一些实施方式中，slp2是里氏木霉slp2。里氏木霉slp2编码的氨基酸 序列示于SEQ ID NO:98中。在其它实施方式中，本公开的蛋白酶的氨基酸 序列与SEQ ID NO:98具有50％或更高的同一性(例如，60％、65％、70％、 75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、 99％、99.5％或更高)。在进一步的实施方式中，蛋白酶与SEQID NO:98具 有100％的同一性。
Slp3
合适的slp3蛋白酶的实例包括，但不限于里氏木霉slp2(SEQ ID NO: 166)、深绿木霉jgi|Triat2(SEQ ID NO:167)、绿木霉jgi|TriviGv29_8_2(SEQ ID NO:168)、康宁肉座菌(Hypocrea koningii)gi|124295071(SEQ ID NO:169)、淡 紫紫孢菌(Purpureocilliumlilacinum)gi|130750164(SEQ ID NO:170)、金龟子 绿僵菌gi|16215677(SEQ ID NO:171)、洛斯里被毛孢(Hirsutella rhossiliensis) gi|90655148(SEQ ID NO:172)、Tolypocladium inflatum gi|18542429(SEQ ID NO:173)、Metacordycepschlamydosporia gi|19171215(SEQ ID NO:174)、蛹虫 草gi|346321368(SEQ ID NO:175)、镰孢菌属(Fusarium sp.)gi|628051(SEQ ID NO:176)、四孢链孢菌gi|336471881(SEQ IDNO:177)、球毛壳霉gi|116197403 (SEQ ID NO:178)、粗糙链孢霉gi|85084841(SEQ ID NO:179)、尖孢镰刀菌 gi|56201265(SEQ ID NO:180)、玉米赤霉gi|46114268(SEQ ID NO:181)、嗜 热毁丝霉gi367026259(SEQ ID NO:546)、构巢曲霉gi67538776(SEQ ID NO:547)、米曲霉gi169771349(SEQ ID NO:222)、黑曲霉gi470729(SEQ ID NO: 223)及其同源物。
因此，在某些实施方式中，本公开的蛋白酶(通常slp3蛋白酶)的氨基 酸序列与选自SEQ ID NO:166-181、SEQ ID NO:546-547、SEQ ID NO: 222-223的氨基酸序列具有50％或更高的同一性(例如，60％、65％、70％、 75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％ 、99％、99.5％或更高)。在一些实施方式中，蛋白酶与选自SEQ IDNO: 166-181、SEQ ID NO:546-547、SEQ ID NO:222-223的氨基酸序列具有100％ 的同一性。
在一些实施方式中，slp3是里氏木霉slp3。里氏木霉slp3编码的氨基酸 序列示于SEQ ID NO:166中。在其它实施方式中，本公开的蛋白酶的氨基酸 序列与SEQ ID NO:166具有50％或更高的同一性(例如，60％、65％、70％、 75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、 99％、99.5％或更高)。在进一步的实施方式中，蛋白酶与SEQID NO:166具 有100％的同一性。
Slp5
合适的slp5蛋白酶的实例包括，但不限于里氏木霉slp5(SEQ ID NO: 200)、深绿木霉jgi|Triat2(SEQ ID NO:201)、绿木霉jgi|TriviGv29_8_2(SEQ ID NO:202)、有性哈茨木霉gi|118161442(SEQ ID NO:203)、尖孢镰刀菌gi|342883549(SEQ ID NO:204)、玉米赤霉gi|46135733(SEQ ID NO:205)、 Glomerella graminicola gi|310796396(SEQ ID NO:206)、Nectria haematococca gi|302927954(SEQ ID NO:207)、蛹虫草gi|346319783(SEQID NO:208)、粗 糙链孢霉gi|85094084(SEQ ID NO:209)、四孢链孢菌gi|336467281(SEQID NO:210)、大丽轮枝菌gi|346971706(SEQ ID NO:211)、太瑞斯梭孢壳霉 gi|347001418(SEQ ID NO:212)、稻瘟病菌gi|145605493(SEQ ID NO:213)、 嗜热毁丝霉gi367032200(SEQ ID NO:548)、产黄青霉gi62816282(SEQ ID NO: 549)及其同源物。
因此，在某些实施方式中，本公开的蛋白酶(通常slp5蛋白酶)的氨基 酸序列与选自SEQ ID NO:200-213、SEQ ID NO:548-549的氨基酸序列具有 50％或更高的同一性(例如，60％、65％、70％、75％、80％、85％、90％、 91％、92％、93％、94％、95％、96％、97％、98％、99％、99.5％或更高)。在 一些实施方式中，蛋白酶与选自SEQ ID NO:200-213、SEQID NO:548-549 的氨基酸序列具有100％的同一性。
在一些实施方式中，slp5是里氏木霉slp5。里氏木霉slp5编码的氨基酸 序列示于SEQ ID NO:200中。在其它实施方式中，本公开的蛋白酶的氨基酸 序列与SEQ ID NO:200具有50％或更高的同一性(例如，60％、65％、70％、 75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、 99％、99.5％或更高)。在进一步的实施方式中，蛋白酶与SEQID NO:200具 有100％的同一性。
Slp6
合适的slp6蛋白酶的实例包括，但不限于里氏木霉slp6(SEQ ID NO: 214)、深绿木霉jgi|Triat2(SEQ ID NO:215)、绿木霉jgi|TriviGv29_8_2(SEQ ID NO:216)、绿色木霉菌(Hypocrea virens)gi|29421423(SEQ ID NO:217)、有性 哈茨木霉gi|145583127(SEQID NO:218)、钩状木霉(Trichoderma hamatum) gi|30144643(SEQ ID NO:219)、烟曲霉gi|2295(SEQ ID NO:220)、土曲霉 (Aspergillus terreus)gi|115391147(SEQ ID NO:221)、米曲霉gi|169771349 (SEQ ID NO:222)、黑曲霉gi|470729(SEQ ID NO:223)、Glomerellagraminicola gi|310794714(SEQ ID NO:224)、玉米赤霉gi|46114946(SEQ ID NO:225)、尖孢镰刀菌gi|342873942(SEQ ID NO:226)、Nectria haematococca gi|302884541 (SEQ IDNO:227)、费希新萨托菌(Neosartorya fischeri)gi|119500190(SEQ ID NO:228)、黑白轮枝菌gi|302413161(SEQ ID NO:229)、Glomerella graminicola gi|310790144(SEQ IDNO:230)、粗糙链孢霉gi85090020(SEQ ID NO:550)及 其同源物。
因此，在某些实施方式中，本公开的蛋白酶(通常slp6蛋白酶)的氨基 酸序列与选自SEQ ID NO:214-230、SEQ ID NO:550的氨基酸序列具有50％ 或更高的同一性(例如，60％、65％、70％、75％、80％、85％、90％、91％ 、92％、93％、94％、95％、96％、97％、98％、99％、99.5％或更高)。在一 些实施方式中，蛋白酶与选自SEQ ID NO:214-230、SEQ ID NO:550的氨基 酸序列具有100％的同一性。
在一些实施方式中，slp6是里氏木霉slp6。里氏木霉slp6编码的氨基酸 序列示于SEQ ID NO:214中。在其它实施方式中，本公开的蛋白酶的氨基酸 序列与SEQ ID NO:214具有50％或更高的同一性(例如，60％、65％、70％、 75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、 99％、99.5％或更高)。在进一步的实施方式中，蛋白酶与SEQID NO:214具 有100％的同一性。
Slp7
合适的slp7蛋白酶的实例包括，但不限于里氏木霉slp7(SEQ ID NO: 231)、深绿木霉jgi|Triat2(SEQ ID NO:232)、绿木霉jgi|TriviGv29_8_2(SEQ ID NO:233)、金龟子绿僵菌gi|322710320(SEQ ID NO:234)、Nectria haematococca gi|302915000(SEQ ID NO:235)、嗜热毁丝霉gi|347009020, gi367024935(SEQ ID NO:236)、玉米赤霉gi|46137655(SEQ ID NO:237)、太 瑞斯梭孢壳霉gi|346996549(SEQ ID NO:238)、稻瘟病菌gi|145610733(SEQ ID NO:239)、构巢曲霉gi67541991(SEQ ID NO:551)、产黄青霉gi255933786 (SEQ ID NO:552)、黑曲霉gi317036543(SEQ ID NO:553)、米曲霉gi169782882(SEQ ID NO:554)、粗糙链孢霉gi85109979(SEQ ID NO:555)及 其同源物。
因此，在某些实施方式中，本公开的蛋白酶(通常slp7蛋白酶)的氨基 酸序列与选自SEQ ID NO:231-239、SEQ ID NO:551-555的氨基酸序列具有 50％或更高的同一性(例如，60％、65％、70％、75％、80％、85％、90％、 91％、92％、93％、94％、95％、96％、97％、98％、99％、99.5％或更高)。在 一些实施方式中，蛋白酶与选自SEQ ID NO:231-239、SEQID NO:551-555 的氨基酸序列具有100％的同一性。
在一些实施方式中，slp7是里氏木霉slp7。里氏木霉slp7编码的氨基酸 序列示于SEQ ID NO:231中。在其它实施方式中，本公开的蛋白酶的氨基酸 序列与SEQ ID NO:231具有50％或更高的同一性(例如，60％、65％、70％、 75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、 99％、99.5％或更高)。在进一步的实施方式中，蛋白酶与SEQID NO:231具 有100％的同一性。
Slp8
合适的slp8蛋白酶的实例包括，但不限于里氏木霉slp8(SEQ ID NO: 240)、深绿木霉jgi|Triat2|198568(SEQ ID NO:241)、绿木霉 jgi|TriviGv29_8_2|33902(SEQ IDNO:242)及其同源物。
因此，在某些实施方式中，本公开的蛋白酶的氨基酸序列与选自SEQ ID NO:240-242的氨基酸序列具有50％或更高的同一性(例如，60％、65％、70％ 、75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、 98％、99％、99.5％或更高)。在一些实施方式中，蛋白酶与选自SEQ ID NO: 240-242的氨基酸序列具有100％的同一性。
在一些实施方式中，slp8是里氏木霉slp8。里氏木霉slp8编码的氨基酸 序列示于SEQ ID NO:240中。在其它实施方式中，本公开的蛋白酶的氨基酸 序列与SEQ ID NO:240具有50％或更高的同一性(例如，60％、65％、70％、 75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、 99％、99.5％或更高)。在进一步的实施方式中，蛋白酶与SEQID NO:240具 有100％的同一性。
谷氨酸蛋白酶
谷氨酸蛋白酶是水解多肽和蛋白质中的肽键的酶。谷氨酸蛋白酶对胃 蛋白酶抑制剂A是不敏感的，且因此有时称为胃蛋白酶抑制剂不敏感的酸性 蛋白酶。尽管谷氨酸蛋白酶先前与天冬氨酸蛋白酶一起分组且通常统称为酸 性蛋白酶，目前发现谷氨酸蛋白酶具有与天冬氨酸蛋白酶非常不同的活性位 点残基。
在木霉属真菌细胞中已经鉴定了两种谷氨酸蛋白酶：gap1(tre69555)和 gap2(tre106661)。
Gap1
合适的gap1蛋白酶的实例包括，但不限于里氏木霉gap1(SEQ ID NO: 118)、深绿木霉jgi|Triat2|40863(SEQ ID NO:119)、绿木霉 jgi|TriviGv29_8_2|192684(SEQ IDNO:120)、黄曲霉gi|238499183(SEQ ID NO:121)、黑曲霉gi|145251555(SEQ ID NO:122)、土曲霉gi|115491521 (SEQ ID NO:123)、gi|37154543(SEQ ID NO:124)、gi|48425531(SEQID NO: 125)、gi|351873(SEQ ID NO:126)、太瑞斯梭孢壳霉gi|346997245(SEQ ID NO:127)、产黄曲霉gi|255940586(SEQ ID NO:128)、嗜热毁丝霉 gi367026504(SEQ ID NO:574)、米曲霉gi317150886(SEQ ID NO:575)、粗 糙链孢霉gi85097968(SEQ ID NO:576)、黑曲霉gi131056(SEQ ID NO:577) 、产黄青霉gi255930123(SEQ ID NO:578)、黑曲霉gi145236956(SEQ ID NO:579)、米曲霉gi169772955(SEQ ID NO:580)、黑曲霉gi145249222(SEQ ID NO:581)、构巢曲霉gi67525839(SEQ ID NO:582)、米曲霉gi169785367 (SEQ IDNO:583)、产黄青霉gi255955319(SEQ ID NO:584)、嗜热毁丝霉 gi367019352(SEQ ID NO:585)、米曲霉gi391863974(SEQ ID NO:586)、嗜 热毁丝霉gi367024513(SEQ ID NO:587)及其同源物。
因此，在某些实施方式中，本公开的蛋白酶(通常gap1蛋白酶)的氨基 酸序列与选自SEQ ID NO:118-128、SEQ ID NO:574-587的氨基酸序列具有 50％或更高的同一性(例如，60％、65％、70％、75％、80％、85％、90％、 91％、92％、93％、94％、95％、96％、97％、98％、99％、99.5％或更高)。在 一些实施方式中，蛋白酶与选自SEQ ID NO:118-128、SEQID NO:574-587 的氨基酸序列具有100％的同一性。
在一些实施方式中，gap1是里氏木霉gap1。里氏木霉gap1编码的氨基 酸序列示于SEQ ID NO:118中。在其它实施方式中，本公开的蛋白酶的氨基 酸序列与SEQ ID NO:118具有50％或更高的同一性(例如，60％、65％、70％、 75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、 99％、99.5％或更高)。在进一步的实施方式中，蛋白酶与SEQID NO:118具 有100％的同一性。
Gap2
合适的gap2蛋白酶的实例包括，但不限于里氏木霉gap2(SEQ ID NO: 129)、深绿木霉jgi|Triat2|298116(SEQ ID NO:130)、绿木霉 jgi|TriviGv29_8_2|30331(SEQ IDNO:131)、jgi|TriviGv29_8_2|225131(SEQ ID NO:132)、黄曲霉gi|238499183(SEQ ID NO:133)、黑曲霉gi|145251555(SEQ ID NO:134)、构巢曲霉gi|67901056(SEQ ID NO:135)、棒曲霉gi|121711990 (SEQ ID NO:136)、烟曲霉gi|70986250(SEQ ID NO:137)、马尼弗青霉菌 gi|212534108(SEQ ID NO:138)、柄篮状菌gi|242789335(SEQ ID NO:139)、Grosmannia clavigera gi|320591529(SEQ ID NO:140)、费希新萨托菌 gi|119474281(SEQ ID NO:141)、马尼弗青霉菌gi|212527274(SEQ ID NO: 142)、产黄曲霉gi|255940586(SEQ ID NO:143)、gi|131056(SEQ ID NO:144)、 嗜热毁丝霉gi367030275(SEQ ID NO:588)及其同源物。
因此，在某些实施方式中，本公开的蛋白酶(通常gap2蛋白酶)的氨基 酸序列与选自SEQ ID NO:129-144、SEQ ID NO:588的氨基酸序列具有50％ 或更高的同一性(例如，60％、65％、70％、75％、80％、85％、90％、91％ 、92％、93％、94％、95％、96％、97％、98％、99％、99.5％或更高)。在一 些实施方式中，蛋白酶与选自SEQ ID NO:129-144、SEQ ID NO:588的氨基 酸序列具有100％的同一性。
在一些实施方式中，gap2是里氏木霉gap2。里氏木霉gap2编码的氨基 酸序列示于SEQ ID NO:129中。在其它实施方式中，本公开的蛋白酶的氨基 酸序列与SEQ ID NO:129具有50％或更高的同一性(例如，60％、65％、70％、 75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、 99％、99.5％或更高)。在进一步的实施方式中，蛋白酶与SEQID NO:129具 有100％的同一性。
Sedolisin蛋白酶
Sedolisin蛋白酶是利用丝氨酸残基水解多肽和蛋白质中的肽键的酶。Sedolisin蛋白酶一般包含独特的丝氨酸、谷氨酸和天冬氨酸催化三联体。 Sedolisin蛋白酶也包含氧阴离子穴中的天冬氨酸残基。来自真核生物体如木 霉属真菌的sedolisin蛋白酶包括三肽基肽酶。
合适的tpp1蛋白酶的实例包括，但不限于里氏木霉tpp1(SEQ ID NO: 145)、深绿木霉jgi|Triat2|188756(SEQ ID NO:146)、绿木霉 jgi|TriviGv29_8_2|217176(SEQ IDNO:147)、烟曲霉gi|70993168(SEQ ID NO:148)、米曲霉gi|169776800(SEQ ID NO:149)、黑曲霉gi|145236399 (SEQ ID NO:150)、棒曲霉gi|121708799(SEQ ID NO:151)、黑曲霉 gi|145239871(SEQ ID NO:152)、棒曲霉gi|121714541(SEQ ID NO:153)、土 曲霉gi|115387645(SEQ ID NO:154)、烟曲霉gi|70982015(SEQ ID NO:155) 、核盘菌gi|156045898(SEQ ID NO:156)、灰葡萄孢霉gi|154321758(SEQ ID NO:157)、费希新萨托菌gi|119499774(SEQ ID NO:158)、柄篮状菌 gi|242798348(SEQ ID NO:159)、马尼弗青霉菌gi|212541546(SEQ ID NO: 160)、玉米赤霉gi|46114460(SEQ ID NO:161)、尖孢镰刀菌gi|342890694 (SEQ ID NO:162)、Grosmannia clavigera gi|320592937(SEQ ID NO:163)、黑白轮枝菌gi|302406186(SEQ ID NO:164)、大丽轮枝菌gi|346971444(SEQ ID NO:165)、烟曲霉CAE51075.1(SEQ ID NO:556)、米曲霉XP_001820835.1 (SEQ ID NO:557)、产黄青霉XP_002564029.1(SEQ ID NO:558)、构巢曲霉 XP_664805.1(SEQ ID NO:559)、产黄青霉XP_002565814.1(SEQ ID NO: 560)、嗜热毁丝霉XP_003663689.1(SEQ ID NO:561)、粗糙链孢霉 XP_958412.1(SEQ ID NO:562)、黑曲霉XP_001394118.1(SEQ ID NO:563) 、烟曲霉CAE17674.1(SEQ ID NO:564)、黑曲霉XP_001400873.1(SEQ ID NO:565)、烟曲霉CAE46473.1(SEQ ID NO:566)、米曲霉XP_002373530.1 (SEQ ID NO:567)、构巢曲霉XP_660624.1(SEQ ID NO:568)、产黄青霉 XP_002562943.1(SEQ ID NO:569)、烟曲霉CAE17675.1(SEQ ID NO:570)、 烟曲霉EAL86850.2(SEQ ID NO:571)、粗糙链孢霉XP_961957.1(SEQ ID NO:572)、米曲霉BAB97387.1(SEQ ID NO:573)及其同源物。
因此，在某些实施方式中，本公开的蛋白酶(通常tpp1蛋白酶)的氨基 酸序列与选自SEQ ID NO:145-165、SEQ ID NO:556-573的氨基酸序列具有 50％或更高的同一性(例如，60％、65％、70％、75％、80％、85％、90％、 91％、92％、93％、94％、95％、96％、97％、98％、99％、99.5％或更高)。在 一些实施方式中，蛋白酶与选自SEQ ID NO:145-165、SEQID NO:556-573 的氨基酸序列具有100％的同一性。
在一些实施方式中，tpp1是里氏木霉tpp1。里氏木霉tpp1编码的氨基酸 序列示于SEQ ID NO:145.中。在其它实施方式中，本公开的蛋白酶的氨基 酸序列与SEQ ID NO:145具有50％或更高的同一性(例如，60％、65％、70％、 75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、 99％、99.5％或更高)。在进一步的实施方式中，蛋白酶与SEQ ID NO:145具 有100％的同一性。
同源蛋白酶
本公开的其它实施方式涉及降低与本公开的蛋白酶同源的蛋白酶的活 性。本文中使用的“同源性”是指参比序列与第二序列的至少一个片段之间的 序列相似性。同源物可以通过本领域中已知的任何方法确定，优选通过使用 BLAST工具将参比序列与单一第二序列或序列的片段比较或与序列的数据 库比较确定。如下面描述的，BLAST基于序列同一性和相似性比较序列。
在两个或更多个核酸或氨基酸序列的情况中，术语“相同”或百分“同一 性”是指相同的两个或更多个序列或子序列。当在比较窗或指定区域上进行比 较或比对以获得最大对应性时，如使用以下序列比较算法之一或通过人工比 对和目视检查测量的，如果两个序列具有指定百分比的氨基酸残基或核苷酸 相同(即，在指定区域上或在未指明的情况下在整个序列上29％的同一性， 任选地30％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、 90％、95％、99％或100％的同一性)，则两个序列“基本上相同”。任选地，同 一性在长度至少约50个核苷酸(或10个氨基酸)的区域上存在，或更优选 地在长度100-500或1000或更多个核苷酸(或20、50、200或更多个氨基酸) 的区域上存在。
对于序列比较，通常一个序列用作将测试序列与其对比的参比序列。当 使用序列比较算法时，测试序列和参比序列输入计算机中，指定子序列坐标 (如果必要)并指定序列算法程序参数。可以使用缺省程序参数，或者可以 指定可选的参数。然后序列比较算法基于序列参数计算测试序列相对于参比 序列的百分序列同一性。当比较两个序列的同一性时，序列不必须是连续的， 但任何空位将带来降低总体百分同一性的罚分。对于blastn，缺省参数是Gap 开放罚分＝5和Gap延伸罚分＝2。对于blastp，缺省参数是Gap开放罚分＝11和Gap延伸罚分＝1。
如本文中使用的，“比较窗”包括指任何数目的连续位置的片段，包括， 但不限于20-600，通常约50-约200，更通常约100-约150，其中在两个序列 最佳对准后，序列可以与具有相同数目的连续位置的参比序列比较。用于比 较的序列比对方法是本领域中公知的。可以进行用于比较的序列的最佳比对， 例如通过Smith和Waterman(1981)的局部同源性算法、通过Needleman和 Wunsch(1970)J Mol Biol 48(3):443-453的同源性比对算法、通过Pearson和 Lipman(1988)Proc Natl Acad Sci USA 85(8):2444-2448的相似性检索方法、通 过这些算法的计算机实施(Wisconsin Genetics Software Package,GeneticsComputer Group,575Science Dr.,Madison,WI中的GAP、BESTFIT、FASTA 和TFASTA)或通过人工比对和目视检查[参见，例如Brent等,(2003)Current Protocols in MolecularBiology,John Wiley&Sons,Inc.(Ringbou Ed)]。
适合于确定百分序列同一性和序列相似性的算法的两个实例是BLAST 和BLAST2.0算法，其分别描述于Altschul等(1997)Nucleic Acids Res 25(17): 3389-3402和Altschul等(1990)J.Mol Biol 215(3)：403-410中。用于进行 BLAST分析的软件通过National Center for Biotechnology Information公开可 得。该算法包括通过鉴别查询序列中长度W的短字串，其在与数据库序列中 相同长度的字串比对时匹配或满足一定的正值阈值评分T，由此首先鉴别高 分值序列对(HSP)。T被称为邻近字串评分阈值(Altschul等，同上)。这些初 始邻近字串选中充当启动检索的种子以查找包含其的更长的HSP。字串选中 在两个方向上沿着每个序列延伸，直到累积的比对分值可增加为止。对于核 苷酸序列，利用参数M(一对匹配残基的奖励分数，通常＞0)和N(非配对残基 的罚分，通常＜0)计算累积分数。对于氨基酸序列，使用评分矩阵计算累积 分数。每个方向上字串选中的延伸在下列情况时停止：累积比对分值由其最 大实现值跌落量X时；累积分值由于累积了一个或多个负分残基比对而达到 0以下时；或达到任一个序列的末端时。BLAST算法参数W、T和X确定 比对的灵敏度的速度。BLAST程序(对于核苷酸序列)使用的默认字长(W)为 11，期望值(E)为10，M＝5，N＝-4和两条链的比较。对于氨基酸序列，BLAST 程序使用的默认字长(W)为3，期望值(E)为10和BLOSUM62评分矩阵[参见 Henikoff和Henikoff,(1992)Proc NatlAcad Sci USA 89(22):10915-10919]比对 值(B)50，期望值(E)10,M＝5,N＝-4和两条链的比较。
BLAST算法还进行两个序列之间相似性的统计分析(参见，例如Karlin 和Altschul，(1993)Proc.Natl.Acad.Sci.USA 90(12)：5873-5787)。由BLAST 算法提供的一种相似性测量是最小和概率(P(N))，其提供了两个核苷酸序列或 氨基酸序列之间偶然发生匹配的概率的指示。例如，如果测试核酸与参比核 酸的对比中的最小和概率低于约0.2，更优选低于约0.01，最优选低于约 0.001，则认为该核酸与参比序列相似。
除了如上所述的序列同一性百分比之外，两个核酸序列或多肽基本上相 同的另一指示是由第一核酸编码的多肽与针对由第二核酸编码的多肽产生的 抗体具有免疫交叉反应性，如下所述。因此，例如在两个肽仅存在保守置换 的差异时，多肽与第二多肽通常基本上相同。两个核酸序列基本上相同的另 一指示是两个分子或它们的互补序列在严格条件下彼此杂交，如下所述。两 个核酸序列基本上相同的再另一指示是相同的引物可用于扩增该序列。
如本文中公开的，本公开的蛋白酶也可以包括作为由如上所述的蛋白酶 基因编码的保守修饰蛋白酶变体的蛋白酶。如本文所用的，“保守修饰变体” 包括编码的氨基酸序列的单个置换、删除或添加，其导致氨基酸被化学相似 的氨基酸置换。提供功能上相似的氨基酸的保守置换表是本领域中公知的。 这类保守修饰变体附加于多态性变体、物种间同源物和本公开的等位基因且 不排除它们。以下八个组包含相互为保守置换的氨基酸：1)丙氨酸(A)、甘 氨酸(G)；2)天冬氨酸(D)、谷氨酸(E)；3)天冬酰胺(N)、谷氨酰胺(Q)；4)精 氨酸(R)、赖氨酸(K)；5)异亮氨酸(I)、亮氨酸(L)、蛋氨酸(M)、缬氨酸(V)； 6)苯丙氨酸(F)、酪氨酸(Y)、色氨酸(W)；7)丝氨酸(S)、苏氨酸(T)和8)半 胱氨酸(C)、蛋氨酸(M)(参见，例如Creighton,Proteins(1984))。
图45-48描绘了所选择的丝状真菌的天冬氨酸蛋白酶、枯草溶菌素蛋白 酶、谷氨酸蛋白酶和Sedolisin蛋白酶的系统发生树。
降低本发明蛋白酶的活性的方法
本公开的进一步的方面涉及降低表达异源多肽如哺乳动物多肽的丝状真 菌细胞中存在的蛋白酶的活性。
丝状真菌细胞中存在的蛋白酶的活性可以通过本领域技术人员已知的任 何方法降低。
在一些实施方式中，降低的蛋白酶活性通过降低蛋白酶的表达实现，例 如通过启动子修饰或RNAi。
在其它实施方式中，降低的蛋白酶活性通过修饰编码蛋白酶的基因实现。 这类修饰的实例包括，但不限于敲除突变、截断突变、点突变、错义突变、 置换突变、移码突变、插入突变、重复突变、扩增突变、易位突变或反转突 变，且导致相应蛋白酶活性的降低。在目标蛋白酶编码基因中产生至少一个 突变的方法是本领域中公知的，且包括，但不限于随机诱变和筛选、定点诱 变、PCR诱变、插入诱变、化学诱变和照射。
在某些实施方式中，蛋白酶编码基因的一部分被修饰，如编码催化结构 域的区域、编码区域或编码区域的表达需要的控制序列。基因的这种控制序 列可以是启动子序列或其功能性部分，即足以影响基因的表达的部分。例如， 启动子序列可以灭活从而导致无表达，或者较弱启动子可以替代原始启动子 序列以减少编码序列的表达。用于可能的修饰的其它控制序列包括，但不限 于前导序列、前肽序列、信号序列、转录终止子和转录激活子。
存在于表达重组多肽的丝状真菌细胞中的本公开的蛋白酶编码基因也可 以通过利用基因删除技术修饰以消除或降低该基因的表达。基因删除技术使 得基因的部分或完全删除成为可能，从而消除其表达。在这类方法中，基因 的删除可以使用构建为连续地包含该基因侧翼的5'和3'区域的质粒通过同源 重组完成。
存在于表达重组多肽的丝状真菌细胞中的本公开的蛋白酶编码基因也可 以通过引入、替换和/或移除基因中的一个或多个核苷酸或者该基因的转录或 翻译所需要的其控制序列来修饰。例如，核苷酸可以插入或移除以引入终止 密码子、移除起始密码子或开放阅读框的移码。这样的修饰可以通过本领域 中已知的方法完成，包括，但不限于定点诱变和peR产生的诱变(参见，例如， Botstein和Shortie,1985,Science 229:4719；Lo等,1985,Proceedings of the National Academy of Sciences USA 81:2285；Higuchi等,1988,Nucleic Acids Research 16:7351；Shimada,1996,Meth.Mol.Biol.57:157；Ho等,1989,Gene 77:61；Horton等,1989,Gene 77:61及Sarkar和Sommer,1990,BioTechniques 8:404)。
另外，存在于表达重组多肽的丝状真菌细胞中的本公开的蛋白酶编码基 因可以通过将基因插入包含与该基因同源的核酸片段的破坏性核酸构建体中 (其将产生同源性区域的重复和在重复的区域之间并入构建体DNA)利用基 因破坏技术修饰。如果插入的构建体将基因的启动子和编码序列分隔开或干 扰编码序列以使得产生非功能性基因产物，则这种基因破坏可以消除基因表 达。破坏构建体可以简单地是伴随与该基因同源的5'和3'区域的选择标记基 因。选择标记使得能够鉴别包含破坏的基因的转化体。
存在于表达重组多肽的丝状真菌细胞中的本公开的蛋白酶编码基因也可 以通过基因转化的方法修饰(参见，例如，Iglesias和Trautner,1983,Molecular GeneralGenetics 189:5 73-76)。例如，在基因转化中，对应于基因的核苷酸序 列体外诱变以产生缺陷核苷酸序列，其随后转化到木霉属菌株中以产生缺陷 基因。通过同源重组，缺陷核苷酸序列替代内源基因。可能希望的是缺陷核 苷酸序列也包含用于选择含缺陷基因的转化体的标记。
存在于表达重组多肽的丝状真菌细胞中的本公开的蛋白酶编码基因也可 以使用与基因的核苷酸序列互补的核苷酸序列通过已建立的反义技术修饰( 参见，例如，Parish和Stoker,1997,FEMS Microbiology Letters 154:151-157)。 特别地，丝状真菌细胞的基因表达可以通过引入与基因的核苷酸序列互补的 核苷酸序列(其可以转录到菌株中且能够与细胞中产生的mRNA杂交)降低 或灭活。在允许互补的反义核苷酸序列与mRNA杂交的条件下，翻译的蛋白 质的量因此降低或消除。
另外，存在于表达重组多肽的丝状真菌细胞中的本公开的蛋白酶编码基 因也可以通过已建立的RNA干扰(RNAi)技术修饰(参见，例如，WO 2005/056772和WO 2008/080017)。
存在于表达重组多肽的丝状真菌细胞中的本公开的蛋白酶编码基因也可 以使用本领域中公知的方法通过随机或特异性诱变修饰，其包括，但不限于 化学诱变(参见，例如，Hopwood,The Isolation of Mutants in Methods in Microbiology(J.R.Norris和D.W.Ribbons编辑)pp.363-433,Academic Press, New York,25 1970)。基因修饰可以通过使丝状真菌细胞经历诱变和筛选其中 基因表达降低或灭活的突变体细胞来进行。例如，诱变(其可以是特异性的 或随机的)可以通过使用合适的物理和化学诱变剂、使用合适的寡核苷酸、 使DNA序列经历peR产生的诱变或其任何组合进行。物理和化学诱变剂的 实例包括，但不限于紫外(UV)照射、羟胺、N-甲基-N'-硝基-N-亚硝基胍 (MNNG)、N-甲基-N'-亚硝基胍(NTG)、O-甲基羟胺、亚硝酸、甲磺酸乙酯 (EMS)、亚硫酸氢钠、甲酸和核苷酸类似物。当使用这类试剂时，诱变通常 通过在所选择的诱变剂存在下在合适的条件下孵育待诱变的木霉属细胞，且 随后选择表现出降低的或没有基因表达的突变体来进行。
在某些实施方式中，本公开的蛋白酶编码基因中的至少一个突变或修饰 导致不具有可检测的蛋白酶活性的修饰的蛋白酶。在其它实施方式中，本公 开的蛋白酶编码基因中的至少一个修饰导致与对应非修饰蛋白酶相比具有至 少低25％、低至少50％、低至少75％、低至少90％、至少95％、至少100％、 至少200％、至少300％、至少400％、至少500％、至少600％、至少700％、 至少800％、至少900％、至少1,000％或低更高百分比的蛋白酶活性的修饰的 蛋白酶。
在某些实施方式中，例如，在木霉属细胞中，本公开的蛋白酶编码基因 中的至少一个突变或修饰导致总蛋白酶活性降低到对应亲本木霉属细胞的总 蛋白酶活性的49％或更低(通常具有至少两个不同蛋白酶基因中的突变)，或 降低到31％或更低(通常具有至少三个不同蛋白酶基因中的突变)，或降低到 13％或更低(通常具有至少四个不同蛋白酶基因中的突变)，或降低到10％或 更低(通常具有至少五个不同蛋白酶基因中的突变)，或降低到6.3％或更低 (通常具有至少六个不同蛋白酶基因中的突变)，或降低到5.5％或更低(通 常具有至少七个不同蛋白酶基因中的突变)。
本发明的异源多肽
本文的本发明进一步涉及通过降低细胞中存在的蛋白酶的活性提高表达 异源多肽的丝状真菌细胞中该异源多肽的产生。
如本文中使用的，“异源多肽”是指非天然存在于本公开的丝状真菌细胞 中(即丝状真菌细胞内源)的或在丝状真菌细胞中与多肽的内源形式相比以 提高的水平表达的多肽。在某些实施方式中，异源多肽是哺乳动物多肽。在 其它实施方式中，异源多肽是非哺乳动物多肽。
哺乳动物多肽
本公开的哺乳动物多肽可以是具有目标生物活性的任何哺乳动物多肽。 如本文中使用的，“哺乳动物多肽”是在哺乳动物中天然表达的多肽、源自哺 乳动物中天然表达的多肽的多肽或其片段。哺乳动物多肽还包括保持生物活 性的肽和寡肽。本公开的哺乳动物多肽还可以包括结合以形成编码的产物的 两个或更多个多肽。本公开的哺乳动物多肽可以进一步包括融合多肽，其包 含从至少两个不同多肽获得的部分或完整氨基酸序列的组合。哺乳动物多肽 还可以包括任何所公开的哺乳动物多肽的天然存在等位的和工程化的变体及 杂合哺乳动物多肽。
哺乳动物多肽可以是天然糖基化的多肽或天然非糖基化的多肽。
合适的哺乳动物多肽的实例包括，但不限于免疫球蛋白、抗体、抗原、 抗微生物肽、酶、生长因子、激素、干扰素、细胞因子、白介素、免疫增强 剂(immunodilator)、神经递质、受体、报告蛋白、结构蛋白和转录因子。
合适的哺乳动物多肽的特定实例包括，但不限于免疫球蛋白、免疫球蛋 白重链、免疫球蛋白轻链、单克隆抗体、杂合抗体、F(ab')2抗体片段、F(ab) 抗体片段、Fv分子、单链Fv抗体、二聚化抗体片段、三聚化抗体片段、功 能性抗体片段、免疫粘附素、胰岛素样生长因子1、生长激素、胰岛素、干 扰素α2b、成纤维细胞生长细胞21、人血清白蛋白、骆驼抗体和/或抗体片段、 单域抗体、多聚单域抗体和促红细胞生成素。
合适的哺乳动物多肽的其它实例包括，但不限于氧化还原酶、转移酶、 水解酶、裂合酶、异构酶、连接酶、氨肽酶、淀粉酶、糖酶、羧肽酶、过氧 化氢酶、糖基转移酶、脱氧核糖核酸酶、酯酶、半乳糖苷酶、β-半乳糖苷酶、 葡糖苷酶、葡糖醛酸酶、葡糖醛酸酯酶、卤代过氧化物酶、转化酶、脂肪酶、 氧化酶、磷脂酶、蛋白水解酶、核糖核酸酶、尿激酶、白蛋白、胶原蛋白、 弹性蛋白原和弹性蛋白。
非哺乳动物多肽
本公开的非哺乳动物多肽可以是具有目标生物活性的任何非哺乳动物多 肽。如本文中使用的，“非哺乳动物多肽”是非哺乳动物生物体如真菌细胞中 天然表达的多肽、源自非哺乳动物生物体中天然表达的多肽的多肽或其片段。 非哺乳动物多肽还包括保持生物活性的肽和寡肽。本公开的非哺乳动物多肽 还可以包括结合以形成编码的产物的两个或更多个多肽。本公开的非哺乳动 物多肽可以进一步包括融合多肽，其包含从至少两个不同多肽获得的部分或 完整氨基酸序列的组合。非哺乳动物多肽还可以包括任何所公开的非哺乳动 物多肽的天然存在的等位的和工程化的变体及杂合非哺乳动物多肽。
合适的非哺乳动物多肽的实例包括，但不限于氨肽酶、淀粉酶、糖酶、 羧肽酶、过氧化氢酶、纤维素酶、几丁质酶、角质酶、脱氧核糖核酸酶、酯 酶、α-半乳糖苷酶、β-半乳糖苷酶、葡糖淀粉酶、α-葡糖苷酶、β-葡糖苷酶、 转化酶、漆酶、脂肪酶、变构酶(mutanase)、氧化酶、果胶分解酶、过氧化物 酶、磷脂酶、肌醇六磷酸酶、多酚氧化酶、蛋白水解酶、核糖核酸酶、转谷 氨酰胺酶和木聚糖酶。
异源多肽的产生
目标异源多肽使用本领域中已知的方法通过在用于产生异源多肽的营养 培养基中培养包含至少三种具有降低的活性的蛋白酶的本公开丝状真菌细胞 而由该细胞产生。例如，细胞可以通过摇瓶培养、实验室中的小规模或大规 模发酵(包括连续、分批、分批补料或固态发酵)或在合适培养基中和允许 表达和/或分离多肽的条件下进行的工业化发酵进行培养。培养使用本领域中 已知的方法发生在包含碳和氮源及无机盐的合适营养培养基中。合适的培养 基可从供应商获得或可以按照公布的组成(例如，在美国典型培养物培养中 心的目录中)制备。分泌的多肽可以从培养基直接回收。如果多肽不分泌， 它可以从细胞裂解产物获得。
由包含至少三种具有降低的活性的蛋白酶的本公开丝状真菌细胞产生的 目标异源多肽可以使用对于异源多肽特异性的本领域中已知的方法检测。这 些检测方法可以包括，但不限于使用特异性抗体、高效液相色谱、毛细管色 谱、酶产物的形成、酶底物的消失和SDS-PAGE。例如，可以使用酶分析测 定酶的活性。用于测定多种酶的酶活性的过程是本领域中已知的(参见，例如， O.Schomburg和M.Salzmann(编辑),Enzyme Handbook,Springer-Verlag,New York,1990)。
所得的异源多肽可以通过本领域中已知的方法分离。例如，目标异源多 肽可以通过常规方法从培养基分离，所述方法包括，但不限于离心、过滤、 萃取、喷雾干燥、蒸发和沉淀。分离的异源多肽然后可以进一步通过本领域 中已知的多种过程纯化，其包括，但不限于色谱法(例如，离子交换色谱、亲 和色谱、疏水性色谱、层析聚焦色谱和尺寸排阻色谱)、电泳过程(例如，制 备型等电聚焦(IEF)、差异溶解性(例如，硫酸铵沉淀)或萃取(参见，例如， Protein Purification,J.-C.Janson和Lars Ryden编辑,VCH Publishers,New York,1989)。
编码异源多肽的多核苷酸的制备
本公开的异源多核苷酸的序列通过本领域中已知的任何合适的方法制 备，其包括，但不限于直接化学合成或克隆。对于直接化学合成，核酸多聚 物的形成通常涉及3'-封闭和5'-封闭的核苷酸单体顺序添加到生长的核苷酸 链的末端5'-羟基上，其中各次添加通过生长链的末端5'-羟基对添加的单体 (其通常是磷衍生物如磷酸三酯、亚磷酰胺等)的3'位的亲核攻击实现。这 种方法是本领域技术人员已知的且在相关文章和文献中描述[例如，Matteucci 等,(1980)Tetrahedron Lett 21:719-722；美国专利No.4,500,707、5,436,327和 5,700,637]。另外，所需的序列可以通过使用合适的限制性酶分裂DNA、使 用凝胶电泳分离片段和之后经由本领域技术人员中已知的技术如利用聚合酶 链反应(PCR；例如美国专利No.4,683,195)从凝胶回收所需的核酸序列而从 天然来源分离。
本公开的各异源多核苷酸可以并入表达载体中。“表达载体”或“载体”是 指转导、转化或感染宿主细胞的化合物和/或组合物，从而使得细胞表达该细 胞本来的核酸和/或蛋白质以外的核酸和/或蛋白质，或以非该细胞原始的方式 表达核酸和/或蛋白质。“表达载体”包含宿主细胞待表达的核酸(一般地是 RNA或DNA)的序列。任选地，表达载体也包括有助于核酸进入宿主细胞 的物质，如病毒、脂质体、蛋白质衣壳等。设想用于本公开中的表达载体包 括核酸序列可以插入其中的与任何优选的或需要的操作元件一起的核酸序 列。此外，表达载体必须是可以转移到宿主细胞中并在其中复制的表达载体。 优选的表达载体是质粒，特别是具有已良好证明的限制性位点并包含核酸序 列的翻译优选的或所需的操作元件的质粒。这类质粒以及其它表达载体是本 领域中公知的。
单个多核苷酸的并入可以通过已知的方法完成，其包括，例如，使用限 制性酶(如BamHI、EcoRI、HhaI、XhoI、XmaI等)切割表达载体(例如质 粒)中的特定位点。限制性酶产生单链末端，该单链末端可以与具有与切割 的表达载体的末端互补的末端序列或合成为具有序列与切割的表达载体的末 端互补的末端序列的多核苷酸退火。退火使用适宜的酶(例如，DNA连接酶) 进行。如本领域技术人员理解的，表达载体和所需的多核苷酸两者经常用相 同的限制性酶切割，从而确保表达载体的末端和多核苷酸的末端彼此互补。 另外，DNA连接体可以用于帮助将核酸序列连接到表达载体中。
一系列的单个多核苷酸也可以通过利用本领域中已知的方法(例如，美 国专利No.4,683,195)进行组合。
例如，所需的多核苷酸开始可以各自在单独的PCR中产生。之后，设计 特异性引物以使得PCR产物的末端包含互补序列。当PCR产物混合、变性 和再退火时，在其3’末端具有匹配序列的链重叠并可以起到彼此的引物的作 用。这种重叠通过DNA聚合酶延伸以产生其中原始序列被“剪接”到一起的分 子。以这种方式，一系列的单个多核苷酸可以“剪接”到一起并随后同时转导 到宿主细胞中。因此，实现多个多核苷酸中各多核苷酸的表达。
单个多核苷酸或“剪接”的多核苷酸然后并入表达载体中。本公开对于多 核苷酸并入表达载体中的方法没有限制。本领域普通技术人员熟悉用于将多 核苷酸并入表达载体中的必要步骤。典型的表达载体包含需要的多核苷酸， 在其之前是一个或多个调控区域以及核糖体结合位点，例如大肠杆菌中的长 度3-9个核苷酸并位于起始密码子上游3-11个核苷酸的核苷酸序列。参见 Shine和Dalgarno(1975)Nature 254(5495):34-38及Steitz(1979)Biological Regulation and Development(编者Goldberger,R.F.),1:349-399(Plenum,New York)。
如本文中使用的，术语“可操作地连接”是指其中控制序列相对于DNA序 列或多核苷酸的编码序列处于适宜的位置以使得控制序列指导多肽的表达。
调控区域包括例如，包含启动子和操纵基因的那些区域。启动子与所需 的多核苷酸可操作地连接，从而通过RNA聚合酶启动多核苷酸的转录。操纵 基因是与启动子相邻的核酸序列，其包含其中阻遏蛋白可以结合的蛋白质结 合结构域。在不存在阻遏蛋白的情况下，转录通过启动子启动。当存在阻遏 蛋白时，对于操纵基因的蛋白质结合结构域特异性的阻遏蛋白结合操纵基因， 从而抑制转录。以这种方式，转录的控制基于所使用的特定调控区域和相应 阻遏蛋白的存在或不存在完成。实例包括乳糖启动子(Lad阻遏蛋白在与乳糖 接触时改变构象，从而阻止Lad阻遏蛋白结合操纵基因)和色氨酸启动子(当 与色氨酸复合时，TrpR阻遏蛋白具有结合操纵基因的构象；在不存在色氨酸 的情况下，TrpR阻遏蛋白具有不结合操纵基因的构象)。另一个实例是tac启 动子(参见de Boer等,(1983)ProcNatl Acad Sci USA 80(l):21-25)。如本领域普 通技术人员理解的，这些和其它表达载体可以用于本公开中，且本公开在这 一方面不受限制。
虽然任何合适的表达载体可以用于并入所需的序列，但容易获得的表达 载体包括，但不限于：质粒如pSClOl、pBR322、pBBRlMCS-3、pUR、pEX、 pMR100、pCR4、pBAD24、pUC19、pRS426和噬菌体如M1 3噬菌体和λ噬 菌体，当然，这类表达载体可能仅适合于特定宿主细胞。但是，本领域技术 人员可以容易地通过常规试验确定是否任何特定表达载体适合于任何给定宿 主细胞。例如，表达载体可以引入宿主细胞中，然后监测宿主细胞的存活力 和载体中包含的序列的表达。另外，可以参考描述表达载体及其对任何特定 宿主细胞的适合性的相关文章和文献。
用于本发明目的(包括所需异源多肽、酶和一种或多种本文描述的催化 结构域的表达)的合适的表达载体包括包含与组成型或诱导型启动子可操作 地连接的编码所需异源多肽、酶或催化结构域的多核苷酸的表达载体。用于 与这类多核苷酸可操作地连接的特别适合的启动子的实例包括来自以下基因 的启动子：gpdA、cbh1、米曲霉TAKA淀粉酶、米赫根毛霉(Rhizomucor miehei) 天冬氨酸蛋白酶、黑曲霉中性α-淀粉酶、黑曲霉酸性稳定α-淀粉酶、黑曲霉 葡糖淀粉酶(glaA)、泡盛曲霉(Aspergillus awamori)glaA、米赫根毛霉脂肪酶、 米曲霉碱性蛋白酶、米曲霉磷酸丙糖异构酶、构巢曲霉乙酰胺酶、米曲霉乙 酰胺酶、尖孢镰刀菌胰蛋白酶样蛋白酶、真菌内α-L-阿拉伯糖酶(abnA)、真 菌α-L-阿拉伯呋喃糖酶A(abfA)、真菌α-L-阿拉伯呋喃糖酶B(abfB)、真菌 木聚糖酶(xlnA)、真菌肌醇六磷酸酶、真菌ATP-合酶、真菌亚基9(oliC)、真 菌磷酸丙糖异构酶(tpi)、真菌醇脱氢酶(adhA)、真菌α-淀粉酶(amy)、真菌淀 粉葡糖苷酶(glaA)、真菌乙酰胺酶(amdS)、真菌甘油醛-3-磷酸脱氢酶(gpd)、 酵母醇脱氢酶、酵母乳糖酶、酵母3-磷酸甘油酸激酶、酵母磷酸丙糖异构酶、 细菌α-淀粉酶、细菌Spo2和SSO。这种合适的表达载体和启动子的实例也 描述于PCT/EP2011/070956中，其全部内容在此通过引入并入本文。
包含通过本发明的丝状真菌细胞产生的异源多肽的药物组合物
在另一个方面，本发明提供包含与药物可接受的载体一起配制的本公开 的丝状真菌细胞产生的一种或多种目标异源多肽如哺乳动物多肽的组合物， 例如药物组合物，该丝状真菌细胞具有至少三种降低的蛋白酶的活性且进一 步包含编码异源多肽的重组多核苷酸。本发明的药物组合物也可以在组合疗 法中施用，即与其它的药剂组合。例如，组合疗法可以包括与至少一种另外 的治疗剂组合的目标哺乳动物多肽。
如本文中使用的，“药物可接受的载体”包括生理相容的任何和所有的溶 剂、分散介质、包衣、抗细菌剂和抗真菌剂、等渗剂和吸收延迟剂等等。优 选地，载体适合于静脉内、肌肉内、皮下、肠胃外、脊髓或表皮施用(例如， 通过注射或输注)。取决于施用途径，活性成分，即目标哺乳动物多肽，可以 包被在材料中以保护化合物免于酸和可灭活该化合物的其它自然条件的作 用。
本发明的药物组合物可以包括一种或多种药物可接受的盐。“药物可接受 的盐”是指保持母体化合物的所需生物活性且不施加任何不希望的毒理学效 应的盐(参见，例如，Berge,S.M.等(1977)J.Pharm.Sci.66:1-19)。这类盐的 实例包括酸加成盐和碱加成盐。酸加成盐包括由非毒性无机酸如盐酸、硝酸、 磷酸，硫酸、氢溴酸、氢碘酸、亚磷酸等衍生的那些，以及由非毒性有机酸 如脂族单-和二羧酸、苯基取代的链烷酸、羟基链烷酸、芳香酸、脂族和芳族 磺酸等衍生的那些。碱加成盐包括由碱土金属如钠、钾、镁、钙等衍生的那 些，以及由非毒性有机胺如N,N'-二苄基乙二胺、N-甲基葡糖胺、氯普鲁卡因、 胆碱、二乙醇胺、乙二胺、普鲁卡因等衍生的那些。
发明的药物组合物也可以包括药物可接受的抗氧化剂。药物可接受的抗 氧化剂的实例包括：(1)水溶性抗氧化剂如抗坏血酸、盐酸半胱氨酸、硫酸 氢钠、焦亚硫酸钠、亚硫酸钠等；(2)油溶性抗氧化剂如抗坏血酸棕榈酸盐、 丁羟基茴香醚(BHA)、丁基羟基甲苯(BHT)、卵磷脂、没食子酸丙酯、α-生育 酚等；和(3)金属螯合剂如柠檬酸、乙二胺四乙酸(EDTA)、山梨糖醇、酒石 酸、磷酸等。
可以用于发明的药物组合物中的合适的水性和非水性载体的实例包括 水、乙醇、多元醇(如甘油、丙二醇、聚乙二醇等)及其合适的混合物、植 物油如橄榄油及可注射有机酯如油酸乙酯。适当的流动性可例如通过使用包 衣材料如卵磷脂、通过保持所需的颗粒尺寸(在分散体的情况中)和通过使 用表面活性剂维持。
这些组合物也可以包含辅剂如防腐剂、湿润剂、乳化剂和分散剂。防止 微生物的存在可以通过消毒过程和通过包括各种抗细菌剂和抗真菌剂(例如， 对羟基苯甲酸酯、氯丁醇、苯酚、山梨酸等)来确保。也可能需要的是包括 等渗剂如糖、氯化钠等到组合物中。另外，注射药物形式的延长吸收可通过 包括延迟吸收的试剂如单硬脂酸铝和明胶获得。
药物可接受的载体包括无菌水性溶液或分散液和用于即时制备无菌注射 溶液或分散液的无菌粉末。用于药物活性物质的这类介质和试剂的使用是本 领域中已知的。除非任何常规的介质或试剂与活性化合物不相容，其在本发 明的药物组合物中的使用是可预期的。补充的活性化合物也可以合并到组合 物中。
治疗组合物通常必须在制造和储存条件下是无菌的和稳定的。组合物可 以配制为溶液、微乳液、脂质体或其它适合于高药物浓度的有序结构。载体 可以是包含例如水、乙醇、多元醇(如甘油、丙二醇和液体聚乙二醇等)及 其合适的混合物的溶剂或分散介质。适当的流动性可例如通过使用包衣材料 如卵磷脂、通过保持所需的颗粒尺寸(在分散体的情况中)和通过使用表面 活性剂维持。在许多情况中，优选的是组合物中包括等渗剂，例如糖、多元 醇如甘露醇、山梨醇或氯化钠。可注射组合物的延长吸收可通过在组合物中 包括延迟吸收的试剂例如单硬脂酸盐和明胶获得。
可以如下制备无菌注射溶液：根据需要在具有上文所列举的一种组分或 组分组合的合适溶剂中掺入需要量的活性化合物，接着进行无菌微滤。一般 而言，通过将活性化合物掺入无菌媒介中来制备分散体，所述无菌媒介含有 基础分散介质及所需的那些上文所列举的组分中的其它组分。在用于制备无 菌注射溶液的无菌粉末的情况中，特定制备方法是自先前无菌过滤的溶液产 生活性成分加任何其它需要的成分的粉末的真空干燥和冷冻干燥(冻干)。
可以与载体材料组合以产生单一剂型的活性成分的量随待治疗的受试者 和特定的施用方式变化。可以与载体材料组合以产生单一剂型的活性成分的 量一般是产生治疗效果的该组合物的量。一般地，在百分之一百中，这一量 在约0.01％-约99％的活性成分的范围内，优选约0.1％-约70％，最优选与药 物可接受的载体组合的约1％-约30％的活性成分。
剂量方案经调整以提供最佳的所需反应(例如，治疗反应)。例如，可以 施用单一的大剂量，可以随时间施用几个分开的剂量或可以如治疗状况的紧 急性所指示的成比例地降低或提高剂量。特别有利的是配制单位剂型的肠胃 外组合物以实现施用方便性和剂量均匀性。如本文中使用的，单位剂型是指 适合作为用于待治疗受试者的单一剂量的物理离散单元；各单元包含与需要 的药物载体结合的经计算以产生所需治疗效果的预定量的活性化合物。本发 明的单位剂型的规格由以下方面决定并直接取决于以下方面：(a)活性化合物 的独特特征和待获得的特定治疗效果和(b)组合这种活性化合物以处理个体 的敏感性的领域中固有的限制。
对于目标哺乳动物多肽的施用，特别是其中哺乳动物多肽是抗体的情况 中，剂量范围为宿主体重的约0.0001-100mg/kg，更通常0.01-5mg/kg。例如， 剂量可以是0.3mg/kg体重，1mg/kg体重，3mg/kg体重，5mg/kg体重或10 mg/kg体重或者在1-10mg/kg的范围内。示例性的治疗方案需要每周施用一 次、每两周一次、每三周一次、每四周一次、一月一次、每3个月一次或每 3-6个月一次。用于抗体的特定剂量方案可以包括经静脉内施用的1mg/kg体重或3mg/kg体重，其中抗体使用以下给药时间表之一给予：(i)每四周六个 剂量，然后每三个月；(ii)每三周；(iii)3mg/kg体重一次，之后每三周1mg/kg 体重。
或者，目标哺乳动物多肽可以作为持续释放制剂施用，在该情况中需要 较低频率的施用。剂量和频率根据施用的物质在患者中的半衰期变化。一般 地，人抗体显示最长的半衰期，之后是人源化抗体、嵌合抗体和非人抗体。 施用剂量和频率可以根据治疗是预防性的还是治疗性的而变化。在预防性应 用中，相对低的剂量在长时间段中以相对较低的频率施用。一些患者在其剩 余生存期中持续接受治疗。在治疗性应用中，有时需要以相对短的间隔施用 相对高的剂量直到疾病进展降低或终止，且优选直到患者显示疾病症状的部分或完全改善。随后，患者可以施用预防性方案。
本公开的药物组合物中活性成分的实际剂量水平可以变化以获得对于特 定患者、组成和施用途径有效实现所需治疗反应而对患者非毒性的活性成分 量。选择的剂量水平取决于多种药代动力学因素，包括所采用的本发明特定 组合物或其酯、盐或氨基化合物的活性、施用途径、施用时间、使用的特定 化合物的排泄率、治疗持续时间、与使用的特定组合物组合的其它药物、化 合物和/或物质、所治疗患者的年龄、性别、体重、状况、总体健康和先前病 史及医学领域中公知的类似因素。
本公开的免疫球蛋白的“治疗有效剂量”优选导致疾病症状严重性的降 低、无疾病症状期的频率和持续时间的增加或者防止由于患病导致的损害或 残疾。例如，对于肿瘤的治疗，“治疗有效剂量”优选相对于未治疗受试者抑 制细胞生长或肿瘤生长至少约20％，更优选至少约40％，甚至更优选至少约 60％，且再更优选至少约80％。化合物抑制肿瘤生长的能力可以在指示人肿 瘤中的效力的动物模型系统中评估。或者，组合物的这一性质可以通过熟练 的实施者已知的分析方法检验化合物体外抑制这种抑制作用的能力来评估。治疗有效量的治疗化合物可以减小肿瘤尺寸或以其它方式改善受试者的症 状。本领域技术人员能够基于如受试者体型、受试者症状的严重性和所选择 的特定组合物或施用途径的因素确定这种量。
本公开的组合物可以使用本公开中已知的多种方法中的一种或多种经由 一种或多种施用途径施用。如本领域技术人员理解的，施用途径和/或方式根 据所需的结果变化。用于本发明的结合部分的特定施用途径包括静脉内、肌 肉内、皮内、腹膜内、皮下、脊髓或其它肠胃外施用途径，例如通过注射或 输注。如本文中使用的，短语“肠胃外施用”意思是肠施用和局部施用以外的 施用方式，通常是通过注射，且包括，但不限于静脉内、肌肉内、动脉内、 鞘内、囊内、眶内、心脏内、皮内、腹膜内、经气管、皮下、表皮下、关节 内、囊下、蛛网膜下、脊柱内、硬膜外及胸骨内注射和输注。
或者，根据本公开的哺乳动物多肽可以经由非肠胃外途径如局部、表皮 或粘膜施用途径施用，例如，鼻内、口服、阴道、直肠、舌下或局部。
活性化合物可以用保护化合物免于快速释放的载体制备，如控释制剂， 包括植入物、透皮贴片和微囊化递送系统。可以使用生物可降解的生物相容 性聚合物如乙烯乙酸乙烯酯、聚酐、聚乙醇酸、胶原、聚原酸酯和聚乳酸。 用于制备这类制剂的许多方法获得专利或一般为本领域技术人员已知(参见， 例如，Sustained and Controlled Release DrugDelivery Systems,J.R.Robinson编 辑,Marcel Dekker,Inc.,New York,1978)。
治疗组合物可以利用本领域中已知的医疗机械施用。例如，在特定实施 方式中，本发明的治疗组合物可以利用无针皮下注射装置施用，如美国专利 No.5,399,163；5,383,851；5,312,335；5,064,413；4,941,880；4,790,824或 4,596,556中公开的装置。可用于本发明中的公知植入物和模块的实例包括： 美国专利No.4,487,603，其公开了用于以受控速率分配药物的可植入微输注 泵；美国专利No.4,486,194，其公开了用于通过皮肤施用药物的治疗装置； 美国专利No.4,447,233，其公开了用于以精确输注速率递送药物的药物输注泵；美国专利No.4,447,224，其公开了用于连续药物递送的可变流量植入输 注装置；美国专利No.4,439,196，其公开了具有多腔区室的渗透性药物递送 系统和美国专利No.4,475,196，其公开了渗透性药物递送系统。
在某些实施方式中，根据本公开的哺乳动物多肽的使用是用于治疗可用 治疗性抗体治疗的任何疾病。
本发明的丝状真菌细胞
本文的本发明还涉及通过降低或消除表达异源多肽的细胞中存在的且催 化异源多肽降解的至少三种蛋白酶的活性来提高丝状真菌细胞中异源多肽如 哺乳动物多肽的产生水平。降低或消除表达异源多肽的丝状真菌细胞中存在 的蛋白酶的活性提高了表达的重组多肽的稳定性，其导致异源多肽产生水平 的提高。丝状真菌细胞中存在的蛋白酶的活性可以例如通过修饰编码蛋白酶 的基因降低。
“丝状真菌细胞”包括来自真菌和卵菌亚门的所有丝状形式的细胞(如由Hawksworth等定义的,Ainsworth and Bisby's Dictionary of The Fungi,第8版, 1995,CAB International,University Press,Cambridge,UK)。丝状真菌细胞一般 特征在于由几丁质、纤维素、葡聚糖、脱乙酰壳多糖、甘露聚糖和其它复合 多糖组成的菌丝体壁。营养性生长是通过菌丝延伸且碳分解代谢是专性需氧 的。相反，酵母如酿酒酵母的营养性生长是通过单细胞菌体的出芽和碳分解 代谢可以是发酵的。
任何丝状真菌细胞可以用于本公开中，只要它在用核酸序列转化和/或者 经修饰或突变以降低蛋白酶活性后保持存活。优选地，丝状真菌细胞不受必 要核酸序列转导、蛋白质(例如哺乳动物蛋白)的后续表达或得到的中间体 的不利影响。
合适的丝状真菌细胞的实例包括，但不限于来自支顶孢属、曲霉属、镰 孢菌属、腐质霉属、毛霉属、毁丝霉属、链孢霉属、青霉属、柱霉属、梭孢 壳属、弯颈霉属或木霉属菌株的细胞。在某些实施方式中，丝状真菌细胞来 自木霉属、支顶孢属、曲霉属、短梗霉属、隐球菌属、金孢子菌属、 Chrysosporium lucknowense、Filibasidium、链孢霉属、赤霉属、Magnaporthe、 毛霉属、毁丝霉属、漆斑菌属、新美鞭菌属(Neocallimastix)、链孢霉属、拟青霉属(Paecilomyces)、青霉属、瘤胃壶菌属(Piromyces)、裂褶菌属 (Schizophyllum)、踝节菌属(Talaromyces)、Thermoascus、梭孢壳属或弯颈霉属 菌株。
本公开的曲霉属真菌细胞可以包括，但不限于棘孢曲霉(Aspergillusaculeatus)、泡盛曲霉(Aspergillus awamori)、棒曲霉(Aspergillus clavatus)、黄 曲霉、臭曲霉(Aspergillus foetidus)、烟曲霉(Aspergillus fumigatus)、日本曲霉(Aspergillus japonicus)、构巢曲霉、黑曲霉、米曲霉或土曲霉。
本公开的链孢霉属真菌细胞可以包括，但不限于粗糙链孢霉。
在某些实施方式中，丝状真菌细胞不是曲霉属细胞.
在某些实施方式中，丝状真菌细胞选自木霉属(里氏木霉)、链孢霉(粗糙 链孢霉)、青霉属(产黄青霉)、曲霉属(构巢曲霉、黑曲霉和米曲霉)、毁丝霉 属(嗜热毁丝霉)和金孢子菌属(C.lucknowense)。
在某些实施方式中，丝状真菌细胞是木霉属真菌细胞。本公开的木霉属 真菌细胞可以源自野生型木霉属菌株或其突变体。合适的木霉属真菌细胞的 实例包括，但不限于哈茨木霉(Trichoderma harzianum)、康宁木霉(Trichoderma koningii)、长枝木霉(Trichoderma longibrachiatum)、里氏木霉、深绿木霉、绿 木霉、绿色木霉及其可选的有性形式(即肉座菌(Hypocrea))。
破坏丝状真菌细胞的基因和培养丝状真菌细胞的一般方法，例如，对于 青霉属，公开于Kopke等(2010)Application of the Saccharomyces cerevisiae FLP/FRTrecombination system in filamentous fungi for marker recycling andconstruction of knockout strains devoid of heterologous genes.Appl EnvironMicrobiol.76(14):4664-74.doi:10.1128/AEM.00670-10中，对于曲霉属，公开 于Maruyama和Kitamoto(2011),Targeted Gene Disruption in Koji Mold Aspergillusoryzae,James A.Williams(编辑),Strain Engineering:Methods and Protocols,Methods in Molecular Biology,vol.765,DOI 10.1007/978-l-61779-197-0_27中，对于链孢霉属，公开于Collopy等(2010) High-throughput construction of gene deletioncassettes for generation of Neurospora crassa knockout strains.Methods MolBiol.2010；638:33-40.doi: 10.1007/978-l-60761-611-5_3中和对于毁丝霉属或金孢子菌属，公开于 PCT/NL2010/000045和PCT/EP98/06496中。
丝状真菌细胞成分
本公开的特定方面涉及具有降低的或没有至少三种蛋白酶的可检测的活 性和具有编码以提高的水平(例如至少两倍高的水平)产生的异源多肽的重 组多核苷酸的丝状真菌细胞。本公开的其它方面涉及具有降低的或没有至少 三种蛋白酶的可检测的活性的木霉属真菌细胞，该至少三种蛋白酶选自pep1、 pep2、pep3、pep4、pep5、pep8、pep11、pep12、tsp1、slp1、slp2、slp7、gap1 和gap2，其中细胞进一步包含编码以比对应亲本木霉属细胞中多肽的产生水 平高至少两倍的水平产生的哺乳动物多肽的重组多核苷酸。在某些实施方式 中，丝状真菌细胞或木霉属真菌细胞具有降低的或没有至少四种、至少五种、 至少六种、至少七种、至少八种、至少九种、至少十种、至少十一种、至少 十二和或更多种蛋白酶的活性。
降低的蛋白酶的表达
本公开的丝状真菌细胞或木霉属真菌细胞中至少三种蛋白酶的降低的活 性可以是蛋白酶表达降低或消除的结果。在一些实施方式中，至少三种蛋白 酶的降低的或消除的表达是对催化结构域、编码区域或编码各蛋白酶的基因 的编码区域的表达所需的控制区域进行修饰的结果。在其它实施方式中，蛋 白酶的降低的或消除的表达是引入、替换和/或移除基因或编码各蛋白酶的基 因的转录或翻译需要的控制序列中的一个或多个核苷酸的结果。
在进一步的实施方式中，蛋白酶的降低的或消除的表达是将各包含与各 基因同源的核酸片段的破坏性核酸构建体插入编码各蛋白酶的基因中的结 果，这产生同源性区域的重复和将构建体DNA并入重复区域之间。在其它 实施方式中，蛋白酶的降低的或消除的表达是编码各蛋白酶的基因的基因转 化的结果。在再其它的实施方式中，蛋白酶的降低的或消除的表达是通过对 于编码各蛋白酶的基因中的各基因特异性的反义多核苷酸或RNAi构建体产 生的结果。在一个实施方式中，RNAi构建体对于编码天冬氨酸蛋白酶如pep-型蛋白酶、胰蛋白酶样丝氨酸蛋白酶如tsp1、谷氨酸蛋白酶如gap-型蛋白酶、 枯草溶菌素蛋白酶如slp-型蛋白酶或Sedolisin蛋白酶如tpp1或slp7蛋白酶的 基因是特异性的。在一个实施方式中，RNAi构建体对于编码slp-型蛋白酶的 基因是特异性的。在一个实施方式中，RNAi构建体对于编码slp2、slp3、slp5 或slp6的基因是特异性的。在一个实施方式中，RNAi构建体对于两种或更 多种蛋白酶是特异性的。在一个实施方式中，两种或更多种蛋白酶是pep-型 蛋白酶中的任一种、胰蛋白酶样丝氨酸蛋白酶中的任一种、slp-型蛋白酶中的任一种、gap-型蛋白酶中的任一种和/或Sedolisin蛋白酶的任一种。在一个实 施方式中，两种或更多种蛋白酶是slp2、slp3、slp5和/或slp6。在一个实施 方式中，RNAi构建体包含表22.2的核酸序列中的任一种。
在一些实施方式中，编码蛋白酶的基因各包含降低或消除相应蛋白酶活 性的突变。在其它实施方式中，突变降低或消除各蛋白酶的表达。在进一步 的实施方式中，突变是降低或消除相应蛋白酶活性的敲除突变、截断突变、 点突变、错义突变、置换突变、移码突变、插入突变、重复突变、扩增突变、 易位突变或反转突变。
在一些实施方式中，突变是蛋白酶编码基因的删除。在其它实施方式中， 突变是编码蛋白酶的催化结构域的蛋白酶编码基因部分的删除。在再其它的 实施方式中，突变是编码蛋白酶的催化结构域的蛋白酶编码基因部分中的点 突变。
蛋白酶基因的组合
本公开的丝状真菌细胞或木霉属细胞可以包含至少三种、至少四种、至 少五种、至少六种、至少七种、至少八种、至少九种、至少十种或更多种天 冬氨酸蛋白酶、胰蛋白酶样丝氨酸蛋白酶、枯草溶菌素蛋白酶和/或谷氨酸蛋 白酶。在某些实施方式中，蛋白酶由pep-型蛋白酶基因、gap-型蛋白酶基因 或slp-型蛋白酶基因编码。在一些实施方式中，pep-型蛋白酶基因选自pep1、 pep2、pep3、pep4、pep5、pep8、pep11和pep12。在其它实施方式中，gap- 型蛋白酶基因选自gap1和gap2。在进一步的实施方式中，slp-型蛋白酶基因 选自slp1、slp2、slp3和slp7或选自slp1、slp2、slp3、slp5、slp6、slp7和slp8。 在某些优选的实施方式中，slp-型蛋白酶基因是slp1。
在其它实施方式中，蛋白酶由选自pep1、pep2、pep3、pep4、pep5、 pep7、pep8、pep11、pep12、tsp1、slp1、slp2、slp3、slp5、slp6、slp7、slp8 、gap1、gap2和tpp1的基因编码。在一些实施方式中，丝状真菌细胞，例如， 木霉属细胞具有降低的或没有至少三种或至少四种选自pep1、pep2、pep3、 pep4、pep5、pep8、pep11、pep12、tsp1、slp1、slp2、slp3、slp7、gap1和gap2 的蛋白酶编码基因的表达水平。在某些实施方式中，丝状真菌细胞，例如木 霉属细胞具有降低的或没有至少三种选自pep1、tsp1和slp1的蛋白酶编码基 因的表达水平。在其它实施方式中，丝状真菌细胞或木霉属细胞具有降低的 或没有至少三种选自gap1、slp1和pep1的蛋白酶编码基因的表达水平。在一 些实施方式中，丝状真菌细胞，例如木霉属细胞具有降低的或没有蛋白酶编 码基因slp2、pep1和gap1的表达水平。在一些实施方式中，丝状真菌细胞， 例如木霉属细胞具有降低的或没有蛋白酶编码基因slp2、pep1、gap1和pep4 的表达水平。在一些实施方式中，丝状真菌细胞，例如木霉属细胞具有降低 的或没有蛋白酶编码基因slp2、pep1、gap1、pep4和slp1的表达水平。在一 些实施方式中，丝状真菌细胞，例如木霉属细胞具有降低的或没有蛋白酶编 码基因slp2、pep1、gap1、pep4、slp1和slp3的表达水平。在一些实施方式 中，丝状真菌细胞，例如木霉属细胞具有降低的或没有蛋白酶编码基因slp2、 pep1、gap1、pep4、slp1、slp3和pep3的表达水平。在一些实施方式中，丝 状真菌细胞，例如木霉属细胞具有降低的或没有蛋白酶编码基因slp2、pep1、gap1、pep4、slp1、slp3、pep3和pep2的表达水平。在一些实施方式中，丝 状真菌细胞，例如木霉属细胞具有降低的或没有蛋白酶编码基因slp2、pep1、 gap1、pep4、slp1、slp3、pep3、pep2和pep5的表达水平。在一些实施方式 中，丝状真菌细胞，例如木霉属细胞具有降低的或没有蛋白酶编码基因slp2、 pep1、gap1、pep4、slp1、slp3、pep3、pep2、pep5和tsp1的表达水平。在一 些实施方式中，丝状真菌细胞，例如木霉属细胞具有降低的或没有蛋白酶编 码基因slp2、pep1、gap1、pep4、slp1、slp3、pep3、pep2、pep5、tsp1和slp7 的表达水平。在一些实施方式中，丝状真菌细胞，例如木霉属细胞具有降低 的或没有蛋白酶编码基因slp2、pep1、gap1、pep4、slp1、slp3、pep3、pep2、 pep5、tsp1、slp7和slp8的表达水平。在一些实施方式中，丝状真菌细胞， 例如木霉属细胞具有降低的或没有蛋白酶编码基因slp2、pep1、gap1、pep4、 slp1、slp3、pep3、pep2、pep5、tsp1、slp7、slp8和gap2的表达水平。
在某些实施方式中，丝状真菌细胞的至少三种、至少四种、至少五种、 至少六种、至少七种、至少八种、至少九种、至少十种或更多种蛋白酶具有 降低的蛋白酶活性，其中具有野生型活性的相应蛋白酶各具有与SEQ ID NO: 1-16、17-36、37-57、58-65、66-81、82-97、98-117、118-128、129-144、166-181、 182-185或SEQ ID NO:491-588的氨基酸序列至少70％、至少75％、至少80％、 至少85％、至少90％、至少95％、至少96％、至少97％、至少98％、至少 99％或100％相同的氨基酸序列。在其中丝状真菌细胞是具有一种或多种蛋白酶的降低的蛋白酶活性的木霉属真菌细胞的实施方式中，其中具有野生型活 性的相应蛋白酶各具有与SEQ ID NO:1、17、37、58、66、82、98、118、129、 166或182或者SEQ ID NO:507、SEQ ID NO:522或SEQ ID NO:530的氨基 酸序列至少70％、至少75％、至少80％、至少85％、至少90％、至少95％、 至少96％、至少97％、至少98％、至少99％或100％相同的氨基酸序列。
异源多肽
本公开的丝状真菌细胞或木霉属真菌细胞包含编码异源多肽的重组多核 苷酸。在某些实施方式中，异源多肽是哺乳动物多肽。在其它实施方式中， 异源多肽是非哺乳动物多肽。
在其中丝状真菌细胞包含编码哺乳动物多肽的重组多核苷酸的实施方式 中，哺乳动物多肽可以是非糖基化的哺乳动物多肽、糖基化的哺乳动物多肽 或其组合，其包括，但不限于免疫球蛋白、抗体、生长因子和干扰素。在一 些实施方式中，哺乳动物多肽是免疫球蛋白或抗体。在其中丝状真菌细胞包 含编码免疫球蛋白或抗体的重组多核苷酸的实施方式中，丝状真菌细胞，例 如木霉属真菌细胞可以具有降低的或没有至少三种或至少四种选自pep1、 pep3、pep4、pep8、pep11、pep12、tsp1、slp1、slp2、slp7、gap1和gap2的 蛋白酶编码基因的表达。在某些优选的实施方式中，细胞，例如木霉属真菌 细胞，包含编码免疫球蛋白或抗体的重组多核苷酸并具有降低的或没有蛋白 酶编码基因slp1、slp2、slp3、tsp1、pep1、gap1、pep4、pep3、pep2、pep5 和gap2的表达。在某些优选的实施方式中，细胞，例如木霉属真菌细胞，包 含编码免疫球蛋白或抗体的重组多核苷酸并具有降低的或没有蛋白酶编码基 因pep1、tsp1、slp1和gap1的表达。在其它实施方式中，细胞包含编码免疫 球蛋白或抗体的重组多核苷酸并具有降低的蛋白酶编码基因pep1、tsp1、slp1、 gap1和pep4的表达。在其它实施方式中，细胞包含编码免疫球蛋白或抗体 的重组多核苷酸并具有降低的蛋白酶编码基因slp1、slp2和slp3的表达。在 其它实施方式中，细胞包含编码免疫球蛋白或抗体的重组多核苷酸并具有降 低的蛋白酶编码基因slp1、slp2、slp3和tsp1的表达。在其它实施方式中， 细胞包含编码免疫球蛋白或抗体的重组多核苷酸并具有降低的蛋白酶编码基 因slp1、slp2、slp3、tsp1和pep1的表达。在其它实施方式中，细胞包含编码 免疫球蛋白或抗体的重组多核苷酸并具有降低的蛋白酶编码基因slp1、slp2、 slp3、tsp1、pep1和gap1的表达。在其它实施方式中，细胞包含编码免疫球 蛋白或抗体的重组多核苷酸并具有降低的蛋白酶编码基因slp1、slp2、slp3、 tsp1、pep1、gap1和pep4的表达。在其它实施方式中，细胞包含编码免疫球 蛋白或抗体的重组多核苷酸并具有降低的蛋白酶编码基因slp1、slp2、slp3、 tsp1、pep1、gap1、pep4和pep3的表达。在其它实施方式中，细胞包含编码 免疫球蛋白或抗体的重组多核苷酸并具有降低的蛋白酶编码基因slp1、slp2、 slp3、tsp1、pep1、gap1、pep4、pep3和pep2的表达。在其它实施方式中， 细胞包含编码免疫球蛋白或抗体的重组多核苷酸并具有降低的蛋白酶编码基 因slp1、slp2、slp3、tsp1、pep1、gap1、pep4、pep3、pep2和pep5的表达。
在其它实施方式中，丝状真菌细胞包含编码生长因子、干扰素、细胞因 子或白介素的重组多核苷酸。在其中丝状真菌细胞，例如木霉属真菌细胞包 含编码生长因子、干扰素、细胞因子、人血清白蛋白或白介素的重组多核苷 酸的实施方式中，丝状真菌细胞可以具有降低的或没有至少三种或至少四种 选自pep1、pep2、pep3、pep4、pep5、pep8、gap1、gap2、slp1、slp2、slp7 和tsp1的蛋白酶编码基因的表达。在某些实施方式中，细胞包含编码生长因 子、干扰素、细胞因子、人血清白蛋白或白介素的重组多核苷酸并具有降低 的蛋白酶编码基因pep1、tsp1、slp1、gap1和gap2的表达。在某些实施方式 中，细胞包含编码生长因子、干扰素、细胞因子、人血清白蛋白或白介素的 重组多核苷酸并具有降低的蛋白酶编码基因slp1、slp2、pep1、gap1、pep4、 slp7、pep2、pep3、pep5、tsp1和gap2的表达。在其它实施方式中，细胞， 例如木霉属真菌细胞，包含编码生长因子、干扰素、细胞因子、人血清白蛋 白或白介素的重组多核苷酸并具有降低的蛋白酶编码基因pep1、tsp1、slp1、 gap1、gap2和pep4的表达。在进一步的实施方式中，细胞包含编码生长因 子的重组多核苷酸并具有降低的选自pep1、pep2、pep3、pep4和pep5的pep- 型蛋白酶基因的表达。在某些优选的实施方式中，生长因子是IGF-1或干扰 素是干扰素-α2b。在某些实施方式中，细胞包含编码生长因子、干扰素、细 胞因子、人血清白蛋白或白介素的重组多核苷酸并具有降低的蛋白酶编码基 因pep1、gap1和pep4的表达。在某些实施方式中，细胞包含编码生长因子、 干扰素、细胞因子、人血清白蛋白或白介素的重组多核苷酸并具有降低的蛋 白酶编码基因pep1、gap1、pep4和slp7的表达。在某些实施方式中，细胞包 含编码生长因子、干扰素、细胞因子、人血清白蛋白或白介素的重组多核苷 酸并具有降低的蛋白酶编码基因pep1、gap1、pep4、slp7和slp2的表达。在 某些实施方式中，细胞包含编码生长因子、干扰素、细胞因子、人血清白蛋 白或白介素的重组多核苷酸并具有降低的蛋白酶编码基因pep1、gap1、pep4、slp7、slp2和pep2的表达。在某些实施方式中，细胞包含编码生长因子、干 扰素、细胞因子、人血清白蛋白或白介素的重组多核苷酸并具有降低的蛋白 酶编码基因pep1、gap1、pep4、slp7、slp2、pep2和pep3的表达。在某些实 施方式中，细胞包含编码生长因子、干扰素、细胞因子、人血清白蛋白或白 介素的重组多核苷酸并具有降低的蛋白酶编码基因pep1、gap1、pep4、slp7、 slp2、pep2、pep3和pep5的表达。在某些实施方式中，细胞包含编码生长因 子、干扰素、细胞因子、人血清白蛋白或白介素的重组多核苷酸并具有降低 的蛋白酶编码基因pep1、gap1、pep4、slp7、slp2、pep2、pep3、pep5和slp1 的表达。在某些实施方式中，细胞包含编码生长因子、干扰素、细胞因子、 人血清白蛋白或白介素的重组多核苷酸并具有降低的蛋白酶编码基因pep1、 gap1、pep4、slp7、slp2、pep2、pep3、pep5、slp1和tsp1的表达。
在某些实施方式中，哺乳动物多肽以比没有降低的蛋白酶活性的对应亲 本丝状真菌细胞中多肽的产生水平高至少3倍、至少4倍、至少5倍、至少 6倍、至少7倍、至少8倍、至少9倍、至少10倍、至少15倍、至少20倍、 至少25倍、至少30倍、至少40倍、至少50倍、至少60倍、至少70倍、 至少75倍、至少80倍、至少90倍、至少100倍或更高倍的水平产生。在其 它实施方式中，哺乳动物多肽以比对应亲本丝状真菌细胞中多肽的全长形式 的产生水平更高的水平以全长形式产生。
在其中丝状真菌细胞包含编码非哺乳动物多肽的重组多核苷酸的实施方 式中，非哺乳动物多肽可以是氨肽酶、淀粉酶、糖酶、羧肽酶、过氧化氢酶、 纤维素酶、几丁质酶、角质酶、脱氧核糖核酸酶、酯酶、α-半乳糖苷酶、β- 半乳糖苷酶、葡糖淀粉酶、α-葡糖苷酶、β-葡糖苷酶、转化酶、漆酶、脂肪 酶、聚糖酶、氧化酶、果胶分解酶、过氧化物酶、磷脂酶、肌醇六磷酸酶、 多酚氧化酶、蛋白水解酶、核糖核酸酶、转谷氨酰胺酶或木聚糖酶。在其中 丝状真菌细胞包含编码非哺乳动物多肽的重组多核苷酸的实施方式中，丝状 真菌细胞可以具有降低的或没有至少三种、至少四种、至少五种或至少六种 选自pep1、pep2、pep3、pep4、pep5、pep8、pep11、pep12、tsp1、slp1、slp2、 slp3、gap1和gap2的蛋白酶编码基因的可检测的表达。在某些实施方式中， 非哺乳动物多肽以比对应亲本丝状真菌细胞中多肽的产生水平高至少3倍、 至少4倍、至少5倍、至少6倍、至少7倍、至少8倍、至少9倍、至少10 倍、至少15倍、至少20倍、至少25倍、至少30倍、至少40倍、至少50 倍、至少60倍、至少70倍、至少75倍、至少80倍、至少90倍、至少100 倍或更高倍的水平产生。在其它实施方式中，非哺乳动物多肽以比对应亲本 丝状真菌细胞中多肽的全长形式的产生水平更高的水平以全长形式产生。
降低的另外的蛋白酶的活性
在一些实施方式中，本公开的丝状真菌细胞或木霉属真菌细胞也具有降 低的一种或多种另外的蛋白酶的活性。在某些实施方式中，一种或多种另外 的蛋白酶的表达水平降低。在某些优选的实施方式中，编码一种或多种另外 的蛋白酶的基因各包含降低相应蛋白酶活性的突变。一种或多种另外的蛋白 酶编码基因可以是pep7、tpp1、gap2、slp3、slp5、slp6、slp7或slp8。
在某些实施方式中，当丝状真菌细胞是曲霉属细胞时，总蛋白酶活性降 低到其中蛋白酶不具有降低的活性的对应亲本曲霉属细胞中总蛋白酶活性的 50％或更低。
在某些实施方式中，总蛋白酶活性在本公开的细胞(例如木霉属细胞) 中降低到其中蛋白酶不具有降低的活性的对应亲本丝状真菌细胞中总蛋白酶 活性的49％或更低、31％或更低、13％或更低、10％或更低、6.3％或更低或 者5.5％或更低。
另外的重组修饰
在某些实施方式中，本公开的丝状真菌细胞或木霉属真菌细胞也具有降 低的长醇基-P-Man:Man(5)GlcNAc(2)-PP-长醇基甘露糖基转移酶的活性。长醇 基-P-Man:Man(5)GlcNAc(2)-PP-长醇基甘露糖基转移酶(EC 2.4.1.130)将α-D- 甘露糖残基从长醇-磷酸D-甘露糖转移到膜脂连接的寡糖中。通常，长醇基 -P-Man:Man(5)GlcNAc(2)-PP-长醇基甘露糖基转移酶通过alg3基因编码。因 此，在某些实施方式中，丝状真菌细胞具有降低的ALG3活性，其是由alg3 基因编码的活性。在一些实施方式中，alg3基因包含降低相应ALG3活性的 突变。在某些实施方式中，alg3基因从丝状真菌细胞删除。
在其它实施方式中，本公开的丝状真菌细胞或木霉属真菌细胞进一步包 含编码α-1,2-甘露糖苷酶的多核苷酸。编码α-1,2-甘露糖苷酶的多核苷酸可以 对于宿主细胞是内源的，或它可以对于宿主细胞是异源的。这些多核苷酸特 别可用于表达从高尔基体转移到ER的高甘露糖聚糖而无有效的外-α-2-甘露 糖苷酶切割的丝状真菌细胞。α-1,2-甘露糖苷酶可以是属于糖苷水解酶家族 47(cazy.org/GH47_all.html)的I型甘露糖苷酶。在某些实施方式中，α-1,2-甘 露糖苷酶是列于cazy.org/GH47_characterized.html中的酶。特别地，α-1,2-甘 露糖苷酶可以是切割糖蛋白的ER-型酶如ERα-甘露糖苷酶I EC 3.2.1.113酶亚家族。这类酶的实例包括人α-2-甘露糖苷酶1B(AAC26169)、哺乳动物ER 甘露糖苷酶的组合或丝状真菌酶如α-1,2-甘露糖苷酶(MDS1)(里氏木霉 AAF34579；Maras M等JBiotech.77,2000,255)。对于ER/高尔基体表达，甘 露糖苷酶的催化结构域通常与靶向肽如HDEL、KDEL或者ER或早期高尔基 体蛋白质的部分融合，或与动物或植物甘露糖苷酶I的内源ER靶向结构一起 表达，参见例如，Callewaert等2001Use of HDEL-taggedTrichoderma reesei mannosyl oligosaccharide 1,2-α-D-mannosidase for N-glycanengineering in Pichia pastoris.FEBS Lett 503:173-178。
在进一步的实施方式中，本公开的丝状真菌细胞或木霉属真菌细胞还包 含N-乙酰葡糖胺基转移酶I催化结构域和N-乙酰葡糖胺基转移酶II催化结构 域。这类催化结构域可用于在非哺乳动物细胞中表达复合N-聚糖。N-乙酰葡 糖胺基转移酶I(GlcNAc-TI；GnTI；EC 2.4.1.101)催化UDP-N-乙酰基-D-葡糖 胺+3-(α-D-甘露糖基)-β-D-甘露糖基-R<＝>UDP+3-(2-(N-乙酰基-β-D-葡糖 胺基)-α-D-甘露糖基)-β-D-甘露糖基-R的反应，其中R表示聚糖受体中N-连 接的寡糖的其余部分。N-乙酰葡糖胺基转移酶I催化结构域是能够催化这一 反应的N-乙酰葡糖胺基转移酶I的任何部分。N-乙酰葡糖胺基转移酶II (GlcNAc-TII；GnTII；EC 2.4.1.143)催化UDP-N-乙酰基-D-葡糖胺+6-(α-D-甘 露糖基)-β-D-甘露糖基-R<＝>UDP+6-(2-(N-乙酰基-β-D-葡糖胺基)-α-D-甘露 糖基)-β-D-甘露糖基-R的反应，其中R表示聚糖受体中N-连接的寡糖的其余 部分。N-乙酰葡糖胺基转移酶II催化结构域是能够催化这一反应的N-乙酰 葡糖胺基转移酶II的任何部分。合适的N-乙酰葡糖胺基转移酶I催化结构域 和N-乙酰葡糖胺基转移酶II催化结构域的实例可以在国际专利申请No.PCT/EP2011/070956中发现。N-乙酰葡糖胺基转移酶I催化结构域和N-乙酰 葡糖胺基转移酶II催化结构域可以通过单一多核苷酸编码。在某些实施方式 中，单一多核苷酸编码包含N-乙酰葡糖胺基转移酶I催化结构域和N-乙酰葡 糖胺基转移酶II催化结构域的融合蛋白。或者，N-乙酰葡糖胺基转移酶I催 化结构域可以通过第一多核苷酸编码和N-乙酰葡糖胺基转移酶II催化结构域 可以通过第二多核苷酸编码。
在其中丝状真菌细胞或木霉属真菌细胞包含N-乙酰葡糖胺基转移酶I催 化结构域和N-乙酰葡糖胺基转移酶II催化结构域的实施方式中，细胞还可以 包含编码甘露糖苷酶II的多核苷酸。甘露糖苷酶II能够切割GlcNAcMan5的 Man5结构以生成GlcNAcMan3，且如果与GnTII的催化结构域的作用结合， 以生成G0；且进一步地，与半乳糖基转移酶的催化结构域的作用结合，以生 成G1和G2。在某些实施方式中，II-型甘露糖苷酶属于糖苷水解酶家族38 (cazy.org/GH38_all.html)。这类酶的实例包括人酶AAC50302、黑腹果蝇(D.melanogaster)酶(Van den Elsen J.M.等(2001)EMBO J.20:3008-3017)、具有按 照PDB-记录号1HTY的3D结构的那些或以PBD中的催化结构域指代的其 它酶。对于ER/高尔基体表达，甘露糖苷酶的催化结构域通常与N-末端靶向 肽融合，例如使用国际专利申请No.PCT/EP2011/070956中所列的或SEQ ID NO:589-594的靶向肽。在用II-型甘露糖苷酶的甘露糖苷酶催化结构域转化 后，选择有效产生GlcNAc2Man3、GlcNAclMan3或G0的菌株。
在可以与前述实施方式结合的某些实施方式中，丝状真菌细胞进一步包 含编码UDP-GlcNAc转运蛋白的多核苷酸。
在可以与前述实施方式结合的某些实施方式中，丝状真菌细胞进一步包 含编码β-1,4-半乳糖基转移酶的多核苷酸。一般地，β-1,4-半乳糖基转移酶属 于CAZy半乳糖基转移酶家族7(cazy.org/GT7_all.html)。可用的β4GalT酶的 实例包括β4GalT1，例如牛(黄牛)酶AAA30534.1(Shaper N.L.等Proc.Natl. Acad.Sci.U.S.A.83(6),1573-1577(1986))、人酶(Guo S.等Glycobiology 2001, 11:813-20)和小鼠酶AAA37297(Shaper,N.L.等1998J.Biol.Chem.263(21), 10420-10428)。在其中丝状真菌细胞包含编码半乳糖基转移酶的多核苷酸的 本发明的某些实施方式中，丝状真菌细胞还包含编码UDP-Gal 4表异构酶和/ 或UDP-Gal转运蛋白的多核苷酸。在其中丝状真菌细胞包含编码半乳糖基转 移酶的多核苷酸的本发明的某些实施方式中，乳糖可以代替葡萄糖用作培养 宿主细胞时的碳源。培养基可以在pH 4.5和7.0之间或5.0和6.5之间。在其 中丝状真菌细胞包含编码半乳糖基转移酶的多核苷酸和任选的编码UDP-Gal 4表异构酶和/或UDP-Gal转运蛋白的多核苷酸的本发明的某些实施方式中， 二价阳离子如Mn2+、Ca2+或Mg2+可以添加到细胞培养基中。
在可以与前述实施方式结合的某些实施方式中，宿主细胞中α-1,6-甘露 糖基转移酶的活性水平与野生型宿主细胞中的活性水平相比降低。在某些实 施方式中，丝状真菌具有与野生型丝状真菌细胞中的表水平相比降低的och1 基因的表达水平。
另一个方面包括在丝状真菌细胞中产生Man3GlcNAc2N-聚糖[即 Manα3(Manα6)Manβ4GlcNAcβ4GlcNAc]的方法，包括提供具有编码异源多肽 的重组多核苷酸和具有与野生型丝状真菌细胞中的活性水平相比降低的alg3 甘露糖基转移酶活性水平的丝状真菌细胞，和培养该丝状真菌细胞以产生 Man3GlcNAc2聚糖的步骤，其中Man3GlcNAc2聚糖占丝状真菌细胞分泌的 中性N-聚糖的至少10％、至少20％、至少30％、至少40％、至少50％、至 少60％、至少70％、至少80％、至少90％或100％(mol％)。在特定实施方式 中，Man3GlcNAc2N-聚糖代表异源多肽的总N-聚糖的至少10％、至少20％、 至少30％、至少40％、至少50％、至少60％、至少70％、至少80％、至少 90％或100％(mol％)。
另一个方面包括在丝状真菌细胞中产生复合N-聚糖(即，包含末端 GlcNAc2Man3结构的N-聚糖)，例如GlcNAc2Man3GlcNAc2{即G0，即 GlcNAcβ2Manα3(GlcNAcβ2Manα6)Manβ4GlcNAcβ4GlcNAc}聚糖的方法，包 括提供具有编码异源多肽的重组多核苷酸、与野生型丝状真菌细胞中的活性 水平相比具有降低的alg3甘露糖基转移酶活性水平且进一步包含编码N-乙 酰葡糖胺基转移酶I催化结构域的多核苷酸和编码N-乙酰葡糖胺基转移酶II 催化结构域的多核苷酸的丝状真菌细胞，和培养该丝状真菌细胞以产生复合 N-聚糖，例如GlcNAc2Man3GlcNAc2聚糖的步骤，其中GlcNAc2Man3GlcNAc2聚糖占丝状真菌细胞分泌的中性N-聚糖的至少5％、 至少10％、至少20％、至少30％、至少40％、至少50％、至少60％、至少70％、至少80％、至少90％或100％(mol％)。在某些实施方式中，复合N- 聚糖，例如GlcNAc2Man3GlcNAc2聚糖，代表多肽的总N-聚糖的至少5％、 至少10％、至少20％、至少30％、至少40％、至少50％、至少60％、至少 70％、至少80％、至少90％或100％(mol％)。在某些实施方式中，所述复合 N-聚糖是GlcNAcMan3和/或GlcNAc2Man3。
另一个方面包括在丝状真菌细胞中产生G1或G2N-聚糖或其混合物，例 如GalGlcNAc2Man3GlcNAc2{即G1，即 Galβ4GlcNAcβ2Manα3(GlcNAcβ2Manα6)Manβ4GlcNAcβ4GlcNAc}或 GlcNAcβ2Manα3(Galβ4GlcNAcβ2Manα6)Manβ4GlcNAcβ4GlcNAc}和/或Gal2GlcNAc2Man3GlcNAc2{即G2，即 Galβ4GlcNAcβ2Manα3(Galβ4GlcNAcβ2Manα6)Manβ4GlcNAcβ4GlcNAc}聚糖 的方法，包括提供具有编码异源多肽的重组多核苷酸和与野生型丝状真菌细 胞中的活性水平相比具有降低的alg3甘露糖基转移酶活性水平且进一步包含编码N-乙酰葡糖胺基转移酶I催化结构域的多核苷酸、编码N-乙酰葡糖胺基 转移酶II催化结构域的多核苷酸和编码GalT催化结构域的多核苷酸的丝状 真菌细胞，和培养该丝状真菌细胞以产生G1或G2N-聚糖或其混合物的步骤， 其中G1聚糖占丝状真菌细胞分泌的中性N-聚糖的至少5％、至少10％、至 少20％、至少30％、至少40％、至少50％、至少60％、至少70％、至少80％、 至少90％或100％(mol％)，或其中G2聚糖占丝状真菌细胞分泌的中性N-聚 糖的至少5％、至少10％、至少20％、至少30％、至少40％、至少50％、至 少60％、至少70％、至少80％、至少90％或100％(mol％)。在特定实施方式 中，G1聚糖占多肽的总N-聚糖的至少10％、至少20％、至少30％、至少40％、 至少50％、至少60％、至少70％、至少80％、至少90％或100％(mol％)。在 特定实施方式中，G2聚糖占多肽的总N-聚糖的至少10％、至少20％、至少 30％、至少40％、至少50％、至少60％、至少70％、至少80％、至少90％或 100％(mol％)。
在某些实施方式中，产生复合N-聚糖的方法生成不同聚糖的混合物。复 合N-聚糖或Man3GlcNAc2可以占这种聚糖混合物的至少5％、至少10％、至 少20％、至少30％、至少40％、至少50％、至少60％、至少70％、至少80％ 或至少90％或者更高。在某些实施方式中，多肽的N-聚糖的至少5％、至少 10％、至少20％、至少30％、至少40％、至少50％、至少60％、至少70％、 至少80％或至少90％或者更多由这种聚糖混合物组成。在某些实施方式中， 产生复合N-聚糖及G1和/或G2N-聚糖的方法生成不同聚糖的混合物。复合 N-聚糖、Man3GlcNAc2、G1和/或G2可以占这种聚糖混合物的至少5％、至 少10％、至少20％、至少30％、至少40％、至少50％、至少60％、至少70％、 至少80％或至少90％或者更高。在某些实施方式中，多肽的N-聚糖的至少 5％、至少10％、至少20％、至少30％、至少40％、至少50％、至少60％、 至少70％、至少80％或至少90％或者更多由这种聚糖混合物组成。
在某些实施方式中，需要的是产生杂合N-聚糖的方法。如本文中使用的， 术语“杂合”意思是具有N-乙酰葡糖胺连接的包含未取代的末端甘露糖残基 (如存在于高甘露糖聚糖中)和取代的甘露糖残基两者的聚糖，例如 GlcNAcβ2Manα3[Manα3(Manα6)Manα6]Manβ4GlcNAcβ4GlcNAc。在这样的实 施方式中，Man5{即Manα3[Manα3(Manα6)Manα6]Manβ4GlcNAcβ4GlcNAc} 表达丝状真菌细胞如里氏木霉菌株用编码异源多肽的重组多核苷酸和编码 N-乙酰葡糖胺基转移酶I催化结构域的多核苷酸转化且培养该丝状真菌细胞 以产生杂合N-聚糖，其中杂合N-聚糖占丝状真菌细胞分泌的中性N-聚糖的 至少5％、至少10％、至少20％、至少30％、至少40％、至少50％、至少60％、 至少70％、至少80％、至少90％或100％(mol％)。在特定实施方式中，多肽 的N-聚糖的至少10％、至少20％、至少30％、至少40％、至少50％、至少 60％、至少70％、至少80％、至少90％或100％(mol％)由杂合N-聚糖组成。
Man3GlcNAc2，复合的、杂合的、G1和G2N-聚糖可以连接于选自氨基 酸、肽和多肽的分子。在某些实施方式中，Man3GlcNAc2，复合的、杂合的、 G1和G2N-聚糖连接于异源多肽。在某些实施方式中，异源多肽是糖基化蛋 白质。在特定实施方式中，糖基化多肽是哺乳动物多肽。在某些实施方式中， 哺乳动物多肽是抗体或其抗原结合片段。
在某些实施方式中，糖基转移酶，例如GnTI、GnTII或GalT或者糖基 水解酶，例如，α-1,2-甘露糖苷酶或甘露糖苷酶II，包括与催化结构域连接的 靶向肽。如本文中使用的，术语“连接”意思是两个氨基酸残基聚合物(在多 肽的情况中)或两个核苷酸聚合物(在多核苷酸的情况中)彼此相邻地直接 偶联或处于相同多肽或多核苷酸内但由插入氨基酸残基或核苷酸分隔。如本 文中使用的，“靶向肽”是指能够将重组蛋白质定位于丝状真菌细胞内的内质 网(ER)或高尔基体(Golgi)的重组蛋白中的任何数目的连续氨基酸残基。靶向肽可以是催化结构域的N-末端或C-末端。在某些实施方式中，靶向肽是催化 结构域的N-末端。在某些实施方式中，靶向肽提供与ER或高尔基体膜的直 接结合。靶向肽的成分可以来自于正常存在于ER或高尔基体中的任何酶。 这样的酶包括甘露糖苷酶、甘露糖基转移酶、糖基转移酶、2型高尔基体蛋 白质及MNN2、MNN4、MNN6、MNN9、MNN10、MNS1、KRE2、VAN1 和OCH1酶。合适的靶向肽描述于国际专利申请No.PCT/EP2011/070956中。 在一个实施方式中，GnTI或GnTII的靶向肽是人GnTII酶。在其它实施方式 中，靶向肽源自木霉属Kre2、Kre2-样、Och1、Anp1和Van1。在一个实施方 式中，靶向肽选自SEQ ID NO:589-594的组。
本发明丝状真菌细胞的用途
本文的本发明进一步涉及使用本公开的任何丝状真菌细胞如木霉属真菌 细胞的方法，该细胞具有降低的或没有至少三种蛋白酶的蛋白酶活性和包含 编码以提高的水平产生的异源多肽如哺乳动物多肽的重组多核苷酸，用于提 高异源多肽稳定性和产生异源多肽。测量蛋白质稳定性和产生异源多肽的方 法是公知的，并包括，但不限于本公开中描述的所有方法和技术。
因此，本公开的特定实施方式涉及通过以下方式提高异源多肽稳定性的 方法：a)提供具有降低的或没有至少三种蛋白酶的活性的本公开丝状真菌细 胞，其中该细胞进一步包含编码异源多肽的重组多核苷酸；和b)培养该细胞 以使得表达异源多肽，其中与不包含编码蛋白酶的基因的突变的宿主细胞相 比，该异源多肽具有提高的稳定性。本公开的其它实施方式涉及通过以下方 式提高哺乳动物多肽稳定性的方法：a)提供具有降低的或没有至少三种蛋白 酶的活性的本公开木霉属真菌细胞，其中该细胞进一步包含编码哺乳动物多 肽的重组多核苷酸；和b)培养该细胞以使得表达哺乳动物多肽，其中与不包 含编码蛋白酶的基因的突变的宿主细胞相比，哺乳动物多肽具有提高的稳定 性。丝状真菌细胞或木霉属真菌细胞可以是题为“本发明的丝状真菌细胞”的 章节中描述的任何细胞。测量多肽稳定性和培养丝状真菌细胞和/或木霉属真 菌细胞的方法是本领域中公知的，且包括，但不限于本公开中描述的所有方 法和技术。
在某些实施方式中，异源多肽或哺乳动物多肽的稳定性与对应亲本丝状 真菌细胞或木霉属真菌细胞中表达的异源多肽或哺乳动物多肽相比提高至少 2倍、至少3倍、至少4倍、至少5倍、至少6倍、至少7倍、至少8倍、 至少9倍、至少10倍、至少15倍、至少20倍、至少25倍、至少30倍、至 少40倍、至少50倍、至少60倍、至少70倍、至少75倍、至少80倍、至 少90倍、至少100倍或更高倍。
本公开的其它实施方式涉及通过以下方式制备异源多肽的方法：a)提供 具有降低的或没有至少三种蛋白酶的活性的本公开丝状真菌细胞，其中该细 胞进一步包含编码异源多肽的重组多核苷酸；b)培养该细胞以使得表达异源 多肽；和c)纯化所述异源多肽。本公开的进一步的实施方式涉及通过以下方 式制备哺乳动物多肽的方法：a)提供具有降低的或没有至少三种蛋白酶的活 性的本公开木霉属真菌细胞，其中该细胞进一步包含编码哺乳动物多肽的重 组多核苷酸；b)培养该宿主细胞以使得表达哺乳动物多肽；和c)纯化所述 哺乳动物多肽。丝状真菌细胞或木霉属真菌细胞可以是题为“本发明的丝状真 菌细胞”的章节中描述的任何细胞。培养丝状真菌或木霉属真菌细胞和纯化多 肽的方法是本领域中公知的，且包括，但不限于本公开中描述的所有方法和 技术。
在某些实施方式中，丝状真菌细胞或木霉属真菌细胞在选自pH 3.5-7、 pH 3.5-6.5、pH 4-6、pH 4.3-5.7、pH 4.4-5.6和pH 4.5-5.5的pH范围中培养。 在某些实施方式中，为产生抗体，丝状真菌细胞或木霉属真菌细胞在选自 4.7-6.5、pH 4.8-6.0、pH 4.9-5.9和pH 5.0-5.8的pH范围中培养。
在一些实施方式中，异源多肽是哺乳动物多肽。在其它实施方式中，异 源多肽是非哺乳动物多肽。
在某些实施方式中，哺乳动物多肽选自免疫球蛋白、免疫球蛋白重链、 免疫球蛋白轻链、单克隆抗体、杂合抗体、F(ab')2抗体片段、F(ab)抗体片段、 Fv分子、单链Fv抗体、二聚的抗体片段、三聚的抗体片段、功能性抗体片 段、单域抗体、多聚单域抗体、免疫粘附分子、胰岛素样生长因子1、生长 激素、胰岛素和促红细胞生成素。在其它实施方式中，哺乳动物蛋白是免疫 球蛋白或胰岛素样生长因子1。在再其它的实施方式中，哺乳动物蛋白是抗 体。在进一步的实施方式中，哺乳动物多肽的产率是至少0.5、至少1、至少 2、至少3、至少4或至少5克/升。在某些实施方式中，哺乳动物多肽是抗体， 任选地IgG1、IgG2、IgG3或IgG4。在进一步的实施方式中，抗体的产率是 至少0.5、至少1、至少2、至少3、至少4或至少5克/升。在再其它的实施 方式中，哺乳动物多肽是生长因子或细胞因子。在进一步的实施方式中，生 长因子或细胞因子的产率是至少0.1、至少0.2、至少0.3、至少0.4、至少0.5、 至少1、至少1.5、至少2、至少3、至少4或至少5克/升。在进一步的实施 方式中，哺乳动物多肽是抗体，且该抗体包含至少70％、至少80％、至少 90％、至少95％或至少98％的天然抗体C-末端和N-末端而没有另外的氨基 酸残基。在其它实施方式中，哺乳动物多肽是抗体，且该抗体包含至少70％、 至少80％、至少90％、至少95％或至少98％的天然抗体C-末端和N-末端，其不缺少任何C-末端或N-末端氨基酸残基。
在其中哺乳动物多肽从细胞培养物纯化的某些实施方式中，含有哺乳动 物多肽的培养物包含占到所产生多肽的质量的少于50％、少于40％、少于 30％、少于20％或少于10％的质量百分比的多肽片段。在某些优选的实施方 式中，哺乳动物多肽是抗体，且多肽片段是重链片段和/或轻链片段。在其中 哺乳动物多肽是抗体且多肽从细胞培养物纯化的其它实施方式中，含有抗体 的培养物包含占到所产生抗体的质量的少于50％、少于40％、少于30％、少 于20％或少于10％的质量百分比的游离重链和/或游离轻链。测定多肽片段的 质量百分比的方法是本领域中公知的且包括测量SDS-凝胶的信号强度。
在进一步的实施方式中，非哺乳动物多肽选自氨肽酶、淀粉酶、糖酶、 羧肽酶、过氧化氢酶、纤维素酶、壳多糖酶、角质酶、脱氧核糖核酸酶、酯 酶、α-半乳糖苷酶、β-半乳糖苷酶、葡糖淀粉酶、α-葡糖苷酶、β-葡糖苷酶、 转化酶、漆酶、脂肪酶、聚糖酶、氧化酶、果胶分解酶、过氧化物酶、磷脂 酶、肌醇六磷酸酶、多酚氧化酶、蛋白水解酶、核糖核酸酶、转谷氨酰胺酶 或木聚糖酶。
在任何公开的方法的特定实施方式中，该方法包括提供一种或多种、两 种或更多种、三种或更多种、四种或更多种或者五种或更多种蛋白酶抑制剂 的另外的步骤。在某些实施方式中，蛋白酶抑制剂是与哺乳动物多肽共表达 的肽。在其它实施方式中，抑制剂抑制选自天冬氨酸蛋白酶、胰蛋白酶样丝 氨酸蛋白酶、枯草溶菌素蛋白酶和谷氨酸蛋白酶的蛋白酶家族的至少两种、 至少三种或至少四种蛋白酶。
在任何公开的方法的特定实施方式中，丝状真菌细胞或木霉属真菌细胞 还包含载体蛋白。如本文中使用的，“载体蛋白”是丝状真菌细胞或木霉属真 菌细胞内源的或由丝状真菌细胞或木霉属真菌细胞高度分泌的蛋白质的部 分。合适的载体蛋白包括，但不限于里氏木霉甘露聚糖酶I(Man5A或MANI)、 里氏木霉纤维二糖水解酶II(Cel6A或CBHII)(参见，例如，Paloheimo等Appl. Environ.Microbiol.2003December；69(12):7073-7082)或里氏木霉纤维二糖水 解酶I(CBHI)的那些。在一些实施方式中，载体蛋白是CBH1。在其它实施方式中，载体蛋白是截短的里氏木霉CHB1蛋白，其包括CHB1核心区和 CHB1连接体区域的部分。在一些实施方式中，载体如纤维二糖水解酶或其 片段与抗体轻链和/或抗体重链融合。在一些实施方式中，载体如纤维二糖水 解酶或其片段与胰岛素样生长因子1、生长激素、胰岛素、干扰素α2b、成 纤维细胞生长因子21或人血清白蛋白融合。在一些实施方式中，载体-抗体 融合多肽包含Kex2切割位点。在某些实施方式中，Kex2或其它载体切割酶 是丝状真菌细胞内源的。在某些实施方式中，载体切割蛋白酶是对于丝状真 菌细胞异源的，例如，源自酵母的另一种Kex2蛋白或TEV蛋白酶。在某些 实施方式中，载体切割酶过表达。
应理解的是，尽管本发明已结合其某些具体的实施方式进行了描述，前 述说明意图阐明而非限制本发明的范围。本发明范围内的其它方面、优势和 改进对于本发明所属领域的技术人员是明显的。
尽管已经描述了本发明，以下实施例提供用于通过说明的方式而不是限 制的方式阐明本发明。
实施例
实施例1—里氏木霉中天冬氨酸蛋白酶的鉴定
这一实施例证明了来自里氏木霉(T.reesei)培养上清液的天冬氨酸蛋白酶 降解抗体重链和轻链的能力。
天冬氨酸蛋白酶的纯化
据发现里氏木霉上清液中的蛋白酶活性可以用天冬氨酸蛋白酶抑制剂胃 蛋白酶抑制剂A抑制。因此，胃蛋白酶抑制剂A(Sigma#P2032)经由二氨基 二丙基胺连接体与琼脂糖珠连接，并用作用于纯化的亲和树脂。里氏木霉分 批补料发酵上清液(15ml)用于将蛋白酶分批结合35ml含50mM柠檬酸钠、 0.2M NaCl的缓冲液,pH 3.0中的树脂。柱用相同的结合缓冲液洗涤且结合的 蛋白质用洗脱缓冲液(50mM Tris-HCL,1M NaCl,pH 8.5)去除。收集0.5ml 的级分。纯化总共42μg的蛋白酶。峰级分包含0.04μg/μl蛋白质。30μl的 各级分与6μl含β-巯基乙醇的Laemmli样品缓冲液混合。样品在95℃下加 热5分钟，然后与宽范围的预染色分子量标志物(BioRad)一起加载到4-15％ PAGE凝胶(BioRad mini-protean TGX预制凝胶)中。凝胶在SDS PAGE电泳缓 冲液中100V下运行30分钟，且然后用GelCode(ThermoScientific)蓝染染色。
42kD双峰带在胃蛋白酶抑制剂A亲和柱中纯化(图1)，并从SDS PAGE 凝胶切除和用测序级修饰胰蛋白酶(Promega#V5111)进行凝胶中胰蛋白酶消 化。所得的肽然后从凝胶提取并通过C18ZipTip(Millipore#ZTC18M096)纯 化。纯化的肽在QSTAR Pulsar,ESI-混合四极-TOF(AB Sciex)上通过 LC-MS/MS分析。
这一分析导致鉴别具有非常类似的分子量的4种天冬氨酸蛋白酶。鉴别 的蛋白酶包括：pep1(Tre74156；42.7kD,42％序列覆盖率)、pep2(Tre53961； 42.4kD,15％序列覆盖率)、pep3(Tre121133；49kD,6％序列覆盖率)和pep5 (Tre81004；45kD,9％序列覆盖率)。这些天冬氨酸蛋白酶在PAGE凝胶中以相 似的分子量运行。它们的氨基酸序列相似性在51％-64％之间。
来自峰级分(F3)的蛋白质(0.8μg)然后在37℃下与柠檬酸钠缓冲液(50 mM,pH5.5)中的IgG(50μg/ml)孵育20小时(图2)。蛋白质在10uM胃蛋白酶 抑制剂A存在或不存在的情况下孵育。抗体混合物与Laemmli样品缓冲液组 合并在95℃下加热5分钟。这些样品然后与宽范围预染色分子量标志物 (BioRad)一起加载到4-15％PAGE凝胶(BioRad mini-protean TGX预制凝胶) 中。凝胶在100V下于SDS PAGE电泳缓冲液中运行30分钟。在凝胶上运行 之前，IgG不还原。全尺寸IgG恰在200kDa标志物之上。如可在图2的非 还原凝胶中看到的，天冬氨酸蛋白酶能够产生IgG的轻度降解。此外，IgG 降解被胃蛋白酶抑制剂A抑制。天冬氨酸蛋白酶活性在pH 5.5下比在酸性 pH下受到更大限制，它们在酸性pH下具有最大活性。
pep1删除的分析
天冬氨酸蛋白酶pep1蛋白酶然后进行测试以确定其在里氏木霉中的丰 度。这通过从源自pep1删除菌株M182的上清液样品纯化天冬氨酸蛋白酶来 进行。M182 pep1删除菌株也产生利妥昔单抗抗体。
在基础菌株M124中形成的M181 pep1删除菌株在大的摇瓶培养中与 M124对照瓶一起生长。培养物在具有4g/L乳糖、2g/L废谷物提取物(spent grain extract)和100mMPIPPS的300ml的TrMM,pH 5.5中生长。三种不同 的模型抗体在pep1删除菌株和其亲本菌株M124的摇瓶培养上清液(在柠檬 酸钠缓冲液pH 5.5中稀释2mg/ml)中孵育(0.05μg/μl终浓度)，且作为对比 在亲本菌株的发酵培养上清液中孵育。来自第5天培养物的含抗体的上清液 样品(30μl)加载到4-15％SDS PAGE凝胶中并转移到硝基纤维素上以用于用 在TBST中1:30,000稀释的抗-重链AP偶联抗体(Sigma#A3188)或抗-轻链AP 偶联物(Sigma#A3813)进行免疫印迹分析。当与抗体孵育18小时过夜时， Δpep1上清液较少的重链蛋白质，如与M124对照菌株或发酵上清液(pH 5.5； 28℃；20g/L废谷物提取物，60g/L乳糖)对比的(图39)。与轻链相比，重链更 易降解。最大的稳定性效应对于利妥昔单抗和MAB01重链是明显的。在重 链中，两种不同的降解产物可以在～48kD和～38kD处看到(图39)。与对照相 比，在Δpep1上清液中轻链蛋白的稳定性中仅具有轻微改善(图39)。
pep1删除质粒的产生
设计了用于pep1(TreID74156)的第一删除构建体以使得能够在成功整合 后从里氏木霉基因组去除选择标记并从而再循环选择标记用于后续的蛋白酶 基因删除。在这种方法中，标记的再循环(即从删除构建体去除pyr4基因) 类似于针对酵母开发的所谓blaster盒(Hartl,L.和Seiboth,B.,2005,Curr Genet 48:204-211及Alani,E.等,1987,Genetics 116:541-545)。类似的blaster盒也已 针对丝状真菌包括红褐肉座菌(Hypocreajecorina)(无性型：里氏木霉)开发 (Hartl,L.和Seiboth,B.,2005,Curr Genet 48:204-211)。
TreID编号是指来自Joint Genome Institute里氏木霉v2.0基因组数据库的 特定蛋白酶基因的识别编号。用于构建删除质粒的引物“经眼(by eye)”或使用 Primer3软件设计(Primer3网站，Rozen和Skaletsky(2000)Bioinformatics Methods and Protocols:Methods in Molecular Biology.Humana Press,Totowa, NJ,pp 365-386)。
使用pyr4作为标记基因的blaster盒的原理如下：pyr4，编码里氏木霉的 乳清苷-5'-单磷酸(OMP)脱羧酶(Smith,J.L.等,1991,Current Genetics 19:27-33)，是尿苷合成所需要的。缺乏OMP脱羧酶活性的菌株不能在没有尿 苷补充的最低培养基上生长(即，是尿苷营养缺陷型)。5-氟乳清酸(5-FOA) 用于产生缺乏OMP脱羧酶活性的突变菌株(pyr4-菌株)是基于5-FOA通过 OMP脱羧酶转化为毒性中间体5-氟-UMP。因此，具有突变的pyr4基因的细 胞是5-FOA抗性的，但此外也是尿苷营养缺陷的。5-FOA抗性原则上也可能 由另一基因(pyr2，乳清酸磷酸核糖转移酶)中的突变产生，且因此利用这一选 择获得的自发突变体需要通过补充具有pyr4基因的突变体对pyr4-基因型进 行验证。一旦突变，pyr4基因可以用作里氏木霉中的营养缺陷型选择标记。 在我们的blaster盒中，pyr4接着pyr4 5'未翻译区(5'UTR)的308bp同向重复 序列并由待删除基因的5'和3'侧翼区域包围。删除盒的整合通过功能pyr4选 择。pyr4标记的去除随后在5-FOA的存在下通过两个同源区域(5'UTR的同向重复序列)之间的重组强制进行，导致选择标记的环出(looping out)并使得 能够在后续轮的基因删除中利用相同的blaster盒(pyr4环出)。在环出后，仅 5'UTR的308bp序列保留在基因座中。
因此，pyr4选择标记和5'同向重复片段(pyr4 5'UTR的308bp)使用质粒 pARO502(包含里氏木霉pyr4的基因组拷贝)作为模板通过PCR产生。PCR 扩增用Phusion聚合酶和HF缓冲液或GC缓冲液进行，或用Dynazyme EXT 聚合酶进行。反应条件基于所扩增的片段变化。两个片段包含需要在酵母中 利用同源重组克隆具有环出盒的质粒的40bp重叠序列(参见下文)。为使得 能够实现可能的另外的克隆步骤，AscI消化位点置于pyr4标记和5'同向重复 序列之间且NotI位点包围完整blaster盒。
1066bp的5'侧翼区域和1037bp的3'侧翼区域选择作为pep1删除质粒的 基础。片段通过PCR产生。产物用琼脂糖凝胶电泳分离且正确的片段使用标 准实验室方法利用凝胶提取试剂盒(Qiagen)从凝胶分离。用于侧翼区域扩增的 模板DNA来自里氏木霉野生型菌株QM6a(ATCC13631)。
对于克隆中使用的酵母同源重组系统，对适宜的PCR-引物设置用于载体 和选择标记的重叠序列。为使标记能够在构建体中切换，NotI限制性位点在 侧翼区域和选择标记之间引入。PmeI限制性位点设置在载体和侧翼区域之间 用于在转化到里氏木霉中之前去除载体序列。载体骨架pRS426用限制性酶 (EcoRI和XhoI)消化。限制性片段然后用琼脂糖凝胶电泳分离，且正确的片 段使用标准实验室方法利用凝胶提取试剂盒(Qiagen)从凝胶分离。
为构建删除质粒，载体骨架及适宜的标记和侧翼区域片段转化到酿酒酵 母(菌株H3488/FY834)中。酵母转化方案是基于描述于Colot和Collopy的链 孢霉敲除工作组材料(Dartmouth链孢霉基因组方案网站)和Gietz实验室方案 (University of Manitoba,Gietz实验室网站)中的同源酵母重组的方法。来自酵 母转化体的质粒DNA通过转化到大肠杆菌中拯救。几个克隆被培养，质粒 DNA被分离和消化以使用标准实验室方法筛选正确的重组。具有正确插入大 小的几个克隆进行测序并储存。
用于pep1的第一删除质粒(质粒pTTv41，表1.1)使用另一选择标记bar， 携带链霉菌的草铵膦N-乙酰转移酶(GenBank ID:AF013602.1,Sweigard等, 1997,Fungal GenetNewsl 44:52-53)的合成构建体。侧翼区域和标记片段通过 PCR产生并使用如上所述的酵母重组方法组装到质粒中。为克隆第二pep1 删除质粒(pTTv71,表1.1)，bar标记利用NotI消化从删除质粒pTTv41除去 并使用酵母同源重组系统以上述pyr4 blaster盒替代。这些用于pep1的删除 质粒(pTTv41和pTTv71)导致pep1基因座中的1874 bp删除并覆盖PEP1的完 整编码序列。
表1.1:用于产生pep1删除质粒的引物
用于pep1的删除质粒pTTv41(TreID74156)，载体骨架pRS426
用于pep1(TreID74156)的删除质粒pTTv71，载体骨架pTTv41
pep1删除菌株M181和M195的产生
为能够再循环选择标记和允许快速删除后续蛋白酶基因，pep1使用如上 所述的pyr4 blaster盒从M127(基础菌株M124的pyr4-突变体)删除。为除去 载体序列，质粒pTTv71(Δpep1-pyr4)用PmeI消化且正确的片段使用QIAquick 凝胶提取试剂盒(Qiagen)从琼脂糖凝胶纯化。大约5μg的pep1删除盒用于转 化菌株M127。原生质体的制备和用于pyr4选择的转化基本上按照等(1987,Gene 61:155-164)和Gruber等(1990,Curr.Genet.18:71-76)的方法 进行。
200个克隆挑取作为选择性划线。作为选择性划线快速生长的24个转化 体使用标准实验室方法利用表1.2中所列的引物通过PCR筛选正确整合。七 个推定的破坏体纯化为单细胞克隆。pep1的删除使用标准实验室方法通过这 些克隆的DNA印迹分析验证(图3A)。用于DNA印迹分析的DNA利用用于 基因组DNA分离的Easy-DNA试剂盒(Invitrogen)分离。DNA印迹分析基本 上按照Sambrook等(1989,Molecular Cloning:A laboratorymanual.2nd Ed., Cold Spring Harbor Laboratory Press)中用于同源杂交的方案使用放射性标记 (32P)，HexaLabel Plus或DecaLabel Plus试剂盒(Fermentas)进行。Southern消化方案使用用于Windows 95的Sci Ed Central(Clone Manager 5for Windows 95)或Geneious Pro 5.3.6软件(Geneious网站)设计。DNA印迹分析也证实四个 克隆是单一整合体(图3B和3C)。三个克隆表明删除盒的多重和不精确的整 合并放弃。两个纯克隆以菌株编号M181(9-20A-1)和M195(9-35A-1)命名。
产生利妥昔单抗的pep1删除菌株M182的产生
为去除载体序列，质粒pTTv41(Δpep1-bar)用PmeI消化且正确的片段使 用QIAquick凝胶提取试剂盒(Qiagen)从琼脂糖凝胶纯化。大约5μg的pep1 删除盒用于转化菌株M169(表达调和的(harmonized)利妥昔单抗抗体)。原生 质体的制备和转化按照Penttila等(1987)和Avalos等(1989)中描述的方法进 行。
大约100个克隆挑取作为选择性划线。作为选择性划线快速生长的24 个转化体使用标准实验室方法通过PCR(使用表1.2中所列的引物)筛选正 确整合。八个推定的破坏体纯化为单细胞克隆。pep1的删除如以上对于M181 和M195描述的使用标准实验室方法通过五个克隆的DNA印迹分析验证(图4A)。DNA印迹分析也证实四个克隆是单一整合体(图4B和4C)。一个克隆 表明删除盒的多重和不精确的整合并放弃。一个纯克隆(11-1A)以菌株编号M182命名。
表1.2:用于筛选pep1删除构建体的整合的引物
产生利妥昔单抗的pep1删除菌株M182的分析
M182菌株在28℃下和补充有20g/l废谷物提取物、60g/l乳糖和8.1g/l 酪蛋白氨基酸的木霉属最低培养基(TrMM)，pH 5.5中生长。七微克天冬氨酸 蛋白酶从15ml上清液回收。当纯化的级分在4-15％SDS PAGE凝胶(BioRad mini-protean TGX预制凝胶)上运行时，先前在亲本菌株中观察到的42kD分 子量条带消失(图5)。仅可以看见约40kD的微弱条带。40kD条带可能对应 于次要天冬氨酸蛋白酶。第二纯化由其中存在pep1的培养上清液进行。M169 菌株产生利妥昔单抗且不包含pep1蛋白酶删除。菌株在28℃下补充20g/l 废谷物提取物、60g/l乳糖和8.1g/l酪蛋白氨基酸的木霉属最低培养基，pH 5.5 中生长。17μg天冬氨酸蛋白酶从15ml上清液纯化，并在SDS PAGE凝胶上 显示42kD条带(图5)。按照这一分析，每15ml培养上清液产生大约10μg 的pep1蛋白酶。即上清液中约60％的总天冬氨酸蛋白酶和仅约0.04％的总蛋 白含量。这一数据证明pep1是里氏木霉中最丰富的天冬氨酸蛋白酶。
其它天冬氨酸蛋白酶的分析
pep2的删除只显示抗体重链产生和降低总蛋白酶活性的轻微提高(图6 和7)。
因此，pep3和pep5是待删除的下一重要蛋白酶，特别是在pep1/tsp1/slp1 三重删除菌株中，因为它们仍构成三重删除菌株上清液中剩余蛋白酶活性的 最多一半。
pep2删除质粒的产生
用于天冬氨酸蛋白酶pep2(TreID0053961)的pTTv96删除质粒基本上如 以上对于pTTv41 pep1删除质粒描述的构建。920bp的5'侧翼区域和1081bp 的3'侧翼区域选择作为pep2删除质粒的基础。侧翼区域片段使用表1.3中所 列的引物通过PCR产生。产物用琼脂糖凝胶电泳分离且正确的片段使用标准 实验室方法利用凝胶提取试剂盒(Qiagen)从凝胶分离。用于侧翼区域的PCR 中的模板DNA来自里氏木霉野生型菌株QM6a。bar盒用NotI消化从pTTv41 获得。载体骨架是EcoRI/XhoI消化的pRS426，如用于以上的pTTv41。质粒 使用以上对于pTTv41描述的酵母同源重组方法构建。这一用于pep2的删除 质粒(pTTv96)导致pep2基因座中的1437bp删除并覆盖PEP2的完整编码序 列。
表1.3:用于产生pep2删除质粒的引物
产生利妥昔单抗的pep2删除菌株M455的产生
为去除载体序列，质粒pTTv96(Δpep2-bar)用PmeI消化且正确的片段使 用QIAquick凝胶提取试剂盒(Qiagen)从琼脂糖凝胶纯化。大约6μg的pep2 删除盒用于转化菌株M169(表达调和的利妥昔单抗抗体)。原生质体的制备和 转化使用bar选择如以上对于M182描述的进行。
200个以上的克隆挑取作为选择性划线。29个转化体作为第二划线生长 良好。作为选择性划线快速生长的最好的10个转化体使用标准实验室方法利 用表1.4中所列的引物通过PCR筛选正确的整合。删除盒正确地整合到分析 的10个克隆中的9个中。开放阅读框通过PCR在分析的10个转化体的9个 中删除。五个破坏体纯化为单细胞克隆。一个纯转化体(206A)以菌株编号
M455命名。
表1.4:用于筛选pep2删除构建体的整合的引物
用于筛选pTTv96的整合
用于筛选pep2 ORF的删除
产生利妥昔单抗的pep2删除菌株的分析
M455菌株、4个另外的pep2删除转化体和亲本利妥昔单抗产生菌株 M169在28℃下补充20g/l废谷物提取物、40g/l乳糖、100mM PIPPS和8.1 g/l酪蛋白氨基酸的木霉属最低培养基(TrMM)pH 5.5中的摇瓶培养物中生长。 为分析对于利妥昔单抗产生的影响，来自第5天培养样品的30μl上清液进行 免疫印迹。重链用TBST中1:10,000稀释的抗重链AP偶联抗体(Sigma#A3188) 检测。轻链用抗-κ轻链AP偶联抗体(Sigma#A3813)检测。重链产生的轻微提 高在转化体206A中看到(图6)。重链片段化，但全长和38kD片段相比于 亲本菌株上略微提高。另外，总蛋白酶活性按照制造商的方案用琥珀酰化酪 蛋白(QuantiCleave蛋白酶分析试剂盒,Pierce#23263)测量。转化体206A/M455 与亲本菌株M169活性相比显示出蛋白酶活性的最大降低(图7)。上清液中的 总蛋白酶活性对于M455降低10％。
毕赤酵母表达的天冬氨酸蛋白酶的分析
毕赤酵母表达的里氏木霉天冬氨酸蛋白酶pep3(tre121133)和pep7 (tre58669)也通过测量MAB01抗体和IGF-1的降解来体外测试。MAB01和 IGF-1被pep3和pep7降解通过免疫印迹进行分析。天冬氨酸蛋白酶在毕赤酵 母上清液中产生。毕赤酵母上清液稀释到lx浓度，并随后与50mM柠檬酸 钠缓冲液，pH 5.5混合。MAB01添加到各反应中以使得最终浓度是0.05μg/μl。 IGF-1添加到各反应中以使得最终浓度是0.30μg/μl。然后采样10微升的各反应混合物并添加到具有β-巯基乙醇的3μl Laemmli样品缓冲液中。样品在 与所有蓝色精确plus预染色分子量标志物(BioRad)一起加载到4-15％PAGE 凝胶(BioRad mini-proteanTGX预制凝胶)中之前在95℃下加热5分钟。PAGE 凝胶在200V下运行30分钟。凝胶中的蛋白质然后在100V下1小时电转移 到硝基纤维素滤膜中。含蛋白质的硝基纤维素滤膜然后用具有0.1％吐温的 Tris缓冲盐水(TBST)中的5％乳粉封闭，在室温下振摇1小时。封闭的膜然后 用抗体探测。含MAB01的膜用TBST中1:30,000稀释的抗IgG重链抗体AP 偶联物(Sigma#A3188)探测。IGF-1样品使用一级抗IGF-1抗体(TBST中 1:2000)和抗IgG AP偶联的次级抗体(TBST中1:5000)分析。所有抗体孵育在 搅拌器上室温下进行1小时。膜然后在搅拌器上用TBST洗涤3次，各20分 钟。膜用BCIP/NBT碱性磷酸酶底物(Promega#S3771)显影最多5分钟。如图 8中显示的，pep3蛋白酶在37℃下孵育过夜后在pH 5.5下具有低MAB01降 解活性，但在pH 4.5下活性较高。pep7蛋白酶在pH 4.5下仅具有最低抗体降 解活性。
使用SIP肽分离另外的天冬氨酸蛋白酶
几种另外的天冬氨酸蛋白酶从里氏木霉M277三重蛋白酶删除菌株(pep1, tsp1,slp1)分离。M277菌株不表达异源蛋白质。M277删除菌株如以下实施例 4的描述产生。菌株在28℃下补充20g/l废谷物提取物、60g/l乳糖和9g/l 酪蛋白氨基酸的木霉属最低培养基pH 5.5中生长。天冬氨酸蛋白酶使用SIP 肽(Ac-Phe-Lys-Phe-(AHPPA)-Leu-Arg-NH2)(Kataoka Y.等2005FEBS Letters 579,pp 2991-2994)通过亲和纯化分离。SIP肽使用制造商提供的方案与NHS 活化的琼脂糖树脂(Pierce#26196)偶联。SIP亲和树脂用于纯化蛋白酶。来自 里氏木霉M277菌株的发酵上清液(15ml)用于将蛋白酶分批结合在35ml含 50mM柠檬酸钠、0.2M NaCl的缓冲液，pH 3.0中的树脂(来自发酵条件pH 5.5；28℃；9g/l酪蛋白氨基酸；20g/L废谷物提取物、60g/L乳糖)。柱用相 同的结合缓冲液洗涤并且结合的蛋白质用洗脱缓冲液(50mM Tris-HCL、1M NaCl，pH 8.5)除去。然后收集0.5ml的级分。
30μl的各纯化级分然后在4-15％SDS PAGE凝胶(BioRad mini-protean TGX预制凝胶)上运行并用GelCode蓝(Thermo Scientific)染色过夜。SDS PAGE凝胶显示约42kDa的主条带和约25kD的微弱条带(图9)。来自凝胶的 条带然后剪切并用测序级的修饰胰蛋白酶(Promega#V5111)进行凝胶内胰蛋 白酶消化。所得的肽从凝胶提取并通过C18 ZipTip(Millipore#ZTC18M096) 纯化。纯化的肽在QSTAR Pulsar,ESI-混合四极-TOF(AB Sciex)上通过 LC-MS/MS分析。这一分析揭示，PEP2、PEP3、PEP4和PEP5与GAP1和 SLP2一起存在于样品中。据信，约25kD的弱条带对应于谷氨酸蛋白酶GAP1。
SIP纯化的蛋白酶然后测试其降解MAB01抗体重链的能力。纯化的SIP 蛋白酶在37℃下与柠檬酸钠缓冲液中终浓度0.05μg/μl的MAB01孵育过夜。 样品在pH 4.0和pH 5.5下孵育，且都在SIP抑制肽存在或不存在的情况下。 反应在之后采样。收集的样品通过用TBST中1:30,000稀释的抗IgG重链抗 体AP偶联物(Sigma A3188)的免疫印迹进行分析。免疫印迹的结果表明蛋白 酶在pH 4.0下孵育时具有针对MAB01重链的高的蛋白酶活性，且在pH5.5 下具有降低的活性(图10)。另外，天冬氨酸蛋白酶和谷氨酸蛋白酶活性两者 通过与SIP肽一起孵育受到抑制(图10)。
SIP-纯化的天冬氨酸蛋白酶的分析
蛋白酶活性然后在蛋白酶抑制剂存在和不存在的条件下针对酪蛋白进行 测试。针对酪蛋白的蛋白酶活性使用EnzChek蛋白酶分析试剂盒(Molecular probes#E6638，绿色荧光酪蛋白底物)测试。工作原液通过在50mM柠檬酸 钠，pH 5.5中将原液稀释到10μg/ml制备。纯化的蛋白酶级分(10μl)用40μl 的柠檬酸钠，pH 5.5稀释。100μl稀释的底物在96孔样品板中与稀释的蛋白 酶级分混合。板然后被覆盖并保持在37℃下一到三小时。在一、二和三小时 时用Varioskan荧光读板仪(Thermo Scientific)利用485nm激发和530nm发射 获取荧光读数。
SIP抑制肽、胃蛋白酶抑制剂A、LIP肽、SBTI和胰凝乳蛋白酶抑制剂 用作抑制剂。SIP抑制肽抑制天冬氨酸蛋白酶和谷氨酸蛋白酶两者；胃蛋白 酶抑制剂A仅抑制天冬氨酸蛋白酶；LIP肽仅抑制谷氨酸蛋白酶；SBTI能够 抑制SLP2和PEP4；和胰凝乳蛋白酶抑制剂抑制SLP2。SIP、LIP和胃蛋白 酶抑制剂A以60uM的浓度使用，和SBTI以200μg/ml的浓度使用，为区 分天冬氨酸蛋白酶和谷氨酸蛋白酶，胃蛋白酶抑制剂A用作抑制剂，因为其 不抑制谷氨酸蛋白酶。
当研究酪蛋白消化时，大部分的SIP蛋白酶活性被胃蛋白酶抑制剂A抑 制(图11)。酪蛋白降解研究的结果表明纯化的级分中pH 5.5下的大部分活性 来自天冬氨酸蛋白酶。LIP肽，其为GAP1前肽，与SIP抑制剂相比对蛋白 酶活性的抑制稍低。SBTI和胰凝乳蛋白酶抑制剂能够抑制纯化的样品中的 SLP2蛋白酶。
这些结果支持SIP级分中存在4种天冬氨酸蛋白酶(PEP2、PEP3、PEP4 和PEP5)的结论。
实施例2–谷氨酸蛋白酶的鉴定
这一实施例证明来自里氏木霉(Trichoderma reesei)培养上清液的谷氨酸蛋 白酶降解抗体重链和轻链的能力。
gap1删除的分析
先前已经确定在里氏木霉基因组中有四种谷氨酸蛋白酶序列。最丰富的谷 氨酸蛋白酶是gap1(tre69555)，如通过转录谱确定的。因此，gap1蛋白酶由 SIP肽亲和色谱从里氏木霉上清液纯化，如实施例1中描述的。
gap1删除然后使用里氏木霉MAB01抗体产生菌株M244(Δpep1)产生。
gap1删除质粒的产生
用于谷氨酸蛋白酶gap1(TreID69555)的删除pTTv117质粒基本上如实施 例1中对于pep1删除质粒pTTv41所述构建。1000bp的5'侧翼区域和1100bp 的3'侧翼区域选择作为gap1删除质粒的基础。侧翼区域片段使用表2.1所列 的引物通过PCR产生。产物用琼脂糖凝胶电泳分离且正确的片段使用标准实 验室方法利用凝胶提取试剂盒(Qiagen)从凝胶分离。用于侧翼区域的PCR中 的模板DNA来自里氏木霉野生型菌株QM6a。pyr4 blaster盒用NotI消化从 pTTv71获得。载体骨架如实施例1中是EcoRI/XhoI消化的pRS426。质粒使 用如实施例1中描述的酵母同源重组方法构建。这一用于gap1的删除质粒 (pTTv117)导致gap1基因座中的1037bp删除并覆盖GAP1的完整编码序列。
表2.1:用于产生gap1删除质粒的引物
用于gap1(TreID69555)的删除质粒pTTv117，载体骨架pRS426
产生MAB01的Δpep1Δgap1双重删除菌株M296的产生
为产生用于第二蛋白酶删除r MAB01抗体产生菌株，pep1删除菌株 M181(实施例1)采用潮霉素和乙酰胺选择用MAB01轻链和重链构建体 (pTTv98+pTTv67)转化。具有pep1删除的这一MAB01菌株以编号M244命 名。从pep1基因座去除pyr4 blaster盒基本上如以下实施例3中针对M195(在 双重蛋白酶删除菌株M219的产生中)所述进行。这一pyr4-菌株以编号M285 命名并用作后续蛋白酶删除的亲本。
为除去载体序列，质粒pTTv117(Δgap1-pyr4)用PmeI消化且正确的片段 使用QIAquick凝胶提取试剂盒(Qiagen)从琼脂糖凝胶纯化。大约5μg的gap1 删除盒用于转化菌株M285(MAB01抗体菌株M244的pyr4-，基于Δpep1菌 株M181)。原生质体的制备和转化使用pyr4选择基本上如实施例1中对于 pep1删除菌株M181和M195所述进行。
来自转化板的集落挑取作为选择性划线。作为选择性划线快速生长的克 隆使用标准实验室方法利用表2.2中所列的引物通过PCR筛选正确的整合。 推定的破坏体纯化为单细胞克隆。
表2.2:用于筛选gap1整合和菌株纯度的引物
用于筛选pTTv117的整合
用于筛选gap1 ORF的删除
产生MAB01的Δpep1Δgap1双重删除菌株的分析
双重删除菌株(Δpep1Δgap1)在含300ml的补充40g/l乳糖、20g/l废谷物 提取物和9g/l酪蛋白氨基酸并用100mM PIPPS缓冲到pH 5.5的木霉属最低 培养基的2升摇瓶中生长。Δgap1菌株然后针对MAB01重链和轻链产生进 行测试(图12)。Δgap1菌株与各具有slp1、slp2和slp3删除的菌株比较。Δpep1 菌株M244用作对照。样品来自第7天大摇瓶培养物。样品使用抗IgG重链 (Sigma#A3188)或抗轻链(Sigma#A3812)AP偶联物通过免疫印迹分析(图 12)。gap1删除导致与M244对照菌株相比重链产生2倍的提高和轻链产生 1.6倍的提高(图13)。
gap2删除的分析
基于从M194里氏木霉菌株产生的转录谱数据，第二丰富的谷氨酸蛋白 酶确定为gap2(tre106661)。因此，gap2蛋白酶也使用pTTV145删除构建体 从M244(Δpep1)删除。
gap2删除质粒的产生
用于谷氨酸蛋白酶gap2(TreID106661)的pTTv145删除质粒基本上如实 施例1中对于pep1删除质粒pTTv41描述的构建。1021bp的5'侧翼区域和 1010bp的3'侧翼区域选择作为gap2删除质粒的基础。在这一质粒中，pyr4 blaster盒的同向重复片段从pyr4 5'UTR改变为从gap2 5'侧翼区域的320bp 同向重复序列，且无AscI位点添加在pyr4和5'同向重复之间。这一类型的 blaster盒应在切除后对于删除基因的基因座不遗留任何额外的序列。片段使 用表2.3中所列的引物通过PCR产生。产物用琼脂糖凝胶电泳分离且正确的 片段使用标准实验室方法利用凝胶提取试剂盒(Qiagen)从凝胶分离。用于侧翼 区域的PCR中的模板DNA是里氏木霉野生型菌株QM6a。pyr4标记基因利 用NotI消化获自pHHO5。载体骨架是如实施例1中的EcoRI/XhoI消化的 pRS426。质粒使用如实施例1中描述的酵母同源重组方法构建。这一用于gap2 的删除质粒(pTTv145)导致gap2基因座中的944bp删除并覆盖GAP2的完整 编码序列。
表2.3:用于产生gap2删除质粒的引物
用于gap2(TreID106661)的删除质粒pTTv145，载体骨架pRS426
产生MAB01的Δpep1Δgap2双重删除菌株M360的产生
为产生用于第二蛋白酶删除的MAB01抗体产生菌株，pep1删除菌株 M181(实施例1)采用潮霉素和乙酰胺选择用MAB01轻链和重链构建体 (pTTv98+pTTv67)转化。pyr4blaster盒从pep1基因座的去除基本上如以下实 施例3针对M195(在双重蛋白酶删除菌株M219的产生中)所述进行。这一 pyr4-菌株以编号M285命名并用作后续蛋白酶删除的亲本。
为除去载体序列，质粒pTTv145(Δgap2-pyr4)用PmeI消化且正确的片段 使用QIAquick凝胶提取试剂盒(Qiagen)从琼脂糖凝胶纯化。大约5μg的gap2 删除盒用于转化菌株M285(MAB01抗体菌株M244的pyr4-，基于Δpep1菌 株M181)。原生质体的制备和转化使用pyr4选择基本上如实施例1中对于菌 株M181和M195所述进行。
来自转化板的集落挑取作为选择性划线。作为选择性划线快速生长的克 隆使用标准实验室方法利用表2.4中所列的引物通过PCR筛选正确的整合。 推定的破坏体纯化为单细胞克隆。
表2.4:用于筛选gap2整合和菌株纯度的引物
用于筛选pTTv145的整合
用于筛选gap2 ORF的删除
产生MAB01的Δpep1Δgap2双重删除菌株的分析
产生了几种转化体。来自这些转化体的培养上清液在4-15％SDS PAGE 凝胶上运行并然后MAB01抗体重链用抗重链AP偶联抗体(Sigma#A3188)通 过免疫印迹分析，且轻链用抗轻链AP偶联抗体(Sigma#A3812)检测。免疫印 迹的结果显示删除gap2导致MAB01重链和轻链的产生提高数倍(图14)。
毕赤酵母表达的gap2的分析
包含里氏木霉gap2的毕赤酵母上清液也在体外进行研究。含gap2的上 清液和MAB01抗体稀释到调节为pH 4.0、4.5、5.0和5.5的柠檬酸钠缓冲液 中，并在37℃下孵育20小时。样品在0分钟时和20小时后获取。MAB01 重链产生使用抗IgG重链(Sigma#A3188)抗体AP偶联物通过免疫印迹分析。 免疫印迹的结果显示gap2在pH 4.0下具有针对重链MAB01的最大蛋白水解 活性(图15)。尽管gap2蛋白酶活性在pH 5.5下较低(图15)，经过4天它能够证明对重链的显著活性。gap2蛋白酶产生约25kD的降解产物，表明它在重 链铰链区中具有蛋白水解活性。
实施例3–丝氨酸蛋白酶的鉴定
这一实施例证明来自里氏木霉(T.reesei)的丝氨酸蛋白酶降解抗体重链和 轻链的能力。
丝氨酸蛋白酶的纯化
丝氨酸蛋白酶包括已鉴定为抗体降解酶的蛋白酶大家族。因此，丝氨酸 蛋白酶从木霉属上清液纯化。丝氨酸蛋白酶首先用p-氨基苄脒琼脂糖4快速 流树脂GE healthcare#17-5123-10)从发酵培养上清液纯化。15ml的发酵培养 上清液分批结合35ml结合缓冲液(0.05M Tris-HCL,0.5M NaCl,pH 7.4)中的 树脂。在填充和用相同的结合缓冲液洗涤柱后，柱用0.05M甘氨酸，pH 3.0 洗脱。然后级分用1M Tris HCL，pH 8.8中和。
总共1.7mg的蛋白质从亲和柱纯化。当峰级分在4-15％SDS-PAGE凝胶 上运行时，看到几个主条带(～110kD、53kD、39kD、29kD)和许多更小的条 带。峰级分蛋白质混合物(F4)然后通过将F4的样品与人IgG1在37℃下柠檬 酸钠缓冲液(50mM,pH 5.5)中孵育20小时测试蛋白酶活性。样品在存在和不 存在丝氨酸蛋白酶抑制剂PMSF(5mM)的两种情况下孵育。孵育的样品然后 用TBST中1:30,000稀释的抗IgG重链AP偶联抗体(Sigma#A3188)和抗IgG 轻链AP偶联抗体(Sigma#A3812)通过免疫印迹进行分析。免疫印迹的结果显 示F4纯化蛋白质级分完全降解IgG(图16)。另外，用PMSF处理能够抑制大 部分的降解，表明负责IgG降解的F4级分中的蛋白酶活性主要是丝氨酸蛋白 酶活性。
为了确定纯化的级分中哪些蛋白质表现出蛋白酶活性，峰级分在IgG(0.5 mg/mlMAB02)SDS PAGE酶谱凝胶(12％)上运行。纯化的级分和未纯化的上 清液样品在变性条件下在酶谱凝胶上运行。在运行凝胶后，凝胶中的蛋白质 通过将凝胶在1％triton X-100中孵育以除去SDS而复性。酶谱凝胶然后允许 在反应缓冲液(50mM柠檬酸钠,pH 5.5)中孵育过夜以使得蛋白酶可以降解凝 胶中的IgG。凝胶然后用GelCode蓝染色以显示IgG染色的程度。活性蛋白 酶产生清晰的条带而无染色(图17)。
IgG凝胶酶谱上存在约29kD和65kD的两个可见的清晰条带。但是， 29kD处的条带显著得多，表明它可能负责样品中的大多数丝氨酸蛋白酶活 性。这些条带是在未纯化上清液样品中仅有的两个可见条带，且在纯化的级 分中更显著(图17)。当蛋白酶样品用PMSF(已知的丝氨酸蛋白酶抑制剂) 预处理时，清晰的白色条带表现为灰色或不可见，表明该条带对应于丝氨酸 蛋白酶(图17)。
29kD丝氨酸蛋白酶TSP1的鉴定
从没有MAB02的匹配SDS PAGE凝胶中，29kD条带从凝胶剪切并用测 序级的修饰胰蛋白酶(Promega#V5111)进行凝胶内胰蛋白酶消化。在纯化的级 分中，29kD条带作为清楚的条带观察到。这一清楚的条带然后分离。所得 的肽从凝胶提取并通过C18 ZipTip(Millipore#ZTC18M096)纯化。纯化的肽在 QSTAR Pulsar,ESI-混合四极-TOF(AB Sciex)上通过LC-MS/MS分析。所得的 质量分析清楚地鉴定29kD条带为胰蛋白酶样丝氨酸蛋白酶TSP1(tre73897, 35％序列覆盖)。
tsp1删除的分析
编码TSP1的基因(tsp1)然后从利妥昔单抗抗体产生菌株M169删除以建 立M183(Δtsp1)。摇瓶培养用M169和tsp1删除菌株转化体进行以测量对利 妥昔单抗表达的影响。培养物在具有4g/L乳糖、2g/L废谷物提取物和100 mM PIPPS的300ml TrMM,pH 5.5中生长。第5天的上清液样品加载到4-15％ SDS PAGE凝胶中并转移到硝基纤维素用于用TBST中1:10,000稀释的抗重 链AP偶联抗体(Sigma#A3188)进行免疫印迹。两个tsp1删除菌株转化体与亲 本对照菌株相比显示利妥昔单抗重链表达的明显增加(图40)。
用于第一蛋白酶基因pep1(TreID74156)的删除构建体如在以上实施例1 中描述的设计。
tsp1删除质粒的产生
用于碱性胰蛋白酶样丝氨酸蛋白酶tsp1(TreID71322/TreID73897,Dienes 等,2007,Enz Microb Tech 40:1087-1094)的删除质粒基本上如实施例1中针对 pep1删除质粒所述构建。953bp的5'侧翼区域和926bp的3'侧翼区域选择作 为tsp1删除质粒的基础。如对于pep1一样，用于tsp1的第一删除质粒(pTTv42) 使用bar作为选择标记。侧翼区域片段使用表3.1中所列的引物通过PCR产 生。产物用琼脂糖凝胶电泳分离且正确的片段使用标准实验室方法利用凝胶 提取试剂盒(Qiagen)从凝胶分离。用于侧翼区域的PCR中的模板DNA来自里 氏木霉野生型菌株QM6a。bar标记用NotI消化获自pTTv41(实施例1)。载体 骨架是如实施例1中的EcoRI/XhoI消化的pRS426。质粒使用实施例1中所 述的酵母同源重组方法构建。
为克隆第二tsp1删除质粒(pTTv72)，bar标记用NotI消化从删除质粒 pTTv42去除。pyr4 blaster盒用NotI消化获自pTTv71(实施例1)，与NotI切 割的pTTv42连接并使用标准实验室方法转化到大肠杆菌中。培养几个转化 体，分离质粒DNA并消化以使用标准方法筛选pyr4 blaster盒的正确连接和 定向。一个具有正确插入序列大小和方向的克隆被测序和储存。这些用于tsp1 的删除质粒(pTTv42和pTTv72)导致tsp1基因座中的1252bp删除并覆盖TSP1 的完整编码序列。
表3.1:用于产生tsp1删除质粒的引物
用于tsp1(TreID71322/TreID73897)的删除质粒pTTv42，载体骨架pRS426
用于tsp1(TreID71322/TreID73897)的删除质粒pTTv72，载体骨架pTTv42
pep1tsp1双重删除菌株M219的产生
为再利用pyr4作为选择标记，pyr4 blaster盒从pep1删除菌株M195去 除。孢子展布到含20g/l乳糖、2g/l朊蛋白胨、1ml/l Triton X-100、5mM尿 苷和1.5g/l 5-FOA，pH 4.8的最低培养基板上。5-FOA抗性集落在5-7天后 挑取到0.9％NaCl，通过涡旋完全悬浮并通过棉花填充的吸液头过滤。为纯 化克隆为单细胞克隆，滤液再次如上所述展布到板上。纯化的克隆在包含39 g/l的土豆右旋糖琼脂糖的板上形成孢子。这些克隆通过将孢子涂布到最低培 养板(20g/l葡萄糖，1ml/l Triton X-100)上测试尿苷营养缺陷型，其中未观察 到生长，表明选择的克隆是pyr4-。所有克隆进一步通过PCR(使用表3.2中所 列的引物)测试blaster盒的去除并显示为正确。用于产生的双重蛋白酶删除菌 株(M219)的克隆(9-35A-lA-a)以菌株编号M196(Δpep1,pyr4-)命名。
为去除载体序列，质粒pTTv72(Δtsp1-pyr4)用PmeI消化且正确的片段使 用QIAquick凝胶提取试剂盒(Qiagen)从琼脂糖凝胶纯化。大约5μg的tsp1 删除盒用于转化M196(Δpep1,pyr4-)。原生质体的制备和转化使用pyr4选择 基本上如实施例1中对于pep1删除菌株M181和M195所述进行。
挑取超过100个集落且48个使用标准实验室方法利用表3.2中所列的引 物通过PCR筛选删除盒的正确整合和也筛选tsp1 ORF的删除。四个推定 Δtsp1克隆纯化为单细胞克隆。tsp1的删除使用如实施例1中对于M181和 M195所描述的标准实验室方法通过这些克隆的DNA印迹分析验证(图18A)。 DNA印迹分析也表明仅四个转化体(来自两个转化体的两个平行克隆，克隆 16-5AA、16-5BA、16-11AA、16-11BA，图18B和18C)是单一整合体，其 它克隆确定为在基因组中的其它地方携带另外的拷贝并放弃。为排除图18 中看到的对于转化体中tsp1 ORF的弱信号来源于tsp1基因，tsp1 ORF的删除 使用表3.2中所列的引物通过PCR证实。没有获得tsp1 ORF的信号。用于去 除pyr4 blaster盒(和产生三重删除菌株M277)的克隆(16-5AA)以菌株编号 M219(Δpep1Δtsp1)命名。
产生MAB01的Δpep1Δtsp1双重删除菌株M252的产生
为去除载体序列，质粒pTTv42(Δtsp1-bar)用PmeI消化且正确的片段使 用QIAquick凝胶提取试剂盒(Qiagen)从琼脂糖凝胶纯化。大约5μg的tsp1 删除盒用于转化菌株M181(Δpep1，实施例1)。原生质体的制备和转化基本 上如实施例中对于pep1删除菌株M182所述的使用bar选择进行。
在转化板上生长的集落挑取作为选择性划线。作为选择性划线快速生长 的克隆使用标准实验室方法利用表3.2中所列的引物通过PCR筛选正确的整 合。推定的破坏体纯化为单细胞克隆。Tsp1的删除使用如实施例1中对于 M181和M195所描述的标准实验室方法通过这些克隆的DNA印迹分析验证 (图19A)。所有克隆也验证为单一整合体(图19B和19C)。一个双重蛋白酶删 除克隆(13-172D)以编号M194命名。
表3.2:用于筛选pyr4 blaster盒的去除和用于筛选tsp1整合和菌株纯度的引 物
用于筛选pyr4 blaster盒从M195的去除
用于筛选pTTv42的整合
用于筛选pTTv72的整合
用于筛选tsp/ORF的删除
双重蛋白酶删除菌株M194用于产生如下的MAB01抗体表达菌株M247 和M252。菌株M247的构建通过用MAB01重链和轻链构建体(pTTv101+ pTTv102)转化M194来进行。菌株M252通过用MAB01重链和轻链构建体 (pTTv99+pTTv67)转化M194来构建。两种转化是基于潮霉素和乙酰胺选择。 产生MAB01的Δpep1Δtsp1双重删除菌株M252的分析
产生MAB01抗体的双重删除菌株(Δpep1Δtsp1)在发酵罐中生长时显示为 产生261mg/l抗体，具有43％全长抗体。菌株的蛋白酶活性然后通过使菌株 在22℃下在补充20g/l废谷物提取物、60g/l乳糖和9g/l酪蛋白氨基酸的木 霉属最低培养基pH 5.5中生长进行测试。这一菌株中针对酪蛋白的总蛋白酶 活性确定为比野生型M124菌株低2.0倍(图20)。
65kD丝氨酸蛋白酶SLP1的鉴定
产生约65kD的活性的蛋白酶由于其低表达水平和尺寸上与几种高表达 的蛋白质接近而更难于鉴别。高度表达的蛋白质先前鉴定为CBHI、CBHII、 CIP2和木聚糖酶4。进行改进以更好地将65kD蛋白酶与高度表达的蛋白质 分离。改进包括使用用于酶谱的较低凝胶百分比(7％)SDS PAGE凝胶和标准 SDS PAGE凝胶以运行样品更长时间以使得54kD分子量标志物在凝胶的底 部。另外，来自里氏木霉利妥昔单抗抗体转化体的发酵上清液也用于纯化丝 氨酸蛋白酶。利妥昔单抗抗体转化体是菌株M169，其产生利妥昔单抗且缺 乏蛋白酶删除。菌株在28℃下补充20g/l废谷物提取物和60g/l乳糖的木霉 属最低培养基pH 5.5中生长。这一培养中产生的CBHI缺乏纤维素结合结构 域，因此它小大约10kD。但是，M169不显示对应于65kD蛋白酶的清楚条 带(图21)。因此，切割一般区域并用测序级的修饰胰蛋白酶(Promega#V5111) 进行凝胶内胰蛋白酶消化。所得的肽从凝胶提取并通过C18 ZipTip(Millipore #ZTC18M096)纯化。纯化的肽在QSTAR Pulsar,ESI-混合四极-TOF(AB Sciex)上通过LC-MS/MS分析。
肽分析显示第二高得分的蛋白质是蛋白酶tre51365。最高得分的蛋白酶 是木聚糖酶4，其是样品中的污染物。tre51365枯草溶菌素蛋白酶(现在称为 SLP1)在来自三个单独纯化的3个独立样品中发现。在最佳得分的样品中， 发现6种肽并通过LC-MS/MS测序。序列覆盖率为8％，因为天然蛋白酶基 因编码构成93kD蛋白酶的882个氨基酸。在明胶酶谱分析中，～90kD的弱 条带可以与到65kD的弥散一起看到，表明SLP1蛋白酶本身发生蛋白水解但保持其活性的大部分。
slp1删除质粒的产生
SLP1编码基因(slp1)然后在产生MAB01抗体的菌株M244(Δpep1)中删 除。
用于枯草溶菌素样蛋白酶slp1(TreID51365)的删除质粒基本上如实施例 1中针对pep1删除质粒pTTv41所述构建。1094bp的5'侧翼区域和1247bp 的3'侧翼区域选择作为slp1删除质粒的基础。片段使用表3.3中所列的引物 通过PCR产生。产物用琼脂糖凝胶电泳分离且正确的片段使用标准实验室 方法利用凝胶提取试剂盒(Qiagen)从凝胶分离。用于侧翼区域的PCR中的模 板来自里氏木霉野生型菌株QM6a。pyr4 blaster盒用NotI消化自pTTv71(实 施例1)获得。载体骨架是如实施例1中的EcoRI/XhoI消化的pRS426。质粒 使用实施例1中所述的酵母同源重组方法构建。用于slp1的这一删除质粒 (pTTv126)导致slp1基因座中的2951bp删除并覆盖SLP1的完整编码序列。
表3.3:用于产生slp1删除质粒的引物
用于slp1(TreID51365)的删除质粒pTTv126，载体骨架pRS426
产生MAB01的Δpep1Δslp1删除菌株M298和M299的产生
为产生用于第二蛋白酶删除的MAB01抗体产生菌株，pep1删除菌株 M181(实施例1中)利用潮霉素和乙酰胺选择用MAB01轻链和重链构建体 (pTTv98+pTTv67)转化。pyr4blaster盒从pep1基因座的去除基本上如以上对 于M195所述(在双重蛋白酶删除菌株M219的产生中)进行。这一pyr4-菌 株以编号M285命名并用作后续蛋白酶删除的亲本。
为去除载体序列，质粒pTTv126(Δslp1-pyr4)用PmeI消化且正确的片段 使用QIAquick凝胶提取试剂盒(Qiagen)从琼脂糖凝胶纯化。大约5μg的slp1 删除盒用于转化M285(MAB01抗体菌株M244的pyr4-，基于Δpep1菌株 M181)。原生质体的制备和转化基本上如实施例中对于pep1删除菌株M181 和M195所述的使用pyr4选择进行。
在转化板上生长的集落挑取作为选择性划线。作为选择性划线快速生长 的克隆使用标准实验室方法利用表3.4中所列的引物通过PCR筛选正确的整 合。推定的破坏体纯化为单细胞克隆。
表3.4:用于筛选slp1整合和菌株纯度的引物
用于筛选pTTv126的整合
用于筛选slp1 ORF的删除
产生MAB01的Δpep1Δslp1双重删除菌株M298/M299的分析
M244菌株中slp1的删除显示预期的重链和轻链产生的提高(图12和13)。 slp1删除菌株(Δpep1Δslp1)在包含300ml的补充40g/l乳糖、20g/l废谷物提 取物和9g/l酪蛋白氨基酸并用100mM PIPPS缓冲到pH 5.5的木霉属最低培 养基的2升摇瓶培养中生长。如以上实施例2中所述，培养上清液在4-15％ PAGE凝胶上运行并进行免疫印迹以检测MAB01重链和轻链。重链以比 M244亲本菌株的产生水平高2.8倍的水平产生(图13)。轻链以比M244亲本 菌株的产生水平高1.8倍的水平产生(图13)。
另外的丝氨酸蛋白酶的鉴定
另外的抗体降解丝氨酸蛋白酶使用其它亲和配体鉴定。大豆胰蛋白酶抑 制剂(SBTI)有效地稳定抗体重链和轻链。因此，它能够抑制负责切割抗体的 蛋白酶。因此，为了鉴定这些蛋白酶，亲和纯化用与琼脂糖(Sigma#T0637) 偶联的SBTI进行。
里氏木霉菌株M44用于鉴定蛋白酶。M44菌株是没有异源蛋白表达的野 生型菌株。M44菌株在22℃下在补充20g/l废谷物提取物和60g/l乳糖的木 霉属最低培养基pH 5.5中生长。来自217小时样品的20ml M44培养上清液 样品与30ml结合缓冲液(50mM Tris,0.5MNaCl,pH 7.5)(pH 5.5；28℃；20 g/L废谷物提取物，60g/L乳糖)中的SBTI-琼脂糖亲和树脂(1ml)孵育。上清 液结合缓冲液混合物在50ml圆锥管中组合并在室温下搅拌1小时。混合物然后加添加到玻璃柱上并用200ml结合缓冲液洗涤。50ml的高盐混合物(1M NaCl)接着用于进一步除去非特异性相互作用。最后，柱再用100ml的原结 合/洗涤缓冲液洗涤。柱然后用50mM Tris,pH 5.0中的0.8M苄脒HC1洗脱。 级分以0.5ml体积收集并使用BioRad Bradford试剂以牛免疫球蛋白作为标准 进行蛋白质分析。
从收集的所有级分，190μg的蛋白质从SBTI亲和柱纯化。峰级分在具 有10kD分子量截止值的vivaspin超滤旋转过滤器(Sartorius-stedim)中洗涤以 除去苄脒抑制剂和浓缩该级分。浓缩的级分(cf3和cf4)和未浓缩的级分(fl-f4) 加载到MAB02酶谱凝胶(如上所述的)和常规SDS PAGE凝胶上用于分析。 酶谱的结果显示存在两种可见的蛋白水解活性(图22)。最显著的条带在40kD 附近可见且较弱条带在26kD附近可见(图22)。在酶谱凝胶中，较暗染色的 蛋白质条带侧邻白色酶谱活性条带。将其与加载到SDS PAGE凝胶上的浓缩级分比较，这些双峰条带可以在38kD附近看到(图23)。PAGE凝胶是4-15％ 梯度凝胶且酶谱凝胶是12％，因此相对大小可以略微不同。在PAGE凝胶上， 蛋白质条带可以清楚地在26kD的区域中看到，其对应于第二弱的酶谱活性 的大小。
为进一步分析cf3的纯化蛋白酶的蛋白水解活性，测试了级分降解利妥 昔单抗抗体重链的能力。5μl的cf3样品在柠檬酸钠缓冲液，pH 5.5中与0.05 μg/ml利妥昔单抗一起孵育。孵育的样品然后使用TBST中1:30,000稀释的抗 -人IgG重链特异性AP偶联抗体(Sigma#A3188)通过免疫印迹进行分析。免 疫印迹的结果显示蛋白酶立即降解利妥昔单抗抗体重链。全长利妥昔单抗重 链恰在50kD之上，而初始降解产物在45kD附近(图24)。另外，孵育过夜 产生38kD的另外的产物(图24)。
造成酶谱活性的蛋白酶在LC-MS/MS肽测序后鉴定。含蛋白质凝胶节段 从图23中显示的SDS PAGE凝胶切除并用测序级的修饰胰蛋白酶(Promega #V5111)进行凝胶内胰蛋白酶消化。所得的肽从凝胶提取并通过C18 ZipTip (Millipore#ZTC18M096)纯化。纯化的肽在QSTAR Pulsar,ESI-混合四极-TOF (AB Sciex)上通过LC-MS/MS分析。
最高得分的蛋白酶命中是枯草溶菌素样蛋白酶，slp2(tre123244)。发现 来自slp2的两个肽并测序，覆盖整个序列长度的6％。全长slp2蛋白酶是58 kD，但通常活性蛋白酶大小可能较小。
还存在邻近区域中发现的其它蛋白酶。较低26kD区域的分析鉴定了胰 蛋白酶丝氨酸样蛋白酶tsp1(tre73897)。这对应于观察到的微弱酶谱活性。如 上所述，这种蛋白酶通过氨基苄脒亲和纯化鉴定。
另外，全部的SBTI亲和纯化级分在溶液中进行胰蛋白酶消化以确定样 品的整个蛋白酶含量。其它鉴定的蛋白酶包括tre123865蛋白酶slp7(60kD)、 tre77579蛋白酶pep4(42kD)和tre58698蛋白酶slp8(41kD)。
里氏木霉枯草溶菌素蛋白酶slp5、slp6和slp7在毕赤酵母上清液中过度 产生用于研究其针对抗体利妥昔单抗和MAB01重链的活性(图25)。利妥昔 单抗模拟上清液(mocksupernatant)与含slp5和slp6的上清液比较(图25A)。 MAB01模拟上清液与含slp7的上清液比较(图25B)。利妥昔单抗和MAB01 添加到含蛋白酶的毕赤酵母上清液中并在37℃下孵育过夜。获取样品并通过 抗-重链AP偶联抗体的免疫印迹进行分析。这一分析揭示，与模拟对照上清 液相比，slp6蛋白酶显示利妥昔单抗重链的强降解活性和MAB01的弱降解 (图25)。
slp2和slp3删除质粒的产生
基于上述结果，slp2和slp3蛋白酶基因各自从MAB01抗体产生菌株 M244删除。
枯草溶菌素样蛋白酶slp2(TreID123244)和slp3(TreID 123234)的删除质 粒基本上如实施例1中对于pep1删除质粒pTTv41所述构建。1000bp的5' 侧翼区域和1100bp的3'侧翼区域选择作为slp2删除质粒的基础。对于slp3， 选择1000bp的5'侧翼区域和1100bp的3'侧翼区域。使用表3.5中所列的引 物通过PCR产生片段。产物使用琼脂糖凝胶电泳分离且正确的片段使用标准 实验室方法利用凝胶提取试剂盒(Qiagen)从凝胶分离。侧翼区域的PCR中使 用的模板来自里氏木霉野生型菌株QM6a。pyr4 blaster盒利用NotI消化从pTTv71(实施例1)获得。载体骨架是如实施例1中的EcoRI/XhoI消化的 pRS426。质粒使用如实施例1中描述的酵母同源重组方法构建。用于slp2的 删除质粒(pTTv115)导致slp2基因座中的2114bp删除并覆盖SLP2的完整编 码序列。用于slp3的删除质粒(pTTv116)导致slp3基因座中的1597bp删除并 覆盖SLP3的完整编码序列。
表3.5：用于质粒的引物
用于slp2(TreID123244)的删除质粒pTTv115，载体骨架pRS426
用于slp3(TreID123234)的删除质粒pTTv116，载体骨架pRS426
产生MAB01的Δpep1Δslp2和Δpep1Δslp3删除菌株M292和M295的产生
为产生用于第二蛋白酶删除的MAB01抗体产生菌株，pep1删除菌株 M181(在实施例1中)用潮霉素和乙酰胺选择以MAB01轻链和重链构建体 (pTTv98+pTTv67)转化。pyr4blaster盒从pep1基因座去除基本上如以上对于 M195所述(在双重蛋白酶删除菌株M219的产生中)进行。这一pyr4-菌株 以编号M285命名并用作用于后续蛋白酶删除的亲本。
为移除载体序列，质粒pTTv115(Δslp2-pyr4)和pTTv116(Δslp3-pyr4)用 PmeI消化并且正确的片段使用QIAquick凝胶提取试剂盒(Qiagen)从琼脂糖凝 胶纯化。大约5μg的任一删除盒用于单独地转化M285(MAB01抗体菌株 M244的pyr4-，基于Δpep1菌株M181)。原生质体的制备和转化基本上如实 施例1对于pep1删除菌株M181和M195所述的使用pyr4选择进行。
在转化板上生长的集落挑取作为选择性划线。作为选择性划线快速生长 的克隆采用标准实验室方法通过使用表3.6中所列的引物的PCR筛选正确整 合。推定的转化子纯化为单细胞克隆。甚至在重复的纯化步骤后没有获得纯 克隆。
表3.6：用于筛选slp2(pTTv115)和slp3(pTTv116)整合和菌株纯度的引物。
用于筛选pTTv115的整合
用于筛选slp2 ORF的删除
用于筛选pTTv116的整合
用于筛选slp3 ORF的删除
产生MAB01的Δpep1Δslp2和Δpep1Δslp3双重删除菌株M292和M295的分 析
M292菌株(Δpep1Δslp2)和M295菌株(Δpep1Δslp3)与其姊妹转化体一起 在包含300ml的补充有40g/l乳糖、20g/l废谷物提取物和9g/l酪蛋白氨基 酸并用100mM PIPPS缓冲到pH 5.5的木霉属最低培养基的2升摇瓶培养中 生长。培养上清液在4-15％SDS PAGE凝胶上运行并进行免疫印迹分析以检 测MAB01重链和轻链。结果显示两种删除都提高MAB01稳定性(图12和 13)。Δslp2删除在第7天提高摇瓶培养中MAB01重链表达约2.4倍，如与亲 本M244菌株相比的(图13)。Δslp3提高大摇瓶中MAB01重链表达约1.5 倍和提高MAB01轻链表达约1.7倍，如与M244亲本菌株相比的(图13)。 此外，当与Δslp3和Δgap1相比，Δslp2显示出相对于M244亲本菌株中MAB01 重链表达的最高倍数的MAB01重链表达提高(图13)。
当slp2从M306多重删除菌株(Δpep1Δtsp1Δslp1)删除时，slp2的删除导 致与亲本菌株相比孢子形成的减少和较慢的生长。
实施例4—木霉属多重蛋白酶删除菌株
本实施例证明以上实施例1-3中鉴定的包含蛋白酶基因的多重删除的里 氏木霉(T.reesei)菌株的提高的抗体产生和稳定性。
三重删除菌株Δpep1Δtsp1Δslp1的产生
产生了具有三重删除Δpep1Δtsp1Δslp1的里氏木霉菌株并测试抗体产生 的提高。该菌株也用于进一步的蛋白酶删除。
三重蛋白酶删除菌株M277的产生
为产生无标记的三重蛋白酶删除菌株，pyr4标记的环出基本上如以上对 于从单一蛋白酶删除菌株Δpep1环出pyr4所述应用于菌株M219。完成三个 相继的5-FOA选择步骤以确保所选择的克隆源自单一细胞。最终克隆通过 PCR(使用表3.1中所列的引物)验证pyr4的环出；用与pyr4的环出部分退 火的引物没有观察到特异性信号。环出进一步通过在有或没有5mM尿苷的 情况下将克隆接种到最低培养板上验证。用于产生三重蛋白酶删除菌株的克 隆以菌株编号M228(Δpep1Δtsp1,pyr4-)命名。
用于第三蛋白酶基因，枯草溶菌素样蛋白酶slp1(TreID51365)的删除质 粒pTTv126如上所述(表3.3)。这一删除质粒导致slp1基因座中的2951bp 删除并覆盖SLP1的完整编码序列。
为移除载体序列，质粒pTTv126(Δslp1-pyr4环出)用PmeI消化并且正确 的片段使用QIAquick凝胶提取试剂盒(Qiagen)从琼脂糖凝胶纯化。大约5μg 的slp1删除盒用于转化以上M228(Δpep1Δtsp1,pyr4-)。原生质体的制备和转 化基本上如实施例1中对于菌株M181和M195所述的使用pyr4选择进行。
200个克隆挑取作为第一划线。这些划线中的48个采用标准实验室方法 通过使用表4.1中所列的引物的PCR筛选正确整合。五个推定的三重蛋白酶 破坏子(Δpep1Δtsp1Δslp1)纯化为单细胞克隆。Slp1的删除通过五个克隆的 DNA印迹分析确认(图26A)。如实施例1中所述进行DNA印迹分析。DNA 印迹分析也确认三个克隆是单一整合体(图26B和26C)。两个另外的克隆显 示为基因组中其它地方携带另外的拷贝并放弃。用于去除pyr4blaster盒(且 为产生以下四重蛋白酶删除菌株M307)的克隆用菌株编号M277 (Δpep1Δtsp1Δslp1)命名。
表4.1：用于筛选pyr4 blaster盒的去除和用于筛选slp1整合和菌株纯度的引物。
用于筛选pyr4 blaster盒从M219的去除
用于筛选pTTv126的整合
用于筛选slp1 ORF的删除
产生MAB01的三重蛋白酶删除菌株M304的产生
为产生用于第三蛋白酶删除的MAB01抗体产生菌株，pep1/tsp1双重蛋 白酶删除菌株M194(实施例3)用潮霉素和乙酰胺选择以MAB01轻链和重 链构建体(pTTv99+pTTv67)转化。这种具有pep1/tsp1双重删除的MAB01菌 株用编号M252命名。pyr4 blaster盒从pep1基因座的去除基本上如实施例3 中对于M195(在双重蛋白酶删除菌株M219的产生中)所述进行。这种pyr4-菌株用编号M284命名并用作用于后续蛋白酶删除的亲本。
M284的第三蛋白酶删除通过使用slp1删除构建体pTTv128获得。这一 构建体包含靶向于slp1基因座的原始KEX2过表达盒。转化基本上按照实施 例1中对于菌株M181和M195描述的方案使用pyr4选择进行。所得的菌株 是MAB01产生三重蛋白酶删除菌株M304。
产生MAB01的三重蛋白酶删除菌株M304的分析
三重蛋白酶删除(Δpep1Δtsp1Δslp1)MAB01抗体产生菌株M304显示为 在培养基(pH 5.5；28->22℃；60g/L废谷物，30g/L葡萄糖，60g/L乳糖+ 乳糖补料)中以最高3.5g/L的产率产生MAB01抗体及最高84％的全长IgG 的产品质量(见下面的实施例6)。菌株的蛋白酶活性也通过在补充有60g/l 固体废谷物、30g/L葡萄糖和60g/L乳糖的木霉属最低培养基，pH 5.5中生 长进行测试。培养物在30℃下生长并随后对于生长相切换到22℃。分批补料培养用乳糖补料进行。这一菌株中针对酪蛋白的总蛋白酶活性测定为与野 生型菌株M124相比低约3.2倍(图20)。
单一、双重和三重删除菌株的比较
比较了来自单蛋白酶删除(Δpep1)菌株M181(参见实施例1)、双重蛋白酶 删除(Δpep1Δtsp1)菌株M219(参见实施例3)和三重蛋白酶删除 (Δpep1Δtsp1Δslp1)菌株M277的培养上清液的相对蛋白酶活性。这些删除菌 株与野生型菌株M124比较。三种蛋白酶删除菌株在具有300ml包含40g/l 乳糖、20g/l废谷物提取物和100mM PIPPS的TrMM，pH 5.5的2升摇瓶中 生长。样品在第3、5、7和10天获取。来自M124、M181、M219和M277 的第7天培养上清液样品各在柠檬酸钠缓冲液(50mM,pH 5.5)中1:2稀释，且 30μl加载到包含MAB02的12％酶谱SDS PAGE凝胶上。SDS PAGE凝胶在 100V下运行45分钟。凝胶然后在2.5％TritonX-100中孵育一小时，之后用 反应缓冲液(50mM柠檬酸钠,pH 5.5)洗涤几次。然后使酶谱凝胶在反应缓冲 液中振摇过夜。第二天早晨，凝胶用GelCode Blue染色试剂染色。其中MAB02抗体已经降解的区域在蓝染凝胶上显示为白色斑点。
两种蛋白酶活性在对照M124和M181 Δpep1样品中看到(图27)。最占优 势的活性在65-90kD之间看到，其对应于slp1。较微弱活性在28kD附近看 到，其对应于tsp1。如所预期的，M219 Δpep1Δtsp1菌株不产生28kD处的 酶谱条带。同样地，M277 Δpep1Δtsp1Δslp1菌株不产生任一酶谱活性。slp1 的活性大小看起来是可变的，因为在它在切割到65kD时仍然是活性的，即 使其成熟大小是90kD。尺寸变异可以在图27中看到。
来自M181、M219和M277删除菌株的上清培养的针对琥珀酰化酪蛋白 的总蛋白酶活性也从第3天、第5天和第7天样品测量。上清液在分析之前 首先在50mM柠檬酸钠,pH 5.5中稀释到2mg/ml总蛋白。50μl的稀释上清 液加载到96孔板中并添加50μl的琥珀酰化酪蛋白以开始反应。具有缓冲液 而不是酪蛋白的背景对照用于各样品。在添加酪蛋白后，允许蛋白酶反应在37℃下进行1小时。为发生反应，50μl的TNBSA试剂添加到各孔且板在 37℃下孵育16小时。对于整个板测量450nm处的吸光度。非特异性背景信 号从特异性蛋白酶活性测量值减去。如图28中所示，来自三个蛋白酶删除菌 株的上清液样品比M124野生型菌株低的蛋白酶活性。
来自M277和M124培养的上清液(第5和7天)在50mM柠檬酸钠缓冲 液中稀释到6mg/ml。向这些稀释的上清液中掺入MAB01抗体达到0.05μg/μl 的终浓度。这些反应在37℃下孵育过夜。在零时、1小时和过夜孵育时对反 应取样。20μl样品加载到4-15％SDS PAGE凝胶中并以200伏运行40分钟。 凝胶以100伏1小时转移到硝基纤维素上用于免疫印迹。膜用TBST中的5％ 乳封闭一小时。MAB01的重链用在TBST中1:30,000稀释的抗重链AP偶联 抗体(Sigma#A3188)检测。在用TBST洗涤膜后，印迹用AP底物(Promega) 显影。与过夜孵育的样品比较，特别明显的是重链在M124菌株上清液中更 多降解。M124不包含蛋白酶删除。利用3个蛋白酶删除，M277菌株产生显 著稳定的MAB01重链。在第5天，在孵育过夜后M277上清液中存在2.5倍 多的重链。对于第7天的上清液，存在4倍多的可见重链(图41)。
四重删除菌株M307
产生具有四重删除Δpep1Δtsp1Δslp1Δgap1的M307菌株并用于进一步的 蛋白酶删除。
四重蛋白酶删除菌株M307的产生
为产生无标记的四重蛋白酶删除菌株，pyr4 blaster盒的去除基本上如实 施例3中对于从单一蛋白酶删除菌株M195(Δpep1)去除pyr4 blaster盒所述应 用于菌株M277。完成三个相继的5-FOA选择步骤以确保所选择的克隆源自 单一细胞。最终克隆采用标准实验室方法通过用表4.2中所列的引物的PCR 验证blaster盒的去除。通过与pyr4的去除部分退火的引物未看到特异性信号。 去除进一步通过在有或没有5mM尿苷的情况下将克隆接种到最低培养基板 上验证。在无尿苷补充的板上没有观察到生长。用于产生四重蛋白酶删除菌 株的克隆以菌株编号M306(Δpep1Δtsp1Δslp1,pyr4-)命名。
用于第四蛋白酶基因(谷氨酸蛋白酶gap1(TreID69555))的删除质粒 pTTv117描述于实施例2中(表2.1)。这一删除质粒导致gap1基因座中的1037 bp删除并覆盖GAP1的完整编码序列。
为移除载体序列，质粒pTTv117(Agap1-pyr4)用PmeI消化并且正确的片 段使用QIAquick凝胶提取试剂盒(Qiagen)从琼脂糖凝胶纯化。大约5μg的 gap1删除盒用于转化以上M306(Δpep1Δtsp1Δslp1,pyr4-)。原生质体的制备 和转化基本上如实施例1中对于菌株M181和M195所述的使用pyr4选择进 行。
150个克隆挑取作为第一划线。这些划线中的48个采用标准实验室方法 通过使用表4.2中所列的引物的PCR筛选正确整合。八个推定的四重蛋白酶 破坏子(Δpep1Δtsp1Δslp1Δgap1)纯化为单细胞克隆。gap1的删除通过八个克 隆的DNA印迹分析确认(图29A)。如实施例1中所述进行DNA印迹分析。 DNA印迹分析也确认三个克隆是单一整合体(图29B和29C)。五个另外的克 隆显示为在基因组中其它地方携带另外的拷贝并放弃。用于去除pyr4blaster 盒(且为产生以下五重蛋白酶删除菌株M369)的克隆用菌株编号M307 (Δpep1Δtsp1Δslp1Δgap1)命名。
表4.2：用于筛选pyr4 blaster盒的去除和用于筛选gap1整合和菌株纯度的引 物
(49)用于筛选pyr4 blaster盒从M277的去除
用于筛选pTTv117的整合
用于筛选gap1 ORF的删除
产生MAB01的四重蛋白酶删除菌株M371的产生
为产生具有MAB01抗体产生的四重蛋白酶删除菌株，pyr4 blaster盒从 菌株M304的slp1基因座的去除基本上如实施例3中对于M195(在双重蛋 白酶删除菌株M219的产生中)所述进行。这种pyr4-菌株用编号M317命名 并用作用于后续蛋白酶删除的亲本。
对M317的第四蛋白酶删除通过使用以上gap1删除构建体pTTv117获 得。转化基本上按照实施例1中对于菌株M181和M195描述的方案使用pyr4 选择进行。所得的菌株是MAB01产生四重蛋白酶删除菌株M371。
四重蛋白酶删除菌株的分析
来自四重删除菌株M307的培养上清液的总蛋白酶活性然后测量并与来 自三重删除菌株M277和野生型菌株M124的培养上清液比较。各菌株在具 有300ml包含40g/l乳糖、20g/l废谷物提取物和100mM PIPPS的TrMM， pH 5.5的2升摇瓶中生长。获取第7天上清液样品用于总蛋白酶分析。上清 液的总蛋白浓度使用BCA分析以牛免疫球蛋白作为标准进行测量。上清液在 柠檬酸钠缓冲液，pH 5.5中系列稀释。稀释的上清液添加到荧光标记的酪蛋白底物并在37℃下孵育。荧光在1小时后以485nm和530nm发射测量。结 果显示，三重删除菌株M277的蛋白酶活性率比野生型菌株M124低3倍和 四重删除菌株M307比野生型菌株低8倍(图30)。
另外，图20总结了与野生型M124菌株相比，M188单一删除菌株、M219 双重删除菌株、M277三重删除菌株和M307四重删除菌株的针对酪蛋白的总 蛋白酶活性。与野生型M124菌株相比，pep1单一删除降低蛋白酶活性1.7 倍，pep1/tsp1双重删除降低蛋白酶活性2倍，pep1/tsp1/slp1三重删除降低蛋 白酶活性3.2倍，和pep1/tsp1/slp1/gap1四重删除降低蛋白酶活性7.8倍(图 20)。
MAB01抗体产生菌株M371包含四重删除Δpep1Δtsp1Δslp1Δgap1。菌株 在发酵罐中生长并与三重删除MAB01产生菌株在相同条件下进行比较。分 批培养用产生MAB01且具有pep1、tsp1、slp1和gap1蛋白酶删除和kex2过 表达的M371菌株进行。菌株在补充有40g/l废谷物提取物、40g/l葡萄糖和 40g/l乳糖的木霉属最低培养基，pH 5.5中生长。培养物在30℃下生长并随 后对于产生相切换到22℃。分批培养用产生MAB01且具有pep1、tsp1和slp1 蛋白酶删除和kex2过表达的M304菌株进行。菌株在补充有40g/l固体废谷 物、40g/l葡萄糖和40g/l乳糖的木霉属最低培养基，pH 5.5中生长。培养物 在30℃下生长并随后对于产生相切换到22℃。
从第6天样品，计算的全长抗体产率在gap1删除菌株中高20％。在相同 条件下，四重删除菌株产生1.9g/L(897mg/L全长抗体)和三重删除菌株产生 1.3g/L(731mg/L全长抗体)。从发酵罐上清液，测量针对酪蛋白的总蛋白酶 活性。上清液样品在柠檬酸钠缓冲液，pH 5.5中稀释以使得对于所有样品的 总蛋白浓度是0.15mg/ml。向这一稀释的上清液中添加10μg/ml BODIPY酪 蛋白以开始蛋白酶分析。来自各天的培养的样品在两个不同菌株之间比较。 结果显示，在第5天gap1删除菌株中具有最多低30％的总蛋白酶活性(图 31)。在第6天，蛋白酶活性低20％，其与该天抗体产率的20％提高相关。
五重删除菌株
产生具有五重删除Δpep1Δtsp1Δslp1Δgap1Δgap2的M369菌株并用于进 一步的蛋白酶删除。
五重蛋白酶删除菌株M369的产生
为产生无标记的五重蛋白酶菌株，pyr4 blaster盒的去除基本上如实施例 3中对于从单一蛋白酶删除菌株M195(Δpep1)去除pyr4 blaster盒所述应用于 菌株M307。完成三个相继的5-FOA选择步骤以确保所选择的克隆源自单一 细胞。采用标准实验室方法通过用表4.3中所列的引物的PCR验证blaster盒 的去除。通过与pyr4 blaster盒的去除部分退火的引物未看到特异性信号。去 除进一步通过在有或没有5mM尿苷的情况下将克隆接种到最低培养基板上 进行验证。在无尿苷补充的板上没有观察到生长。用于产生五重蛋白酶删除 菌株的克隆以菌株编号M321(Δpep1Δtsp1Δslp1Δgap1,pyr4-)命名。
用于第五蛋白酶基因(谷氨酸蛋白酶gap2(TreID106661))的删除质粒 pTTv145描述于实施例2中(表2.3)。这一删除质粒导致gap2基因座中的944 bp删除并覆盖GAP2的完整编码序列。
为移除载体序列，质粒pTTv145(Δgap2-pyr4环出)用PmeI消化并且正确 的片段使用QIAquick凝胶提取试剂盒(Qiagen)从琼脂糖凝胶纯化。大约5μg 的gap2删除盒用于转化以上M321(Δpep1Δtsp1Δslp1Δgap1,pyr4-)。原生质体 的制备和转化基本上如实施例1中对于菌株M181和M195所述的使用pyr4 选择进行。
100个克隆挑取作为第一划线。所有20条生长划线采用标准实验室方法 通过使用表4.3中所列的引物的PCR筛选正确整合。10个推定的五重蛋白酶 破坏子(Δpep1Δtsp1Δslp1Δgap1Δgap2)纯化为单细胞克隆并通过PCR重筛选。 仅一个纯化的克隆对于gap2ORF是阴性的。gap2删除通过克隆的DNA印迹 分析确认(图32A)。如实施例1中所述进行DNA印迹分析。DNA印迹分析 也表明克隆在基因组中其它地方携带另外的删除盒拷贝或具有该基因座中的 一些内部重排(图32B和32C)。由于这是所获得的唯一五重蛋白酶删除，它 被选择用于进一步应用(图32D和32E)。克隆14是用于去除pyr4 blaster盒的 克隆，且用于产生以下6重蛋白酶删除菌株M396和M400(图32E)。这一克 隆用菌株编号M369(Δpep1Δtsp1Δslp1Δgap1Δgap2)命名。
表4.3：用于筛选pyr4 blaster盒的去除和用于筛选gap2整合和菌株纯度的引物。
(50)用于筛选pyr4 blaster盒从M307的去除
用于筛选pTTv145的整合
用于筛选gap2 ORF的删除
五重蛋白酶删除菌株的分析
来自M369菌株的蛋白酶活性针对其亲本菌株M307测量。gap2蛋白酶 删除导致低23％的针对酪蛋白的蛋白酶活性(图33)。
6重删除菌株
产生具有删除Δpep1Δtsp1Δslp1Δgap1Δgap2Δpep4的6-重蛋白酶删除菌 株并用于进一步的蛋白酶删除。
pep4删除质粒的产生
用于第六蛋白酶基因(天冬氨酸蛋白酶pep4(TreID77579))的删除质粒 pTTv181基本上如实施例1中对于Δpep1质粒pTTv71所述构建。959bp的 5'侧翼区域和992bp的3'侧翼区域选择作为pep4删除质粒的基础。关于pep1， 用于pep4的第一删除质粒(pTTv43,表4.4)携带另一选择标记，bar，其用pyr4 blaster盒替代。blaster盒利用NotI消化从pTTv71获得，与NotI切割的pTTv43 连接，并随后使用标准方法转化到大肠杆菌中。培养一些转化体，分离并消 化质粒DNA以使用标准实验室方法筛选pyr4 blaster盒的正确连接和定向。具有正确插入大小和定向的一个克隆进行测序和储存(pTTv73,表4.4)。 blaster盒再一次轻微改变：用于去除pyr4的同向重复片段从308bp的pyr4 5'UTR改变为pep4 5'侧翼区域末端的300bp同向重复序列(如在pTTv145中, gap2-pyr4)。这通过利用NotI消化从pTTv73去除现有的pyr4 blaster盒而进 行。pyr4基因使用pTTv73作为模板利用表4.4中的引物通过PCR扩增。对 于克隆中使用的酵母同源重组系统，用于载体的重叠序列替代为合适的PCR引物。为能够实现构建体中的标记切换，NotI限制性位点引入pyr4选择标记 的两侧，且对于另外的克隆步骤，AscI位点引入pep4 5'同向重复和3'侧翼之 间。这一类型的blaster盒在切除后不应对删除基因的基因座遗留任何另外的 序列。300bp pep4 5'同向重复使用里氏木霉野生型菌株QM6a作为模板通过 PCR扩增。产物用琼脂糖凝胶电泳分离且正确的片段使用标准实验室方法用 凝胶提取试剂盒(Qiagen)从凝胶分离。培养从重组获得的几个克隆，且分离和 消化质粒DNA以使用标准方法筛选正确的重组。用于pep4的这些删除质粒(pTTv43,pTTv73和pTTv181,表4.4)导致pep4基因座中的1413bp删除并覆 盖PEP4的完整编码序列。
表4.4：用于产生pep4删除质粒的引物
用于pep4(TreID77579)的删除质粒pTTv43,载体骨架pRS426
用于pep4(TreID77579)的删除质粒pTTv73,载体骨架pTTv43
用于pep4(TreID77579)的删除质粒pTTv181,载体骨架pTTv73
6重蛋白酶删除菌株M396和M400的产生
为产生无标记的6重蛋白酶删除菌株，pyr4标记的去除基本上如实施例 3中对于从菌株M195(Δpep1)去除pyr4 blaster盒所述应用于菌株M369。完 成三个相继的5-FOA选择步骤以确保所选择的克隆源自单一细胞。最终克隆 采用标准实验室方法通过用表4.5中所列的引物的PCR验证。对于所有克隆 获得对应于blaster盒成功去除的信号。去除进一步通过在有或没有5mM尿 苷的情况下将克隆接种到最低培养基板上验证。在无尿苷补充的板上没有观 察到生长。六个推定pyr4-克隆的DNA印迹分析确认blaster盒从三个克隆去 除。另外，DNA印迹分析揭示这三个克隆丧失了对于亲本M369中gap2侧 翼观察到的额外信号。因此这些克隆应当具有gap2基因座中预期的基因组组 织。用于产生6重蛋白酶删除菌株的克隆以菌株编号M381 (Δpep1Δtsp1Δslp1Δgap1Δgap2,pyr4-)命名。
为移除载体序列，质粒pTTv181(Δpep4-pyr4环出)用PmeI消化并且正确 的片段使用QIAquick凝胶提取试剂盒(Qiagen)从琼脂糖凝胶纯化。大约5μg 的pep4删除盒用于转化M381(Δpep1Δtsp1Δslp1Δgap1Δgap2,pyr4-)。原生质 体的制备和转化基本上如实施例1中对于菌株M181和M195所述的使用pyr4 选择进行。
超过200转化体挑取作为第一划线。32条生长的划线通过PCR(使用表 4.5中所列的引物)筛选正确整合。七个克隆给出预期的信号并纯化为单细胞 克隆和使用表4.5中所列的引物通过PCR重筛选。pep4的删除采用实施例3 中对于M181和M195所描述的标准实验室方法通过五个克隆的DNA印迹分 析确认(图34A和34B)。DNA印迹分析也表明所有转化体(图34C和34D) 是单一整合体。为排除PCR筛选中对于转化体中pep4 ORF观察到的微弱信号源自pep4基因，三个克隆进一步经单一细胞步骤纯化和通过DNA印迹杂 交和PCR再分析。从表明菌株纯度的任一分析没有获得pep4 ORF的信号。 用于去除pyr4 blaster盒(且在产生七重蛋白酶删除菌株中)的克隆25-120A 用菌株编号M396命名且重纯化的克隆25-120A-a用菌株编号M400命名。
表4.5：用于筛选pyr4 blaster盒从M369的去除和用于筛选pep4整合和菌 株纯度的引物。
用于筛选pyr4 blaster盒从M369的去除
用于筛选pTTv181的整合
用于筛选pep4 ORF的删除
4-、5-和6-重蛋白酶删除菌株中蛋白酶活性的分析
四重蛋白酶删除菌株M307、五重蛋白酶删除菌株M369和6重蛋白酶删 除菌株转化体在摇瓶培养中培养。从用100mM PIPPS缓冲的含有20g/L废 谷物和40g/L乳糖的TrMM，pH4.8中生长的大摇瓶培养获取上清液样品。 pH在第5天是～4.25。测试的6重蛋白酶删除转化体不是最终菌株，因此存 在一些由于孢子纯度导致的变异。这些是最佳转化体中的一些，但随后进行 进一步的孢子纯化。第5天上清液在50mM柠檬酸钠缓冲液，pH 4.5中1:3 稀释。向该稀释的上清液中添加BODIPY酪蛋白FL(10μg/ml)并在37℃下 一起孵育4小时。蛋白酶活性分析如制造商的方案(enzCheck蛋白酶分析试 剂盒#E6638,Molecular Probes)中描述的进行。蛋白酶活性结果可以在图33 中看到。
当五重蛋白酶删除菌株M369在酸性条件下生长时，存在蛋白酶活性的 小的降低。菌株中gap2的删除提供针对酪蛋白的蛋白酶活性23％的降低。在 6重蛋白酶删除菌株中，天冬氨酸蛋白酶pep4在研究的5个转化体中删除。 最佳转化体与其亲本菌株M369相比显示35％的降低。
7重删除菌株的产生
产生具有删除Δpep1Δtsp1Δslp1Δgap1Δgap2Δpep4Δpep3的7重蛋白酶删除菌株并用于进一步的蛋白酶删除。
pep3删除质粒的产生
用于第七蛋白酶基因(天冬氨酸蛋白酶pep3(TreID121133))的第一删除 质粒pTTv188基本上如实施例1中对于Δpep1质粒pTTv41所述构建。1215bp 的5'侧翼区域和1082bp的3'侧翼区域选择作为pep3删除质粒的基础。关于 上述gap2(pTTv145)和pep4(pTTv181)删除质粒，在这一质粒中同向重复片 段是从pep3 5'侧翼区域的末端的300bp延伸。片段使用表4.6中所列的引物 通过PCR产生。对于上述pTTv181(Δpep4-pyr4)，为能够实现构建体中的标 记切换，NotI限制性位点引入pyr4选择标记的两侧，且对于另外的克隆步骤， AscI位点引入pep3 5'同向重复和3'侧翼之间。产物用琼脂糖凝胶电泳分离且 正确的片段使用标准实验室方法用凝胶提取试剂盒(Qiagen)从凝胶分离。侧翼 区域的PCR中使用的模板是里氏木霉野生型菌株QM6a。pyr4标记基因用 NotI消化从pTTv181获得。载体骨架是如实施例1中的EcoRI/XhoI消化的 pRS426。质粒使用实施例1中描述的酵母同源重组方法构建。
用于天冬氨酸蛋白酶pep3(TreID121133)的第二删除质粒pTTv192使用 如上的质粒pTTv188作为骨架构建。这一第二质粒携带原始KEX2(TreID 123156)过表达盒并使用来自构巢曲霉的乙酰胺酶(AmdS)基因作为选择标记。 pyr4 blaster盒利用NotI-AscI双重消化从pTTv188去除。对于cDNA1启动子(模 板：pTHN3质粒DNA)、原始kex2(模板：里氏木霉QM6a基因组DNA)、trpC 终止子(模板：pHHO2质粒DNA)和AmdS标记(模板：pHHO1质粒DNA)的片段使用表4.6中所列的引物通过PCR产生。对于上述pTTv188，为能够实 现构建体中的标记切换，NotI限制性位点引入AmdS选择标记的两侧。产物 用琼脂糖凝胶电泳分离且正确的片段使用标准实验室方法用凝胶提取试剂盒 (Qiagen)从凝胶分离。质粒使用实施例1中描述的酵母同源重组方法构建。
用于天冬氨酸蛋白酶pep3(TreID121133)的第三删除质粒pTTv205使用 如上的质粒pTTv192作为骨架构建。AmdS标记使用NotI消化从pTTv192去 除。用于新的pyr4 blaster盒的片段(位于KEX2过表达盒之后)使用表4.6中 所列的引物通过PCR产生。在这一blaster盒中，同向重复是从pep3 3'侧翼 区域开始的300bp延伸并位于pyr4基因之前。对于上述pTTv192，为能够实 现构建体中的标记切换，NotI限制性位点引入pyr4 blaster盒的两侧。产物用 琼脂糖凝胶电泳分离且正确的片段使用标准实验室方法用凝胶提取试剂盒(Qiagen)从凝胶分离。质粒使用实施例1中描述的酵母同源重组方法构建。
用于pep3的这些删除质粒(pTTv188、pTTv192和pTTv205,表4.6)导致 pep3基因座中的2590bp删除并覆盖PEP3的完整编码序列。
表4.6：用于产生pep3删除质粒的引物。
(51)用于pep3(TreID121133)的删除质粒pTTv188，载体骨架pRS426
用于pep3(TreID121133)的删除质粒pTTv192，载体骨架pTTv188
用于pep3(TreID121133)的删除质粒pTTv205，载体骨架pTTv192
7重蛋白酶删除菌株的产生
为产生无标记的7重蛋白酶删除菌株，pyr4标记的去除基本上如实施例 3中对于从菌株M195(Δpep1)去除pyr4 blaster盒所述应用于6重删除菌株 M396。完成四个相继的5-FOA选择步骤以确保所选择的克隆源自单一细胞。
最终克隆采用标准实验室方法采用表4.7中所列的引物通过PCR验证。 获得对应于blaster盒成功去除的信号。blaster盒的去除进一步通过在有或没 有5mM尿苷的情况下将克隆接种到最低培养基板上验证。在无尿苷补充的 板上没有观察到生长。四个推定pyr4-克隆的DNA印迹分析确认所有克隆的 blaster盒去除(图34E)。用于产生7重蛋白酶删除菌株的克隆(25-120A-62) 以菌株编号M402(Δpep1Δtsp1Δslp1Δgap1Δgap2Δpep4,pyr4-)命名。
完成两个平行转化；一个用pTTv188的删除构建体(标准pep3删除)和另 一个用pTTv205(包括KEX2过表达)。为移除载体序列，质粒pTTv188和 pTTv205用PmeI消化并且正确的片段使用QIAquick凝胶提取试剂盒(Qiagen) 从琼脂糖凝胶纯化。大约5μg的任一删除盒用于转化M402 (Δpep1Δtsp1Δslp1Δgap1Δgap2Δpep4,pyr4-)。原生质体的制备和转化基本上如 实施例1中对于菌株M181和M195所述的使用pyr4选择进行。
挑取转化体作为第一划线。生长的划线通过PCR(使用表4.7中所列的 引物)筛选正确整合。给出预期信号的克隆纯化为单细胞克隆并使用表4.7 中所列的引物通过PCR重筛选。
pep3的删除采用实施例1中描述的方法通过所选择克隆的DNA印迹分 析确认。选择的克隆用于去除pyr4 blaster盒且用于8重蛋白酶删除菌株的产 生(图34E)。
表4.7：用于筛选pyr4 blaster盒从M396的去除和用于筛选pep3整合和菌株 纯度的引物。
(52)用于筛选pyr4 blaster盒从M396的去除
用于筛选pTTv188的整合
用于筛选pTTv205的整合
(53)用于筛选pep3 ORF的删除
8重删除菌株的产生
产生了具有删除Δpep1Δtsp1Δslp1Δgap1Δgap2Δpep4Δpep3Δpep5的8重蛋白酶删除菌株。
pep5删除质粒的产生
用于第八蛋白酶基因(天冬氨酸蛋白酶pep5(TreID81004))的第一删除 质粒基本上如实施例1中对于Δpep1质粒pTTv41所述构建，但另外的第二 选择标记盒(bar,实施例1)放置在pyr4基因之后从而产生具有双重选择标记 blaster盒的删除质粒。双重标记系统使得能够a)利用例如bar(或hph，用于 潮霉素抗性的盒)作为初始抗性标记和较快的选择；b)pyr4+菌株的转化(在转 化前不需要产生pyr4-)；和c)使用5-氟乳清酸从转化体去除两个标记(像标准 pyr4 blaster盒的去除中一样)和产生无标记的pyr4-菌株的内源pyr4的同时诱 变。
用于天冬氨酸蛋白酶pep5(TreID81004)的第二删除质粒pTTv229使用如 上的质粒pTTv202作为骨架构建。pyr4-bar双标记利用NotI消化从pTTv202 去除。pyr4标记基因利用NotI消化从pTTv181获得。质粒pTTv229的克隆 使用T4DNA连接酶在室温下通过标准连接反应进行。连接混合物的部分利 用电穿孔转化到大肠杆菌中。培养几个克隆，分离和消化质粒DNA以使用 标准实验室方法筛选正确连接。标记的正确连接和定向进一步通过测序验证。 用于pep5的这些删除质粒(pTTv202和pTTv229，表4.8)导致pep5基因座中 的1687bp删除并覆盖PEP5的完整编码序列。
1348bp的5'侧翼区域和1164bp的3'侧翼区域选择作为pep5删除质粒的 基础。从pep5 5'侧翼末端的300bp延伸用作同向重复片段。这些片段以及第 二选择标记盒bar(实施例1)利用表4.8中所列的引物通过PCR扩增。产物用 琼脂糖凝胶电泳分离且正确的片段使用标准实验室方法用凝胶提取试剂盒 (Qiagen)从凝胶分离。为能够实现完整双重标记盒的去除，NotI限制性位点引 入双重标记盒的两侧，且AsiSI位点引入两个选择标记之间。AscI位点引入 pep5 5'同向重复和3'侧翼之间。载体骨架是实施例1中EcoRI/XhoI消化的pRS426。pyr4选择标记利用NotI消化从pTTv181(以上Δpep4-pyr)获得。质 粒使用实施例1中描述的酵母同源重组方法构建。用于pep5的这一删除质粒 (pTTv202，表4.8)导致pep5基因座中的1687bp删除并覆盖PEP5的完整编 码序列。
表4.8：用于产生pep5删除质粒的引物
(54)用于pep5(TreID81004)的删除质粒pTTv202，载体骨架pRS426
用于pep5(TreID81004)的删除质粒pTTv229，载体骨架pTTv202
8重蛋白酶删除菌株的产生
为产生无标记的8重蛋白酶删除菌株，pyr4标记的去除基本上如实施例 3中对于从菌株M195(Δpep1)去除pyr4 blaster盒所述应用于7重删除菌株 M486(34-14A-a,M402中的pTTv205)。完成四个相继的5-FOA选择步骤以确 保所选择的克隆源自单一细胞。
最终克隆采用标准实验室方法通过用表4.9中所列的引物的PCR验证。 对于大多数克隆获得对应于blaster盒的成功去除的信号。blaster盒的去除进 一步通过在有或没有5mM尿苷的情况下将克隆接种到最低培养基板上验 证。在无尿苷补充的板上没有观察到生长。推定的pyr4-克隆的DNA印迹分 析确认blaster盒的去除。
为移除载体序列，质粒pTTv229用PmeI+XbaI消化并且正确的片段使 用QIAquick凝胶提取试剂盒(Qiagen)从琼脂糖凝胶纯化。大约5μg的删除盒 用于转化8重蛋白酶删除菌株(Δpep1Δtsp1Δslp1Δgap1Δgap2Δpep4Δpep3, pyr4-)的克隆。原生质体的制备和转化基本上如实施例1中对于菌株M181和 M195所述的使用pyr4选择进行。
转化体挑取作为第一划线。生长的划线通过PCR(使用表4.9中所列的 引物)筛选正确整合。给出预期的信号的克隆纯化为单细胞克隆并使用表4.9 中所列的引物通过PCR重筛选。pep5的删除采用实施例1中所描述的方法通 过选择的克隆的DNA印迹分析确认。
表4.9：用于筛选pyr4 blaster盒从7重菌株的去除和用于筛选pep5整合和 菌株纯度的引物
(55)用于筛选pyr4 blaster盒从M486的去除和菌株纯度
用于筛选pTTv229的整合
用于筛选pep5 ORF的删除
实施例5—利用蛋白酶抑制剂提高抗体产生
本实施例证明蛋白酶抑制剂提高里氏木霉产生菌株中全长抗体产生的能 力。
基于重链被胰蛋白酶和胰凝乳蛋白酶切割的知识，这两类蛋白酶的抑制 剂在体外和培养实验中利用产生抗体的里氏木霉菌株针对抗体降解进行测 试。测试了抑制剂大豆胰蛋白酶抑制剂(SBTI)和胰凝乳蛋白酶抑制剂，因为 它们先前在体外试验中显示为稳定抗体重链。
体外抑制剂处理
胰凝乳蛋白酶抑制剂和SBTI在体外用培养上清液进行分析。来自分批 补料发酵培养的上清液用柠檬酸钠缓冲液pH 5.5(pH 5.5；28℃；20g/L废谷物 提取物,60g/L乳糖)稀释到6mg/ml。分批补料培养用里氏木霉野生型菌株 M44进行，其不包含异源蛋白质表达。菌株在28℃下补充有20g/l废谷物提 取物和60g/l乳糖的木霉属最低培养基，pH 5.5中生长。
向这一稀释的上清液中添加0.05μg/μl的利妥昔单抗、100uM胰凝乳蛋 白酶抑制剂、1mg/ml SBTI或两种抑制剂的组合到50μl的总体积，并在0、 1和19小时取样以评估利妥昔单抗抗体重链的早期和晚期降解。所得的重链 产物使用在TBST中1:30,000稀释的抗重链AP偶联抗体(Sigma#A3188)通过 免疫印迹进行分析(图35)。从重链产生的初始降解产物为大约42kDa和38 kDa，其在1小时时在未处理的对照道中观察到(图35)。另外的片段在19小 时后产生，两种主要产物得到保留。胰凝乳蛋白酶抑制剂处理抑制42kDa片 段的初始产生，而SBTI处理抑制38kD片段的形成(图35)。组合两种化合物 抑制约96％的初始重链降解和19小时后约75％的降解(图35)。这些结果证明 两种抑制剂能够有效地稳定利妥昔单抗抗体重链。
用抑制剂处理里氏木霉培养
抑制剂的有效性也利用利妥昔单抗抗体产生菌株测试，其包含VALEKR 连接体序列和pep1删除。这一菌株在胰凝乳蛋白酶抑制剂、SBTI或胃蛋白 酶抑制剂A存在或不存在的情况下在小烧瓶中一式三份地生长。小的摇瓶包 含50ml的TrMM加乳糖(40g/l)、废谷物提取物(20g/l)并用100mM PIPPS 缓冲，pH 5.5。SBTI抑制剂以100μg/ml或500μg/ml的最终浓度添加到培养 中。胰凝乳蛋白酶抑制剂以100μM使用，和胃蛋白酶抑制剂A以10μM使 用。三种抑制剂各自在第2、3、4和5天每天添加。
培养物的生长在第2天到第7天每天跟踪pH。对于用SBTI生长的培养， 与未处理的对照相比，培养物的pH没有显著差异。在PIPPS缓冲的培养物 中，pH从初始的pH 5.5降低到第6天后的pH 4.8。利用胰凝乳蛋白酶抑制 剂和胃蛋白酶抑制剂A，培养物监测直到第7天。在第7天，对照瓶的平均 pH是4.6，而胰凝乳蛋白酶抑制剂处理的培养物的平均pH是4.9，和胃蛋白 酶抑制剂A处理的培养物的平均pH是5.0。因此，对于用胰凝乳蛋白酶抑制 剂和胃蛋白酶抑制剂A处理的培养物，存在生长的小降低。
培养上清液样品(30μl)也在第3、4和5天收集用于抗体产生的分析。分 析用抗IgG重链抗体AP偶联物(Sigma#A3188)和抗轻链抗体AP偶联物 (Sigma#A3812)通过免疫印迹进行。各抗体在TBST中1:30,000稀释。全长 利妥昔单抗重链为约51kD，利妥昔单抗轻链CHBI融合体为约100kD，和 游离利妥昔单抗轻链为约28kD。
用SBTI处理的培养上清液样品的免疫印迹分析的结果显示于图36中。 在取样的所有天，在SBTI处理的培养物中比未处理的培养物存在更多的全 长利妥昔单抗重链和更多的38kD降解产物。在第5天，利妥昔单抗重链产 量在SBTI处理的培养物中比未处理的培养物高几倍(图36A)。因此，SBTI 的使用对提高利妥昔单抗重链产量具有积极效果。在整体利妥昔单抗轻链产 生中也具有轻微改善，特别是对于载体结合的轻链(图36B)。
用胰凝乳蛋白酶抑制剂和胃蛋白酶抑制剂A处理的培养上清液样品的免 疫印迹分析的结果显示于图37中。胰凝乳蛋白酶抑制剂处理的培养物显示出 与用SBTI处理的培养物观察到的相似的结果(图37)。利妥昔单抗重链在第5 天稳定(图37B)。当与未处理的对照培养物相比，胰凝乳蛋白酶抑制剂提高产 生的全长利妥昔单抗重链的量，虽然仍观察到38kD的主要降解产物。
总体上，它表现为SBTI处理在促进更高的蛋白质产生方面比胰凝乳蛋 白酶抑制剂处理更有效。但是，胰凝乳蛋白酶抑制剂处理产生更高的全长利 妥昔单抗重链与片段比率。如图37B中看到的，第三胰凝乳蛋白酶抑制剂培 养样品显示与10％重链片段相比的大约90％的全长利妥昔单抗重链。因此， SBTI和胰凝乳蛋白酶抑制剂处理的组合对于获得更高的抗体产率是非常有 益的。
实施例6-里氏木霉抗体产生
本实施例定量以上实施例1-4中描述的里氏木霉蛋白酶删除菌株中产生 的抗体量。
抗体纯化
来自表6.1所列的各里氏木霉蛋白酶删除菌株的培养物上清液通过0.45 μm注射器式滤器过滤并在纯化前通过添加1/50体积的1M磷酸钠，pH 7调 节到结合缓冲液的组成。亲和柱与Purifier连接，且纯化按照制造商的 指示进行。使用以下色谱条件：流速，1ml/min；检测280nm；注射环，5ml； 缓冲液A，20mM磷酸钠，pH 7；缓冲液B，0.1M甘氨酸-HC1，pH 2.7。进 行缓冲液A的等度运行直到开始洗脱，其用5ml的缓冲液B进行。柱在各 次分析前用至少5ml的缓冲液A平衡。1ml的培养物上清液注射用于定量运 行。在洗脱过程中，0.5ml级分收集到包含40μ1的0.5M Tris，pH 9的管中。 抗体在两个级分中剧烈地洗脱，其汇集到1ml的一个样品中。从各样品系列 (发酵)中具有最高峰面积的样品，进行具有5-10ml注射量的一次运行以 获得更浓缩的样品用于凝胶过滤分析。
对于定量，进行抗体标准的一系列稀释并在与所分析的样品类似地在 HiTrapProtein G柱中以1ml运行。建立280nm下测量的峰面积的标准曲线 (10-500μg)并用于从培养上清液分离的MAB01或利妥昔单抗抗体的定量。纯 化的样品的质量通过SDS-PAGE检验。
蛋白G纯化的样品的凝胶过滤特征
各纯化的MAB01和利妥昔单抗抗体的250μ1样品在与Purifier HPLC系统连接的Superdex 200 10/300GL凝胶过滤柱(Amersham Biosciences) 中Tris缓冲盐水(25mM Tris,140mM NaCl和3mM KC1,pH7.4)中运行。流 速为0.75ml/min且吸光度在280nm下测量。级分(0.75ml)在整个运行期间收 集。仅显示一个峰的级分进行浓缩并在标准SDS-PAGE凝胶上表征(图38)。 洗脱的各峰的百分比通过用280nm下测量的样品的总面积除该峰面积计算。
抗体纯化过程显示在图38中。
抗体定量
里氏木霉蛋白酶删除菌株产生的抗体量总结于表6.1中。
表6.1：由实施例中描述的里氏木霉菌株的粗发酵上清培养产生的抗体量和 质量的总结。
在表6.1中，抗体(mAb)的总量是从培养上清液纯化的蛋白质的量。在蛋 白质纯化后，抗体在尺寸排阻色谱中运行以测量全长组装抗体的量。该量之 后称为“完全mAb”。
如表6.1中所示，M304三重删除菌株(Δpep1Δtsp1Δslp1)实现3500mg/L 总IgG的抗体产率，其中2500mg/L正确组装成全长MAB 01抗体。这对应 于71％的全长抗体。全长抗体百分比的提高是slp1删除的结果。与M304菌 株相反，M247双重删除菌株(Δpep1Δtsp1)实现全长抗体的43％的产率(pH 5.5； 22℃；9g/l酪蛋白氨基酸；20g/L废谷物提取物,60g/L乳糖)。因此，可以直 接看到Δslp1删除的增加显著提高产物质量(提高25％)。
M507 MAB01产生菌株中抗体质量的提高
产生了两种MAB01产生菌株，3蛋白酶删除背景中的M304和7蛋白酶 删除背景中的M507。构建了具有单独的用于重链和轻链的盒的M304菌株(图 49)。重链整合到cbh1基因座中和轻链整合到egl1基因座中。M507菌株通 过将包含重链和轻链两者的串联盒整合到CBHI基因座中形成(图49)。 MAB01串联载体沿相反方向确定重链和轻链的方向。轻链使用NVISKR切 割位点和重链使用DGETVVKR切割位点。M304菌株删除了3种蛋白酶pep1、 tsp1和slp1。M507删除了7种蛋白酶pep1、tsp1、slp1、gap1、gap2、pep4、 pep3。两个菌株都过表达kex2蛋白酶。
MAB01双向串联载体pTTv223使用标准原生质体转化转化到具有kex2 过表达的七重蛋白酶删除菌株M486中。转化体在乙酰胺-氚板上选择且第一 划线对于AmdS标记5'和3'整合到cbh1基因座进行PCR筛选。双重阳性转 化体通过单孢子培养纯化且孢子原种(sporestock)在补充氨苄青霉素的PD板 上产生。
M304和M507菌株在28℃下具有30g/l葡萄糖、60g/l废谷物、60g/l 乳糖及乳糖补料的发酵罐中培养并随后在培养中切换到22℃。M507菌株在 pH 5.2(培养bio00541b)和pH5.5(培养bio00543b)下培养。M304菌株在pH 5.5 (培养bio00503b)下生长。M304发酵bio00477b样品在bio00503b免疫印迹 中包括作为对照。bio00477b培养用对于bio00503b描述的相同培养基和条件 进行。
M507菌株在pH 5.2和pH 5.5下培养以研究pH对抗体产生的影响。来 自pH 5.2发酵的上清液样品通过蛋白质印迹分析并显示在图50A中，和来自 pH 5.5发酵的样品显示在图50B中。产生的抗体在两个M507培养中看起来 相当相似。在pH 5.2条件下具有稍微更多的轻链。在两个培养中具有重链片 段。M304菌株在pH 5.5下培养，和结果可以在图51中看到。产生的全长重 链的量在M304菌株中第7天后下降。重链的量在M507菌株中第9天后下降。菌株之间的其它差异是产生的轻链的量。M304产生得显著更多。
来自三个发酵运行的蛋白G纯化的免疫球蛋白浓度可以在表6.2中看到。 对于M304菌株的最高总抗体浓度在第9天是3.1g/l。对于M507菌株的最高 浓度第10天在pH 5.2下是3.0g/l和在pH 5.5下是2.8g/l。在尺寸排阻色谱 后，全长抗体的量对于各样品进行计算(表6.3)。最高全尺寸抗体量对于M507 发酵在第8天(pH 5.2)和第9天(pH 5.5)为2.0g/l。M304在第8天产生类似的 2.0g/l水平的全长抗体。
表6.2：在培养样品的蛋白G纯化后测定的总抗体浓度
总Ab M304 M507 M507
bio00503b bio00541b bio00543b
pH 5.5 pH 5.2 pH 5.5
天 mg/L mg/L mg/L
5 2300 1200 1164
6 2800 1427 1454
7 3000 2211 2116
8 3000 2745 2455
9 3100 2721 2709
10 2100 2995 2808
11 2702 2164
M304和M507菌株之间的差异在考虑随培养的时间过程产生的全长抗 体的百分比时变得显而易见。与菌株M304相比，M507的全长抗体的百分 比较高。在pH 5.5下生长的M507菌株产生最高质量的抗体，在第7天最高 78％为全长抗体。M304第6天达到68％，但随后产物质量与M507相比降低。 M507产物直到第9天是73％全长抗体。
表6.3：全长抗体的浓度在尺寸排阻色谱后计算
全尺寸Ab M304 M507 M507
bio00503b bio00541b bio00543b
pH 5.5 pH 5.2 pH 5.5
天 mg/L mg/L mg/L
6 1900 1062 1074
7 2000 1557 1645
8 2000 1998 1859
9 1700 1924 1988
10 900 1871 1806
表6.4：随培养的时间过程产生的全长抗体的百分比
全尺寸％ M304 M507 M507
bio00503b bio00541b bio00543b
pH 5.5 pH 5.2 pH 5.5
天 ％ ％ ％
6 68 74 74
7 67 70 78
8 60 73 76
9 56 71 73
10 44 62 64
蛋白酶活性测量
上清液中的蛋白酶活性在相同条件下生长的M304和M507菌株之间进 行比较。Triab62和Triab67培养在28℃下在30g/l葡萄糖、60g/l乳糖、20g/l 全废谷物、20g/l废谷物提取物及乳糖补料中pH 5.5下生长并随后在培养中 切换到22℃。
蛋白质浓度从第2-7天的所有上清液样品测定。所有上清液在柠檬酸钠 缓冲液pH5.5中稀释以使得所有样品具有0.625mg/ml的总蛋白质浓度。100 μl的所有稀释的上清液添加到黑色96孔板，每样品使用3个重复孔。在柠檬 酸钠缓冲液pH 5.5中制备的100μl的酪蛋白FL稀释原液(10μg/ml)添加到各 含上清液的孔中。板在37℃下覆盖在塑料包中孵育。来自孔的荧光在2、3 和4小时后测量。读数使用485nm激发和530nm发射在Varioskan荧光读板 仪上进行。
7重蛋白酶删除菌株M507的上清液中的蛋白酶活性比M304(3蛋白酶删 除)低2-2.5倍，参见表6.5。删除的酸性蛋白酶(gap1,gap2,pep4,pep3)造成这 种改进。7重删除菌株的一般蛋白酶活性对于酪蛋白底物显著降低。这一数 据总体上与对于全长抗体百分比观察到的结果相关。较低的蛋白酶活性导致 较高质量的抗体。
表6.5：pH 5.5培养物的发酵上清液中的蛋白酶活性。酪蛋白用作测量的底 物。
M304 M507
Triab62 Triab67
天 pH 5.5 pH 5.5
1 1.4 1.3
2 8.5 1.8
3 67.4 4.1
4 98.8 38.8
5 102.3 48.3
6 83.3 54.6
7 103.1 54.5
8 109.8 55.3
9 120.9 61.1
10 134.9 70.0
11 148.0 76.2
实施例7—非抗体蛋白质的产生
模型蛋白质IGF1、hGH和IFNα2b的蛋白酶稳定性通过将它们掺入6重 蛋白酶删除菌株(Δpep1Δtsp1Δslp1Δgap1Δgap2Δpep4)M400的上清液中进行 分析。上清液从大的摇瓶培养CAH15收集。来自摇瓶培养CAH15的未稀释 上清液在有或没有胃蛋白酶抑制剂A(50μM)和SBTI(0.2mg/ml)抑制剂的情 况下与纯化的模型蛋白质在37℃下孵育20小时。5天培养上清液pH是约 4.2。含0.05μg/μl的模型蛋白质的反应在20小时后取样。掺有模型蛋白质(0.05 μg/μ1)的50mM柠檬酸钠pH 4.0用作缓冲液对照。
从各反应，10μl加载到18％SDS PAGE凝胶上并在200V下运行30分 钟。凝胶中的蛋白酶然后转移到硝基纤维素用于免疫印迹。硝基纤维素膜在 室温下用TBST缓冲液中的5％乳封闭1小时。单个印迹在振摇器上室温下 用其特异性初级抗体探测1小时以检测适当的模型蛋白质。小鼠抗IGFl抗体 (R&D systems#mab291)以TBST中稀释的2μg/ml使用。小鼠抗rhGH抗体 (Abcam#ab51232)以TBST中稀释的2μg/ml使用。小鼠抗IFNα2b抗体(Abcam #ab9386)以TBST中稀释的1μg/ml使用。在用TBST简短洗涤印迹膜后，二 级抗体在室温振摇条件下添加1小时。次级山羊抗小鼠AP偶联抗体(Biorad #170-6520)在TBST中1:10,000稀释。
当在上清液中孵育过夜时，对于hGH、IFNα2b和IGF1观察到全长蛋白 质，虽然大多数表现为降解(图42)。对于人生长激素和IFNα2b存在约15kD 的主要降解产物。但是，这3种模型蛋白质在用天冬氨酸蛋白酶抑制剂胃蛋 白酶抑制剂A处理上清液后显著稳定。这种抑制剂阻断负责大多数蛋白酶活 性的关键蛋白酶。SBTI仅提供对于产物稳定性的小利益。虽然对于SBTI最 适宜的pH高于试验中使用的(pH 4.2vs.最佳pH 8.0)且因此这些抑制剂与其 靶蛋白酶的结合可能不是最有效的。
胃蛋白酶抑制剂A有效地抑制天冬氨酸蛋白酶。从胃蛋白酶抑制剂A的 亲和纯化研究已知，上清液中的其余天冬氨酸蛋白酶是pep2、pep3和pep5。 因此，如果删除其余2或3种天冬氨酸蛋白酶，则上清液几乎没有天冬氨酸 蛋白酶活性。对于这些模型蛋白质的产生，天冬氨酸蛋白酶pep2、pep3和 pep5被认为是主要蛋白酶。
这种相同的掺入试验如对于MAB01一样进行以研究其在具有或没有抑 制剂的情况下6蛋白酶删除上清液中的稳定性(图43)。如上所述获取样品并 用抗重链AP偶联抗体(Sigma#A3188)进行免疫印迹。在20小时孵育后，没 有重大重链降解。使用抑制剂没有明显益处。抗体在这种pH 4.2上清液中稳 定。在更酸性条件如pH 4.5下MAB01的产生很可能提高产率或至少降低将 产生的重链切割的量。
为评估哪些抑制剂最好地稳定hGH的产生，进行这些菌株的24孔培养。 M369人生长激素菌株(Δpep1Δtsp1Δslp1Δgap1Δgap2)与以下单一组分或其组 合一起生长：胰蛋白酶和枯草溶菌素抑制剂SBTI、酸性蛋白酶抑制肽SIP、 酸性蛋白酶抑制肽LIP、天冬氨酸蛋白酶抑制剂胃蛋白酶抑制剂A游离肽、 固定于琼脂糖珠的胃蛋白酶抑制剂A、来自利马豆的胰蛋白酶和枯草溶菌素 抑制剂BBI、枯草溶菌素抑制剂胰凝乳蛋白酶抑制剂及BSA。三个独立的孔 选择用于对照孔，其中未添加抑制剂或补充剂。这两个菌株在具有柠檬酸二铵(而没有硫酸铵)、100mM PIPPS、20g/L废谷物提取物、40g/L乳糖和调 节到pH 4.5的3mlTrMM中生长。24孔板在800rpm振摇、85％湿度和28℃ 下。培养物生长6天并用空气透过性膜覆盖。
抑制剂首次在第1天添加并随后在第3天开始每日添加。100μl样品在 第3天开始从培养孔获取。菌丝体以13k旋转5分钟并收集上清液。从培养 上清液，12μl加载到4-20％SDS PAGE凝胶中并用TBST中1:10,000稀释的 小鼠抗hGH抗体(2μg/ml)和山羊抗小鼠IgGAP偶联二级抗体在硝基纤维素 上进行免疫印迹。
在第4天，人生长激素仍可能在所有3个对照道的培养上清液中看到(图 44)。对照道中的两个显示弱条带和一个对照道显示轻微条带。抑制剂和补充 剂的效果立即观察到。具有大效应的抑制剂/补充剂以红色突出显示和具有最 佳效应的抑制剂/补充剂加星号。胃蛋白酶抑制剂A对生长激素产生具有负效 应。当以5或20μM使用时，hGH的产生似乎是不存在的。它表现为对产生 具有一些毒性效应。仅当胃蛋白酶抑制剂固定到琼脂糖珠上时，该效应取消。 最佳处理之一是胃蛋白酶抑制剂A珠加0.2mg/ml SBTI(参见图44中印迹上的第三星号)。对于仅SBTI(0.2mg/ml)，具有提高的产量，但具有存在于18kD 处的大降解条带，这表现为通过天冬氨酸蛋白酶的作用产生。如所预期的， 这一降解产物通过添加胃蛋白酶抑制剂A珠或SIP肽抑制剂减少。SIP肽甚 至在以20或50μM单独使用时是有益的。当使用SIP肽时，hGH量具有显 著提高，但当与SBTI或BSA组合使用时发生最大提高。当使用5uM和25uM 胰凝乳蛋白酶抑制剂时，也观察到全长产物量的提高。培养用单独的BSA (0.25％)补充有利于产生，但不阻止大的降解产物形成。
相对于200ng对照样品来估计表达水平，对照孔产生3-6mg/L的hGH， BSA(0.25％)/SIP(50μM)/SBTI(0.2mg/ml)处理产生24.5mg/L，SIP(50 μM)/SBTI(0.2mg/ml)处理产生26.6mg/L，和胃蛋白酶抑制剂A珠/SBTI(0.2 mg/ml)添加产生24.5mg/L的hGH。因此，使用抑制剂和添加剂的组合最好 地发现作用，提高产生水平至少4倍。关键因素是在基质中包括天冬氨酸蛋 白酶抑制剂。
实施例8—PEP7缺陷里氏木霉的产生
用于天冬氨酸蛋白酶pep7(TreID58669)的删除质粒基本上如实施例1中 对于pep1删除质粒pTTv41所述构建。1062bp的5'侧翼区域和1121bp的3' 侧翼区域选择作为pep7删除质粒的基础。片段使用表8.1中所列的引物通过 PCR产生。产物用琼脂糖凝胶电泳分离且正确的片段使用标准实验室方法利 用凝胶提取试剂盒(Qiagen)从凝胶分离。用于侧翼区域的PCR中的模板来自 里氏木霉野生型菌株QM6a。pyr4 blaster盒用NotI消化从pTTv71(实施例1) 获得。载体骨架是如实施例1中的EcoRI/XhoI消化的pRS426。质粒使用实施例1中描述的酵母同源重组方法构建。用于pep7的这一删除质粒导致pep7 基因座中的删除并覆盖PEP7的完整编码序列。
表8.1：用于产生pep7删除质粒的引物
(56)用于pep7(TreID58669)的删除质粒,载体骨架pRS426
用于筛选pep7删除盒的整合
用于筛选pep7 ORF的删除
实施例9—SLP5缺陷里氏木霉的产生
用于天冬氨酸蛋白酶slp5(TreID64719)的删除质粒基本上如实施例1中 对于pep1删除质粒pTTv41所述构建。1044bp的5'侧翼区域和1003bp的3' 侧翼区域选择作为slp5删除质粒的基础。片段使用表9.1中所列的引物通过 PCR产生。产物用琼脂糖凝胶电泳分离且正确的片段使用标准实验室方法利 用凝胶提取试剂盒(Qiagen)从凝胶分离。用于侧翼区域的PCR中的模板来自 里氏木霉野生型菌株QM6a。pyr4 blaster盒用NotI消化从pTTv71(实施例1) 获得。载体骨架是如实施例1中的EcoRI/XhoI消化的pRS426。质粒使用实施例1中描述的酵母同源重组方法构建。这一用于slp5的删除质粒导致slp5 基因座中的删除并覆盖SLP5的完整编码序列。
表9.1：用于产生slp5删除质粒的引物
(57)用于slp5(TreID64719)的删除质粒,载体骨架pRS426
用于筛选slp5删除盒的整合
用于筛选slp5 ORF的删除
实施例10-SLP6缺陷里氏木霉的产生
用于天冬氨酸蛋白酶slp6(TreID121495)的删除质粒基本上如实施例1中 对于pep1删除质粒pTTv41所述构建。1192bp的5'侧翼区域和1114bp的3' 侧翼区域选择作为slp6删除质粒的基础。片段使用表10.1中所列的引物通过 PCR产生。产物用琼脂糖凝胶电泳分离且正确的片段使用标准实验室方法利 用凝胶提取试剂盒(Qiagen)从凝胶分离。用于侧翼区域的PCR中的模板来自 里氏木霉野生型菌株QM6a。pyr4 blaster盒用NotI消化从pTTv71(实施例1) 获得。载体骨架是如实施例1中的EcoRI/XhoI消化的pRS426。质粒使用实施例1中描述的酵母同源重组方法构建。这一用于slp6的删除质粒导致slp6 基因座中的删除并覆盖SLP6的完整编码序列。
表10.1：用于产生slp6删除质粒的引物
(58)用于slp6(TreID121495)的删除质粒,载体骨架pRS426
用于筛选slp6删除盒的整合
用于筛选slp6 ORF的删除
实施例11—SLP7缺陷里氏木霉的产生
slp7删除质粒的产生
用于丝氨酸蛋白酶slp7(tre123865)的删除质粒pTTv269基本上如实施例 1中对于pep1删除质粒pTTv41所述构建，除了用于选择的标记是来自 pTTv194的pyr4-hgh。
949bp的5'侧翼区域和1025bp的3'侧翼区域选择作为slp7删除质粒 pTTv269的基础。这些片段使用表11.1中所列的引物通过PCR产生。用于侧 翼区域的PCR中的模板来自里氏木霉野生型菌株QM6a。产物用琼脂糖凝胶 电泳分离且正确的片段使用标准实验室方法利用凝胶提取试剂盒(Qiagen)从 凝胶分离。pyr4-hgh盒用NotI消化从pTTv194(Δpep4-pyr4-hgh)获得。为能 够实现标记盒的去除，NotI限制性位点引入盒的两侧。载体骨架是如实施例 1中的EcoRI/XhoI消化的pRS426。质粒pTTv269使用实施例1中描述的酵 母同源重组方法利用5'侧翼、3'侧翼、pyr4-hgh标记和载体骨架构建。这一用 于slp7的删除质粒(pTTv269，表11.1)导致slp7基因座中的2019bp删除并覆 盖SLP7的完整编码序列。
表11.1：用于产生slp7删除质粒的引物
(59)删除质粒pTTv269(Δslp7-pyr4-hgh)，载体骨架pRS426
实施例12-SLP8缺陷里氏木霉的产生
slp8删除质粒的产生
用于枯草溶菌素样蛋白酶slp8(tre58698)的删除质粒pTTv330基本上如 实施例1中对于pep1删除质粒pTTv41所述构建，除了用于选择的标记是双 重标记pyr4-hph。
975bp的5'侧翼区域和1038bp的3'侧翼区域选择作为slp8删除质粒的 基础。从slp8 5'侧翼末端的298bp延伸用作同向重复片段。这些片段使用表 12.1中所列的引物通过PCR扩增。产物用琼脂糖凝胶电泳分离且正确的片段 使用标准实验室方法利用凝胶提取试剂盒(Qiagen)从凝胶分离。pyr4-hph盒用 NotI消化从pTTv210(Δsepl-pyr4-hph)获得。为能够实现完整双重标记盒的去 除，NotI限制性位点引入双重标记盒的两侧。AscI位点引入slp8 5'同向重复 和3'侧翼之间。载体骨架是如实施例1中的EcoRI/XhoI消化的pRS426。质 粒使用实施例1中描述的酵母同源重组方法构建。这一用于slp8的删除质粒(pTTv330，表12.1)导致slp8基因座中的1433bp删除并覆盖SLP8的完整编 码序列。
表12.1：用于产生slp8删除质粒的引物
(60)删除质粒pTTv330(Δslp8-pyr4-hph),载体骨架pRS426
(61)
实施例13-蛋白酶同源物
里氏木霉pep1、pep2、pep3、pep4、pep5和pep7；tsp1；slp1、slp2、slp3、 slp5、slp6、slp7和slp8；gap1和gap2；及tpp1同源物从其它生物体鉴别。
BLAST检索使用里氏木霉蛋白酶氨基酸序列作为查询序列利用National Centerfor Biotechnology Information(NCBI)非冗余氨基酸数据库进行。或者， FASTA检索利用European Bioinformatics Institute(EBI)的UniProt Knowledgebase进行。绿色木霉和深绿木霉BLAST检索利用DOE Joint Genome Institute的网站(分别绿色木霉Gv29-8 v2.0和深绿木霉v2.0)进行。 BLAST检索命中的序列使用EBI提供的ClustalW2比对工具进行比对。系统 发生树也使用序列比对产生。
图45描绘所选择丝状真菌的天冬氨酸蛋白酶的系统发生树。
图46描绘所选择丝状真菌的枯草溶菌素蛋白酶的系统发生树。
图47描绘所选择丝状真菌的谷氨酸蛋白酶的系统发生树。
图48描绘所选择丝状真菌的Sedolisin蛋白酶的系统发生树。
实施例14-9重蛋白酶删除菌株的产生
具有删除Δpep1Δtsp1Δslp1Δgap1Δgap2Δpep4Δpep3Δpep5Δpep2的9重蛋 白酶删除菌株的产生
新的pep2删除质粒的产生
用于天冬氨酸蛋白酶pep2(tre0053961)的第一删除质粒pTTv213基本上 如实施例1中对于Δpep1质粒pTTv41所述构建，但携带潮霉素磷酸转移酶 基因(hph)的附加的第二选择标记置于pyr4基因之后产生具有双重选择标记blaster盒的删除质粒。双重标记系统使得能够a)利用例如hph作为初始抗性 标记并较快地选择；b)转化pyr4+菌株(在转化前不需要产生pyr4-)；和c)使 用5-氟乳清酸从转化体去除两个标记(像标准pyr4 blaster盒的去除一样)和内 源pyr4的同时诱变以产生无标记的pyr4-菌株。除了双重标记外，第一删除 质粒也包含原始KEX2的过表达盒(tre123561；启动子cDNA1,终止子cbh2)。
用于天冬氨酸蛋白酶pep2(tre0053961)的第二删除质粒pTTv232使用如 上的质粒pTTv213作为骨架构建。kex2过表达盒(pcDNA1-kex2-tcbh2)利用AscI 消化从pTTv213去除。质粒pTTv232的克隆使用T4 DNA连接酶在室温下通 过标准连接反应(自身连接)进行。连接混合物的部分利用电穿孔转化到大 肠杆菌中。培养几个克隆，分离和消化质粒DNA以使用标准实验室方法筛 选正确连接。正确的连接进一步通过测序验证。
用于天冬氨酸蛋白酶pep2(tre0053961)的第三删除质粒pTTv246使用如 上的质粒pTTv232作为骨架构建。pyr4-hph双重标记使用NotI消化从pTTv232 去除。pyr4标记基因使用NotI消化从pTTv181(以上Δpep4-py4r)获得。质粒 pTTv246的克隆使用T4 DNA连接酶在室温下通过标准连接反应进行。连接 混合物的部分利用电穿孔转化到大肠杆菌中。培养几个克隆，分离和消化质 粒DNA以使用标准实验室方法筛选正确连接。标记的正确连接和定向进一 步通过测序验证。
1000bp的5'侧翼区域和1020bp的3'侧翼区域选择作为pep2删除质粒的 基础。从pep2 5'侧翼末端的300bp延伸用作同向重复片段。这些片段以及第 二选择标记盒(hph)、cDNA1启动子、原始kex2基因和cbh2终止子利用表14.1 中所列的引物通过PCR扩增。产物用琼脂糖凝胶电泳分离且正确的片段使用 标准实验室方法用凝胶提取试剂盒(Qiagen)从凝胶分离。pyr4选择标记利用 NotI消化从pTTv181(以上Δpep4-pyr4)获得。为能够实现pTTv213中完整双 重标记盒的去除，NotI限制性位点引入双重标记盒的两侧，且SwaI位点引入 两个选择标记之间。AscI位点引入kex2过表达盒的两侧(pep2 5'同向重复和 3'侧翼之间)。载体骨架是如实施例1中的EcoRI/XhoI消化的pRS426。质粒 pTTv213使用实施例1中描述的酵母同源重组方法构建。用于pep2的这些删 除质粒(pTTv213、pTTv232和pTTv246，表14.1)导致pep2基因座中的1580bp 删除并覆盖PEP2的完整编码序列。
表14.1：用于产生pep2删除质粒的引物
删除质粒pTTv213,载体骨架pRS426
删除质粒pTTv232
删除质粒pTTv246
具有pep2(tre53961)的9重蛋白酶删除菌株M574的产生
为产生无标记的9重蛋白酶删除菌株，pyr4标记的去除基本上如实施例 3中对于从菌株M195(Δpep1)去除pyr4 blaster盒所述应用于8重删除菌株 M504(38-48A,M496中的pTTv229)。完成相继的5-FOA选择步骤以确保所 选择的克隆源自单细胞。
最终克隆采用标准实验室方法通过用表14.2中所列的引物的PCR验证。 对于大多数克隆获得对应于blaster盒成功去除的信号。blaster盒的去除进一 步通过将克隆接种到有或没有5mM尿苷的最低培养基板上验证。在无尿苷 补充的板上没有观察到生长。用于产生9重蛋白酶删除菌株的所得菌株以菌 株编号M521命名。
为移除载体序列，质粒pTTv246(Δpep2-pyr4)用MssI消化并且正确的片 段使用QIAquick凝胶提取试剂盒(Qiagen)从琼脂糖凝胶纯化。大约5μg的删 除盒用于转化8重蛋白酶删除菌株M521 (Δpep1Δtsp1Δslp1Δgap1Δgap2Δpep4Δpep3Δpep5,pyr4-)的克隆。原生质体的制 备和转化基本上如实施例1中对于菌株M181和M195所述的使用pyr4选择进行。
转化体挑取作为第一划线。生长的划线通过PCR(使用表14.2中所列的 引物)筛选正确整合。给出预期的信号的克隆纯化为单细胞克隆并使用表14.2 中所列的引物通过PCR重筛选。pep2的删除采用实施例1中所描述的方法通 过选择的克隆的DNA印迹分析(数据未显示)确认。克隆41-45G用菌株编 号M574命名。
表14.2：用于筛选pyr4 blaster盒从8重蛋白酶删除菌株的去除和用于筛选pTTv246/Δpep2-pyr4整合和菌株纯度的引物
(62)用于筛选pyr4 blaster盒从M504的去除和菌株纯度
用于筛选pTTv246(Δpep2-pyr4)的整合
用于筛选pep2(tre53961)ORF的删除
具有删除Δpep1Δtsp1Δslp1Δgap1Δgap2Δpep4Δpep3Δpep5Δpep12的9重 蛋白酶删除菌株的产生
pep12删除质粒的产生
用于天冬氨酸蛋白酶pep12(tre119876)的第一删除质粒pTTv209基本上 如实施例1中对于Δpep1质粒pTTv41所述构建，但另外的第二选择标记盒 (携带链霉菌种的草胺膦N-乙酰转移酶基因(bar)的合成构建体)置于pyr4 基因之后从而产生具有双重选择标记blaster盒的删除质粒。双重标记系统使 得能够a)利用例如bar作为初始抗性标记和较快地选择；b)转化pyr4+菌株 (在转化前不需要产生pyr4-)；和c)使用5-氟乳清酸从转化体去除两个标记 (像标准pyr4 blaster盒的去除一样)和内源pyr4的同时诱变以产生无标记的pyr4-菌株。
用于天冬氨酸蛋白酶pep12(tre119876)的第二删除质粒pTTv245使用如 上的质粒pTTv209作为骨架构建。pyr4-bar双重标记利用NotI消化从pTTv209 去除。新的pyr4标记基因利用NotI消化从pTTv181(以上Δpep4-pyr4)获得。 质粒pTTv245的克隆使用T4DNA连接酶在室温下通过标准连接反应进行。 连接混合物的部分利用电穿孔转化到大肠杆菌中。培养几个克隆，分离和消 化质粒DNA以使用标准实验室方法筛选正确连接。标记的正确连接和定向 进一步通过测序验证。
1019bp的5'侧翼区域和895bp的3'侧翼区域选择作为pep12删除质粒的 基础。从pep1 2 5'侧翼末端的300bp延伸用作同向重复片段。这些片段利用 表14.3中所列的引物通过PCR扩增。产物用琼脂糖凝胶电泳分离且正确的 片段使用标准实验室方法用凝胶提取试剂盒(Qiagen)从凝胶分离。双重标记 (pyr4-bar)利用NotI从pTTv202(Δpep5-pyr4-bar)消化。为能够实现完整双重 标记盒的去除，NotI限制性位点引入双重标记盒的两侧。AscI位点引入pep12 5'同向重复和3'侧翼之间。载体骨架是如实施例1中EcoRI/XhoI消化的 pRS426。质粒pTTv209使用实施例1中描述的酵母同源重组方法构建。用 于pep12的这些删除质粒(pTTv209和pTTv245，表14.3)导致pep12基因座中 的2198bp删除并覆盖PEP 12的完整编码序列。
表14.3：用于产生pep12删除质粒的引物
删除质粒pTTv209(Δpep12-pyr4-bar),载体骨架pRS426
删除质粒pTTv245(Δpep12-pyr4)
具有pep12(tre119876)的9重蛋白酶删除菌株M575的产生
为产生无标记的9重蛋白酶删除菌株，pyr4标记的去除基本上如实施例 3中对于从菌株M195(Δpep1)去除pyr4 blaster盒所述应用于8重删除菌株 M504(38-48A，M496中的pTTv229)。完成相继的5-FOA选择步骤以确保所 选择的克隆源自单细胞。
最终克隆采用标准实验室方法通过用表14.4中所列的引物的PCR验证。 对于大多数克隆获得对应于blaster盒成功去除的信号。blaster盒的去除进一 步通过在有或没有5mM尿苷的情况下将克隆接种到最低培养基板上验证。 在无尿苷补充的板上没有观察到生长。用于产生9重蛋白酶删除菌株的所得 菌株以菌株编号M521命名。
为移除载体序列，质粒pTTv245(Δpep1 2-pyr4)用MssI消化并且正确的 片段使用QIAquick凝胶提取试剂盒(Qiagen)从琼脂糖凝胶纯化。大约5μg的 删除盒用于转化8重蛋白酶删除菌株M521 (Δpep1Δtsp1Δslp1Δgap1Δgap2Δpep4Δpep3Δpep5,pyr4-)的克隆。原生质体的制 备和转化基本上如实施例1中对于菌株M181和M195所述的使用pyr4选择 进行。
转化体挑取作为第一划线。生长的划线通过PCR(使用表14.4中所列的 引物)筛选正确整合。给出预期的信号的克隆纯化为单细胞克隆并使用表14.4 中所列的引物通过PCR重筛选。pep12的删除采用实施例1中所描述的方法 通过选择的克隆的DNA印迹分析(数据未显示)确认。克隆42-45B以菌株 编号M575命名。
表14.4：用于筛选pyr4 blaster盒从8重菌株的去除和用于筛选 pTTv245/Δpep12-pyr4整合和菌株纯度的引物
(63)用于筛选pyr4 blaster盒从M504的去除和菌株纯度
用于筛选pTTv245(Δpep12-pyr4)的整合
用于筛选pep12(tre119876)ORF的删除
实施例15-10重蛋白酶删除菌株的产生
具有删除Δpep1Δtsp1Δslp1Δgap1Δgap2Δpep4Δpep3Δpep5Δpep2Δpep11 的10重蛋白酶删除菌株的产生
pep11删除质粒的产生
用于天冬氨酸蛋白酶pep11(tre121306)的删除质粒pTTv312基本上如实 施例1中对于Δpep1质粒pTTv41所述构建。
956bp的5'侧翼区域和943bp的3'侧翼区域选择作为pep11删除质粒的 基础。从pep115'侧翼末端的307bp延伸用作同向重复片段。这些片段利用 表15.1中所列的引物通过PCR扩增。产物用琼脂糖凝胶电泳分离且正确的 片段使用标准实验室方法用凝胶提取试剂盒(Qiagen)从凝胶分离。pyr4盒利 用NotI消化从pTTv181(以上Δpep4-pyr4)获得。为能够实现标记盒的去除， NotI限制性位点引入盒的两侧。AscI位点引入pep115'同向重复和3'侧翼之间。 载体骨架是如实施例1中的EcoRI/XhoI消化的pRS426。质粒使用实施例1 中描述的酵母同源重组方法构建。用于pep11的这一删除质粒(pTTv312，表 15.1)导致pep11基因座中的2624bp删除并覆盖PEP11的完整编码序列。
表15.1：用于产生pep11删除质粒的引物
(64)删除质粒pTTv312(&pep11-pyr4),载体骨架pRS426
具有pep11(tre121306)的10重蛋白酶删除菌株M658的产生
为产生无标记的10重蛋白酶删除菌株，pyr4标记的去除基本上如实施例 3中对于从菌株M195(Δpep1)去除pyr4 blaster盒所述应用于9重删除菌株 M574(41-45G,M521中的pTTv246)。完成相继的5-FOA选择步骤以确保所 选择的克隆源自单细胞。
最终克隆采用标准实验室方法通过用表15.2中所列的引物的PCR验证。 对于大多数克隆获得对应于blaster盒成功去除的信号。blaster盒的去除进一 步通过在有或没有5mM尿苷的情况下将克隆接种到最低培养基板上验证。 在无尿苷补充的板上没有观察到生长。用于产生10重蛋白酶删除菌株的所得 菌株以菌株编号M597命名。
为移除载体序列，质粒pTTv312(Δpep11-pyr4)用MssI消化并且正确的片 段使用QIAquick凝胶提取试剂盒(Qiagen)从琼脂糖凝胶纯化。大约5μg的删 除盒用于转化9重蛋白酶删除菌株M597 (Δpep1Δtsp1Δslp1Δgap1Δgap2Δpep4Δpep3Δpep5Δpep2,pyr4-)的克隆。原生质 体的制备和转化基本上如实施例1中对于菌株M181和M195所述的使用pyr4 选择进行。
转化体挑取作为第一划线。生长的划线通过PCR(使用表15.2中所列的 引物)筛选正确整合。给出预期的信号的克隆纯化为单细胞克隆并使用表15.2 中所列的引物通过PCR重筛选。pep11的删除采用实施例1中所描述的方法 通过选择的克隆的DNA印迹分析(数据未显示)确认。克隆47-62B用菌株 编号M632命名。附加的单细胞纯化步骤应用于菌株M632以获得10重蛋白 酶删除菌株M658。
表15.2：用于筛选pyr4 blaster盒从9重蛋白酶删除菌株的去除和用于筛选pTTv312/Δpep11-pyr4整合和菌株纯度的引物
(65)用于筛选pyr4 blaster盒从M574的去除和菌株纯度
用于筛选pTTv312(Δpep11-pyr4)的整合
用于筛选pep11 ORF的删除
实施例16-TPP1删除质粒的产生
用于三肽基肽酶tpp1(tre82623)的删除质粒pTTv331基本上如实施例1 中对于Δpep1删除质粒pTTv41所述构建，除了用于选择的标记是双重标记 pyr4-hph。
1245bp的5'侧翼区域和1025bp的3'侧翼区域选择作为tpp1删除质粒的 基础。从tpp1 5'侧翼末端的311bp延伸用作同向重复片段。这些片段使用表 16.1中所列的引物通过PCR扩增。产物用琼脂糖凝胶电泳分离且正确的片段 使用标准实验室方法利用凝胶提取试剂盒(Qiagen)从凝胶分离。pyr4-hph盒用 NotI消化从pTTv210(Δsep1-pyr4-hph)获得。为能够实现完整双重标记盒的去 除，NotI限制性位点引入双重标记盒的两侧。AscI位点引入tpp1 5'同向重复 和3'侧翼之间。载体骨架是如实施例1中的EcoRI/XhoI消化的pRS426。质 粒使用实施例1中描述的酵母同源重组方法构建。用于tpp1的这一删除质粒 (pTTv331，表16.1)导致tpp1基因座中的2152bp删除并覆盖TPP1的完整编 码序列。
表16.1：用于产生tpp1删除质粒的引物
(66)删除质粒pTTv331(Δtpp1-pyr4-hph),载体骨架pRS426
实施例17-PEP8删除质粒的产生
用于天冬氨酸蛋白酶pep8(tre122076)的另一删除质粒pTTv319基本上如 实施例1中对于pep1删除质粒pTTv41所述构建。
用于天冬氨酸蛋白酶pep8(tre122076)的第二删除质粒pTTv328使用如上 的质粒pTTv319作为骨架构建。pyr4标记利用NotI消化从pTTv319去除。 pyr4-hph盒利用NotI消化从pTTv210(Δsep1-pyr4-hph)获得。质粒pTTv328 的克隆使用T4DNA连接酶在室温下通过标准连接反应进行。连接混合物的 部分利用电穿孔转化到大肠杆菌中。培养几个克隆，分离和消化质粒DNA 以使用标准实验室方法筛选正确连接。标记的正确连接和定向进一步通过测 序验证。
1095bp的5'侧翼区域和988bp的3'侧翼区域选择作为pep8删除质粒的 基础。从pep8 5'侧翼末端的324bp延伸用作同向重复片段。这些片段利用表 17.1中所列的引物通过PCR扩增。产物用琼脂糖凝胶电泳分离且正确的片段 使用标准实验室方法用凝胶提取试剂盒(Qiagen)从凝胶分离。用于pTTv319 中的pyr4选择标记利用NotI消化从pTTv181(以上Δpep4-pyr4)获得。为能够 实现pyr4标记盒的去除，NotI限制性位点引入盒的两侧。AscI位点引入pep8 5'同向重复和3'侧翼之间。载体骨架是如实施例1中的EcoRI/XhoI消化的pRS426。质粒pTTv319使用实施例1中描述的酵母同源重组方法构建。用于pep8的这些删除质粒(pTTv319和pTTv328，表17.1)导致pep8基因座中的1543 bp删除并覆盖PEP8的完整编码序列。
表17.1：用于产生pep8删除质粒的引物
(67)删除质粒pTTv319(Δpep8-pyr4),载体骨架pRS426
删除质粒pTTv328(Δpep8-pyr4-hph)
用于天冬氨酸蛋白酶pep8(tre122076)的第三删除质粒pTTv266基本上如 实施例1中对于pep1删除质粒pTTv41所述构建，除了用于选择的标记是来 自pTTv194的双重标记pyr4-hph。
1095bp的5'侧翼区域和988bp的3'侧翼区域选择作为pep8删除质粒 pTTv266的基础。这些片段使用表17.2中所列的引物通过PCR扩增。产物用 琼脂糖凝胶电泳分离且正确的片段使用标准实验室方法利用凝胶提取试剂盒 (Qiagen)从凝胶分离。用于pTTv266中的pyr4-hgh选择标记用NotI消化从 pTTv194(以上Δpep4-pyr-hgh)获得。为能够实现pyr4-hgh标记盒的去除，NotI 限制性位点引入盒的两侧。载体骨架是如实施例1中的EcoRI/XhoI消化的 pRS426。质粒pTTv266使用实施例1中描述的酵母同源重组方法用5'侧翼、 3'侧翼、pyr4-hgh标记和载体骨架构建。用于pep8的删除质粒(pTTv266，表 17.2)导致pep8基因座中的1543bp删除并覆盖PEP8的完整编码序列。
表17.2：用于产生pep8删除质粒的引物
(68)删除质粒pTTv266(Δpep8-pyr4-hgh),载体骨架pRS426
实施例18–在干扰素产生菌株中蛋白酶删除的产生
产生IFN-α2b的5重蛋白酶删除菌株的产生
为产生用于五重蛋白酶删除菌株的IFN-α2b产生菌株， Δpep1Δtsp1Δslp1Δgap1Δgap2五重蛋白酶删除菌株M369使用潮霉素选择以 IFN-α2b表达盒(pTTv173)转化。具有Δpep1Δtsp1Δslp1Δgap1Δgap2五重蛋白 酶删除的这一IFN-α2b菌株以编号M401命名。
产生IFN-α2b的5重蛋白酶删除菌株M401的分析
为研究IFN-α2b的表达水平，5重蛋白酶删除 (Δpep1Δtsp1Δslp1Δgap1Δgap2)IFN-α2b产生菌株M401在培养条件pH 4.5； 28->22℃；30g/l葡萄糖,60g/l乳糖,20g/l全废谷物和20g/l废谷物提取物下 培养。为分析IFN-α2b的表达水平，第3天培养样品进行定量免疫印迹(图54A)。样品通过与IFN-α2b标准曲线比较进行分析且光密度定量用Totallab Quant TL100软件进行。免疫印迹用在TBST中稀释到1μg/ml的Abcam (#ab9386)抗IFN-α2b抗体进行。来自Bio-rad的二级抗体(#170-6520)山羊抗小 鼠IgG AP偶联二级抗体在TBST中1:5000稀释。对应于50ng、100ng、200 ng和400ng的IFN-α2b的蛋白质标准加载到凝胶上。分析显示M401以最高 51.9mg/l的产率产生IFN-α2b且52％的产物从载体分子切割。
IFN-α2b产生8重蛋白酶删除菌株M577的产生
为产生用于八重蛋白酶删除菌株的IFN-α2b产生菌株， Δpep1Δtsp1Δslp1Δgap1Δgap2Δpep4Δpep3Δpep5八重蛋白酶删除菌株M504使 用乙酰胺选择以IFN-α2b表达盒(pTTv254)转化。具有 Δpep1Δtsp1Δslp1Δgap1Δgap2Δpep4Δpep3Δpep5八重蛋白酶删除的这一 IFN-α2b菌株以编号M577命名。
产生IFN-α2b的8重蛋白酶删除菌株M577的分析
为研究IFN-α2b的表达水平，8重蛋白酶删除 (Δpep1Δtsp1Δslp1Δgap1Δgap2Δpep4Δpep3Δpep5)IFN-α2b产生菌株M577在 条件pH 4.5；28->22℃；2％酵母提取物、4％纤维素、8％纤维二糖和4％山梨糖 中培养。为研究IFN-α2b的表达，M577发酵样品进行免疫印迹(图54B)。为 分析IFN-α2b的表达水平，第4天培养样品进行定量免疫印迹(图55)。样品 通过与IFN-α2b标准曲线比较进行分析且光密度定量用Totallab Quant TL100 软件进行。免疫印迹用在TBST中稀释到1μg/ml的Abcam(#ab9386)抗 IFN-α2b抗体进行。来自Bio-rad的二级抗体(#170-6520)山羊抗小鼠IgG AP 偶联二级抗体在TBST中1:5000稀释。对应于50ng、100ng和200ng的 IFN-α2b的蛋白质标准加载到凝胶上。分析显示M577以最高1780mg/l的产 率产生IFN-α2b且66.5％的产物从载体分子切割。8重蛋白酶删除菌株M577产生比5重蛋白酶删除菌株M401多34倍的IFN-α2b。
产生IFN-α2b的9重蛋白酶删除菌株M652的产生
为产生用于九重蛋白酶删除菌株的IFN-α2b产生菌株， Δpep1Δtsp1Δslp1Δgap1Δgap2Δpep4Δpep3Δpep5Δpep2九重蛋白酶删除菌株 M574使用潮霉素选择以IFN-α2b表达盒(pTTv173)转化。具有 Δpep1Δtsp1Δslp1Δgap1Δgap2Δpep4Δpep3Δpep5Δpep2九重蛋白酶删除的这一 IFN-α2b菌株以编号M652命名。
产生IFN-α2b的9重蛋白酶删除菌株M652的分析
为研究IFN-α2b的表达水平，8重蛋白酶删除 (Δpep1Δtsp1Δslp1Δgap1Δgap2Δpep4Δpep3Δpep5Δpep2)IFN-α2b产生菌株 M652在条件pH 4.5；28->22℃；2％酵母提取物、4％纤维素、8％纤维二糖和 4％山梨糖下培养。为研究IFN-α2b的表达，M652发酵样品进行免疫印迹(图54B)。为分析IFN-α2b的表达水平，第3天培养样品进行定量免疫印迹(图55)。 样品通过与IFN-α2b标准曲线比较进行分析且光密度定量用Totallab Quant TL100软件进行。免疫印迹用在TBST中稀释到1μg/ml的Abcam(#ab9386) 抗IFN-α2b抗体进行。来自Bio-rad的二级抗体(#170-6520)山羊抗小鼠IgG AP 偶联二级抗体在TBST中1:5000稀释。对应于50ng、100ng和200ng的 IFN-α2b的蛋白质标准加载到凝胶上。分析显示M652以最高1928mg/l的产 率产生IFN-α2b且85％的产物从载体分子切割。9重蛋白酶删除菌株M652产生比8重蛋白酶删除菌株M577略多的IFN-α2b和比5重蛋白酶删除菌株 M401多37倍的IFN-α2b。
pep8(tre122076)从干扰素产生菌株M577删除的9重蛋白酶删除菌株M670 的产生
为除去删除盒，质粒pTTv266(Δpep8-pyr4-hgh)用PmeI消化且正确的片 段使用QIAquick凝胶提取试剂盒(Qiagen)纯化。大约5μg的删除盒用于转化 8重蛋白酶删除菌株M577(Δpep1Δtsp1Δslp1Δgap1Δgap2Δpep4Δpep3Δpep5)。 M577菌株产生干扰素α2b。原生质体的制备和转化基本上如实施例1中所 述的使用潮霉素选择进行。
挑取转化体并在选择板上划线。生长的划线通过PCR(使用表18.1中所 列的引物)筛选正确整合。给出预期的信号的克隆纯化为单细胞克隆并使用 表18.1中所列的引物通过PCR重筛选。克隆82-9用菌株编号M670命名。
表18.1：用于筛选pTTv266/Δpep8-pyr4-hgh整合和菌株纯度的引物
(69)用于筛选pTTv266(Δpep8-pyr4-hgh)的整合
用于筛选pep8 ORF的删除
pep11删除质粒的产生
用于天冬氨酸蛋白酶pep11(tre121306)的删除质粒pTTv268基本上如实 施例1中对于pep1删除质粒所述构建，除了用于选择的标记是来自pTTv194 的pyr4-hph。
956bp的5'侧翼区域和957bp的3'侧翼区域选择作为pep11删除质粒 pTTv268的基础。这些片段使用表18.2中所列的引物通过PCR扩增。产物用 琼脂糖凝胶电泳分离且正确的片段使用标准实验室方法利用凝胶提取试剂盒 (Qiagen)从凝胶分离。pyr4-hgh盒用NotI消化从pTTv194(Δpep4-pyr-hgh)获 得。为能够实现标记盒的去除，NotI限制性位点引入盒的两侧。载体骨架是 如实施例1中的EcoRI/XhoI消化的pRS426。构建了质粒。质粒pTTv268使 用实施例1中描述的酵母同源重组方法用5'侧翼、3'侧翼、pyr4-hgh标记和载体骨架构建。用于pep11的这一删除质粒(pTTv268，表18.2)导致pep11基因 座中的删除并覆盖PEP11的完整编码序列。
表18.2：用于产生pep11删除质粒的引物
(70)删除质粒pTTv268(Δpep11-pyr4-hgh),载体骨架pRS426
(71)
从干扰素产生菌株M577删除pep11(tre121306)的9重蛋白酶删除菌株M672 的产生
为除去删除盒，质粒pTTv268(Δpep11-pyr4-hgh)用PmeI消化且正确的片 段使用QIAquick凝胶提取试剂盒(Qiagen)纯化。大约5μg的删除盒用于转化 8重蛋白酶删除菌株M577(Δpep1Δtsp1Δslp1Δgap1Δgap2Δpep4Δpep3Δpep5)。M577菌株产生干扰素α2b。原生质体的制备和转化基本上如实施例1中所 述的使用潮霉素选择进行。
挑取转化体并在选择板上划线。生长的划线通过PCR(使用表18.3中所 列的引物)筛选正确整合。给出预期的信号的克隆纯化为单细胞克隆并使用 表18.3中所列的引物通过PCR重筛选。克隆33-9用菌株编号M672命名。
表18.3：用于筛选pTTv268/Δpep11-pyr4-hgh整合和菌株纯度的引物
(72)用于筛选pTTv268(Δpep11-pyr4-hgh)的整合
用于筛选pep11 ORF的删除
从干扰素产生菌株M577删除slp7(tre123865)的9重蛋白酶删除菌株M673 的产生
为除去删除盒，质粒pTTv269(Δslp7-pyr4-hgh)用PmeI消化且正确的片 段使用QIAquick凝胶提取试剂盒(Qiagen)纯化。大约5μg的删除盒用于转化 8重蛋白酶删除菌株M577(Δpep1Δtsp1Δslp1Δgap1Δgap2Δpep4Δpep3Δpep5)。 M577菌株产生干扰素α2b。原生质体的制备和转化基本上如实施例1中所 述的使用潮霉素选择进行。
挑取转化体并在选择板上划线。生长的划线通过PCR(使用表18.4中所 列的引物)筛选正确整合。给出预期的信号的克隆纯化为单细胞克隆并使用 表18.4中所列的引物通过PCR重筛选。克隆5-64用菌株编号M673命名。
表18.4：用于筛选pTTv269/Δslp7-pyr4-hgh整合和菌株纯度的引物
(73)用于筛选pTTv269(Δslp7-pyr4-hgh)的整合
用于筛选slp7 ORF的删除
实施例19–G0产生菌株M627和M629的产生
载体pTTg156和pTTg173通过向中间载体pTTg145和pTTg146添加双 重选择标记盒(pyr4表达盒之外的pki1启动子和cbh1终止子之间的潮霉素抗 性标记基因(hph))构建。中间体通过酵母重组克隆构建，标记盒通过常规克隆 利用NotI消化和连接添加。
用于中间载体的片段的产生策略在以下表19.1中给出。用于片段产生的 引物列于以下表19.2中。一旦用于pTTg145和pTTg146的片段按照表19.1 中的方案构建，它们进行电穿孔到酿酒酵母FY834中用于通过同源重组进行 质粒组装。酵母细胞接种到SC-ura在+30℃下培养2-3天。然后集落从板汇 集且质粒池用苯酚/氯仿提取方法纯化。质粒池通过电穿孔转化到感受态大肠 杆菌细胞中。电穿孔的细胞接种到LB+amp选择板上，在+37℃下培养过夜， 且集落通过PCR进行筛选。阳性集落然后作为纯培养划线到新板上，单一集 落在液体LB+amp培养基中培养且潜在中间载体pTTg145和pTTg146按照标 准方案纯化。质粒通过限制性酶切分析进行分析且序列通过测序验证。
标记盒然后通过从pTTg163常规NotI消化和连接到NotI线性化的中间 体pTTg145和pTTg146中而添加到载体。
表19.1：中间载体pTTg145-pTTg149的构建策略。pTTv141和pTTv11描述 于国际专利申请No.PCT/EP2011/070956中。质粒pTTg124和pTTv225用 于下表中指定的片段的构建。
表19.2：用于构建中间载体pTT145-pTTg149的引物
具有7重蛋白酶删除的里氏木霉MAB01表达菌株M507用靶向于alg3 基因座的载体pTTg156和pTTg173的PmeI片段转化。挑取可变量的转化体 (100-170个，取决于构建体)到选择板上。基于Phire Plant Direct PCR试剂盒 (Finnzymes F-130)的PCR筛选，具有关于5'-和3'-整合的阳性结果的克隆选择 用于单孢子接种及再筛选整合和alg3删除(来自pTTg156转化的5个克隆， 来自pTTg173转化的3个克隆)。用于筛选的引物列于以下表19.3中。
PCR筛选的菌株最后进行摇瓶培养和聚糖分析。最终菌株命名为M629 (pTTg173转化)和M627(pTTg156转化)。
表19.3：用于筛选M507 pTTg156和pTTg173中的alg3整合的引物
菌株M627和M629的WSG发酵和聚糖分析
里氏木霉菌株M627和M629在4％WSG、2％葡萄糖、4％纤维二糖、 6％乳糖,pH 5.5中发酵，且在第3-6天进行取样。抗体滴度显示于表19.4中。
对于N-聚糖分析，MAB01使用Protein G HP MultiTrap 96孔过滤板(GEHealthcare)按照制造商的说明从培养物上清液纯化。抗体浓度通过UV吸光度 针对MAB 01标准曲线测定。
N-聚糖在20mM磷酸钠缓冲液,pH 7.3中在+37℃的过夜反应中使用 PNGase F(ProZyme Inc.)从EtOH沉淀和SDS变性的抗体释放。释放的N-聚 糖用Hypersep C-18和Hypersep Hypercarb(Thermo Scientific)纯化并用MALDI-TOF MS进行分析。结果显示于表19.6中。在菌株M627和M629中， G0水平的范围为24.3％-41.7％，在菌株M507中没有看到G0。
菌株M627和M629的摇瓶中的WSG培养及聚糖分析
里氏木霉菌株M627和M629在TrMM,4％乳糖,2％SGE,100mM PIPPS, pH 5.5中+28℃下摇瓶中进行培养。在第5天进行取样。
对于N-聚糖分析，MAB01使用Protein G HP MultiTrap 96-孔过滤板(GEHealthcare)按照制造商的说明从培养物上清液纯化。蛋白G洗脱液中的抗体 浓度通过UV吸光度针对MAB 01标准曲线测定(表19.5)。培养基中的滴度不 测量。
N-聚糖释放如上进行，结果显示于表19.7中。G0水平对于M627和M629 分别是21.1和56.9％。
表19.4：在WSG培养基中发酵的菌株M627和M629的发酵培养物上清液 中的抗体滴度
表19.5：摇瓶中WSG培养基中培养的菌株M627和M629的蛋白G洗脱液 中的抗体浓度
菌株 蛋白G洗脱液中的滴度(g/l)
M507 0.303
M627 0.105
M629 0.103
表19.6：来自WSG培养基中发酵的菌株M627和M629的纯化抗体中的中 性N-聚糖的相对比例。在第3-6天取样。
表19.7：摇瓶中WSG培养基中培养的菌株M627和M629在第5天的纯化 抗体的中性N-聚糖的相对比例。
实施例20—具有不同启动子的GLCNACMAN5产生菌株的产生
具有不同启动子的用于GnTI的载体描述于表20.1中。载体靶向于里氏木 霉的egl2基因座中。
表20.1：具有不同启动子的人GnTI载体的描述。
pTTg153 对于Egl2的pcDNA1-Kre2-GnTI
pTTg167 对于Egl2的pcbh1-Kre2-GnTI
pTTg168 对于Egl2的pgpdA-Kre2-GnTI
pTTg170 对于Egl2的pgpdA-GnTI
pTTg171 对于Egl2的pcbh1-Kre2-GnTI(无活性的GnTI)
材料和方法
用于表20.1的载体的片段的产生策略在表20.2中给出且用于片段产生的 引物列于表20.3中。片段通过PCR扩增且产物从琼脂糖凝胶纯化。消化的 pTTg152载体从凝胶纯化。所有PCR扩增用高保真Phusion聚合酶(Finnzymes) 进行。用于pTTg153-pTTg171的片段电穿孔到酿酒酵母FY834中用于通过同 源重组进行质粒组装。酵母细胞接种到SC-ura在+30℃下培养2-3天。然后 集落从板汇集且质粒池常规地用苯酚/氯仿提取方法纯化。质粒池通过电穿孔 转化到感受态大肠杆菌细胞中。电穿孔的细胞接种到LB+amp选择板上，在 +37℃下培养过夜，且集落通过PCR筛选。阳性集落然后作为纯培养划线到 新板上，单一集落在液体LB+amp培养基中培养且潜在载体 pTTg153-pTTg171按照标准方案纯化。质粒通过限制性酶切分析进行分析且 序列通过测序验证。
表20.2：载体的构建策略。载体pTTv225、pTTv77、pTTg91用于构建下面 指定的片段。载体pTTv110、pTTv141,pTTv11和pTTv13在国际专利申请 No.PCT/EP2011/070956中描述。
表20.3：用于构建表20.2的载体的引物
具有7重蛋白酶删除的里氏木霉MAB01表达菌株M507用靶向于egl2 基因座的载体pTTg153-pTTg171的PmeI片段转化。挑取可变量的转化体到 选择板上。基于Phire PlantDirect PCR试剂盒(Finnzymes F-130)的PCR筛选， 具有关于5'-和3'-整合的阳性结果的克隆选择用于单孢子接种及再筛选整合 和egl2删除。PCR筛选的菌株最后进行摇瓶培养和聚糖分析。
用GnTI和启动子构建体转化的菌株M507的摇瓶培养
用表20.1和20.2的载体转化的菌株M507在+28℃下TrMM,4％乳糖,2％ SGE,100mMPIPPS,pH5.5中在摇瓶中培养，且在第5天进行取样。测试无 活性的GnTI构建体以确定GlcNAcMan5聚糖对里氏木霉生长的可能影响。
为了N-聚糖分析，纯化MAB01，如上所述测定浓度并用MALDI-TOF MS 分析N-聚糖。MAB01的N-聚糖分析表明GnMan5水平的范围为总聚糖的 8-79.2％(表20.4及20.5A和B)。如预期的，无活性GnTI产生野生型糖基化。
表20.4：GnTI构建体和抗体浓度。所选择克隆的菌株编号在“克隆”列中 的括号中给出。
表20.5A和B：第5天MAB01的中性N-聚糖的相对比例
用GnTI构建体转化的菌株M507的发酵培养
里氏木霉菌株M702、M704、M706、M710、M712、M716和M507在 4％WSG、2％Glc、4％纤维二糖、6％乳糖,pH 5.5中发酵，且在第3-6天进 行取样。抗体滴度显示于表20.6中。N-聚糖脱离并使用PNGase F如上所述 进行分析。
MAB01的N-聚糖分析表明GnMan5水平的范围为总聚糖的1.8-68.5％ (表20.7A、B和C)。无活性GnTI如预期的产生野生型糖基化，如在对照菌 株M507中。
表20.6：MAB01的抗体浓度
表20.7A、B和C：第3、4、5和6天MAB01上中性N-聚糖的相对比例
实施例21-具有不同靶向肽的GLCNACMAN5产生菌株的产生
质粒的产生
用于产生具有不同高尔基体靶向肽的GNTI菌株的质粒(pTTv274、 pTTv275、pTTv276、pTTv278、pTTv279、pTTv280)全部是基于共同的亲本 质粒pTTv265，其包含具有38个氨基酸N-末端的截短的人GNT1(P26572)。 pTTv265的系谱总结于表21.1中。
表21.1：高尔基体靶向肽质粒pTTv274、pTTv275、pTTv276、pTTv278、 pTTv279、pTTv280的系谱。高尔基体靶向肽添加到pTTv265上产生前述质 粒。
质粒产生的逐步说明
质粒pTTv77包含用于将整合靶向于里氏木霉基因组的egl2(tre120312) 5'和3'侧翼区域及用于基因表达的cbh1启动子。质粒pTTv77整合于基因组 导致egl2基因座中的2456bp删除。对于5'和3'侧翼扩增egl2基因座的1020 bp和1024bp区域。对于启动子片段扩增来自cbh1基因座的2176bp。用于 PCR反应中的模板是里氏木霉的基因组DNA。用于PCR反应中的引物显示 于表21.2中。选择标记pyr4 blaster盒是来自如上所述的pTTv71的NotI片段 和载体骨架是如上所述的EcoRI/XhoI消化的pRS426。所有片段使用标准实 验室方法纯化和质粒通过实施例中描述的酵母重组方法克隆。在质粒拯救到 大肠杆菌中后，几个克隆进行正确重组的验证。储存的克隆通过测序验证。
表21.2：用于克隆pTTv77的引物
质粒pTTv256是基于质粒pTTv77。在质粒pTTv256中，启动子cbh1变 为gpdA。为克隆pTTv256，质粒pTTv77用FseI/PacI消化以释放cbh1启动 子。新的启动子构巢曲霉gpdA用FseI/PacI消化从质粒释放。片段纯化和克 隆使用标准实验室方法进行。几个克隆进行正确连接的验证。储存的克隆通 过测序验证。
质粒pTTv264是基于质粒pTTv256。在质粒pTTv264中，选择标记从 pyr4 blaster盒变为hygR选择标记。为克隆pTTv264，质粒pTTv256用NotI 消化以释放pyr4 blaster盒。标记hygR使用修饰的pRLMex30作为模板用表 21.3中显示的引物通过PCR扩增。所有片段使用标准实验室方法纯化和质粒 通过实施例中描述的酵母重组方法克隆。在质粒拯救到大肠杆菌中后，几个
克隆进行正确重组的验证，且储存的克隆通过测序验证。
表21.3：用于克隆pTTv264的引物
质粒pTTv265是基于如上所述的质粒pTTv264。在pTTv265中，具有114 个核苷酸(38个氨基酸)的N-末端截短的人GNT1在gpdA启动子下添加。为 克隆pTTv265，质粒pTTv264用PacI线性化。人GNT1通过PCR从携带全 长人GNT1基因(P26572，pTTv11也描述于国际专利申请No. PCT/EP2011/070956中)的合成质粒pTTv11扩增。用于扩增的引物显示于表 21.4中。所有片段使用标准实验室方法纯化。质粒通过如上所述的酵母重组 方法克隆。在质粒拯救到大肠杆菌中后，几个克隆进行正确重组的验证，且 储存的克隆通过测序验证。
表21.4：用于克隆pTTv265的引物
用于构建pTTv265的质粒pTTv11中的人N-末端38氨基酸截短的GnTI 氨基酸序列。
SVSALDGDPASLTREVIRLAQDAEVELERQRGLLQQIGDALSSQRGRVPTAAPPAQPRVPVTPAPAVIPILVIACDRSTVRRCLDKLLHYRPSAELFPIIVSQDCGHEETAQAIASYGSAVTHIRQPDLSSIAVPPDHRKFQGYYKIARHYRWALGQVFRQFRFPAAVVVEDDLEVAPDFFEYFRATYPLLKADPSLWCVSAWNDNGKEQMVDASRPELLYRTDFFPGLGWLLLAELWAELEPKWPKAFWDDWMRRPEQRQGRACIRPEISRTMTFGRKGVSHGQFFDQHLKFIKLNQQFVHFTQLDLSYLQREAYDRDFLARVYGAPQLQVEKVRTNDRKELGEVRVQYTGRDSFKAFAKALGVMDDLKSGVPRAGYRGIVTFQFRGRRVHLAPPPTWEGYDP SWN
质粒pTTv274、pTTv275、pTTv276、pTTv278、pTTv279和pTTv280全 部基于如上所述的质粒pTTv265。在这些质粒中，不同高尔基体靶向肽添加 到N-末端截短的人GNT1基因之前。为克隆这些质粒，pTTv265用PacI线 性化。不同高尔基体靶向肽使用表21.5A中显示的引物通过PCR扩增。用于 GNT2的模板DNA(pTTv274)是携带国际专利申请No.PCT/EP2011/070956的 密码子调和的人GNT2基因的合成质粒。用于其它高尔基体靶向肽的模板(pTTv276、pTTv278、pTTv279、pTTv280)是里氏木霉基因组DNA。KRE2 (pTTv275)使用表21.5A中的退火引物通过PCR产生。所有片段使用标准实 验室方法纯化和质粒通过实施例中描述的酵母重组方法克隆。在质粒拯救到 大肠杆菌中后，几个克隆进行正确重组的验证，且储存的克隆通过测序验证。
表21.5A：用于产生pTTv274、pTTv275、pTTv276、pTTv278、pTTv279 和pTTv280中的高尔基体靶向肽的引物
表21.5B：表21.5A的质粒中靶向肽的氨基酸序列
具有GNTI的不同高尔基体靶向肽的菌株的产生
用于转化的片段用PmeI从质粒pTTv274(GNT2)、pTTv275(KRE2)、 pTTv276(KRE2-样)、pTTv278(OCH1)、pTTv279(ANP1)和pTTv280(VAN1) (表21.5A和21.5B)释放。所有片段单独地转化到MAB01表达菌株M507且 原生质体转化基本上如实施例中对于潮霉素选择所述进行。
在选择性划线上良好生长的克隆筛选egl2基因座中的5'和3'整合。双重 整合阳性克隆另外筛选egl2 ORF的缺失。给出所希望的结果的克隆通过单孢 子接种纯化，且单孢子来源的克隆通过PCR验证为纯整合菌株。所得菌株列 于以下表21.6中。
表21.6：GnTI高尔基体靶向肽菌株的概要
菌株M507、M607、M610、M615、M620、M622和M685的发酵和聚 糖分析
里氏木霉菌株M507、M607、M610、M615、M620、M622和M685在 4％全废谷物、2％葡萄糖、4％纤维二糖、6％乳糖,pH 5.5中发酵，且在第3-6 天进行取样。抗体滴度显示于表21.7中。N-聚糖脱离且如上所述使用PNGase F进行分析。
MAB01的N-聚糖分析显示GnMan5水平的范围为总聚糖的4-66％(表 21.8A、B和C)。对照菌株M507如预期的显示野生型糖基化。
表21.7：具有带不同靶向肽的人GnTI的菌株的抗体浓度
表21.8A、B和C：具有带不同靶向肽的人GnTI的菌株在第3、4、5和6 天MAB01的中性N-聚糖的相对比例
实施例22–通过RNAI和SLP2删除的SLP基因沉默
构建三个沉默构建体用于敲减slp2(tre123244)的表达。这些RNAi构建 体包含gpdA启动子(将整合靶向于pep2(tre53961)蛋白酶基因座)和具有3' pep2同向重复的apyr4环出标记。两个短的19bp靶序列和大的448bp序列 插入这一载体中以分别产生pTTv217、pTTv218和pTTv263。这些载体设计 为敲减slp2的表达并降低其蛋白酶活性。RNAi载体转化到pyr4-形式的 MAB01产生菌株M507中。pTTv204载体显示于图52中。
pTTv204 RNAi表达载体用AsiSI限制性酶线性化。引物T846和T847 一起退火并通过酵母重组整合到pTTv204载体中。引物显示于表22.1中。19 碱基对的靶序列包含在所得的pTTv217载体中。引物T848和T849一起退火 并整合到线性化的pTTv204载体中以产生pTTv218 RNAi载体。这一载体包 含19碱基对的靶序列。引物显示于表22.1中。靶序列显示于表22.2中。
pTTv263载体以两段形成并整合到pTTv204载体中。引物T965和T967 用于扩增包括slp2基因中的58碱基对内含子序列的506碱基对正义片段。 pTTv204载体用AsiSI限制性酶打开且506正义片段通过酵母重组整合到载 体中。引物T1006和T1007用于扩增448碱基对的反义片段。反义片段用FseI 和AscI限制性酶消化。包括正义片段的载体也用FseI和AscI消化。载体和 反义片段连接到一起以产生载体pTTv263。引物列于表22.1中。靶序列显示 于表22.2中。
pTTv217、pTTv218、pTTv263 RNAi载体用PmeI消化以释放表达盒。片 段用琼脂糖凝胶电泳分离且正确的片段使用标准实验室方法用凝胶提取试剂 盒(Qiagen)分离。大约5μg的表达盒用于转化MAB01抗体表达菌株M507 (pyr4-形式)。原生质体的制备和转化使用pyr4选择基本上如实施例1中对于 菌株M181和M195所述进行。
pTTv217载体中的短的靶序列设计用于特别地仅影响slp2。pTTv218靶 序列与slp3、slp5和slp6同源。pTTv263载体中的大的448bp靶序列意图影 响几种枯草溶菌素。这些载体中的靶序列列于表22.2中。所得的敲减菌株 M665、M666和M667在小规模培养中培养。
几种pTTv217、pTTv218和pTTv263转化体在24孔培养中生长以将其 MAB01产量与对照菌株M507进行比较。菌株在具有柠檬酸二铵(而没有硫 酸铵)、100mM PIPPS、2％废谷物提取物、4％乳糖的TrMM，pH 5.5中生长。 双重孔用于各转化体。在第6天获取的来自24孔的样品用于免疫印迹。上清 液用柠檬酸钠缓冲液pH 5.5稀释，以使得0.5μl的各上清液可以加载到 4-15％Criterion凝胶中。与LSB+BME混合并在95℃下加热5分钟。蛋白质 用100伏的Criterion印迹装置30分钟转移到硝基纤维素上。硝基纤维素膜用 TBST中的5％乳封闭1小时。重链用TBST中1:10,000稀释的抗重链AP偶 联抗体(Sigma#A3188)检测。在与检测抗体孵育1小时后，印迹用TBST洗涤 且膜用AP底物(Promega)显影。
结果可以在图53中看到。217.12G转化体与M507或第二转化体217.12E 相比产生略高的重链量。最显著的改善对于pTTv218转化体观察到。三个转 化体显著高于对照。218.25F是明显出众的。pTTv263转化体的结果是更多变 的。两个转化体产生非常少的抗体重链。转化体263.110F似乎产生对照两倍 多的重链。
靶向多个蛋白酶的构建体更成功地提高重链表达。总体上，pTTv218转 化体始终好于M507对照。在两个pTTv263转化体中未观察到产生表明RNAi 也发挥良好作用。当slp2基因删除时，菌株的生长受损害且因此抗体表达也 降低。263.36A和263.124C转化体生长非常不好且表达非常少的slp2。这通 过摇瓶和qPCR研究确认。
摇瓶培养的干重测量可以在表22.5中看到。菌株在具有柠檬酸二铵(而 没有硫酸铵)、100mM PIPPS、2％废谷物提取物、4％L乳糖的TrMM pH 5.5 中生长。重复的瓶用于各转化体。263.124C转化体具有生长的困难。一般地， 在所有表达RNAi的菌株中具有小的生长降低。这一效应可能与较低的slp2 表达水平相关。
为证实slp2表达事实上通过RNAi的表达降低，qPCR研究用摇瓶研究 菌丝体进行。RNA从摇瓶研究菌丝体纯化，合成cDNA，并进行qPCR分析。 slp2、slp3、slp5、slp6和gpd1表达用基因特异性的引物监测。倍数改变是针 对对照菌株测量。表达用gpd1标准化。
263.124C转化体显示slp2的最大下调(表22.6)。大的RNAi诱导slp2基 因的36倍下调，达到其几乎关闭的点。其它转化体显示温和得多的敲减活性， 范围为1.2-2.5倍。温和的敲减是更优选的，因为菌株生长得更好且可以产生 良好水平的抗体。
利用两种转化体，更密切地观察到其它哪些枯草溶菌素受RNAi表达的 影响。在263.124C转化体中，证明slp3和slp6也分别敲减6倍和2.3倍。对 于更温和敲减的菌株218.25F，slp2和slp3两者减少表达1.7和1.8倍。
包括Slp2删除的8重删除菌株M646的产生
M646 slp2删除菌株通过转化pTTv115删除盒到M564(M507的pyr4-形 式)中进行。M564pyr4-菌株基本上如实施例3中对于从菌株M195(Δpep1)去 除pyr4 blaster盒所述产生。进行相继5-FOA选择步骤以确保所选择的克隆源 自单细胞。
包含slp2侧翼和pyr4标记的删除盒通过PmeI消化从载体去除且正确的 片段使用QIAquick凝胶提取试剂盒(Qiagen)从琼脂糖凝胶纯化。大约5μg的 删除盒用于转化MAB01产生菌株M564 (Δpep1Δtsp1Δslp1Δgap1Δgap2Δpep4Δpep3,pyr4-)。原生质体的制备和转化基 本上如实施例1中对于菌株M181和M195所述使用pyr4选择进行。
转化体挑取作为第一划线。生长的划线通过PCR(使用表22.3中所列的 引物)筛选正确整合和slp2 ORF的缺失。给出预期的信号的克隆纯化为单细 胞克隆并使用表22.3中所列的引物通过PCR重筛选。正确的克隆命名为菌 株M646。
菌株M507、M665、M666、M667和M646的发酵
M507菌株在与M665、M666、M667和M646相同的条件下在发酵培养 系列FTR104中培养。M646是slp2删除菌株。M665、M666和M667是具有 RNAi沉默的菌株。FTR104培养在木霉属最低培养基(TrMM)加20g/L酵母 提取物、40g/L纤维素、80g/L纤维二糖和40g/L山梨糖，pH5.5中生长。 温度在48小时后从28℃转换到22℃。培养物生长6天。木霉属最低培养基 包含5g/L硫酸铵、5g/L磷酸二氢钾、1ml/L痕量元素、4.1ml的1M氯化钙 /L和2.4ml的1M硫酸镁/L培养基。
总抗体浓度从第3-6天测定。在第6天，M667菌株达到3.81g/L，参见 表22.8。在第5天后，抗体的表达在M507、M665和M666培养中下降。在 第6天，M507菌株产生2.2g/L，M665达到2.7g/L和M666产生2.8g/L。 因此具有小RNAi靶序列的菌株产生比M507略多的抗体，表明沉默在这些 菌株中发挥作用。具有slp2删除的菌株M646比其它菌株更慢地生长。slp2 删除菌株在第6天产量略高于2g/L。
菌株M507、M665、M666和M667的发酵
217.12G(M665)、218.25F(M666)和263.110F(M667)在具有30g/l葡萄糖、 60g/l乳糖、20g/l WSG、20g/l SGE加乳糖补料，pH 5.5的1L发酵罐中开 始在28℃下并随后在培养中转换到22℃下生长。MAB01重链和轻链表达 通过从培养的各天收集的上清液样品的免疫印迹进行分析。上清液在pH 5.5 的柠檬酸盐缓冲液中稀释以使得0.1μl可以加载到各孔。添加LSB+BME并 在95℃下一起加热5分钟。样品加载到4-15％Criterion SDS PAGE凝胶中。蛋白质用Criterion印迹装置以100伏30分钟转移到硝基纤维素上。硝基纤维 素膜用TBST中的5％乳封闭1小时。重链用TBST中1:10,000稀释的抗重链 (Sigma#A3188)和抗轻链(Sigma#A3813)AP偶联抗体检测。在与检测抗体孵 育1小时后，印迹用TBST洗涤，且膜用AP底物(Promega)显影。
总抗体表达在蛋白G纯化后测量且值在表22.4中连同两个对照菌株的结 果给出。M507菌株在相同的条件下在具有或没有SBTI抑制剂的情况下培养。 M667菌株中MAB01的表达水平高于M507亲本菌株中测量的表达水平。在 第9天，例如，表达水平为M667的两倍高。对于M667观察到的表达水平 类似于用添加SBTI进行的培养。M665和M666菌株产生略低于或类似于对 照的水平。与标准M507菌株相比，抗体表达明确地存在2倍的提高。
测量表22.4中所列的培养的蛋白酶活性以确定总蛋白酶活性如何受 RNAi影响。以酪蛋白作为底物的蛋白酶活性测量可以在表22.9中看到。测 量来自所有上清液样品的总蛋白质浓度。所有样品的总蛋白质浓度在柠檬酸 钠缓冲液pH 5.5中对于培养的所有天针对0.625mg/ml标准化。
100μl的所有稀释的上清液添加到96孔板中。每样品进行三个重复孔。 添加100μl在柠檬酸钠缓冲液pH 5.5中制备的酪蛋白FL稀释原液(10μg/ml)。 来自小瓶的酪蛋白原液是在200μl PBS中稀释的1000μg/ml。对于各样品， 包括100μl的稀释上清液和100μl的柠檬酸钠缓冲液pH 5.5的背景对照。在 37℃下孵育包含覆盖在塑料包中的上清液和底物的板。荧光在孵育4小时后 在板中测量。读数在荧光读板仪上利用485nm激发和530nm发射进行。
M665菌株的上清液中的蛋白酶活性总体上是最低的。在整个培养中， 它是M507的蛋白酶活性的几乎一半。大的发夹载体M667活性也是低的， 但它在第5天后开始降低且在第10天最低。这是第10天M667菌株的抗体 产量最高的情况。在培养结束时，M665和M667培养上清液两者与M507对 照相比具有一半的蛋白酶活性。当M507培养补充SBTI蛋白酶抑制剂时，蛋 白酶活性也从第6天直到第8天下降并保持低于M507菌株。在培养结束时 的低蛋白酶活性解释了M667菌株为什么产生与M507菌株相比两倍多的抗 体。
表22.1：用于产生沉默载体的引物
表22.2：RNAi载体中的靶序列
表22.3：用于筛选pTTv115/Δslp2-pyr4盒整合和菌株纯度的引物
(74)用于筛选pTTv115(Δslp2-pyr4)的整合
用于筛选slp2(tre123244)ORF的删除
表22.4：RNAi菌株和对照菌株的发酵培养物的总MAB01抗体表达水平。蛋 白G纯化的免疫球蛋白。
表22.5：摇瓶培养菌丝体的干重测量。对于各转化体和菌株生长两个瓶。菌 株表达针对降低slp2表达的RNAi。
表22.6：包含RNAi构建体的菌株中slp2表达的下调
表22.7：具有RNAi构建体的菌株中slp2、slp3、slp5和slp6表达的下调
表22.8：FTR104培养在TrMM加20g/L酵母提取物、40g/L纤维素、80g/L 纤维二糖和40g/L山梨糖，pH 5.5中生长。48小时后温度从28℃切换到 22℃。总抗体滴度以g/L表示。
天 M507 M646 M665 M666 M667
总Ab g/L g/L g/L g/L g/L
3 1.4 0.7 1.5 1.5 1.5
4 2.6 1.3 2.6 2.8 2.8
5 3.1 1.8 3.4 3.5 3.6
6 2.2 2.0 2.7 2.8 3.8
表22.9：具有RNAi的菌株和对照菌株的发酵上清液的蛋白酶活性测量。pH 5.5下对于酪蛋白底物的总蛋白酶活性。
实施例23-抗体片段表达里氏木霉菌株的产生
产生表达抗体片段(Fab、多聚单域抗体(sd-Ab's)和scFV)的七个里氏木霉 菌株形成表23中所列的不同蛋白酶删除背景。用于这一目的的遗传表达盒的 结构是基于纤维二糖水解酶I(cbh1)基因的调控元件(启动子和终止子)。 CBHI蛋白的催化结构域经修饰以除去内含子序列并用作融合伴体以增强抗 体片段表达和分泌。Kex2蛋白酶的识别基序插入融合伴体之间以促进抗体片 段从CBHI载体蛋白的共分泌释放。表达盒侧邻同源区域以允许靶向整合到 里氏木霉cbh1基因座。整个构建体保存在克隆载体中。
为了制备用于转化的侧翼表达盒，相应的片段通过PmeI限制性消化从其 相应载体骨架释放并使用illustra GFX PCR DNA和Gel Band Purification Kit (GEHealthcare)纯化。
如表23中所列的，里氏木霉蛋白酶删除菌株利用PEG-介导的原生质体 转化用纯化的表达盒转化。转化体通过将它们接种到分别包含潮霉素B作为 选择剂或乙酰胺作为单独氮源的培养基上针对潮霉素B抗性或乙酰胺酶原养 型进行选择。最多48个转化体使用构建体5'侧翼区域片段外的正向引物和修 饰的CBHI催化结构域内的反向引物(5'整合)以及分别潮霉素B或乙酰胺酶选 择标记内的正向引物，和3'侧翼区域外的反向引物(3'整合)各自通过PCR筛 选表达盒对cbh1基因座的同源整合。对于各转化，五至七个独立转化体(PCR筛选证明构建体正确整合到cbh1基因座)选择用于单孢子纯化以获得单核克 隆。破坏盒的适当整合使用如上所述的相同引物组合通过PCR再次证实且亲 本CBHI基因座的缺乏通过使用靶向cbh1开放阅读框的引物组合证实。破坏 盒的正确整合另外地使用DNA印迹杂交对于所有克隆进行验证。单核克隆 以及亲本菌株的基因组DNA独立地用两种不同的限制性酶组合消化并针对 cbh1基因的5'和3'侧翼探测以确认预期的cbh1基因座的修饰。
抗体片段(Fab、单域抗体和scFV)的表达和滴度分析
抗体片段的表达通过纤维二糖水解酶I启动子促进。菌株在包含2％酵母 提取物、4％纤维素、8％纤维二糖、4％山梨糖、5g/L KH2PO4和5g/L(NH4)2SO4的培养基中在分批发酵中生长7天。培养物pH控制在pH 5.5(用NH4OH调 节)和温度恒定保持在28℃。发酵在具有1L培养体积的4个平行的2L玻 璃容器反应器(DASGIP)中进行。在操作过程中获取培养上清液样品并储存在 -20℃下。样品在这些培养的整个过程中每日收集，且产生水平对于所有分子通过亲和液相色谱进行分析。对于各抗体片段，最大滴度、菌株ID和蛋白 酶删除背景表示在表23中。
表23：各种抗体片段的表达水平
*提供的百分比是近似的(～)或未测定(nd)
滴度测定
Mab和sdAb
Mab和sdAb浓度通过HPLC–蛋白A色谱定量，其基于具有UV检测 的亲和色谱。G类别的人免疫球蛋白(亚型类别：IgG1、IgG2、IgG4，除了IgG3) 的Fc-结构域特异性结合蛋白A(其与固定相共价连接)。蛋白A与Fc-结构 域的结合亲和力是pH依赖性的。在pH 7.5下结合后，单克隆抗体在pH 2.0 的酸性条件下洗脱并在280nm处检测。
Fab
Fab浓度通过HPLC–抗λ色谱定量，其基于具有UV检测的亲和色谱。 人Fab片段的λ链特异性结合camelid源的抗λ配体(其与固定相共价连接)。 在pH 7.5下结合后，单克隆抗体在pH 1.4的酸性条件下洗脱并在280nm处 检测。
scFV2
scFV2浓度使用avant系统和随后的UV检测通过HisTrap Protein L纯化定量。scFV2的κ轻链部分特异性地结合与固定相共价连接的Protein L 配体。在pH 7.2下结合后，scFV2用0.1mM HC1在酸性条件下洗脱并在280 nm处检测。
scFV1-His
scFV1-His浓度使用avant系统和随后的UV检测通过HisTrap HP纯化定量。蛋白质的组氨酸标签特异性地结合Ni琼脂糖。在结合后，蛋 白酶使用500mM Imidazol洗脱并在280nm处检测。
序列表
<110> LANDOWSKI, Christopher
HUUSKONEN, Anne
SAARINEN, Juhani
WESTERHOLM-PARVINEN, Ann
KANERVA, Anne
NATUNEN, Jari
HANNINEN, Anna-Liisa
SALOVUORI, Noora
PENTTILA, Merja
SALOHEIMO, Markku
<120> 蛋白酶缺陷丝状真菌细胞及其使用方法
<130> 61967-3001900
<160> 749
<170> PatentIn version 3.5
<210> 1
<211> 407
<212> PRT
<213> 里氏木霉
<400> 1
Met Gln Thr Phe Gly Ala Phe Leu Val Ser Phe Leu Ala Ala Ser Gly
1 5 10 15
Leu Ala Ala Ala Leu Pro Thr Glu Gly Gln Lys Thr Ala Ser Val Glu
20 25 30
Val Gln Tyr Asn Lys Asn Tyr Val Pro His Gly Pro Thr Ala Leu Phe
35 40 45
Lys Ala Lys Arg Lys Tyr Gly Ala Pro Ile Ser Asp Asn Leu Lys Ser
50 55 60
Leu Val Ala Ala Arg Gln Ala Lys Gln Ala Leu Ala Lys Arg Gln Thr
65 70 75 80
Gly Ser Ala Pro Asn His Pro Ser Asp Ser Ala Asp Ser Glu Tyr Ile
85 90 95
Thr Ser Val Ser Ile Gly Thr Pro Ala Gln Val Leu Pro Leu Asp Phe
100 105 110
Asp Thr Gly Ser Ser Asp Leu Trp Val Phe Ser Ser Glu Thr Pro Lys
115 120 125
Ser Ser Ala Thr Gly His Ala Ile Tyr Thr Pro Ser Lys Ser Ser Thr
130 135 140
Ser Lys Lys Val Ser Gly Ala Ser Trp Ser Ile Ser Tyr Gly Asp Gly
145 150 155 160
Ser Ser Ser Ser Gly Asp Val Tyr Thr Asp Lys Val Thr Ile Gly Gly
165 170 175
Phe Ser Val Asn Thr Gln Gly Val Glu Ser Ala Thr Arg Val Ser Thr
180 185 190
Glu Phe Val Gln Asp Thr Val Ile Ser Gly Leu Val Gly Leu Ala Phe
195 200 205
Asp Ser Gly Asn Gln Val Arg Pro His Pro Gln Lys Thr Trp Phe Ser
210 215 220
Asn Ala Ala Ser Ser Leu Ala Glu Pro Leu Phe Thr Ala Asp Leu Arg
225 230 235 240
His Gly Gln Asn Gly Ser Tyr Asn Phe Gly Tyr Ile Asp Thr Ser Val
245 250 255
Ala Lys Gly Pro Val Ala Tyr Thr Pro Val Asp Asn Ser Gln Gly Phe
260 265 270
Trp Glu Phe Thr Ala Ser Gly Tyr Ser Val Gly Gly Gly Lys Leu Asn
275 280 285
Arg Asn Ser Ile Asp Gly Ile Ala Asp Thr Gly Thr Thr Leu Leu Leu
290 295 300
Leu Asp Asp Asn Val Val Asp Ala Tyr Tyr Ala Asn Val Gln Ser Ala
305 310 315 320
Gln Tyr Asp Asn Gln Gln Glu Gly Val Val Phe Asp Cys Asp Glu Asp
325 330 335
Leu Pro Ser Phe Ser Phe Gly Val Gly Ser Ser Thr Ile Thr Ile Pro
340 345 350
Gly Asp Leu Leu Asn Leu Thr Pro Leu Glu Glu Gly Ser Ser Thr Cys
355 360 365
Phe Gly Gly Leu Gln Ser Ser Ser Gly Ile Gly Ile Asn Ile Phe Gly
370 375 380
Asp Val Ala Leu Lys Ala Ala Leu Val Val Phe Asp Leu Gly Asn Glu
385 390 395 400
Arg Leu Gly Trp Ala Gln Lys
405
<210> 2
<211> 404
<212> PRT
<213> 有性哈茨木霉
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Met Gln Thr Phe Gly Ala Phe Leu Val Ser Phe Leu Ala Ala Ser Gly
1 5 10 15
Met Ala Ala Ala Leu Pro Thr Glu Gly Gln Lys Thr Ala Ser Ile Glu
20 25 30
Val Ala Tyr Asn Lys Asn Tyr Val Ala His Gly Pro Thr Ala Leu Phe
35 40 45
Lys Ala Lys Arg Lys Tyr Gly Ala Pro Ile Ser Asp Asn Leu Arg Ala
50 55 60
Ala Val Ala Ala Lys His Ser Leu Thr Lys Arg Gln Thr Gly Ser Ala
65 70 75 80
Asn Thr Asn Pro Ser Asp Ser Ala Asp Asp Glu Tyr Ile Thr Ser Val
85 90 95
Ser Ile Gly Thr Pro Ala Gln Val Leu Pro Leu Asp Phe Asp Thr Gly
100 105 110
Ser Ser Asp Leu Trp Val Phe Ser Ser Glu Thr Pro Lys Ser Ser Ala
115 120 125
Ser Gly His Ala Leu Tyr Ser Pro Ser Lys Ser Ser Thr Ala Lys Lys
130 135 140
Leu Ser Gly Ser Thr Trp Ser Ile Glu Tyr Gly Asp Lys Ser Ser Ser
145 150 155 160
Ser Gly Asp Val Tyr Thr Asp Ile Val Thr Ile Gly Gly Phe Ser Val
165 170 175
Lys Thr Gln Ala Val Glu Ser Ala Thr Lys Val Ser Thr Gln Phe Val
180 185 190
Gln Asp Thr Val Ile Ser Gly Leu Val Gly Leu Gly Phe Asp Ile Gly
195 200 205
Asn Gln Val Ser Pro Arg Pro Gln Lys Thr Trp Phe Ser Asn Ala Ala
210 215 220
Ser Ser Leu Ala Glu Pro Leu Phe Thr Ala Asp Leu Arg His Gln Glu
225 230 235 240
Thr Gly Ser Tyr Asn Phe Gly Phe Ile Asp Thr Ser Leu Ala Lys Gly
245 250 255
Thr Ile Gly Tyr Thr Pro Ala Asp Gly Ser Glu Gly Tyr Trp Gly Phe
260 265 270
Thr Ala Thr Gly Tyr Ser Val Gly Gly Ala Lys Leu Asn Arg Asn Ser
275 280 285
Ile Thr Gly Ile Ala Asp Thr Gly Thr Thr Leu Leu Leu Leu Pro Asp
290 295 300
Asn Ile Val Asp Ala Tyr Tyr Asn Asn Val Glu Ser Ala Gln Tyr Asp
305 310 315 320
Asp Ser Gln Glu Gly Val Val Phe Asp Cys Ser Glu Asp Leu Pro Ser
325 330 335
Phe Ser Phe Gly Val Gly Gly Gln Thr Ile Thr Ile Ser Gly Asp Leu
340 345 350
Leu Asn Leu Thr Pro Ile Glu Glu Gly Ser Ser Thr Cys Phe Gly Gly
355 360 365
Leu Gln Ser Ser Ala Asp Ile Gly Ile Asn Ile Phe Gly Asp Val Ala
370 375 380
Leu Lys Ala Ala Leu Val Val Phe Asp Leu Gly Asn Glu Arg Leu Gly
385 390 395 400
Trp Ala Gln Lys
<210> 3
<211> 405
<212> PRT
<213> 棘孢木霉
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Met Gln Thr Phe Gly Ala Phe Leu Val Ser Phe Leu Ala Ala Ser Gly
1 5 10 15
Met Ala Ala Ala Leu Pro Ala Glu Gly Gln Gln Lys Thr Ile Ser Val
20 25 30
Pro Val Ile Tyr Asn Ala Asn His Ala Pro His Gly Pro Ser Ala Leu
35 40 45
Tyr Lys Ala Lys Lys Lys Phe Gly Ala Pro Ile Ser Glu Ser Leu Lys
50 55 60
Asn Asn Val Ala Gln His Lys Ala Ala Lys Leu Ala Arg Arg Gln Thr
65 70 75 80
Gly Ser Ala Pro Asn His Pro Ser Asp Ser Glu Asp Asp Glu Tyr Ile
85 90 95
Thr Asn Val Ser Ile Gly Thr Pro Ala Gln Val Leu Pro Leu Asp Phe
100 105 110
Asp Thr Gly Ser Ser Asp Leu Trp Val Phe Ser Ser Glu Thr Pro Ser
115 120 125
Ser Gln Ala Lys Gly His Thr Leu Tyr Asn Pro Thr Lys Ser Ser Thr
130 135 140
Ser Lys Lys Leu Ser Gly Tyr Ser Trp Thr Ile Ser Tyr Gly Asp Gly
145 150 155 160
Ser Ser Ser Ser Gly Asp Val Tyr Thr Asp Thr Val Ser Val Gly Gly
165 170 175
Phe Ser Val Thr Gly Gln Ala Val Glu Ser Ala Thr Lys Val Ser Ser
180 185 190
Glu Phe Val Ser Asp Thr Ser Asn Ser Gly Leu Leu Gly Leu Ala Leu
195 200 205
Asp Ser Ile Asn Thr Val Ser Pro Lys Gln Gln Lys Thr Trp Phe Ser
210 215 220
Asn Ala Ser Ser Lys Leu Ala Gln Pro Val Phe Thr Ala Asp Leu Asn
225 230 235 240
His Gln Ala Ser Gly Ser Tyr Asn Phe Gly Tyr Ile Asp Thr Ser Leu
245 250 255
Ala Ser Gly Pro Ile Ser Tyr Val Pro Ile Ser Thr Ala Asn Gly Phe
260 265 270
Trp Glu Phe Thr Ser Ala Ser Tyr Ala Ile Gly Ser Gly Ser Thr Lys
275 280 285
Lys His Ser Thr Asp Gly Ile Ala Asp Thr Gly Thr Thr Leu Leu Leu
290 295 300
Leu Asp Asp Thr Ile Val Asp Ala His Tyr Gly Gln Val Ser Ser Ala
305 310 315 320
Gln Tyr Asp Asn Ser Gln Glu Gly Tyr Thr Phe Asp Cys Asp Glu Asn
325 330 335
Leu Pro Ser Phe Thr Phe Ala Val Gly Ser Ser Lys Ile Thr Val Pro
340 345 350
Gly Ser Leu Ile Asn Phe Ala Pro Val Ser Gly Asn Thr Cys Phe Gly
355 360 365
Gly Leu Gln Ser Asn Asp Gly Ile Gly Ile Asn Ile Phe Gly Asp Val
370 375 380
Ala Ile Lys Ala Ala Leu Val Val Phe Asp Leu Gly Asn Lys Arg Leu
385 390 395 400
Gly Trp Ala Gln Lys
405
<210> 4
<211> 405
<212> PRT
<213> 深绿木霉
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Met Gln Thr Phe Gly Ala Phe Leu Val Ser Phe Leu Ala Ala Ser Gly
1 5 10 15
Leu Ala Ala Ala Leu Pro Ala Glu Gly Gln Gln Lys Thr Ile Ser Val
20 25 30
Pro Val Lys Tyr Asn Ala Asn His Ala Pro His Gly Pro Thr Glu Leu
35 40 45
Phe Lys Ala Lys Arg Lys Phe Gly Ala Pro Ile Ser Glu Asn Leu Lys
50 55 60
Asn Arg Val Ala Glu His Lys Ala Ala Lys Leu Ala Arg Arg Gln Thr
65 70 75 80
Gly Ser Ala Pro Asn His Pro Ser Asp Ser Glu Asp Asp Glu Tyr Ile
85 90 95
Thr Asn Val Ser Ile Gly Thr Pro Ala Gln Val Leu Pro Leu Asp Phe
100 105 110
Asp Thr Gly Ser Ser Asp Leu Trp Val Phe Ser Ser Glu Thr Pro Ser
115 120 125
Ser Ser Ala Arg Gly His Thr Ile Tyr Thr Pro Ser Lys Ser Ser Thr
130 135 140
Ser Lys Lys Val Ser Gly Ala Ser Trp Ser Ile Thr Tyr Gly Asp Asn
145 150 155 160
Ser Ser Ser Ser Gly Asp Val Tyr Thr Asp Thr Val Thr Ile Gly Gly
165 170 175
Phe Ser Val Thr Gly Gln Ala Val Glu Ser Ala Thr Lys Val Ser Thr
180 185 190
Gln Phe Val Gln Asp Thr Ser Asn Ser Gly Leu Val Gly Leu Ala Leu
195 200 205
Asp Ser Gly Asn Thr Val Lys Pro Thr Lys Gln Lys Thr Trp Phe Ser
210 215 220
Asn Ala Ser Ser His Leu Ala Gln Pro Val Phe Thr Ala Asp Leu Asn
225 230 235 240
His Gln Ala Asp Gly Ser Tyr Asn Phe Gly Phe Ile Asp Thr Thr Leu
245 250 255
Ala Ser Gly Pro Ile Thr Tyr Val Pro Ile Ser Thr Gly Asn Gly Phe
260 265 270
Trp Glu Phe Thr Ser Ala Ser Tyr Ala Ile Gly Gly Gly Ser Ser Lys
275 280 285
Lys His Ser Thr Asp Gly Ile Ala Asp Thr Gly Thr Thr Leu Leu Leu
290 295 300
Leu Asp Asp Thr Ile Val Asp Ala Tyr Tyr Asn Gln Val Glu Ser Ala
305 310 315 320
Gln Tyr Asp Asp Ser Gln Glu Gly Tyr Thr Phe Asp Cys Ser Glu Asp
325 330 335
Leu Pro Ser Phe Ser Phe Ala Val Gly Ser Ser Thr Ile Thr Val Pro
340 345 350
Gly Ser Leu Ile Asn Phe Ala Pro Val Asp Gly Asn Thr Cys Phe Gly
355 360 365
Gly Leu Gln Ser Asp Asp Gly Ile Gly Ile Ser Ile Phe Gly Asp Val
370 375 380
Ala Ile Lys Ala Ala Leu Val Val Phe Asp Leu Gly Asn Lys Arg Leu
385 390 395 400
Gly Trp Ala Gln Lys
405
<210> 5
<211> 404
<212> PRT
<213> 绿木霉
<400> 5
Met Gln Thr Phe Gly Ala Phe Leu Val Ser Phe Leu Ala Ala Ser Gly
1 5 10 15
Leu Ala Ala Ala Leu Pro Ala Glu Gly Gln Lys Thr Ala Ser Ile Glu
20 25 30
Val Ser Tyr Asn Lys His Tyr Val Pro His Gly Pro Thr Ala Leu Phe
35 40 45
Lys Ala Lys Arg Lys Tyr Asn Ala Pro Ile Ser Glu Lys Leu His Ala
50 55 60
Ala Val Ala Ala Lys Lys Ala Leu Thr Lys Arg Gln Thr Gly Ser Ala
65 70 75 80
Asn Thr Asn Pro Ser Asp Ser Ala Asp Asp Glu Tyr Ile Thr Asn Val
85 90 95
Ser Ile Gly Thr Pro Ala Gln Val Leu Pro Leu Asp Phe Asp Thr Gly
100 105 110
Ser Ser Asp Leu Trp Val Phe Ser Ser Glu Thr Pro Lys Ser Ser Ala
115 120 125
Ser Gly His Val Thr Tyr Ser Pro Ser Lys Ser Ser Thr Ala Lys Lys
130 135 140
Leu Ser Gly Ala Ser Trp Ser Ile Thr Tyr Gly Asp His Ser Ser Ser
145 150 155 160
Ser Gly Asp Val Tyr Thr Asp Lys Val Thr Val Gly Gly Phe Ser Val
165 170 175
Ser Ser Gln Ala Val Glu Ser Ala Thr Lys Val Ser Thr Gln Phe Val
180 185 190
Gln Asp Thr Val Ile Ser Gly Leu Leu Gly Leu Gly Phe Asp Val Gly
195 200 205
Asn Thr Val Lys Pro Arg Ala Gln Lys Thr Trp Phe Ser Asn Ala Ala
210 215 220
Asn Ser Leu Ala Gln Pro Leu Phe Thr Ala Asp Leu Lys His Gln Gln
225 230 235 240
Thr Gly Ser Tyr Asn Phe Gly Phe Ile Asp Thr Thr Leu Ala Lys Gly
245 250 255
Pro Ile Gly Tyr Thr Pro Ala Asp Gly Ser Glu Gly Tyr Trp Gly Phe
260 265 270
Thr Ala Thr Gly Tyr Ser Val Gly Gly Ala Lys Leu Asn Arg Asn Ser
275 280 285
Ile Thr Gly Ile Ala Asp Thr Gly Thr Thr Leu Leu Leu Leu Asp Asp
290 295 300
Asp Val Val Asn Ala Tyr Tyr Asn Asn Val Glu Ser Ala Gln Tyr Asp
305 310 315 320
Asp Ser Gln Gln Gly Val Val Phe Asp Cys Ser Glu Asp Leu Pro Ser
325 330 335
Phe Ser Phe Gly Val Gly Gly Thr Thr Ile Thr Ile Pro Gly Ser Leu
340 345 350
Ile Asn Leu Thr Pro Val Ser Asp Gly Ser Ser Thr Cys Phe Gly Gly
355 360 365
Leu Gln Ser Ser Ala Asp Ile Gly Ile Asn Ile Phe Gly Asp Val Ala
370 375 380
Leu Lys Ala Ala Leu Val Val Phe Asp Leu Gly Asn Glu Arg Leu Gly
385 390 395 400
Trp Ala Gln Lys
<210> 6
<211> 395
<212> PRT
<213> 烟曲霉
<400> 6
Met Val Val Phe Ser Lys Val Thr Ala Val Val Val Gly Leu Ser Thr
1 5 10 15
Ile Val Ser Ala Val Pro Val Val Gln Pro Arg Lys Gly Phe Thr Ile
20 25 30
Asn Gln Val Ala Arg Pro Val Thr Asn Lys Lys Thr Val Asn Leu Pro
35 40 45
Ala Val Tyr Ala Asn Ala Leu Thr Lys Tyr Gly Gly Thr Val Pro Asp
50 55 60
Ser Val Lys Ala Ala Ala Ser Ser Gly Ser Ala Val Thr Thr Pro Glu
65 70 75 80
Gln Tyr Asp Ser Glu Tyr Leu Thr Pro Val Lys Val Gly Gly Thr Thr
85 90 95
Leu Asn Leu Asp Phe Asp Thr Gly Ser Ala Asp Leu Trp Val Phe Ser
100 105 110
Ser Glu Leu Ser Ala Ser Gln Ser Ser Gly His Ala Ile Tyr Lys Pro
115 120 125
Ser Ala Asn Ala Gln Lys Leu Asn Gly Tyr Thr Trp Lys Ile Gln Tyr
130 135 140
Gly Asp Gly Ser Ser Ala Ser Gly Asp Val Tyr Lys Asp Thr Val Thr
145 150 155 160
Val Gly Gly Val Thr Ala Gln Ser Gln Ala Val Glu Ala Ala Ser His
165 170 175
Ile Ser Ser Gln Phe Val Gln Asp Lys Asp Asn Asp Gly Leu Leu Gly
180 185 190
Leu Ala Phe Ser Ser Ile Asn Thr Val Ser Pro Arg Pro Gln Thr Thr
195 200 205
Phe Phe Asp Thr Val Lys Ser Gln Leu Asp Ser Pro Leu Phe Ala Val
210 215 220
Thr Leu Lys Tyr His Ala Pro Gly Thr Tyr Asp Phe Gly Tyr Ile Asp
225 230 235 240
Asn Ser Lys Phe Gln Gly Glu Leu Thr Tyr Thr Asp Val Asp Ser Ser
245 250 255
Gln Gly Phe Trp Met Phe Thr Ala Asp Gly Tyr Gly Val Gly Asn Gly
260 265 270
Ala Pro Asn Ser Asn Ser Ile Ser Gly Ile Ala Asp Thr Gly Thr Thr
275 280 285
Leu Leu Leu Leu Asp Asp Ser Val Val Ala Asp Tyr Tyr Arg Gln Val
290 295 300
Ser Gly Ala Lys Asn Ser Asn Gln Tyr Gly Gly Tyr Val Phe Pro Cys
305 310 315 320
Ser Thr Lys Leu Pro Ser Phe Thr Thr Val Ile Gly Gly Tyr Asn Ala
325 330 335
Val Val Pro Gly Glu Tyr Ile Asn Tyr Ala Pro Val Thr Asp Gly Ser
340 345 350
Ser Thr Cys Tyr Gly Gly Ile Gln Ser Asn Ser Gly Leu Gly Phe Ser
355 360 365
Ile Phe Gly Asp Ile Phe Leu Lys Ser Gln Tyr Val Val Phe Asp Ser
370 375 380
Gln Gly Pro Arg Leu Gly Phe Ala Pro Gln Ala
385 390 395
<210> 7
<211> 404
<212> PRT
<213> 米曲霉
<400> 7
Met Val Ile Leu Ser Lys Val Ala Ala Val Ala Val Gly Leu Ser Thr
1 5 10 15
Val Ala Ser Ala Leu Pro Thr Gly Pro Ser His Ser Pro His Ala Arg
20 25 30
Arg Gly Phe Thr Ile Asn Gln Ile Thr Arg Gln Thr Ala Arg Val Gly
35 40 45
Pro Lys Thr Ala Ser Phe Pro Ala Ile Tyr Ser Arg Ala Leu Ala Lys
50 55 60
Tyr Gly Gly Thr Val Pro Ala His Leu Lys Ser Ala Val Ala Ser Gly
65 70 75 80
His Gly Thr Val Val Thr Ser Pro Glu Pro Asn Asp Ile Glu Tyr Leu
85 90 95
Thr Pro Val Asn Ile Gly Gly Thr Thr Leu Asn Leu Asp Phe Asp Thr
100 105 110
Gly Ser Ala Asp Leu Trp Val Phe Ser Glu Glu Leu Pro Lys Ser Glu
115 120 125
Gln Thr Gly His Asp Val Tyr Lys Pro Ser Gly Asn Ala Ser Lys Ile
130 135 140
Ala Gly Ala Ser Trp Asp Ile Ser Tyr Gly Asp Gly Ser Ser Ala Ser
145 150 155 160
Gly Asp Val Tyr Gln Asp Thr Val Thr Val Gly Gly Val Thr Ala Gln
165 170 175
Gly Gln Ala Val Glu Ala Ala Ser Lys Ile Ser Asp Gln Phe Val Gln
180 185 190
Asp Lys Asn Asn Asp Gly Leu Leu Gly Leu Ala Phe Ser Ser Ile Asn
195 200 205
Thr Val Lys Pro Lys Pro Gln Thr Thr Phe Phe Asp Thr Val Lys Asp
210 215 220
Gln Leu Asp Ala Pro Leu Phe Ala Val Thr Leu Lys Tyr His Ala Pro
225 230 235 240
Gly Ser Tyr Asp Phe Gly Phe Ile Asp Lys Ser Lys Phe Thr Gly Glu
245 250 255
Leu Ala Tyr Ala Asp Val Asp Asp Ser Gln Gly Phe Trp Gln Phe Thr
260 265 270
Ala Asp Gly Tyr Ser Val Gly Lys Gly Asp Ala Gln Lys Ala Pro Ile
275 280 285
Thr Gly Ile Ala Asp Thr Gly Thr Thr Leu Val Met Leu Asp Asp Glu
290 295 300
Ile Val Asp Ala Tyr Tyr Lys Gln Val Gln Gly Ala Lys Asn Asp Ala
305 310 315 320
Ser Ala Gly Gly Tyr Val Phe Pro Cys Glu Thr Glu Leu Pro Glu Phe
325 330 335
Thr Val Val Ile Gly Ser Tyr Asn Ala Val Ile Pro Gly Lys His Ile
340 345 350
Asn Tyr Ala Pro Leu Gln Glu Gly Ser Ser Thr Cys Val Gly Gly Ile
355 360 365
Gln Ser Asn Ser Gly Leu Gly Leu Ser Ile Leu Gly Asp Val Phe Leu
370 375 380
Lys Ser Gln Tyr Val Val Phe Asp Ser Gln Gly Pro Arg Leu Gly Phe
385 390 395 400
Ala Ala Gln Ala
<210> 8
<211> 407
<212> PRT
<213> 金龟子绿僵菌
<400> 8
Met Gln Thr Phe Gly Ser Phe Leu Val Ser Leu Val Val Ala Ser Ser
1 5 10 15
Leu Ala Thr Ala Leu Pro Thr Thr Pro Ser Ser Thr Pro Ala Gly Asn
20 25 30
Val Ser Val Thr Ala Lys His Asn Ala Asn Phe Thr Arg Asn Gly Pro
35 40 45
Ala Ala Leu Ala Lys Ala Tyr Arg Lys Phe Gly Lys Pro Val Pro Val
50 55 60
Asp Val Ala Asn Ala Leu Ser Arg Gln Asn Ser Lys Arg Thr Thr Gly
65 70 75 80
Ser Asp Pro Asn Ser Pro Gln Gln Tyr Asp Ile Glu Tyr Leu Ala Pro
85 90 95
Val Gln Ile Gly Thr Pro Ala Gln Thr Leu Asn Leu Asp Phe Asp Thr
100 105 110
Gly Ser Ser Asp Leu Trp Val Phe Ser Ser Leu Thr Pro Ser Ser Gln
115 120 125
Val Asn Gly Gln Thr Leu Tyr Asn Pro Ser Lys Ser Ser Thr Ala Gln
130 135 140
Ser Leu Ser Gly Ser Thr Trp Ser Ile Thr Tyr Gly Asp Gly Ser Ser
145 150 155 160
Ser Ser Gly Val Val Tyr Thr Asp Ala Val Thr Val Gly Gly Leu Thr
165 170 175
Val Asn Ala Gln Ala Val Glu Ala Ala Lys Gln Val Ser Ser Gln Phe
180 185 190
Ser Gln Asp Ala Ala Ser Ser Gly Leu Leu Gly Leu Ala Phe Ser Ser
195 200 205
Ile Asn Thr Val Lys Pro Thr Lys Gln Lys Thr Phe Phe Asp Asn Ala
210 215 220
Lys Pro Ala Leu Asp Ser Ala Leu Phe Thr Ala Asp Leu Lys His Gly
225 230 235 240
Ala Asp Gly Lys Tyr Asn Phe Gly Tyr Ile Asp Ser Thr Ala Tyr Thr
245 250 255
Gly Ser Ile Ala Tyr Thr Pro Val Asp Ser Ser Gln Gly Phe Trp Gly
260 265 270
Phe Thr Ser Arg Gly Tyr Ala Val Gly Gly Gly Ser Leu Asn Thr Ala
275 280 285
Ser Thr Ser Gly Ile Ala Asp Thr Gly Thr Thr Leu Leu Leu Leu Pro
290 295 300
Ser Ser Ile Val Ser Ala Tyr Tyr Ala Lys Val Ser Gly Ala Arg Tyr
305 310 315 320
Asp Ser Ser Gln Gly Gly Tyr Thr Phe Ala Cys Ser Gln Ser Leu Pro
325 330 335
Ser Phe Ser Phe Gly Val Ser Gly Ala Thr Val Thr Val Pro Gly Ala
340 345 350
Tyr Leu Asn Tyr Ala Pro Thr Asp Ser Thr Gly Arg Thr Cys Phe Gly
355 360 365
Gly Leu Gln Ser Ser Ser Gly Ile Gly Ile Asn Ile Phe Gly Asp Val
370 375 380
Ala Leu Lys Ser Ala Phe Val Val Phe Asp Gly Gly Asn Asn Arg Leu
385 390 395 400
Gly Trp Ala Ala Lys Ala Leu
405
<210> 9
<211> 406
<212> PRT
<213> 玉米赤霉
<400> 9
Met Pro Ser Leu Asn Ala Leu Leu Ser Ala Ser Leu Ala Phe Ala Ser
1 5 10 15
Ile Ala Leu Gly Ala Pro Ala Val Gln Asp Lys His Phe Ser Val Glu
20 25 30
Gln Val Lys Asn Pro Gln Phe Val Lys Asn Gly Pro Leu Ala Leu Ala
35 40 45
His Val Tyr Ala Lys Tyr Gly Val Pro Leu Pro Lys Gly Leu Glu Lys
50 55 60
Ala Val Gln Lys Val Lys Ser Ser His Thr Lys Arg Gln Asn Gly Ser
65 70 75 80
Gly Ser Val Ile Thr Thr Pro Gln Asp Glu Asp Ile Glu Trp Leu Thr
85 90 95
Pro Val Gln Ile Gly Thr Pro Ala Gln Thr Leu Asn Leu Asp Phe Asp
100 105 110
Thr Gly Ser Ser Asp Leu Trp Val Phe Ser Thr Glu Thr Ser Gly Ser
115 120 125
Ser Gly His Asp Glu Tyr Asn Pro Ala Lys Ser Ser Thr Ser Lys Lys
130 135 140
Leu Ser Gly Ala Thr Trp Ser Ile Ser Tyr Gly Asp Gly Ser Thr Ser
145 150 155 160
Ser Gly Asp Val Tyr Lys Asp Lys Val Ser Val Gly Gly Leu Val Val
165 170 175
Ser Ser Gln Ala Val Glu Ser Ala Gln Arg Val Ser Asp Gln Phe Glu
180 185 190
Gln Glu Thr Gly Leu Asp Gly Leu Leu Gly Leu Gly Phe Ser Ser Ile
195 200 205
Asn Thr Val Thr Pro Thr Gln Gln Asn Thr Phe Phe Asp Asn Ala Ala
210 215 220
Pro Ser Leu Gln Ser Ala Val Phe Thr Ala Asn Leu Lys His Gln Lys
225 230 235 240
Pro Gly Thr Tyr Asn Phe Gly Phe Ile Asp Ser Ser Ser Tyr Thr Gly
245 250 255
Lys Ile Gly Tyr Ala Pro Val Asp Ser Ser Gln Gly Phe Trp Glu Phe
260 265 270
Thr Ala Ser Gly Tyr Ala Val Gly Ser Ala Thr Val Asn Arg Ser Pro
275 280 285
Ile Thr Gly Ile Ala Asp Thr Gly Thr Thr Leu Leu Leu Leu Pro Ser
290 295 300
Ala Ile Asn Ser Ala Tyr Tyr Ala Lys Val Ser Gly Ala Arg Tyr Ser
305 310 315 320
Ser Ser Tyr Gly Gly Tyr Val Phe Ser Cys Ser Ala Thr Leu Pro Asn
325 330 335
Phe Ser Phe Ala Val Gly Gly Val Thr Ile Thr Ile Pro Gly Ser Tyr
340 345 350
Ile Asn Tyr Ala Pro Val Gln Asp Gly Ser Ser Thr Cys Leu Gly Gly
355 360 365
Ile Gln Pro Ser Glu Asp Ile Gly Ile Asn Ile Phe Gly Asp Ile Ala
370 375 380
Leu Lys Ala Ala Phe Val Val Phe Asp Gly Gly Asn Gln Gln Val Gly
385 390 395 400
Trp Ala Lys Lys Thr Leu
405
<210> 10
<211> 407
<212> PRT
<213> Fusarium venenatum
<400> 10
Met Pro Ser Leu Asn Ala Leu Leu Ala Ala Ser Leu Ala Phe Ala Ser
1 5 10 15
Ile Ala Leu Gly Ala Pro Ala Val Gln Asp Lys His Phe Ser Val Glu
20 25 30
Gln Val Lys Asn Pro Lys Phe Ile Lys Ser Gly Pro Leu Ala Leu Ala
35 40 45
His Val Tyr Ser Lys Tyr Gly Val Pro Leu Pro Lys Gly Leu Glu Lys
50 55 60
Ala Val Lys Ala Val Lys Ser Ser Ser His Ser Lys Arg Gln Asn Gly
65 70 75 80
Ser Gly Ser Val Ile Thr Thr Pro Ala Asp Glu Asp Ile Glu Trp Leu
85 90 95
Thr Pro Val Gln Ile Gly Thr Pro Ala Gln Thr Leu Asn Leu Asp Phe
100 105 110
Asp Thr Gly Ser Ser Asp Leu Trp Val Phe Ser Thr Glu Thr Ser Gly
115 120 125
Ser Ser Gly His Asp Glu Tyr Asn Pro Ala Lys Ser Ser Thr Ser Lys
130 135 140
Lys Leu Ala Gly Ala Thr Trp Ser Ile Ser Tyr Gly Asp Gly Ser Thr
145 150 155 160
Ser Ser Gly Asp Val Tyr Lys Asp Lys Val Ser Val Gly Gly Leu Ala
165 170 175
Val Ala Ser Gln Ala Val Glu Ser Ala Gln Arg Val Ser Asp Glu Phe
180 185 190
Glu Gln Glu Thr Gly Leu Asp Gly Leu Leu Gly Leu Gly Phe Ser Ser
195 200 205
Ile Asn Thr Val Lys Pro Thr Lys Gln Lys Thr Phe Phe Asp Asn Ala
210 215 220
Ile Ser Thr Leu Gln Ser Pro Val Phe Ser Ala Asp Leu Lys His Gln
225 230 235 240
Gln Pro Gly Thr Tyr Asn Phe Gly Tyr Ile Asp Ser Ser Ala Tyr Thr
245 250 255
Gly Lys Ile Gly Tyr Ala Pro Ile Asp Ser Ser Gly Gly Trp Trp Glu
260 265 270
Phe Thr Ser Ser Gly Tyr Ala Val Gly Ser Ala Ala Leu Asn Lys Ser
275 280 285
Pro Ile Thr Gly Ile Ala Asp Thr Gly Thr Thr Leu Leu Leu Leu Pro
290 295 300
Asp Ala Val Asn Ser Ala Tyr Tyr Ala Lys Val Ser Gly Ala Arg Tyr
305 310 315 320
Ser Ser Ser Tyr Gly Gly Tyr Val Phe Ser Cys Ser Ala Thr Leu Pro
325 330 335
Asn Phe Ser Phe Ala Val Gly Gly Val Thr Ile Thr Ile Pro Gly Ser
340 345 350
Tyr Ile Asn Tyr Ala Pro Val Gln Gln Gly Ser Ser Thr Cys Leu Gly
355 360 365
Gly Ile Gln Pro Ser Asp Asp Ile Gly Ile Asn Ile Phe Gly Asp Ile
370 375 380
Ala Leu Lys Ala Ala Phe Val Val Phe Asp Gly Gly Asn Gln Gln Val
385 390 395 400
Gly Trp Ala Lys Lys Thr Leu
405
<210> 11
<211> 414
<212> PRT
<213> 尖孢镰刀菌
<400> 11
Met Pro Ser Ile Asn Ala Leu Leu Thr Ala Ser Leu Ala Phe Ala Ser
1 5 10 15
Ile Ala Leu Gly Ala Pro Ala Ala Gln Asp Lys Lys Phe Thr Val Glu
20 25 30
Gln Val Lys Asn Pro Lys Phe Ile Lys Asn Gly Pro Leu Ala Leu Ala
35 40 45
His Val Tyr Ala Lys Tyr Gly Val Pro Leu Pro Lys Gly Leu Glu Lys
50 55 60
Ala Val Lys Ala Val Arg Pro Pro His Ala His Ser Lys Arg Gln Ser
65 70 75 80
Gly Ser Gly Ser Ala Thr Thr Thr Pro Ser Asp Glu Asp Val Glu Trp
85 90 95
Leu Thr Pro Val Gln Ile Gly Thr Pro Ala Gln Thr Phe Asn Leu Asp
100 105 110
Phe Asp Thr Gly Ser Ser Asp Leu Trp Val Phe Ser Thr Glu Thr Thr
115 120 125
Gly Ser Ser Gly His Asp Glu Tyr Asn Pro Ala Lys Ser Ser Thr Ala
130 135 140
Lys Lys Leu Ser Gly Ala Thr Trp Lys Ile Ser Tyr Gly Asp Gly Ser
145 150 155 160
Ser Ser Ser Gly Asp Val Tyr Lys Asp Lys Val Ser Val Gly Gly Leu
165 170 175
Val Val Ser Ser Gln Ala Val Glu Ala Ala Gln Lys Val Ser Ser Glu
180 185 190
Phe Glu Gln Glu Thr Gly Leu Asp Gly Leu Leu Gly Leu Gly Phe Ser
195 200 205
Ser Ile Asn Thr Val Thr Pro Thr Gln Gln Lys Thr Phe Phe Asp Asn
210 215 220
Ala Ala Ser Thr Leu Gly Ser Pro Val Phe Thr Ala Asp Leu Lys His
225 230 235 240
Gln Lys Pro Gly Lys Tyr Asn Phe Gly Phe Ile Asp Ser Thr Ala Tyr
245 250 255
Thr Gly Lys Ile Gly Tyr Ala Ser Val Asp Ser Ser Glu Gly Phe Trp
260 265 270
Glu Phe Thr Ser Thr Gly Tyr Gly Ile Gly Ser Ala Ala Val Asn Lys
275 280 285
Ser Pro Ile Thr Gly Ile Ala Asp Thr Gly Thr Thr Leu Leu Leu Leu
290 295 300
Pro Asp Asp Val Asn Ser Ala Tyr Tyr Ala Lys Val Ser Gly Ala Arg
305 310 315 320
Tyr Ser Ser Ser Tyr Gly Gly Tyr Val Phe Ser Cys Ser Ala Thr Leu
325 330 335
Pro Ser Phe Ser Phe Val Val Gly Gly Val Thr Ile Thr Ile Pro Gly
340 345 350
Ser Tyr Ile Asn Tyr Ala Pro Val Gln Asp Gly Ser Thr Thr Cys Leu
355 360 365
Gly Gly Ile Gln Pro Ser Asp Asp Ile Gly Ile Asn Ile Phe Gly Asp
370 375 380
Ile Ala Leu Lys Ala Ala Phe Val Val Phe Asp Gly Gly Asn Gln Gln
385 390 395 400
Val Gly Trp Ala Lys Lys Thr Leu Leu Ser Leu Val Asp Ser
405 410
<210> 12
<211> 410
<212> PRT
<213> Grosmannia clavigera
<400> 12
Met Pro Ser Thr Thr Phe Leu Thr Val Ala Leu Ala Ala Val Ser Ala
1 5 10 15
Val Ser Ala Met Pro Ala Arg Ile Gly Val Pro Val Ser Thr Ser Asn
20 25 30
Phe Ser Ile His Gln Val Arg Asn Glu Gln Phe Thr Gly His His Gly
35 40 45
Pro Leu Ala Leu Ala Lys Ala Tyr Arg Lys Tyr Gly Val Ala Leu Pro
50 55 60
Thr Asp Leu Glu Ala Ala Val Ala Lys Ile Gln Ala Asp Gln Ala Val
65 70 75 80
Ser Arg Arg Asp Thr Gly Ser Ala Thr Asn Thr Pro Thr Ser Asp Asp
85 90 95
Ser Glu Tyr Leu Thr Pro Val Gln Ile Gly Thr Pro Ala Gln Thr Leu
100 105 110
His Leu Asp Phe Asp Thr Gly Ser Ser Asp Leu Trp Val Phe Ser Thr
115 120 125
Glu Thr Pro Ser Gly Ser Val Asp Gly Gln Thr Leu Tyr Lys Pro Ala
130 135 140
Ser Ser Ser Ser Ser Glu Lys Leu Thr Gly Ala Thr Trp Lys Ile Lys
145 150 155 160
Tyr Gly Asp Asp Ser Thr Ser Ser Gly Asp Val Tyr Thr Asp Val Val
165 170 175
Ser Val Gly Gly Leu Ser Val Gln Ser Gln Ala Val Glu Ser Ala Lys
180 185 190
Thr Val Ser Ser Ser Phe Thr Ser Asp Thr Ala Ser Ser Gly Leu Leu
195 200 205
Gly Leu Ala Phe Ser Ser Ile Asn Ala Val Leu Pro Asp Gln Gln Asn
210 215 220
Thr Phe Phe Asp Asn Ala Gln Asp Ser Leu Asp Ser Ala Leu Phe Thr
225 230 235 240
Val Asp Leu Lys His Gly Lys Ala Gly Lys Tyr Asn Phe Gly Tyr Ile
245 250 255
Asp Ser Ala Ala His Thr Gly Ser Ile Thr Tyr Thr Asp Val Asp Asn
260 265 270
Ala Lys Gly Phe Trp Gly Phe Thr Ala Asp Gly Tyr Lys Ile Gly Asn
275 280 285
Ala Ala Lys Lys Gly Thr Ser Ile Ser Gly Ile Ala Asp Thr Gly Thr
290 295 300
Thr Leu Leu Leu Leu Pro Asp Ala Val Val Asp Ala Tyr Tyr Ala Gly
305 310 315 320
Val Ser Gly Ala Ser Tyr Asp Ser Ser Gln Gly Gly Tyr Thr Phe Pro
325 330 335
Ser Ser Ala Thr Leu Pro Ala Phe Thr Tyr Tyr Val Glu Gly Val Ala
340 345 350
Phe Thr Val Pro Ala Ser Tyr Ile Lys Tyr Ala Ala Ile Asp Ser Ser
355 360 365
Thr Tyr Phe Gly Gly Ile Gln Ser Asp Ser Ser Ile Gly Phe Ser Ile
370 375 380
Phe Gly Asp Val Ala Leu Lys Ala Ala Phe Val Ile Phe Asp Ala Thr
385 390 395 400
Pro Lys Leu Gly Phe Ala Pro Lys Thr Leu
405 410
<210> 13
<211> 411
<212> PRT
<213> 黑白轮枝菌
<400> 13
Met Gln Thr Phe Gly Thr Phe Phe Val Ala Ile Leu Ala Ala Thr Gly
1 5 10 15
Tyr Ala Ala Val Val Pro Thr Lys Ile Gly Ile Pro Asp Gln Gly Thr
20 25 30
Phe Ser Val Arg Ala Val Pro Asn Pro Gly Phe Gln His Arg Asn Gly
35 40 45
Ala Ile Ala Leu Ala Lys Ala Tyr Asn Lys Phe Gly Lys Ala Ala Pro
50 55 60
Ala Asp Val Ala Ala Ala Ala Ala Asn Ala Thr Glu Ser Arg Leu Ser
65 70 75 80
Lys Arg Thr Thr Gly Ser Ala Thr Thr Ser Pro Glu Ala Asn Asp Val
85 90 95
Glu Tyr Leu Thr Pro Val Gln Ile Gly Thr Pro Ala Gln Asn Leu Val
100 105 110
Leu Asp Phe Asp Thr Gly Ser Ser Asp Leu Trp Val Phe Ser Thr Glu
115 120 125
Leu Pro Ser Ser Gln Val Asn Gly Gln Thr Arg Tyr Asn Pro Ala Ser
130 135 140
Ser Ser Thr Ser Ser Lys Val Ala Gly Ala Lys Trp Ser Ile Ser Tyr
145 150 155 160
Gly Asp Gly Ser Ser Ser Asn Gly Asp Val Tyr Leu Asp Lys Val Thr
165 170 175
Ile Gly Gly Leu Thr Val Lys Lys Gln Ala Val Glu Ala Ala Gln Lys
180 185 190
Val Ser Ser Ser Phe Thr Ser Asp Pro Leu Asp Gly Leu Leu Gly Leu
195 200 205
Ala Phe Ser Ser Ile Asn Thr Val Ser Pro Ser Arg Gln Leu Thr Phe
210 215 220
Phe Asp Asn Ala Lys Ser Thr Leu Asp Ala Pro Leu Phe Thr Ala Asp
225 230 235 240
Leu Lys His Ala Ala Asp Pro Ala Gly Lys Tyr Asn Phe Gly Tyr Ile
245 250 255
Asp Lys Thr Ala Tyr Lys Gly Ala Ile Thr Tyr Thr Asn Val Asp Asn
260 265 270
Ser Asp Gly Phe Trp Ser Phe Thr Ser Thr Gly Tyr Gln Val Gly Thr
275 280 285
Ala Ala Phe Lys Ser Ser Pro Val Thr Gly Ile Ala Asp Thr Gly Thr
290 295 300
Thr Leu Leu Leu Leu Pro Thr Ser Val Val Ser Ala Tyr Tyr Ala Gln
305 310 315 320
Val Ser Gly Ala Arg Tyr Asp Ser Ser Gln Gly Gly Tyr Val Phe Ser
325 330 335
Cys Ser Ala Thr Leu Pro Ala Phe Thr Phe Gly Val Glu Ser Ala Arg
340 345 350
Ile Lys Ile Pro Gly Ser Tyr Ile Asn Phe Ala Pro Val Asp Asp Ser
355 360 365
Gly Ser Val Cys Phe Gly Gly Ile Gln Arg Asn Thr Asn Ile Gly Phe
370 375 380
Ser Ile Phe Gly Asp Val Ala Leu Lys Ala Ala Phe Val Val Phe Asn
385 390 395 400
Gly Gly Asp Leu Thr Leu Gly Trp Ala Ser Lys
405 410
<210> 14
<211> 421
<212> PRT
<213> 球毛壳霉
<400> 14
Met Arg Phe Phe Ser Ala Leu Leu Ala Val Thr Ser Val Ala Ser Val
1 5 10 15
Phe Ala Arg Ala Leu Pro Pro Phe Ser Pro Ser Thr Gly Ala Asp Arg
20 25 30
Arg Gly Ala Phe Ser Val Lys Gln Val Arg Asn Leu Asn Phe Val Arg
35 40 45
Ser Gly Pro Val Gln Leu Ala Lys Ile Tyr His Lys Tyr His Ala Pro
50 55 60
Leu Pro Asp Asp Leu Lys Ala Ala Val Ser Arg Ile Arg Ser Asn Thr
65 70 75 80
Leu Lys Arg Ser Asn Gly Thr Val Glu Thr Asn Pro Glu Ala Asn Asp
85 90 95
Val Glu Tyr Leu Thr Pro Val Ser Ile Gly Thr Pro Glu Gln Val Leu
100 105 110
Asn Leu Asp Phe Asp Thr Gly Ser Ser Asp Leu Trp Val Phe Ser Ser
115 120 125
Gln Thr Lys Asn Ser Glu Val Asp Gly Gln Thr Val Tyr Asn Pro Thr
130 135 140
Lys Ser Ser Thr Ala Lys Glu Leu Lys Gly Tyr Thr Trp Gln Ile Ser
145 150 155 160
Tyr Gly Asp Gly Ser Tyr Ser Gly Gly Asn Val Tyr Thr Asp Thr Val
165 170 175
Thr Val Gly Gly Leu Ala Val Pro Ser Gln Ala Val Glu Val Ala Thr
180 185 190
Gln Val Ser Asp Glu Phe Thr Ser Asp Pro Asn Asn Asp Gly Leu Leu
195 200 205
Gly Leu Gly Phe Ser Ser Ile Asn Thr Val Glu Pro Lys Ser Gln Lys
210 215 220
Thr Phe Phe Asp Asn Ala Met Pro Asn Leu Asp Ser Pro Leu Phe Thr
225 230 235 240
Ala Asp Leu Lys Ala Ser Val Pro Gly His Phe Thr Phe Gly Tyr Ile
245 250 255
Asn Pro Asp Ala Tyr Ile Gly Asn Ile Thr Tyr Thr Pro Val Asp Ser
260 265 270
Ser Gly Gly Phe Trp Ala Trp Lys Ser Ser Gly Tyr Ala Val Gly Ser
275 280 285
Gly Ser Phe Lys Thr Thr Thr Phe Gln Gly Ile Ala Asp Thr Gly Thr
290 295 300
Ser Leu Leu Leu Leu Pro Ser Ser Val Val Ser Ala Tyr Tyr Arg Gln
305 310 315 320
Val Arg Asn Ala Thr Tyr Asp Ser Thr Gln Gly Gly Tyr Thr Leu Pro
325 330 335
Cys Ser Gly Pro Val Pro Asp Phe Ala Phe Gly Val Gly Asp Lys Asp
340 345 350
Thr Met Ile Thr Val Pro Gly Asp Tyr Ile Thr Tyr Ala Pro Thr Ser
355 360 365
Ser Ser Gly Glu Thr Cys Phe Gly Gly Ile Gln Pro Asp Thr Gly Ile
370 375 380
Gly Ile Ser Ile Tyr Gly Asp Val Ala Leu Lys Ala Ala Phe Val Val
385 390 395 400
Phe Asp Gly Gly Asn Met Gln Leu Gly Trp Ala Ser Lys Thr Leu Pro
405 410 415
Leu Ser Thr Gly Pro
420
<210> 15
<211> 439
<212> PRT
<213> 粗糙链孢霉
<400> 15
Met Val Ala Leu Thr Asn Leu Leu Leu Thr Thr Leu Leu Ala Ser Ala
1 5 10 15
Gly Leu Gly Ala Ala Leu Pro Pro Arg Ile Gly Ser Thr Val Ile Glu
20 25 30
Ala Arg Glu Pro Glu Leu Pro Val Ser Gly Arg Lys Ile Thr Leu Pro
35 40 45
Gln Gln Lys Asn Pro Arg Phe His Lys Phe Asn Gly Ala Leu Ser Val
50 55 60
Tyr Lys Thr Tyr Leu Lys Tyr Gly Ala Pro Val Pro Asp His Leu Val
65 70 75 80
Gln Ala Val Ala Asn His Leu Gly Ile Ser Val Glu Glu Val His Asn
85 90 95
Tyr Ala Asn Thr Thr Ala Asn Ala Arg Arg Asp Gln Gly Ser Ala Thr
100 105 110
Ala Ala Pro Ile Asp Gln Ser Asp Ser Ala Tyr Ile Thr Pro Val Ser
115 120 125
Ile Gly Thr Pro Ala Gln Thr Leu Asn Leu Asp Phe Asp Thr Gly Ser
130 135 140
Ser Asp Leu Trp Val Phe Ser Asn Ser Leu Pro Ser Ser Gln Arg Ala
145 150 155 160
Gly His Thr Ile Tyr Asn Pro Ser Lys Ser Ser Thr Ala Lys Arg Val
165 170 175
Asn Gly Ala Ser Trp Asp Ile Ser Tyr Gly Asp Gly Ser Ser Ser Lys
180 185 190
Gly Gln Val Tyr Leu Asp Lys Val Thr Ile Gly Gly Leu Val Val Ser
195 200 205
Asn Gln Ala Val Glu Thr Ala Gln Gln Val Ser Gln Ser Phe Thr Ala
210 215 220
Glu Thr Ser Ile Asp Gly Leu Val Gly Leu Ala Phe Gly Ser Leu Asn
225 230 235 240
Thr Val Arg Pro Arg Gln Gln Lys Thr Trp Phe Glu Asn Ala Ile Gly
245 250 255
Gln Leu Asp Gln Pro Leu Phe Ala Ala Asp Leu Lys Tyr Glu Ala Ser
260 265 270
Gly Thr Tyr Asp Phe Gly Phe Ile Asp Pro Ala Lys His Thr Gly Asp
275 280 285
Ile Thr Tyr Val Pro Val Asn Thr Asn Pro Gly Tyr Trp Thr Trp Thr
290 295 300
Ser Thr Gly Tyr Gln Val Gly Ser Ser Pro Phe Val Ser Gln Ser Ile
305 310 315 320
Thr Asn Ile Ala Asp Thr Gly Thr Thr Leu Met Tyr Val Pro Asp Ser
325 330 335
Ile Leu Arg Ala Tyr Tyr Gly Gln Ile Arg Gly Ala Thr Asn Ser Gln
340 345 350
Ser Tyr Gly Gly Tyr Val Phe Pro Cys Ser Thr Glu Ala Pro Asp Phe
355 360 365
Thr Phe Gly Val Thr Asp Glu Ala Thr Ile Thr Ile Pro Gly Arg Phe
370 375 380
Ile Asn Tyr Gly Pro Val Thr Asp Asp Gly Glu Thr Cys Phe Gly Gly
385 390 395 400
Leu Gln Thr Ser Ser Asp Val Gly Ile Asn Ile Phe Gly Asp Val Ala
405 410 415
Leu Lys Ala Ala Tyr Val Val Phe Lys Gly Gly Asp Ser Pro Ser Leu
420 425 430
Gly Trp Ala Ser Lys Gln Leu
435
<210> 16
<211> 430
<212> PRT
<213> 四孢链孢菌
<400> 16
Met Leu Leu Phe Pro Thr Ile Leu Thr Ala Ala Leu Ala Ala Thr Gly
1 5 10 15
Met Ala Ala Ala Ile Pro Ser Arg Asp Asp Thr Thr Ala Asn Lys Gly
20 25 30
Thr Ala Ser Leu Leu Gln Val Arg Asn Pro Ser Phe Glu Phe Arg His
35 40 45
Gly Pro Leu Ala Leu Ala Lys Ala Tyr Gln Lys Phe Gly Ala Pro Met
50 55 60
Pro Glu Asp Leu Arg Ala Ala Ile Ala Arg Phe Arg Gln Asn Gln Lys
65 70 75 80
Arg Thr Thr Gly Thr Ile Ala Thr Asp Pro Glu Lys His Asp Val Glu
85 90 95
Tyr Leu Thr Pro Ile Ser Val Gly Thr Pro Ser Gln Asp Leu Met Val
100 105 110
Asp Phe Asp Thr Gly Ser Ser Asp Leu Trp Val Phe Ser Thr Glu Met
115 120 125
Ser Thr Ser Asp Ile Lys Gly Gln Thr Val Tyr Asp Pro Asn Asn Ser
130 135 140
Ser Thr Ser Glu Lys Val Gln Gly Ser Thr Trp Lys Ile Thr Tyr Gly
145 150 155 160
Asp Gly Ser Ser Ser Ser Asp Asp Val Tyr Leu Asp Thr Val Thr Ile
165 170 175
Gly Asn Leu Thr Val Pro Asn Gln Ala Val Glu Ala Ala Lys Lys Val
180 185 190
Ser Ser Glu Phe Thr Asp Asp Ser His Asn Asp Gly Leu Leu Gly Leu
195 200 205
Gly Phe Ser Ala Ile Asn Ala Val Glu Pro Thr Pro Gln Asn Thr Phe
210 215 220
Phe Asp Asn Ile Lys Gly Ser Leu Asp Ala Pro Leu Phe Thr Val Asp
225 230 235 240
Leu Lys His Gly Thr Pro Gly Ser Phe Asn Phe Gly Tyr Ile Asp Pro
245 250 255
Thr Ala Tyr Ile Gly Asn Ile Ser Trp Thr Pro Val Asp Ser Ser Gln
260 265 270
Gly Tyr Trp Gly Phe Thr Ser Pro Gly Tyr Ala Val Gly Thr Gly Ala
275 280 285
Phe Arg Asn His Ser Met Ser Gly Ile Ala Asp Thr Gly Thr Thr Leu
290 295 300
Leu Leu Leu Pro Lys Ser Val Val Ser Ala Tyr Tyr Lys Glu Ile Gln
305 310 315 320
Gly Ala Gln Tyr Asp Ser Asp Gln Gly Gly Tyr Ile Phe Pro Cys Ser
325 330 335
Pro Thr Pro Pro Asp Phe Val Phe Gly Val Asn Asn Gly Ile Val Thr
340 345 350
Val Pro Gly Asp Met Val Ser Tyr Ala Pro Ala Asp Ser Ala Asn Gln
355 360 365
Asn Cys Phe Gly Gly Ile Gln Lys Asp Thr Gly Ile Gly Phe Ser Ile
370 375 380
Phe Gly Asp Val Ala Leu Lys Thr Ser Phe Val His Leu His Ser Ser
385 390 395 400
Ile Val Pro Gly His Tyr Ala Asp Cys Asp Met Arg Phe Asn Arg Met
405 410 415
Leu Arg Ser Tyr Leu Val Asp Phe Ser Ser Ser Gly Pro Leu
420 425 430
<210> 17
<211> 452
<212> PRT
<213> 里氏木霉
<400> 17
Met Glu Ala Ile Leu Gln Ala Gln Ala Lys Phe Arg Leu Asp Arg Gly
1 5 10 15
Leu Gln Lys Ile Thr Ala Val Arg Asn Lys Asn Tyr Lys Arg His Gly
20 25 30
Pro Lys Ser Tyr Val Tyr Leu Leu Asn Arg Phe Gly Phe Glu Pro Thr
35 40 45
Lys Pro Gly Pro Tyr Phe Gln Gln His Arg Ile His Gln Arg Gly Leu
50 55 60
Ala His Pro Asp Phe Lys Ala Ala Val Gly Gly Arg Val Thr Arg Gln
65 70 75 80
Lys Val Leu Ala Lys Lys Val Lys Glu Asp Gly Thr Val Asp Ala Gly
85 90 95
Gly Ser Lys Thr Gly Glu Val Asp Ala Glu Asp Gln Gln Asn Asp Ser
100 105 110
Glu Tyr Leu Cys Glu Val Thr Ile Gly Thr Pro Gly Gln Lys Leu Met
115 120 125
Leu Asp Phe Asp Thr Gly Ser Ser Asp Leu Trp Val Phe Ser Thr Glu
130 135 140
Leu Ser Lys His Leu Gln Glu Asn His Ala Ile Phe Asp Pro Lys Lys
145 150 155 160
Ser Ser Thr Phe Lys Pro Leu Lys Asp Gln Thr Trp Gln Ile Ser Tyr
165 170 175
Gly Asp Gly Ser Ser Ala Ser Gly Thr Cys Gly Ser Asp Thr Val Thr
180 185 190
Leu Gly Gly Leu Ser Ile Lys Asn Gln Thr Ile Glu Leu Ala Ser Lys
195 200 205
Leu Ala Pro Gln Phe Ala Gln Gly Thr Gly Asp Gly Leu Leu Gly Leu
210 215 220
Ala Trp Pro Gln Ile Asn Thr Val Gln Thr Asp Gly Arg Pro Thr Pro
225 230 235 240
Ala Asn Thr Pro Val Ala Asn Met Ile Gln Gln Asp Asp Ile Pro Ser
245 250 255
Asp Ala Gln Leu Phe Thr Ala Ala Phe Tyr Ser Glu Arg Asp Glu Asn
260 265 270
Ala Glu Ser Phe Tyr Thr Phe Gly Tyr Ile Asp Gln Asp Leu Val Ser
275 280 285
Ala Ser Gly Gln Glu Ile Ala Trp Thr Asp Val Asp Asn Ser Gln Gly
290 295 300
Phe Trp Met Phe Pro Ser Thr Lys Thr Thr Ile Asn Gly Lys Asp Ile
305 310 315 320
Ser Gln Glu Gly Asn Thr Ala Ile Ala Asp Thr Gly Thr Thr Leu Ala
325 330 335
Leu Val Ser Asp Glu Val Cys Glu Ala Leu Tyr Lys Ala Ile Pro Gly
340 345 350
Ala Lys Tyr Asp Asp Asn Gln Gln Gly Tyr Val Phe Pro Ile Asn Thr
355 360 365
Asp Ala Ser Ser Leu Pro Glu Leu Lys Val Ser Val Gly Asn Thr Gln
370 375 380
Phe Val Ile Gln Pro Glu Asp Leu Ala Phe Ala Pro Ala Asp Asp Ser
385 390 395 400
Asn Trp Tyr Gly Gly Val Gln Ser Arg Gly Ser Asn Pro Phe Asp Ile
405 410 415
Leu Gly Asp Val Phe Leu Lys Ser Val Tyr Ala Ile Phe Asp Gln Gly
420 425 430
Asn Gln Arg Phe Gly Ala Val Pro Lys Ile Gln Ala Lys Gln Asn Leu
435 440 445
Gln Pro Pro Gln
450
<210> 18
<211> 454
<212> PRT
<213> 深绿木霉
<400> 18
Met Glu Ala Ile Phe Asn Val Gln Ser Lys Phe Ala Leu Glu Arg Gly
1 5 10 15
Leu Ser Lys Ile Lys Ala Ile Arg Asn Glu Asn Tyr Lys Arg His Gly
20 25 30
Thr Lys Ser Tyr Val Tyr Leu Leu Asn Arg Phe Gly Phe Glu Pro Thr
35 40 45
Lys Pro Gly Pro Tyr Phe Gln Gly Ala Ser Phe His Gln Arg Gly Leu
50 55 60
Ala His Pro Asp Phe His Glu Ala Arg Gly Gly Arg Val His Lys Lys
65 70 75 80
Lys His Leu Lys Lys Lys Gln Thr Pro Glu Gly Ala Thr Asp Pro Asn
85 90 95
Gly Thr Glu Thr Gly Glu Val Gly Ala Glu Asp Gln Gln Asn Asp Ser
100 105 110
Glu Tyr Leu Cys Glu Val Thr Ile Gly Thr Pro Gly Gln Lys Leu Leu
115 120 125
Leu Asp Phe Asp Thr Gly Ser Ser Asp Leu Trp Val Phe Ser Thr Glu
130 135 140
Leu Ser Lys Ser Leu Gln Ser Asn His Thr Val Phe Asn Pro Lys Asn
145 150 155 160
Ser Ser Thr Phe Lys Ala Leu Thr Gly Gln Lys Trp Gln Ile Ser Tyr
165 170 175
Gly Asp Gly Ser Ser Ala Ser Gly Asp Cys Gly Ser Asp Asp Val Thr
180 185 190
Val Gly Gly Leu Thr Ile Lys Asn Gln Thr Val Glu Leu Ala Ser Thr
195 200 205
Leu Ala Gln Gln Phe Ala Gln Gly Thr Gly Asp Gly Leu Leu Gly Leu
210 215 220
Ala Trp Pro Ser Ile Asn Thr Val Thr Thr Asn Gly Lys Pro Thr Pro
225 230 235 240
Ala Asn Thr Pro Val Ala Asn Met Ile Thr Gln Glu Asp Val Pro Ser
245 250 255
Glu Ser Glu Leu Phe Thr Ala Ala Phe Tyr Ser Glu Arg Asp Ala Asn
260 265 270
Ala Gln Ser Phe Tyr Thr Phe Gly Tyr Ile Asp Gln Asp Leu Val Thr
275 280 285
Ala Ser Gly Gln Gln Ile Ala Trp Thr Asp Val Asp Asn Ser Gln Gly
290 295 300
Phe Trp Met Phe Pro Ser Thr Ser Ser Ser Val Asn Gly Lys Ala Ile
305 310 315 320
Ser Gln Ser Gly Asn Ser Ala Ile Ala Asp Thr Gly Thr Thr Leu Ala
325 330 335
Leu Val Ser Asp Asp Val Cys Asp Ala Leu Tyr Lys Ala Ile Pro Gly
340 345 350
Ala Lys Tyr Asp Asp Gln Gln Gln Gly Tyr Val Phe Pro Met Ser Thr
355 360 365
Asp Val Ser Ser Leu Pro Glu Phe Lys Val Ser Val Gly Asn Thr Gln
370 375 380
Phe Val Ile Gln Pro Glu Asp Leu Ala Phe Ala Pro Ala Asp Asn Asn
385 390 395 400
Asn Trp Tyr Gly Gly Val Gln Ser Arg Gly Asp Asn Pro Phe Asp Ile
405 410 415
Leu Gly Asp Val Phe Leu Lys Ser Val Tyr Ala Ile Phe Asp Gln Gly
420 425 430
Asn Gln Arg Phe Gly Ala Val Pro Lys Ile Gln Ala Thr Gln Asn Leu
435 440 445
Asn Pro Pro Ser Asn Gln
450
<210> 19
<211> 453
<212> PRT
<213> 绿木霉
<400> 19
Met Glu Ala Ile Phe Gln Ala Gln Ala Lys Phe Arg Leu Asp Arg Gly
1 5 10 15
Leu Thr Lys Val Lys Ala Ile Arg Asn Gln Asn Tyr Lys Arg His Gly
20 25 30
Thr Lys Ser Tyr Val Tyr Leu Leu Asn Arg Phe Gly Phe Glu Pro Thr
35 40 45
Lys Pro Gly Pro Tyr Tyr Gln Gln His Arg Ile Arg Gln Arg Gly Leu
50 55 60
Ala His Pro Asp Phe His Ala Pro Val Gly Gly Arg Val His His Thr
65 70 75 80
Lys His Leu His Lys Lys Val Asn Lys His Gly Ala Pro Asp Ala Thr
85 90 95
Gly Thr Glu Thr Gly Glu Val Gly Ala Glu Asp Gln Gln Asn Asp Ser
100 105 110
Glu Tyr Leu Cys Glu Val Thr Ile Gly Thr Pro Gly Gln Thr Leu Leu
115 120 125
Leu Asp Phe Asp Thr Gly Ser Ser Asp Leu Trp Val Phe Ser Thr Glu
130 135 140
Leu Ser Lys Thr Ile Gln Lys Gly His Ser Val Phe Gln Pro Thr Ser
145 150 155 160
Ser Ser Thr Phe Lys Lys Leu Pro Asn Gln Ala Trp Gln Ile Ser Tyr
165 170 175
Gly Asp Gly Ser Ser Ala Ser Gly Asp Cys Gly Ser Asp Asn Val Thr
180 185 190
Ile Gly Gly Leu Thr Ile Lys Asn Gln Thr Val Glu Leu Ala Ser Lys
195 200 205
Leu Ala Gln Gln Phe Ala Gln Gly Thr Gly Asp Gly Leu Leu Gly Leu
210 215 220
Ala Trp Pro Ala Ile Asn Thr Val Ala Thr Asn Gly Lys Ser Thr Pro
225 230 235 240
Ala Asn Thr Pro Val Ala Asn Met Ile Thr Gln Asp Asp Ile Pro Ser
245 250 255
Asp Ala Glu Leu Phe Thr Ala Ala Phe Tyr Ser Glu Arg Asp Ala Asn
260 265 270
Ala Gln Ser Phe Tyr Thr Phe Gly Tyr Ile Asp Thr Asp Leu Val Ser
275 280 285
Ser Ser Gly Gln Asp Ile Ala Trp Thr Asp Ile Asp Asn Ser Gln Gly
290 295 300
Phe Trp Met Phe Pro Ser Thr Ser Thr Ser Val Asn Gly Lys Ala Ile
305 310 315 320
Ser Gln Ser Gly Asn Ser Ala Ile Ala Asp Thr Gly Thr Thr Leu Ala
325 330 335
Leu Val Ser Asp Glu Val Cys Glu Ala Leu Tyr Asn Ala Ile Pro Gly
340 345 350
Ala Thr Tyr Asp Asp Gln Gln Gln Gly Tyr Val Phe Pro Gln Ser Thr
355 360 365
Asp Val Ser Ser Leu Pro Glu Phe Lys Val Ser Val Gly Asp Thr Gln
370 375 380
Phe Val Ile Gln Pro Glu Asp Leu Ala Phe Ala Pro Ala Asp Asp Asn
385 390 395 400
Asn Trp Tyr Gly Gly Ile Gln Ser Arg Gly Thr Asn Pro Phe Asp Ile
405 410 415
Leu Gly Asp Val Phe Leu Lys Ser Ile Tyr Ala Ile Phe Asp Gln Gly
420 425 430
Asn Gln Arg Phe Gly Ala Val Pro Lys Ile Gln Ala Ser Gln Asn Leu
435 440 445
Ser Ser Ser Ala Gln
450
<210> 20
<211> 454
<212> PRT
<213> 有性哈茨木霉
<400> 20
Met Glu Ala Ile Phe Gln Ala Gln Ala Lys Phe Arg Leu Asp Arg Gly
1 5 10 15
Leu His Lys Ile Thr Ala Ile Arg Asn Lys Asn Tyr Lys Arg His Gly
20 25 30
Thr Lys Ser Tyr Val Tyr Leu Leu Asn Arg Phe Gly Phe Glu Pro Thr
35 40 45
Lys Pro Gly Pro Tyr Phe Gln Glu His Arg Val His Gln Arg Gly Leu
50 55 60
Val His Pro Asp Tyr Asn Ala Pro Val Gly Gly Arg Val His Ile Thr
65 70 75 80
Lys Ala Leu His Lys Lys Lys Asn His His Gly Ala Leu Asp Ala Asn
85 90 95
Gly Ser Glu Lys Gly Glu Val Gly Ala Glu Asp Gln Gln Asn Asp Ser
100 105 110
Glu Tyr Leu Cys Glu Val Thr Ile Gly Thr Pro Gly Gln Lys Leu Leu
115 120 125
Leu Asp Phe Asp Thr Gly Ser Ser Asp Leu Trp Val Phe Ser Thr Glu
130 135 140
Leu Ser Lys Ser Leu Gln Lys Asn His Thr Ile Phe Asn Pro Ser Glu
145 150 155 160
Ser Ser Thr Phe Lys Lys Leu Ser Gly Gln Thr Trp Gln Ile Ser Tyr
165 170 175
Gly Asp Gly Ser Ser Ala Ser Gly Asp Cys Gly Ser Asp Asn Val Thr
180 185 190
Ile Gly Gly Leu Thr Ile Lys Asn Gln Thr Val Glu Leu Ala Ser Lys
195 200 205
Leu Ala Ala Gln Phe Ala Gln Gly Thr Gly Asp Gly Leu Leu Gly Leu
210 215 220
Ala Trp Pro Ser Ile Asn Thr Val Ala Thr Asn Gly Arg Ser Thr Pro
225 230 235 240
Ala Asn Thr Pro Val Ala Asn Met Ile Thr Gln Asp Asp Val Pro Ser
245 250 255
Asn Ala Glu Leu Phe Thr Ala Ala Phe Tyr Ser Glu Arg Asp Ala Asn
260 265 270
Ala Glu Ser Phe Tyr Thr Phe Gly Tyr Ile Asp Gln Asp Leu Val Thr
275 280 285
Ala Ser Gly Gln Asp Ile Ala Trp Thr Asp Val Asp Asn Ser Gln Gly
290 295 300
Phe Trp Met Phe Pro Ser Thr Ser Ser Ser Val Asn Gly Gln Ala Ile
305 310 315 320
Ser Gln Ser Gly Asn Ser Ala Ile Ala Asp Thr Gly Thr Thr Leu Ala
325 330 335
Leu Val Ser Asp Glu Val Cys Asp Ala Leu Tyr Lys Ala Ile Pro Gly
340 345 350
Ala Thr Tyr Asp Asp Gln Gln Gln Gly Tyr Val Phe Pro Met Ser Thr
355 360 365
Asp Val Ser Ser Leu Pro Glu Phe Lys Val Ser Val Gly Asp Thr Gln
370 375 380
Phe Val Ile Gln Pro Glu Asp Leu Ala Phe Ala Pro Ala Asp Asp Asn
385 390 395 400
Asn Trp Tyr Gly Gly Val Gln Ser Arg Gly Ser Asn Pro Phe Asp Ile
405 410 415
Leu Gly Asp Val Phe Leu Lys Ser Val Tyr Ala Ile Phe Asp Gln Gly
420 425 430
Asn Gln Arg Phe Gly Ala Val Pro Lys Ile Gln Ala Thr Gln Asn Leu
435 440 445
Asp Ser Ser Ser Ala Gln
450
<210> 21
<211> 454
<212> PRT
<213> 棘孢木霉
<400> 21
Met Glu Ala Ile Phe Asn Val Gln Thr Lys Phe Arg Leu Glu Arg Gly
1 5 10 15
Leu Ser Lys Ile Arg Ala Ile Arg Asn Glu Asn Tyr Lys Arg His Gly
20 25 30
Thr Lys Ser Tyr Val Tyr Leu Leu Asn Arg Phe Gly Phe Glu Pro Thr
35 40 45
Lys Pro Gly Pro Tyr Phe Gln Gly Val Gln Phe His Gln Arg Gly Leu
50 55 60
Ala His Pro Asp Phe His Glu Ala Arg Gly Gly Arg Val His Lys Lys
65 70 75 80
Lys His Leu Arg Lys Lys Val Thr Pro Glu Gly Ser Thr Asp Pro Asn
85 90 95
Gly Thr Glu Thr Gly Glu Val Gly Ala Glu Asp Gln Gln Asn Asp Ser
100 105 110
Glu Tyr Leu Cys Glu Val Thr Ile Gly Thr Pro Gly Gln Thr Leu Leu
115 120 125
Leu Asp Phe Asp Thr Gly Ser Ser Asp Leu Trp Val Phe Ser Thr Glu
130 135 140
Leu Ser Ser Thr Ile Gln Lys Gly His Thr Val Phe Asn Pro Lys Asn
145 150 155 160
Ser Ser Thr Phe Lys Ala Leu Thr Gly Gln Lys Trp Gln Ile Ser Tyr
165 170 175
Gly Asp Gly Ser Ser Ala Ser Gly Asp Cys Gly Ser Asp Asn Val Thr
180 185 190
Val Gly Gly Leu Thr Ile Lys Asn Gln Thr Val Glu Leu Ala Ser Thr
195 200 205
Leu Ala Gln Gln Phe Ser Gln Gly Thr Gly Asp Gly Leu Leu Gly Leu
210 215 220
Ala Trp Pro Ser Ile Asn Thr Val Thr Thr Asp Gly Lys Pro Thr Pro
225 230 235 240
Ala Asn Thr Ser Arg Cys Gln His Ile Thr Gln Asp Asp Val Pro Ser
245 250 255
Glu Ala Gln Leu Phe Thr Ala Ala Phe Tyr Ser Glu Arg Asp Ala Asn
260 265 270
Ala Gln Ser Phe Tyr Thr Phe Gly Tyr Ile Asp Asn Asp Leu Val Thr
275 280 285
Ala Ser Gly Gln Asp Ile Ala Trp Thr Asp Val Asp Asn Ser Gln Gly
290 295 300
Phe Trp Met Phe Pro Ser Thr Ser Ser Ser Ile Asn Gly Lys Ala Ile
305 310 315 320
Ser Gln Ser Gly Asn Ser Ala Ile Ala Asp Thr Gly Thr Thr Leu Ala
325 330 335
Leu Val Ser Asp Glu Val Cys Asp Ala Leu Tyr Lys Ala Ile Pro Gly
340 345 350
Ala Lys Tyr Asp Asp Gln Gln Gln Gly Tyr Val Phe Pro Met Ser Thr
355 360 365
Asp Val Ser Ser Leu Pro Glu Phe Lys Val Ser Val Gly Asp Thr Gln
370 375 380
Phe Val Ile Gln Pro Glu Asp Leu Ala Phe Ala Pro Ala Asp Asn Ser
385 390 395 400
Asn Trp Tyr Gly Gly Val Gln Ser Arg Gly Asn Asn Pro Phe Asp Ile
405 410 415
Leu Gly Asp Val Phe Leu Lys Ser Val Tyr Ala Ile Phe Asp Gln Gly
420 425 430
Asn Gln Arg Phe Gly Ala Val Pro Lys Ile Gln Ala Thr Gln Asn Leu
435 440 445
Asn Ser Ser Ser Asn Gln
450
<210> 22
<211> 426
<212> PRT
<213> 黑曲霉
<400> 22
Met Ala Thr Lys Ile Lys Leu Ile Pro Asn Leu Asn Tyr Lys Arg Ser
1 5 10 15
Gly Thr Lys Ser Tyr Val His Leu Met Arg Lys Tyr Arg Phe His Pro
20 25 30
Thr Lys Pro Gly Pro Tyr Thr Leu Ser Ser Ser Ile Gln Gln Thr Gly
35 40 45
Arg Pro Tyr Thr Glu Lys Pro Ile Gly Gly Arg Ala His Ile Arg Gln
50 55 60
Leu Val Arg Lys Lys Ser Thr Thr Ser Asp Glu Val Gly Glu Val Pro
65 70 75 80
Ala Glu Asp Val Gln Asn Asp Ser Met Tyr Leu Ala Thr Val Gly Ile
85 90 95
Gly Thr Pro Ala Gln Asn Leu Lys Leu Asp Phe Asp Thr Gly Ser Ala
100 105 110
Asp Leu Trp Val Trp Ser Asn Lys Leu Pro Ser Thr Leu Leu Ser Glu
115 120 125
Asn Lys Thr His Ala Ile Phe Asp Ser Ser Lys Ser Ser Thr Phe Lys
130 135 140
Thr Leu Glu Gly Glu Ser Trp Gln Ile Ser Tyr Gly Asp Gly Ser Ser
145 150 155 160
Ala Ser Gly Ser Val Gly Thr Asp Asp Val Asn Ile Gly Gly Val Val
165 170 175
Val Lys Asn Gln Ala Val Glu Leu Ala Glu Lys Met Ser Ser Thr Phe
180 185 190
Ala Gln Gly Glu Gly Asp Gly Leu Leu Gly Leu Ala Phe Ser Asn Ile
195 200 205
Asn Thr Val Gln Pro Lys Ser Val Lys Thr Pro Val Glu Asn Met Ile
210 215 220
Leu Gln Asp Asp Ile Pro Lys Ser Ala Glu Leu Phe Thr Ala Lys Leu
225 230 235 240
Asp Thr Trp Arg Asp Thr Asp Asp Glu Ser Phe Tyr Thr Phe Gly Phe
245 250 255
Ile Asp Gln Asp Leu Val Lys Thr Ala Gly Glu Glu Val Tyr Tyr Thr
260 265 270
Pro Val Asp Asn Ser Gln Gly Phe Trp Leu Phe Asn Ser Thr Ser Ala
275 280 285
Thr Val Asn Gly Lys Thr Ile Asn Arg Ser Gly Asn Thr Ala Ile Ala
290 295 300
Asp Thr Gly Thr Thr Leu Ala Leu Val Asp Asp Asp Thr Cys Glu Ala
305 310 315 320
Ile Tyr Ser Ala Ile Asp Gly Ala Tyr Tyr Asp Gln Glu Val Gln Gly
325 330 335
Trp Ile Tyr Pro Thr Asp Thr Ala Gln Asp Lys Leu Pro Thr Val Ser
340 345 350
Phe Ala Val Gly Glu Lys Gln Phe Val Val Gln Lys Glu Asp Leu Ala
355 360 365
Phe Ser Glu Ala Lys Thr Gly Tyr Val Tyr Gly Gly Ile Gln Ser Arg
370 375 380
Gly Asp Met Thr Met Asp Ile Leu Gly Asp Thr Phe Leu Lys Ser Ile
385 390 395 400
Tyr Ala Ile Phe Asp Val Gly Asn Leu Arg Phe Gly Ala Val Gln Arg
405 410 415
Glu Glu Leu Arg Gln Ser Leu Lys Ser Glu
420 425
<210> 23
<211> 459
<212> PRT
<213> 烟曲霉
<400> 23
Met Ser Ser Cys Arg Phe Ser Asn Thr Leu Lys Thr Ala Thr Ala Ser
1 5 10 15
Pro Cys Lys Tyr Leu His Thr Thr Thr Thr Ile Thr Met Ala Thr Arg
20 25 30
Ile Lys Leu Leu Pro Asn Pro His Tyr Arg Lys Ser Gly Thr Lys Ser
35 40 45
Tyr Val His Leu Met Arg Lys Tyr Arg Phe Thr Pro Thr Lys Gly Gly
50 55 60
Arg Tyr Phe Leu Ser Ser Ser Leu His Gln Thr Gly Arg Gln Tyr Thr
65 70 75 80
His Leu Pro Val Gly Gly Arg Ala Arg Ile Gln His Val Leu Arg Lys
85 90 95
Arg Ile Ala Asp Thr Asp Glu Thr Ser Asp Val Gly Ala Asp Asp Val
100 105 110
Gln Asn Asp Thr Met Tyr Leu Ala Pro Val Ser Ile Gly Thr Pro Ala
115 120 125
Gln Thr Ile His Leu Glu Val Asp Thr Gly Ser Ala Asp Leu Trp Val
130 135 140
Trp Ser Thr His Leu Ala Pro Ala Thr Ile Ala Gln His Lys Thr Gly
145 150 155 160
Thr Val Phe Asp Pro Ser Lys Ser Ser Thr Phe Glu Glu Gln Ser Gly
165 170 175
Ser Thr Trp Lys Ile Ser Arg Asp Asp Gly Ser Phe Ala Phe Gly Thr
180 185 190
Val Gly Thr Asp Thr Ile Ser Leu Gly Gly Leu Arg Val Glu His Gln
195 200 205
Ala Ile Glu Leu Ala Asp Ser Leu Ser Ala Gln Tyr Glu Gln Gly Thr
210 215 220
Gly Asp Gly Leu Leu Gly Leu Ala Phe Ser His Ile Asn Thr Val Arg
225 230 235 240
Pro Gln Pro Val Arg Thr Leu Val Glu Asn Met Val Ala Gln Glu Asp
245 250 255
Ile Pro Glu Ser Ala Lys Leu Phe Thr Ala Lys Leu Gly Cys Trp Gln
260 265 270
Gly Ala Asn Glu Pro Asp Glu Gly Glu Pro Phe Phe Thr Phe Gly Phe
275 280 285
Ile Asp Gln Asp Ile Val Thr Ala Ser Gly Glu Glu Val Tyr Tyr Thr
290 295 300
Pro Ile Asp Asn Ser Arg Gly Phe Trp Leu Phe Asp Ser Ala Ser Ala
305 310 315 320
Thr Val Asn Gly Lys Thr Ile Ala Arg Ser Gly Asn Lys Ala Ile Ala
325 330 335
Asp Thr Gly Thr Pro Leu Ala Leu Val Asp Asp Glu Thr Cys Gln Ala
340 345 350
Ile Tyr Asp Ala Ile Pro Gly Ala His Tyr Asp Asp Glu Ser Gln Gly
355 360 365
Tyr Ile Phe Pro Ser Thr Thr Thr Ala Asp Lys Leu Pro Val Val Ser
370 375 380
Leu Ala Val Gly Asp Lys Gln Phe Val Val Gln Arg Glu Asp Leu Gly
385 390 395 400
Phe Ala Glu Ala Lys Ser Gly Tyr Val Tyr Gly Gly Ile Gln Ser Arg
405 410 415
Gly Ser Met Ala Met Asp Ile Leu Gly Ser Thr Phe Leu Lys Gly Ile
420 425 430
Tyr Pro Ile Phe Asp Val Gly Asn Leu Arg Phe Gly Ala Val Gln Arg
435 440 445
Lys Glu Leu His Gln Asn Leu Ser Ala Pro Gln
450 455
<210> 24
<211> 393
<212> PRT
<213> 黑曲霉
<400> 24
Met Arg Lys Tyr Arg Phe His Pro Thr Lys Pro Gly Pro Tyr Thr Leu
1 5 10 15
Ser Ser Ser Ile Gln Gln Thr Gly Arg Pro Tyr Thr Glu Lys Pro Ile
20 25 30
Gly Gly Arg Ala His Ile Arg Gln Leu Val Arg Lys Lys Ser Thr Thr
35 40 45
Ser Asp Glu Val Gly Glu Val Pro Ala Glu Asp Val Gln Asn Asp Ser
50 55 60
Met Tyr Leu Ala Thr Val Gly Ile Gly Thr Pro Ala Gln Asn Leu Lys
65 70 75 80
Leu Asp Phe Asp Thr Gly Ser Ala Asp Leu Trp Val Trp Ser Asn Lys
85 90 95
Leu Pro Ser Thr Leu Leu Ser Glu Asn Lys Thr His Ala Ile Phe Asp
100 105 110
Ser Ser Lys Ser Ser Thr Phe Lys Thr Leu Glu Gly Glu Ser Trp Gln
115 120 125
Ile Ser Tyr Gly Asp Gly Ser Ser Ala Ser Gly Ser Val Gly Thr Asp
130 135 140
Asp Val Asn Ile Gly Gly Val Val Val Lys Asn Gln Ala Val Glu Leu
145 150 155 160
Ala Glu Lys Met Ser Ser Thr Phe Ala Gln Gly Glu Gly Asp Gly Leu
165 170 175
Leu Gly Leu Ala Phe Ser Asn Ile Asn Thr Val Gln Pro Lys Ser Val
180 185 190
Lys Thr Pro Val Glu Asn Met Ile Leu Gln Asp Asp Ile Pro Lys Ser
195 200 205
Ala Glu Leu Phe Thr Ala Lys Leu Asp Thr Trp Arg Asp Thr Asp Asp
210 215 220
Glu Ser Phe Tyr Thr Phe Gly Phe Ile Asp Gln Asp Leu Val Lys Thr
225 230 235 240
Ala Gly Glu Glu Val Tyr Tyr Thr Pro Val Asp Asn Ser Gln Gly Phe
245 250 255
Trp Leu Phe Asn Ser Thr Ser Ala Thr Val Asn Gly Lys Thr Ile Asn
260 265 270
Arg Ser Gly Asn Thr Ala Ile Ala Asp Thr Gly Thr Thr Leu Ala Leu
275 280 285
Val Asp Asp Asp Thr Cys Glu Ala Ile Tyr Ser Ala Ile Asp Gly Ala
290 295 300
Tyr Tyr Asp Gln Glu Val Gln Gly Trp Ile Tyr Pro Thr Asp Thr Ala
305 310 315 320
Gln Asp Lys Leu Pro Thr Val Ser Phe Ala Val Gly Glu Lys Gln Phe
325 330 335
Val Val Gln Lys Glu Asp Leu Ala Phe Ser Glu Ala Lys Thr Gly Tyr
340 345 350
Val Tyr Gly Gly Ile Gln Ser Arg Gly Asp Met Thr Met Asp Ile Leu
355 360 365
Gly Asp Thr Phe Leu Lys Ser Ile Tyr Ala Val Ser Ala Leu Leu Leu
370 375 380
Ala Leu Arg Gly Asp Ile Glu Ala His
385 390
<210> 25
<211> 439
<212> PRT
<213> 蛹虫草
<400> 25
Met Glu Ala Phe Phe Gln Ala Gln Ala Lys Phe Gln Ala Ser Pro Gly
1 5 10 15
Leu Thr Lys Val Arg Ala Lys Pro Asn Gln Asn Tyr Lys Arg His Gly
20 25 30
Thr Lys Ser Tyr Val Tyr Leu Leu Asn Arg Phe Gly Phe Glu Pro Thr
35 40 45
Lys Pro Gly Pro Tyr Arg Arg Val Ser His Leu Gln Gln Arg Gly Leu
50 55 60
Ala Gln Ser His Val Ala Val Gly Gly Arg Ala Ala Val Thr Ser Arg
65 70 75 80
Leu Ala Lys Thr Leu Asp Ala Ala Gly Thr Gln Thr Gly Glu Val Thr
85 90 95
Ala Glu Asp Gln Gln Asn Asp Ser Glu Tyr Leu Cys Glu Val Ser Ile
100 105 110
Gly Thr Pro Pro Gln Lys Val Leu Leu Asp Phe Asp Thr Gly Ser Ser
115 120 125
Asp Leu Trp Ile Phe Ser Thr Glu Leu Ser Lys Ala Thr Gln Lys Gly
130 135 140
His Thr Ile Phe Asn Pro Ser Lys Ser Ser Thr Phe Lys Lys Leu Pro
145 150 155 160
Gly Gln Thr Trp Gln Ile Ser Tyr Gly Asp Gly Ser Ser Ala Ser Gly
165 170 175
Asp Cys Gly Ser Asp Asn Val Thr Ile Gly Gly Leu Thr Ile Glu Gly
180 185 190
Gln Thr Val Glu Leu Ala Ser Lys Leu Ala Ala Gln Phe Ala Gln Gly
195 200 205
Thr Gly Asp Gly Leu Leu Gly Leu Ala Phe Pro Ala Ile Asn Thr Val
210 215 220
Lys Lys Asn Gly Gln Pro Ala Pro Ala Gln Thr Pro Val Ala Asn Met
225 230 235 240
Met Thr Gln Gly Asp Ile Pro Lys Gln Ser Gln Leu Phe Thr Ser Ala
245 250 255
Phe Tyr Ser Glu Arg Asp Ala Asp Gly Pro Gln Ser Phe Tyr Thr Phe
260 265 270
Gly Tyr Val Asp Glu Ala Leu Val Lys Ala Ser Gly Lys Asp Ile Ala
275 280 285
Trp Thr Lys Ile Asp Ser Ser Glu Gly Phe Trp Met Phe Pro Ser Ala
290 295 300
Ser Val Ser Ile Ala Gly Lys Lys Leu Ala Leu Asp Gly Asn Thr Ala
305 310 315 320
Ile Ala Asp Thr Gly Thr Thr Leu Ala Leu Val Ser Asp Glu Val Cys
325 330 335
Asp Ala Leu Tyr Lys Ala Ile Pro Gly Ala Lys Tyr Asp Ala Gln Gln
340 345 350
Gln Gly Tyr Val Phe Pro Leu Thr Thr Lys Pro Glu Asp Leu Pro Ala
355 360 365
Phe Glu Val Ala Ala Glu Asp Leu Ala Phe Ala Gln Ala Asp Asp Lys
370 375 380
Asn Trp Tyr Gly Gly Val Gln Ser Arg Gly Ser Asn Pro Phe Asp Ile
385 390 395 400
Leu Gly Asp Ala Phe Leu Lys Ser Val Tyr Ala Ile Trp Asp Gln Gly
405 410 415
Asn Gln Arg Phe Gly Ala Val Pro Lys Ile Glu Lys Thr Gln Asn Leu
420 425 430
Thr Ala Lys Ala Gln Ser Gln
435
<210> 26
<211> 447
<212> PRT
<213> Glomerella graminicola
<400> 26
Met Gln Ala Phe Phe Gln Ala Gln Ala Lys Phe Lys Ala Asp Asn Gly
1 5 10 15
Leu Ala Lys Ile Pro Val Val Pro Asn Lys Asn Tyr Asn Arg His Gly
20 25 30
Arg Lys Ser Tyr Val His Leu Leu Asn Lys Phe Lys Phe Glu Pro Thr
35 40 45
Lys Pro Gly Pro Tyr Val Gln Thr Arg Arg Met Ala Gln Arg Gly Leu
50 55 60
Ala Ala Pro Gly Phe Asn Ala Pro Val Gly Gly Arg Val Ser Met Ser
65 70 75 80
Lys Val Leu Val Lys Lys Thr Glu Gly Asp Gln His Gly Glu Val Thr
85 90 95
Ala Glu Asp Gln Gln Tyr Asp Ser Met Tyr Leu Cys Glu Val Ser Ile
100 105 110
Gly Thr Pro Pro Gln Lys Phe Lys Leu Asp Phe Asp Thr Gly Ser Ser
115 120 125
Asp Leu Trp Val Phe Ser Lys Glu Leu Ser Ser Ser Leu Gln Arg Asn
130 135 140
His Asn Val Phe Asp Pro Ser Lys Ser Ser Thr Phe Lys Lys Leu Glu
145 150 155 160
Gly Lys Thr Trp Lys Ile Ser Tyr Gly Asp Gly Ser Ser Ala Ser Gly
165 170 175
Asp Cys Gly Thr Asp Asn Leu Val Leu Gly Gly Leu Thr Val Lys Asn
180 185 190
Gln Val Thr Glu Leu Ala Ala His Leu Ala Pro Gln Phe Ser Gln Gly
195 200 205
Thr Gly Asp Gly Leu Leu Gly Leu Ala Phe Ser Ser Ile Asn Thr Val
210 215 220
Gln Asn Lys Gly Gly Ala Arg Asp Pro Gln Ala Thr Ala Val Asp Asn
225 230 235 240
Met Ile Ala Glu Ser Asp Ile Pro Lys Asp Ala Glu Leu Phe Thr Ser
245 250 255
Ala Phe Tyr Ser Glu Arg Asp Glu Ser Lys Pro Arg Ser Phe Tyr Thr
260 265 270
Phe Gly Trp Ile Asp Thr Glu Leu Val Glu Ala Ser Gly Glu Asp Ile
275 280 285
His Trp Val Asp Ile Asp Asn Thr Gln Gly Phe Trp Met Phe Ser Ser
290 295 300
Glu Ser Ala Ser Val Asn Gly Lys Ala Val Ala Gln Ser Gly Asn Lys
305 310 315 320
Ala Ile Ala Asp Thr Gly Thr Thr Leu Ala Leu Val Ser Asp Glu Val
325 330 335
Cys Gln Ala Leu Tyr Asp Ala Ile Pro Gly Ser Lys Tyr Asp Glu Gln
340 345 350
Gln Gln Gly Tyr Val Phe Pro Thr Ser Ile Lys Ala Glu Asp Met Pro
355 360 365
Glu Phe Lys Val Ala Val Gly Asp Lys Glu Phe Val Ile Gln Lys Glu
370 375 380
Asp Leu Ala Phe Ala Pro Ala Ser Asp Gly Phe Trp Tyr Gly Gly Val
385 390 395 400
Gln Ser Arg Gly Ser Asn Lys Phe Asp Ile Leu Gly Asp Ala Phe Leu
405 410 415
Lys Ser Ile Tyr Ala Ile Trp Asp Gln Gly Asn Thr Arg Phe Gly Cys
420 425 430
Val Pro Lys Ile Glu Lys Thr Gln Asn Leu Gln Pro Ser Ser Ser
435 440 445
<210> 27
<211> 461
<212> PRT
<213> 尖孢镰刀菌
<400> 27
Met Glu Ala Ile Tyr Gln Val Gln His Gln Phe Arg Leu Asp Arg Gly
1 5 10 15
Leu Thr Arg Ile Ser Val Lys Arg Asn Pro His Tyr Gln Pro His Gly
20 25 30
Thr Lys Ser Tyr Val His Leu Leu Asn Arg Phe Gly Phe Lys Pro Thr
35 40 45
Lys Pro Gly Pro Tyr Phe Gln Asp Arg Gln Ile Gln Gln Arg Gly Leu
50 55 60
Ala His Pro Arg Phe His Ala Ala Val Gly Gly Arg Val Tyr Gln Thr
65 70 75 80
Lys Ile Leu Arg Lys Lys Leu Gly Thr Asp Gly Thr Leu Asp Ala Asp
85 90 95
Gly Thr Gln Ile Gly Glu Val Thr Ala Glu Asp Leu Pro Phe Asp Ser
100 105 110
Met Tyr Leu Cys Glu Val Ser Ile Gly Thr Pro Pro Gln Lys Phe Asn
115 120 125
Leu Asp Phe Asp Thr Gly Ser Ala Asp Leu Trp Val Asn Ile Pro Asn
130 135 140
Glu Ala Leu Lys Gln Arg Val Lys Asn Thr Asp Gln Lys Gly His Asn
145 150 155 160
Val Phe Asn Pro Leu Ser Ser Ser Ser Phe Asn Glu Leu Thr Asp Lys
165 170 175
Thr Trp Lys Ile Ser Tyr Gly Asp Gly Ser Ser Ala Ser Gly Asp Cys
180 185 190
Gly Ser Asp Asp Val Thr Ile Gly Gly Leu Thr Ile Lys Asn Gln Thr
195 200 205
Val Glu Leu Ala Ser Gln Leu Asp Gln Gln Phe Ala Arg Gly Lys Gly
210 215 220
Asp Gly Leu Leu Gly Leu Ala Phe Pro Gln Ile Asn Thr Val Lys Thr
225 230 235 240
Asn Trp Asp Ser Asp Pro Ala Asp Thr Pro Val Val Asn Met Ile Asn
245 250 255
Gln His Asp Ile Pro Lys Glu Ala Glu Leu Phe Thr Ser Ala Phe Tyr
260 265 270
Ser Gln Arg Asp Ala Asn Ser Pro Glu Ser Phe Tyr Thr Phe Gly Phe
275 280 285
Ile Asp Thr Asp Leu Val Ala Arg Ser Gly Glu Glu Ile Ser Trp Thr
290 295 300
Arg Ile Asp Ser Ser Asp Gly Phe Trp Lys Phe Pro Ser Ala Arg Phe
305 310 315 320
Ser Val Lys Gly Lys Ile Ile Asp Leu Ser Gly Asn Lys Ala Ile Ala
325 330 335
Asp Thr Gly Thr Thr Leu Val Leu Val Ser Asp Glu Val Cys Glu Ala
340 345 350
Leu Tyr Asp Ala Ile Pro Gly Ala Arg Tyr Ser Pro Thr Gln Gln Gly
355 360 365
Tyr Val Phe Pro Arg Ser Thr Asn Val Glu Asp Leu Pro Glu Phe Lys
370 375 380
Val Ala Ile Gly Asp Lys Gln Phe Asp Ile Gln Pro Gln Asp Leu Val
385 390 395 400
Phe Ala Pro Ala Asp Lys Asp Asn Trp Tyr Gly Gly Ile Gln Ser Arg
405 410 415
Gly Asn Leu Pro Phe Asp Ile Phe Gly Asp Ala Phe Leu Lys Ser Val
420 425 430
Tyr Ala Ile Trp Asp Gln Gly Asn Lys Arg Phe Gly Val Val Pro Lys
435 440 445
Ile Glu Glu Thr Gln Asn Leu Asp Pro Thr Pro Ile Ala
450 455 460
<210> 28
<211> 452
<212> PRT
<213> Grosmannia clavigera
<400> 28
Met Gln Ser Ile Phe Gln Val Gln Arg Gln Val Arg Ala Asp Leu Gly
1 5 10 15
Leu Glu Lys Val Arg Val Ile Leu Asn Lys Asn Tyr Lys Ala Asn Gly
20 25 30
Thr Lys Ser Tyr Val His Leu Leu Asn Arg Tyr Gly Phe Gln Pro Thr
35 40 45
Lys Pro Gly Pro Tyr Arg His Val Asn Arg Val His Gln Arg Gly Leu
50 55 60
Arg Val Thr Asp Ile Thr Lys Val His Lys Pro Val Gly Gly Arg Val
65 70 75 80
His Leu Glu Lys Val Leu Ala Lys His Ser Ala Thr Gly Asp Gly Asn
85 90 95
Thr Gln Ala Gly Glu Val Thr Ala Glu Asp Gln Gln Asn Asp Ser Met
100 105 110
Tyr Leu Cys Glu Val Glu Ile Gly Thr Pro Ser Gln Lys Leu Met Leu
115 120 125
Asp Phe Asp Thr Gly Ser Ser Asp Leu Trp Val Phe Ser Thr Glu Leu
130 135 140
Ser Ala Asp Val Gln Lys Gly His Ala Val Phe Asp Ser Ser Lys Ser
145 150 155 160
Thr Thr Phe Lys Thr Ser Ser Gly Leu Thr Trp Gln Ile Glu Tyr Gly
165 170 175
Asp Gly Ser Ser Ala Ser Gly Asp Val Gly Thr Asp Val Val Thr Val
180 185 190
Gly Gly Leu Val Ile Lys Asn Gln Ser Val Glu Leu Ala Lys Thr Leu
195 200 205
Ala Ala Gln Phe Ser Gln Gly Thr Gly Asp Gly Leu Leu Gly Leu Ala
210 215 220
Trp Pro Ser Ile Asn Thr Ile Lys Ser Gly Tyr Ser Ser Asp Pro Gln
225 230 235 240
Pro Thr Pro Val Ala Asn Met Ile Ser Glu Gly Val Val Pro Lys Glu
245 250 255
Ala Gln Leu Phe Thr Ser Cys Phe Tyr Gly Glu Arg Asp Asn Gln Glu
260 265 270
Ser Phe Tyr Thr Phe Gly Tyr Ile Asp Glu Asp Leu Val Lys Ala Ser
275 280 285
Gly Ala Glu Ile Ala Trp Thr Ser Ile Asp Asn Ser Gln Gly Phe Trp
290 295 300
Met Phe Pro Ser Thr Ser Ala Ser Ile Asn Gly Gln Ser Ile Thr Leu
305 310 315 320
Ser Asn Asn Thr Ala Ile Ala Asp Thr Gly Thr Thr Leu Ala Leu Val
325 330 335
Ser Asp Glu Val Cys Glu Ala Leu Tyr Lys Ala Ile Pro Asn Ser Lys
340 345 350
Tyr Asp Glu Lys Gln Gln Gly Tyr Leu Ile Pro Lys Thr Thr Thr Ile
355 360 365
Asp Gln Leu Pro Thr Val Ser Ile Thr Val Gly Asp Asn Lys Phe Val
370 375 380
Val Gln Lys Glu Asp Leu Ile Phe Thr Gln Ala Asp Asp Thr His Trp
385 390 395 400
Tyr Gly Gly Ile Gln Ser Arg Gly Ser Asn Thr Phe Asp Ile Leu Gly
405 410 415
Asp Val Phe Leu Lys Ser Ile Tyr Ala Ile Trp Asp Gln Gly Asn Val
420 425 430
Arg Phe Gly Ala Val Pro Lys Ile Gln Lys Thr Gln Thr Leu Asp Pro
435 440 445
Gly Ser Ser Thr
450
<210> 29
<211> 432
<212> PRT
<213> 灰葡萄孢霉
<400> 29
Met Ala Ser Leu Gln Gly Leu Ser Lys Ile Arg Leu Ile Pro Asn Lys
1 5 10 15
Asn Tyr Lys Arg Ser Gly Thr Lys Ser Tyr Val Tyr Leu Leu Asn Lys
20 25 30
Trp Gly Phe Glu Pro Thr Lys Pro Gly Pro Tyr Phe Gln Met Asn Lys
35 40 45
Ala Thr Ala Thr Ser Ala Ser Phe His Lys Phe Gly His Lys Ser Gln
50 55 60
Thr Gln Arg Val Leu Ala Lys Lys Thr Ala Thr Gly Glu Asn Gly Glu
65 70 75 80
Val Pro Ala Glu Asp Gln Gln Asn Asp Ser Glu Tyr Leu Cys Pro Val
85 90 95
Gln Ile Gly Thr Pro Ala Gln Thr Leu Met Leu Asp Phe Asp Thr Gly
100 105 110
Ser Ser Asp Leu Trp Val Trp Ser Thr Glu Leu Pro Lys Ala Thr Thr
115 120 125
Ser Asn Ala Thr Gly His Thr Ile Phe Asp Pro Lys Lys Ser Ser Thr
130 135 140
Phe Lys Ala Ala Lys Ser Ser Lys Trp Gln Ile Ser Tyr Gly Asp Ser
145 150 155 160
Ser Ser Ala Ser Gly Thr Val Gly Thr Asp Thr Val Ser Leu Gly Gly
165 170 175
Leu Ala Ile Lys Asn Gln Ala Val Glu Leu Ala Thr Lys Leu Ser Ala
180 185 190
Gln Phe Glu Gln Gly Ala Gly Asp Gly Leu Leu Gly Leu Ala Trp Gly
195 200 205
Ser Ile Asn Thr Val Thr Pro Thr Pro Val Ala Thr Pro Val Glu Asn
210 215 220
Met Ile Ser Gln Glu Asp Ile Pro Ser Asp Ala Glu Leu Phe Thr Val
225 230 235 240
Asn Leu Gly Ser Trp Arg Asp Ala Asp Glu Ala Asp Lys Gly Ala Ser
245 250 255
Phe Tyr Thr Phe Gly Tyr Ile Asp Gln Asp Val Val Gly Ser Gln Glu
260 265 270
Ile Tyr Tyr Thr Pro Val Asp Asn Ser Gln Gly Phe Trp Met Phe Asp
275 280 285
Ser Thr Ser Ala Thr Val Asn Gly Lys Thr Val Ala Gln Thr Gly Asn
290 295 300
Gln Ala Ile Ala Asp Thr Gly Thr Thr Leu Ala Leu Val Ser Asp Glu
305 310 315 320
Thr Cys Gln Ala Ile Tyr Asp Ala Ile Pro Gly Ser Thr Tyr Asp Ser
325 330 335
Glu Gln Gln Gly Tyr Thr Phe Pro Ser Asn Thr Ser Ala Asp Asp Leu
340 345 350
Pro Val Val Thr Phe Ala Val Gly Gly Lys Gln Phe Ala Val Gln Lys
355 360 365
Glu Asp Leu Gly Phe Ala Asp Ala Gly Asn Gly Met Val Tyr Gly Gly
370 375 380
Ile Gln Ser Arg Gly Ser Met Thr Phe Asp Ile Leu Gly Asp Thr Phe
385 390 395 400
Leu Lys Gly Ile Tyr Ala Ile Phe Asp Gln Gly Asn Thr Arg Phe Gly
405 410 415
Ala Val Gln Arg Thr Glu Ala Thr Gln Asn Thr Ala Ala Pro Pro Ala
420 425 430
<210> 30
<211> 677
<212> PRT
<213> 太瑞斯梭孢壳霉
<400> 30
Met Glu Ala Asp Phe Gln Leu Phe Ser Ser Leu Arg Tyr Asp Pro Val
1 5 10 15
Leu Leu Gln Val Pro Ala Ser Asn Leu Thr His Ala Gly Trp Asn Trp
20 25 30
Val Thr Ser Ser Pro Leu Tyr Met Leu Asp Tyr His Arg Asp Arg Met
35 40 45
Leu Gln Ala Ala Ala His Trp Gly Trp Gln Ala Ala Val Glu Val Leu
50 55 60
Asp Gly Asp Ala Gly Leu Lys Arg Leu Ala Gly Phe Val Met Asp Ser
65 70 75 80
Ile Gly Gly Asn Gln Pro Ser Pro Leu Arg Val Arg Val Ser Ile Ser
85 90 95
Lys Lys Gly Glu Met Ser Val Ile Ser Gly Pro Val Pro Glu Met Pro
100 105 110
Leu Ala Asn Leu Phe Pro Thr Arg Leu Pro Ala Pro Gly Glu Asp Pro
115 120 125
Glu Glu Ala His Ser Asn Gly Arg Ser Asp Gly Gln Pro Ser Lys Thr
130 135 140
Pro Glu Tyr Glu Ile Leu Val Asp Ser Leu Gly Ala Thr Trp Thr Ala
145 150 155 160
His Ser His Phe Lys Thr Thr Lys Arg Ala Val Tyr Asp Gly Ala Arg
165 170 175
Gln Arg Ala Gln Ile Ser Leu Ala Asp Lys Lys Glu Val Leu Ile Val
180 185 190
Asn Glu Ala Asp Gly Thr Val Met Glu Gly Ser Thr Thr Thr Pro Tyr
195 200 205
Phe Trp Arg Glu Gly Arg Trp Val Thr Pro Ala Val Asn Met Glu Asp
210 215 220
Lys Ser Glu Lys Gly Ala Gly Gly Gln Asn Gly Thr Ser Arg Arg Trp
225 230 235 240
Ala Leu Glu Lys Tyr Val Asn Thr Thr Pro Arg Pro Arg His Gln Asp
245 250 255
Asp Arg His His Arg His Leu His Lys Val Lys Ile Ile His Asn Gln
260 265 270
Asn Tyr Gln Arg His Gly Thr Lys Ser Tyr Val Ser Val Leu Asn Arg
275 280 285
Phe Gly Phe Gln Pro Thr Lys Pro Gly Pro Tyr Phe Gln Lys Phe Thr
290 295 300
Lys Pro Thr Asp Glu Ala Ala Arg Gly Lys Thr Gly Pro Gly Val Lys
305 310 315 320
Pro Gly His Val Trp Val Gly Leu Tyr Lys Lys Thr Lys Asp Asp Gln
325 330 335
Val Gly Glu Val Thr Ala Glu Asp Gln Gln Asn Asp Ala Glu Tyr Leu
340 345 350
Cys Glu Val Ser Ile Gly Thr Pro Pro Gln Lys Val Thr Leu Asp Phe
355 360 365
Asp Thr Gly Ser Ala Asp Leu Trp Val Asn His Lys Ser Phe Ala Pro
370 375 380
His Lys Ser Ser Thr Phe Lys Leu Ala Pro Asn Met Ser Trp Arg Ile
385 390 395 400
Gln Tyr Gly Asp Gly Ser Ser Ala Ser Gly Ser Val Gly Leu Asp Val
405 410 415
Leu Ser Ile Gly Gly Leu Thr Ile Lys Asn Gln Ala Ile Glu Ile Ala
420 425 430
Thr Thr Met Ser Asp Gln Phe Ser Gln Gly Ser Met Asp Gly Leu Leu
435 440 445
Gly Leu Ala Phe Ser Lys Ile Asn Thr Val Glu Thr Asn Gly Gln Pro
450 455 460
Asp Pro Gln Pro Thr Pro Val Glu Asn Met Ile Thr Gln Glu Asp Ile
465 470 475 480
Pro Glu Asp Ser Glu Leu Phe Thr Ser Ala Met Tyr Ser Thr Arg Asp
485 490 495
Gln Gly Glu Gln Ser Phe Tyr Thr Phe Gly Trp Ile Asp Gln Asp Leu
500 505 510
Val Lys Ala Ser Gly Gln Asp Ile Ser Trp Thr Asn Val Asp Thr Ser
515 520 525
Gln Gly Phe Trp Met Phe Pro Ser Glu Ser Ala Thr Val Asn Ser Lys
530 535 540
Lys Leu Ser Leu Ser Gly Asn Lys Ala Ile Ala Asp Thr Gly Thr Thr
545 550 555 560
Leu Ala Leu Val Ser Asp Glu Val Cys Glu Ala Leu Tyr Ser Gln Ile
565 570 575
Gln Gly Ser Tyr Tyr Ser Glu Gln Tyr Gln Gly Tyr Ile Ile Pro Lys
580 585 590
Ser Ile Thr Ala Asp Asp Leu Pro Asp Phe Ser Val Ala Val Gly Asp
595 600 605
Lys Glu Phe Val Ile Gln Lys Glu Asp Leu Leu Phe Ala Leu Ala Asp
610 615 620
Asp Asn Ser Trp Tyr Gly Ala Val Gln Ser Arg Gly Glu Asn Pro Phe
625 630 635 640
Asp Ile Leu Gly Asp Ala Phe Leu Lys Ser Ile Tyr Ala Ile Trp Asp
645 650 655
Gln Gly Asn Ser Arg Phe Gly Ala Val Pro Lys Ile Glu Lys Thr Gln
660 665 670
Asn Ile Lys Pro Glu
675
<210> 31
<211> 433
<212> PRT
<213> 核盘菌
<400> 31
Met Ala Thr Leu Gln Gly Leu Ser Arg Val Arg Leu Val Pro Asn Lys
1 5 10 15
Asn Tyr Lys Arg Ser Gly Ile Lys Ser Tyr Val Tyr Leu Leu Asn Lys
20 25 30
Trp Gly Phe Glu Pro Thr Leu Pro Gly Pro Tyr Phe Gln Met Asn Lys
35 40 45
Ala Thr Glu Arg Glu Ala Thr Tyr His Lys Phe Gly His Lys Ser His
50 55 60
His His Lys Arg His Leu Ala Lys Lys Thr Pro Thr Gly Glu Asn Gly
65 70 75 80
Glu Val Pro Ala Gln Asp Gln Gln Asn Asp Ser Glu Tyr Leu Cys Pro
85 90 95
Val Gln Ile Gly Thr Pro Ala Gln Thr Leu Met Leu Asp Phe Asp Thr
100 105 110
Gly Ser Ser Asp Leu Trp Val Trp Ser Thr Glu Leu Pro Lys Ser Thr
115 120 125
Thr Ser Lys Ala Thr Gly His Thr Ile Phe Asp Pro Thr Lys Ser Ser
130 135 140
Thr Phe Lys Ala Ser Lys Ser Ser Lys Trp Gln Ile Ser Tyr Gly Asp
145 150 155 160
Ser Ser Ser Ala Ser Gly Thr Val Gly Thr Asp Thr Val Thr Ile Gly
165 170 175
Gly Leu Ala Ile Lys Asn Gln Ala Val Glu Leu Ala Thr Lys Leu Ser
180 185 190
Ala Gln Phe Glu Asp Gly Ala Gly Asp Gly Leu Leu Gly Leu Ala Trp
195 200 205
Gly Ser Ile Asn Thr Val Thr Pro Glu Pro Val Ala Thr Pro Val Met
210 215 220
Asn Met Ile Thr Gln Glu Asp Ile Pro Lys Asp Ala Glu Leu Phe Thr
225 230 235 240
Val Asn Leu Gly Ser Trp Arg Asp Ala Glu Asp Pro Asp Lys Gly Glu
245 250 255
Ser Phe Tyr Thr Phe Gly Tyr Ile Asp Lys Asp Ile Val Gly Ser Gln
260 265 270
Glu Ile Ser Tyr Thr Pro Ile Asp Asn Ser Gln Gly Phe Trp Met Phe
275 280 285
Asp Ser Thr Ser Ala Thr Val Asn Gly Lys Thr Ile Thr Gln Asp Lys
290 295 300
Asn Gln Ala Ile Ala Asp Thr Gly Thr Thr Leu Ala Leu Val Ser Asp
305 310 315 320
Glu Thr Cys Gln Ala Ile Tyr Asp Ala Ile Pro Gly Ser Thr Tyr Asp
325 330 335
Gln Thr Gln Gln Gly Tyr Thr Phe Pro Ser Ser Thr Ser Ala Asp Asp
340 345 350
Leu Pro Val Val Thr Phe Ala Val Gly Gly Lys Gln Phe Ala Val Gln
355 360 365
Lys Glu Asp Leu Gly Phe Ala Asp Ala Gly Asn Gly Met Val Tyr Gly
370 375 380
Gly Ile Gln Ser Arg Gly Ser Met Thr Phe Asp Ile Leu Gly Asp Thr
385 390 395 400
Phe Leu Lys Gly Ile Tyr Ala Ile Phe Asp Gln Gly Asn Val Arg Phe
405 410 415
Gly Ala Val Gln Arg Asn Glu Pro Thr Gln Asn Thr Ser Ala Pro Pro
420 425 430
Ser
<210> 32
<211> 722
<212> PRT
<213> 球毛壳霉
<400> 32
Met Asp Asn Asp Phe Gln Leu Phe Thr Ser Leu Arg Tyr Asp Pro Ala
1 5 10 15
Leu Leu Gln Val Ser Val Arg Lys Leu Ser Tyr Gly Gly Trp Asn Ser
20 25 30
Val Asn Ser Ser Pro Leu Tyr Met Leu Asp Tyr His Arg Asp Arg Met
35 40 45
Leu Arg Ala Ala Thr His Trp Gly Trp Asn Ala Ala Val Asp Val Leu
50 55 60
Gln Gly Asp Ser Gly Leu Lys Arg Leu Val Glu Leu Val Glu Ser Ser
65 70 75 80
Ile Arg Glu Asp Gln Gln Ser Pro Leu Arg Val Arg Ile Ala Ile Thr
85 90 95
Lys Asp Gly Glu Leu Ser Ile Thr Ser Gly Pro Val Pro Glu Thr Thr
100 105 110
Leu Ala Asn Leu Phe Pro Glu Asn Leu Pro Pro Pro Glu Arg Ala Gly
115 120 125
His Asp Gly Glu Gly Arg Asp Ile Pro Ser Lys Ser Pro Gly Tyr Glu
130 135 140
Ile Leu Val Asp Thr Pro Ile Thr Thr Arg Ser Glu His Thr His Phe
145 150 155 160
Lys Thr Thr Lys Arg Ala Ala Tyr Asp Gly Ala Arg Gln Arg Ala Gln
165 170 175
Ile Ser Leu Phe Asp Leu Lys Glu Val Leu Ile Val Asn Glu Thr Asn
180 185 190
Gly Ala Val Met Glu Gly Ser Thr Thr Thr Pro Tyr Phe Trp Arg Asp
195 200 205
Gly Arg Trp Val Thr Pro Ala Val Ser Lys Glu Tyr Asn Leu Glu Asp
210 215 220
Gly Ser Gly Gly Gln Ser Gly Thr Ser Arg Arg Trp Ala Leu Glu Arg
225 230 235 240
Phe Val Asn Ile Ile Phe Ser Gly Arg Leu Glu Arg Pro Phe Pro Arg
245 250 255
Met Asp Asp Ala Leu Phe Gln Thr His Ala Asp Leu Arg Lys Arg Leu
260 265 270
Ala Leu His Lys Ile Lys Ala Ile Pro Asn Ser Asn Tyr Lys Arg His
275 280 285
Gly Thr Lys Ser Tyr Val Ser Val Leu Asn Arg Phe Gly Phe Gln Pro
290 295 300
Thr Gln Pro Gly Pro Tyr Tyr Gln Lys Phe Ile Lys Ser Asp Asp Ile
305 310 315 320
Pro Pro Lys Ala Ala Pro Gly Val Arg Pro Gly Arg Ile Trp Ala Gly
325 330 335
Leu Phe Lys Lys Val Glu Gly Lys Lys Glu Gly Gly Glu Val Thr Ala
340 345 350
Glu Asp Gln Gln Asn Asp Ala Glu Tyr Leu Cys Glu Val Thr Ile Gly
355 360 365
Thr Gly Val Gly Ser Asp Ile Gln Lys Val Met Leu Asp Phe Asp Thr
370 375 380
Gly Ser Ala Asp Leu Trp Val Ser His Lys Lys Phe Asp His Thr Lys
385 390 395 400
Ser Ser Ser Phe Gln Leu Ala Lys Asp Lys Thr Trp Lys Ile Gln Tyr
405 410 415
Gly Asp Gly Ser Ser Ala Ser Gly Thr Val Gly Thr Asp Val Leu Val
420 425 430
Leu Gly Gly Ile Ile Ile Lys Lys Gln Ala Ile Glu Ile Ala Asn Glu
435 440 445
Met Ser Ala Gln Phe Ser Glu Gly Thr Met Asp Gly Leu Leu Gly Leu
450 455 460
Ala Phe Ser Lys Ile Asn Thr Val Gln Ser Gly Gly Lys Pro Asp Pro
465 470 475 480
Gln Leu Thr Pro Val Asp Asn Met Ile Ala Gln Glu Asp Ile Pro Lys
485 490 495
Glu Ala Glu Leu Phe Thr Ser Ala Ile Tyr Ser Asn Arg Asp Glu Asp
500 505 510
Arg Arg Ser Phe Tyr Thr Phe Gly Trp Ile Asp Glu Asp Leu Val Lys
515 520 525
Ala Ser Gly Glu Glu Ile Ala Trp Thr Asn Ile Asp Lys Ser Glu Gly
530 535 540
Phe Trp Met Phe Ser Ser Glu His Ala Ser Val Asn Gly Glu Arg Ile
545 550 555 560
Thr Ile Glu Gly Asn Lys Ala Ile Ala Asp Thr Gly Thr Thr Leu Ala
565 570 575
Leu Val Thr Asp Lys Val Cys Ala Ala Leu Tyr Ser Lys Ile Pro Gly
580 585 590
Ala Glu Tyr Asn Ala Glu Tyr Gln Gly Trp Thr Ile Pro Gln Ser Ile
595 600 605
Pro Val Asp Glu Leu Pro Asp Phe Ser Val Ala Val Gly Gly Lys Glu
610 615 620
Phe Val Ile Gln Lys Glu Asp Leu Ile Phe Val Pro Ala Asp Lys Lys
625 630 635 640
Phe Phe Tyr Gly Gly Val Gln Ser Arg Gly Glu Asn Pro Phe Asp Ile
645 650 655
Leu Gly Asn Thr Phe Leu Lys Ser Ile Tyr Ala Ile Trp Asp Gln Gly
660 665 670
Asn Ser Arg Phe Gly Ala Val Arg Lys Ile Glu Pro Phe Gln His Pro
675 680 685
Ile Lys Tyr Asn Leu Gln Pro Leu Ser Ser Asp Glu Arg Thr Lys Leu
690 695 700
Gly Ile Lys Lys Gly Tyr Gly Asp Ile Asn Thr Ile Pro Phe Thr Glu
705 710 715 720
Ser Ala
<210> 33
<211> 481
<212> PRT
<213> 四孢链孢菌
<400> 33
Met Gly Ser Met Tyr Gln Val Gln Ser Lys Leu Arg Gln Asp Leu Gly
1 5 10 15
Leu His Lys Val Gln Ala Val Arg Lys Pro Gly Arg Glu Leu Asn Gly
20 25 30
Thr Lys Ala Tyr Val Ser Ala Met Ala Arg Tyr Gly Phe Asn Pro Thr
35 40 45
Glu Glu Ser Arg Phe Phe His Leu Lys Lys Thr Asp Leu Thr Lys Glu
50 55 60
Phe Gln Arg Arg Gly Tyr Thr Arg His Trp Glu Gln Leu Val Arg Thr
65 70 75 80
Pro Gln Glu Arg Pro Asp Asp Pro Gln Thr Asp Asn Glu Pro Ile Pro
85 90 95
Ala Glu Asp Gln Gln Tyr Asp Thr Gln Tyr Leu Cys Glu Ile Gly Ile
100 105 110
Gly Thr Pro Gln Gln Lys Val Lys Leu Asp Phe Asp Thr Gly Ser Ala
115 120 125
Asp Leu Trp Val Arg Cys Thr Asp Ser Ser Leu Leu His His Ala Asp
130 135 140
Lys Lys Phe Asp Pro Lys Lys Ser Asp Thr Phe Gln Glu Ser Lys Thr
145 150 155 160
Asp Gln Thr Trp Lys Ile Gln Tyr Gly Asp Gly Ser Thr Ala Ser Gly
165 170 175
Thr Val Gly Thr Asp Val Ile Thr Val Gly Gly Leu Gln Ile Lys Asn
180 185 190
Gln Ala Ile Glu Leu Ala Lys Lys Val Ser Ser Ala Phe Ser Ser Gly
195 200 205
Glu Ala Asp Gly Leu Leu Gly Leu Ala Phe Ser Thr Ile Asn Thr Ile
210 215 220
Glu Ser Asp Gly Lys Pro Asp Pro Gln Pro Thr Pro Val Glu Asn Met
225 230 235 240
Ile Ser Gln Asp Asp Ile Pro Lys Glu Ala Glu Leu Phe Thr Ser Ala
245 250 255
Phe Tyr Ser Ala Arg Asp Asp Lys Ser Glu Glu Lys Ser Phe Tyr Thr
260 265 270
Phe Gly Trp Val Asp Glu Asp Leu Val Lys Ala Ser Gly Lys Asp Ile
275 280 285
Thr Trp Thr Pro Ile Asp Asn Ser Glu Gly Phe Trp Lys Phe Pro Ser
290 295 300
Glu Ser Ala Thr Val Asp Gly Glu Asn Val Ser Val Ser Gly Asn Ala
305 310 315 320
Ala Ile Ala Asp Thr Gly Thr Thr Leu Ala Leu Val Ser Asp Thr Val
325 330 335
Cys Lys Ala Leu Tyr Ala Lys Ile Pro Gly Ser Lys Tyr Ser Tyr Arg
340 345 350
Tyr Gln Gly Tyr Leu Ile Pro Ser Thr Ile Thr Ala Asp Gln Leu Pro
355 360 365
Gln Leu Ser Val Ala Val Gly Gly Glu Gln Phe Val Ile Gln Lys Glu
370 375 380
Asp Leu Leu Leu Ala Pro Ala Asp Asp Asp His Trp Tyr Gly Gly Val
385 390 395 400
Gln Ser Arg Gly Thr Met Pro Phe Asp Ile Leu Gly Asp Thr Phe Leu
405 410 415
Lys Ser Ile Tyr Ala Ile Trp Asp Gln Gly Asn Asn Arg Phe Gly Ala
420 425 430
Val Pro Lys Ile Glu Val Asn Gln His Thr Val Phe Pro Asn Thr Glu
435 440 445
Ser Ser Pro Glu Ala Asn Ser Pro Glu Pro Ala Asp Lys Ile Gly Asp
450 455 460
Val Ser Pro Val Glu Gln Val Lys Gly Ala Val Lys Ser Leu Lys Leu
465 470 475 480
Leu
<210> 34
<211> 481
<212> PRT
<213> 粗糙链孢霉
<400> 34
Met Gly Ser Met Tyr Gln Val Gln Ser Lys Leu Arg Gln Asp Leu Gly
1 5 10 15
Leu His Lys Val Gln Ala Val Arg Lys Pro Gly Arg Glu Leu Asn Gly
20 25 30
Thr Lys Ala Tyr Val Ser Ala Met Ala Arg Tyr Gly Phe Asn Pro Thr
35 40 45
Glu Glu Ser Arg Phe Phe His Leu Lys Lys Thr Asp Leu Thr Lys Glu
50 55 60
Phe Gln Arg Arg Gly Tyr Ile Arg His Trp Glu Gln Leu Val Arg Thr
65 70 75 80
Pro Gln Glu Arg Pro Asp Asp Pro His Thr Asp Asn Glu Pro Val Pro
85 90 95
Ala Glu Asp Gln Gln Tyr Asp Thr Gln Tyr Leu Cys Glu Ile Gly Ile
100 105 110
Gly Thr Pro Gln Gln Lys Val Lys Leu Asp Phe Asp Thr Gly Ser Ala
115 120 125
Asp Leu Trp Val Arg Cys Thr Asp Ser Ser Leu Leu His His Ala Asp
130 135 140
Lys Lys Phe Asp Pro Lys Lys Ser Asp Thr Phe Gln Glu Ser Lys Thr
145 150 155 160
Asp Gln Thr Trp Lys Ile Gln Tyr Gly Asp Gly Ser Thr Ala Ser Gly
165 170 175
Thr Val Gly Thr Asp Val Ile Thr Val Gly Gly Leu Gln Ile Lys Asn
180 185 190
Gln Ala Ile Glu Leu Ala Lys Lys Val Ser Ser Ala Phe Ser Ser Gly
195 200 205
Glu Ala Asp Gly Leu Leu Gly Leu Ala Phe Ser Thr Ile Asn Thr Ile
210 215 220
Glu Ser Asp Gly Lys Pro Asp Pro Gln Pro Thr Pro Val Glu Asn Met
225 230 235 240
Ile Ser Gln Glu Asp Ile Pro Lys Glu Ala Glu Leu Phe Thr Ser Ala
245 250 255
Phe Tyr Ser Ala Arg Asp Asp Lys Ser Glu Glu Lys Ser Phe Tyr Thr
260 265 270
Phe Gly Trp Val Asp Glu Asp Leu Val Lys Ala Ser Gly Lys Asp Ile
275 280 285
Thr Trp Thr Pro Ile Asp Asn Ser Glu Gly Phe Trp Lys Phe Pro Ser
290 295 300
Glu Ser Ala Thr Val Asp Gly Asp Asn Val Ser Val Ser Gly Asn Thr
305 310 315 320
Ala Ile Ala Asp Thr Gly Thr Thr Leu Ala Leu Val Ser Asp Thr Val
325 330 335
Cys Lys Ala Leu Tyr Ala Lys Ile Pro Gly Ser Lys Tyr Ser Tyr Arg
340 345 350
Tyr Gln Gly Tyr Leu Ile Pro Ser Thr Ile Thr Ala Asp Gln Leu Pro
355 360 365
Gln Leu Ser Val Ala Val Gly Gly Glu Gln Phe Val Ile Gln Asn Glu
370 375 380
Asp Leu Leu Leu Ala Pro Ala Asp Asp Asp His Trp Tyr Gly Gly Val
385 390 395 400
Gln Ser Arg Gly Thr Met Pro Phe Asp Ile Leu Gly Asp Thr Phe Leu
405 410 415
Lys Ser Ile Tyr Ala Ile Trp Asp Gln Gly Asn Asn Arg Phe Gly Ala
420 425 430
Val Pro Lys Ile Glu Val Asn Gln His Thr Val Phe Pro Asp Thr Glu
435 440 445
Pro Ser Pro Glu Ala Ser Ser Pro Glu Pro Ala Asp Lys Val Gly Asp
450 455 460
Val Ser Pro Val Glu Gln Val Lys Gly Ala Val Lys Ser Leu Lys Val
465 470 475 480
Leu
<210> 35
<211> 459
<212> PRT
<213> 费希新萨托菌
<400> 35
Met Ser Ser Cys Arg Phe Ser Asn Thr Leu Lys Thr Ala Ala Ala Ser
1 5 10 15
Leu Cys Asn Tyr Leu His Thr Thr Thr Thr Ile Thr Met Ala Thr Lys
20 25 30
Ile Lys Leu Leu Pro Asn Pro Gln Tyr Arg Lys Ser Gly Thr Lys Ser
35 40 45
Tyr Val His Leu Met Arg Lys Tyr Arg Phe Thr Pro Thr Lys Gly Gly
50 55 60
Arg Tyr Phe Leu Ser Ser Ser Leu His Gln Thr Gly Arg Gln Tyr Thr
65 70 75 80
Asn Leu Pro Val Gly Gly Arg Ala Arg Ile Gln His Val Leu Arg Lys
85 90 95
Arg Ile Ala Asp Thr Asp Glu Thr Ser Glu Val Gly Ala Asp Asp Val
100 105 110
Gln Asn Asp Thr Met Tyr Leu Ala Pro Val Ser Ile Gly Thr Pro Ala
115 120 125
Gln Thr Val Asn Leu Glu Phe Asp Thr Gly Ser Thr Asp Leu Trp Val
130 135 140
Trp Ser Thr Asn Leu Pro Ser Ala Thr Ile Ser Gln His Lys Thr Gly
145 150 155 160
Thr Ile Phe Asp Pro Ser Lys Ser Ser Thr Phe Lys Glu Gln Ser Gly
165 170 175
Ser Thr Trp Lys Ile Ser Tyr Asp Asp Gly Ser Phe Ala Ser Gly Thr
180 185 190
Val Gly Thr Asp Thr Ile Ser Leu Gly Gly Leu Lys Val Glu Asn Gln
195 200 205
Ala Ile Lys Leu Ala Asp Thr Leu Ser Ala Gln Phe Glu Gln Gly Thr
210 215 220
Cys Asp Gly Leu Leu Gly Leu Ala Phe Ser Asn Ile Asn Thr Val Lys
225 230 235 240
Pro Gln Ala Val Arg Thr Pro Val Glu Asn Met Ile Ala Gln Glu Asp
245 250 255
Ile Pro Lys Ser Ala Lys Leu Phe Thr Ala Lys Leu Gly Thr Trp Lys
260 265 270
Asp Ala Asn Glu Pro Asp Glu Gly Glu Pro Phe Tyr Thr Phe Gly Phe
275 280 285
Ile Asp Gln Asp Thr Val Thr Ala Ser Gly Glu Glu Val Tyr Tyr Thr
290 295 300
Pro Ile Asp Asn Ser Gln Gly Phe Trp Leu Phe Asp Ser Ala Ser Ala
305 310 315 320
Thr Val Asn Gly Lys Thr Ile Ala Arg Ser Gly Asn Lys Ala Ile Ala
325 330 335
Asp Thr Gly Thr Thr Leu Ala Leu Val Asp Asp Glu Thr Cys Gln Ala
340 345 350
Ile Tyr Asp Ala Ile Pro Gly Ala His Tyr Asp Asp Glu Ser Gln Gly
355 360 365
Tyr Ile Phe Pro Ser Asn Thr Thr Glu Asp Lys Leu Pro Val Val Ser
370 375 380
Phe Ala Val Gly Asp Lys Gln Phe Val Val Gln Arg Glu Asp Leu Gly
385 390 395 400
Phe Ala Glu Ala Lys Ser Gly Tyr Val Tyr Gly Gly Ile Gln Ser Arg
405 410 415
Gly Ser Met Thr Met Asp Ile Leu Gly Asp Thr Phe Leu Lys Gly Ile
420 425 430
Tyr Ala Ile Phe Asp Val Gly Asn Leu Arg Phe Gly Ala Val Gln Arg
435 440 445
Lys Glu Leu His Gln Asn Leu Ser Val Pro Gln
450 455
<210> 36
<211> 431
<212> PRT
<213> 马尼弗青霉菌
<400> 36
Met Thr Ser Lys Val Arg Val Leu Leu Asn Pro Lys Tyr Lys Arg Asn
1 5 10 15
Gly Thr Lys Ser Tyr Val His Leu Leu Arg Lys Tyr Gly Phe Asp Thr
20 25 30
Thr Leu Asp Gly Pro Tyr Phe His Val Ser His Val His Gln Gln Gly
35 40 45
Lys Phe Thr Thr Ala Gly Gln Lys Thr Val Gly Gly Arg Val Arg Ile
50 55 60
His Arg Thr Leu Gln Lys Lys Val Asn Gly Gln Ala Gly Asp Val Pro
65 70 75 80
Ala Asp Asp Val Gln Asn Asp Thr Met Tyr Leu Ala Gln Val Gly Ile
85 90 95
Gly Thr Pro Ala Gln Thr Leu Lys Leu Asp Phe Asp Ser Gly Ser Ala
100 105 110
Asp Leu Trp Val Trp Ser Thr Glu Leu Pro Ser Ser Thr Gln Ser Ser
115 120 125
Gly Ser Gly His Thr Ile Phe Asp Pro Ser Lys Ser Ser Ser Trp Asn
130 135 140
Ser Ser Ser Gly Gln Lys Trp Gln Ile Ser Tyr Gly Asp Gly Ser Ser
145 150 155 160
Ala Ser Gly Asp Val Gly Thr Asp Thr Leu Asn Leu Gly Gly Ile Glu
165 170 175
Val Lys Asn Gln Ala Ile Glu Ile Ala Lys Asn Leu Ser Ser Gln Phe
180 185 190
Gln Gln Ser Glu Gly Asp Gly Leu Leu Gly Leu Ala Trp Gly Ser Ile
195 200 205
Asn Thr Val Gln Pro Gln Ala Val Gln Thr Pro Met Gln Asn Leu Ile
210 215 220
Ser Gln Asn Leu Leu Pro Ser Asn Ala Gln Leu Phe Thr Ala His Leu
225 230 235 240
Gly Glu Ala Thr Ala Lys Thr Asp Asn Thr Phe Tyr Thr Phe Gly Tyr
245 250 255
Ile Asp Gln Asp Thr Leu Asn Ala Thr Gly Asn Gln Ile Ala Tyr Ala
260 265 270
Asp Val Asp Asn Asn Gln Gly Phe Trp Thr Phe Gln Ser Thr Ser Ala
275 280 285
Thr Val Asn Gly Lys Thr Ile Asn Arg Ser Ala Asn Thr Ala Ile Ala
290 295 300
Asp Thr Gly Thr Thr Leu Ala Leu Ile Asp Asp Asn Thr Leu Lys Thr
305 310 315 320
Ile Tyr Asp Ala Ile Pro Gly Ser Lys Tyr Asp Ser Thr Gln Gln Gly
325 330 335
Tyr Ile Phe Pro Ser Asn Thr Pro Thr Asp Lys Leu Pro Thr Val Thr
340 345 350
Phe Ala Val Gly Gly Ser Gln Ile Ala Val His Lys Ser Ala Leu Gly
355 360 365
Phe Ala Asp Ala Gly Asn Gly Tyr Val Tyr Gly Gly Ile Gln Ser Arg
370 375 380
Gly Thr Asn Pro Phe Asp Ile Leu Gly Asp Thr Trp Leu Lys Gly Leu
385 390 395 400
Tyr Ala Val Phe Asp Val Gly Asn Thr Arg Phe Gly Ala Ala Ala Lys
405 410 415
Ala Asp Pro Asp Ala Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser
420 425 430
<210> 37
<211> 395
<212> PRT
<213> 里氏木霉
<400> 37
Met Lys Ser Ala Leu Leu Ala Ala Ala Ala Leu Val Gly Ser Ala Gln
1 5 10 15
Ala Gly Ile His Lys Met Lys Leu Gln Lys Val Ser Leu Glu Gln Gln
20 25 30
Leu Glu Gly Ser Ser Ile Glu Ala His Val Gln Gln Leu Gly Gln Lys
35 40 45
Tyr Met Gly Val Arg Pro Thr Ser Arg Ala Glu Val Met Phe Asn Asp
50 55 60
Lys Pro Pro Lys Val Gln Gly Gly His Pro Val Pro Val Thr Asn Phe
65 70 75 80
Met Asn Ala Gln Tyr Phe Ser Glu Ile Thr Ile Gly Thr Pro Pro Gln
85 90 95
Ser Phe Lys Val Val Leu Asp Thr Gly Ser Ser Asn Leu Trp Val Pro
100 105 110
Ser Gln Ser Cys Asn Ser Ile Ala Cys Phe Leu His Ser Thr Tyr Asp
115 120 125
Ser Ser Ser Ser Ser Thr Tyr Lys Pro Asn Gly Ser Asp Phe Glu Ile
130 135 140
His Tyr Gly Ser Gly Ser Leu Thr Gly Phe Ile Ser Asn Asp Val Val
145 150 155 160
Thr Ile Gly Asp Leu Lys Ile Lys Gly Gln Asp Phe Ala Glu Ala Thr
165 170 175
Ser Glu Pro Gly Leu Ala Phe Ala Phe Gly Arg Phe Asp Gly Ile Leu
180 185 190
Gly Leu Gly Tyr Asp Thr Ile Ser Val Asn Gly Ile Val Pro Pro Phe
195 200 205
Tyr Gln Met Val Asn Gln Lys Leu Ile Asp Glu Pro Val Phe Ala Phe
210 215 220
Tyr Leu Gly Ser Ser Asp Glu Gly Ser Glu Ala Val Phe Gly Gly Val
225 230 235 240
Asp Asp Ala His Tyr Glu Gly Lys Ile Glu Tyr Ile Pro Leu Arg Arg
245 250 255
Lys Ala Tyr Trp Glu Val Asp Leu Asp Ser Ile Ala Phe Gly Asp Glu
260 265 270
Val Ala Glu Leu Glu Asn Thr Gly Ala Ile Leu Asp Thr Gly Thr Ser
275 280 285
Leu Asn Val Leu Pro Ser Gly Leu Ala Glu Leu Leu Asn Ala Glu Ile
290 295 300
Gly Ala Lys Lys Gly Phe Gly Gly Gln Tyr Thr Val Asp Cys Ser Lys
305 310 315 320
Arg Asp Ser Leu Pro Asp Ile Thr Phe Ser Leu Ala Gly Ser Lys Tyr
325 330 335
Ser Leu Pro Ala Ser Asp Tyr Ile Ile Glu Met Ser Gly Asn Cys Ile
340 345 350
Ser Ser Phe Gln Gly Met Asp Phe Pro Glu Pro Val Gly Pro Leu Val
355 360 365
Ile Leu Gly Asp Ala Phe Leu Arg Arg Tyr Tyr Ser Val Tyr Asp Leu
370 375 380
Gly Arg Asp Ala Val Gly Leu Ala Lys Ala Lys
385 390 395
<210> 38
<211> 395
<212> PRT
<213> 绿木霉
<400> 38
Met Lys Ser Ala Leu Ile Ala Ala Ala Ala Leu Val Gly Ser Ala Gln
1 5 10 15
Ala Gly Val His Lys Met Lys Leu Gln Lys Val Ser Leu Glu Gln Gln
20 25 30
Leu Glu Gly Ser Thr Ile Glu Ser Gln Val Gln His Leu Gly Gln Lys
35 40 45
Tyr Met Gly Val Arg Pro Thr Ser Arg Ala Asp Val Met Phe Asn Asp
50 55 60
Lys Leu Pro Lys Ile Gln Gly Gly His Pro Val Pro Val Thr Asn Phe
65 70 75 80
Met Asn Ala Gln Tyr Phe Ser Glu Ile Thr Ile Gly Thr Pro Pro Gln
85 90 95
Thr Phe Lys Val Val Leu Asp Thr Gly Ser Ser Asn Leu Trp Val Pro
100 105 110
Ser Gln Ser Cys Asn Ser Ile Ala Cys Phe Leu His Ala Thr Tyr Asp
115 120 125
Ser Ser Ser Ser Ser Thr Tyr Lys Gln Asn Gly Ser Asp Phe Glu Ile
130 135 140
His Tyr Gly Ser Gly Ser Leu Thr Gly Phe Ile Ser Asn Asp Val Val
145 150 155 160
Thr Ile Gly Asp Leu Lys Ile Gln Lys Gln Asp Phe Ala Glu Ala Thr
165 170 175
Ser Glu Pro Gly Leu Ala Phe Ala Phe Gly Arg Phe Asp Gly Ile Leu
180 185 190
Gly Leu Gly Tyr Asp Thr Ile Ser Val Asn Gly Ile Ile Pro Pro Phe
195 200 205
Tyr Gln Met Val Asn Gln Lys Leu Leu Asp Glu Pro Val Phe Ala Phe
210 215 220
Tyr Leu Gly Ser Gly Asp Glu Gly Ser Glu Ala Val Phe Gly Gly Val
225 230 235 240
Asp Glu Ser His Tyr Ser Gly Lys Ile Glu Tyr Ile Pro Leu Arg Arg
245 250 255
Lys Ala Tyr Trp Glu Val Asp Leu Asp Ser Ile Ala Phe Gly Asp Glu
260 265 270
Val Ala Glu Leu Glu Asn Thr Gly Ala Ile Leu Asp Thr Gly Thr Ser
275 280 285
Leu Asn Val Leu Pro Ser Gly Leu Ala Glu Leu Leu Asn Ala Glu Ile
290 295 300
Gly Ala Lys Lys Gly Phe Gly Gly Gln Tyr Thr Val Asp Cys Ser Lys
305 310 315 320
Arg Asp Ser Leu Pro Asp Ile Thr Phe Ser Leu Ala Gly Ser Lys Tyr
325 330 335
Ser Leu Pro Ala Thr Asp Tyr Ile Ile Glu Met Ser Gly Asn Cys Ile
340 345 350
Ser Ser Phe Gln Gly Met Asp Phe Pro Glu Pro Val Gly Pro Leu Val
355 360 365
Ile Leu Gly Asp Ala Phe Leu Arg Arg Tyr Tyr Ser Val Tyr Asp Leu
370 375 380
Gly Lys Asn Ala Val Gly Leu Ala Lys Ala Lys
385 390 395
<210> 39
<211> 395
<212> PRT
<213> 深绿木霉
<400> 39
Met Lys Ser Ala Leu Ile Ala Ala Ala Ala Leu Val Gly Ser Ala Gln
1 5 10 15
Ala Gly Val His Lys Met Lys Leu Gln Lys Val Ser Leu Glu Gln Gln
20 25 30
Leu Glu Gly Ser Ser Ile Glu Ala Gln Val Gln Gln Leu Gly Gln Lys
35 40 45
Tyr Met Gly Val Arg Pro Thr Ser Arg Val Asp Val Met Phe Asn Asp
50 55 60
Asn Val Pro Lys Val Lys Gly Gly His Pro Val Pro Val Thr Asn Phe
65 70 75 80
Met Asn Ala Gln Tyr Phe Ser Glu Ile Thr Ile Gly Ser Pro Pro Gln
85 90 95
Thr Phe Lys Val Val Leu Asp Thr Gly Ser Ser Asn Leu Trp Val Pro
100 105 110
Ser Gln Ser Cys Asn Ser Ile Ala Cys Phe Leu His Ser Thr Tyr Asp
115 120 125
Ser Ser Ser Ser Ser Ser Tyr Lys Lys Asn Gly Ser Asp Phe Glu Ile
130 135 140
His Tyr Gly Ser Gly Ser Leu Thr Gly Phe Ile Ser Asn Asp Val Val
145 150 155 160
Thr Ile Gly Asp Leu Gln Ile Lys Gly Gln Asp Phe Ala Glu Ala Thr
165 170 175
Ser Glu Pro Gly Leu Ala Phe Ala Phe Gly Arg Phe Asp Gly Ile Leu
180 185 190
Gly Leu Gly Tyr Asp Thr Ile Ser Val Asn Gly Ile Val Pro Pro Phe
195 200 205
Tyr Gln Met Val Asn Gln Lys Leu Leu Asp Glu Pro Val Phe Ala Phe
210 215 220
Tyr Leu Gly Ser Gly Asp Glu Gly Ser Val Ala Thr Phe Gly Gly Val
225 230 235 240
Asp Glu Ser His Tyr Ser Gly Lys Ile Glu Tyr Ile Pro Leu Arg Arg
245 250 255
Lys Ala Tyr Trp Glu Val Asp Leu Asp Ser Ile Ala Phe Gly Asp Glu
260 265 270
Val Ala Glu Leu Glu Asn Thr Gly Ala Ile Leu Asp Thr Gly Thr Ser
275 280 285
Leu Asn Val Leu Pro Ser Gly Ile Ala Glu Leu Leu Asn Ala Glu Ile
290 295 300
Gly Ala Lys Lys Gly Tyr Gly Gly Gln Tyr Thr Ile Asp Cys Ala Lys
305 310 315 320
Arg Asp Ser Leu Pro Asp Ile Thr Phe Ser Leu Ala Gly Ser Lys Tyr
325 330 335
Ser Leu Pro Ala Ser Asp Tyr Ile Leu Glu Val Ser Gly Ser Cys Ile
340 345 350
Ser Thr Phe Gln Gly Met Asp Phe Pro Glu Pro Val Gly Pro Leu Val
355 360 365
Ile Leu Gly Asp Ala Phe Leu Arg Arg Tyr Tyr Ser Val Tyr Asp Leu
370 375 380
Gly Lys Gly Ala Val Gly Leu Ala Lys Ala Lys
385 390 395
<210> 40
<211> 395
<212> PRT
<213> 黄绿木霉
<400> 40
Met Lys Ser Ala Leu Ile Ala Ala Ala Ala Leu Val Gly Ser Ala Gln
1 5 10 15
Ala Gly Val His Lys Met Lys Leu Gln Lys Val Ser Leu Glu Gln Gln
20 25 30
Leu Glu Gly Ser Ser Ile Glu Ala Gln Val Gln Gln Leu Gly Gln Lys
35 40 45
Tyr Met Gly Val Arg Pro Thr Ser Arg Ala Asp Val Met Phe Asn Asp
50 55 60
Asn Leu Pro Lys Ile Lys Gly Gly His Pro Val Pro Val Thr Asn Phe
65 70 75 80
Met Asn Ala Gln Tyr Phe Ser Glu Ile Thr Ile Gly Ser Pro Pro Gln
85 90 95
Thr Phe Lys Val Val Leu Asp Thr Gly Ser Ser Asn Leu Trp Val Pro
100 105 110
Ser Gln Ser Cys Asn Ser Ile Ala Cys Phe Leu His Ser Thr Tyr Asp
115 120 125
Ser Ser Ser Ser Ser Thr Tyr Lys Lys Asn Gly Ser Asp Phe Glu Ile
130 135 140
His Tyr Gly Ser Gly Ser Leu Thr Gly Phe Ile Ser Asn Asp Val Val
145 150 155 160
Thr Ile Gly Asp Leu Lys Ile Lys Gly Gln Asp Phe Ala Glu Ala Thr
165 170 175
Ser Glu Pro Gly Leu Ala Phe Ala Phe Gly Arg Phe Asp Gly Ile Leu
180 185 190
Gly Leu Gly Tyr Asp Thr Ile Ser Val Asn Gly Ile Val Pro Pro Phe
195 200 205
Tyr Gln Met Val Asn Gln Lys Leu Leu Asp Glu Pro Val Phe Ala Phe
210 215 220
Tyr Leu Gly Asn Ser Asp Glu Gly Ser Val Ala Thr Phe Gly Gly Val
225 230 235 240
Asp Glu Ser His Phe Ser Gly Lys Ile Glu Tyr Ile Pro Leu Arg Arg
245 250 255
Lys Ala Tyr Trp Glu Val Asp Leu Asp Ser Ile Ala Phe Gly Asp Glu
260 265 270
Val Ala Glu Leu Glu Asn Thr Gly Ala Ile Leu Asp Thr Gly Thr Ser
275 280 285
Leu Asn Val Leu Pro Ser Gly Ile Ala Glu Leu Leu Asn Ala Glu Ile
290 295 300
Gly Ala Lys Lys Gly Tyr Gly Gly Gln Tyr Thr Ile Asp Cys Ala Lys
305 310 315 320
Arg Asp Ser Leu Pro Asp Ile Thr Phe Ser Leu Ala Gly Ser Lys Tyr
325 330 335
Ser Leu Pro Ala Ser Asp Tyr Ile Leu Glu Val Ser Gly Ser Cys Ile
340 345 350
Ser Thr Phe Gln Gly Met Asp Phe Pro Glu Pro Val Gly Pro Leu Val
355 360 365
Ile Leu Gly Asp Ala Phe Leu Arg Arg Tyr Tyr Ser Val Tyr Asp Leu
370 375 380
Gly Lys Gly Ala Val Gly Leu Ala Lys Ala Lys
385 390 395
<210> 41
<211> 398
<212> PRT
<213> 黑曲霉
<400> 41
Met Lys Ser Ala Ser Leu Leu Thr Ala Ser Val Leu Leu Gly Cys Ala
1 5 10 15
Ser Ala Glu Val His Lys Leu Lys Leu Asn Lys Val Pro Leu Glu Glu
20 25 30
Gln Leu Tyr Thr His Asn Ile Asp Ala His Val Arg Ala Leu Gly Gln
35 40 45
Lys Tyr Met Gly Ile Arg Pro Ser Ile His Lys Glu Leu Val Glu Glu
50 55 60
Asn Pro Ile Asn Asp Met Ser Arg His Asp Val Leu Val Asp Asn Phe
65 70 75 80
Leu Asn Ala Gln Tyr Phe Ser Glu Ile Glu Leu Gly Thr Pro Pro Gln
85 90 95
Lys Phe Lys Val Val Leu Asp Thr Gly Ser Ser Asn Leu Trp Val Pro
100 105 110
Ser Ser Glu Cys Ser Ser Ile Ala Cys Tyr Leu His Asn Lys Tyr Asp
115 120 125
Ser Ser Ala Ser Ser Thr Tyr His Lys Asn Gly Ser Glu Phe Ala Ile
130 135 140
Lys Tyr Gly Ser Gly Ser Leu Ser Gly Phe Ile Ser Gln Asp Thr Leu
145 150 155 160
Lys Ile Gly Asp Leu Lys Val Lys Gly Gln Asp Phe Ala Glu Ala Thr
165 170 175
Asn Glu Pro Gly Leu Ala Phe Ala Phe Gly Arg Phe Asp Gly Ile Leu
180 185 190
Gly Leu Gly Tyr Asp Thr Ile Ser Val Asn Lys Ile Val Pro Pro Phe
195 200 205
Tyr Asn Met Leu Asp Gln Gly Leu Leu Asp Glu Pro Val Phe Ala Phe
210 215 220
Tyr Leu Gly Asp Thr Asn Lys Glu Gly Asp Glu Ser Val Ala Thr Phe
225 230 235 240
Gly Gly Val Asp Lys Asp His Tyr Thr Gly Glu Leu Ile Lys Ile Pro
245 250 255
Leu Arg Arg Lys Ala Tyr Trp Glu Val Glu Leu Asp Ala Ile Ala Leu
260 265 270
Gly Asp Asp Val Ala Glu Met Glu Asn Thr Gly Val Ile Leu Asp Thr
275 280 285
Gly Thr Ser Leu Ile Ala Leu Pro Ala Asp Leu Ala Glu Met Ile Asn
290 295 300
Ala Gln Ile Gly Ala Lys Lys Gly Trp Thr Gly Gln Tyr Thr Val Asp
305 310 315 320
Cys Asp Lys Arg Ser Ser Leu Pro Asp Val Thr Phe Thr Leu Ala Gly
325 330 335
His Asn Phe Thr Ile Ser Ser Tyr Asp Tyr Thr Leu Glu Val Gln Gly
340 345 350
Ser Cys Val Ser Ala Phe Met Gly Met Asp Phe Pro Glu Pro Val Gly
355 360 365
Pro Leu Ala Ile Leu Gly Asp Ala Phe Leu Arg Lys Trp Tyr Ser Val
370 375 380
Tyr Asp Leu Gly Asn Ser Ala Val Gly Leu Ala Lys Ala Lys
385 390 395
<210> 42
<211> 398
<212> PRT
<213> 烟曲霉
<400> 42
Met Lys Ser Thr Ser Leu Leu Thr Ala Ser Val Leu Leu Gly Ser Ala
1 5 10 15
Ser Ala Ala Val His Lys Leu Lys Leu Asn Lys Val Pro Leu Asp Glu
20 25 30
Gln Leu Tyr Thr His Asn Ile Asp Ala His Val Arg Ala Leu Gly Gln
35 40 45
Lys Tyr Met Gly Ile Arg Pro Asn Val His Gln Glu Leu Leu Glu Glu
50 55 60
Asn Ser Leu Asn Asp Met Ser Arg His Asp Val Leu Val Asp Asn Phe
65 70 75 80
Leu Asn Ala Gln Tyr Phe Ser Glu Ile Ser Leu Gly Thr Pro Pro Gln
85 90 95
Lys Phe Lys Val Val Leu Asp Thr Gly Ser Ser Asn Leu Trp Val Pro
100 105 110
Gly Ser Asp Cys Ser Ser Ile Ala Cys Phe Leu His Asn Lys Tyr Asp
115 120 125
Ser Ser Ala Ser Ser Thr Tyr Lys Ala Asn Gly Thr Glu Phe Ala Ile
130 135 140
Lys Tyr Gly Ser Gly Glu Leu Ser Gly Phe Val Ser Gln Asp Thr Leu
145 150 155 160
Gln Ile Gly Asp Leu Lys Val Val Lys Gln Asp Phe Ala Glu Ala Thr
165 170 175
Asn Glu Pro Gly Leu Ala Phe Ala Phe Gly Arg Phe Asp Gly Ile Leu
180 185 190
Gly Leu Gly Tyr Asp Thr Ile Ser Val Asn Lys Ile Val Pro Pro Phe
195 200 205
Tyr Asn Met Leu Asp Gln Gly Leu Leu Asp Glu Pro Val Phe Ala Phe
210 215 220
Tyr Leu Gly Asp Thr Asn Lys Glu Gly Asp Asn Ser Glu Ala Ser Phe
225 230 235 240
Gly Gly Val Asp Lys Asn His Tyr Thr Gly Glu Leu Thr Lys Ile Pro
245 250 255
Leu Arg Arg Lys Ala Tyr Trp Glu Val Asp Phe Asp Ala Ile Ala Leu
260 265 270
Gly Asp Asn Val Ala Glu Leu Glu Asn Thr Gly Ile Ile Leu Asp Thr
275 280 285
Gly Thr Ser Leu Ile Ala Leu Pro Ser Thr Leu Ala Asp Leu Leu Asn
290 295 300
Lys Glu Ile Gly Ala Lys Lys Gly Phe Thr Gly Gln Tyr Ser Ile Glu
305 310 315 320
Cys Asp Lys Arg Asp Ser Leu Pro Asp Leu Thr Phe Thr Leu Ala Gly
325 330 335
His Asn Phe Thr Ile Gly Pro Tyr Asp Tyr Thr Leu Glu Val Gln Gly
340 345 350
Ser Cys Ile Ser Ser Phe Met Gly Met Asp Phe Pro Glu Pro Val Gly
355 360 365
Pro Leu Ala Ile Leu Gly Asp Ala Phe Leu Arg Lys Trp Tyr Ser Val
370 375 380
Tyr Asp Leu Gly Asn Asn Ala Val Gly Leu Ala Lys Ala Lys
385 390 395
<210> 43
<211> 398
<212> PRT
<213> 棒曲霉
<400> 43
Met Lys Thr Val Ser Leu Leu Thr Ala Ser Ala Leu Leu Gly Cys Ala
1 5 10 15
Ser Ala Glu Val His Lys Leu Lys Leu Asn Lys Val Pro Leu Glu Glu
20 25 30
Gln Leu Tyr Thr His Asn Ile Asp Ala His Val Arg Ala Leu Gly Gln
35 40 45
Lys Tyr Met Gly Ile Arg Pro Asn Ile His Lys Glu Leu Leu Glu Glu
50 55 60
Asn Ser Phe Asn Asp Met Ser Arg His Asp Val Leu Val Asp Asn Phe
65 70 75 80
Leu Asn Ala Gln Tyr Phe Ser Glu Ile Glu Leu Gly Thr Pro Pro Gln
85 90 95
Lys Phe Lys Val Val Leu Asp Thr Gly Ser Ser Asn Leu Trp Val Pro
100 105 110
Ser Ser Glu Cys Gly Ser Ile Ala Cys Tyr Leu His Thr Lys Tyr Asp
115 120 125
Ser Ser Ala Ser Ser Thr Tyr Lys Lys Asn Gly Thr Glu Phe Ala Ile
130 135 140
Arg Tyr Gly Ser Gly Glu Leu Ser Gly Phe Val Ser Gln Asp Asn Leu
145 150 155 160
Lys Ile Gly Asp Leu Lys Ile Glu Lys Gln Asp Phe Ala Glu Ala Thr
165 170 175
Asn Glu Pro Gly Leu Ala Phe Ala Phe Gly Arg Phe Asp Gly Ile Leu
180 185 190
Gly Leu Gly Tyr Asp Thr Ile Ser Val Asn Lys Ile Val Pro Pro Phe
195 200 205
Tyr Asn Met Leu Asn Gln Gly Leu Leu Asp Glu Pro Val Phe Ala Phe
210 215 220
Tyr Leu Gly Asp Ala Asn Lys Glu Gly Asp Ser Ser Val Ala Thr Phe
225 230 235 240
Gly Gly Ile Asp Lys Asp His Phe Thr Gly Glu Leu Thr Lys Ile Pro
245 250 255
Leu Arg Arg Lys Ala Tyr Trp Glu Val Asp Leu Asp Ala Ile Ala Leu
260 265 270
Gly Asp Asn Val Ala Glu Leu Asp Asn Thr Gly Val Ile Leu Asp Thr
275 280 285
Gly Thr Ser Leu Ile Ala Leu Pro Ser Thr Leu Ala Asp Leu Leu Asn
290 295 300
Lys Glu Ile Gly Ala Lys Lys Gly Phe Thr Gly Gln Tyr Ser Val Glu
305 310 315 320
Cys Asp Lys Arg Asp Ser Leu Pro Asp Leu Thr Phe Thr Leu Ser Gly
325 330 335
His Asn Phe Thr Ile Gly Pro Tyr Asp Tyr Thr Leu Glu Val Gln Gly
340 345 350
Ser Cys Ile Ser Ser Phe Met Gly Met Asp Phe Pro Glu Pro Val Gly
355 360 365
Pro Leu Ala Ile Leu Gly Asp Ala Phe Leu Arg Lys Tyr Tyr Ser Val
370 375 380
Tyr Asp Leu Gly Asn His Ala Val Gly Leu Ala Lys Ala Lys
385 390 395
<210> 44
<211> 396
<212> PRT
<213> Nectria haematococca
<400> 44
Met Lys Ser Ala Leu Leu Ala Ala Ala Ala Leu Leu Gly Ser Ala Gln
1 5 10 15
Ala Gly Val His Lys Met Lys Ile Gln Lys Val Pro Leu Ala Glu Gln
20 25 30
Leu Ala Thr Thr Ser Ile Glu Thr His Ile Gln Asn Leu Gly Gln Lys
35 40 45
Tyr Leu Gly Ser Ala Arg Pro Lys Asn Gln Ala Asp Tyr Ala Phe Ser
50 55 60
Thr Glu Ala Ile Asn Val Glu Gly Gly His Pro Val Pro Ile Ser Asn
65 70 75 80
Phe Met Asn Ala Gln Tyr Phe Ser Glu Ile Thr Ile Gly Asn Pro Pro
85 90 95
Gln Ser Phe Lys Val Val Leu Asp Thr Gly Ser Ser Asn Leu Trp Val
100 105 110
Pro Ser Gln Glu Cys Gly Ser Ile Ala Cys Tyr Leu His Ser Lys Tyr
115 120 125
Asp Ser Ser Ala Ser Ser Thr Tyr Lys Gln Asn Gly Ser Glu Phe Glu
130 135 140
Ile His Tyr Gly Ser Gly Ser Leu Ser Gly Phe Ile Ser Asn Asp Asp
145 150 155 160
Val Ser Ile Gly Asp Leu Lys Ile Lys Gly Gln Asp Phe Ala Glu Ala
165 170 175
Thr Lys Glu Pro Gly Leu Ala Phe Ala Phe Gly Arg Phe Asp Gly Ile
180 185 190
Leu Gly Leu Gly Tyr Asp Thr Ile Ser Val Asn His Ile Val Pro Pro
195 200 205
Phe Tyr Gln Met Val Asn Gln Lys Leu Leu Asp Asp Pro Val Phe Ala
210 215 220
Phe Tyr Leu Ala Asp Gln Glu Gly Glu Ser Glu Val Val Phe Gly Gly
225 230 235 240
Val Asp Lys Ser His Tyr Glu Gly Asp Ile Glu Tyr Ile Pro Leu Arg
245 250 255
Arg Lys Ala Tyr Trp Glu Val Asp Leu Asp Ala Ile Ala Leu Gly Asp
260 265 270
Glu Val Ala Glu Gln Glu Asn Thr Gly Ala Ile Leu Asp Thr Gly Thr
275 280 285
Ser Leu Asn Val Leu Pro Ser Ala Leu Ala Glu Leu Leu Asn Lys Glu
290 295 300
Ile Gly Ala Lys Lys Gly Tyr Asn Gly Gln Tyr Thr Val Glu Cys Asp
305 310 315 320
Lys Arg Gln Thr Leu Pro Asp Ile Thr Phe Thr Leu Ala Gly Ser Asn
325 330 335
Tyr Ser Leu Pro Ala Thr Asp Tyr Ile Leu Glu Val Ser Gly Ser Cys
340 345 350
Ile Ser Thr Phe Gln Gly Met Asp Phe Pro Glu Pro Val Gly Pro Leu
355 360 365
Val Ile Leu Gly Asp Ala Phe Leu Arg Arg Tyr Tyr Ser Val Tyr Asp
370 375 380
Leu Gly Lys Asn Ala Val Gly Leu Ala Arg Ser Lys
385 390 395
<210> 45
<211> 399
<212> PRT
<213> Glomerella graminicola
<400> 45
Met Lys Ser Thr Leu Leu Thr Ala Ala Val Leu Leu Gly Ala Ala Gln
1 5 10 15
Ala Glu Val His Lys Leu Lys Leu Lys Lys Val Pro Leu Glu Glu Gln
20 25 30
Leu Asn Ala Val Pro Ile Glu His Gln Val Arg Gln Leu Gly Gln Lys
35 40 45
Tyr Met Gly Thr Arg Pro Asn Asn His Ala Asp Ala Met Phe Asn Gln
50 55 60
Lys Pro Ile Gln Thr Asp Gly Glu His Pro Val Pro Val Ser Asn Phe
65 70 75 80
Met Asn Ala Gln Tyr Phe Ser Glu Ile Gln Ile Gly Thr Pro Pro Gln
85 90 95
Thr Phe Lys Val Val Leu Asp Thr Gly Ser Ser Asn Leu Trp Val Pro
100 105 110
Ser Gln Gln Cys Gly Ser Ile Ala Cys Tyr Leu His Thr Lys Tyr Asp
115 120 125
Ser Ser Ala Ser Ser Thr Tyr Lys Ser Asn Gly Ser Ser Phe Glu Ile
130 135 140
His Tyr Gly Ser Gly Ser Leu Thr Gly Phe Val Ser Gln Asp Asp Val
145 150 155 160
Ser Ile Gly Asp Leu Lys Ile Lys Lys Gln Asp Phe Ala Glu Ala Thr
165 170 175
Ser Glu Pro Gly Leu Ala Phe Ala Phe Gly Arg Phe Asp Gly Ile Leu
180 185 190
Gly Leu Gly Tyr Asp Thr Ile Ser Val Asn Lys Ile Val Pro Pro Phe
195 200 205
Tyr Asn Leu Val Asn Gln Lys Ala Ile Asp Glu Pro Val Phe Ala Phe
210 215 220
Tyr Leu Gly Asp Thr Asn Glu Glu Gly Asp Glu Ser Glu Ala Thr Phe
225 230 235 240
Gly Gly Leu Asp Glu Ser His Tyr Glu Gly Lys Val Thr Tyr Ile Pro
245 250 255
Leu Arg Arg Lys Ala Tyr Trp Glu Val Asp Leu Asp Ala Ile Ser Leu
260 265 270
Gly Asp Glu Thr Ala Asp Leu Glu Gly His Gly Ala Ile Leu Asp Thr
275 280 285
Gly Thr Ser Leu Asn Val Leu Pro Ser Ala Leu Ala Glu Leu Leu Asn
290 295 300
Lys Glu Ile Gly Ala Lys Lys Gly Tyr Asn Gly Gln Tyr Ser Val Glu
305 310 315 320
Cys Ser Lys Arg Asp Glu Leu Pro Asp Ile Thr Phe Thr Leu Ala Gly
325 330 335
Tyr Asn Phe Ser Ile Ser Ala Tyr Asp Tyr Val Leu Glu Val Ser Gly
340 345 350
Ser Cys Ile Ser Thr Phe Gln Gly Met Asp Phe Pro Glu Pro Val Gly
355 360 365
Pro Leu Val Ile Leu Gly Asp Ala Phe Leu Arg Arg Trp Tyr Ser Val
370 375 380
Tyr Asp Leu Gly Lys Asn Ala Val Gly Leu Ala Lys Ala Lys Lys
385 390 395
<210> 46
<211> 395
<212> PRT
<213> 蛹虫草
<400> 46
Met Lys Ser Ala Leu Ile Ala Ala Ala Val Leu Ala Gly Ser Ala His
1 5 10 15
Ala Gly Ile His Lys Met Lys Leu Gln Lys Ile Pro Leu Ala Glu Gln
20 25 30
Leu Val Gly Ala Ser Phe Glu Ala Gln Ala Gln Gln Leu Gly Gln Lys
35 40 45
Tyr Leu Gly Ala Arg Pro Ala Ser Arg Ala Asp Ile Ile Phe Asn Ala
50 55 60
Lys Val Ala Glu Ser Lys Asn Gly His Leu Val Pro Val Ser Asn Phe
65 70 75 80
Ala Asn Ala Gln Tyr Phe Ser Glu Ile Thr Ile Gly Thr Pro Pro Gln
85 90 95
Thr Phe Lys Val Val Leu Asp Thr Gly Ser Ser Asn Leu Trp Val Pro
100 105 110
Ser Gln Ser Cys Ser Ser Ile Ala Cys Phe Leu His Ser Thr Tyr Asp
115 120 125
Ser Ser Ser Ser Ser Thr Tyr Lys Lys Asn Gly Ser Asp Phe Glu Ile
130 135 140
His Tyr Gly Ser Gly Ser Leu Thr Gly Tyr Val Ser Asn Asp Val Val
145 150 155 160
Arg Ile Gly Asp Leu Thr Ile Lys Asn Thr Asp Phe Ala Glu Ala Thr
165 170 175
Asn Glu Pro Gly Leu Ala Phe Ala Phe Gly Arg Phe Asp Gly Ile Leu
180 185 190
Gly Leu Gly Tyr Asp Thr Ile Ser Val Asn His Met Val Pro Pro Phe
195 200 205
Tyr Gln Met Ile Lys Gln Lys Leu Leu Asp Glu Pro Val Phe Ala Phe
210 215 220
Tyr Leu Gly Ser Glu Glu Glu Gly Ser Glu Ala Val Phe Gly Gly Val
225 230 235 240
Asp Lys Asn His Tyr Glu Gly Lys Ile Glu Tyr Leu Pro Leu Arg Arg
245 250 255
Lys Ala Tyr Trp Glu Val Asp Phe Asp Ala Ile Ala Phe Gly Lys Glu
260 265 270
Val Ala Glu Leu Glu Asn Thr Gly Val Ile Leu Asp Thr Gly Thr Ser
275 280 285
Leu Asn Thr Leu Pro Ser Asp Leu Ala Glu Leu Leu Asn Lys Glu Ile
290 295 300
Gly Ala Lys Lys Gly Phe Gly Gly Gln Tyr Thr Ile Asp Cys Ala Ala
305 310 315 320
Arg Asp Lys Leu Pro Asp Ile Thr Phe Thr Leu Ala Gly Ser Asn Tyr
325 330 335
Thr Leu Pro Ala Thr Asp Tyr Ile Leu Glu Leu Gly Gly Ser Cys Val
340 345 350
Ser Thr Phe Thr Pro Leu Asp Met Pro Pro Pro Ala Gly Pro Leu Ala
355 360 365
Ile Leu Gly Asp Ala Phe Leu Arg Arg Tyr Tyr Ser Val Tyr Asp Leu
370 375 380
Asn Lys Asn Ala Val Gly Leu Ala Arg Ala Lys
385 390 395
<210> 47
<211> 396
<212> PRT
<213> 玉米赤霉
<400> 47
Met Lys Ser Ala Leu Leu Thr Ala Ala Ala Leu Leu Gly Ser Ala Gln
1 5 10 15
Ala Gly Val His Lys Met Lys Leu Asn Lys Val Pro Leu Ala Asp Gln
20 25 30
Leu Ala Met Asn Ser Val Glu Asp His Leu Gln Ser Leu Gly Gln Lys
35 40 45
Tyr Met Gly Ala Ala Arg Pro Lys Asn Ala Ala Asp Tyr Ala Phe Ala
50 55 60
Thr Asn Val Pro Ser Val Glu Gly Gly His Pro Val Pro Val Ser Asn
65 70 75 80
Phe Met Asn Ala Gln Tyr Phe Ser Glu Ile Thr Ile Gly Thr Pro Pro
85 90 95
Gln Ser Phe Lys Val Val Leu Asp Thr Gly Ser Ser Asn Leu Trp Val
100 105 110
Pro Ser Gln Glu Cys Gly Ser Ile Ala Cys Tyr Leu His Ser Lys Tyr
115 120 125
Asp Ser Ser Ala Ser Ser Thr Tyr Lys Lys Asn Gly Ser Glu Phe Glu
130 135 140
Ile His Tyr Gly Ser Gly Ser Leu Ser Gly Phe Val Ser Asn Asp Val
145 150 155 160
Val Ser Ile Gly Asp Leu Lys Ile Lys Asp Gln Asp Phe Ala Glu Ala
165 170 175
Thr Lys Glu Pro Gly Leu Ala Phe Ala Phe Gly Arg Phe Asp Gly Ile
180 185 190
Leu Gly Leu Gly Tyr Asp Arg Ile Ala Val Asn Gly Met Val Pro Pro
195 200 205
Phe Tyr Gln Met Val Asn Gln Lys Leu Leu Asp Glu Pro Val Phe Ala
210 215 220
Phe Tyr Leu Asp Gly Gln Glu Gly Gln Ser Glu Ala Thr Phe Gly Gly
225 230 235 240
Val Asp Lys Ser Lys Tyr Thr Gly Asp Leu Glu Tyr Ile Pro Leu Arg
245 250 255
Arg Lys Ala Tyr Trp Glu Val Asp Leu Asp Ala Ile Ala Phe Gly Asp
260 265 270
Glu Val Ala Glu Gln Glu Asn Thr Gly Ala Ile Leu Asp Thr Gly Thr
275 280 285
Ser Leu Asn Val Leu Pro Ser Ala Leu Ala Glu Leu Leu Asn Lys Glu
290 295 300
Ile Gly Ala Lys Lys Gly Tyr Asn Gly Gln Tyr Thr Ile Glu Cys Asp
305 310 315 320
Lys Val Ser Ser Leu Pro Asp Ile Thr Phe Thr Leu Ala Gly Ser Asn
325 330 335
Tyr Ser Leu Pro Ser Thr Asp Tyr Ile Leu Glu Val Gln Gly Ser Cys
340 345 350
Ile Ser Thr Phe Gln Gly Met Asp Phe Pro Glu Pro Val Gly Pro Leu
355 360 365
Val Ile Leu Gly Asp Ala Phe Leu Arg Arg Tyr Tyr Ser Val Tyr Asp
370 375 380
Leu Gly Lys Asn Ala Val Gly Leu Ala Arg Ala Lys
385 390 395
<210> 48
<211> 395
<212> PRT
<213> 金龟子绿僵菌
<400> 48
Met Lys Ser Ala Leu Ile Ala Ala Ala Ala Leu Ala Gly Thr Ala His
1 5 10 15
Ala Gly Val His Lys Met Lys Leu Gln Lys Ile Ser Leu Glu Glu Gln
20 25 30
Leu Ala Gly Ala Ser Ile Glu Gln His Val Arg Ala Leu Gly Gln Lys
35 40 45
Tyr Leu Gly Ala Arg Pro Ala Ser Arg Ala Ser Val Met Phe Asn Thr
50 55 60
Lys Ala Pro Gln Val Ala Glu Gly His Pro Val Pro Val Ser Asn Phe
65 70 75 80
Met Asn Ala Gln Tyr Phe Ser Glu Ile Thr Val Gly Thr Pro Pro Gln
85 90 95
Thr Phe Lys Val Val Leu Asp Thr Gly Ser Ser Asn Leu Trp Val Pro
100 105 110
Ser Gln Ser Cys Ser Ser Ile Ala Cys Tyr Leu His Ser Thr Tyr Asp
115 120 125
Ser Ser Ser Ser Ser Thr Tyr Lys Lys Asn Gly Ser Ser Phe Glu Ile
130 135 140
Arg Tyr Gly Ser Gly Ser Leu Ser Gly Phe Val Ser Gln Asp Val Val
145 150 155 160
Thr Ile Gly Asp Leu Lys Ile Lys Asp Gln Asp Phe Ala Glu Ala Thr
165 170 175
Ser Glu Pro Gly Leu Ala Phe Ala Phe Gly Lys Phe Asp Gly Ile Leu
180 185 190
Gly Leu Gly Tyr Asp Thr Leu Ser Val Asn Lys Ile Val Pro Pro Phe
195 200 205
Tyr Gln Met Ile Asn Gln Lys Leu Leu Asp Glu Pro Val Phe Ala Phe
210 215 220
Tyr Leu Gly Ser Ser Glu Glu Gly Ser Glu Ala Val Phe Gly Gly Ile
225 230 235 240
Asp Lys Asp His Tyr Thr Gly Lys Ile Glu Tyr Ile Pro Leu Arg Arg
245 250 255
Lys Ala Tyr Trp Glu Val Asp Ile His Ser Ile Ala Phe Gly Asp Asp
260 265 270
Val Ala Glu Leu Asp Arg Thr Gly Ala Ile Leu Asp Thr Gly Thr Ser
275 280 285
Leu Asn Val Leu Pro Ser Thr Leu Ala Glu Leu Leu Asn Lys Glu Ile
290 295 300
Gly Ala Lys Lys Ser Trp Asn Gly Gln Tyr Thr Val Asp Cys Ala Gln
305 310 315 320
Ile Lys Ser Leu Pro Asp Ile Val Phe Asn Leu Ala Gly Ser Asn Tyr
325 330 335
Ser Leu Pro Ala Ser Asp Tyr Ile Leu Glu Leu Gln Gly Thr Cys Ile
340 345 350
Ser Thr Phe Gln Gly Met Asp Ile Pro Glu Pro Ala Gly Pro Leu Ile
355 360 365
Ile Leu Gly Asp Ala Phe Leu Arg Arg Tyr Tyr Ser Ile Tyr Asp Leu
370 375 380
Gly Arg Asn Ala Val Gly Leu Ala Arg Ser Lys
385 390 395
<210> 49
<211> 396
<212> PRT
<213> 尖孢镰刀菌
<400> 49
Met Lys Gly Ala Leu Leu Thr Ala Ala Ala Leu Leu Gly Ser Ala Gln
1 5 10 15
Ala Gly Val His Lys Met Lys Leu Asn Lys Val Pro Leu Ala Asp Gln
20 25 30
Leu Ala Thr Asn Ser Val Glu Asp His Leu Gln Ser Leu Gly Gln Lys
35 40 45
Tyr Leu Gly Ala Ser Arg Pro Lys Asn Ala Ala Asp Tyr Ala Phe Ala
50 55 60
Thr Asn Thr Val Asn Val Glu Gly Gly His Pro Val Pro Val Ser Asn
65 70 75 80
Phe Met Asn Ala Gln Tyr Phe Ser Glu Ile Thr Ile Gly Thr Pro Pro
85 90 95
Gln Ser Phe Lys Val Val Leu Asp Thr Gly Ser Ser Asn Leu Trp Val
100 105 110
Pro Ser Gln Gln Cys Gly Ser Ile Ala Cys Tyr Leu His Ser Lys Tyr
115 120 125
Asp Ser Ser Ala Ser Ser Thr Tyr Lys Glu Asn Gly Thr Glu Phe Glu
130 135 140
Ile His Tyr Gly Ser Gly Ser Leu Ser Gly Phe Val Ser Asn Asp Val
145 150 155 160
Val Ser Ile Gly Asp Leu Glu Ile Lys Asp Gln Asp Phe Ala Glu Ala
165 170 175
Thr Lys Glu Pro Gly Leu Ala Phe Ala Phe Gly Arg Phe Asp Gly Ile
180 185 190
Leu Gly Leu Gly Tyr Asp Arg Ile Ala Val Asn Gly Met Val Pro Pro
195 200 205
Phe Tyr Gln Met Val Asn Gln Lys Leu Leu Asp Glu Pro Val Phe Ala
210 215 220
Phe Tyr Leu Asp Asp Gln Glu Gly Glu Ser Glu Ala Thr Phe Gly Gly
225 230 235 240
Ile Asp Lys Ser Lys Phe Thr Gly Asp Ile Glu Tyr Ile Pro Leu Arg
245 250 255
Arg Lys Ala Tyr Trp Glu Val Asp Leu Glu Ala Ile Ala Phe Gly Asp
260 265 270
Glu Val Ala Glu Gln Glu Asn Thr Gly Ala Ile Leu Asp Thr Gly Thr
275 280 285
Ser Leu Asn Val Leu Pro Ser Ala Leu Ala Glu Leu Leu Asn Lys Glu
290 295 300
Ile Gly Ala Lys Lys Gly Tyr Asn Gly Gln Tyr Thr Ile Glu Cys Asp
305 310 315 320
Lys Arg Ala Ser Leu Pro Asp Ile Thr Phe Asn Leu Ala Gly Ser Asn
325 330 335
Tyr Ser Leu Pro Ala Thr Asp Tyr Ile Leu Glu Val Gln Gly Ser Cys
340 345 350
Ile Ser Thr Phe Gln Gly Met Asp Phe Pro Glu Pro Val Gly Pro Leu
355 360 365
Val Ile Leu Gly Asp Ala Phe Leu Arg Arg Tyr Tyr Ser Val Tyr Asp
370 375 380
Leu Gly Lys Asn Ala Val Gly Leu Ala Arg Ala Lys
385 390 395
<210> 50
<211> 395
<212> PRT
<213> Metarhizium acridum
<400> 50
Met Lys Ser Ala Leu Ile Ala Ala Ala Ala Leu Ala Gly Thr Ala His
1 5 10 15
Ala Gly Val His Lys Met Lys Leu Gln Lys Ile Ser Leu Lys Glu Gln
20 25 30
Leu Ala Asp Ala Pro Ile Glu Gln His Val Gln Ala Leu Gly Gln Lys
35 40 45
Tyr Ile Gly Ala Arg Pro Pro Ser Arg Ala Ser Val Met Phe Asn Thr
50 55 60
Lys Ala Pro Gln Val Ala Gly Gly His Pro Val Pro Val Ser Asn Phe
65 70 75 80
Met Asn Ala Gln Tyr Phe Ser Glu Ile Thr Ile Gly Ser Pro Pro Gln
85 90 95
Ser Phe Lys Val Val Leu Asp Thr Gly Ser Ser Asn Leu Trp Val Pro
100 105 110
Ser Gln Ser Cys Asn Ser Ile Ala Cys Tyr Leu His Ser Thr Tyr Asp
115 120 125
Ser Ser Ser Ser Ser Thr Tyr Lys Lys Asn Gly Ser Ser Phe Glu Ile
130 135 140
Arg Tyr Gly Ser Gly Ser Leu Ser Gly Phe Val Ser Gln Asp Val Val
145 150 155 160
Ser Ile Gly Asp Leu Lys Ile Glu His Gln Asp Phe Ala Glu Ala Thr
165 170 175
Ser Glu Pro Gly Leu Ala Phe Ala Phe Gly Lys Phe Asp Gly Ile Leu
180 185 190
Gly Leu Gly Tyr Asp Thr Leu Ser Val Asn Lys Ile Val Pro Pro Phe
195 200 205
Tyr Gln Met Ile Asp Gln Lys Leu Leu Asp Glu Pro Val Phe Ala Phe
210 215 220
Tyr Leu Gly Ser Lys Glu Glu Gly Ser Glu Ala Val Phe Gly Gly Ile
225 230 235 240
Asp Lys Asn His Tyr Thr Gly Glu Leu Glu Tyr Leu Pro Leu Arg Arg
245 250 255
Lys Ala Tyr Trp Glu Val Asp Ile Asn Ser Ile Ala Leu Gly Asp Glu
260 265 270
Ile Ala Glu Leu Asp His Thr Gly Ala Ile Leu Asp Thr Gly Thr Ser
275 280 285
Leu Asn Val Leu Pro Ser Thr Leu Ala Glu Leu Leu Asn Lys Glu Ile
290 295 300
Gly Ala Lys Lys Ser Trp Asn Gly Gln Tyr Thr Val Asp Cys Asp Lys
305 310 315 320
Ile Lys Ser Leu Pro Asp Ile Val Phe Asn Leu Ser Asn Ser Asn Tyr
325 330 335
Ser Leu Pro Ala Ser Asp Tyr Ile Leu Glu Leu Gln Gly Thr Cys Leu
340 345 350
Ser Thr Phe Gln Gly Met Asp Ile Pro Glu Pro Ala Gly Pro Leu Val
355 360 365
Ile Leu Gly Asp Ala Phe Leu Arg Arg Tyr Tyr Ser Val Tyr Asp Leu
370 375 380
Glu Arg Asn Ala Val Gly Leu Ala Arg Ala Lys
385 390 395
<210> 51
<211> 398
<212> PRT
<213> 大丽轮枝菌
<400> 51
Met Lys Ser Thr Leu Ile Ala Ala Ala Leu Leu Gly Ser Ala Ser Ala
1 5 10 15
Glu Ile His Lys Leu Lys Leu Lys Lys Val Pro Ile Glu Glu Gln Leu
20 25 30
Thr Ser Val Pro Ile Glu Thr Gln Val Gln Gln Leu Gly Gln Lys Tyr
35 40 45
Met Gly Val Arg Pro Gln Ser His Ala Asp Tyr Met Phe Asn Ser Lys
50 55 60
Pro Val Gln Ser Asp Gly Asn His Asn Val Pro Val Ser Asn Phe Met
65 70 75 80
Asn Ala Gln Tyr Phe Ser Glu Ile Thr Ile Gly Thr Pro Pro Gln Thr
85 90 95
Phe Lys Val Val Leu Asp Thr Gly Ser Ser Asn Leu Trp Val Pro Ser
100 105 110
Gln Gln Cys Ser Ser Ile Ala Cys Tyr Leu His Thr Lys Tyr Asp Ser
115 120 125
Ser Asp Ser Ser Thr Tyr Lys Ala Asn Gly Ser Glu Phe Glu Ile His
130 135 140
Tyr Gly Ser Gly Ser Leu Thr Gly Phe Val Ser Gln Asp Thr Val Thr
145 150 155 160
Ile Gly Asp Ile Lys Ile Lys Asn Gln Asp Phe Ala Glu Ala Thr Ser
165 170 175
Glu Pro Gly Leu Ala Phe Ala Phe Gly Arg Phe Asp Gly Ile Leu Gly
180 185 190
Leu Gly Tyr Asp Thr Ile Ser Val Asn Lys Ile Val Pro Pro Phe Tyr
195 200 205
Gln Met Val Asn Gln Lys Ala Val Asp Glu Pro Val Phe Ala Phe Tyr
210 215 220
Leu Gly Asp Thr Asn Glu Gln Gly Asp Glu Ser Glu Val Val Phe Gly
225 230 235 240
Gly Val Asp Glu Ser His Tyr Glu Gly Lys Ile Thr Thr Ile Pro Leu
245 250 255
Arg Arg Lys Ala Tyr Trp Glu Val Asp Leu Asp Ser Ile Ser Leu Gly
260 265 270
Asp Asn Thr Ala Glu Leu Asp Gly His Gly Ala Ile Leu Asp Thr Gly
275 280 285
Thr Ser Leu Asn Val Leu Pro Ser Thr Leu Ala Asp Met Leu Asn Asn
290 295 300
Glu Ile Gly Ala Lys Lys Gly Tyr Asn Gly Gln Trp Ser Val Glu Cys
305 310 315 320
Asp Lys Arg Ala Ser Leu Pro Asp Ile Thr Phe Asn Leu Ala Gly Tyr
325 330 335
Asn Phe Ser Ile Ser Ala Tyr Asp Tyr Ile Leu Glu Val Ser Gly Ser
340 345 350
Cys Ile Ser Thr Phe Gln Gly Met Asp Phe Pro Glu Pro Val Gly Pro
355 360 365
Leu Val Ile Leu Gly Asp Ala Phe Leu Arg Arg Trp Tyr Ser Ile Tyr
370 375 380
Asp Leu Gly Lys Asn Thr Val Gly Leu Ala Lys Ala Lys Lys
385 390 395
<210> 52
<211> 398
<212> PRT
<213> 灰葡萄孢霉
<400> 52
Met Lys Gly Ala Phe Leu Ala Ala Ala Ser Leu Leu Gly Ser Val Ser
1 5 10 15
Ala Gly Val His Lys Met Pro Leu Lys Lys Val Ser Leu Ser Glu Gln
20 25 30
Leu Ala Thr Ala Asn Met Gln Glu His Ala Lys His Leu Gly Gln Lys
35 40 45
Tyr Met Gly Val Arg Pro Glu Ser His Ala Ser Glu Met Phe Lys Glu
50 55 60
Thr Ser Val His Asp Ala Gly Asp His Thr Val Pro Val Ser Asn Phe
65 70 75 80
Leu Asn Ala Gln Tyr Phe Ser Glu Ile Thr Ile Gly Thr Pro Pro Gln
85 90 95
Ser Phe Lys Val Val Leu Asp Thr Gly Ser Ser Asn Leu Trp Val Pro
100 105 110
Ser Ser Gln Cys Gly Ser Ile Ala Cys Tyr Leu His Thr Lys Tyr Asp
115 120 125
Ser Ser Ser Ser Ser Thr Tyr Lys Gln Asn Gly Thr Ser Phe Glu Ile
130 135 140
Arg Tyr Gly Ser Gly Ser Leu Ser Gly Phe Thr Ser Lys Asp Val Met
145 150 155 160
Thr Ile Gly Asp Leu Lys Ile Lys Asp Gln Val Phe Ala Glu Ala Thr
165 170 175
Glu Glu Pro Gly Leu Ala Phe Ala Phe Gly Arg Phe Asp Gly Ile Leu
180 185 190
Gly Leu Gly Tyr Asp Thr Ile Ser Val Asn Ser Ile Val Pro Pro Phe
195 200 205
Tyr Ser Met Val Asp Gln Gly Leu Leu Asp Glu Pro Val Phe Ala Phe
210 215 220
Tyr Leu Gly Ser Asn Asp Glu Ser Asp Pro Ser Glu Ala Ile Phe Gly
225 230 235 240
Gly Val Asn Lys Asp His Tyr Asp Gly Lys Ile Thr Glu Ile Pro Leu
245 250 255
Arg Arg Lys Ala Tyr Trp Glu Val Asp Leu Asp Ser Ile Ala Phe Gly
260 265 270
Asp Ser Glu Ala Glu Leu Glu Asn Thr Gly Val Ile Leu Asp Thr Gly
275 280 285
Thr Ser Leu Ile Ala Leu Pro Ala Asp Leu Ala Gly Leu Leu Asn Ala
290 295 300
Glu Ile Gly Ala Lys Lys Gly Trp Asn Gly Gln Tyr Thr Val Asp Cys
305 310 315 320
Ala Lys Arg Asp Ser Leu Pro Glu Leu Thr Phe Thr Leu Ser Gly His
325 330 335
Lys Phe Pro Ile Gly Pro Tyr Asp Tyr Ile Leu Glu Val Gln Gly Ser
340 345 350
Cys Ile Ser Ala Ile Met Gly Met Asp Phe Pro Glu Pro Val Gly Pro
355 360 365
Leu Ala Ile Leu Gly Asp Ala Phe Leu Arg Arg Tyr Tyr Ser Ile Tyr
370 375 380
Asp Leu Gly Lys Asn Thr Val Gly Leu Ala Lys Ala Lys Ala
385 390 395
<210> 53
<211> 396
<212> PRT
<213> 球毛壳霉
<400> 53
Met Lys Gly Ala Leu Leu Thr Ala Ala Val Leu Leu Gly Ser Ala Gln
1 5 10 15
Gly Ala Val His Lys Met Lys Leu Gln Lys Val Pro Leu Ser Glu Gln
20 25 30
Leu Glu Ala Val Pro Leu Asn Thr Gln Leu Glu Gln Leu Gly Gln Lys
35 40 45
Tyr Met Gly Val Arg Pro Arg Gln Ser His Ala Asn Ala Val Phe Asn
50 55 60
Gly Met Val Ala Glu Val Lys Gly Asn His Pro Val Pro Ile Ser Asn
65 70 75 80
Phe Met Asn Ala Gln Tyr Phe Ser Glu Ile Thr Ile Gly Ser Pro Pro
85 90 95
Gln Thr Phe Lys Val Val Leu Asp Thr Gly Ser Ser Asn Leu Trp Val
100 105 110
Pro Ser Val Glu Cys Gly Ser Ile Ala Cys Tyr Leu His Thr Lys Tyr
115 120 125
Asp Ser Ser Ala Ser Ser Thr Tyr Lys Lys Asn Gly Thr Asn Phe Glu
130 135 140
Ile Arg Tyr Gly Ser Gly Ser Leu Ser Gly Phe Val Ser Gln Asp Thr
145 150 155 160
Met Thr Ile Gly Asp Ile Thr Ile Lys Gly Gln Asp Phe Ala Glu Ala
165 170 175
Thr Ser Glu Pro Gly Leu Ala Phe Ala Phe Gly Arg Phe Asp Gly Ile
180 185 190
Leu Gly Leu Gly Tyr Asp Thr Ile Ser Val Asn Gly Ile Val Pro Pro
195 200 205
Phe Tyr Lys Met Leu Glu Gln Lys Leu Ile Asp Glu Pro Val Phe Ala
210 215 220
Phe Tyr Leu Ala Asp Glu Lys Gly Gln Ser Glu Val Val Phe Gly Gly
225 230 235 240
Val Asp Ser Asp Lys Tyr Lys Gly Lys Ile Thr Thr Ile Pro Leu Arg
245 250 255
Arg Lys Ala Tyr Trp Glu Val Asp Phe Asp Ala Ile Ser Tyr Gly Asp
260 265 270
Asp Thr Ala Glu Leu Glu Asn Thr Gly Val Ile Leu Asp Thr Gly Thr
275 280 285
Ser Leu Ile Ala Leu Pro Ser Gln Leu Ala Glu Met Leu Asn Ala Gln
290 295 300
Ile Gly Ala Lys Lys Asn Tyr Ala Gly Gln Tyr Ala Ile Asp Cys Asn
305 310 315 320
Lys Arg Asp Ser Leu Lys Asp Val Thr Phe Asn Leu Ala Gly Tyr Asn
325 330 335
Phe Thr Leu Gly Pro Tyr Asp Tyr Ile Leu Glu Val Ser Gly Ser Cys
340 345 350
Ile Ser Thr Phe Met Gly Met Asp Phe Pro Glu Pro Thr Gly Pro Leu
355 360 365
Ala Ile Leu Gly Asp Ala Phe Leu Arg Arg Tyr Tyr Ser Ile Tyr Asp
370 375 380
Leu Gly Ala Asn Thr Val Gly Leu Ala Glu Ala Lys
385 390 395
<210> 54
<211> 396
<212> PRT
<213> 太瑞斯梭孢壳霉
<400> 54
Met Lys Gly Ala Leu Leu Thr Ala Ala Val Leu Leu Gly Ser Ala Gln
1 5 10 15
Gly Ala Val His Lys Leu Lys Leu Gln Lys Ile Pro Leu Ala Glu Gln
20 25 30
Leu Glu Ser Val Pro Ile Gln Thr Gln Leu Glu His Leu Gly Gln Lys
35 40 45
Tyr Met Gly Leu Arg Pro Lys Gln Ser His Ala Ser Ala Val Phe Asn
50 55 60
Gly Met Pro Val Glu Val Glu Gly Asn His Pro Val Pro Ile Ser Asn
65 70 75 80
Tyr Met Asn Ala Gln Tyr Phe Ser Glu Ile Thr Leu Gly Thr Pro Pro
85 90 95
Gln Ser Phe Lys Val Val Leu Asp Thr Gly Ser Ser Asn Leu Trp Val
100 105 110
Pro Ser Val Glu Cys Gly Ser Ile Ala Cys Tyr Leu His Ser Lys Tyr
115 120 125
Asp Ser Ser Ala Ser Ser Thr Tyr Lys Lys Asn Gly Thr Ser Phe Asp
130 135 140
Ile Arg Tyr Gly Ser Gly Ser Leu Ser Gly Phe Val Ser Gln Asp Thr
145 150 155 160
Leu Ser Ile Gly Asp Ile Thr Val Lys Gly Gln Asp Phe Ala Glu Ala
165 170 175
Thr Ser Glu Pro Gly Leu Ala Phe Ala Phe Gly Arg Phe Asp Gly Ile
180 185 190
Leu Gly Leu Gly Tyr Asp Thr Ile Ser Val Asn Gly Ile Val Pro Pro
195 200 205
Phe Tyr Lys Met Val Glu Gln Lys Leu Val Asp Glu Pro Val Phe Ala
210 215 220
Phe Tyr Leu Ala Asp Thr Asn Gly Glu Ser Glu Val Val Phe Gly Gly
225 230 235 240
Val Asp Lys Asp Arg Tyr Lys Gly Lys Ile Thr Thr Ile Pro Leu Arg
245 250 255
Arg Lys Ala Tyr Trp Glu Val Asp Phe Glu Ser Leu Ser Tyr Gly Asp
260 265 270
Asp Thr Ala Asp Phe Glu Asn Thr Gly Ala Ile Leu Asp Thr Gly Thr
275 280 285
Ser Leu Ile Thr Leu Pro Ser Gln Leu Ala Glu Met Leu Asn Ala Gln
290 295 300
Leu Gly Ala Lys Lys Asn Phe Ala Gly Gln Tyr Val Leu Asp Cys Ser
305 310 315 320
Lys Arg Asp Ser Leu Glu Asp Ile Thr Phe Asn Leu Ala Gly Tyr Asn
325 330 335
Phe Thr Leu Gly Pro Gln Asp Tyr Ile Leu Glu Ile Ser Gly Ser Cys
340 345 350
Met Ser Thr Phe Thr Pro Met Asp Phe Pro Ala Pro Thr Gly Pro Leu
355 360 365
Ala Ile Leu Gly Asp Ala Phe Leu Arg Arg Tyr Tyr Ser Ile Tyr Asp
370 375 380
Leu Gly Ala Asn Thr Val Gly Leu Ala Glu Ala Asn
385 390 395
<210> 55
<211> 395
<212> PRT
<213> 稻瘟病菌
<400> 55
Met Lys Gly Ala Met Met Thr Ala Ala Val Leu Leu Gly Thr Ala Glu
1 5 10 15
Ala Gly Val His Lys Leu Lys Met Lys Lys Ile Pro Leu Glu Asp Gln
20 25 30
Leu Lys Thr Phe Asp Leu Asn Ala Gln Met Arg Gly Leu Gly Gln Lys
35 40 45
Tyr Leu Gly Ile Arg Pro Glu Ser His Gln Gln Ala Val Phe Ser Asn
50 55 60
Asp Ala Val Gln Ala Ser Gly Asn His Pro Val Pro Ile Ser Asn Phe
65 70 75 80
Met Asn Ala Gln Tyr Phe Ser Glu Ile Thr Ile Gly Thr Pro Pro Gln
85 90 95
Asn Phe Lys Val Ile Leu Asp Thr Gly Ser Ser Asn Leu Trp Val Pro
100 105 110
Ser Ser Ser Cys Gly Ser Ile Ala Cys Tyr Leu His Asn Lys Tyr Glu
115 120 125
Ser Ser Ser Ser Ser Thr Tyr Lys Lys Asn Gly Thr Glu Phe Lys Ile
130 135 140
Gln Tyr Gly Ser Gly Ser Met Glu Gly Phe Val Ser Asn Asp Val Met
145 150 155 160
Thr Ile Gly Asp Leu Lys Ile Lys Asn Leu Asp Phe Ala Glu Ala Thr
165 170 175
Lys Glu Pro Gly Leu Ala Phe Ala Phe Gly Arg Phe Asp Gly Ile Leu
180 185 190
Gly Met Gly Phe Asp Arg Leu Ser Val Asn Lys Ile Val Pro Pro Phe
195 200 205
Tyr Ala Met Val Asp Gln Lys Leu Ile Asp Glu Pro Val Phe Ala Phe
210 215 220
Tyr Leu Ala Asp Glu Lys Ser Glu Ser Glu Val Val Phe Gly Gly Val
225 230 235 240
Asn Lys Asp His Ile Asp Gly Lys Ile Thr Glu Ile Pro Leu Arg Arg
245 250 255
Lys Ala Tyr Trp Glu Val Asp Leu Asp Ala Ile Ala Leu Gly Asp Glu
260 265 270
Val Ala Glu Leu Asp Asn Thr Gly Val Ile Leu Asp Thr Gly Thr Ser
275 280 285
Leu Ile Ala Leu Pro Ser Gln Leu Ala Glu Leu Leu Asn Ser Gln Ile
290 295 300
Gly Ala Lys Lys Gly Tyr Asn Gly Gln Tyr Ser Ile Asp Cys Asp Lys
305 310 315 320
Arg Lys Asp Leu Pro Asp Ile Thr Phe Arg Leu Ser Gly Tyr Asp Phe
325 330 335
Pro Ile Ser Ala Tyr Asp Tyr Ile Leu Glu Val Ser Gly Ser Cys Ile
340 345 350
Ser Thr Phe Met Ala Met Asp Ile Pro Glu Pro Val Gly Pro Leu Ala
355 360 365
Ile Leu Gly Asp Ala Phe Leu Arg Arg Tyr Tyr Ser Ile Tyr Asp Leu
370 375 380
Gly Lys Gly Thr Val Gly Leu Ala Lys Ala Lys
385 390 395
<210> 56
<211> 396
<212> PRT
<213> 黑孢块菌
<400> 56
Met Lys Ser Ser Ile Ile Phe Ala Ala Gly Ser Leu Leu Gly Ser Ala
1 5 10 15
Met Ala Gly Val His Arg Ala Pro Leu Lys Lys Val Pro Leu Thr Glu
20 25 30
Gln Leu Ser His His Asp Ile Asn Thr Gln Met Arg Ala Leu Gly Gln
35 40 45
Lys Tyr Met Gly Ile Arg Pro Glu Lys Ile Asp Glu Glu Met Phe Lys
50 55 60
Thr Gln Glu Ile Lys Thr Asp Asp Gly His Pro Val Pro Val Ser Asn
65 70 75 80
Phe Leu Asn Ala Gln Tyr Phe Ser Glu Ile Thr Ile Gly Thr Pro Pro
85 90 95
Gln Thr Phe Lys Val Val Leu Asp Thr Gly Ser Ser Asn Leu Trp Val
100 105 110
Pro Ser Ser Gln Cys Gly Ser Ile Ala Cys Tyr Leu His Ser Lys Tyr
115 120 125
Asp Ser Ser Thr Ser Ser Thr Tyr Arg Pro Asn Gly Thr Ser Phe Glu
130 135 140
Ile Arg Tyr Gly Ser Gly Ser Leu Ser Gly Phe Val Ser Gln Asp Asn
145 150 155 160
Ile Glu Ile Gly Asn Leu Lys Ile Lys Asp Gln Thr Phe Ala Glu Ala
165 170 175
Thr Ser Glu Pro Gly Leu Ala Phe Ala Phe Gly Arg Phe Asp Gly Ile
180 185 190
Leu Gly Leu Gly Tyr Asp Ser Ile Ser Val Asn His Ile Val Pro Pro
195 200 205
Phe Tyr Gln Met Val Asp Gln Gly Leu Leu Asp Glu Pro Val Phe Ala
210 215 220
Phe Tyr Leu Gly Asp Lys Asp Asp Gln Ser Glu Ala Ile Phe Gly Gly
225 230 235 240
Ile Asp Lys Ala His Tyr Gln Gly Lys Leu Ile Lys Leu Pro Val Arg
245 250 255
Arg Lys Ala Tyr Trp Glu Val Glu Phe Glu Ala Ile Thr Phe Gly Lys
260 265 270
Ser Thr Ala Gln Phe Glu Asn Thr Gly Ala Ile Val Asp Thr Gly Thr
275 280 285
Ser Leu Ile Ala Leu Pro Ser Thr Leu Ala Glu Leu Leu Asn Lys Glu
290 295 300
Ile Gly Ala Lys Lys Gly Phe Asn Gly Gln Tyr Ser Val Glu Cys Glu
305 310 315 320
Lys Arg Asp Ser Leu Pro Asp Leu Thr Phe Thr Leu Thr Gly His Asp
325 330 335
Phe Thr Ile Thr Ala Tyr Asp Tyr Ile Leu Glu Val Gln Gly Ser Cys
340 345 350
Ile Ser Ala Phe Met Gly Met Asp Phe Pro Glu Pro Ile Gly Pro Leu
355 360 365
Ala Ile Leu Gly Asp Ala Phe Leu Arg Arg Tyr Tyr Ser Val Tyr Asn
370 375 380
Leu Gly Asp Asn Thr Ile Gly Leu Ala Lys Ser Lys
385 390 395
<210> 57
<211> 396
<212> PRT
<213> 粗糙链孢霉
<400> 57
Met Lys Gly Ala Leu Leu Thr Ala Ala Met Leu Leu Gly Ser Ala Gln
1 5 10 15
Ala Gly Val His Thr Met Lys Leu Lys Lys Val Pro Leu Ala Glu Gln
20 25 30
Leu Glu Ser Val Pro Ile Asp Val Gln Val Gln His Leu Gly Gln Lys
35 40 45
Tyr Thr Gly Leu Arg Thr Glu Ser His Thr Gln Ala Met Phe Lys Ala
50 55 60
Thr Asp Ala Gln Val Ser Gly Asn His Pro Val Pro Ile Thr Asn Phe
65 70 75 80
Met Asn Ala Gln Tyr Phe Ser Glu Ile Thr Ile Gly Thr Pro Pro Gln
85 90 95
Thr Phe Lys Val Val Leu Asp Thr Gly Ser Ser Asn Leu Trp Val Pro
100 105 110
Ser Ser Gln Cys Gly Ser Ile Ala Cys Tyr Leu His Asn Lys Tyr Glu
115 120 125
Ser Ser Glu Ser Ser Thr Tyr Lys Lys Asn Gly Thr Ser Phe Lys Ile
130 135 140
Glu Tyr Gly Ser Gly Ser Leu Ser Gly Phe Val Ser Gln Asp Arg Met
145 150 155 160
Thr Ile Gly Asp Ile Thr Ile Asn Asp Gln Leu Phe Ala Glu Ala Thr
165 170 175
Ser Glu Pro Gly Leu Ala Phe Ala Phe Gly Arg Phe Asp Gly Ile Leu
180 185 190
Gly Leu Gly Tyr Asp Arg Ile Ala Val Asn Gly Ile Thr Pro Pro Phe
195 200 205
Tyr Lys Met Val Glu Gln Lys Leu Val Asp Glu Pro Val Phe Ser Phe
210 215 220
Tyr Leu Ala Asp Gln Asp Gly Glu Ser Glu Val Val Phe Gly Gly Val
225 230 235 240
Asn Lys Asp Arg Tyr Thr Gly Lys Ile Thr Thr Ile Pro Leu Arg Arg
245 250 255
Lys Ala Tyr Trp Glu Val Asp Phe Asp Ala Ile Gly Tyr Gly Lys Asp
260 265 270
Phe Ala Glu Leu Glu Gly His Gly Val Ile Leu Asp Thr Gly Thr Ser
275 280 285
Leu Ile Ala Leu Pro Ser Gln Leu Ala Glu Met Leu Asn Ala Gln Ile
290 295 300
Gly Ala Lys Lys Ser Trp Asn Gly Gln Phe Thr Ile Asp Cys Gly Lys
305 310 315 320
Lys Ser Ser Leu Glu Asp Val Thr Phe Thr Leu Ala Gly Tyr Asn Phe
325 330 335
Thr Leu Gly Pro Glu Asp Tyr Ile Leu Glu Ala Ser Gly Ser Cys Leu
340 345 350
Ser Thr Phe Met Gly Met Asp Met Pro Ala Pro Val Gly Pro Leu Ala
355 360 365
Ile Leu Gly Asp Ala Phe Leu Arg Lys Tyr Tyr Ser Ile Tyr Asp Leu
370 375 380
Gly Ala Asp Thr Val Gly Ile Ala Thr Ala Lys Arg
385 390 395
<210> 58
<211> 426
<212> PRT
<213> 里氏木霉
<400> 58
Met Lys Phe His Ala Ala Ala Leu Thr Leu Ala Cys Leu Ala Ser Ser
1 5 10 15
Ala Ser Ala Gly Val Ala Gln Pro Arg Ala Asp Glu Val Glu Ser Ala
20 25 30
Glu Gln Gly Lys Thr Phe Ser Leu Glu Gln Ile Pro Asn Glu Arg Tyr
35 40 45
Lys Gly Asn Ile Pro Ala Ala Tyr Ile Ser Ala Leu Ala Lys Tyr Ser
50 55 60
Pro Thr Ile Pro Asp Lys Ile Lys His Ala Ile Glu Ile Asn Pro Asp
65 70 75 80
Leu His Arg Lys Phe Ser Lys Leu Ile Asn Ala Gly Asn Met Thr Gly
85 90 95
Thr Ala Val Ala Ser Pro Pro Pro Gly Ala Asp Ala Glu Tyr Val Leu
100 105 110
Pro Val Lys Ile Gly Thr Pro Pro Gln Thr Leu Pro Leu Asn Leu Asp
115 120 125
Thr Gly Ser Ser Asp Leu Trp Val Ile Ser Thr Asp Thr Tyr Pro Pro
130 135 140
Gln Val Gln Gly Gln Thr Arg Tyr Asn Val Ser Ala Ser Thr Thr Ala
145 150 155 160
Gln Arg Leu Ile Gly Glu Ser Trp Val Ile Arg Tyr Gly Asp Gly Ser
165 170 175
Ser Ala Asn Gly Ile Val Tyr Lys Asp Arg Val Gln Ile Gly Asn Thr
180 185 190
Phe Phe Asn Gln Gln Ala Val Glu Ser Ala Val Asn Ile Ser Asn Glu
195 200 205
Ile Ser Asp Asp Ser Phe Ser Ser Gly Leu Leu Gly Ala Ala Ser Ser
210 215 220
Ala Ala Asn Thr Val Arg Pro Asp Arg Gln Thr Thr Tyr Leu Glu Asn
225 230 235 240
Ile Lys Ser Gln Leu Ala Arg Pro Val Phe Thr Ala Asn Leu Lys Lys
245 250 255
Gly Lys Pro Gly Asn Tyr Asn Phe Gly Tyr Ile Asn Gly Ser Glu Tyr
260 265 270
Ile Gly Pro Ile Gln Tyr Ala Ala Ile Asn Pro Ser Ser Pro Leu Trp
275 280 285
Glu Val Ser Val Ser Gly Tyr Arg Val Gly Ser Asn Asp Thr Lys Tyr
290 295 300
Val Pro Arg Val Trp Asn Ala Ile Ala Asp Thr Gly Thr Thr Leu Leu
305 310 315 320
Leu Val Pro Asn Asp Ile Val Ser Ala Tyr Tyr Ala Gln Val Lys Gly
325 330 335
Ser Thr Phe Ser Asn Asp Val Gly Met Met Leu Val Pro Cys Ala Ala
340 345 350
Thr Leu Pro Asp Phe Ala Phe Gly Leu Gly Asn Tyr Arg Gly Val Ile
355 360 365
Pro Gly Ser Tyr Ile Asn Tyr Gly Arg Met Asn Lys Thr Tyr Cys Tyr
370 375 380
Gly Gly Ile Gln Ser Ser Glu Asp Ala Pro Phe Ala Val Leu Gly Asp
385 390 395 400
Ile Ala Leu Lys Ala Gln Phe Val Val Phe Asp Met Gly Asn Lys Val
405 410 415
Val Gly Phe Ala Asn Lys Asn Thr Asn Val
420 425
<210> 59
<211> 417
<212> PRT
<213> 绿木霉
<400> 59
Met Lys Phe His Ala Ala Ala Leu Thr Leu Ala Tyr Leu Ala Asn Ser
1 5 10 15
Ala Ser Ala Ser Val Ala Gln Pro Arg Ser Glu Ala Ala Gln Ala Pro
20 25 30
Glu Asp Gly Lys Thr Phe Ser Ile Thr Gln Ile Gln Asn Glu Arg Phe
35 40 45
Arg Gly Pro Asn Val Pro Ala Ser Tyr Ile Ala Ala Leu Ala Lys Tyr
50 55 60
Ser Pro Gln Val Ser Glu His Ile Lys His Ala Ile Arg Leu Val Glu
65 70 75 80
Glu Thr Gly Asn Gln Thr Gly Thr Ala Val Ala Thr Pro Ala Pro Gly
85 90 95
Ala Asp Ser Glu Tyr Val Leu Pro Val Lys Ile Gly Thr Pro Pro Gln
100 105 110
Thr Val Pro Leu Asn Leu Asp Thr Gly Ser Ser Asp Leu Trp Val Leu
115 120 125
Ser Thr Asp Thr Tyr Pro Ser Gln Ile Gln Gly Gln Ala Ile Tyr Asp
130 135 140
Pro Asn Ala Ser Asn Thr Ser Arg Arg Gln Thr Gly Leu Ser Trp Leu
145 150 155 160
Ile Arg Tyr Gly Asp Gly Ser Ser Ala Asn Gly Ile Val Tyr Lys Asp
165 170 175
Arg Val Gln Ile Gly Asn Thr Phe Phe Asp Gln Gln Ala Ile Glu Ser
180 185 190
Ala Ile Arg Val Ser Asp Asp Ile Ser Asp Asp Thr Phe Ala Ser Gly
195 200 205
Leu Leu Gly Ala Ala Gly Ser Ala Gly Asn Thr Val Arg Pro Asp Lys
210 215 220
Gln Lys Thr Tyr Leu Asp Asn Ile Lys Asp Gln Leu Ala Lys Pro Leu
225 230 235 240
Phe Thr Ala Asn Leu Lys Lys Gly Lys Pro Gly Asn Tyr Asn Phe Gly
245 250 255
Tyr Ile Asn Ala Ser Glu Tyr Thr Gly Asp Ile Gln Tyr Ala Ala Ile
260 265 270
Asn Pro Lys Gly Pro Leu Trp Glu Ile Ser Val Ser Gly Tyr Arg Val
275 280 285
Gly Ser Asp Glu Ser Lys Tyr Val Ser Arg Val Trp Asn Ala Ile Ala
290 295 300
Asp Thr Gly Thr Thr Leu Leu Leu Thr Pro Thr Asp Ile Val Asn Ala
305 310 315 320
Tyr Tyr Ala Gln Val Asn Gly Ser Thr Met Asn Asp Asp Val Gly Met
325 330 335
Met Val Val Pro Cys Thr Ala Lys Leu Pro Asp Phe Val Phe Gly Leu
340 345 350
Gly Lys Tyr Arg Gly Val Ile Pro Gly Thr Tyr Met Asn Tyr Gly Arg
355 360 365
Met Asn Arg Thr Tyr Cys Tyr Gly Gly Leu Gln Asp Ser Glu Gly Ala
370 375 380
Pro Phe Ala Val Leu Gly Asp Ile Ala Leu Lys Ala Gln Phe Val Val
385 390 395 400
Phe Asp Phe Gly Asn Asn Val Val Gly Phe Ala Asn Lys Asn Thr Thr
405 410 415
Val
<210> 60
<211> 543
<212> PRT
<213> 深绿木霉
<400> 60
Met Glu Tyr Ala Leu Asn Ala Ser Thr Ala Ile His Ala Glu Tyr His
1 5 10 15
Ser Ser Val Ala Val Val Arg Gly Leu His Ala Arg Val Cys Leu Ser
20 25 30
Asn Val Ser Val Val Pro Ser Met Ile Thr Met Lys Glu Met Lys Met
35 40 45
Cys Leu Arg Lys Phe Val Val Ser Pro Val Glu Leu Gln His Phe Pro
50 55 60
Gln Ala Ile Glu Leu Glu Gln Ser Phe Pro His Ala His Ala Leu Gly
65 70 75 80
Ser Ile Pro Gly Gln Val Phe Gly Arg Ile Ile Thr Gly Val Gly Gln
85 90 95
Asp Ile Asp Met Ser Glu Gly Gly Leu Gln His Ser Phe His Gln Ile
100 105 110
Ala Thr Ala Leu Asn Leu Ile Met Lys Phe His Ala Thr Ser Leu Ala
115 120 125
Ile Ala Cys Leu Ala Ser Ser Ala Ser Ala Ala Val Ala Gln Pro Lys
130 135 140
Gly Thr Ala Glu Thr Thr Pro Gln Gly Glu Glu Phe Ser Ile Thr Gln
145 150 155 160
Ile Arg Asn Glu Arg Trp Lys Gly Pro Asn Ala Pro Ala Ala Tyr Ile
165 170 175
Ala Ala Leu Ala Lys Tyr Ser Pro Thr Ile Pro Asp Ser Ile Lys His
180 185 190
Ala Ile Arg Val Asn Pro Gly Leu Arg Gln Lys Phe Gly Lys Leu Ile
195 200 205
Asn Ala Gly Asn Gln Thr Gly Thr Gly Val Ala Thr Pro Ser Pro Gly
210 215 220
Gly Asp Ala Glu Tyr Val Leu Pro Val Gln Ile Gly Thr Pro Pro Gln
225 230 235 240
Thr Leu Pro Leu Asn Leu Asp Thr Gly Ser Ser Asp Leu Trp Val Leu
245 250 255
Ser Thr Asp Thr Tyr Pro Ser Gln Val Glu Gly Gln Pro Leu Tyr Lys
260 265 270
Pro Asn Asn Ser Thr Thr Ser Lys Arg Leu Pro Gly Glu Ser Trp Val
275 280 285
Ile Gln Tyr Gly Asp Gly Ser Ser Ala Asn Gly Ile Val Tyr Thr Asp
290 295 300
Arg Val Gln Ile Gly Asn Thr Phe Phe Asn Thr Gln Ala Ile Glu Ser
305 310 315 320
Ala Ile Asn Val Ser Asp Glu Ile Ser Asp Asp Thr Phe Ser Ser Gly
325 330 335
Leu Leu Gly Ala Ala Phe Ser Thr Ala Asn Thr Val Arg Pro Asp Gln
340 345 350
Gln Lys Thr Tyr Leu Asp Asn Ile Lys Asp Gln Leu Val Lys Pro Val
355 360 365
Phe Thr Ala Asn Leu Lys Lys Gly Lys Pro Gly Asn Tyr Asn Phe Gly
370 375 380
Tyr Ile Asn Ala Ser Glu Tyr Thr Gly Asn Ile Gln Tyr Ala Ala Ile
385 390 395 400
Lys Pro Arg Ser Pro Leu Trp Glu Ile Ser Val Ser Gly Tyr Arg Val
405 410 415
Gly Ser Asn Asp Thr Asn Tyr Val Ala Arg Val Trp Asn Ala Ile Ala
420 425 430
Asp Thr Gly Thr Ser Leu Leu Leu Ala Pro Val Asp Ile Val Asn Ala
435 440 445
Tyr Tyr Lys Gln Ile Ala Gly Ser Lys Leu Asp Ser Gly Tyr Gly Met
450 455 460
Met Val Phe Pro Cys Asn Thr Thr Leu Pro Asp Phe Val Phe Gly Leu
465 470 475 480
Gly Thr Tyr Arg Gly Val Ile Pro Gly Ser Tyr Ile Asn Tyr Gly Lys
485 490 495
Ile Asn Arg Thr Tyr Cys Phe Gly Gly Ile Gln Ser Ser Glu Asp Ala
500 505 510
Pro Phe Ala Val Leu Gly Asp Ile Leu Leu Lys Ala Gln Phe Ala Val
515 520 525
Phe Asp Met Gly Asn Lys Val Val Gly Phe Ala Asn Lys Pro Val
530 535 540
<210> 61
<211> 446
<212> PRT
<213> Metarhizium acridum
<400> 61
Met Lys Ala Thr Ile Ala Ala Ile Val Leu Ala Leu Leu Ala Glu Gly
1 5 10 15
Ala Val Val Gly Glu Arg Val Asp Ala Ala Pro Gln Ala Pro Ala Pro
20 25 30
Arg Thr Asn Gly Arg Ser Phe Thr Leu His Gln Val Val Asn Asn Asn
35 40 45
Phe Gln Gly His Asp Gly Pro Thr Ser Phe Leu Arg Ala His Phe Lys
50 55 60
Tyr Ala Gln Lys Leu Pro Asp Tyr Leu Ser Lys Leu Val Glu Ile Asn
65 70 75 80
Pro Asn Phe Arg Ile Lys Phe Gly Ala Ala Ser Gln Gly Lys Cys Tyr
85 90 95
Pro Glu Val Thr Leu Trp Gln Val Leu Ser Leu Leu Leu Thr Ser Ile
100 105 110
Ile Ala Gly Glu Gln Val Gly Thr Val Glu Ala Asn Pro Ser Pro Leu
115 120 125
Val Asp Thr Glu Tyr Ala Val Glu Ile Gly Ile Gly Thr Pro Pro Gln
130 135 140
Arg Ile Arg Leu Asn Leu Asp Thr Gly Ser Ser Asp Phe Trp Val Phe
145 150 155 160
Ser Thr Asp Thr Thr Pro Ser Met Val His Asp Gln Gly Leu Tyr Asn
165 170 175
Ala Asn Lys Ser Thr Thr Ser His Phe Leu Thr Gly Glu Ser Trp Lys
180 185 190
Ile Lys Tyr Gly Asp Asn Ala Ser Ala Ser Gly Tyr Val Tyr Thr Asp
195 200 205
Arg Val Gln Ile Gly Glu Thr Tyr Val Asp Thr Gln Ala Val Gln Val
210 215 220
Ala Val Asn Val Thr Glu Asp Leu Ser Glu Asp Lys Phe Val Ser Gly
225 230 235 240
Ile Leu Gly Met Ala Asn Ser Ala Ala Asn Thr Val Arg Pro Thr Pro
245 250 255
Gln Arg Thr Tyr Ile Asp Asn Ile Lys Asn Asp Leu Ala Leu Pro Leu
260 265 270
Phe Thr Ala Asn Leu Gln Arg Gln Ala Pro Gly Ser Tyr Asn Phe Gly
275 280 285
Tyr Ile Asp Asp Phe Glu Tyr Thr Gly Asp Ile Ser Tyr Thr Pro Ile
290 295 300
Glu Arg Thr Phe Pro Leu Trp Met Val His Thr Thr Gly Tyr Arg Ile
305 310 315 320
Gly Lys Thr Ser His Asp Lys Ser Ile Asn Ala Ile Val Asp Thr Gly
325 330 335
Thr Ser Leu Leu Leu Leu Pro Glu Thr Val Val Ser Asn Tyr Tyr Ala
340 345 350
Lys Val Lys Gly Ser Ser Val Asp Pro Arg Leu Gly Met Arg Val Val
355 360 365
Pro Cys Asn Ala Asn Leu Pro Asp Phe Tyr Leu Thr Ile Gly Ser Tyr
370 375 380
Arg Gly Arg Val Pro Gly Ser Tyr Met Asn Tyr Gly Arg Val Ser Gly
385 390 395 400
Asn Ser Cys Phe Gly Gly Ile Gln Ser Ser Asp Ala Leu Pro Phe Ser
405 410 415
Val Leu Gly Asp Val Phe Leu Lys Thr Gln Phe Val Val Phe Asp Tyr
420 425 430
Gly Asn Ala Arg Val Gly Phe Ala Arg Lys Asn Leu Tyr Glu
435 440 445
<210> 62
<211> 560
<212> PRT
<213> 尖孢镰刀菌
<400> 62
Met Lys Phe Ser Thr Val Ser Phe Leu Leu Leu Ala Gly Cys Ser Thr
1 5 10 15
Gly Ile Pro His Glu Ile Arg Gly Asp Val Tyr Glu Val Lys Asn Pro
20 25 30
Phe Ser Pro Asn Ala Val Asp Pro Glu Val Glu Gly Val Lys Gly Leu
35 40 45
Gln Lys Arg Thr Ile Asp Pro Lys Gly Asp Gln Ala Leu Val Ala Arg
50 55 60
Gly Asp Val Tyr Glu Val Lys Asn Pro Phe Ser Pro Asn Ala Val Asp
65 70 75 80
Pro Glu Val Glu Gly Val Lys Gly Leu Gln Asp Arg Asp Ile Lys Pro
85 90 95
Arg Asp Val Ser Asn Asn Asp Pro Leu Glu Ala Arg Gly Asp Ile Tyr
100 105 110
Glu Val Lys Asn Pro Phe Ser Pro Asn Ala Val Asp Pro Glu Val Glu
115 120 125
Gly Val Lys Gly Leu Gln Glu Arg Asp Ile Asp Pro Glu Glu Gln Glu
130 135 140
Glu Glu Glu Leu Gly Lys Thr Leu Arg Gly Arg Ala Ile Asp His Glu
145 150 155 160
Asn Asp Pro Asp Ser Ala His Leu Lys Pro Arg Ala Gly Gly Glu Lys
165 170 175
Val Lys Met Asp Gln Leu Thr Asn Thr Arg Tyr Lys Lys Pro His Ala
180 185 190
Glu Ile Ala Leu Ile Gln Ala Tyr Asn Lys Tyr His Lys Pro Leu Pro
195 200 205
Pro Lys Leu Lys Lys Ile Ala Gln His Glu Ala Asp Leu Ile Lys Asn
210 215 220
Lys Leu Gly Met Lys Gly Thr Ala Ser Ala Thr Pro Pro Gln Tyr Tyr
225 230 235 240
Asp Ser Gln Tyr Val Val Pro Val Lys Ile Gly Thr Pro Ala Gln Gln
245 250 255
Thr Tyr Leu Asn Phe Asp Thr Gly Ser Ser Asp Leu Trp Val Phe Ser
260 265 270
Thr Asp Thr Tyr Gln Pro Asp Gln Ala Gly His Ile Leu Tyr Lys Pro
275 280 285
Asp Lys Ser Thr Thr Ser Lys Arg Leu Asn Gly Gln Thr Trp Ser Ile
290 295 300
Arg Tyr Gly Asp Gly Thr Gly Ala Ser Gly Ile Val Tyr Thr Asp Lys
305 310 315 320
Val Gln Val Gly Lys Thr Tyr Val Asn Lys Gln Ala Val Glu Ser Ala
325 330 335
Thr Glu Val Ser Asp Gly Ile Ala Ser Asp Lys Phe Ser His Gly Ile
340 345 350
Met Gly Leu Ala Met Ser Ser Leu Asn Thr Val Arg Pro Thr Pro Gln
355 360 365
Asn Thr Tyr Phe Gln Asn Val Gln Asn Asn Leu Ala Val Pro Val Phe
370 375 380
Thr Ala Asn Leu Gln Lys Gly Lys Ala Gly Asn Tyr Asn Phe Gly Tyr
385 390 395 400
Ile Asp Gln Gly Glu Tyr Tyr Gly Ser Ile Gln Phe Ala Lys Val Lys
405 410 415
Lys Gly Ser Pro Trp Trp Gln Ile Asn Ile Glu Gly Tyr Arg Ile Ala
420 425 430
Ala Gly Ala Pro Trp His Lys Tyr Asn Tyr Ser Ala Ile Val Asp Thr
435 440 445
Gly Thr Thr Leu Leu Leu Leu Pro Gly Val Leu Thr Asn Phe Tyr Tyr
450 455 460
Arg Lys Val Lys Gly Ala Tyr Ile Asp Gln Asp Tyr Gly Val Trp Val
465 470 475 480
Phe Pro Cys Thr Ser Lys Leu Pro Ser Phe Tyr Phe Gly Phe Gly Ser
485 490 495
Tyr Gln Gly Lys Val Pro Gly Asn Tyr Ile Asn Tyr Gly Arg Leu Thr
500 505 510
Asn Thr Val Cys Tyr Gly Gly Ile Gln Ser Ser Asp Gly Ile Gly Phe
515 520 525
Ala Ile Leu Gly Asp Ile Leu Leu Lys Ala Gln Phe Val Val Phe Asp
530 535 540
Leu Lys Gly Gln Arg Val Gly Phe Ala Asn Lys Leu Thr Ile Lys Ser
545 550 555 560
<210> 63
<211> 432
<212> PRT
<213> 蛹虫草
<400> 63
Met Lys Thr Thr Thr Leu Thr Ala Ala Leu Ala Leu Ala Thr Thr Gly
1 5 10 15
Tyr Ser Ala Ala Gln Trp Ser Pro Val Ser Ser Ala Ala Thr Gly Ala
20 25 30
Asn Ala Arg Phe Ser Leu Gln Gln Met Tyr Asn Asp Lys Phe Lys Gly
35 40 45
Val Asn Pro Thr Asp Ala Leu Leu Arg Ala Tyr Ser Arg Tyr Ala Thr
50 55 60
Phe Val Pro Asp His Ile Gln Ala Leu Val Asp Lys Glu Pro Ser Leu
65 70 75 80
Lys Ala Thr Phe His Ala Leu Phe Ser Gly Thr Glu Thr His Ala Leu
85 90 95
Lys Thr Val His Ala Glu Ala Ala Pro Pro Ser Asp Val Asp Ser Glu
100 105 110
Tyr Val Leu Pro Val Ser Leu Gly Thr Pro Pro Gln Ile Leu Pro Leu
115 120 125
Asn Leu Asp Thr Gly Ser Ser Asp Phe Trp Val Phe Ser Thr Ala Thr
130 135 140
Pro Gln Asp Glu Leu Arg Gly Gln Lys Ile Tyr Tyr Pro Gln Asn Ser
145 150 155 160
Thr Thr Ser Lys Thr Leu Pro Gly Glu His Trp Lys Ile Arg Tyr Gly
165 170 175
Asp Asn Ser Thr Ala Asn Gly Asp Val Tyr Ile Asp Arg Ala Ala Ile
180 185 190
Gly Pro Val Gly Phe Asp Lys Gln Ala Ile Gln Val Ala Thr Ala Ile
195 200 205
Ser Ser Ser Ile Ala Glu Asp Gly Phe Phe Ser Gly Ile Leu Gly Met
210 215 220
Ala Ser Ser Tyr Ala Asn Thr Val Lys Pro Thr Lys Gln Val Thr Phe
225 230 235 240
Leu Asp Asn Ile Lys Asp Thr Leu Asp Ala Pro Val Phe Thr Ala Asn
245 250 255
Leu Gln Lys Gly Met Ser Gly Asn Tyr Asn Phe Gly Tyr Ile Asp Ala
260 265 270
Ser Glu His Thr Gly Asp Ile Ala Tyr Thr Pro Ile Asn Thr Asn Ser
275 280 285
Ser Phe Trp Glu Val Glu Leu Ser Gly Tyr Gln Phe Gly Gln Asp Ala
290 295 300
Phe Lys Asn Glu Arg Val Val Gly Ile Val Asp Thr Gly Thr Thr Leu
305 310 315 320
Leu Leu Phe Pro Gln His Val Val Asp Glu Tyr Tyr Gly Lys Leu Pro
325 330 335
Gly Ser Phe Phe Asp Lys Gln Ser Ala Thr Met Val Phe Pro Cys Asp
340 345 350
Leu Thr Pro Pro Asp Phe Phe Phe Gly Val Gly Asn Asp Tyr Arg Gly
355 360 365
Arg Ile Pro Gly His Tyr Met Asn Tyr Ala His Gln Ser Ala Lys Tyr
370 375 380
Cys Tyr Gly Gly Leu Gln Ser Ala Lys Gly Ile Pro Phe Cys Ile Phe
385 390 395 400
Gly Asp Ile Leu Leu Lys Ala Gln Phe Val Val Phe Asp Arg Gly Asn
405 410 415
Leu Ser Val Gly Phe Ala Asn Lys Lys Thr Val Pro Ala Pro Gln Ser
420 425 430
<210> 64
<211> 480
<212> PRT
<213> 玉米赤霉
<400> 64
Met Arg Phe Ser Thr Val Ser Phe Leu Leu Leu Ala Gly Cys Ser Thr
1 5 10 15
Gly Ile Pro His Glu Ile Arg Gly Gly His Glu Val Asn Gly Ala Ser
20 25 30
Val Asp His Pro Glu Lys Pro Leu Gly Arg Ala Glu Arg Arg Ser Thr
35 40 45
Asp Leu Gln Val Asp Gly Ser Arg Ala Leu Thr Val Arg Ser Asp Ala
50 55 60
Tyr Glu Asp Pro Ile Asp Gly Thr Glu Glu Glu Asp Ile Asp Leu Glu
65 70 75 80
Glu Leu Glu Glu Ile Glu Met Gly Lys Gly Leu Lys Gly Arg Ala Ile
85 90 95
Asp Pro Glu Thr Asp Pro Asp Ser Ala His Leu Lys Pro His Ala Glu
100 105 110
Ile Ala Leu Ile His Ala Tyr Asn Lys Tyr Arg Lys Pro Leu Pro Asn
115 120 125
Gln Glu Leu Lys Lys Ile Ala Ala Gln Glu Glu Ala Phe Val Lys Asn
130 135 140
Lys Leu Gly Met Lys Gly Thr Ala Ala Ala Thr Pro Pro Gln Tyr Tyr
145 150 155 160
Asp Ser Gln Tyr Val Val Pro Val Gln Ile Gly Ser Pro Gln Gln Thr
165 170 175
Thr Tyr Leu Asn Phe Asp Thr Gly Ser Ser Asp Leu Trp Val Phe Ser
180 185 190
Thr Asp Thr Tyr Gln Pro Asp Gln Ala Gly His Ile Leu Tyr Lys Pro
195 200 205
Asp Lys Ser Thr Lys Cys Lys Arg Leu Asn Gly Gln Thr Trp Ser Ile
210 215 220
Lys Tyr Gly Asp Gly Thr Gly Ala Ser Gly Ile Val Tyr Leu Asp Thr
225 230 235 240
Val Arg Val Gly Lys Thr Tyr Val Asn Gln Met Ala Val Gln Ser Ala
245 250 255
Thr Glu Val Ser Asp Gly Ile Ala Ser Asp Thr Phe Ala His Gly Ile
260 265 270
Leu Gly Leu Ala Met Ser Ser Leu Asn Thr Val Arg Pro Thr Ala Gln
275 280 285
Lys Thr Tyr Phe Glu Arg Val Gln Gly Ala Leu Glu Val Pro Ile Phe
290 295 300
Thr Ala Asn Leu Lys Lys Gly Ser Ala Gly Asn Tyr Asn Phe Gly Tyr
305 310 315 320
Leu Asp Gln Ser Glu Tyr Tyr Gly Ser Val Gly Phe Ala Ala Val Lys
325 330 335
Lys Asp Ser Pro Trp Trp Gln Ile Thr Val Glu Gly Phe Arg Val Ala
340 345 350
Gln Gly Ala Pro Trp His Lys Tyr Lys Tyr Ser Ala Ile Val Asp Thr
355 360 365
Gly Thr Thr Leu Leu Leu Leu Pro Asp Tyr Leu Val Lys Phe Tyr Tyr
370 375 380
Lys Lys Val Lys Gly Ala Tyr Ile Asp Asn Asp Tyr Gly Val Trp Val
385 390 395 400
Phe Pro Cys Thr Ala Lys Leu Pro Ser Phe Tyr Phe Gly Phe Gly Asn
405 410 415
Tyr Arg Gly Lys Val Pro Gly His Tyr Ile Asn Tyr Gly Arg Leu Thr
420 425 430
Ser Thr Val Cys Tyr Gly Gly Ile Gln Thr Ser Glu Gly Ile Gly Phe
435 440 445
Ala Ile Leu Gly Asp Ile Leu Leu Lys Ala Gln Phe Val Ile Phe Asp
450 455 460
Leu Lys Gly Asn Arg Val Gly Phe Ala Asn Lys Asn Thr Val Lys Ala
465 470 475 480
<210> 65
<211> 432
<212> PRT
<213> 大丽轮枝菌
<400> 65
Met His Tyr Leu Thr Tyr Leu Asn Cys Leu Ala Ala Val Ala Ala Ala
1 5 10 15
Ser Pro Leu Ala Val Ala Glu Pro Ala Asn Ile Asp Gly Ser Thr Phe
20 25 30
Thr Leu Arg Gln Val Glu Asn Lys Asp Phe Ala Gly Gln Thr Gly Leu
35 40 45
Asp Glu Leu Val Trp Ala Tyr Ile Lys Tyr Asn Ala Glu Leu Pro Ala
50 55 60
Lys Val Arg Glu Ala Ile Val Ile Gly Lys His Val Asn Ala Arg Phe
65 70 75 80
Lys Gly Leu Val Gln Arg Gly Thr Thr Asp Asp Lys Ser Ala Ser Phe
85 90 95
Gly Thr Val Gln Ala Phe Pro Pro Arg Asn Val Asp Ile Gln Tyr Val
100 105 110
Val Pro Val Gln Ile Gly Ser Pro Pro Gln Thr Val Phe Leu Asn Leu
115 120 125
Asp Thr Gly Ser Gly Asp Leu Trp Thr Phe Ser Ser Glu Thr Pro Glu
130 135 140
Trp Gln Arg Gly Asp His Arg Ile Tyr Ser Pro Glu Leu Ser Asn Thr
145 150 155 160
Ser Val Leu Gln Asp Gly Leu Thr Trp Arg Ile Thr Tyr Gly Asp Gly
165 170 175
Ser Gly Ala Ser Gly Asn Val Tyr Asn Asp Arg Val Ala Leu Gly Lys
180 185 190
Thr Ser Phe Ala Ala Gln Ala Ile Glu Ser Ala Ser Gln Val Ser Pro
195 200 205
Ser Phe Thr Arg Asp Ser Phe Ala Ser Gly Ile Leu Gly Leu Gly Phe
210 215 220
Gly Arg Gly Asn Thr Val Lys Pro Arg Pro Val Lys Thr Tyr Phe Glu
225 230 235 240
Asn Val Met Pro Ala Leu Ala Ser Pro Val Phe Thr Ser Asn Leu Gln
245 250 255
Ala Gly Arg Pro Gly Asn Tyr Asn Phe Gly Phe Val Ala Arg Asn Glu
260 265 270
Tyr Thr Gly Arg Ile Gly Tyr Thr Pro Val Thr Lys Arg Asn Pro Tyr
275 280 285
Trp Glu Ile Lys Val Asp Gly Thr Gln Ile Gly Gly Gly Glu Tyr Asn
290 295 300
Arg Thr Thr Thr Leu Asp Arg Val Ile Val Asp Thr Gly Thr Thr Leu
305 310 315 320
Leu Met Leu Pro Asp Glu His Val Asp Ala Tyr Tyr Asp Gln Val Glu
325 330 335
Gly Ala Arg Phe Ser Pro Ser Trp Ala Ala Tyr Leu Phe Pro Cys Asp
340 345 350
Ala Thr Leu Pro Asp Trp Thr Phe Gly Leu Gly Asp Tyr Arg Gly Lys
355 360 365
Leu Pro Gly Arg Tyr Val Arg Tyr Asn Gln Val Asn Glu Thr His Cys
370 375 380
Phe Gly Gly Ile Gln Ser Ser Ala Gly Ile Pro Phe Ala Ile Phe Gly
385 390 395 400
Ala Ala Leu Leu Lys Ala Gln Phe Ala Val Phe Asp Tyr Val Glu Ala
405 410 415
Asp Arg Glu Val Gln Pro Arg Val Gly Phe Ala Asn Lys Pro Leu Arg
420 425 430
<210> 66
<211> 259
<212> PRT
<213> 里氏木霉
<400> 66
Met Ala Pro Ala Ser Gln Val Val Ser Ala Leu Met Leu Pro Ala Leu
1 5 10 15
Ala Leu Gly Ala Ala Ile Gln Pro Arg Gly Ala Asp Ile Val Gly Gly
20 25 30
Thr Ala Ala Ser Leu Gly Glu Phe Pro Tyr Ile Val Ser Leu Gln Asn
35 40 45
Pro Asn Gln Gly Gly His Phe Cys Gly Gly Val Leu Val Asn Ala Asn
50 55 60
Thr Val Val Thr Ala Ala His Cys Ser Val Val Tyr Pro Ala Ser Gln
65 70 75 80
Ile Arg Val Arg Ala Gly Thr Leu Thr Trp Asn Ser Gly Gly Thr Leu
85 90 95
Val Gly Val Ser Gln Ile Ile Val Asn Pro Ser Tyr Asn Asp Arg Thr
100 105 110
Thr Asp Phe Asp Val Ala Val Trp His Leu Ser Ser Pro Ile Arg Glu
115 120 125
Ser Ser Thr Ile Gly Tyr Ala Thr Leu Pro Ala Gln Gly Ser Asp Pro
130 135 140
Val Ala Gly Ser Thr Val Thr Thr Ala Gly Trp Gly Thr Thr Ser Glu
145 150 155 160
Asn Ser Asn Ser Ile Pro Ser Arg Leu Asn Lys Val Ser Val Pro Val
165 170 175
Val Ala Arg Ser Thr Cys Gln Ala Asp Tyr Arg Ser Gln Gly Leu Ser
180 185 190
Val Thr Asn Asn Met Phe Cys Ala Gly Leu Thr Gln Gly Gly Lys Asp
195 200 205
Ser Cys Ser Gly Asp Ser Gly Gly Pro Ile Val Asp Ala Asn Gly Val
210 215 220
Leu Gln Gly Val Val Ser Trp Gly Ile Gly Cys Ala Glu Ala Gly Phe
225 230 235 240
Pro Gly Val Tyr Thr Arg Ile Gly Asn Phe Val Asn Tyr Ile Asn Gln
245 250 255
Asn Leu Ala
<210> 67
<211> 255
<212> PRT
<213> 深绿木霉
<400> 67
Met Ala Pro Ala Ser Ala Val Val Ser Ala Leu Met Leu Pro Ala Leu
1 5 10 15
Ala Leu Gly Ala Ala Ile Glu Pro Arg Gly Ala Glu Ile Val Gly Gly
20 25 30
Thr Thr Ala Ala Ala Gly Glu Phe Pro Tyr Ile Val Ser Leu Ser Ser
35 40 45
Gly Gly Ser His Phe Cys Gly Gly Val Leu Ala Asn Ala Asn Thr Val
50 55 60
Ile Thr Ala Gly His Cys Ser Val Asp Phe Ser Ala Ser Gln Val Lys
65 70 75 80
Val Arg Ala Gly Ser Leu Thr Trp Ala Ser Gly Gly Thr Gln Val Gly
85 90 95
Val Ser Ser Ile Thr Val Asn Pro Ser Tyr Thr Thr Lys Asn Gly Val
100 105 110
Pro Asp Phe Asp Val Ala Val Trp Lys Leu Ser Thr Pro Ile Gln Thr
115 120 125
Ser Ser Thr Ile Gly Tyr Val Thr Leu Pro Ala Ser Gly Ser Asp Pro
130 135 140
Ala Ala Gly Thr Thr Leu Thr Thr Ala Gly Trp Gly Thr Thr Ser Glu
145 150 155 160
Asn Ser Asn Ser Leu Pro Ser Arg Leu Asn Lys Val Ser Val Pro Val
165 170 175
Ile Ser Arg Ala Ser Cys Asn Ser Glu Tyr Gly Asn Ile Ile Ser Asn
180 185 190
Asn Met Phe Cys Ala Gly Leu Ser Gln Gly Gly Lys Asp Ser Cys Ser
195 200 205
Gly Asp Ser Gly Gly Pro Ile Val Asp Ser Asn Gly Val Leu Gln Gly
210 215 220
Leu Val Ser Trp Gly Gln Gly Cys Ala Glu Ala Gly Phe Ala Gly Val
225 230 235 240
Tyr Val Arg Leu Gly Asn Leu Leu Ser Phe Val Asn Ser His Leu
245 250 255
<210> 68
<211> 254
<212> PRT
<213> 绿木霉
<400> 68
Met Ala Pro Val Ser Gln Val Val Ser Ala Leu Met Leu Pro Ala Leu
1 5 10 15
Ala Leu Gly Ala Ala Ile Glu Pro Arg Gly Ala Asp Ile Val Gly Gly
20 25 30
Thr Ala Ala Ala Leu Gly Glu Phe Pro Tyr Ile Val Ser Leu Ser Thr
35 40 45
Gln Gly Ser His Phe Cys Gly Gly Val Leu Val Asn Ala Asn Thr Val
50 55 60
Val Thr Ala Ala His Cys Ser Val Asp Phe Thr Ala Ser Gln Val Lys
65 70 75 80
Val Arg Ala Gly Thr Leu Thr Trp Ala Ser Gly Gly Thr Gln Val Gly
85 90 95
Val Ser Lys Ile Ile Val Asn Pro Ser Tyr Asn Ser Arg Thr Thr Asp
100 105 110
Phe Asp Val Ala Val Trp Lys Leu Ala Ser Ser Ile Pro Gln Ser Ser
115 120 125
Thr Ile Gly Tyr Ala Thr Leu Pro Ala Ala Gly Ser Asp Pro Ala Ala
130 135 140
Gly Ser Ile Val Thr Thr Ala Gly Trp Gly Thr Thr Ser Glu Gly Ser
145 150 155 160
Ser Ser Leu Pro Ala Arg Leu Asn Lys Val Ser Ile Pro Val Val Ser
165 170 175
Arg Ala Thr Cys Gln Ser Glu Tyr Gly Arg Ser Ala Ile Thr Thr Asn
180 185 190
Met Phe Cys Ala Ala Gln Ala Gln Gly Gly Lys Asp Ser Cys Gln Gly
195 200 205
Asp Ser Gly Gly Pro Ile Val Asp Ala Asn Gly Val Leu Gln Gly Leu
210 215 220
Val Ser Trp Gly Asn Gly Cys Ala Glu Ala Gly Phe Ala Gly Val Tyr
225 230 235 240
Ser Arg Leu Gly Ala Leu Leu Ser Phe Val Gln Ala Asn Leu
245 250
<210> 69
<211> 254
<212> PRT
<213> 有性哈茨木霉
<400> 69
Met Ala Pro Val Ser Gln Ile Val Ser Ala Leu Met Leu Pro Ala Leu
1 5 10 15
Ala Leu Gly Ala Ala Ile Glu Pro Arg Gly Ala Asp Ile Val Gly Gly
20 25 30
Thr Ala Ala Ala Leu Gly Glu Phe Pro Tyr Ile Val Ser Leu Ser Thr
35 40 45
Gln Gly Ser His Phe Cys Gly Gly Val Leu Val Asn Ala Asn Thr Val
50 55 60
Ile Thr Ala Ala His Cys Ser Val Asp Phe Ser Ala Ser Gln Val Lys
65 70 75 80
Val Arg Ala Gly Thr Leu Thr Trp Ala Ser Gly Gly Thr Gln Val Gly
85 90 95
Val Ser Arg Ile Ile Val Asn Pro Ser Tyr Asn Ser Arg Thr Thr Asp
100 105 110
Phe Asp Val Ala Val Trp Lys Leu Ala Ser Ser Ile Pro Glu Ser Ser
115 120 125
Thr Ile Gly Tyr Ala Thr Leu Pro Ala Ala Gly Ser Asp Pro Ala Ala
130 135 140
Gly Ser Ile Val Thr Thr Ala Gly Trp Gly Thr Thr Ser Glu Gly Ser
145 150 155 160
Ser Ser Leu Pro Ala Arg Leu Asn Lys Val Ser Val Pro Val Val Ser
165 170 175
Arg Ala Thr Cys Gln Ser Glu Tyr Gly Arg Ser Ala Val Thr Thr Asn
180 185 190
Met Phe Cys Ala Ala Gln Ala Gln Gly Gly Lys Asp Ser Cys Gln Gly
195 200 205
Asp Ser Gly Gly Pro Ile Val Asp Ala Asn Gly Val Leu Gln Gly Leu
210 215 220
Val Ser Trp Gly Asn Gly Cys Ala Glu Ala Gly Phe Ala Gly Val Tyr
225 230 235 240
Ser Arg Leu Gly Ser Leu Leu Ser Phe Val Gln Ala Asn Leu
245 250
<210> 70
<211> 258
<212> PRT
<213> 有性哈茨木霉
<400> 70
Met Ala Pro Val Leu Ala Ile Ala Ser Val Leu Ala Ala Leu Pro Ala
1 5 10 15
Leu Thr Met Gly Ala Ala Ile Thr Pro Arg Gly Ser Asp Ile Val Gly
20 25 30
Gly Thr Thr Ala Ala Leu Gly Glu Phe Pro Tyr Ile Val Ser Leu Ser
35 40 45
Thr Gly Gly Ser His Phe Cys Gly Gly Val Leu Ile Asp Ser Arg Thr
50 55 60
Val Val Thr Ala Gly His Cys Thr Ile Asp Gln Arg Ala Ser Ser Val
65 70 75 80
Lys Val Arg Ala Gly Thr Leu Thr Trp Ala Ser Gly Gly Thr Gln Val
85 90 95
Gly Val Ser Ser Leu Thr Leu His Pro Ser Tyr Thr Val Asp Ser Gln
100 105 110
Gly Val Pro Asp Asn Asp Val Gly Val Trp His Leu Ala Thr Ala Ile
115 120 125
Pro Thr Ser Ser Thr Ile Gly Tyr Ala Thr Leu Pro Ala Ser Gly Ser
130 135 140
Asp Pro Ala Ala Gly Thr Thr Leu Thr Val Ala Gly Trp Gly Thr Thr
145 150 155 160
Ser Glu Asn Ser Asn Ser Leu Pro Ser Thr Leu Arg Lys Val Ser Val
165 170 175
Pro Val Val Ala Arg Ala Thr Cys Asp Ser Asp Tyr Asp Gly Glu Ile
180 185 190
Ser Asn Asn Met Phe Cys Ala Ala Val Ala Ala Gly Gly Lys Asp Ser
195 200 205
Cys Ser Gly Asp Ser Gly Gly Pro Ile Ile Asp Pro Ser Gly Thr Leu
210 215 220
Val Gly Val Val Ser Trp Gly Gln Gly Cys Ala Glu Arg Gly Phe Pro
225 230 235 240
Gly Val Tyr Thr Arg Leu Gly Asn Tyr Val Ser Phe Ile Asn Ser Asn
245 250 255
Arg Gly
<210> 71
<211> 271
<212> PRT
<213> 核盘菌
<400> 71
Met Ala Pro Ile Leu Asn Phe Ala Leu Ala Leu Ala Met Pro Ile Leu
1 5 10 15
Ser Met Ala Ala Ala Leu Pro Ser Gly Thr Glu Leu Glu Asn Arg Gly
20 25 30
Thr Thr Leu Thr Gly Thr Val Gly Ile Val Gly Gly Thr Thr Ala Ala
35 40 45
Leu Gly Glu Phe Pro Tyr Ile Val Ser Leu Thr Tyr Ala Gly Ser His
50 55 60
Phe Cys Gly Gly Val Leu Leu Asn Ala Tyr Thr Val Leu Thr Ala Ala
65 70 75 80
His Cys Ser Val Ser Tyr Ser Ala Ser Ser Val Lys Val Arg Ala Gly
85 90 95
Thr Leu Thr Trp Ala Ser Gly Gly Thr Gln Val Gly Val Ser Lys Val
100 105 110
Val Val His Pro Ser Tyr Asn Ser Arg Thr Ile Asp Asn Asp Ile Ala
115 120 125
Leu Trp His Leu Ser Thr Ala Ile Pro Ser Ser Ser Thr Ile Gly Tyr
130 135 140
Ala Lys Leu Pro Val Gln Gly Ser Asp Pro Val Val Gly Ser Thr Ala
145 150 155 160
Thr Val Ala Gly Trp Gly Leu Leu Thr Glu Asn Ser Ser Ser Leu Pro
165 170 175
Ala Thr Leu Arg Lys Val Ser Val Pro Val Ile Ser Arg Ser Thr Cys
180 185 190
Gln Ala Glu Tyr Gly Thr Ser Ser Val Thr Thr Asn Met Trp Cys Ala
195 200 205
Gly Val Thr Gly Gly Gly Lys Asp Ser Cys Ser Gly Asp Ser Gly Gly
210 215 220
Pro Ile Ile Asp Ala Ala Thr Gly Val Leu Glu Gly Thr Val Ser Trp
225 230 235 240
Gly Gln Gly Cys Ala Glu Ala Gly Tyr Ala Gly Val Tyr Ser Arg Val
245 250 255
Gly Asn Tyr Val Thr Tyr Ile Gln Ser Ser Leu Trp Thr Ser Leu
260 265 270
<210> 72
<211> 271
<212> PRT
<213> 灰葡萄孢霉
<400> 72
Met Ala Pro Ile Leu Asn Phe Ala Leu Ala Leu Ala Met Pro Ile Leu
1 5 10 15
Ser Leu Ala Ala Ala Leu Pro Thr Gly Ala Glu Leu Glu Asn Arg Ala
20 25 30
Thr Thr Leu Gly Gly Thr Pro Asp Ile Val Gly Gly Thr Thr Ala Ala
35 40 45
Leu Gly Glu Phe Pro Tyr Ile Val Ser Leu Ser Tyr Ser Gly Ser His
50 55 60
Phe Cys Gly Gly Val Leu Leu Asn Ala Tyr Thr Val Leu Thr Ala Ala
65 70 75 80
His Cys Ser Val Ser Tyr Ser Ala Ser Ser Val Lys Val Arg Ala Gly
85 90 95
Thr Leu Thr Trp Ala Ser Gly Gly Thr Gln Val Gly Val Ser Lys Val
100 105 110
Val Val His Pro Ser Tyr Ser Ser Ser Thr Thr Asn Asn Asp Ile Ala
115 120 125
Leu Trp His Leu Ser Thr Ala Leu Pro Ala Ser Ser Thr Ile Gly Tyr
130 135 140
Ala Lys Leu Pro Val Gln Gly Ser Asp Pro Val Val Gly Ser Thr Thr
145 150 155 160
Thr Val Ala Gly Trp Gly Leu Leu Thr Glu Asn Gly Ser Thr Leu Pro
165 170 175
Ser Thr Leu Arg Lys Val Ser Val Pro Val Ile Ser Arg Ala Ser Cys
180 185 190
Gln Ala Glu Tyr Gly Thr Ser Ala Val Thr Thr Asn Met Trp Cys Ala
195 200 205
Gly Tyr Ala Ala Gly Gly Lys Asp Ser Cys Ser Gly Asp Ser Gly Gly
210 215 220
Pro Ile Ile Asp Thr Ala Thr Gly Val Leu Glu Gly Thr Val Ser Trp
225 230 235 240
Gly Gln Gly Cys Ala Glu Ala Gly Tyr Ala Gly Val Tyr Ala Arg Val
245 250 255
Gly Asn Tyr Val Thr Tyr Ile Asn Ser Asn Leu Trp Thr Ser Leu
260 265 270
<210> 73
<211> 263
<212> PRT
<213> 颖枯壳针孢
<400> 73
Met His Ala Gln Ser Ile Leu Ala Ala Leu Ala Val Pro Ala Leu Val
1 5 10 15
Tyr Gly Ala Ala Ile Pro Gln Glu Phe Pro Glu Asn Trp Asp Glu Thr
20 25 30
Ser Glu Asp Ile Val Gly Gly Val Thr Ala Ala Gln Gly Asp Val Pro
35 40 45
Phe Ile Val Ser Val Ser Leu Ser Gly Val Gly His Tyr Cys Gly Gly
50 55 60
Ser Leu Ile Asn Ala Asn Thr Val Val Ser Ala Ala His Cys Tyr Gln
65 70 75 80
Gly Glu Thr Ala Thr Asp Phe Ser Val Arg Ala Gly Ser Leu Asn Lys
85 90 95
Asn Ser Gly Gly Thr Thr Ser Ala Val Ala Ser Ile Val Ile His Pro
100 105 110
Ser Tyr Asn Ser Arg Thr Ser Asp Asn Asp Ile Ala Ile Ile Lys Leu
115 120 125
Arg Thr Pro Ile Ala Thr Ser Ser Thr Ile Ser Tyr Ala Ser Leu Ala
130 135 140
Ala Ser Gly Gln Asp Pro Ala Ala Gly Thr Thr Leu Thr Val Ala Gly
145 150 155 160
Trp Gly Ala Thr Thr Gln Gly Gly Gly Ser Pro Thr Thr Leu Arg Lys
165 170 175
Val Asp Val Pro Val Val Ala Arg Thr Thr Cys Arg Ser Arg Tyr Ser
180 185 190
Thr Ile Gly Leu Ser Val Thr Asp Gln Met Phe Cys Ala Gly Phe Thr
195 200 205
Ala Gly Gly Lys Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro Ile Ile
210 215 220
Thr Ser Ser Lys Gln Leu Val Gly Ile Val Ser Trp Gly Glu Gly Cys
225 230 235 240
Ala Gln Pro Asn Phe Pro Gly Val Tyr Ser Arg Val Gly Ser Leu Thr
245 250 255
Ser Phe Ile Ser Ala Asn Leu
260
<210> 74
<211> 265
<212> PRT
<213> Leptosphaeria maculans
<400> 74
Met Gln Ile Lys Asp Leu Val Val Ala Leu Ala Val Pro Ala Ile Cys
1 5 10 15
Ile Ala Ala Ala Ile Pro Thr Pro Gln Asp Pro Val Glu Thr Glu Phe
20 25 30
Pro Glu Glu Ser Gln Glu Asp Ile Val Gly Gly Thr Ala Ala Ala Ala
35 40 45
Gly Glu Phe Pro Tyr Ile Val Ser Leu Gln Arg Ser Gly Ser His Phe
50 55 60
Cys Gly Gly Ser Leu Leu Asn Gly Asn Thr Val Val Thr Ala Ala His
65 70 75 80
Cys Ala Val Gly Leu Ser Gly Thr Ala Val Thr Val Arg Ala Gly Ser
85 90 95
Asn Ser Arg Thr Ser Gly Gly Val Thr Ala Arg Ser Ser Ser Ile Val
100 105 110
Ile Asn Pro Gly Tyr Ser Ser Ser Thr Phe Asp Gly Asp Val Ala Ile
115 120 125
Ile Lys Leu Ser Thr Ser Ile Pro Thr Ser Asn Thr Ile Ser Tyr Val
130 135 140
Ser Leu Pro Ala Ala Gly Ser Asp Pro Ala Ala Gly Ser Val Ala Thr
145 150 155 160
Val Ala Gly Trp Gly Thr Leu Thr Ser Gly Ser Gln Ser Leu Pro Val
165 170 175
Asn Leu Gln Lys Val Asp Val Pro Ile Val Ser Arg Thr Thr Cys Arg
180 185 190
Ser Asn Tyr Gly Gln Ser Ala Ile Thr Asn Asn Met Ile Cys Ala Gly
195 200 205
Leu Thr Gln Gly Gly Lys Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro
210 215 220
Ile Val Ser Ser Ser Lys Gln Leu Ile Gly Leu Val Ser Trp Gly Asp
225 230 235 240
Gly Cys Ala Ala Pro Gly Lys Pro Gly Val Tyr Thr Arg Val Ala Ala
245 250 255
Val Leu Ser Phe Ile Asn Ala Asn Ser
260 265
<210> 75
<211> 256
<212> PRT
<213> 大丽轮枝菌
<400> 75
Met Val Phe Lys Thr Leu Val Ala Ala Leu Gly Phe Thr Ala Val Ala
1 5 10 15
Phe Gly Ser Pro Val Pro Ala Pro Gln Glu Glu Leu Gln Ile Val Gly
20 25 30
Gly Val Ala Ala Ser Ala Gly Asp Phe Pro Phe Ile Val Ser Leu Gln
35 40 45
Arg Ser Gly Ser His Phe Cys Gly Gly Ser Leu Leu Asn Ala Asn Thr
50 55 60
Val Leu Thr Ala Ala His Cys Ser Thr Gly Val Ser Ala Ser Ser Val
65 70 75 80
Thr Val Arg Ala Gly Ser Leu Asn Arg Ser Ser Gly Gly Thr Leu Val
85 90 95
Arg Val Ser Ser Ile Val Val Asn Pro Gly Tyr Val Ala Ser Arg Tyr
100 105 110
Asp Asn Asp Phe Ala Ile Trp Lys Leu Ala Thr Pro Ile Pro Thr Ser
115 120 125
Ser Thr Ile Ser Tyr Ala Thr Leu Ala Ala Ala Asn Ser Asp Pro Ala
130 135 140
Ala Asn Thr Leu Thr Thr Thr Ala Gly Trp Gly Thr Thr Ser Ser Gly
145 150 155 160
Gly Ser Thr Leu Pro Thr Ala Leu Arg Lys Val Asp Val Pro Ile Ile
165 170 175
Ser Arg Thr Thr Cys Arg Ser Tyr Tyr Gly Thr Ser Ala Val Thr Thr
180 185 190
Asn Met Ile Cys Ala Gly Phe Ala Ala Gly Gly Lys Asp Ser Cys Gln
195 200 205
Gly Asp Ser Gly Gly Pro Ile Ile Glu Ala Ser Ser Arg Thr Leu Val
210 215 220
Gly Val Val Ser Trp Gly Glu Gly Cys Ala Ala Pro Asn Ala Pro Gly
225 230 235 240
Val Tyr Ala Arg Val Gly Ala Ala Arg Thr Phe Ile Asn Gln Tyr Leu
245 250 255
<210> 76
<211> 261
<212> PRT
<213> 炭色孢腔菌
<400> 76
Met Arg Phe Gln Ser Met Ile Thr Ala Ala Leu Pro Ala Leu Val Leu
1 5 10 15
Ser Ala Pro Thr Pro Gln Trp Asp Asp Val Pro Glu Asp Ser Ile Val
20 25 30
Gly Gly Thr Thr Ala Ala Ala Gly Glu Tyr Pro Phe Ile Val Ser Ile
35 40 45
Gln Leu Gly Gly Arg His Asn Cys Gly Gly Thr Leu Ile Asn Gly Asn
50 55 60
Thr Val Val Thr Ala Ala His Cys Ser Val Ser Ser Ala Ile Gly Gly
65 70 75 80
Ser Ile Asn Asn Val Ala Val Arg Val Gly Ser Leu Ser Ala Asn Ser
85 90 95
Gly Gly Gln Val Ile Lys Val Ser Lys Ile Ile Ile His Pro Ser Tyr
100 105 110
Gln Ala Ser Thr Ser Asn Asn Asp Ile Ala Ile Trp Lys Leu Ser Ser
115 120 125
Thr Val Thr Ala Gly Gly Asn Ile Gly Phe Ala Ser Leu Ala Ala Ser
130 135 140
Gly Ser Asp Pro Ala Ser Gly Ser Thr Thr Ser Val Ala Gly Trp Gly
145 150 155 160
Ala Thr Arg Glu Gly Gly Gly Ala Asn Asn Ala Leu Leu Lys Val Ser
165 170 175
Val Pro Ile Val Ala Arg Ser Thr Cys Val Ser Asn Tyr Asn Ala Val
180 185 190
Gly Leu Thr Val Thr Thr Asn Met Val Cys Ala Gly Val Thr Ala Gly
195 200 205
Gly Arg Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro Leu Val Asp Ala
210 215 220
Asn Lys Thr Leu Ile Gly Val Val Ser Trp Gly Thr Gly Cys Ala Arg
225 230 235 240
Pro Asn Leu Pro Gly Val Tyr Ser Arg Val Gly Thr Leu Arg Ser Phe
245 250 255
Ile Asp Gln Asn Ala
260
<210> 77
<211> 257
<212> PRT
<213> Metarhizium acridum
<400> 77
Met Val Ser Arg Ser Ala Ile Ala Ile Ala Ser Ala Val Phe Ala Val
1 5 10 15
Gln Ser Ala Thr Ala Ala Pro Ala Lys Gly Gly Lys Phe Ile Val Gly
20 25 30
Gly Gln Ala Ala Asn Glu Gly Glu Phe Pro Phe Ile Val Thr Val Leu
35 40 45
Leu Asn Gly Thr His Tyr Cys Gly Gly Thr Leu Val Asn Glu Asp Thr
50 55 60
Val Val Thr Ala Ser His Cys Thr Lys Ser Asp Val Ser Gly Tyr Glu
65 70 75 80
Ile Arg Ala Gly Ser Leu Ala Trp Ala Ser Gly Gly Thr Lys Val Lys
85 90 95
Val Ala Ser Ala Val Arg His Pro Gly Tyr Asn Ser Thr Asn Tyr Asp
100 105 110
Asn Asp Val Ala Val Trp Lys Leu Ala Thr Pro Ile Pro Glu Ser Ser
115 120 125
Thr Ile Gln Tyr Ala Lys Leu Pro Ala Gln Gly Ser Asp Pro Ala Ser
130 135 140
Gly Ser Asn Ala Thr Val Ala Gly Trp Gly Arg Leu Thr Glu Gly Gly
145 150 155 160
Thr Val Pro Ala Arg Leu Gln Lys Val Thr Val Pro Val Val Asp Arg
165 170 175
Gly Thr Cys Lys Val Ala Tyr Ser Thr Pro Thr Pro Ser Glu Val Thr
180 185 190
Glu Asn Met Phe Cys Ala Gly Leu Glu Gln Gly Gly Gln Asp Ala Cys
195 200 205
Gln Gly Asp Ser Gly Gly Pro Ile Val Asp Thr Ala Thr Gly Ala Leu
210 215 220
Ile Gly Val Val Ser Trp Gly Val Gly Cys Ala Arg Pro Asp Lys Tyr
225 230 235 240
Gly Val Tyr Thr Arg Leu Gly Asn Tyr Val Asp Phe Val Glu Lys Tyr
245 250 255
Leu
<210> 78
<211> 255
<212> PRT
<213> 金龟子绿僵菌
<400> 78
Met Ala Ser Lys Ala Ala Leu Ala Ile Ala Ser Ala Ile Phe Ala Val
1 5 10 15
Gln Ser Ala Thr Ala Ala Pro Ala Lys Asn Gly Lys Phe Ile Val Gly
20 25 30
Gly Glu Ala Ala Ala Gln Gly Glu Phe Pro Tyr Ile Val Ala Leu Leu
35 40 45
Ser Gly Asn Phe Gln Phe Cys Gly Gly Thr Leu Val Asn Glu Asp Thr
50 55 60
Val Val Thr Ala Gly His Cys Thr Ser Ser Asp Val Ser Gly Ile Glu
65 70 75 80
Val Arg Ala Gly Ser Leu Ala Trp Ala Ser Gly Gly Thr Lys Val Lys
85 90 95
Val Arg Ser Ala Thr Arg His Pro Asp Tyr Asn Gly Asn Asn Tyr Asp
100 105 110
Asn Asp Val Ala Val Trp Lys Leu Ala Thr Ala Ile Pro Gln Ser Ala
115 120 125
Thr Ile Lys Tyr Ala Lys Leu Pro Ala Pro Asp Ser Asp Pro Ala Pro
130 135 140
Gly Ala Asn Val Thr Val Ala Gly Trp Gly Arg Leu Gln Glu Gly Gly
145 150 155 160
Ala Thr Pro Ser Gln Leu Gln Lys Val Thr Val Pro Val Val Asp Arg
165 170 175
Ala Thr Cys Lys Glu Ala Tyr Ser Thr Pro Thr Pro Leu Glu Ile Thr
180 185 190
Asp Asn Met Phe Cys Ala Gly Leu Pro Gln Gly Gly Gln Asp Ala Cys
195 200 205
Gln Gly Asp Ser Gly Gly Pro Ile Val Gln Gly Asp Val Leu Leu Gly
210 215 220
Val Val Ser Trp Gly Val Gly Cys Ala Arg Pro Asn Lys Tyr Gly Val
225 230 235 240
Tyr Thr Arg Leu Gly Asn Tyr Val Ser Phe Ile Glu Gln Tyr Leu
245 250 255
<210> 79
<211> 248
<212> PRT
<213> 尖孢镰刀菌
<400> 79
Met Val Lys Phe Ala Ser Val Val Ala Leu Val Ala Pro Leu Ala Ala
1 5 10 15
Ala Ala Pro Gln Glu Ile Pro Asn Ile Val Gly Gly Thr Ser Ala Ser
20 25 30
Ala Gly Asp Phe Pro Phe Ile Val Ser Ile Ser Arg Asn Gly Gly Pro
35 40 45
Trp Cys Gly Gly Ser Leu Leu Asn Ala Asn Thr Val Leu Thr Ala Ala
50 55 60
His Cys Val Ser Gly Tyr Ala Gln Ser Gly Phe Gln Ile Arg Ala Gly
65 70 75 80
Ser Leu Ser Arg Thr Ser Gly Gly Ile Thr Ser Ser Leu Ser Ser Val
85 90 95
Arg Val His Pro Ser Tyr Ser Gly Asn Asn Asn Asp Leu Ala Ile Leu
100 105 110
Lys Leu Ser Thr Ser Ile Pro Ser Gly Gly Asn Ile Gly Tyr Ala Arg
115 120 125
Leu Ala Ala Ser Gly Ser Asp Pro Val Ala Gly Ser Ser Ala Thr Val
130 135 140
Ala Gly Trp Gly Ala Thr Ser Glu Gly Gly Ser Ser Thr Pro Val Asn
145 150 155 160
Leu Leu Lys Val Thr Val Pro Ile Val Ser Arg Ala Thr Cys Arg Ala
165 170 175
Gln Tyr Gly Thr Ser Ala Ile Thr Asn Gln Met Phe Cys Ala Gly Val
180 185 190
Ser Ser Gly Gly Lys Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro Ile
195 200 205
Val Asp Ser Ser Asn Thr Leu Ile Gly Ala Val Ser Trp Gly Asn Gly
210 215 220
Cys Ala Arg Pro Asn Tyr Ser Gly Val Tyr Ala Ser Val Gly Ala Leu
225 230 235 240
Arg Ser Phe Ile Asp Thr Tyr Ala
245
<210> 80
<211> 252
<212> PRT
<213> 玉米赤霉
<400> 80
Met Val Lys Ile Thr Ser Leu Val Ala Leu Ala Ala Pro Leu Val Ala
1 5 10 15
Ala Ala Pro Asn Pro Gln Asn Ser Pro Gln Ile Val Gly Gly Thr Ser
20 25 30
Ala Ser Ala Gly Glu Phe Pro Phe Ile Val Ser Ile Thr Asn Asn Gly
35 40 45
Gly Pro Trp Cys Gly Gly Thr Leu Leu Asn Ala Asn Thr Val Met Thr
50 55 60
Ala Ser His Cys Val Gln Gly Arg Ser Ala Ser Ala Phe Ala Ile Arg
65 70 75 80
Val Gly Ser Asn Ser Arg Thr Ser Gly Gly Val Thr Ser Arg Val Ser
85 90 95
Ser Ile Arg Met His Pro Ser Phe Ser Gly Ser Thr Leu Asn Asn Asp
100 105 110
Val Ala Leu Leu Lys Leu Ser Thr Ser Ile Pro Ala Gly Gly Ser Ile
115 120 125
Ala Tyr Gly Arg Leu Ala Thr Ser Gly Ser Asp Pro Ala Ala Gly Ser
130 135 140
Ser Leu Thr Val Ala Gly Trp Gly Asp Thr Ser Glu Gly Gly Gly Val
145 150 155 160
Ser Pro Val Asn Leu Leu Lys Val Thr Val Pro Val Val Ser Arg Ala
165 170 175
Thr Cys Arg Ser Gln Tyr Gly Thr Ser Ala Ile Thr Asp Asn Met Phe
180 185 190
Cys Ala Gly Val Thr Gly Gly Gly Lys Asp Ala Cys Gln Gly Asp Ser
195 200 205
Gly Gly Pro Ile Val Asp Ser Ser Lys Thr Val Val Gly Ile Val Ser
210 215 220
Trp Gly Asp Gly Cys Ala Arg Pro Asn Ala Ala Gly Val Tyr Ala Arg
225 230 235 240
Val Gly Thr Leu Arg Ser Trp Ile Asp Ser Asn Ala
245 250
<210> 81
<211> 256
<212> PRT
<213> 金龟子绿僵菌
<400> 81
Met Glu Leu Lys Ser Leu Leu Ala Val Tyr Leu Pro Leu Cys Ala Ala
1 5 10 15
Pro Leu Ala Ala Ala Arg Pro Ala Ser Asn Ala Val Phe Ile Val Gly
20 25 30
Gly Ser Pro Ala Ala Ala Gly Glu Phe Pro Phe Ile Val Ser Thr Leu
35 40 45
Leu Asn Gly Arg His Trp Cys Gly Gly Val Leu Leu Asn Ala Asn Thr
50 55 60
Val Leu Thr Ala Ala His Cys Val Glu Ser Thr Pro Ala Ile Ser Gln
65 70 75 80
Val Arg Ala Gly Ser Leu Ala His Ala Ser Gly Gly Val Val Ala Asn
85 90 95
Ile Ser Ser Ile Thr Pro His Pro Lys Tyr Glu Gly Leu Gly Tyr Asp
100 105 110
Met Ala Ile Leu Lys Leu Ser Thr Pro Ile Glu Ala Asn Gly Thr Ile
115 120 125
Gly Tyr Ala Thr Leu Pro Glu Ala Gly Ser Asp Pro Val Ala Gly Ala
130 135 140
Asp Ala Thr Val Ala Gly Trp Gly Asp Leu Glu Tyr Ala Gly Gln Ala
145 150 155 160
Pro Glu Glu Leu Gln Lys Val Thr Val Pro Val Val Asp Arg Ala Thr
165 170 175
Cys Ser Ala Ala Tyr Gln Ala Ile Pro Asn Met Pro Asn Ile Thr Asp
180 185 190
Ala Met Phe Cys Ala Gly Leu Lys Glu Gly Gly Gln Asp Ala Cys Asn
195 200 205
Gly Asp Ser Gly Gly Pro Ile Ile Asp Thr Glu Thr Arg Val Leu Ile
210 215 220
Gly Val Val Ser Trp Gly Tyr Lys Cys Ala Ala Pro Asn Ala Tyr Gly
225 230 235 240
Val Tyr Thr Arg Leu Gly Ala Asp Ile Glu Phe Ile Lys Ser His Leu
245 250 255
<210> 82
<211> 882
<212> PRT
<213> 里氏木霉
<400> 82
Met Val Arg Ser Ala Leu Phe Val Ser Leu Leu Ala Thr Phe Ser Gly
1 5 10 15
Val Ile Ala Arg Val Ser Gly His Gly Ser Lys Ile Val Pro Gly Ala
20 25 30
Tyr Ile Phe Glu Phe Glu Asp Ser Gln Asp Thr Ala Asp Phe Tyr Lys
35 40 45
Lys Leu Asn Gly Glu Gly Ser Thr Arg Leu Lys Phe Asp Tyr Lys Leu
50 55 60
Phe Lys Gly Val Ser Val Gln Leu Lys Asp Leu Asp Asn His Glu Ala
65 70 75 80
Lys Ala Gln Gln Met Ala Gln Leu Pro Ala Val Lys Asn Val Trp Pro
85 90 95
Val Thr Leu Ile Asp Ala Pro Asn Pro Lys Val Glu Trp Val Ala Gly
100 105 110
Ser Thr Ala Pro Thr Leu Glu Ser Arg Ala Ile Lys Lys Pro Pro Ile
115 120 125
Pro Asn Asp Ser Ser Asp Phe Pro Thr His Gln Met Thr Gln Ile Asp
130 135 140
Lys Leu Arg Ala Lys Gly Tyr Thr Gly Lys Gly Val Arg Val Ala Val
145 150 155 160
Ile Asp Thr Gly Ile Asp Tyr Thr His Pro Ala Leu Gly Gly Cys Phe
165 170 175
Gly Arg Gly Cys Leu Val Ser Phe Gly Thr Asp Leu Val Gly Asp Asp
180 185 190
Tyr Thr Gly Phe Asn Thr Pro Val Pro Asp Asp Asp Pro Val Asp Cys
195 200 205
Ala Gly His Gly Ser His Val Ala Gly Ile Ile Ala Ala Gln Glu Asn
210 215 220
Pro Tyr Gly Phe Thr Gly Gly Ala Pro Asp Val Thr Leu Gly Ala Tyr
225 230 235 240
Arg Val Phe Gly Cys Asp Gly Gln Ala Gly Asn Asp Val Leu Ile Ser
245 250 255
Ala Tyr Asn Gln Ala Phe Glu Asp Gly Ala Gln Ile Ile Thr Ala Ser
260 265 270
Ile Gly Gly Pro Ser Gly Trp Ala Glu Glu Pro Trp Ala Val Ala Val
275 280 285
Thr Arg Ile Val Glu Ala Gly Val Pro Cys Thr Val Ser Ala Gly Asn
290 295 300
Glu Gly Asp Ser Gly Leu Phe Phe Ala Ser Thr Ala Ala Asn Gly Lys
305 310 315 320
Lys Val Ile Ala Val Ala Ser Val Asp Asn Glu Asn Ile Pro Ser Val
325 330 335
Leu Ser Val Ala Ser Tyr Lys Ile Asp Ser Gly Ala Ala Gln Asp Phe
340 345 350
Gly Tyr Val Ser Ser Ser Lys Ala Trp Asp Gly Val Ser Lys Pro Leu
355 360 365
Tyr Ala Val Ser Phe Asp Thr Thr Ile Pro Asp Asp Gly Cys Ser Pro
370 375 380
Leu Pro Asp Ser Thr Pro Asp Leu Ser Asp Tyr Ile Val Leu Val Arg
385 390 395 400
Arg Gly Thr Cys Thr Phe Val Gln Lys Ala Gln Asn Val Ala Ala Lys
405 410 415
Gly Ala Lys Tyr Leu Leu Tyr Tyr Asn Asn Ile Pro Gly Ala Leu Ala
420 425 430
Val Asp Val Ser Ala Val Pro Glu Ile Glu Ala Val Gly Met Val Asp
435 440 445
Asp Lys Thr Gly Ala Thr Trp Ile Ala Ala Leu Lys Asp Gly Lys Thr
450 455 460
Val Thr Leu Thr Leu Thr Asp Pro Ile Glu Ser Glu Lys Gln Ile Gln
465 470 475 480
Phe Ser Asp Asn Pro Thr Thr Gly Gly Ala Leu Ser Gly Tyr Thr Thr
485 490 495
Trp Gly Pro Thr Trp Glu Leu Asp Val Lys Pro Gln Ile Ser Ser Pro
500 505 510
Gly Gly Asn Ile Leu Ser Thr Tyr Pro Val Ala Leu Gly Gly Tyr Ala
515 520 525
Thr Leu Ser Gly Thr Ser Met Ala Cys Pro Leu Thr Ala Ala Ala Val
530 535 540
Ala Leu Ile Gly Gln Ala Arg Gly Thr Phe Asp Pro Ala Leu Ile Asp
545 550 555 560
Asn Leu Leu Ala Thr Thr Ala Asn Pro Gln Leu Phe Asn Asp Gly Glu
565 570 575
Lys Phe Tyr Asp Phe Leu Ala Pro Val Pro Gln Gln Gly Gly Gly Leu
580 585 590
Ile Gln Ala Tyr Asp Ala Ala Phe Ala Thr Thr Leu Leu Ser Pro Ser
595 600 605
Ser Leu Ser Phe Asn Asp Thr Asp His Phe Ile Lys Lys Lys Gln Ile
610 615 620
Thr Leu Lys Asn Thr Ser Lys Gln Arg Val Thr Tyr Lys Leu Asn His
625 630 635 640
Val Pro Thr Asn Thr Phe Tyr Thr Leu Ala Pro Gly Asn Gly Tyr Pro
645 650 655
Ala Pro Phe Pro Asn Asp Ala Val Ala Ala His Ala Asn Leu Lys Phe
660 665 670
Asn Leu Gln Gln Val Thr Leu Pro Ala Gly Arg Ser Ile Thr Val Asp
675 680 685
Val Phe Pro Thr Pro Pro Arg Asp Val Asp Ala Lys Arg Leu Ala Leu
690 695 700
Trp Ser Gly Tyr Ile Thr Val Asn Gly Thr Asp Gly Thr Ser Leu Ser
705 710 715 720
Val Pro Tyr Gln Gly Leu Thr Gly Ser Leu His Lys Gln Lys Val Leu
725 730 735
Tyr Pro Glu Asp Ser Trp Ile Ala Asp Ser Thr Asp Glu Ser Leu Ala
740 745 750
Pro Val Glu Asn Gly Thr Val Phe Thr Ile Pro Ala Pro Gly Asn Ala
755 760 765
Gly Pro Asp Asp Lys Leu Pro Ser Leu Val Val Ser Pro Ala Leu Gly
770 775 780
Ser Arg Tyr Val Arg Val Asp Leu Val Leu Leu Ser Ala Pro Pro His
785 790 795 800
Gly Thr Lys Leu Lys Thr Val Lys Phe Leu Asp Thr Thr Ser Ile Gly
805 810 815
Gln Pro Ala Gly Ser Pro Leu Leu Trp Ile Ser Arg Gly Ala Asn Pro
820 825 830
Ile Ala Trp Thr Gly Glu Leu Ser Asp Asn Lys Phe Ala Pro Pro Gly
835 840 845
Thr Tyr Lys Ala Val Phe His Ala Leu Arg Ile Phe Gly Asn Glu Lys
850 855 860
Lys Lys Glu Asp Trp Asp Val Ser Glu Ser Pro Ala Phe Thr Ile Lys
865 870 875 880
Tyr Ala
<210> 83
<211> 884
<212> PRT
<213> 深绿木霉
<400> 83
Met Val Arg Ser Ala Leu Phe Val Ser Leu Leu Ala Thr Ile Thr Gly
1 5 10 15
Ala Ile Ala Arg Gly Ser Gly His Ala Ser Lys Ile Val Pro Gly Ala
20 25 30
Tyr Ile Phe Glu Phe Glu Asn Asp His Asp Thr Ala Glu Phe Tyr Lys
35 40 45
Gln Leu Asn Gly Glu Gly Ser Thr Arg Met Lys Phe Asp Tyr Lys Leu
50 55 60
Phe Lys Gly Val Ser Val Gln Leu Lys Asp Leu Ser Asn His Glu Asp
65 70 75 80
Lys Ala Gln Lys Met Ala Gln Leu Pro Ala Val Lys Asn Met Trp Pro
85 90 95
Val Thr Leu Ile Gln Ala Pro Asn Pro Lys Val Glu Trp Val Ala Gly
100 105 110
Thr Asn Ser Leu Pro Ser Ile Gln Ser Arg Asp Val Tyr Pro Pro Pro
115 120 125
Thr Asp Asp Gly Asn Phe Pro Pro His Lys Met Val Gln Ile Asp Lys
130 135 140
Leu Arg Ala Lys Gly Tyr Thr Gly Lys Gly Val Lys Val Ala Val Ile
145 150 155 160
Asp Thr Gly Ile Asp Tyr Thr His Pro Ala Leu Gly Gly Cys Tyr Gly
165 170 175
Ala Gly Cys Leu Val Ser Phe Gly Thr Asp Leu Val Gly Asp Asn Tyr
180 185 190
Ser Gly Phe Asn Thr Pro Val Pro Asp Asn Asp Pro Lys Asp Cys Ala
195 200 205
Gly His Gly Thr His Val Ala Gly Ile Ile Ala Ala Gln Lys Asn Gln
210 215 220
Tyr Gly Phe Thr Gly Gly Ala Pro Asp Val Thr Leu Gly Ala Tyr Arg
225 230 235 240
Val Phe Gly Cys Glu Gly Gln Ala Gly Asn Asp Val Leu Ile Ala Ala
245 250 255
Tyr Asn Lys Ala Phe Glu Asp Gly Ala Gln Ile Ile Thr Ala Ser Ile
260 265 270
Gly Gly Pro Ser Gly Trp Ala Glu Glu Pro Trp Ala Val Ala Val Thr
275 280 285
Arg Ile Val Asn Ala Gly Val Pro Cys Thr Val Ser Ala Gly Asn Asp
290 295 300
Gly Asp His Gly Leu Phe Tyr Ala Ser Thr Ala Ala Asn Gly Lys Lys
305 310 315 320
Val Leu Ala Val Ala Ser Val Asp Asn Gln Asn Ile Pro Thr Val Phe
325 330 335
Ser Leu Ser Ser Tyr Lys Ile Asp Ser Gly Ala Asp Thr Lys Phe Gly
340 345 350
Tyr Val Pro Ser Ser Lys Gly Ala Glu Gly Trp Lys Gly Val Thr Lys
355 360 365
Pro Val Tyr Ala Leu Thr Leu Asp Thr Thr Ile Ala Asp Asp Gly Cys
370 375 380
Ser Pro Leu Pro Asp Asn Thr Pro Asp Leu Ser Asp His Ile Val Leu
385 390 395 400
Val Arg Arg Gly Ser Cys Thr Phe Val Gln Lys Ala Gln Asn Val Ala
405 410 415
Ala Lys Gly Gly Lys Tyr Leu Leu Tyr Tyr Asn Thr Ile Ala Gly Ala
420 425 430
Leu Ser Val Glu Val Ser Ser Val Pro Gln Ile Ile Gly Ala Gly Met
435 440 445
Val Glu Asp Ser Thr Gly Ala Thr Trp Ile Ala Ala Ile Lys Ala Gly
450 455 460
Lys Thr Val Thr Leu Ser Met Val Ala Leu Asn Glu Ala Thr Lys Gln
465 470 475 480
Val Gln Phe Ala Asp Asn Lys Ala Thr Gly Gly Ala Leu Ser Thr Tyr
485 490 495
Thr Ser Trp Gly Pro Thr Trp Glu Met Asp Val Lys Pro Gln Ile Ser
500 505 510
Ser Pro Gly Gly Asn Ile Leu Ser Thr Tyr Pro Val Ala Leu Gly Gly
515 520 525
Tyr Ala Thr Leu Ser Gly Thr Ser Met Ala Cys Pro Leu Thr Ala Ala
530 535 540
Ala Val Ala Leu Val Ala Gln Ala Arg Ser Ser Phe Asp Pro Val Leu
545 550 555 560
Leu Glu Asn Leu Leu Ala Thr Thr Ala Asn Pro Gln Leu Phe Asn Asp
565 570 575
Gly Thr Gln Phe Tyr Asp Phe Leu Ala Pro Val Ala Gln Gln Gly Gly
580 585 590
Gly Leu Ile Gln Ala Tyr Asp Ala Ala Phe Ala Thr Thr Leu Leu Ser
595 600 605
Pro Ser Ser Leu Ser Phe Asn Asp Thr Asp His Phe Ile Ser Lys Arg
610 615 620
Gln Ile Thr Leu Lys Asn Thr Ser Lys Asn Arg Val Thr Tyr Lys Ile
625 630 635 640
Gly His Val Pro Thr Asp Thr Phe Tyr Thr Leu Ala Asn Ser Ser Gly
645 650 655
Tyr Ala Ala Pro Phe Pro Asn Asp Ala Val Ala Ser His Ala Ser Val
660 665 670
Arg Leu Ser Gln Gln Gln Val Thr Val Pro Ala Gly Arg Ser Ile Thr
675 680 685
Val Asp Val Phe Pro Thr Pro Pro Arg Gly Val Asp Ala Lys Arg Leu
690 695 700
Gly Leu Trp Ser Gly Tyr Ile Thr Val Asn Gly Thr Asp Gly Ser His
705 710 715 720
Leu Ser Val Pro Tyr Gln Gly Leu Thr Gly Ser Leu Gln Lys Gln Gln
725 730 735
Val Leu Phe Pro Glu Asp Ser Trp Ile Ser Asn Ser Thr Asp Thr Asn
740 745 750
Leu Asn Pro Val Ala Asn Gly Thr Val Phe Thr Ile Pro Gly Pro Gly
755 760 765
Asn Ala Gly Pro Ser Asp Val Leu Pro Ala Leu Val Val Ser Pro Ala
770 775 780
Leu Gly Thr Arg Leu Val Arg Ala Asp Leu Val Leu Leu Ser Ala Pro
785 790 795 800
Pro His Gly Ser Lys Ile Thr Thr Lys Lys Phe Phe Asp Thr Thr Ser
805 810 815
Ile Gly Gln Pro Ser Gly Ser Pro Leu Leu Trp Gln Ser Arg Gly Pro
820 825 830
Thr Pro Ile Ser Trp Ser Gly Glu Leu Thr Asp Asn Lys Phe Ala Pro
835 840 845
Pro Gly Thr Tyr Lys Val Val Phe His Ala Leu Arg Ile Phe Gly Asn
850 855 860
Glu Lys Lys Lys Glu Asp Trp Tyr Val Ser Glu Ser Pro Ala Phe Thr
865 870 875 880
Ile Lys Tyr Ala
<210> 84
<211> 827
<212> PRT
<213> 深绿木霉
<400> 84
Met Lys Phe Asp Tyr Lys Leu Phe Lys Gly Val Ser Val Gln Leu Lys
1 5 10 15
Asp Leu Ser Asn His Glu Asp Lys Ala Gln Lys Met Ala Gln Leu Pro
20 25 30
Ala Val Lys Asn Met Trp Pro Val Thr Leu Ile Gln Ala Pro Asn Pro
35 40 45
Lys Val Glu Trp Val Ala Gly Thr Asn Ser Leu Pro Ser Ile Gln Ser
50 55 60
Arg Asp Val Tyr Pro Pro Pro Thr Asp Asp Gly Asn Phe Pro Pro His
65 70 75 80
Lys Met Val Gln Ile Asp Lys Leu Arg Ala Lys Gly Tyr Thr Gly Lys
85 90 95
Gly Val Lys Val Ala Val Ile Asp Thr Gly Ile Asp Tyr Thr His Pro
100 105 110
Ala Leu Gly Gly Cys Tyr Gly Ala Gly Cys Leu Val Ser Phe Gly Thr
115 120 125
Asp Leu Val Gly Asp Asn Tyr Ser Gly Phe Asn Thr Pro Val Pro Asp
130 135 140
Asn Asp Pro Lys Asp Cys Ala Gly His Gly Thr His Val Ala Gly Ile
145 150 155 160
Ile Ala Ala Gln Lys Asn Gln Tyr Gly Phe Thr Gly Gly Ala Pro Asp
165 170 175
Val Thr Leu Gly Ala Tyr Arg Val Phe Gly Cys Glu Gly Gln Ala Gly
180 185 190
Asn Asp Val Leu Ile Ala Ala Tyr Asn Lys Ala Phe Glu Asp Gly Ala
195 200 205
Gln Ile Ile Thr Ala Ser Ile Gly Gly Pro Ser Gly Trp Ala Glu Glu
210 215 220
Pro Trp Ala Val Ala Val Thr Arg Ile Val Asn Ala Gly Val Pro Cys
225 230 235 240
Thr Val Ser Ala Gly Asn Asp Gly Asp His Gly Leu Phe Tyr Ala Ser
245 250 255
Thr Ala Ala Asn Gly Lys Lys Val Leu Ala Val Ala Ser Val Asp Asn
260 265 270
Gln Asn Ile Pro Thr Val Phe Ser Leu Ser Ser Tyr Lys Ile Asp Ser
275 280 285
Gly Ala Asp Thr Lys Phe Gly Tyr Val Pro Ser Ser Lys Gly Ala Glu
290 295 300
Gly Trp Lys Gly Val Thr Lys Pro Val Tyr Ala Leu Thr Leu Asp Thr
305 310 315 320
Thr Ile Ala Asp Asp Gly Cys Ser Pro Leu Pro Asp Asn Thr Pro Asp
325 330 335
Leu Ser Asp His Ile Val Leu Val Arg Arg Gly Ser Cys Thr Phe Val
340 345 350
Gln Lys Ala Gln Asn Val Ala Ala Lys Gly Gly Lys Tyr Leu Leu Tyr
355 360 365
Tyr Asn Thr Ile Ala Gly Ala Leu Ser Val Glu Val Ser Ser Val Pro
370 375 380
Gln Ile Ile Gly Ala Gly Met Val Glu Asp Ser Thr Gly Ala Thr Trp
385 390 395 400
Ile Ala Ala Ile Lys Ala Gly Lys Thr Val Thr Leu Ser Met Val Ala
405 410 415
Leu Asn Glu Ala Thr Lys Gln Val Gln Phe Ala Asp Asn Lys Ala Thr
420 425 430
Gly Gly Ala Leu Ser Thr Tyr Thr Ser Trp Gly Pro Thr Trp Glu Met
435 440 445
Asp Val Lys Pro Gln Ile Ser Ser Pro Gly Gly Asn Ile Leu Ser Thr
450 455 460
Tyr Pro Val Ala Leu Gly Gly Tyr Ala Thr Leu Ser Gly Thr Ser Met
465 470 475 480
Ala Cys Pro Leu Thr Ala Ala Ala Val Ala Leu Val Ala Gln Ala Arg
485 490 495
Ser Ser Phe Asp Pro Val Leu Leu Glu Asn Leu Leu Ala Thr Thr Ala
500 505 510
Asn Pro Gln Leu Phe Asn Asp Gly Thr Gln Phe Tyr Asp Phe Leu Ala
515 520 525
Pro Val Ala Gln Gln Gly Gly Gly Leu Ile Gln Ala Tyr Asp Ala Ala
530 535 540
Phe Ala Thr Thr Leu Leu Ser Pro Ser Ser Leu Ser Phe Asn Asp Thr
545 550 555 560
Asp His Phe Ile Ser Lys Arg Gln Ile Thr Leu Lys Asn Thr Ser Lys
565 570 575
Asn Arg Val Thr Tyr Lys Ile Gly His Val Pro Thr Asp Thr Phe Tyr
580 585 590
Thr Leu Ala Asn Ser Ser Gly Tyr Ala Ala Pro Phe Pro Asn Asp Ala
595 600 605
Val Ala Ser His Ala Ser Val Arg Leu Ser Gln Gln Gln Val Thr Val
610 615 620
Pro Ala Gly Arg Ser Ile Thr Val Asp Val Phe Pro Thr Pro Pro Arg
625 630 635 640
Gly Val Asp Ala Lys Arg Leu Gly Leu Trp Ser Gly Tyr Ile Thr Val
645 650 655
Asn Gly Thr Asp Gly Ser His Leu Ser Val Pro Tyr Gln Gly Leu Thr
660 665 670
Gly Ser Leu Gln Lys Gln Gln Val Leu Phe Pro Glu Asp Ser Trp Ile
675 680 685
Ser Asn Ser Thr Asp Thr Asn Leu Asn Pro Val Ala Asn Gly Thr Val
690 695 700
Phe Thr Ile Pro Gly Pro Gly Asn Ala Gly Pro Ser Asp Val Leu Pro
705 710 715 720
Ala Leu Val Val Ser Pro Ala Leu Gly Thr Arg Leu Val Arg Ala Asp
725 730 735
Leu Val Leu Leu Ser Ala Pro Pro His Gly Ser Lys Ile Thr Thr Lys
740 745 750
Lys Phe Phe Asp Thr Thr Ser Ile Gly Gln Pro Ser Gly Ser Pro Leu
755 760 765
Leu Trp Gln Ser Arg Gly Pro Thr Pro Ile Ser Trp Ser Gly Glu Leu
770 775 780
Thr Asp Asn Lys Phe Ala Pro Pro Gly Thr Tyr Lys Val Val Phe His
785 790 795 800
Ala Leu Arg Ile Phe Gly Asn Glu Lys Lys Lys Glu Asp Trp Tyr Val
805 810 815
Ser Glu Ser Pro Ala Phe Thr Ile Lys Tyr Ala
820 825
<210> 85
<211> 878
<212> PRT
<213> 绿木霉
<400> 85
Met Val Arg Ser Ala Leu Phe Val Ser Leu Leu Ala Thr Ile Ser Gly
1 5 10 15
Val Ile Ala Arg Gly Ser Gly His Pro Ser Lys Val Val Pro Gly Ala
20 25 30
Tyr Ile Phe Glu Phe Glu Asn Asp Gln Asp Thr Ala Asp Phe Tyr Lys
35 40 45
Gln Leu Asn Gly Glu Gly Ser Thr Arg Met Lys Phe Asn Tyr Lys Leu
50 55 60
Phe Lys Gly Val Ser Val Gln Leu Lys Asp Leu Ser Asn His Glu Asp
65 70 75 80
Lys Ala Gln Lys Met Ala Gln Leu Pro Ala Val Lys Asn Met Trp Pro
85 90 95
Val Thr Leu Ile Gln Ala Pro Asn Pro Lys Val Glu Trp Val Ala Ser
100 105 110
Ser Ser Ser Pro Ala Leu Gln Ala Arg Asp Val Ser Pro Thr Asp Pro
115 120 125
Ser Asn Phe Pro Pro Gln Gln Met Thr Gln Ile Asp Lys Leu Arg Ala
130 135 140
Lys Gly Phe Thr Gly Lys Gly Val Lys Leu Ala Val Ile Asp Thr Gly
145 150 155 160
Ile Asp Tyr Thr His Pro Ala Leu Gly Gly Cys Phe Gly Ala Gly Cys
165 170 175
Leu Val Ser Phe Gly Thr Asp Leu Val Gly Asp Asn Tyr Ser Gly Phe
180 185 190
Asn Thr Pro Val Pro Asp Asn Asp Pro Met Asp Cys Ala Gly His Gly
195 200 205
Ser His Val Ala Gly Ile Ile Ala Ala Gln Lys Asn Pro Tyr Gly Phe
210 215 220
Thr Gly Gly Ala Pro Asp Val Thr Leu Gly Ala Tyr Arg Val Phe Gly
225 230 235 240
Cys Ala Gly Gln Ala Gly Asn Asp Val Leu Ile Ala Ala Tyr Asn Lys
245 250 255
Ala Phe Glu Asp Gly Ala Gln Ile Ile Thr Ala Ser Ile Gly Gly Pro
260 265 270
Ser Gly Trp Ala Glu Glu Pro Trp Ala Val Ala Val Ser Arg Ile Val
275 280 285
Asp Ala Gly Val Pro Cys Thr Val Ser Ala Gly Asn Asp Gly Asp His
290 295 300
Gly Leu Phe Tyr Ala Ser Thr Ala Ala Asn Gly Lys Lys Val Leu Ala
305 310 315 320
Val Ala Ser Val Asp Asn Ala Asn Ile Pro Thr Val Phe Ser Leu Ser
325 330 335
Ser Tyr Lys Val Asp Asn Gly Asp Ala Gln Gln Phe Gly Tyr Val Ser
340 345 350
Ser Ala Lys Ala Trp Lys Gly Val Asn Lys Pro Ile Tyr Ala Val Thr
355 360 365
Leu Asp Thr Thr Ile Pro Asn Asp Ala Cys Asp Pro Leu Pro Asp Ser
370 375 380
Thr Pro Asp Leu Ser Asn Tyr Ile Val Leu Val Arg Arg Gly Thr Cys
385 390 395 400
Thr Phe Val Gln Lys Ala Gln Asn Val Ala Ala Lys Gly Ala Lys Tyr
405 410 415
Leu Leu Tyr Tyr Asn Thr Ala Ala Gly Ala Leu Ala Val Asp Val Ser
420 425 430
Thr Val Thr Gln Ile Thr Ala Ala Gly Met Val Gln Asp Ser Thr Gly
435 440 445
Ala Ala Trp Ile Ala Ala Leu Lys Asp Gly Lys Thr Val Thr Leu Ser
450 455 460
Met Val Asn Ser Asp Glu Ala Thr Lys Gln Ile Gln Phe Ala Asp Asn
465 470 475 480
Lys Val Thr Gly Gly Ala Leu Ser Thr Tyr Thr Ser Trp Gly Pro Thr
485 490 495
Trp Glu Val Asp Val Lys Pro Gln Ile Ser Ser Pro Gly Gly Asn Ile
500 505 510
Leu Ser Thr Tyr Pro Val Ala Lys Gly Gly Tyr Ala Thr Leu Ser Gly
515 520 525
Thr Ser Met Ala Cys Pro Leu Thr Ala Ala Ala Val Ala Leu Val Gly
530 535 540
Gln Ala Arg Lys Thr Phe Asp Pro Val Leu Leu Glu Asn Leu Leu Ala
545 550 555 560
Thr Thr Ala Asn Pro Gln Leu Phe Asn Asp Gly Ala Gln Phe Tyr Asn
565 570 575
Phe Leu Ala Pro Val Ala Gln Gln Gly Gly Gly Leu Ile Gln Ala Tyr
580 585 590
Asp Ala Ala Phe Ala Thr Thr Leu Leu Ser Pro Ser Ser Leu Ser Phe
595 600 605
Asn Asp Thr Asp His Phe Val Arg Lys Arg Gln Ile Thr Leu Lys Asn
610 615 620
Thr Ser Lys Gln Arg Val Thr Tyr Lys Leu Asn His Val Pro Thr Asn
625 630 635 640
Thr Phe Tyr Thr Leu Thr Gly Asp Ser Pro Tyr Pro Ala Pro Phe Pro
645 650 655
Asn Asp Ala Ile Ala Ala His Ala Thr Val Arg Leu Gly Leu Glu Gln
660 665 670
Val Thr Leu Pro Ala Gly Arg Ser Ile Thr Ile Asp Val Leu Pro Thr
675 680 685
Pro Pro Arg Gly Val Asp Ala Lys Arg Leu Gly Leu Trp Ser Gly Tyr
690 695 700
Ile Thr Val Asn Gly Thr Asp Gly Ser Ala Leu Ser Val Pro Tyr Gln
705 710 715 720
Gly Leu Thr Gly Ser Leu His Lys Gln Lys Val Leu Tyr Pro Ala Asp
725 730 735
Ser Trp Ile Ser Lys Ser Thr Asp Glu Asn Leu Asn Pro Val Asp Asn
740 745 750
Gly Thr Val Phe Thr Ile Pro Ala Pro Gly Asn Ala Gln Ser Thr Asp
755 760 765
Val Leu Pro Ser Leu Val Val Ser Pro Ala Phe Gly Ser Arg Leu Val
770 775 780
Arg Val Asp Leu Val Leu Leu Ser Ala Pro Pro Lys Asn Thr Lys Val
785 790 795 800
Lys Thr Thr Lys Phe Leu Asp Thr Thr Ser Ile Gly Gln Pro Ala Gly
805 810 815
Ser Pro Leu Leu Trp Ala Ser Arg Gly Ala Thr Pro Leu Ser Trp Ser
820 825 830
Gly Glu Leu Thr Asp Asn Lys Phe Ala Pro Pro Gly Thr Tyr Lys Ala
835 840 845
Val Phe His Ala Leu Arg Ile Phe Gly Asn Glu Lys Lys Lys Glu Asp
850 855 860
Trp Asp Val Ser Glu Ser Pro Ala Phe Thr Ile Lys Tyr Ala
865 870 875
<210> 86
<211> 878
<212> PRT
<213> 有性哈茨木霉
<400> 86
Met Val Arg Ser Ala Val Phe Leu Ser Leu Leu Ala Thr Ile Ser Gly
1 5 10 15
Val Ile Ala Arg Gly Ser Gly Gln Thr Ser Lys Val Val Pro Gly Ala
20 25 30
Tyr Ile Phe Glu Phe Glu Asn Asp Gln Asp Thr Ala Ala Phe Tyr Lys
35 40 45
Gln Leu Asn Gly Glu Gly Ser Thr Arg Met Lys Phe Asn Tyr Lys Leu
50 55 60
Phe Lys Gly Val Ser Val Gln Leu Lys Asp Leu Ser Asn His Glu Ser
65 70 75 80
Lys Ala Gln Lys Met Ala Gln Leu Pro Ala Val Lys Asn Met Trp Pro
85 90 95
Val Thr Leu Ile Gln Ala Pro Asn Pro Lys Val Glu Trp Val Ala Ser
100 105 110
Ser Thr Ser Pro Ala Leu Gln Ala Arg Asp Val Ser Pro Thr Asp Pro
115 120 125
Ser Asn Phe Pro Pro His Gln Met Val Gln Ile Asp Lys Leu Arg Ala
130 135 140
Lys Gly Tyr Thr Gly Lys Gly Val Lys Val Ala Val Ile Asp Thr Gly
145 150 155 160
Ile Asp Tyr Thr His Pro Ala Leu Gly Gly Cys Phe Gly Ala Gly Cys
165 170 175
Leu Val Ser Phe Gly Thr Asp Leu Val Gly Asp Asn Tyr Ser Gly Phe
180 185 190
Asn Thr Pro Val Pro Asp Asn Asp Pro Lys Asp Cys Ala Gly His Gly
195 200 205
Ser His Val Ala Gly Ile Ile Ala Ala Gln Glu Asn Pro Tyr Gly Phe
210 215 220
Thr Gly Gly Ala Pro Asp Val Thr Leu Gly Ala Tyr Arg Val Phe Gly
225 230 235 240
Cys Glu Gly Gln Ala Gly Asn Asp Val Leu Ile Ala Ala Tyr Asn Lys
245 250 255
Ala Phe Glu Asp Gly Ala Gln Ile Ile Thr Ala Ser Ile Gly Gly Pro
260 265 270
Ser Gly Trp Ala Glu Glu Pro Trp Ala Val Ala Val Ser Arg Ile Val
275 280 285
Asp Ala Gly Val Pro Cys Thr Val Ser Ala Gly Asn Asp Gly Asp His
290 295 300
Gly Leu Phe Phe Ala Ser Thr Ala Ala Asn Gly Lys Lys Val Leu Ala
305 310 315 320
Val Ala Ser Val Asp Asn Lys Asn Ile Pro Thr Val Phe Ser Leu Ser
325 330 335
Ser Tyr Lys Val Asp Asn Gly Asp Ser Gln Gln Phe Gly Tyr Val Ser
340 345 350
Ser Ser Lys Ala Trp Lys Gly Val Thr Lys Pro Val Tyr Ala Val Thr
355 360 365
Leu Asp Thr Thr Ile Pro Asp Asp Ala Cys Ser Pro Leu Pro Asp Ser
370 375 380
Thr Pro Asp Leu Ser Asn Tyr Ile Val Leu Val Arg Arg Gly Thr Cys
385 390 395 400
Thr Phe Val Gln Lys Ala Gln Asn Val Ala Ala Lys Gly Ala Lys Tyr
405 410 415
Leu Leu Tyr Tyr Asn Asn Ile Ala Gly Ala Leu Ser Val Asp Val Ser
420 425 430
Thr Val Thr Gln Ile Thr Ala Ala Gly Met Val Gln Asp Ser Thr Gly
435 440 445
Ala Ala Phe Ile Ala Ala Ile Lys Ala Gly Lys Thr Val Thr Leu Ser
450 455 460
Met Val Asp Pro Asp Ser Ala Thr Lys Gln Ile Gln Phe Ala Asp Asn
465 470 475 480
Lys Ala Thr Gly Gly Ala Leu Ser Thr Tyr Thr Ser Trp Gly Pro Thr
485 490 495
Trp Glu Val Asp Val Lys Pro Gln Ile Ser Ser Pro Gly Gly Asn Ile
500 505 510
Leu Ser Thr Tyr Pro Val Ala Leu Gly Gly Tyr Ala Thr Leu Ser Gly
515 520 525
Thr Ser Met Ala Cys Pro Leu Thr Ala Ala Ala Val Ala Leu Val Ala
530 535 540
Gln Ala Arg Gly Thr Phe Asp Pro Ala Leu Ile Glu Asn Leu Leu Ala
545 550 555 560
Thr Thr Ala Asn Pro Gln Val Phe Asn Asp Gly Ala Gln Phe Tyr Asp
565 570 575
Phe Leu Ala Pro Val Ala Gln Gln Gly Gly Gly Leu Ile Gln Ala Tyr
580 585 590
Asp Ala Ala Phe Ala Thr Thr Leu Leu Ser Pro Ser Ser Leu Thr Phe
595 600 605
Asn Asp Thr Asp His Phe Val Arg Lys Arg Gln Ile Thr Leu Lys Asn
610 615 620
Thr Ser Lys Gln Arg Val Thr Tyr Lys Leu Asn His Val Pro Thr Asn
625 630 635 640
Thr Phe Tyr Thr Leu Ser Gly Asp Ser Pro Tyr Pro Ala Ala Phe Pro
645 650 655
Asn Glu Ala Ile Ala Ala His Ala Ser Val Lys Leu Ala Leu Gln Thr
660 665 670
Val Thr Leu Pro Ala Gly Lys Ser Ile Thr Ile Asp Val Leu Pro Thr
675 680 685
Pro Pro Lys Gly Val Asp Ala Lys Arg Leu Gly Leu Trp Ser Gly Tyr
690 695 700
Ile Thr Val Asn Gly Thr Asp Gly Ser Ala Leu Ser Val Pro Tyr Gln
705 710 715 720
Gly Leu Thr Gly Ser Leu His Lys Gln Gln Val Leu Leu Pro Glu Asp
725 730 735
Ser Trp Ile Ala Lys Ser Thr Asp Glu Asn Leu Asn Pro Val Glu Asn
740 745 750
Gly Thr Val Phe Thr Ile Pro Ala Pro Gly Asn Ala Gln Ser Thr Asp
755 760 765
Val Leu Pro Ser Leu Val Val Ser Pro Ala Phe Gly Ser Arg Gln Ile
770 775 780
Arg Val Asp Leu Val Leu Leu Ser Ala Pro Pro Lys Asn Thr Lys Ile
785 790 795 800
Lys Thr Thr Lys Phe Leu Asp Thr Thr Ser Ile Gly Gln Pro Ala Gly
805 810 815
Ala Pro Val Leu Trp Ala Ser Arg Gly Pro Thr Pro Leu Ser Trp Ser
820 825 830
Gly Glu Leu Thr Asp Asn Lys Phe Ala Pro Pro Gly Thr Tyr Lys Ala
835 840 845
Val Phe His Ala Leu Arg Ile Phe Gly Asn Glu Lys Lys Lys Glu Asp
850 855 860
Trp Asp Val Ser Glu Ser Pro Ala Phe Thr Ile Lys Tyr Ala
865 870 875
<210> 87
<211> 885
<212> PRT
<213> Metarhizium acridum
<400> 87
Met Val Gly Leu Ser Leu Val Val Ser Leu Val Ala Ala Ala Ala Ser
1 5 10 15
Ala Leu Ala Ala Asp Ala Gln Asp Lys Arg Leu Val Lys Thr Phe Val
20 25 30
Pro Gly Ala Tyr Ile Phe Glu Leu Glu Asp Gly His Asp Pro Ala Thr
35 40 45
Phe Glu Gln Thr Val Gly Lys Asp Gly Thr Thr Arg Met Lys Leu Asp
50 55 60
Tyr Glu Leu Phe Lys Gly Val Ser Val Gln Leu His Asp Val Asp Lys
65 70 75 80
Ala His Glu Lys Ala Ala Lys Leu Ala Asp Thr Pro Ala Val Lys Ala
85 90 95
Val Tyr Pro Val Gln Leu Phe Asn Met Pro Lys Pro Lys Val Glu Trp
100 105 110
Ile Ala Lys Asp Gly Thr Lys Ala Pro Gly Gly Leu Leu Ser Ser Arg
115 120 125
Ala Asp Asp Gly Ala Asp Ile Phe Ser Pro His Val Met Thr Gln Val
130 135 140
Asp Lys Leu Arg Ala Lys Gly Val Thr Gly Lys Gly Val Lys Ile Ala
145 150 155 160
Val Val Asp Thr Gly Ile Asp Tyr Lys His Pro Ala Leu Gly Gly Cys
165 170 175
Phe Gly His Gly Cys Leu Val Ala Phe Gly Thr Asp Leu Val Gly Asp
180 185 190
Ala Tyr Asp Gly Ser Asn Thr Pro His Pro Asp Pro Asp Pro Met Asp
195 200 205
Cys Gly Gly His Gly Ser His Val Ala Gly Ile Val Ala Ala Gln Pro
210 215 220
Asn Thr Phe Gly Phe Thr Gly Ala Ala Pro Gly Ala Thr Leu Gly Ala
225 230 235 240
Tyr Arg Val Phe Gly Cys Lys Gly Gln Ala Gly Asn Asp Val Leu Ile
245 250 255
Ala Ala Phe Asn Gln Ala Tyr Gln Asp Gly Ala Asn Ile Ile Thr Ala
260 265 270
Ser Ile Gly Gly Pro Ser Gly Trp Ser Glu Asp Pro Trp Ala Glu Ala
275 280 285
Val Ser Arg Ile Val Asp Lys Gly Val Pro Cys Thr Val Ser Ala Gly
290 295 300
Asn Glu Gly Ala Glu Gly Ile Phe Tyr Ala Ser Thr Ala Ala Asn Gly
305 310 315 320
Arg Arg Val Ser Ala Ile Ala Ser Tyr Asp Asn Val Gln Thr Pro Ser
325 330 335
Leu Ile Tyr Asn Ala Lys Tyr Gln Ile Asp Asn Gly Ala Asp Thr Lys
340 345 350
Phe Gly Tyr Val Pro Ser Glu Pro Ala Ser Trp Asp Gly Val Thr Leu
355 360 365
Pro Ala Trp Ser Asn Ser Leu Asp Pro Thr Ile Pro Asp Asp Gly Cys
370 375 380
Asp Pro Phe Pro Ala Asn Thr Pro Asp Leu Ser Asn Tyr Ile Val Leu
385 390 395 400
Ile Arg Arg Gly Ser Cys Pro Phe Thr Gln Lys Val Asn Asn Ala Val
405 410 415
Ala Lys Gly Ala Lys Tyr Val Ile Val Tyr Asn Asn Asn Ala Val Gly
420 425 430
Ala Ile Pro Met Asp Leu Thr Glu Val Pro Ala Gly Ser Ile Lys Ala
435 440 445
Ala Ser Met Ile Asp Gly Thr Thr Gly Ala Thr Phe Ile Asn Ala Leu
450 455 460
Lys Asp Gly Lys Lys Leu Thr Leu Lys Met Val Ser Pro Gln Lys Thr
465 470 475 480
Asp Ser Glu Val Asn Thr Ser Asn Asn Thr Ile Thr Gly Gly Ala Leu
485 490 495
Ser Thr Phe Thr Ser Trp Gly Pro Thr Trp Glu Met Asp Thr Lys Pro
500 505 510
Gln Phe Gly Ala Val Gly Gly Asn Val Leu Ser Thr Tyr Pro Arg Ala
515 520 525
Leu Gly Ser Tyr Ala Val Leu Ser Gly Thr Ser Met Ser Cys Pro Gln
530 535 540
Thr Ala Gly Ile Ile Ala Leu Ile His Glu Val Arg Gly Thr Tyr Asp
545 550 555 560
Pro Glu Leu Ile Gln Asn Leu Leu Ser Ala Asn Ala Asn Pro Gln Leu
565 570 575
Phe Asn Asp Gly Thr Lys Phe Tyr Asp Phe Leu Ala Pro Val Pro Gln
580 585 590
Gln Gly Gly Gly Leu Ile Gln Ala Tyr Asp Ala Ala Tyr Ala Thr Thr
595 600 605
Leu Leu Ser Pro Ser Ser Leu Ser Phe Asn Asp Thr Asp His Phe Val
610 615 620
Glu Thr Leu Lys Phe Lys Leu Gln Asn Thr Glu Ser Lys Lys Ile Thr
625 630 635 640
Tyr Lys Ile Thr His Thr Pro Ala Ile Thr Met Tyr Ala Leu Gly Asp
645 650 655
Gly Ser Pro Ser Val Gln Pro Phe Pro Asn Glu Ala Val Lys Ala Val
660 665 670
Ala Thr Ile Lys Leu Ser Glu Thr Ser Val Thr Leu Asn Gly Gly Gln
675 680 685
Ser Lys Ser Ile Ser Val Ser Pro Thr Pro Pro Gln Gly Leu Asp Ala
690 695 700
Lys Arg Leu Ala Leu Trp Ser Gly Tyr Ile Val Ile Asn Gly Thr Asp
705 710 715 720
Gly Thr Ser Leu Ser Leu Pro Tyr Gln Gly Leu Thr Gly Ser Leu His
725 730 735
Lys Ser Ala Val Leu Gly Ala Asn Asn Thr Trp Ile Ser Lys Ser Thr
740 745 750
Asp Lys Gln Ser Asn Pro Val Pro Pro Asn Ser Thr Phe Leu Ile Pro
755 760 765
Ala Pro Gly Asn Ala Arg Ser Asn Asp Thr Leu Pro Gln Leu Thr Ala
770 775 780
Ala Leu Tyr Leu Gly Ser Arg Lys Val Arg Ala Asp Ile Val Pro Leu
785 790 795 800
Thr Thr Cys Pro Pro Lys Asn Leu Thr Thr Glu Phe Gln Gly Ile Lys
805 810 815
Thr Ile Gly Gln Pro Tyr Asn Phe Pro Ala Leu Trp Gly Thr Arg Gly
820 825 830
Leu Asn Asn Phe Pro Trp Asp Gly Arg Leu Asp Ser Gly Asn Tyr Ala
835 840 845
Pro Pro Gly Lys Tyr Arg Phe Val Val Arg Ala Leu Arg Ile Phe Gly
850 855 860
Asp Glu Lys Lys Lys Glu Asp Trp Asp Val Ser Thr Ser Pro Ala Leu
865 870 875 880
His Ile Lys Tyr Gln
885
<210> 88
<211> 882
<212> PRT
<213> 尖孢镰刀菌
<400> 88
Met Val Arg Leu Ser Ile Val Ala Ala Leu Tyr Ala Ala Val Val Ser
1 5 10 15
Ala Val Asp Phe Asp Lys Ile Ala Val Gly Lys Gly His Ser Arg Leu
20 25 30
Pro Gly Ala Tyr Ile Phe Glu Phe Glu Glu Asn His Asp Cys Ala Asp
35 40 45
Phe Phe Ala Lys Ala Ser Ser Arg Gly Lys Thr Arg Met Gln Tyr Asn
50 55 60
Tyr Lys Leu Phe Lys Gly Ala Ser Ile Gln Phe Thr Asp Ile Asp Asn
65 70 75 80
Ala Glu Asp Leu Ala Ser Glu Met Ala Leu Leu Pro Gly Ile Lys Gln
85 90 95
Lys Trp Pro Val Gln Thr Phe Ser Ile Pro Lys Pro Glu Val His Trp
100 105 110
Thr Gly Thr Pro Gly Met Glu Tyr Lys Ser Ile Gln Lys Arg Gly Phe
115 120 125
Glu Glu Arg Asp Ala Ala Asn Asp Thr Phe Ser Pro His Val Met Thr
130 135 140
Gln Ile Asp Lys Leu Arg Ala Glu Gly Val Thr Gly Lys Gly Leu Lys
145 150 155 160
Val Ala Leu Val Asp Ser Gly Ile Asp Tyr Lys His Pro Ala Leu Gly
165 170 175
Gly Cys Phe Gly Lys Asn Cys Leu Val Ser Phe Gly Thr Asp Leu Val
180 185 190
Gly Asp Ala Tyr Asp Gly Ser Asn His Ala Lys Pro Asp Asn Asp Pro
195 200 205
Met Asp Cys Ala Gly His Gly Thr His Val Ala Gly Ile Leu Ala Ala
210 215 220
Gln Lys Asn Thr Met Gly Phe Thr Gly Ala Ala Pro Gly Val Gln Ile
225 230 235 240
Gly Ala Tyr Arg Ala Phe Gly Cys Asn Gly Glu Ala Gly Asn Asp Val
245 250 255
Leu Ile Ala Ala Phe Asn Gln Ala Phe Glu Asp Gly Ala Asp Ile Ile
260 265 270
Ser Ala Ser Ile Gly Gly Pro Ser Gly Trp Ser Glu Glu Pro Trp Ala
275 280 285
Val Ala Val Ser Arg Ile Val Glu Gln Gly Val Pro Cys Val Leu Ala
290 295 300
Ala Gly Asn Ala Gly Ala Thr Gly Met Phe Tyr Ala Ser Thr Ala Ala
305 310 315 320
Asn Gly Lys Lys Val Thr Ala Val Gly Ser Phe Asp Asn Thr Lys Ser
325 330 335
Leu Ala Leu Leu Asn Ala Ser Lys Tyr Ser Ile Asp Gly Gly Pro Glu
340 345 350
His Glu Phe Gly His Leu Ser Gly Lys Pro Ser Ala Trp Lys Gly Val
355 360 365
Lys Leu Pro Leu Trp Ala Leu Asn Tyr Asp Thr Ser Val Ala Asp Asp
370 375 380
Gly Cys Asp Glu Trp Pro Lys Asn Thr Pro Asp Leu Gly Asn Tyr Ile
385 390 395 400
Val Leu Leu Arg Arg Gly Ser Cys Thr Phe Asp Ala Lys Ala Ala Asn
405 410 415
Ala Val Lys Ala Gly Ala Arg Tyr Ile Met Phe Tyr Ser Asn Lys Glu
420 425 430
Asp Leu Ala Pro Phe Asp Val Ser Ser Val Gly Gly Ile Lys Ala Ala
435 440 445
Ser Ile Val Ser Pro Glu Gln Gly Ala Glu Trp Ile Ala Ser Leu Lys
450 455 460
Ala Lys Lys Lys Ile Thr Leu Ser Met Ser Asp Gly Ser Asp Gly Asn
465 470 475 480
Val Ile Leu Glu Gln Lys His Asn Asn Ile Thr Ala Gly Ala Ala Ser
485 490 495
Thr Phe Ser Ser Trp Gly Pro Thr Trp Glu Met Asp Val Lys Pro Gln
500 505 510
Phe Gly Ala Pro Gly Gly Lys Ile Leu Ser Thr Tyr Pro Arg Ser Met
515 520 525
Gly Ser Tyr Ala Val Leu Ser Gly Thr Ser Met Ala Ser Pro Leu Val
530 535 540
Ala Gly Ile Val Ala Leu Ile Ala Glu Ala Arg Gly Thr Arg Asp Pro
545 550 555 560
Ala Leu Ile Glu Asn Leu Leu Ser Ala Ser Ala Lys Pro Gln Leu Phe
565 570 575
Asn Asp Gly Lys Thr Phe Tyr Asp Phe Leu Ala Pro Val Pro Gln Gln
580 585 590
Gly Gly Gly Ile Val Gln Ala His Asp Ala Ala His Ala Lys Val Leu
595 600 605
Leu Ser Arg Ser Ser Leu Ser Phe Asn Asp Thr Asp His Phe Val Glu
610 615 620
Ser Leu Asn Phe Thr Val Lys Asn Thr Gly Lys Lys Gln Ile Asp Leu
625 630 635 640
Gln Ile Ser His Val Pro Ala Val Thr Met Phe Thr Leu Val Glu Asn
645 650 655
Tyr Ile Tyr Pro Asp Glu Phe Pro Asn Asp Phe Ala Lys Glu His Ala
660 665 670
Ser Leu Lys Phe Ser Glu Ser Lys Val Thr Ile Asp Ala Gly Glu Ser
675 680 685
Ile Val Ile Glu Val Leu Ala Thr Pro Pro Lys Gly Leu Asn Ala Thr
690 695 700
Arg Leu Pro Val Trp Ser Gly Tyr Val Thr Ile Asn Gly Thr Asp Gly
705 710 715 720
Thr Ser Leu Ser Leu Pro Tyr Gln Gly Leu Thr Gly Ser Leu His Asp
725 730 735
Ser Met Val Leu Gly Pro Gln Asp Thr Trp Ile Ala Asn Ser Thr Asp
740 745 750
Glu Asn Arg Phe Pro Leu Pro Asp Asn Thr Thr Trp Thr Leu Pro Lys
755 760 765
Pro Gly Thr Ala Asn Asn Leu Thr Asp Thr Leu Pro Gly Leu Thr Trp
770 775 780
Phe Leu Ala Leu Gly Ser Ala Lys Leu His Ala Glu Ile Met Pro Val
785 790 795 800
Thr Thr Glu Lys Pro Thr Ser Ser Lys Lys Pro Arg Val Ile Gly Glu
805 810 815
Pro Ile Asp Phe Pro Leu Leu Trp Asn Ala Met Gly Ala Asn Ser Gln
820 825 830
Ala Phe Thr Gly Glu Leu Ala Asp Gly Thr Phe Ala Pro Ala Gly Gln
835 840 845
Tyr Val Val Arg Tyr Arg Ala Leu Arg Ile Phe Gly Asn Glu Lys Lys
850 855 860
Lys Glu Asp Trp Asp Glu Ala Tyr Ser Pro Val Phe Thr Ile Arg Tyr
865 870 875 880
Glu Lys
<210> 89
<211> 875
<212> PRT
<213> 玉米赤霉
<400> 89
Met Val Arg Phe Gly Leu Ser Ala Leu Leu Phe Ala Gly Leu Ser Leu
1 5 10 15
Ala Gln Asp Glu Leu Ser Gln Pro Pro Gln Pro Glu Gln Gly Thr Val
20 25 30
Ala His Leu Pro Gly Ala Tyr Ile Phe Glu Phe Glu Glu Ser Gln Asp
35 40 45
Thr Ser Val Phe Tyr Asp Lys Thr Glu Ser Gln Ser Thr Thr Arg Val
50 55 60
Lys Phe Asp Tyr Asp Leu Phe Lys Gly Val Ser Val Gln Phe Asp Asp
65 70 75 80
Val Ala Ser Ala Glu Glu Leu Ala Ala Lys Met Ala Ala Leu Pro Val
85 90 95
Val Lys Asn Val Trp Pro Val Lys Val Tyr Gly Ile Pro Ala Pro Arg
100 105 110
Val Glu Trp Val Ala Glu Pro Gly Met Lys Ala Pro Leu Ser Lys Arg
115 120 125
Ala Val Asn Asp Thr Ala Asp Thr Phe Ser Pro His Val Met Thr Gln
130 135 140
Val Asp Lys Leu Arg Lys Lys Gly Ile Thr Gly His Gly Ile Lys Val
145 150 155 160
Ala Val Ile Asp Thr Gly Ile Asp Tyr Lys His Pro Ala Leu Gly Gly
165 170 175
Cys Phe Gly Pro Asp Cys Leu Val Ser Phe Gly Thr Asp Leu Val Gly
180 185 190
Asp Asp Tyr Asp Gly Phe Asn Ala Val His Pro Asp Asp Asp Pro Met
195 200 205
Asp Cys Ala Gly His Gly Thr His Val Ala Gly Ile Val Ala Ala Gln
210 215 220
Thr Asn Pro Phe Gly Phe Thr Gly Thr Ala Pro Gly Val Thr Leu Gly
225 230 235 240
Ala Tyr Arg Val Phe Gly Cys Ser Gly Glu Ala Gly Asn Asp Val Leu
245 250 255
Ile Ala Ala Tyr Asn Gln Ala Tyr Gln Asp Gly Ala Asp Ile Ile Thr
260 265 270
Ala Ser Ile Gly Gly Pro Ser Gly Trp Ser Glu Glu Pro Trp Ala Val
275 280 285
Ala Val Ser Arg Ile Val Glu Lys Gly Val Pro Cys Pro Val Ser Ala
290 295 300
Gly Asn Ser Gly Asp Val Gly Met Phe Tyr Ala Ser Thr Ala Ala Asn
305 310 315 320
Gly Asn Lys Val Met Ala Ile Ala Ser Tyr Asp Asn Ser Asp Tyr Ile
325 330 335
Ser Leu Leu Lys Gln His His Tyr Thr Val Asp Gly Ser Asp Thr Lys
340 345 350
Ile Ala Phe Gly Met Thr Ala Gly Thr Pro Ala Ala Trp Asp Asp Val
355 360 365
Lys Leu Pro Ile Trp Ala Pro Ser Leu Asp Thr Thr Val Ala Asn Gly
370 375 380
Gly Cys Asp Pro Tyr Pro Ala Asp Thr Pro Asp Leu Ser Lys Phe Ile
385 390 395 400
Val Leu Val Arg Arg Gly Thr Cys Thr Phe Ala Gln Lys Ala Gln Asn
405 410 415
Ala Ala Asn Ala Gly Ala Lys Tyr Phe Ile Val Tyr Asn Asn Ala Gly
420 425 430
Gly Ala Asn Glu Ile Asp Val Ser Ala Val Lys Ser Ile Leu Ala Ala
435 440 445
Gly Met Val Thr Ser Lys Val Gly Ala Lys Trp Ile Gly Leu Leu Lys
450 455 460
Glu Gly Ser Lys Val Thr Leu Glu Met Ser Ala Gly Ser Asp Gly Glu
465 470 475 480
Val Ile Met Glu Glu Ser Lys Asn Asp Lys Thr Gly Gly Ala Val Ser
485 490 495
Thr Tyr Ser Ser Trp Gly Pro Thr Trp Glu Met Asp Val Lys Pro Gln
500 505 510
Phe Gly Ser Pro Gly Gly Asn Ile Leu Ser Thr Tyr Pro Leu Ala Lys
515 520 525
Gly Gly Tyr Ala Val Leu Ser Gly Thr Ser Met Ala Cys Pro Leu Val
530 535 540
Ala Ala Thr Ile Ala Leu Ile Ala Glu Val Arg Gly Thr Leu Asp Pro
545 550 555 560
Glu Thr Ile Glu Asn Leu Leu Ala Ser Thr Ser Asn Pro Gln Leu Phe
565 570 575
Asn Asp Gly Thr Gly Phe Ser Asp Tyr Leu Ala Pro Val Pro Gln Gln
580 585 590
Gly Gly Gly Met Ile Gln Ala Tyr Asp Ala Ala Tyr Ser Thr Val Leu
595 600 605
Met Asp Val Ser Ser Leu Ser Phe Asn Asp Thr Asp Asn Phe Ile Lys
610 615 620
Val Arg Asn Phe Thr Ile His Asn Thr Gly Lys Lys Glu Ile Asp Leu
625 630 635 640
Ser Val Ser His Ile Pro Thr Lys Ser Val Tyr Thr Leu Asp Lys Asp
645 650 655
Ser Ile Tyr Ala Ser Thr Phe Pro Asn Asp Val Ala Asp Ala His Ala
660 665 670
Glu Leu Lys Phe Ser Glu Ala Lys Leu Ser Val Gly Ala Gly Glu Lys
675 680 685
Val Thr Ile Glu Val Val Pro Ser Ala Pro Glu Gly Leu Asp Ala Lys
690 695 700
Arg Leu Ala Leu Trp Ser Gly Tyr Ile Ala Ile Asn Gly Thr Gly Val
705 710 715 720
Ser Leu Ser Leu Pro Tyr Gln Gly Met Thr Gly Ser Leu His Asp Ser
725 730 735
Val Val Leu Gly Ser Asp Asp Thr Trp Ile Ala Arg Ser Asn Asp Lys
740 745 750
Asp Lys Leu Thr Pro Val Pro Ala Asn Thr Thr Phe Ile Leu Pro Val
755 760 765
Lys Gly Gln Asn Ala Thr Asp Ala Gln Pro Ala Pro Ala Leu Ala Trp
770 775 780
Lys Leu Ala Leu Gly Ser Ala Lys Leu Glu Ala Glu Leu Ile Pro Val
785 790 795 800
Gly Gly Asn Ser Thr Lys Ser Leu Gly Ala Pro Ala Gly Phe Pro Val
805 810 815
Gln Trp Asn Pro Met Gly Asp Gly Asn Ile Val Trp Asn Gly Lys Leu
820 825 830
Ala Asp Gly Gly Tyr Ala Pro Ala Gly Lys Tyr Lys Val Lys Tyr Gln
835 840 845
Ala Leu Arg Ile Phe Gly Asp Glu Lys Val Lys Ser Asp Trp Asp His
850 855 860
Ser Glu Ser Pro Val Phe Ser Val Lys Tyr Pro
865 870 875
<210> 90
<211> 906
<212> PRT
<213> Epichloe festucae
<400> 90
Met Val Arg Leu Arg Thr Ser Phe Val Ala Ser Leu Leu Ala Ala Ala
1 5 10 15
Ala Ser Thr Leu Ala Ala Asp Pro Asn Glu Lys Val Ala Asn Thr Val
20 25 30
Val Pro Gly Ala Tyr Ile Phe Glu Leu Ala Asp Gly Gln Asp Ala Ala
35 40 45
Ala Leu Glu Lys Ala Val Gly Lys Asp Gly Lys Thr Arg Met Arg Leu
50 55 60
Asp Phe Asp Leu Phe Lys Ala Val Ser Val Gln Leu His Asp Val Asp
65 70 75 80
Lys Ala Lys Glu Lys Ala Thr Lys Leu Ala Ala Leu Pro Gln Val Lys
85 90 95
Ser Val Tyr Pro Val Thr Leu Tyr Lys Arg Pro Asn Pro Lys Ile Glu
100 105 110
Trp Ile Ala Gln Lys Gly Ser Thr Met Gln Lys Ala Ala Leu Thr Ser
115 120 125
Arg Ala Asp Gly Pro Glu Gly Pro Asp Gly Pro Glu Gly Ala His Ala
130 135 140
Asp Thr Asp Thr Tyr Ser Pro His Val Met Thr Gln Val Asp Lys Leu
145 150 155 160
Arg Ala Lys Gly Ile Thr Gly Lys Arg Val Lys Ile Ala Leu Val Asp
165 170 175
Thr Gly Ile Asp Phe Gly His Pro Ala Leu Gly Gly Cys Phe Gly Asp
180 185 190
Gly Cys Leu Val Ser Phe Gly Thr Asp Leu Val Gly Asp Asp Phe Asn
195 200 205
Gly Phe Asn Thr Pro His Pro Asp Asn Ile Pro Lys Asp Cys Ser Gly
210 215 220
His Gly Thr His Val Ala Gly Ile Ile Ala Ala Gln Asn Asn Thr Tyr
225 230 235 240
Gly Phe Thr Gly Thr Ala Pro Asp Ala Thr Leu Gly Ile Tyr Arg Val
245 250 255
Phe Gly Cys Thr Gly Glu Ser Thr Asn Glu Ile Leu Ile Ala Ala Phe
260 265 270
Asn Met Ala Tyr Gln Gly Gly Ala Asn Ile Ile Thr Ala Ser Val Gly
275 280 285
Ser Ala Ser Gly Trp Ser Glu Asp Pro Trp Ala Glu Ala Val Ser Arg
290 295 300
Ile Val Gln Lys Gly Val Pro Cys Val Leu Ser Ala Gly Asn Asp Gly
305 310 315 320
Asp Gln Gly Ile Phe Tyr Ala Ser Ala Ala Ala Asp Gly His Arg Val
325 330 335
Ala Ala Ile Ala Ala Phe Asp Asn Val Gln Thr Pro Thr Val Tyr Ser
340 345 350
Asn Ser Lys Tyr Glu Ile Asp Asp Gly Pro Asp Lys Val Phe Gly Tyr
355 360 365
Leu Ala Ser Lys Pro Ala Asn Trp Asp Gly Val Thr Leu Pro Ala Trp
370 375 380
Ala Asn Ser Leu Asp Pro Thr Val Val Asn Asp Gly Cys Gln Pro Phe
385 390 395 400
Pro Ala Asn Thr Pro Asp Leu Ser Lys Tyr Ile Val Leu Ile Arg Arg
405 410 415
Gly Gly Cys Asn Phe Asp Lys Lys Val Gly Asn Ala Ile Ala Lys Gly
420 425 430
Ala Lys Asn Val Ile Val Tyr Asn Asn Ala Ala Ala Gly Ala Ala Val
435 440 445
Met Asp Leu Asn Ser Leu Arg Pro Gly Ser Leu Asn Ala Ala Ser Met
450 455 460
Ile Asp Ala Asp Thr Gly Ala Thr Phe Ile Lys Ala Leu Lys Glu Gly
465 470 475 480
Lys Lys Leu Thr Leu Lys Met Ser Ser Pro Gly Lys Thr Gly Ser Thr
485 490 495
Leu Arg Thr Phe Lys Asn Thr Ala Thr Gly Gly Ala Leu Ser Ser Thr
500 505 510
Thr Ser Trp Gly Pro Thr Trp Glu Met Asp Leu Lys Pro Gln Phe Gly
515 520 525
Ala Val Gly Gly Asn Ile Leu Ser Thr Phe Pro Val Ala Leu Gly Ser
530 535 540
Tyr Ala Ala Leu Ser Gly Thr Ser Met Ala Cys Pro Gln Val Ala Gly
545 550 555 560
Ile Met Ala Leu Ile His Gln Val Arg Gly Thr Tyr Asp Ala Glu Leu
565 570 575
Ile Gln Asn Leu Leu Ser Gly Asn Ser Asn Pro Gln Leu Phe Asn Asp
580 585 590
Gly Thr Lys Phe Tyr Asp Phe Leu Ala Pro Val Pro Gln Gln Gly Ala
595 600 605
Gly Leu Ile Gln Ala Tyr Asp Ala Ala Tyr Ala Thr Thr Val Leu Ser
610 615 620
Pro Ser Gly Leu Ser Phe Asn Asp Thr Asp His Phe Ala Lys Arg Leu
625 630 635 640
Glu Phe Lys Leu Lys Asn Thr Asp Lys Lys Arg Ala Thr Tyr Arg Val
645 650 655
Thr His Val Pro Thr Ile Thr Met Tyr Thr Leu Ala Lys Asp Ser Ile
660 665 670
Tyr Ala Gln Lys Phe Pro Asn Asp His Val Asp Ala Ala Ala Thr Leu
675 680 685
Lys Phe Ser Lys Thr Ser Ile Thr Leu Arg Gly Gly Glu Ala Glu Thr
690 695 700
Val Thr Val Ser Ala Thr Pro Pro Glu Gly Leu Asp Thr Lys Arg Leu
705 710 715 720
Ala Leu Trp Ser Gly Tyr Ile Val Ile Asn Gly Thr Asp Gly Ala Ser
725 730 735
Leu Ser Leu Pro Tyr Gln Gly Leu Thr Gly Ser Leu Arg Asp Ser Thr
740 745 750
Val Leu Gly Pro Ser Asp Thr Trp Ile Ser Lys Ser Ile Asp Glu Lys
755 760 765
Ala Arg Lys Val Pro Pro Asn Ser Val Phe Val Ile Pro Gly Pro Gly
770 775 780
Asn Ala Gly Val Asn Asp Thr Leu Pro Val Leu Ser Ala Asn Leu Ala
785 790 795 800
Leu Gly Ser Ile Lys Val Arg Ala Asp Ile Val Pro Leu Thr Pro Cys
805 810 815
Arg Pro Asn Asp Met Ile Pro Cys Pro Pro Lys Asp Met Met Thr Glu
820 825 830
Phe Trp Gly Val Lys Thr Ile Gly Gln Pro Tyr Arg Phe Pro Ala Gln
835 840 845
Trp Ser Ser Arg Gly Val Asn Asn Trp Ala Trp Asp Gly Arg Leu Asp
850 855 860
Ser Gly Asn Tyr Ala Pro Pro Gly Lys Tyr Gln Phe Val Val Arg Ala
865 870 875 880
Leu Arg Ile Phe Gly Asp Glu Arg Lys Lys Glu Asp Trp Asp Val Ser
885 890 895
Thr Ser Pro Ala Leu Ile Ile Glu Tyr Gln
900 905
<210> 91
<211> 881
<212> PRT
<213> Nectria haematococca
<400> 91
Met Arg Leu Tyr Ser Leu Val Trp Leu Phe Ala Ala Ala Ala Ser Ala
1 5 10 15
Val Asp Ile Lys His Ile Gly Ile Lys Lys Thr Ser Ser Arg Leu Pro
20 25 30
Gly Ala Tyr Ile Phe Glu Phe Glu Asp Asp Asn Asp Cys Ser Asp Phe
35 40 45
Tyr Ala Lys Ala Ser Glu His Gly Thr Thr Arg Met Gln Tyr Asp Tyr
50 55 60
Lys Leu Phe Lys Gly Ala Ser Ile Gln Phe Asn Asp Val Asp His Ala
65 70 75 80
Glu Asp Leu Ala Val Met Met Thr Lys Met Pro Ala Val Lys Arg Ser
85 90 95
Trp Pro Val Lys Met Ile Ser Leu Pro Thr Pro Glu Val His Trp Gln
100 105 110
Gly Thr Pro Gly Lys Asp Tyr Ser Ala Ile Arg Arg Arg Asp Leu Tyr
115 120 125
Gly Arg Asp Thr Thr Asn Asp Thr Phe Ser Pro His Ile Met Thr Gln
130 135 140
Val Asp Lys Leu Arg Ala Glu Gly Ala Thr Gly Lys Gly Ile Lys Val
145 150 155 160
Ala Leu Val Asp Ser Gly Val Asp Tyr Thr His Pro Ala Leu Gly Gly
165 170 175
Cys Phe Gly Pro Gly Cys Leu Val Ser Phe Gly Thr Asp Leu Val Gly
180 185 190
Asp Thr Tyr Asn Gly Lys Asn Ala Ala Thr Pro Asp Asp Asp Pro Gln
195 200 205
Asp Cys Gln Gly His Gly Thr His Thr Ala Gly Thr Leu Ala Ala Gln
210 215 220
Ala Asn Ser Met Gly Phe Thr Gly Ala Ala Pro Gly Val Thr Ile Gly
225 230 235 240
Ala Tyr Arg Ala Phe Gly Cys Lys Gly Glu Ser Gly Asn Asp Ile Leu
245 250 255
Ile Ala Ala Phe Asn Arg Ala Phe Glu Asp Gly Ala Asp Ile Ile Ser
260 265 270
Ala Ser Ile Gly Gly Pro Ala Gly Trp Ser Glu Asp Pro Trp Ala Val
275 280 285
Ala Val Ser Arg Ile Val Asp Gln Gly Val Pro Cys Ile Leu Ser Ala
290 295 300
Gly Asn Ala Gly Glu Ala Gly Leu Phe Tyr Ala Asn Thr Ala Gly Asn
305 310 315 320
Gly Lys His Val Thr Ala Val Ala Ser Phe Asp Asn Thr Lys Ser Leu
325 330 335
Ala Leu Phe Asn Val Ser His Tyr Thr Val Asn Asp Gly Pro Lys Asn
340 345 350
Glu Phe Gly Ser Leu Ala Gly Ala Pro Asp Ala Trp Lys Gly Val Lys
355 360 365
Leu Pro Leu Trp Ala Leu Asn Tyr Asn Thr Thr Val Ala Asp Asp Ala
370 375 380
Cys Ser Pro Leu Pro Ala Asn Thr Pro Asp Leu Ser Lys Tyr Ile Val
385 390 395 400
Leu Ile Arg Arg Gly Thr Cys Thr Phe Glu Val Lys Ala Thr Asn Leu
405 410 415
Ala Lys Phe Gly Ala Gln Tyr Met Met Ile Tyr Ser Asp Leu Glu Asn
420 425 430
Leu Met Pro Tyr Asp Val Ser Met Val Ala Gly Ile Lys Ala Ala Ala
435 440 445
Ile Val Ser Pro Ser Gln Gly Leu Glu Trp Met Lys His Leu Ser Lys
450 455 460
Gly Glu Lys Val Val Leu Glu Met Ser Asp Gly Ser Asp Gly Asn Val
465 470 475 480
Met Leu Gln Glu Thr Val Asn Asn Ile Thr Ala Gly Ala Val Ser Ala
485 490 495
Phe Thr Ser Trp Gly Pro Thr Trp Glu Met Asp Val Lys Pro Gln Phe
500 505 510
Gly Ala Pro Gly Gly Glu Ile Leu Ser Thr Tyr Pro Arg Asp Gln Gly
515 520 525
Ser Phe Ala Val Leu Ser Gly Thr Ser Met Ala Thr Pro Leu Leu Ala
530 535 540
Gly Ile Val Ala Leu Ile Ala Glu Val Arg Gly Thr Leu Asp Pro Ile
545 550 555 560
Phe Leu Glu Asn Leu Leu Ser Ser Thr Ser Lys Pro Gln Leu Phe Asn
565 570 575
Asp Gly Lys Ala Phe Tyr Arg Phe Leu Ala Pro Val Pro Gln Gln Gly
580 585 590
Ser Gly Ile Val Gln Ala His Asp Ala Ala His Ser Lys Ile Ile Leu
595 600 605
Ser Pro Ser Ser Leu Ser Phe Asn Asp Thr Asp His Phe Val Glu Ser
610 615 620
Leu Asn Phe Thr Leu Lys Asn Thr Gly Ser Glu Glu Val Glu Phe Gln
625 630 635 640
Ile Ser His Val Pro Ala Val Thr Met Tyr Thr Leu Ala Lys Gly Tyr
645 650 655
Met Tyr Pro Asp Gly Phe Pro Asn Asp Phe Ala Asn Glu Gln Ala Ser
660 665 670
Val Arg Phe Ser Glu Ser Lys Val Ser Ile Ser Pro Gly Asp Ser Ile
675 680 685
Val Ile Glu Val Leu Pro Thr Pro Pro Gln Gly Val Asp Ala Lys Arg
690 695 700
Leu Pro Val Trp Ser Gly Tyr Val Ala Ile Asn Gly Thr Asp Gly Ser
705 710 715 720
Ala Leu Ser Leu Pro Tyr Gln Gly Leu Thr Gly Ser Leu His Asp Ser
725 730 735
Pro Val Leu Gly Pro Asn Asp Thr Trp Ile Thr Ser Ser Thr Asp Glu
740 745 750
Asn Phe Phe Pro Ala Lys Ala Asn Lys Thr Trp Ile Leu Pro Lys Ala
755 760 765
Gly Thr Ala Asn Asn Gln Thr Asp Glu Leu Pro Ala Val Val Trp Phe
770 775 780
Leu Ala Leu Gly Thr Ala Lys Leu His Gly Asp Ile Ile Pro Val Thr
785 790 795 800
Thr Asp Lys Ser Ser Ser Ser Gly Lys Ala Arg Val Ile Gly Glu Pro
805 810 815
Val Asn Phe Pro Leu Leu Trp Asn Pro Met Gly Arg Asn Ser Gln Pro
820 825 830
Phe Thr Gly Glu Leu Ala Gly Gly Gly Phe Ala Pro Ala Gly Met Tyr
835 840 845
Val Val Arg Tyr Arg Ala Leu Arg Ile Phe Gly Asn Glu Asn Asp Glu
850 855 860
Lys Asp Trp Asp Glu Ser Tyr Ser Pro Pro Phe Ala Ile Gln Tyr Ala
865 870 875 880
Gln
<210> 92
<211> 874
<212> PRT
<213> Sordaria macrospora
<400> 92
Met Val Arg Leu Gly Leu Ala Thr Thr Leu Leu Ala Ala Ala Ser Phe
1 5 10 15
Ala Gln Ala Ala His His Lys Ala Pro Lys Val Val Pro Gly Ala Tyr
20 25 30
Ile Val Glu Tyr Glu Asp Ser His Ala Ala Ser Ile Leu Asn Ser Ile
35 40 45
Lys Gly Asp Ala Thr Ile Arg Lys Asp Ile Arg His Glu Leu Phe Lys
50 55 60
Gly Ala Ser Phe Gln Phe Lys Asp Leu Asp Lys Ala Glu Asp Leu Ala
65 70 75 80
Gly Lys Ile Ala Ala Met Ser Gly Val Lys Ala Met Tyr Pro Val Arg
85 90 95
Arg Tyr Ser Val Pro Glu His Thr Val His Tyr Thr Gly Ser Ser Val
100 105 110
Gln Glu Val Val Ala Lys Arg Asp Thr Ala Lys Asp Thr Phe Thr Pro
115 120 125
His Leu Met Thr Gln Val Asn Lys Phe Arg Asp Ser Gly Ile Thr Gly
130 135 140
Lys Gly Ile Lys Val Ala Val Ile Asp Thr Gly Ile Asp Tyr Leu His
145 150 155 160
Pro Ala Leu Gly Gly Cys Phe Gly Ala Gly Cys Leu Val Ser Tyr Gly
165 170 175
Ala Asp Leu Val Gly Asp Asp Phe Asn Gly Ser Asn Thr Pro Val Pro
180 185 190
Asp Pro Asp Pro Met Asp Cys Gln Gly His Gly Thr His Val Ser Gly
195 200 205
Ile Val Ala Ser Lys Thr Asn Asn Pro Tyr Gly Ile Ile Gly Ala Ala
210 215 220
Ser Asp Val Thr Leu Gly Ala Tyr Arg Val Phe Gly Cys Asp Gly Asp
225 230 235 240
Val Gly Asn Asp Ile Leu Ile Glu Ala Tyr Leu Lys Ala Tyr Asp Asp
245 250 255
Gly Ser Asp Ile Ile Thr Ala Ser Ile Gly Gly Ala Ser Gly Trp Pro
260 265 270
Glu Asp Ser Trp Ala Ala Val Val Ser Arg Ile Val Glu Lys Gly Val
275 280 285
Pro Cys Leu Val Ser Ala Gly Asn Ser Gly Asp Glu Gly Ile Phe Tyr
290 295 300
Ala Ser Thr Ala Ala Asn Gly Lys Arg Val Thr Ala Val Gly Ser Val
305 310 315 320
Asp Asn Ile Ile Ala Pro Ala Leu Leu Ser Glu Gly Thr Tyr Ser Val
325 330 335
Ala Gly Ser Ser Ala Ser Ala Phe Gly Phe Thr Ala Gly Thr Pro Ser
340 345 350
Ala Trp Ala Asn Val Ser Leu Pro Val Trp Ser Val Asn Phe Asp Thr
355 360 365
Thr Asp Ala Ala Asn Gly Cys Glu Ala Phe Pro Asp Asn Thr Pro Asp
370 375 380
Leu Ser Lys Tyr Ile Thr Leu Ile Arg Arg Gly Ser Cys Thr Phe Val
385 390 395 400
Gln Lys Ala Gln Asn Ala Ala Ala Lys Gly Ala Lys Tyr Ile Ile Phe
405 410 415
Tyr Asn Asn Ala Ala Gly Ser Thr Lys Val Asp Ile Ser Ala Val Ala
420 425 430
Gly Ile Gln Gly Ser Ala Met Val Thr Ser Glu Thr Gly Ala Ala Trp
435 440 445
Ile Lys Ala Leu Gln Ala Gly Ser Glu Val Ile Val Asn Met Ala Asp
450 455 460
Pro Glu Thr Ala Pro Lys Asn Leu Ser Asn Phe Pro Asn Pro Asn Thr
465 470 475 480
Ala Gly Phe Leu Ser Thr Tyr Thr Ser Trp Gly Pro Thr Tyr Glu Ile
485 490 495
Asp Val Lys Pro Gln Ile Ser Ala Pro Gly Gly Met Ile Leu Ser Thr
500 505 510
Tyr Pro Arg Ala Leu Gly Ser Tyr Ala Val Leu Ser Gly Thr Ser Met
515 520 525
Ala Cys Pro Leu Ala Ala Ala Thr Trp Ala Leu Val Met Gln Lys Arg
530 535 540
Gly Thr Lys Asp Pro Arg Val Leu Glu Asn Leu Phe Ser Ala Thr Ala
545 550 555 560
His Pro Asn Leu Phe Asn Asp Gly Thr Thr Thr Tyr Pro Met Leu Ala
565 570 575
Pro Val Ala Gln Gln Gly Ala Gly Leu Ile Gln Ala Trp Asp Ala Ala
580 585 590
Asn Ala Asn Ala Leu Leu Ser Ile Ser Ser Ile Ser Phe Asn Asp Thr
595 600 605
Ala Asn Ile Lys Pro Leu Gln Ser Phe Glu Val Thr Asn Thr Gly Lys
610 615 620
Lys Ala Val Thr Tyr Gln Leu Gly His Thr Ser Ala Ala Thr Ala Tyr
625 630 635 640
Thr Phe Ala Asp Ala Asn Thr Ile Asp Pro Ala Asp Phe Pro Asn Glu
645 650 655
Leu Val Ala Asn Lys Ala Thr Leu Val Ile Thr Pro Ala Lys Leu Thr
660 665 670
Leu Asn Pro Gly Gln Lys Lys Thr Val Thr Val Leu Ala Ile Pro Pro
675 680 685
Lys Gly Leu Asp Ala Lys Arg Leu Pro Val Tyr Ser Gly Tyr Ile Thr
690 695 700
Leu Asn Gly Thr Asp Ser Thr Gly Tyr Ser Leu Pro Tyr Gln Gly Val
705 710 715 720
Val Gly Ser Met Lys Ala Val Thr Val Leu Asp Lys Gln Tyr Ser Tyr
725 730 735
Leu Ser Gln Ser Ser Asp Ala Ala Leu Ala Pro Val Ala Ala Gly Thr
740 745 750
Val Phe Thr Leu Pro Pro Ala Gly Lys Ala Asn Glu Thr Gln Tyr Ala
755 760 765
Asn Thr Val Tyr Pro Thr Val Val Leu Thr Leu Ala Met Gly Ser Ala
770 775 780
Glu Val Arg Ala Asp Val Val Asn Ala Lys Gly Lys Thr Ile Gly Gln
785 790 795 800
Val Leu Thr Phe Pro Ala Tyr Trp Asn Pro Arg Gly Thr Phe Glu Trp
805 810 815
Asn Trp Asp Gly Ala Leu Ser Asp Gly Ser Tyr Ala Pro Ala Gly Thr
820 825 830
Tyr Lys Ile Thr Leu Arg Ala Leu Lys Ile Tyr Gly Asn Ser Arg Lys
835 840 845
Pro Gln Asp Trp Glu Thr Gln Thr Thr Glu Ser Phe Ser Ile Lys Tyr
850 855 860
Ala Lys Lys Asn Lys Arg Glu Phe Thr Ala
865 870
<210> 93
<211> 886
<212> PRT
<213> Glomerella graminicola
<400> 93
Met Val Arg Ser Ser Leu Leu Leu Pro Leu Val Ala Ala Ala Ser Ala
1 5 10 15
Val Gln Ala Tyr Val Gln Ser Pro Lys Thr Val Ala Gly Ala Phe Ile
20 25 30
Val Glu Leu Glu Asp Gly Gln Asp Ala Ser Glu Phe Leu Ala Lys Thr
35 40 45
Lys Ala Asp Ala Val Thr Arg Met Asn Leu Asn Phe Thr Leu Phe Lys
50 55 60
Gly Ala Ser Ile Gln Phe Asn Asp Leu Asp Thr Ala Asp Ala Lys Ala
65 70 75 80
Ala Gln Leu Ala Gln Leu Pro Ser Val Lys Asn Met Trp Pro Val Arg
85 90 95
Thr Tyr Gly Ile Pro Asp Ser Glu Val Val Trp Thr Gly Thr Gln Gly
100 105 110
Arg Asp Tyr Val Asp Val Lys Lys Arg Gln Leu Gly Asn Asp Thr Phe
115 120 125
Ser Thr His Val Gln Thr Gln Val Asp Lys Leu His Arg Asn Gly Val
130 135 140
Thr Gly Lys Gly Ile Lys Val Ala Val Ile Asp Thr Gly Ile Asp Tyr
145 150 155 160
Leu His Pro Ala Leu Gly Gly Cys Phe Gly Pro Gly Cys Leu Val Ser
165 170 175
Tyr Gly Thr Asp Leu Val Gly Asn Asn Tyr Asp Gly Phe Asn Glu Gln
180 185 190
Val Pro Asp Pro Asp Pro Met Asp Cys Gly Gly His Gly Thr His Val
195 200 205
Ala Gly Ile Ile Ala Ala Gln Ser Asn Pro Phe Gly Phe Leu Gly Thr
210 215 220
Ala Pro Gly Val Thr Leu Gly Ala Tyr Lys Val Phe Gly Cys Glu Gly
225 230 235 240
Gln Val Gly Asn Asp Val Leu Ile Ala Ala Tyr Asn Arg Ala Phe Glu
245 250 255
Glu Gly Ser Asn Ile Ile Thr Ala Ser Ile Gly Gly Pro Ser Gly Trp
260 265 270
Thr Asp Glu Pro Trp Ala Ala Thr Val Ser Arg Ile Val Asp Ala Gly
275 280 285
Val Pro Cys Thr Val Ser Ala Gly Asn Ser Gly Asp Gln Gly Ile Phe
290 295 300
Phe Ala Ser Thr Ala Ala Ser Gly Arg His Val Thr Ala Ile Ala Ser
305 310 315 320
Phe Asp Asn Ala Val Thr Pro Ala Leu Leu Ile Glu Asn Thr Phe Thr
325 330 335
Val Ala Gly Gly Ala Pro Ser Thr Phe Ala Tyr Thr Pro Gly Glu Pro
340 345 350
Ser Glu Trp Ala Gly Val Glu Leu Pro Leu Trp Ala Ile Thr Thr Asp
355 360 365
Ser Ser Ser Asp Ala Ala Leu Gly Cys Asp Pro Tyr Pro Ala Gly Thr
370 375 380
Pro Asp Leu Ser Gly Lys Ile Val Leu Val Lys Arg Gly Val Cys Thr
385 390 395 400
Phe Val Val Lys Ala Gln Lys Ala Ala Ala Ala Gly Ala Lys Tyr Ile
405 410 415
Met Val Tyr Asn Asn Val Pro Gly Ala Gly Ser Val Ser Val Thr Ala
420 425 430
Val Pro Glu Ile Leu Ala Ile Gly Met Thr Thr Pro Asp Gln Gly Ala
435 440 445
Ala Trp Leu Lys Ser Ile Ala Ala Gly Ser Glu Val Val Leu Lys Met
450 455 460
Thr Asp Pro Glu Lys Ala Glu Gln Ile Leu Thr Gln Ser Val Asn Thr
465 470 475 480
Ala Thr Gly Gly Tyr Val Ser Ser Tyr Ser Ser Trp Gly Pro Asn Trp
485 490 495
Asp Gly Gln Gln Lys Pro Gln Phe Gly Ala Val Gly Gly Asn Val Leu
500 505 510
Ser Thr Tyr Pro Arg Ala Leu Gly Ser Tyr Ala Val Leu Ser Gly Thr
515 520 525
Ser Met Ser Cys Pro Gln Thr Ala Gly Ile Val Ala Leu Ile Ala Glu
530 535 540
Val Arg Lys Thr Leu Asp Pro Ala Thr Ile Glu Asn Leu Leu Ser Ala
545 550 555 560
His Ala Asn Pro Val Asn Phe Asn Ser Gly Ser Gly Pro Glu Gly Leu
565 570 575
Leu Ala Pro Val Ser Gln Gln Gly Ala Gly Leu Val Gln Ala Tyr Asp
580 585 590
Ala Ala Tyr Ser Thr Ile Leu Leu Ser His Ser Ser Leu Ser Phe Asn
595 600 605
Asp Thr Asp Asn Phe Ile Pro Glu Leu Asn Phe Thr Ile Lys Asn Thr
610 615 620
Gly Ser Ala Asp Val Ser Phe Asp Leu Ser Asn Val Gly Ala Ala Thr
625 630 635 640
Ala Tyr Thr Phe Glu Thr Ala Gly Asp Leu Thr Pro Ala Leu Phe Pro
645 650 655
Asn Asn Leu Thr Ala Glu His Ala Thr Val Ala Phe Ser Glu Ser Lys
660 665 670
Val Ser Val Pro Ala Gly Gly Glu Ala Thr Ile Leu Val Thr Pro Thr
675 680 685
Pro Pro Thr Leu Asp Ala Ala Arg Leu Pro Val Trp Ser Gly Tyr Ile
690 695 700
Ala Ile Asn Gly Ser Asp Gly Ser Ser Met Ser Leu Pro Tyr Met Gly
705 710 715 720
Ile Gly Gly Ser Leu His Ser His Val Thr Met Asp Glu Ala Leu Met
725 730 735
Thr Thr Ser Thr Ser Ala Thr Ala Ala Glu Tyr Ala Pro Val Ala Ser
740 745 750
Asn Tyr Thr Tyr Val Leu Pro Pro Pro Gly Thr Ala Asn Lys Thr Asp
755 760 765
Ala Val Val Pro Ala Leu Ala Ile Asp Met Val Phe Gly Ser Ala Leu
770 775 780
Val Arg Ala Asp Leu Val Pro Leu Thr Thr Tyr Pro Pro Asn Ile Thr
785 790 795 800
His Lys Val Phe Gly Thr Lys Thr Leu Gly Gln Pro Arg Ser Phe Pro
805 810 815
Tyr Thr Tyr Thr Ser Arg Gly Val Phe Ala Val Asn Trp Asp Gly Gln
820 825 830
Met Asp Asp Gly Ser Tyr Ala Pro Ala Gly Lys Tyr Lys Phe Ser Val
835 840 845
Lys Ser Leu Arg Val Phe Gly Asp Ala Ser Lys Leu Glu Glu Tyr Asp
850 855 860
Val Ala Glu Thr Gln Pro Phe Arg Ile Ile Tyr Gly Ala Ala Asn Ala
865 870 875 880
Thr Ala Pro Ala Arg His
885
<210> 94
<211> 876
<212> PRT
<213> 四孢链孢菌
<400> 94
Met Val Arg Leu Gly Leu Ala Thr Thr Leu Leu Ala Ala Ala Ser Phe
1 5 10 15
Ala Gln Ala Ala Gln Gln Lys Ala Pro Ala Val Val Pro Gly Ala Tyr
20 25 30
Ile Val Glu Tyr Glu Asp Ser His Asp Pro Thr Ser Ile Leu Ala Ser
35 40 45
Ile Lys Gly Asp Ala Thr Ile Arg Lys Asp Ile Arg His Glu Leu Phe
50 55 60
Lys Gly Ala Ser Phe Gln Phe Lys Asp Leu Asn Lys Ala Asp Asp Leu
65 70 75 80
Ala Ser Lys Ile Ala Ala Met Ser Gly Val Lys Ala Leu Tyr Pro Val
85 90 95
Arg Arg Tyr Ser Ile Pro Glu His Thr Val His Ser Thr Gly Ser Ala
100 105 110
Val Gln Glu Val Val Ala Lys Arg Asp Thr Gly Asn Asp Thr Phe Ser
115 120 125
Pro His Leu Met Thr Gln Val Asn Lys Phe Arg Asp Ser Gly Ile Thr
130 135 140
Gly Lys Gly Ile Lys Ile Ala Val Ile Asp Thr Gly Ile Asp Tyr Leu
145 150 155 160
His Pro Ala Leu Gly Gly Cys Phe Gly Pro Gly Cys Leu Val Ser Tyr
165 170 175
Gly Thr Asp Leu Val Gly Asp Asp Phe Asn Gly Ser Asn Thr Pro Val
180 185 190
Pro Asp Ser Asp Pro Met Asp Thr Cys Asn Gly His Gly Ser His Val
195 200 205
Ser Gly Val Ile Ser Gly Asn Thr Asn Asn Pro Tyr Gly Ile Ile Gly
210 215 220
Ala Ala Thr Asp Val Thr Leu Gly Ala Tyr Arg Val Phe Gly Cys Ser
225 230 235 240
Gly Asp Val Gly Asn Asp Ile Leu Ile Glu Ala Tyr Leu Lys Ala Tyr
245 250 255
Asp Asp Gly Ser Asp Ile Ile Thr Ala Ser Ile Gly Gly Ala Ser Gly
260 265 270
Trp Pro Glu Asp Ser Trp Ala Ala Val Val Ser Arg Ile Val Glu Lys
275 280 285
Gly Val Pro Cys Leu Val Ser Ala Gly Asn Asp Gly Ala Thr Gly Ile
290 295 300
Phe Tyr Ala Ser Thr Ala Ala Asn Gly Lys Arg Val Thr Ala Val Ala
305 310 315 320
Ser Val Asp Asn Ile Leu Ala Pro Ala Leu Leu Ser Glu Ala Ser Tyr
325 330 335
Ser Val Ala Asn Gly Ser Leu Ser Thr Phe Gly Phe Thr Ala Gly Ser
340 345 350
Pro Ser Ala Trp Ala Asn Val Ser Leu Pro Val Trp Ser Val Asn Phe
355 360 365
Asn Thr Ala Asp Thr Ala Asn Gly Cys Glu Ala Phe Pro Asp Asp Thr
370 375 380
Pro Asp Leu Ser Lys Tyr Ile Val Leu Ile Arg Arg Gly Thr Cys Thr
385 390 395 400
Phe Val Gln Lys Ala Gln Asn Ala Ala Ala Lys Gly Ala Lys Tyr Ile
405 410 415
Ile Tyr Tyr Asn Asn Val Ser Gly Ser Thr Lys Val Asp Val Ser Ala
420 425 430
Val Ala Gly Val Lys Ala Ala Ala Met Val Thr Ser Glu Thr Gly Val
435 440 445
Ala Trp Ile Lys Ala Leu Gln Ala Gly Thr Gln Val Thr Val Asn Met
450 455 460
Ala Asp Pro Glu Thr Ala Pro Lys Asn Leu Asn Asn Phe Pro Asn Thr
465 470 475 480
Ala Thr Pro Gly Phe Leu Ser Thr Tyr Thr Ser Trp Gly Pro Thr Tyr
485 490 495
Glu Val Asp Val Lys Pro Gln Ile Ser Ser Pro Gly Gly Met Ile Leu
500 505 510
Ser Thr Tyr Pro Arg Ala Leu Gly Ser Tyr Ala Val Leu Ser Gly Thr
515 520 525
Ser Met Ala Cys Pro Leu Ala Ala Ala Thr Trp Ala Leu Val Met Gln
530 535 540
Lys Arg Gly Thr Lys Asp Pro Lys Val Leu Glu Asn Leu Phe Ser Ala
545 550 555 560
Thr Ala His Pro Asn Leu Phe Asn Asp Gly Thr Lys Thr Tyr Pro Met
565 570 575
Leu Ala Pro Val Ala Gln Gln Gly Ala Gly Leu Ile Gln Ala Trp Asp
580 585 590
Ala Ala Asn Ala Asn Ala Leu Leu Ser Val Ser Ser Ile Ser Phe Asn
595 600 605
Asp Thr Glu His Phe Lys Pro Leu Gln Ser Phe Glu Val Thr Asn Thr
610 615 620
Gly Lys Lys Ala Val Thr Tyr Gln Leu Gly His Thr Ser Ala Ala Thr
625 630 635 640
Ala Tyr Thr Phe Ala Asn Asp Asn Ser Ile Gly Pro Ala Ala Phe Pro
645 650 655
Asn Glu Leu Val Asp Ala Lys Ala Thr Leu Val Leu Thr Pro Ala Lys
660 665 670
Leu Thr Leu Asn Pro Gly Gln Lys Lys Thr Val Thr Val Leu Ala Ile
675 680 685
Pro Pro Leu Gly Leu Asp Ala Lys Arg Leu Pro Val Tyr Ser Gly Tyr
690 695 700
Ile Thr Leu Asn Gly Thr Asp Ser Thr Gly Tyr Ser Leu Pro Tyr Gln
705 710 715 720
Gly Val Val Gly Ser Met Arg Ser Val Thr Val Leu Asp Lys Gln Asn
725 730 735
Ser Tyr Leu Ser Gln Ser Ser Asp Ala Thr Tyr Ala Pro Val Ala Ala
740 745 750
Gly Thr Thr Phe Thr Leu Pro Pro Ala Gly Lys Ala Asn Asp Thr Leu
755 760 765
Tyr Ala Asn Thr Val Tyr Pro Thr Ile Val Leu Thr Leu Ser Met Gly
770 775 780
Ser Ala Glu Val His Ala Asp Val Val Asn Ser Lys Gly Lys Thr Ile
785 790 795 800
Gly Gln Val Leu Thr Phe Pro Ala Arg Trp Asn Pro Arg Gly Thr Phe
805 810 815
Glu Trp Asn Trp Asp Gly Ala Leu Ser Asp Gly Thr Tyr Ala Pro Ala
820 825 830
Asp Thr Tyr Lys Ile Thr Leu Lys Ala Leu Lys Ile Tyr Gly Asn Ser
835 840 845
Lys Trp Pro Leu Asp Trp Glu Thr Gln Thr Thr Glu Pro Phe Thr Ile
850 855 860
Lys Tyr Ala Ala Lys Ser Lys Arg Ala Phe Thr Ala
865 870 875
<210> 95
<211> 876
<212> PRT
<213> 粗糙链孢霉
<400> 95
Met Val Arg Leu Gly Leu Ala Thr Thr Leu Leu Ala Ala Ala Ser Phe
1 5 10 15
Ala Gln Ala Ala His Gln Lys Ala Pro Ala Val Val Pro Gly Ala Tyr
20 25 30
Ile Val Glu Tyr Glu Asp Ser His Asp Pro Thr Ser Ile Leu Ala Ser
35 40 45
Ile Lys Gly Asp Ala Thr Ile Arg Lys Asp Ile Arg His Glu Leu Phe
50 55 60
Lys Gly Ala Ser Phe Gln Phe Lys Asp Leu Asn Lys Ala Asp Asp Leu
65 70 75 80
Ala Ser Lys Val Ala Ala Met Ser Gly Val Lys Ala Leu Tyr Pro Val
85 90 95
Arg Arg Tyr Ser Ile Pro Glu His Thr Val His Ser Thr Gly Ser Ala
100 105 110
Val Gln Glu Val Val Ala Lys Arg Asp Thr Gly Asn Asp Thr Phe Ser
115 120 125
Pro His Leu Met Thr Gln Val Asn Lys Phe Arg Asp Ser Gly Ile Thr
130 135 140
Gly Lys Gly Ile Lys Ile Ala Val Ile Asp Thr Gly Val Asp Tyr Leu
145 150 155 160
His Pro Ala Leu Gly Gly Cys Phe Gly Pro Gly Cys Leu Val Ser Tyr
165 170 175
Gly Thr Asp Leu Val Gly Asp Asp Phe Asn Gly Ser Asn Thr Pro Val
180 185 190
Pro Asp Ser Asp Pro Met Asp Thr Cys Asn Gly His Gly Ser His Val
195 200 205
Leu Gly Leu Leu Ser Ala Asn Thr Asn Asn Pro Tyr Gly Ile Ile Gly
210 215 220
Ala Ala Pro Asp Val Thr Leu Gly Ala Tyr Arg Val Phe Gly Cys Ser
225 230 235 240
Gly Asp Val Gly Asn Asp Ile Leu Ile Glu Ala Tyr Leu Lys Ala Tyr
245 250 255
Asp Asp Gly Ser Asp Ile Ile Thr Ala Ser Ile Gly Gly Ala Ser Gly
260 265 270
Trp Pro Glu Asp Ser Trp Ala Ala Val Val Ser Arg Ile Val Glu Lys
275 280 285
Gly Val Pro Cys Leu Val Ser Ala Gly Asn Asp Gly Ala Thr Gly Ile
290 295 300
Phe Tyr Ala Ser Thr Ala Ala Asn Gly Lys Arg Val Thr Ala Val Ala
305 310 315 320
Ser Val Asp Asn Ile Leu Ala Pro Ala Leu Leu Ser Glu Ala Ser Tyr
325 330 335
Ser Val Ala Asn Gly Ser Leu Ser Thr Phe Gly Phe Thr Ala Gly Ser
340 345 350
Pro Ser Ala Trp Ala Asn Val Ser Leu Pro Val Trp Ser Val Asn Phe
355 360 365
Asn Thr Ala Asp Ala Ala Asn Gly Cys Glu Ala Phe Pro Asp Asp Thr
370 375 380
Pro Asp Leu Ser Lys Tyr Ile Val Leu Ile Arg Arg Gly Thr Cys Thr
385 390 395 400
Phe Val Gln Lys Ala Gln Asn Ala Ala Ala Lys Gly Ala Lys Tyr Ile
405 410 415
Ile Tyr Tyr Asn Asn Ala Ser Gly Ser Thr Lys Val Asp Val Ser Ala
420 425 430
Val Ala Asp Val Lys Ala Ala Ala Met Val Thr Ser Glu Thr Gly Ala
435 440 445
Ala Trp Ile Lys Ala Leu Gln Ala Gly Thr Gln Val Thr Val Asn Met
450 455 460
Ala Asp Pro Glu Thr Ala Pro Lys Asn Leu Asn Asn Phe Pro Asn Thr
465 470 475 480
Ala Thr Pro Gly Phe Leu Ser Thr Tyr Thr Ser Trp Gly Pro Thr Tyr
485 490 495
Glu Val Asp Val Lys Pro Gln Ile Ser Ser Pro Gly Gly Met Ile Leu
500 505 510
Ser Thr Tyr Pro Arg Ala Leu Gly Ser Tyr Ala Val Leu Ser Gly Thr
515 520 525
Ser Met Ala Cys Pro Leu Ala Ala Ala Thr Trp Ala Leu Val Met Gln
530 535 540
Lys Arg Gly Thr Lys Asp Pro Lys Val Leu Glu Asn Leu Phe Ser Ala
545 550 555 560
Thr Ala His Pro Asn Leu Phe Asn Asp Gly Thr Lys Thr Tyr Pro Met
565 570 575
Leu Ala Pro Val Ala Gln Gln Gly Ala Gly Leu Ile Gln Ala Trp Asp
580 585 590
Ala Ala Asn Ala Asn Ala Leu Leu Ser Val Ser Ser Ile Ser Phe Asn
595 600 605
Asp Thr Glu His Phe Lys Pro Leu Gln Ser Phe Glu Val Thr Asn Thr
610 615 620
Gly Lys Lys Ala Val Thr Tyr Gln Leu Gly His Thr Ser Ala Ala Thr
625 630 635 640
Ala Tyr Thr Phe Ala Asn Asp Thr Ser Ile Gly Pro Ala Ala Phe Pro
645 650 655
Asn Glu Leu Val Asp Ala Lys Ala Thr Leu Val Leu Thr Pro Ala Lys
660 665 670
Leu Thr Leu Asn Pro Gly Gln Lys Lys Thr Val Thr Val Leu Ala Ile
675 680 685
Pro Pro Leu Gly Leu Asp Ala Lys Arg Leu Pro Val Tyr Ser Gly Tyr
690 695 700
Ile Thr Leu Asn Gly Thr Asp Ser Thr Gly Tyr Ser Leu Pro Tyr Gln
705 710 715 720
Gly Val Val Gly Ser Met Arg Ser Val Thr Val Leu Asp Lys Gln Asn
725 730 735
Ser Tyr Leu Ser Gln Ser Ser Asp Ala Thr Tyr Ala Pro Val Ala Ala
740 745 750
Gly Thr Thr Phe Thr Leu Pro Pro Ala Gly Lys Ala Asn Asp Thr Leu
755 760 765
Tyr Ala Thr Thr Val Tyr Pro Thr Ile Val Leu Thr Leu Ser Met Gly
770 775 780
Ser Ala Glu Val His Ala Asp Val Val Asn Ser Lys Gly Lys Thr Ile
785 790 795 800
Gly Gln Val Leu Thr Phe Pro Ala Arg Trp Asn Pro Arg Gly Thr Phe
805 810 815
Glu Trp Asn Trp Asp Gly Ala Leu Ser Asp Gly Thr Tyr Ala Pro Ala
820 825 830
Asp Thr Tyr Lys Ile Thr Leu Lys Ala Leu Lys Ile Tyr Gly Asn Ser
835 840 845
Lys Trp Pro Leu Asp Trp Glu Thr Gln Thr Thr Glu Pro Phe Thr Ile
850 855 860
Lys Tyr Ala Ala Lys Ser Lys Arg Ala Phe Thr Ala
865 870 875
<210> 96
<211> 902
<212> PRT
<213> 稻瘟病菌
<400> 96
Met Val Gly Lys Ala Ile Val Leu Ser Leu Phe Ala Ala Thr Cys Leu
1 5 10 15
Ala Ser Gln Ala Thr Lys Pro Val Glu Glu Lys Ala Pro Ile Ile Pro
20 25 30
Gly Leu Tyr Ile Val Glu Tyr Glu Glu Gly Gln Met Asp Thr Ala Thr
35 40 45
Leu Ser Asn Glu Ile Ala Ser Asp Ala His Val Lys Met Asn Leu Asp
50 55 60
Phe Glu Leu Phe Lys Gly Ala Ser Ile Gln Phe His Asp Ser Ala His
65 70 75 80
Val Glu Glu Thr Ala Ala Lys Ile Ala Glu Met Pro Ala Ile Lys Arg
85 90 95
Met Trp Pro Gln Arg Leu Tyr Arg Ile Pro Asp Tyr Gln Val Ser Trp
100 105 110
Ala Gly Asp Met Pro Asp Ala Gln Ala Lys Ala Val Met Arg Arg Gln
115 120 125
Ser Asp Gly Asn Asp Thr Phe Thr Pro His Leu Met Thr Gln Val Asn
130 135 140
Gln Leu Arg Asn Lys Gly Val Val Gly Lys Gly Ile Lys Val Ala Val
145 150 155 160
Ile Asp Ser Gly Val Asp Trp Lys His Pro Ala Leu Gly Gly Cys Phe
165 170 175
Gly Arg Pro Glu Cys Leu Val Ser Phe Gly Tyr Asp Leu Val Gly Asp
180 185 190
Ala Tyr Asn Gly Thr Asn Thr Ala Val Pro Asp Asn Asp Pro Met Asp
195 200 205
Cys Gly Gly His Gly Thr His Val Ser Gly Ile Ile Ala Ala Gln Thr
210 215 220
Asn Asn Pro Phe Gly Ile Ile Gly Ala Ala Thr Gly Val Thr Met Gly
225 230 235 240
His Tyr Arg Val Phe Gly Cys Glu Gly Ser Ala Ser Asp Asp Val Leu
245 250 255
Ile Gln Ala Tyr Asn Met Ala Tyr Gln Asp Gly Ala Asp Leu Ile Thr
260 265 270
Ala Ser Ile Ala Gly Ser Ser Gly Trp Pro Glu Gln Ala Trp Ala Val
275 280 285
Ala Val Thr Arg Ile Val Asp Lys Gly Val Pro Cys Val Ile Ser Ala
290 295 300
Gly Asn Asp Gly Ala Thr Gly Met Phe Phe Thr Ser Ser Ala Ala Asp
305 310 315 320
Ala Arg Gly Ala Thr Ala Ile Ala Ser Phe Asp Asn Ser Val Thr Pro
325 330 335
Gln Leu Leu Ser Asn Ala Thr Tyr Arg Val Gly Gly Gly Gln Pro Val
340 345 350
Ser Phe Ser Tyr Ser Ala Gly Ser Pro Ala Lys Trp Gly Asn Val Thr
355 360 365
Lys Gln Leu Trp Ala Val Asn Phe Asn Thr Asn Asp Thr Gln Asn Ala
370 375 380
Cys Asp Pro Leu Pro Ala Asn Thr Pro Asp Leu Ser Asn Phe Ile Val
385 390 395 400
Leu Ile Arg Arg Gly Thr Cys Pro Phe Thr Gln Lys Ala Thr Asn Val
405 410 415
Arg Ala Lys Gly Ala Lys Tyr Val Phe Phe Tyr Asn Asn Val Pro Gly
420 425 430
Thr Leu Ser Ile Ser Val Thr Ala Val Asp Gly Ile Leu Gly Ala Ala
435 440 445
Thr Val Ser Ala Glu Ile Gly Lys Ser Trp Ile Ala Glu Leu Ala Ala
450 455 460
Gly Arg Asn Val Thr Leu Glu Met Thr Asp Pro Leu Thr Ala Pro Lys
465 470 475 480
Phe Leu Leu Ser Ala Pro Asn Ser Ala Thr Gly Gly Ala Pro Ser Thr
485 490 495
Tyr Thr Ser Trp Gly Pro Thr Phe Glu Gly Gln Val Lys Pro Gln Val
500 505 510
Ala Ala Pro Gly Gly Asn Ile Leu Ser Thr Tyr Pro Leu Ala Lys Gly
515 520 525
Gly Tyr Ala Val Leu Ser Gly Thr Ser Met Ala Cys Pro Leu Thr Ala
530 535 540
Gly Val Tyr Ala Leu Ile Met Ser Val Arg Gly Ile Lys Asp Pro Lys
545 550 555 560
Thr Leu Glu Asn Leu Val Ala Ser Thr Ala Lys Pro Thr Leu Phe Asn
565 570 575
Ser Gly Ser Thr Ser Ala Ser Tyr Leu Ala Pro Val Pro Gln Gln Gly
580 585 590
Ala Gly Leu Ile Gln Ala Tyr Asp Ala Ala Tyr Thr Thr Thr Leu Leu
595 600 605
Ser Val Ser Ser Leu Ala Phe Asn Asp Thr Asp Asn Phe Ala Pro Val
610 615 620
Gln Asn Phe Thr Ile Ser Asn Lys Gly Ser Ser Ser Val Thr Tyr Thr
625 630 635 640
Leu Ser Gln Arg Gly Ala Ala Thr Ala Phe Thr Phe Ser Ser Asn Ala
645 650 655
Ser Asn Ile Thr Pro Glu Val Phe Pro Asn Glu Leu Val Ala Asn Tyr
660 665 670
Ala Asn Leu Lys Phe Ser Glu Glu Lys Leu Glu Val Gly Pro Gly Gly
675 680 685
Asn Ala Gln Ile Ala Val Thr Val Thr Pro Pro Ser Gly Leu Asp Ala
690 695 700
Arg Arg Leu Pro Val Tyr Ser Gly Tyr Val Thr Ile Asn Gly Thr Asp
705 710 715 720
Gly Ser Ser Phe Ser Leu Pro Tyr Met Gly Ile Ala Gly Ser Ile His
725 730 735
Ala Gln Arg Thr Leu Asp Pro Gln Gln Thr Phe Leu Ser Ser Asn Gln
740 745 750
Ile Pro Arg Asn Phe Thr Glu Asp Asn Pro Pro Arg Val Pro Ala Gly
755 760 765
His Gln Phe Asp Leu Pro Pro Gln Gly Gln Ala Asn Asn Thr Gln Tyr
770 775 780
Val Leu Val Ser Glu Met Pro Arg Phe Ser Ile Asn Leu Ala Trp Gly
785 790 795 800
Thr Pro Leu Leu Arg Ala Asp Val Val Ala Leu Gly Gly Asn Ser Thr
805 810 815
Ala Ala Glu Gln Pro Leu Ala Glu Thr Glu Val Leu Gly Leu Lys Thr
820 825 830
Val Gly Ser Leu Gln Tyr Phe Pro Gln Arg Tyr Gln Ala Arg Thr Arg
835 840 845
Ser Phe Val Arg Trp Asp Gly Ile Leu Asp Ser Gly Lys Tyr Ala Pro
850 855 860
Ser Gly His Tyr Lys Ile Val Phe Arg Ala Leu Arg Val Phe Gly Asn
865 870 875 880
Ser Glu Lys Gln Asp Glu Tyr Asp Val Val Glu Thr Val Pro Phe Lys
885 890 895
Ile Arg Tyr Ile Asn Lys
900
<210> 97
<211> 1006
<212> PRT
<213> 球毛壳霉
<400> 97
Met Ala Leu Phe Ser Ala Val Glu Ala Trp His Pro Gly Leu Ser Pro
1 5 10 15
Gly Ala Thr Pro Ser Val Asp Gly Asp His Pro Arg Gly Gly Ala Val
20 25 30
Arg Gly Ala Leu Ser Gln Glu Gly Asp Ile Ser Leu Gln Glu His Ala
35 40 45
Pro His Glu Ser Cys Tyr Leu Ile Tyr Tyr Val Gln Asn Ala Ser Asp
50 55 60
His Thr Val Pro Pro Gln Ala Ala Pro Arg Glu Glu Ala Arg Pro Met
65 70 75 80
Tyr Gly Gln Val Lys Asp Glu Leu Lys His Ser Lys Leu Ser His Asp
85 90 95
Ile Arg Leu Gln Leu Phe Pro Leu Leu Trp Ala Ser Phe Leu Ser Thr
100 105 110
Ser Lys Gly Ile Leu Leu Gln Arg Glu Leu Arg Asn Gly Thr Cys Gln
115 120 125
Ala Gln Pro Asn Ser Asp His Pro Ser Ala Gly Gly Ser Ala Arg Pro
130 135 140
Leu Cys Ala Pro Pro Ser Val Asp Ser Asn Ala Phe Val Asn Asn Leu
145 150 155 160
Gly Gly Lys Ala Ser Leu Arg Lys Asp Leu Arg Phe Lys Leu Phe Lys
165 170 175
Gly Ala Ser Ile Gln Phe Lys Asp Thr Lys Asn Ala Asp Gln Met Ile
180 185 190
Ala Lys Val Ala Thr Met Pro Lys Ile Lys Ala Val Tyr Pro Val Arg
195 200 205
Arg Tyr Pro Val Pro Arg His Val Val His Ser Thr Gly Asp Ala Val
210 215 220
Glu Ala Val Leu Ser Lys Arg Gln Glu Gly Ser Asp Thr Phe Ser Thr
225 230 235 240
His Leu Met Thr Gln Val Asn Arg Phe Lys Asp Lys Ala Val Thr Gly
245 250 255
Lys Gly Ile Lys Ile Gly Ile Ile Asp Thr Gly Thr Asp Tyr Leu His
260 265 270
Pro Ala Leu Gly Gly Cys Phe Gly Glu Gly Cys Leu Val Ser Tyr Gly
275 280 285
Thr Asp Leu Val Gly Asp Lys Phe Asn Gly Ala Asn Thr Pro Val Pro
290 295 300
Asp Ala Asp Pro Leu Asp Glu Cys Asn Gly His Gly Thr His Val Ala
305 310 315 320
Gly Ile Ile Ala Ala Gln Ser Asn Asn Pro Tyr Gly Ile Val Gly Ala
325 330 335
Ala Glu Gly Val Thr Leu Gly Ser Tyr Arg Val Phe Gly Cys Thr Gly
340 345 350
Asp Val Gly Asn Asp Ile Leu Ile Ala Ala Tyr Asn Met Ala Tyr Glu
355 360 365
Asp Gly Ser Asp Ile Ile Thr Ala Ser Ile Gly Gly Ala Ser Gly Trp
370 375 380
Ser Glu Asp Pro Trp Ala Ala Val Val Ser Arg Ile Val Asp Asn Gly
385 390 395 400
Val Pro Cys Val Val Ser Ala Gly Asn Asp Gly Ala Ala Gly Ile Phe
405 410 415
Tyr Ala Ser Thr Ala Ala Asn Gly Lys Arg Val Thr Ala Ile Ala Ser
420 425 430
Val Asp Asn Thr Val Thr Pro Ala Leu Leu Ser Asn Ala Ser Phe Ser
435 440 445
Ile Asn Gly Thr Ser Asp Phe Phe Gly Phe Thr Ala Gly Glu Pro Gly
450 455 460
Thr Trp Asp Asp Val Thr Leu Pro Leu Trp Ser Val Gly Phe Asp Thr
465 470 475 480
Thr Asp Pro Ala Asn Gly Cys Glu Pro Tyr Pro Asp Ser Thr Pro Asp
485 490 495
Leu Ser Gly Tyr Ile Val Leu Val Arg Arg Gly Thr Cys Ala Phe Val
500 505 510
Asp Lys Ala Ala Asn Ala Val Ala Lys Gly Ala Lys Tyr Val Met Phe
515 520 525
Tyr Asn Asn Val Asp Gly Thr Val Ser Val Ser Ala Asn Val Glu Gly
530 535 540
Val Val Gly Val Ala Met Val Thr Pro Glu Gln Gly Glu Ile Trp Val
545 550 555 560
Arg Ala Leu Glu Ala Gly Ser Glu Val Val Val His Met Thr Asp Pro
565 570 575
Leu Lys Ala Gly Thr Phe Leu Ser Ser Ser Lys Asn Thr Leu Ser Gly
580 585 590
Gly Phe Leu Ser Asp Phe Thr Ser Trp Gly Pro Thr Tyr Glu Val Glu
595 600 605
Val Lys Pro Gln Phe Ser Thr Pro Gly Gly Leu Ile Leu Ser Thr Tyr
610 615 620
Pro Arg Ala Leu Gly Ser Tyr Gly Val Leu Ser Gly Thr Ser Met Ala
625 630 635 640
Cys Pro Met Ala Ala Ala Ile Tyr Ala Leu Leu Met Thr Ala Arg Asn
645 650 655
Thr Lys Asp Pro Lys Thr Leu Glu Asn Val Leu Ser Ala Thr Ala Arg
660 665 670
Pro Asn Val Phe Arg Leu Gln Gly Lys Ser Leu Pro Val Leu Ala Pro
675 680 685
Val Ala Gln Gln Gly Ala Gly Leu Leu Gln Ala Trp Asp Ala Ala Arg
690 695 700
Ala Thr Thr Leu Leu Ser Val Ser Ser Ile Ser Phe Asn Asp Thr Asp
705 710 715 720
Asn Phe Ser Pro Val Gln Asn Phe Ser Ile Ser Asn Thr Gly Ala Thr
725 730 735
Ala Val Thr Tyr Ser Leu Ser Asn Ile Gly Ala Ala Thr Ala Tyr Thr
740 745 750
Phe Asn Asp Glu Lys Thr Ile Ser Pro Ala Ala Phe Pro Asn Glu Leu
755 760 765
Thr Gly Asp Phe Ala Ser Leu Ser Phe Ser Pro Ser Asn Phe Thr Ile
770 775 780
Pro Ala Gly Gln Arg Lys Ile Ile Thr Val Thr Ala Thr Pro Pro Lys
785 790 795 800
Gly Val Asn Val Lys Arg Leu Pro Val Tyr Ser Gly Tyr Ile Ala Ile
805 810 815
Asn Gly Ser Asp Ser Ser Ala Leu Ser Leu Pro Tyr Leu Gly Val Ala
820 825 830
Gly Ser Met Arg Ser Val Val Val Leu Asp Ser Lys Ala Ala Leu Ile
835 840 845
Ser Ser Ala Arg Asp Gln Ser Asn Thr Pro Val Pro Ala Gly Val Thr
850 855 860
Phe Val Leu Pro Pro Pro Gly Phe Ser Asn Asp Ser Ser Tyr Ala Asn
865 870 875 880
Arg Thr Glu Arg Pro Lys Leu Val Val Ser Leu Ala Met Gly Ser Ala
885 890 895
Val Leu Arg Ala Asp Ile Val Pro Val Ser Asn Cys Thr Thr Val Ala
900 905 910
Arg Thr Ala Gly Val Val Phe Gly Thr Lys Thr Ile Gly Gln Pro Glu
915 920 925
Gly Leu Pro Ala Leu Trp Asn Pro Arg Gly Arg Phe Glu Tyr Ser Trp
930 935 940
Asp Gly Lys Leu Asp Asp Gly Thr Tyr Ala Pro Ala Gly Arg Tyr Lys
945 950 955 960
Phe Ala Val Lys Ala Leu Arg Ile Phe Gly Asp Arg Glu Glu Val Ala
965 970 975
Asp Tyr Asp Ala Ala Glu Thr Thr Asp Phe Arg Ile Arg Tyr Leu Pro
980 985 990
Ala Pro Ser Ala Lys Phe Arg Arg Gln Glu Gln Gly Cys Glu
995 1000 1005
<210> 98
<211> 541
<212> PRT
<213> 里氏木霉
<400> 98
Met Arg Ser Val Val Ala Leu Ser Met Ala Ala Val Ala Gln Ala Ser
1 5 10 15
Thr Phe Gln Ile Gly Thr Ile His Glu Lys Ser Ala Pro Val Leu Ser
20 25 30
Asn Val Glu Ala Asn Ala Ile Pro Asp Ala Tyr Ile Ile Lys Phe Lys
35 40 45
Asp His Val Gly Glu Asp Asp Ala Ser Lys His His Asp Trp Ile Gln
50 55 60
Ser Ile His Thr Asn Val Glu Gln Glu Arg Leu Glu Leu Arg Lys Arg
65 70 75 80
Ser Asn Val Phe Gly Ala Asp Asp Val Phe Asp Gly Leu Lys His Thr
85 90 95
Phe Lys Ile Gly Asp Gly Phe Lys Gly Tyr Ala Gly His Phe His Glu
100 105 110
Ser Val Ile Glu Gln Val Arg Asn His Pro Asp Val Glu Tyr Ile Glu
115 120 125
Arg Asp Ser Ile Val His Thr Met Leu Pro Leu Glu Ser Lys Asp Ser
130 135 140
Ile Ile Val Glu Asp Ser Cys Asn Gly Glu Thr Glu Lys Gln Ala Pro
145 150 155 160
Trp Gly Leu Ala Arg Ile Ser His Arg Glu Thr Leu Asn Phe Gly Ser
165 170 175
Phe Asn Lys Tyr Leu Tyr Thr Ala Asp Gly Gly Glu Gly Val Asp Ala
180 185 190
Tyr Val Ile Asp Thr Gly Thr Asn Ile Glu His Val Asp Phe Glu Gly
195 200 205
Arg Ala Lys Trp Gly Lys Thr Ile Pro Ala Gly Asp Glu Asp Glu Asp
210 215 220
Gly Asn Gly His Gly Thr His Cys Ser Gly Thr Val Ala Gly Lys Lys
225 230 235 240
Tyr Gly Val Ala Lys Lys Ala His Val Tyr Ala Val Lys Val Leu Arg
245 250 255
Ser Asn Gly Ser Gly Thr Met Ser Asp Val Val Lys Gly Val Glu Tyr
260 265 270
Ala Ala Leu Ser His Ile Glu Gln Val Lys Lys Ala Lys Lys Gly Lys
275 280 285
Arg Lys Gly Phe Lys Gly Ser Val Ala Asn Met Ser Leu Gly Gly Gly
290 295 300
Lys Thr Gln Ala Leu Asp Ala Ala Val Asn Ala Ala Val Arg Ala Gly
305 310 315 320
Val His Phe Ala Val Ala Ala Gly Asn Asp Asn Ala Asp Ala Cys Asn
325 330 335
Tyr Ser Pro Ala Ala Ala Thr Glu Pro Leu Thr Val Gly Ala Ser Ala
340 345 350
Leu Asp Asp Ser Arg Ala Tyr Phe Ser Asn Tyr Gly Lys Cys Thr Asp
355 360 365
Ile Phe Ala Pro Gly Leu Ser Ile Gln Ser Thr Trp Ile Gly Ser Lys
370 375 380
Tyr Ala Val Asn Thr Ile Ser Gly Thr Ser Met Ala Ser Pro His Ile
385 390 395 400
Cys Gly Leu Leu Ala Tyr Tyr Leu Ser Leu Gln Pro Ala Gly Asp Ser
405 410 415
Glu Phe Ala Val Ala Pro Ile Thr Pro Lys Lys Leu Lys Glu Ser Val
420 425 430
Ile Ser Val Ala Thr Lys Asn Ala Leu Ser Asp Leu Pro Asp Ser Asp
435 440 445
Thr Pro Asn Leu Leu Ala Trp Asn Gly Gly Gly Cys Ser Asn Phe Ser
450 455 460
Gln Ile Val Glu Ala Gly Ser Tyr Thr Val Lys Pro Lys Gln Asn Lys
465 470 475 480
Gln Ala Lys Leu Pro Ser Thr Ile Glu Glu Leu Glu Glu Ala Ile Glu
485 490 495
Gly Asp Phe Glu Val Val Ser Gly Glu Ile Val Lys Gly Ala Lys Ser
500 505 510
Phe Gly Ser Lys Ala Glu Lys Phe Ala Lys Lys Ile His Asp Leu Val
515 520 525
Glu Glu Glu Ile Glu Glu Phe Ile Ser Glu Leu Ser Glu
530 535 540
<210> 99
<211> 537
<212> PRT
<213> 深绿木霉
<400> 99
Met Arg Ser Val Ile Ala Leu Ser Val Ala Ala Val Ala Gln Ala Ser
1 5 10 15
Ser Phe Gln Val Gly Thr Ile His Glu Ser Ser Ala Pro Val Leu Ser
20 25 30
Asn Ile Glu Ala Asn Ala Ile Pro Asp Asn Tyr Ile Ile Lys Phe Lys
35 40 45
Asp His Val Asp Glu Ala Gly Ala Asp Lys His Gln Asn Trp Ile Gln
50 55 60
Ser Ile His Asp Glu Gly Glu Gln Gln Arg Leu Glu Leu Arg Lys Arg
65 70 75 80
Ser Ser Ile Phe Gly Ala Asp Glu Ala Phe Asp Gly Leu Lys His Thr
85 90 95
Phe Lys Ile Gly Asp Phe Lys Gly Tyr Ala Gly His Phe Thr Glu Asp
100 105 110
Ile Ile Glu Gln Val Arg Asn His Pro Asp Val Glu Tyr Ile Glu Arg
115 120 125
Asp Thr Ile Val His Thr Met Leu Pro Leu Asp Ser Gln Asp Ser Val
130 135 140
Val Val Glu Asp Ser Cys Asn Pro Glu Thr Glu Lys Gln Ala Pro Trp
145 150 155 160
Gly Leu Ala Arg Ile Ser His Arg Asp Thr Leu Gly Phe Gly Thr Phe
165 170 175
Asn Lys Tyr Leu Tyr Thr Ala Asp Gly Gly Glu Gly Val Asp Ala Tyr
180 185 190
Val Ile Asp Thr Gly Thr Asn Ile Glu His Val Asp Phe Glu Gly Arg
195 200 205
Ala Lys Trp Gly Lys Thr Ile Pro Ala Gly Asp Glu Asp Glu Asp Gly
210 215 220
Asn Gly His Gly Thr His Cys Ser Gly Thr Val Ala Gly Lys Lys Tyr
225 230 235 240
Gly Val Ala Lys Lys Ala His Val Tyr Ala Val Lys Val Leu Arg Ser
245 250 255
Asn Gly Ser Gly Thr Met Ser Asp Val Val Lys Gly Val Glu Tyr Ala
260 265 270
Ala Leu Ser His Gln Glu Gln Val Lys Gln Ala Lys Ala Gly Lys Arg
275 280 285
Lys Gly Phe Lys Gly Ser Val Ala Asn Met Ser Leu Gly Gly Gly Lys
290 295 300
Thr Ser Ala Leu Asp Ala Ala Val Asn Ala Ala Val Lys Ala Gly Val
305 310 315 320
His Phe Ala Val Ala Ala Gly Asn Asp Asn Ala Asp Ala Cys Asn Tyr
325 330 335
Ser Pro Ala Ala Ala Thr Glu Pro Val Thr Val Gly Ala Ser Ala Leu
340 345 350
Asp Asp Ser Arg Ala Tyr Phe Ser Asn Tyr Gly Lys Cys Thr Asp Ile
355 360 365
Phe Ala Pro Gly Leu Ser Ile Gln Ser Thr Trp Ile Gly Ser Lys Tyr
370 375 380
Ala Val Asn Thr Ile Ser Gly Thr Ser Met Ala Ser Pro His Ile Ala
385 390 395 400
Gly Leu Leu Ala Tyr Tyr Leu Ser Leu Gln Pro Ala Gly Asp Ser Glu
405 410 415
Phe Ala Val Ala Pro Ile Thr Pro Asn Lys Leu Lys Lys Asp Leu Ile
420 425 430
Thr Ile Ala Thr Gln Gly Thr Leu Ser Asp Ile Pro Ala Asp Thr Pro
435 440 445
Asn Leu Leu Ala Trp Asn Gly Ala Gly Cys Ser Asn Tyr Ser Gln Ile
450 455 460
Val Lys Ala Gly Gly Tyr Lys Ala Lys Ala Gln Lys Gln Thr Lys Leu
465 470 475 480
Pro Ala Thr Ile Glu Glu Leu Glu Asp Ala Ile Glu Gly Asp Phe Glu
485 490 495
Val Val Ser Gly Lys Ile Val Lys Gly Ala Lys Ser Phe Gly Ser Lys
500 505 510
Ala Glu Lys Phe Ala Lys Lys Ile His Asp Leu Val Glu Glu Glu Ile
515 520 525
Asp Glu Phe Met Ser Glu Leu Ser Glu
530 535
<210> 100
<211> 1670
<212> PRT
<213> 绿木霉
<400> 100
Met Arg Ser Val Val Ala Leu Ser Val Ala Ala Val Ala Gln Ala Ser
1 5 10 15
Ser Phe Ser Ile Gly Thr Ile His Glu Ser Ser Ala Pro Val Leu Ser
20 25 30
Thr Val Glu Ala Asn Ala Ile Pro Asp Ala Tyr Ile Ile Lys Phe Lys
35 40 45
Asp His Val Gly Glu Ala Asp Ala Asp Lys His His Asn Trp Leu Gln
50 55 60
Asn Ile His Ser Ser Gly Glu Gln Glu Arg Leu Glu Leu Arg Lys Arg
65 70 75 80
Ser Asn Ile Phe Gly Ser Asp Asp Ala Phe Ala Gly Leu Lys His Thr
85 90 95
Phe Lys Met Asp Gly Phe Lys Gly Tyr Ala Gly His Phe His Glu Ser
100 105 110
Val Ile Glu Gln Val Arg Asn His Pro Asp Val Glu Tyr Ile Glu Arg
115 120 125
Asp Ser Ile Val His Thr Met Leu Pro Leu Glu Ser Lys Asp Thr Ile
130 135 140
Ile Val Glu Asp Ser Cys Asn Gly Glu Thr Glu Lys Gln Ala Pro Trp
145 150 155 160
Gly Leu Ala Arg Ile Ser His Arg Glu Thr Leu Asn Phe Gly Ser Phe
165 170 175
Asn Lys Tyr Leu Tyr Thr Ala Glu Gly Gly Glu Gly Val Asp Ala Tyr
180 185 190
Val Ile Asp Thr Gly Thr Asn Ile Asp His Val Asp Phe Glu Gly Arg
195 200 205
Ala Lys Trp Gly Lys Thr Ile Pro Ala Gly Asp Glu Asp Glu Asp Gly
210 215 220
Asn Gly His Gly Thr His Cys Ser Gly Thr Val Ala Gly Lys Lys Tyr
225 230 235 240
Gly Val Ala Lys Lys Ala His Val Tyr Ala Val Lys Val Leu Arg Ser
245 250 255
Asn Gly Ser Gly Thr Met Ser Asp Val Val Lys Gly Val Glu Trp Ala
260 265 270
Ala Leu Ala His Ile Glu Ala Val Lys Gln Ala Lys Ala Gly Lys Arg
275 280 285
Lys Gly Phe Lys Gly Ser Val Ala Asn Met Ser Leu Gly Gly Gly Lys
290 295 300
Thr Gln Ala Leu Asp Ala Ala Val Asn Ala Ala Val Asn Ala Gly Val
305 310 315 320
His Phe Ala Val Ala Ala Gly Asn Asp Asn Ala Asp Ala Cys Asn Tyr
325 330 335
Ser Pro Ala Ala Ala Ser Ala Pro Leu Thr Val Gly Ala Ser Ala Leu
340 345 350
Asp Asp Ser Arg Ala Tyr Phe Ser Asn Tyr Gly Lys Cys Thr Asp Ile
355 360 365
Phe Ala Pro Gly Leu Ser Ile Gln Ser Thr Trp Ile Gly Ser Lys Tyr
370 375 380
Ala Val Asn Thr Ile Ser Gly Thr Ser Met Ala Ser Pro His Ile Cys
385 390 395 400
Gly Leu Leu Ala Tyr Tyr Leu Ser Leu Gln Pro Ala Gly Asp Ser Glu
405 410 415
Phe Ala Val Ala Pro Ile Thr Pro Lys Lys Leu Lys Glu Asn Ile Val
420 425 430
Ser Ile Ala Thr Lys Gly Ala Leu Ser Asp Leu Pro Asp Ser Asp Thr
435 440 445
Pro Asn Leu Leu Ala Trp Asn Gly Gly Gly Cys Ser Asn Phe Ser Gln
450 455 460
Ile Leu Glu Ala Gly Ser Tyr Thr Val Lys Pro Lys Ala Lys His Ala
465 470 475 480
Lys Leu Pro Ala Thr Val Ser Glu Leu Glu Lys Ala Ile Glu Gly Asp
485 490 495
Phe Glu Val Val Ser Gly Lys Ile Val Lys Gly Ala Lys Thr Phe Gly
500 505 510
Ser Lys Ala Glu Lys Phe Ala Lys Lys Ile His Asp Leu Val Glu Glu
515 520 525
Glu Ile Glu Glu Phe Ile Ser Glu Leu Ser Asp Asn Gly His Asn Ala
530 535 540
Pro Met Arg Leu Ser Val Arg Gly Phe Met Asp Arg Tyr Gln Glu Arg
545 550 555 560
Leu Val Leu Phe Leu Gly Ile Arg His Ala Leu Trp Ala Arg Ser His
565 570 575
Gly Phe Ile Phe Ala Phe Ser Tyr Tyr Ser Tyr Leu Ala Ser Pro Ile
580 585 590
Trp Leu Arg Lys Thr Gln Met Ala Ala Ser Cys Gln Lys Pro Gln Ser
595 600 605
Leu Thr Arg Lys Ala Gly Ala Lys Arg Cys Lys Thr Gly Ser Phe Arg
610 615 620
Thr Ile Trp Gln Asn Gly Phe Phe Arg Tyr Gly Arg Arg His Arg Arg
625 630 635 640
Leu Leu Pro Leu Asn Trp Gln Glu His Ala Lys Arg Lys Thr Lys Ile
645 650 655
Ser Pro Ser His Tyr His Ser Thr Arg His Arg His Leu Leu His Ile
660 665 670
Val Gly Gln Gly Val Thr Ile Arg Ala Thr Ser Leu Arg Ser Leu Tyr
675 680 685
Ser Leu Asn Asn Asp Asn Ala Ser Gln Glu Gln Leu Gln Lys Pro Glu
690 695 700
Ser Ser Pro Pro Glu Gln Glu Gln Pro Tyr Ala Ala Glu Arg Asp Ala
705 710 715 720
Tyr Leu Ala Ala Thr Leu Ser Tyr Asn Arg Ala Gln Pro Gln Ser Asn
725 730 735
Gly Gly Gly Lys Ala Ser Arg Leu His Arg Gln Gln Ser Val Arg Phe
740 745 750
Ala Gly Pro Asn Ala Gln Pro Gln Arg Arg Leu Ala Ala Arg Ala Ser
755 760 765
Met Ala Leu Val Gly Asn Ile Asn Met Pro Arg Arg Pro Val Gly Ser
770 775 780
Gln His Leu Ser Gln Ser Ser Phe Glu Pro Gly Glu Asp Ala Ala Ser
785 790 795 800
Leu Ser Tyr Ala Arg Leu Arg Lys Ser Arg Ser Met Tyr Thr Pro Ser
805 810 815
Ile Pro Asn Thr Pro Thr Tyr Thr Met Asp Ser Gly Asp Ser Val Asp
820 825 830
Val Leu Asn Gly Trp Leu Pro Thr Pro Gln Ile Arg Lys Glu Asn Glu
835 840 845
Pro Leu Arg Ser Phe Gly Gly Pro Ala Leu Arg Ser Pro Lys Ser Met
850 855 860
Gly Phe Leu Ser Asn Asp Cys Glu Ile Ser Thr Ser Arg Ala Ser Ile
865 870 875 880
Ser Ser Gly His Ala Ser Val Gln Gln Thr Arg Asn Gly Phe Tyr Glu
885 890 895
Pro Ser Glu Arg Ser Ser Arg Leu Gln Ser Arg Pro Ser Ser Phe Phe
900 905 910
Arg Ser Ser Tyr Arg His Ala Pro Asn Pro Thr Ser Phe Pro Lys Ser
915 920 925
Leu Arg Gly Ser Ser Asn Asn Thr Ser Phe Leu Ser Ser Glu Ser Ile
930 935 940
Tyr Gly Ala Lys Phe Pro Pro Gln Ile Pro Ser Glu Gln Pro Gly Leu
945 950 955 960
Arg Lys Lys Ala Arg Lys Ile Ser Lys Ser Leu Lys Ser Arg Ile Lys
965 970 975
Gly Leu Phe Ser Arg Pro Lys Asn Arg Asp Asp Ile Val Asp Gln Thr
980 985 990
Glu Glu His Pro Gln Asp Leu Glu Ser Asn Asn Gly Ser Ser Tyr Ser
995 1000 1005
Ser Gln Thr Pro Ala Arg Leu Ser His Glu Ala Ser Leu Tyr Arg
1010 1015 1020
Val Ile Ser His Met Pro Ser Phe His Ala Val Pro Pro Ser Gln
1025 1030 1035
Gln Leu Arg Ser Arg Gln Gly Ser Leu Gly Thr Ile Glu Val Glu
1040 1045 1050
Asp Asn Thr Leu Asp Asp Asp Lys Ser Arg Val Thr Ser Trp Thr
1055 1060 1065
Asn Ser Thr Ala Asp Thr Ile Ser Asn Gln Leu Ser Ala Asn Trp
1070 1075 1080
Ser Arg Gln Tyr Pro Ser Leu Leu Gln Asp Asp Met Asp Thr Thr
1085 1090 1095
Leu Ser Pro His Asn Gln Thr Ser Met Tyr Arg Lys Asn Met Ile
1100 1105 1110
Asn Thr Gly Pro Ile Val Asn Ser Gln Arg Val Tyr Ser Ala Leu
1115 1120 1125
Met Lys Arg Leu Gln Glu Lys Asn Gly His Gln Asn Gln Ser Leu
1130 1135 1140
Gly Glu Asp His Met Thr Pro Asp Pro Val Glu Thr Asn Ser Leu
1145 1150 1155
Arg Ser Thr Ser Thr Ile Arg Arg Gln Thr Arg Gln Ala Met Tyr
1160 1165 1170
Ala Gly Ser Thr His Thr Ile Arg His Val Gln Tyr Asp Asp Asp
1175 1180 1185
Val Phe Gln Asp Ser Val Glu Gly Ser Thr Thr Ser Arg Tyr Ser
1190 1195 1200
Ser Glu Ser Thr Lys Ser Val Ile Arg Arg His Gln Ser Ser Asp
1205 1210 1215
Ser Leu Gln Asp Tyr Gly His Lys Ser Cys Pro Ser Pro Gly Ser
1220 1225 1230
Gly Asn Val Ser Gly Phe Pro Pro Asn Thr Asn Asp Val Lys Pro
1235 1240 1245
His Pro Val Thr Glu Asn Gln Lys Pro Thr Leu Ser Gly Arg Ser
1250 1255 1260
Ser Ala Phe Phe Ala Ser Pro Thr Cys His Leu Phe Arg Thr Thr
1265 1270 1275
Ser Pro Tyr Arg Arg Ala Leu Gln Glu Ser Met Arg Ala Ala Gln
1280 1285 1290
Ala Ser Asn Gln Pro Gln Thr Pro Gly Thr Lys His Leu Ser Ser
1295 1300 1305
Leu Ser Ala Leu Ser Leu Pro Ile Arg Arg Pro Ser Thr Glu Glu
1310 1315 1320
Ser Asp Asp Asp Ala Arg Val Ala Tyr Ala Glu Ser Val Tyr Ser
1325 1330 1335
Asn Ser Ser Glu Glu Asn Ala Pro Gln Val Asp Glu Pro Ser Pro
1340 1345 1350
Pro Leu Arg Ser Thr Glu Glu Glu Pro Arg Pro Gln Val His Gly
1355 1360 1365
Asp Ala Thr Ile Phe Leu Asn Thr Glu Arg Pro Arg Leu Pro Pro
1370 1375 1380
Pro Ser His Thr Arg Asp Ser Ser Ser Ala Ser Ser Val Glu Trp
1385 1390 1395
Lys Thr Trp Leu Ser Ala Asn Val Ser Lys Leu Glu Thr Pro Pro
1400 1405 1410
Thr Thr Leu Lys Pro Asp Leu Ser Gln Glu Pro Ser Ser Ala Pro
1415 1420 1425
Gln Thr Phe Ser His Val Arg Glu Lys Thr Glu Ile Asp Ser Asp
1430 1435 1440
Val Asp Asp Tyr Pro Gln Asp Ala Pro Gly Gly Gly Leu Ser Pro
1445 1450 1455
Lys Leu Gly Leu Ser Lys Asp Asn His Ser Pro Ser Val Leu Thr
1460 1465 1470
Pro Ile Pro Arg Gly Lys Phe His Leu Val Asn Pro Trp His Leu
1475 1480 1485
Gln Ser Thr Ala Ser Asn Glu Asn Ser Pro Pro Thr Ser Gly Val
1490 1495 1500
Arg Ser Lys Ser Ala Cys Ser Lys Asp Ala Ser Pro Val Ala Pro
1505 1510 1515
Lys Gly His Leu Arg Thr Ile Pro Ser Val Pro Asn Val Asn Thr
1520 1525 1530
Cys Ala Thr Asn Ala Glu Ser Leu Met Gly Glu Pro Arg Asn Pro
1535 1540 1545
Ser Leu Asn Thr Cys Ser Lys Pro Phe Asp Pro Val Arg Glu Glu
1550 1555 1560
Ala Arg Leu Lys Arg Arg Ser Arg Thr Arg Leu Gly Ala Asn Asp
1565 1570 1575
Ala Ser Ala Lys Ser Ser Pro Gly Phe Thr Thr Ala Leu Lys Arg
1580 1585 1590
Gln Phe Gly Val Ser Arg Thr Gly Ser Pro Gly Val Trp Lys Ala
1595 1600 1605
Glu Glu Pro Ser Gly Glu Leu Gly Ser Glu Ala Asp Pro Gly Val
1610 1615 1620
Asp Glu Leu Gly Arg Arg Asp Phe Asp Ala Gln Ala Met Gly Ser
1625 1630 1635
Arg Arg Met Val Asp Leu Phe Leu Ser Ser Arg Arg Gln Arg Ile
1640 1645 1650
Ala Gly Ser Gln Thr Arg Gln Ser Ser Asp Ser Leu Ser Ala Ala
1655 1660 1665
Phe Ile
1670
<210> 101
<211> 538
<212> PRT
<213> 有性哈茨木霉
<400> 101
Met Arg Thr Ala Val Ala Leu Ser Val Ala Ala Val Ala Gln Ala Ser
1 5 10 15
Thr Phe Gln Ile Gly Thr Ile His Glu Asn Ser Ala Pro Val Leu Ser
20 25 30
Thr Val Glu Ala Asn Ala Ile Pro Asp Ala Tyr Ile Ile Lys Phe Lys
35 40 45
Asp His Val Gly Glu Ala Gln Ala Glu Lys His His Asp Trp Leu Gln
50 55 60
Ser Val His Ser Ser Gly Glu Gln Glu Arg Leu Glu Leu Arg Lys Arg
65 70 75 80
Ser Ser Ile Phe Gly Ser Asp Asp Val Phe Asp Gly Leu Lys His Thr
85 90 95
Phe Lys Met Asp Gly Phe Lys Gly Tyr Ala Gly His Phe His Glu Ser
100 105 110
Thr Ile Glu Gln Val Arg Asn His Pro Asp Val Glu Tyr Ile Glu Arg
115 120 125
Asp Ser Ile Val His Thr Met Leu Pro Leu Glu Ser Lys Asp Asn Ile
130 135 140
Ile Ile Glu Asp Ser Cys Ser Pro Glu Thr Glu Lys Gln Ala Pro Trp
145 150 155 160
Gly Leu Ala Arg Ile Ser His Arg Asp Thr Leu Asn Phe Gly Ser Phe
165 170 175
Asn Lys Tyr Leu Tyr Thr Ala Glu Gly Gly Glu Gly Val Asp Ala Tyr
180 185 190
Ile Ile Asp Thr Gly Thr Asn Ile Glu His Val Asp Phe Glu Gly Arg
195 200 205
Ala Lys Trp Gly Lys Thr Ile Pro Ala Gly Asp Glu Asp Glu Asp Gly
210 215 220
Asn Gly His Gly Thr His Cys Ser Gly Thr Val Ala Gly Lys Lys Tyr
225 230 235 240
Gly Val Ala Lys Lys Ala His Val Tyr Ala Val Lys Val Leu Arg Ser
245 250 255
Asn Gly Ser Gly Thr Met Ser Asp Val Val Lys Gly Val Glu Phe Ala
260 265 270
Ala Ile Ser His Gln Glu Ala Val Lys Gln Ala Lys Asp Gly Lys Arg
275 280 285
Lys Gly Phe Lys Gly Ser Val Ala Asn Met Ser Leu Gly Gly Gly Lys
290 295 300
Thr Ser Ala Leu Asp Ala Ala Val Asn Ala Ala Val Lys Ala Gly Ile
305 310 315 320
His Phe Ala Val Ala Ala Gly Asn Asp Asn Ala Asp Ala Cys Asn Tyr
325 330 335
Ser Pro Ala Ala Ala Ser Glu Pro Val Thr Val Gly Ala Ser Ala Leu
340 345 350
Asp Asp Ser Arg Ala Tyr Phe Ser Asn Tyr Gly Lys Cys Thr Asp Ile
355 360 365
Phe Ala Pro Gly Leu Ser Ile Gln Ser Thr Trp Ile Gly Ser Lys Tyr
370 375 380
Ala Val Asn Thr Ile Ser Gly Thr Ser Met Ala Ser Pro His Ile Ala
385 390 395 400
Gly Leu Leu Ala Tyr Tyr Leu Ser Leu Gln Pro Ala Thr Asp Ser Glu
405 410 415
Phe Ala Val Ala Pro Ile Thr Pro Asn Lys Leu Lys Lys Asp Ile Ile
420 425 430
Ser Val Ala Thr Val Gly Thr Leu Ser Asp Ile Pro Ser Asp Thr Pro
435 440 445
Asn Leu Leu Ala Trp Asn Gly Ala Gly Cys Ser Asn Tyr Ser Gln Ile
450 455 460
Ile Glu Ala Gly Ser Tyr Thr Ala Lys Pro Lys Ala Asn Lys Ala Lys
465 470 475 480
Leu Pro Ala Thr Ile Glu Glu Leu Glu Glu Ala Ile Glu Gly Asp Phe
485 490 495
Glu Val Val Ser Gly Lys Ile Val Lys Gly Ala Lys Thr Phe Gly Ser
500 505 510
Lys Ala Glu Lys Phe Ala Lys Lys Ile His Asp Leu Val Glu Glu Glu
515 520 525
Ile Glu Glu Phe Ile Ser Glu Leu Ser Glu
530 535
<210> 102
<211> 495
<212> PRT
<213> 烟曲霉
<400> 102
Met Lys Gly Tyr Leu Ser Leu Ser Ile Leu Pro Leu Leu Val Ala Ala
1 5 10 15
Ser Pro Val Val Val Asp Ser Ile His Asn Gly Ala Ala Pro Ile Leu
20 25 30
Ser Ser Met Asn Ala Lys Glu Val Pro Asp Ser Tyr Ile Val Val Phe
35 40 45
Lys Lys His Val Asn Ala Glu Ser Ala Ala Ala His His Ser Trp Val
50 55 60
Gln Asp Ile His Ser Ala Gln Asn Glu Arg Val Glu Leu Arg Lys Arg
65 70 75 80
Ser Leu Phe Gly Phe Gly Glu Glu Ala Tyr Leu Gly Leu Lys Asn Thr
85 90 95
Phe Asp Ile Ala Gly Ser Leu Val Gly Tyr Ser Gly His Phe His Glu
100 105 110
Asp Val Ile Glu Gln Val Arg Lys His Pro Asp Val Glu Tyr Ile Glu
115 120 125
Lys Asp Ser Glu Val His Thr Met Glu Asp Pro Thr Val Glu Lys Ser
130 135 140
Ala Pro Trp Gly Leu Ala Arg Ile Ser His Arg Asp Ser Leu Ser Phe
145 150 155 160
Gly Thr Phe Asn Lys Tyr Leu Tyr Ala Ser Glu Gly Gly Glu Gly Val
165 170 175
Asp Ala Tyr Thr Ile Asp Thr Gly Ile Asn Val Asp His Val Asp Phe
180 185 190
Glu Gly Arg Ala Gln Trp Gly Lys Thr Ile Pro Thr Asp Asp Glu Asp
195 200 205
Ala Asp Gly Asn Gly His Gly Thr His Cys Ser Gly Thr Ile Ala Gly
210 215 220
Arg Lys Tyr Gly Val Ala Lys Lys Ala Asn Leu Tyr Ala Val Lys Val
225 230 235 240
Leu Arg Ser Ser Gly Ser Gly Thr Met Ser Asp Val Val Ala Gly Val
245 250 255
Glu Trp Ala Val Lys Ser His Leu Lys Lys Val Lys Asp Ala Lys Asp
260 265 270
Gly Lys Ile Lys Gly Phe Lys Gly Ser Val Ala Asn Met Ser Leu Gly
275 280 285
Gly Gly Lys Ser Arg Thr Leu Glu Ala Ala Val Asn Ala Gly Val Glu
290 295 300
Ala Gly Leu His Phe Ala Val Ala Ala Gly Asn Asp Asn Ala Asp Ala
305 310 315 320
Cys Asn Tyr Ser Pro Ala Ala Ala Glu Asn Pro Ile Thr Val Gly Ala
325 330 335
Ser Thr Leu Gln Asp Glu Arg Ala Tyr Phe Ser Asn Tyr Gly Lys Cys
340 345 350
Thr Asp Ile Phe Ala Pro Gly Leu Asn Ile Leu Ser Thr Trp Ile Gly
355 360 365
Ser Lys His Ala Val Asn Thr Ile Ser Gly Thr Ser Met Ala Ser Pro
370 375 380
His Ile Ala Gly Leu Leu Ala Tyr Phe Val Ser Leu Gln Pro Ser Lys
385 390 395 400
Asp Ser Ala Phe Ala Val Asp Glu Leu Thr Pro Lys Lys Leu Lys Lys
405 410 415
Asp Ile Ile Ala Ile Ala Thr Gln Gly Ala Leu Thr Asp Ile Pro Ser
420 425 430
Asp Thr Pro Asn Leu Leu Ala Trp Asn Gly Gly Gly Ser Ser Asn Tyr
435 440 445
Thr Asp Ile Ile Ala Ser Gly Gly Tyr Lys Val Asn Ala Ser Val Lys
450 455 460
Asp Arg Phe Glu Gly Leu Val His Lys Ala Glu Lys Leu Leu Thr Glu
465 470 475 480
Glu Leu Gly Ala Ile Tyr Ser Glu Ile His Asp Ala Ala Val Ala
485 490 495
<210> 103
<211> 530
<212> PRT
<213> Nectria haematococca
<400> 103
Met Arg Thr Ala Leu Thr Leu Ser Leu Ala Ala Val Ala Asn Ala Ala
1 5 10 15
Ser Phe Ser Val Gly Thr Ile His Asp Arg Ser Ala Pro Ile Leu Ser
20 25 30
Ser Ile Glu Ala Glu Thr Ile Pro Asp Ser Tyr Ile Ile Lys Phe Lys
35 40 45
Asp His Val Asp Glu Thr Ala Ala Ser Asp His His Thr Trp Ile Gln
50 55 60
Asn Ile His Ser His Gly Glu Gln Glu Arg Leu Glu Leu Arg Lys Arg
65 70 75 80
Ser Thr Pro Phe Thr Asp Lys Thr Phe Ala Gly Leu Lys His Thr Phe
85 90 95
Asn Ile Gly Lys Gly Phe Lys Gly Tyr Ala Gly His Phe Asp Glu Ser
100 105 110
Val Ile Glu Gln Val Arg Asn His Pro Asp Ile Glu Phe Ile Glu Arg
115 120 125
Asp Thr Leu Val His Thr Met Ile Pro Val Gln Asn Gln Asp Ala Val
130 135 140
Ser Glu Tyr Lys Cys Asp Asp Gly Lys Thr Glu Thr Ser Ala Pro Trp
145 150 155 160
Gly Leu Ala Arg Ile Ser His Arg Asp Thr Leu Asn Phe Gly Thr Phe
165 170 175
Asn Lys Tyr Leu Tyr Ser Ala Asp Gly Gly Glu Gly Val Asp Ala Tyr
180 185 190
Ile Val Asp Thr Gly Thr Asn Ile Asp His Val Asp Phe Glu Gly Arg
195 200 205
Ala His Trp Gly Lys Thr Val Pro Ala Gly Asp Ala Asp Glu Asp Gly
210 215 220
Asn Gly His Gly Thr His Cys Ser Gly Thr Val Ala Gly Lys Arg Tyr
225 230 235 240
Gly Val Ala Lys Lys Ala Asn Val Tyr Ala Val Lys Val Leu Arg Ser
245 250 255
Asn Gly Ser Gly Ser Met Ser Asp Val Val Lys Gly Val Glu Phe Ala
260 265 270
Ala Thr Ser His Leu Glu Gln Ala Gln Lys Ala Lys Asp Gly Lys Arg
275 280 285
Lys Gly Phe Lys Gly Ser Val Ala Asn Met Ser Leu Gly Gly Gly Lys
290 295 300
Thr Gln Ala Leu Asp Ala Ala Val Asn Ala Ala Val Arg Thr Gly Val
305 310 315 320
His Phe Ala Val Ala Ala Gly Asn Asp Asn Ala Asp Ala Cys Asn Tyr
325 330 335
Ser Pro Ala Ala Ala Ala Glu Pro Val Thr Val Gly Ala Ser Ala Leu
340 345 350
Asp Asp Ser Arg Ala Tyr Phe Ser Asn Tyr Gly Lys Cys Thr Asp Ile
355 360 365
Phe Ala Pro Gly Leu Asn Ile Gln Ser Thr Trp Ile Gly Ser Lys Tyr
370 375 380
Ala Ile Asn Thr Ile Ser Gly Thr Ser Met Ala Ser Pro His Ile Ala
385 390 395 400
Gly Leu Leu Ala Tyr Tyr Leu Ser Leu Gln Pro Ala Glu Asp Ser Glu
405 410 415
Tyr Ala Met Ala Ser Ile Thr Pro Lys Lys Leu Lys Glu Asn Leu Ile
420 425 430
Ser Ile Ala Thr Glu Asn Ala Leu Ser Asp Ile Pro Ser Asp Thr Pro
435 440 445
Asn Leu Leu Ala Trp Asn Gly Gly Gly Cys Ser Asp Tyr Lys Lys Ile
450 455 460
Val Glu Ala Gly Ser Tyr Gln Val Asn Lys Ala Ser Pro Ser Ser Pro
465 470 475 480
Ala Lys Gln Ile Lys Asp Ala Val Glu Gln Glu Ile Gly Val Val Ser
485 490 495
Gly Lys Val Lys Glu Phe Gly Ser Lys Ala Glu Lys Phe Ser Lys Lys
500 505 510
Ile His Asn Leu Val Asp Glu Glu Leu Glu Gln Phe Leu Lys Glu Ile
515 520 525
Asn Met
530
<210> 104
<211> 533
<212> PRT
<213> 玉米赤霉
<400> 104
Met Lys Ser Ala Leu Thr Leu Ser Met Ala Ala Val Ala Ser Ala Ala
1 5 10 15
Ser Phe Ser Val Gly Thr Val His Asp Lys Ala Ala Pro Ile Leu Ser
20 25 30
Ser Val Asp Ala Glu Thr Ile Pro Asp Ser Tyr Ile Ile Lys Phe Lys
35 40 45
Asp His Val Asp Glu His Ala Ala Ser Asn His His Met Trp Val Gln
50 55 60
Asp Thr His Thr Gln Gly Glu Ser Glu Arg Met Glu Leu Arg Lys Arg
65 70 75 80
Ser Ile Pro Phe Thr Asp Lys Thr Phe Ser Gly Leu Lys His Thr Phe
85 90 95
Asp Ile Gly Glu Ala Phe Lys Gly Tyr Ala Gly His Phe Asp Glu Ser
100 105 110
Met Ile Glu Lys Val Arg Asn His Pro Asp Val Glu Phe Ile Glu Arg
115 120 125
Asp Thr Leu Val His Thr Met Val Pro Ile Thr Asn Asn Asp Met Val
130 135 140
Thr Glu Asp Lys Cys Asp Gly Glu Thr Glu Arg Ser Ala Pro Trp Gly
145 150 155 160
Leu Ala Arg Ile Ser His Arg Asn Thr Leu Asn Phe Gly Ser Phe Asn
165 170 175
Lys Tyr Leu Tyr Ser Ser Asp Gly Gly Glu Gly Val Asp Ala Tyr Ile
180 185 190
Val Asp Thr Gly Thr Asn Ile Lys His Val Asp Phe Glu Gly Arg Ala
195 200 205
His Trp Gly Lys Thr Ile Pro Ser Gly Asp Ala Asp Glu Asp Gly Asn
210 215 220
Gly His Gly Thr His Cys Ser Gly Thr Val Ala Gly Lys Lys Tyr Gly
225 230 235 240
Val Ala Lys Lys Ala Ser Val Tyr Ala Val Lys Val Leu Arg Ser Asn
245 250 255
Gly Ser Gly Ser Met Ser Asp Val Val Lys Gly Val Glu Phe Ala Ala
260 265 270
Thr Ser His Leu Glu Gln Lys Lys Lys Ala Lys Asp Gly Lys Arg Lys
275 280 285
Gly Phe Lys Gly Ser Val Ala Asn Met Ser Leu Gly Gly Gly Lys Thr
290 295 300
Gln Ala Leu Asp Ala Ala Val Asn Ala Ala Val Arg Thr Gly Ile His
305 310 315 320
Phe Ala Val Ala Ala Gly Asn Asp Asn Ala Asp Ala Cys Asn Tyr Ser
325 330 335
Pro Ala Ala Ala Ser Glu Pro Val Thr Val Gly Ala Ser Ala Ile Asp
340 345 350
Asp Ser Arg Ala Tyr Phe Ser Asn Tyr Gly Lys Cys Thr Asp Ile Phe
355 360 365
Ala Pro Gly Leu Asn Ile Val Ser Thr Trp Ile Gly Thr Asp Lys Ala
370 375 380
Ile Asn Thr Ile Ser Gly Thr Ser Met Ala Ser Pro His Ile Ala Gly
385 390 395 400
Leu Leu Ala Tyr Tyr Leu Ser Leu Gln Pro Ala Glu Asp Ser Glu Tyr
405 410 415
Ala Leu Ala Ser Ile Thr Pro Lys Lys Leu Lys Glu Asn Leu Ile Ser
420 425 430
Val Ala Thr Glu Asn Ala Leu Ser Asp Ile Pro Ser Asp Thr Pro Asn
435 440 445
Leu Leu Ala Trp Asn Gly Gly Gly Cys Ser Asp Tyr Lys Lys Ile Val
450 455 460
Glu Ala Gly Ser Tyr Lys Val Asn Lys Ala Ala Pro Ser Ser Arg Val
465 470 475 480
Glu Asp Ile Lys His Ala Val Glu Gln Glu Val Glu Val Val Ser Gly
485 490 495
Lys Leu Thr Thr Gly Ala Lys Gln Leu Gly Ser Lys Ala Glu Lys Phe
500 505 510
Ser Lys Lys Ile His Glu Leu Val Asp Glu Glu Leu Glu Gln Phe Ile
515 520 525
Lys Glu Leu Asn Leu
530
<210> 105
<211> 532
<212> PRT
<213> 虫花棒束孢
<400> 105
Met Lys Ser Phe Val Ala Ala Leu Ser Val Ala Ala Ile Ala Gln Ala
1 5 10 15
Ser Ser Phe Lys Ile Gly Thr Ile His His Asp Ser Ala Pro Ile Leu
20 25 30
Asp Ala Thr His Ala Asn Thr Ile Pro Gly Ala Tyr Ile Ile Lys Phe
35 40 45
Lys Glu His Val Asp Glu Pro Lys Ala Lys Thr His His Ala Trp Val
50 55 60
Thr Asp Ile His Gly Asp Gly Gln Gln Gln Arg Ile Glu Leu Arg Lys
65 70 75 80
Arg Gly Leu Phe Asp Ser Val Glu Asp Val Phe Asp Gly Val Lys His
85 90 95
Thr Phe Asp Leu Gly Ala Gly Phe Arg Gly Tyr Ala Gly His Phe Asp
100 105 110
Asp Ala Val Ile Glu Gln Ile Arg Asn His Pro Asp Val Glu Ala Ile
115 120 125
Glu Arg Asp Ser Ile Val His Thr Met Val Pro Val Ser Ala Lys Asp
130 135 140
Thr Val Thr Glu Asp Thr Cys Asn Pro Glu Thr Glu Lys Gln Ala Pro
145 150 155 160
Trp Gly Leu Ala Arg Ile Ser His Arg Lys Thr Leu Gly Phe Gly Thr
165 170 175
Phe Asn Lys Tyr Leu Tyr Ala Glu Asp Gly Gly Glu Gly Val Asp Ala
180 185 190
Tyr Val Ile Asp Thr Gly Thr Asn Ile Ala His Val Asp Phe Glu Gly
195 200 205
Arg Ala Lys Trp Gly Tyr Thr Ala Pro Asp Gly Asp Ala Asp Glu Asp
210 215 220
Gly Asn Gly His Gly Thr His Cys Ser Gly Thr Ile Ala Gly Lys Lys
225 230 235 240
Tyr Gly Val Ala Lys Lys Ala Asn Val Tyr Ala Val Lys Val Leu Arg
245 250 255
Ser Asn Gly Ser Gly Thr Met Ser Asp Val Ile Lys Gly Val Asp Trp
260 265 270
Ala Ala Lys Ser His Glu Gln Gln Val Lys Asp Ala Lys Ala Gly Lys
275 280 285
Arg Lys Gly Phe Lys Gly Ser Val Ala Asn Met Ser Leu Gly Gly Gly
290 295 300
Lys Thr Thr Leu Leu Asp Ile Ala Val Asp Ala Ala Val Ala Lys Gly
305 310 315 320
Leu His Phe Ala Val Ala Ala Gly Asn Asp Asn Ala Asp Ala Cys Asn
325 330 335
Tyr Ser Pro Ala Ala Ala Lys Asn Ala Val Thr Val Gly Ala Ser Thr
340 345 350
Ile Asp Asp Ser Arg Ala Tyr Phe Ser Asn Tyr Gly Lys Cys Thr Asp
355 360 365
Ile Phe Ala Pro Gly Leu Asn Ile Gln Ser Thr Trp Ile Gly Ser Lys
370 375 380
Tyr Ala Ile Asn Thr Ile Ser Gly Thr Ser Met Ala Ser Pro His Ile
385 390 395 400
Cys Gly Leu Leu Ala Tyr Tyr Leu Ser Leu Gln Pro Ala Ser Asp Ser
405 410 415
Glu Tyr Ala Val Ala Ser Ile Thr Pro Ala Glu Leu Lys Asp Asn Leu
420 425 430
Ile Thr Ile Gly Thr Lys Asn Thr Leu Ser Asp Ile Asp Ser Asp Thr
435 440 445
Pro Asn Val Leu Ala Trp Asn Gly Ala Gly Cys Ser Asp Tyr Lys Lys
450 455 460
Ile Val Glu Ala Gly Ala Tyr Asp Ala Lys Arg Thr Ser Ser Lys Ser
465 470 475 480
Glu Asn Ile His Thr Lys Ile Gln His Glu Phe Glu Ile Val Ser Gly
485 490 495
Glu Ile Thr Lys Ser Ala Lys Thr Phe Gly Ser Lys Ala Glu Lys Phe
500 505 510
Ala Lys Lys Ile Gln His Ile Val Asp Ala Glu Val Glu Asp Phe Leu
515 520 525
Ser His Met Glu
530
<210> 106
<211> 537
<212> PRT
<213> Glomerella graminicola
<400> 106
Met Arg Gly Phe Ile Gly Ala Val Ala Gly Leu Ala Ala Val Ala Ser
1 5 10 15
Ala Thr Pro Ser Leu Ser Ile Glu Thr Ile His Asp Gly Ala Ala Pro
20 25 30
Val Ile Ser Ser Thr Asn Ala Glu Ala Val Pro Asn Ser Tyr Ile Ile
35 40 45
Lys Phe Lys Lys His Val Ser Asp Thr Asp Ala Glu Asp His His Asn
50 55 60
Trp Val Gln Gln Ile His Ser Ser Ser Gln Asp Glu Arg Leu Glu Leu
65 70 75 80
Arg Lys Arg Gly Gln Glu Pro Leu Val Asp Asn Val Phe His Gly Leu
85 90 95
Lys His Thr Tyr Lys Ile Gly Asn Asp Phe Leu Gly Tyr Ala Gly His
100 105 110
Phe Asp Glu Ala Thr Ile Glu Lys Ile Arg Arg His Ser Asp Val Glu
115 120 125
Phe Ile Glu Val Asp Ser Val Val His Thr Gln Val Pro Val Lys Ser
130 135 140
Gln Gly Ala Glu Ala Glu Asp Thr Cys Asp Gly Glu Leu Glu Lys Leu
145 150 155 160
Ala Pro Trp Gly Leu Ala Arg Val Ser His Arg Lys Ser Leu Gly Phe
165 170 175
Gly Thr Tyr Asp Lys Tyr Leu Tyr Ala Glu Asp Ala Gly Glu Gly Val
180 185 190
Asp Ala Tyr Val Ile Asp Thr Gly Thr Asn Val Asp His Val Asp Phe
195 200 205
Glu Gly Arg Ala Lys Trp Gly Lys Thr Ile Pro Ser Gly Asp Gln Asp
210 215 220
Val Asp Gly Asn Gly His Gly Thr His Cys Ser Gly Thr Ile Ala Gly
225 230 235 240
Lys Lys Tyr Gly Val Ala Lys Lys Ala Asn Val Tyr Ala Val Lys Val
245 250 255
Leu Arg Ser Asn Gly Ser Gly Thr Met Ser Asp Val Val Lys Gly Val
260 265 270
Glu Trp Ala Ala Thr Ser His Ile Glu Gln Val Lys Ala Ala Lys Asp
275 280 285
Gly Lys Arg Lys Gly Phe Lys Gly Ser Val Ala Asn Met Ser Leu Gly
290 295 300
Gly Gly Lys Thr Gln Ala Leu Asp Ala Val Val Asn Ala Ala Val Asp
305 310 315 320
Ala Gly Ile His Phe Ala Val Ala Ala Gly Asn Asp Asn Ala Asp Ala
325 330 335
Cys Asn Tyr Ser Pro Ala Ala Ala Glu Lys Ala Val Thr Val Gly Ala
340 345 350
Thr Glu Leu Gly Asp Arg Arg Ser Tyr Phe Ser Asn Tyr Gly Lys Cys
355 360 365
Thr Asp Ile Phe Ala Pro Gly Thr Asn Ile Lys Ser Thr Trp Ile Gly
370 375 380
Ser Lys Tyr Ala Val Asn Thr Ile Ser Gly Thr Ser Met Ala Ser Pro
385 390 395 400
His Ile Cys Gly Leu Leu Ala Tyr Tyr Leu Ser Leu Gln Pro Ser Thr
405 410 415
Asp Ser Glu Tyr Ser Val Ala Pro Ile Thr Pro Gln Lys Leu Lys Lys
420 425 430
Ala Leu Ile Ser Val Gly Thr Val Gly Ala Leu Ala Asp Ile Pro Arg
435 440 445
Asp Thr Pro Asn Val Leu Ala Trp Asn Gly Gly Gly Cys Ser Asn Tyr
450 455 460
Ser Ala Ile Ile Glu Ala Gly Ser Tyr Thr Val Lys Gln Glu Thr Lys
465 470 475 480
Glu Ala Thr Ile Asp Asp Leu Glu Lys Ala Ile Glu Glu Asp Phe Glu
485 490 495
Val Leu Ser Gly Lys Val Val Lys Gly Ala Lys Asn Ile Gly Thr Lys
500 505 510
Ala Glu Lys Leu Ala Lys Lys Ile His Asn Leu Val Asp Glu Glu Leu
515 520 525
Lys Glu Phe Leu Arg Glu Thr Ser Leu
530 535
<210> 107
<211> 531
<212> PRT
<213> Epichloe festucae
<400> 107
Met Lys Thr Ile Leu Ala Leu Ser Val Ala Ala Val Ala Gln Ala Ala
1 5 10 15
Phe Ser Thr Glu Thr Ile His Lys Gly Ser Ala Pro Val Leu Ser Ser
20 25 30
Ile His Ala Asp Val Val Pro Asp Ser Tyr Ile Ile Lys Phe Lys Asp
35 40 45
His Val Asp Glu Ala Lys Ala Asn Glu His His Ala Trp Val Lys Asp
50 55 60
Ile His Thr Gly Gly Glu Glu Asp Arg Phe Glu Leu Arg Lys Arg Gly
65 70 75 80
Leu Gly Ser Ser Ile Val Asp Ala Phe Ser Gly Thr Lys His Thr Phe
85 90 95
Asn Phe Asp Gly Phe Lys Gly Tyr Ser Gly His Phe His Glu Ser Val
100 105 110
Ile Glu Lys Val Arg Asn Asn Pro Asp Val Glu Phe Ile Glu Lys Asp
115 120 125
Thr Val Val His Thr Met Leu Pro Leu Thr Ser Glu His Ser Val Gln
130 135 140
Glu Asp Lys Cys Asp Pro Gly Glu Thr Glu Lys Gln Ala Pro Trp Gly
145 150 155 160
Leu Ala Arg Leu Ser His Arg Asp Ser Leu Arg Phe Ser Thr Tyr Asn
165 170 175
Lys Tyr Leu Tyr Ala Thr Glu Gly Gly Glu Gly Val Asp Ala Tyr Val
180 185 190
Ile Asp Thr Gly Thr Asn Val Asp His Val Asp Phe Glu Gly Arg Ala
195 200 205
Lys Trp Gly Lys Thr Ile Pro Ser Gly Asp Ala Asp Glu Asp Gly Asn
210 215 220
Gly His Gly Thr His Cys Ser Gly Thr Ile Ala Gly Lys Lys Tyr Gly
225 230 235 240
Val Ala Lys Lys Ala His Val Tyr Ala Val Lys Val Leu Arg Ser Asn
245 250 255
Gly Ser Gly Ser Met Ser Asp Val Val Lys Gly Val Glu Phe Ala Ala
260 265 270
Asn Ser His Thr Glu Gln Val Lys Ala Ala Lys Asp Gly Lys Arg Lys
275 280 285
Gly Phe Lys Gly Ser Val Ala Asn Met Ser Leu Gly Gly Gly Lys Thr
290 295 300
Ser Ala Leu Asp Ala Ala Val Asn Ala Ala Val Lys Val Gly Ile His
305 310 315 320
Phe Ala Val Ala Ala Gly Asn Asp Asn Ala Asp Ala Cys Asp Tyr Ser
325 330 335
Pro Ala Ala Ala Glu His Pro Val Thr Val Gly Ala Ser Ala Phe Asp
340 345 350
Asp Ser Arg Ala Tyr Phe Ser Asn Tyr Gly Lys Cys Thr Asp Ile Phe
355 360 365
Ala Pro Gly Leu Asn Ile Leu Ser Thr Trp Ile Gly Ser Lys Tyr Ala
370 375 380
Val Asn Thr Ile Ser Gly Thr Ser Met Ala Ser Pro His Ile Cys Gly
385 390 395 400
Leu Leu Ala Tyr Tyr Leu Ser Leu Gln Pro Ala Lys Asp Ser Glu Tyr
405 410 415
Ser Val Ala Ala Met Thr Pro Lys Asp Leu Lys Asp Ala Leu Ile Glu
420 425 430
Val Ser Thr Glu Gly Val Leu Ser Asp Ile Pro Ser Asp Thr Pro Asn
435 440 445
Lys Leu Ala Trp Asn Gly Gly Gly Cys Asn Asn Tyr Ser Lys Ile Val
450 455 460
Ala Ala Gly Gly Tyr Lys Ala Lys Ser Glu Ala Ser Glu Leu Lys Lys
465 470 475 480
Ile Glu Lys Val Ala Met Asp Glu Ala Lys Glu Ile Ser Gly Lys Val
485 490 495
Val Lys Gly Val Lys His Leu Gly Glu Lys Thr Glu Lys Phe Val Glu
500 505 510
Lys Val His Ser Val Val Asp Lys Glu Ile Glu His Phe Ile Ser Glu
515 520 525
Ile Ser Ser
530
<210> 108
<211> 533
<212> PRT
<213> Metarhizium acridum
<400> 108
Met His Thr Leu Val Ala Ala Val Ala Leu Ser Val Val Gly Val Ala
1 5 10 15
Gln Ala Gly Phe Ser Ile Glu Thr Ile His Lys Glu Ser Ala Pro Ile
20 25 30
Leu Ser Ser Val Asp Ala Glu Val Val Pro Asp Ser Tyr Ile Ile Lys
35 40 45
Phe Lys Asp His Val Asn Glu Ala Ala Ala Asn Asp His Arg Ser Trp
50 55 60
Ile Gln Ser Ile His Lys Asp Gly Glu Glu Glu Arg Leu Glu Leu Arg
65 70 75 80
Lys Arg Ser Leu Gly Ser Ser Pro Val Glu Ala Phe Ala Gly Leu Lys
85 90 95
His Thr Tyr Asn Ile Ala Asn Gly Phe Lys Gly Tyr Ser Gly His Phe
100 105 110
His Glu Ser Val Ile Glu Lys Val Arg Asn His Arg Asp Val Glu Phe
115 120 125
Val Glu Lys Asp Thr Ile Ala Arg Ile Leu Arg Pro Val Ile Ser Asp
130 135 140
Glu Ser Ala Gln Glu Asp Cys Thr Pro Glu Thr Glu Arg Gln Ala Pro
145 150 155 160
Trp Gly Leu Ala Arg Val Ser His Arg Lys Gly Leu Ser Phe Gly Thr
165 170 175
Tyr Asn Lys Tyr Leu Tyr Ala Ala Asp Gly Gly Glu Gly Val Asp Ala
180 185 190
Tyr Val Ile Asp Thr Gly Thr Asn Thr Glu His Val Asp Phe Glu Gly
195 200 205
Arg Ala Lys Trp Gly Lys Thr Ile Pro Ala Gly Asp Ala Asp Glu Asp
210 215 220
Gly Asn Gly His Gly Thr His Cys Ser Gly Thr Ile Ala Gly Lys Lys
225 230 235 240
Tyr Gly Val Ala Lys Lys Ala Asn Val Tyr Ala Val Lys Val Leu Arg
245 250 255
Ser Asn Gly Ser Gly Thr Met Ala Asp Val Val Lys Gly Val Glu Phe
260 265 270
Ala Ala Thr Asn His Val Glu Gln Val Leu Leu Ala Lys Asp Gly Lys
275 280 285
Arg Lys Gly Phe Lys Gly Ser Val Ala Asn Met Ser Leu Gly Gly Gly
290 295 300
Lys Thr Gln Ala Leu Asp Ala Ala Val Asn Ala Ala Val Lys Ala Gly
305 310 315 320
Ile His Phe Ala Val Ala Ala Gly Asn Asp Asn Ala Asp Ala Cys Asn
325 330 335
Tyr Ser Pro Ala Ala Ala Glu Leu Pro Val Thr Val Gly Ala Ser Ala
340 345 350
Phe Asp Asp Ser Arg Ala Tyr Phe Ser Asn Tyr Gly Lys Cys Thr Asp
355 360 365
Ile Phe Ala Pro Gly Leu Asn Ile Leu Ser Thr Trp Ile Gly Ser Lys
370 375 380
Thr Ala Val Asn Thr Ile Ser Gly Thr Ser Met Ala Ser Pro His Ile
385 390 395 400
Cys Gly Leu Leu Ala Tyr Tyr Leu Ser Leu Gln Pro Ala Gly Asp Ser
405 410 415
Glu Phe Ser Val Ala Pro Ile Thr Pro Lys Gln Leu Lys Asp Thr Leu
420 425 430
Ile Glu Ile Ser Thr Gln Gly Val Leu Thr Asp Ile Pro Lys Asp Thr
435 440 445
Pro Asn Lys Leu Ala Trp Asn Gly Gly Gly Cys Ser Asn Tyr Ser Lys
450 455 460
Ile Val Ala Ala Gly Gly Trp Thr Gly Lys Ser Lys Thr Ser Glu Thr
465 470 475 480
Lys Pro Val Glu Asn Ala Val Lys Glu Glu Met Lys Val Leu Ser Asn
485 490 495
Lys Val Ser Glu Gly Val Lys Asp Leu Gly Asp Lys Thr Glu Lys Phe
500 505 510
Val Ala Lys Ile His Asp Val Val Asp Lys Glu Ile Glu His Phe Ile
515 520 525
Ser Glu Ile Ser Leu
530
<210> 109
<211> 535
<212> PRT
<213> 支顶孢属
<400> 109
Met Arg Ala Val Ile Ala Leu Ser Phe Ala Ala Tyr Ala Ser Ala Ala
1 5 10 15
Pro Arg Val Ser Phe Glu Thr Ile His Gly Asp Ala Ala Pro Ile Leu
20 25 30
Ser Ala Ser Asn Ala Glu Ile Ile Pro Asn Ser Tyr Ile Ile Lys Phe
35 40 45
Lys Lys His Val Thr Asp Asp Ser Ala Ser Ala His His Ser Trp Ile
50 55 60
Gln Glu Ile His Thr Ser Arg Glu Ser Glu Arg Ala Asp Leu Lys Lys
65 70 75 80
Arg Gly Gln Ile Pro Leu Val Asp Asp Val Phe Arg Gly Leu Lys His
85 90 95
Thr Tyr Lys Ile Gly Gln Glu Phe Leu Gly Tyr Ser Gly His Phe Asp
100 105 110
Asp Glu Val Ile Glu Gln Val Arg Arg His Pro Asp Val Glu Tyr Ile
115 120 125
Glu Arg Asp Ser Ile Val His Thr Met Arg Val Ala Glu Asn Ala Gln
130 135 140
Cys Asp Ser Glu Leu Glu Lys Ala Ala Pro Trp Gly Leu Ala Arg Ile
145 150 155 160
Ser His Arg Asn Thr Leu Ser Phe Ala Thr Phe Asn Lys Tyr Leu Phe
165 170 175
Ala Ala Glu Gly Gly Glu Gly Val Asp Ala Tyr Val Ile Asp Thr Gly
180 185 190
Thr Asn Ile Glu His Val Asp Phe Glu Gly Arg Ala Lys Trp Gly Lys
195 200 205
Thr Ile Pro Ser Gly Asp Ala Asp Val Asp Gly Asn Gly His Gly Thr
210 215 220
His Cys Ser Gly Thr Ile Ala Gly Lys Lys Tyr Gly Val Ala Lys Lys
225 230 235 240
Ala Asn Val Tyr Ala Val Lys Val Leu Arg Ser Asn Gly Ser Gly Thr
245 250 255
Met Ala Asp Val Val Ala Gly Val Glu Trp Ala Ala Lys Ala His Leu
260 265 270
Glu Gln Val Lys Ala Ala Lys Asp Gly Lys Arg Lys Gly Phe Lys Gly
275 280 285
Ser Val Ala Asn Met Ser Leu Gly Gly Gly Lys Thr Lys Ala Leu Asp
290 295 300
Asp Thr Val Asn Ala Ala Val Ser Val Gly Ile His Phe Ala Val Ala
305 310 315 320
Ala Gly Asn Asp Asn Ala Asp Ala Cys Asn Tyr Ser Pro Ala Ala Ala
325 330 335
Glu Lys Ala Val Thr Val Gly Ala Ser Ala Ile Asp Asp Ser Arg Ala
340 345 350
Tyr Phe Ser Asn Tyr Gly Lys Cys Thr Asp Ile Phe Ala Pro Gly Leu
355 360 365
Ser Ile Leu Ser Thr Trp Ile Gly Ser Lys Tyr Ala Thr Asn Thr Ile
370 375 380
Ser Gly Thr Ser Met Ala Ser Pro His Ile Cys Gly Leu Leu Ala Tyr
385 390 395 400
Tyr Leu Ser Leu Gln Pro Ala Ser Asp Ser Glu Tyr Ser Val Ala Pro
405 410 415
Ile Thr Pro Glu Lys Leu Lys Ser Asn Leu Leu Gln Ile Ala Thr Gln
420 425 430
Asp Ala Leu Thr Asp Met Pro Lys Gly Thr Pro Asn Leu Leu Ala Trp
435 440 445
Asn Gly Gly Gly Cys Asn Asn Tyr Thr Ala Ile Val Glu Ala Gly Gly
450 455 460
Tyr Lys Val Glu Arg Gln Ser Lys Thr Asp Lys Ile Asp Ile Gly Lys
465 470 475 480
Ser Val Ser Glu Leu Glu Lys Leu Ile Glu His Asp Phe Glu Val Val
485 490 495
Ser Gly Lys Val Val Lys Gly Val Ser Ser Phe Ala Gly Lys Ala Glu
500 505 510
Glu Phe Ser Lys Lys Ile His Gln Leu Val Asp Glu Glu Leu Lys Asp
515 520 525
Phe Leu Glu Asp Ile Ala Ala
530 535
<210> 110
<211> 503
<212> PRT
<213> Ophiostoma piliferum
<400> 110
Met Ser Leu Ala Ser Val Ala Val Ala Ala Pro Ser Phe Thr Thr Gly
1 5 10 15
Thr Val His Gly Asp Ala Ala Pro Ile Leu Ala Ser Val Asn Thr Glu
20 25 30
Ala Ile Pro Asn Ser Tyr Ile Val Lys Phe Lys Asn His Val Asn Glu
35 40 45
Lys Asp Val Ser Thr His His Ser Trp Val Gln Ser Leu His Asp Gln
50 55 60
Gly Glu Glu Lys Arg Leu Glu Leu Arg Arg Arg Gly Met Asp Phe Gly
65 70 75 80
Ile Leu Ser Gly Val Lys His Thr Phe Lys Ile Gly Glu Thr Phe Lys
85 90 95
Gly Tyr Ala Gly Gln Phe Asp Asp Glu Leu Ile Glu Gln Leu Arg Arg
100 105 110
His Pro Asp Val Glu Tyr Ile Glu Lys Asp Thr Val Val His Thr Met
115 120 125
Gln Pro Val Ser Ser Glu Glu Thr Cys Ser Ser Glu Val Glu Lys Asn
130 135 140
Ser Pro Trp Gly Leu Ala Arg Ile Ser His Arg Asp Thr Leu Ser Phe
145 150 155 160
Gly Thr Phe Asn Lys Tyr Leu Tyr Ser Ser Glu Gly Gly Glu Gly Val
165 170 175
Asp Val Tyr Val Ile Asp Thr Gly Cys Asn Val Asp His Val Asp Phe
180 185 190
Glu Gly Arg Ala Thr Trp Gly Lys Thr Ile Pro Ser Gly Val Ser Asp
195 200 205
Leu Asp Gly Asn Gly His Gly Thr His Cys Ser Gly Thr Ile Ala Gly
210 215 220
Lys Lys Tyr Gly Val Ala Lys Lys Ala Arg Val Tyr Ala Val Lys Val
225 230 235 240
Leu Arg Ser Asn Gly Ser Gly Thr Met Ser Asp Val Val Lys Gly Val
245 250 255
Glu Trp Ala Ala Asn Ala His Leu Glu Ala Leu Lys Gln Ala Lys Ala
260 265 270
Gly Lys Arg Lys Gly Phe Lys Gly Ser Ala Ala Asn Met Ser Leu Gly
275 280 285
Gly Gly Lys Thr Val Ala Leu Glu Glu Val Val Asn Ala Ala Val Glu
290 295 300
Ala Gly Val His Phe Ala Val Ala Ala Gly Asn Asp Asn Ala Asp Ala
305 310 315 320
Cys Asn Tyr Ser Pro Ala Ala Ala Gln Asn Ala Val Thr Val Gly Ala
325 330 335
Ser Ala Leu Asp Asp Ser Arg Ala Tyr Phe Ser Asn Trp Gly Lys Cys
340 345 350
Thr Asp Ile Phe Ala Pro Gly Leu Ser Ile Gln Ser Thr Trp Ile Gly
355 360 365
Ser Lys His Ala Val Asn Thr Ile Ser Gly Thr Ser Met Ala Ser Pro
370 375 380
His Ile Ala Gly Leu Leu Ala Tyr Tyr Leu Ser Leu Gln Pro Ala Ala
385 390 395 400
Asp Ser Glu Tyr Ala Val Ala Ala Ile Thr Pro Ala Glu Leu Lys Ala
405 410 415
Asn Ile Ile Ser Ile Ala Thr Glu Gly Ala Leu Ser Asp Leu Pro Lys
420 425 430
Asp Thr Pro Asn Val Leu Ala Trp Asn Gly Gly Gly Cys Ser Asn Tyr
435 440 445
Ser Ser Ile Leu Glu Gln Gly Ser Tyr Lys Val Lys Ser Ser Lys Asn
450 455 460
Ser Lys Leu Asp Ile Glu Ala Leu Glu Lys Ala Ile Glu Asn Asp Leu
465 470 475 480
Asn Ile Val Ser Gly Gln Ile Val Lys Gly Ala Glu Ser Leu Ala Ser
485 490 495
Lys Ala Gln Lys Phe Ser Thr
500
<210> 111
<211> 532
<212> PRT
<213> 四孢链孢菌
<400> 111
Met Arg Gly Leu Leu Ala Leu Ser Leu Ala Ala Cys Ala Ala Ala Ala
1 5 10 15
Pro Ala Val Ser His Glu Ser Ile His Gly Asp Ala Ala Pro Ile Leu
20 25 30
Ser Ser Ser Asn Ala Glu Val Val Pro Asn Ser Tyr Ile Ile Lys Phe
35 40 45
Lys Lys His Val Thr Asp Asp Lys Ile Ser Asp His His Thr Trp Ile
50 55 60
Gln Lys Ile His Ser Ser Arg Met Asp Leu Lys Lys Arg Ser Gln Ile
65 70 75 80
Pro Met Val Asp Asp Val Phe Arg Gly Leu Lys His Thr Tyr Lys Ile
85 90 95
Gly Gln Asp Phe Met Gly Tyr Ser Gly His Phe Asp Glu Asp Thr Ile
100 105 110
Glu Ala Val Arg Arg His Pro Asp Val Glu Tyr Ile Glu Arg Asp Ser
115 120 125
Ile Val His Thr Met Ser Val Ser Glu Asp Val Asp Ser Glu Gly Lys
130 135 140
Cys Asp Ser Asp Ile Glu Lys Ser Ala Pro Trp Gly Leu Ala Arg Ile
145 150 155 160
Ser His Arg Asp Thr Leu Ser Phe Ala Thr Phe Asn Lys Tyr Leu Tyr
165 170 175
Ala Ala Glu Gly Gly Glu Gly Val Asp Ala Tyr Val Ile Asp Thr Gly
180 185 190
Thr Asn Val Glu His Val Asp Phe Glu Gly Arg Ala Lys Trp Gly Lys
195 200 205
Thr Ile Pro Asn Gly Asp Ala Asp Val Asp Gly Asn Gly His Gly Thr
210 215 220
His Cys Ser Gly Thr Ile Ala Gly Lys Lys Tyr Gly Val Ala Lys Lys
225 230 235 240
Ala Ser Val Tyr Ala Val Lys Val Leu Arg Ser Asn Gly Ser Gly Thr
245 250 255
Met Ala Asp Val Val Ala Gly Val Glu Trp Ala Ala Lys Ser His Leu
260 265 270
Glu Gln Val Lys Ala Ala Lys Asp Gly Lys Arg Lys Gly Phe Lys Gly
275 280 285
Ser Val Ala Asn Met Ser Leu Gly Gly Gly Lys Thr Gln Ala Leu Asp
290 295 300
Asp Thr Val Asn Ala Ala Val Ser Val Gly Ile His Phe Ala Val Ala
305 310 315 320
Ala Gly Asn Asp Asn Ala Asp Ala Cys Asn Tyr Ser Pro Ala Ala Ala
325 330 335
Ala Lys Ala Val Thr Val Gly Ala Ser Ala Ile Asp Asp Ser Arg Ala
340 345 350
Tyr Phe Ser Asn Tyr Gly Lys Cys Thr Asp Ile Phe Ala Pro Gly Leu
355 360 365
Ser Ile Leu Ser Thr Trp Ile Gly Ser Lys Tyr Ala Thr Asn Thr Ile
370 375 380
Ser Gly Thr Ser Met Ala Ser Pro His Ile Cys Gly Leu Leu Ala Tyr
385 390 395 400
Tyr Leu Ser Leu Gln Pro Ser Ser Asp Ser Glu Tyr Ser Leu Ala Thr
405 410 415
Ile Ser Pro Glu Lys Met Lys Ala Asn Leu Leu Lys Ile Ala Thr Val
420 425 430
Gly Ala Leu Ser Asp Met Pro Arg Asp Thr Pro Asn Leu Leu Ala Trp
435 440 445
Asn Gly Gly Gly Cys Ser Asn Tyr Ser Ala Ile Val Asp Ala Gly Ser
450 455 460
Tyr Lys Ala Thr Pro Lys Ala Gln Ser Asp Lys Ile Ser Ser Val Ser
465 470 475 480
Glu Leu Glu Lys Ala Ile Glu His Asp Tyr Glu Val Ile Ser Gly Lys
485 490 495
Val Val Lys Gly Val Ser Ser Leu Ser Asp Lys Ala Glu Lys Leu Ser
500 505 510
Glu Lys Ile His Asp Met Val Glu Glu Glu Leu Lys Glu Phe Leu Glu
515 520 525
Glu Ile Ala His
530
<210> 112
<211> 532
<212> PRT
<213> 嗜热毛壳菌
<400> 112
Met Arg Gly Leu Phe Phe Leu Ser Leu Ala Ala Tyr Ala Ala Ala Ala
1 5 10 15
Pro Arg Ala Lys Val Gly Thr Ile Asp Asn Asp Ala Ala Pro Ile Leu
20 25 30
Ser Ser Ser Asn Ala Glu Ile Val Pro Asn Ala Tyr Ile Ile Lys Phe
35 40 45
Lys Lys His Val Thr Pro Glu Val Ala Ala Ala His His Thr Trp Ile
50 55 60
Gln Glu Ile His Ser Ala Ala Glu Thr Arg Arg Leu Asp Leu Lys Lys
65 70 75 80
Arg Ser Gln Phe Pro Phe Val Asp Gln Val Phe His Gly Leu Lys His
85 90 95
Thr Tyr Gln Ile Gly Gln Glu Phe Leu Gly Tyr Ala Gly His Phe Asp
100 105 110
Asp Glu Thr Ile Glu Gln Val Arg Arg His Pro Asp Val Glu Tyr Ile
115 120 125
Glu Arg Asp Thr Ile Val His Thr Met Arg Phe Glu Glu Ala Lys Glu
130 135 140
Glu Cys His Gly Glu Ile Glu Lys Ser Ala Pro Trp Gly Leu Ala Arg
145 150 155 160
Ile Ser His Arg Asp Thr Leu Thr Phe Ser Thr Phe Asn Lys Tyr Leu
165 170 175
Tyr Ala Ala Glu Gly Gly Glu Gly Val Asp Ala Tyr Val Ile Asp Thr
180 185 190
Gly Thr Asn Ile Glu His Val Asp Phe Glu Gly Arg Ala Lys Trp Gly
195 200 205
Lys Thr Ile Pro Ala Gly Asp Ala Asp Val Asp Gly Asn Gly His Gly
210 215 220
Thr His Cys Ser Gly Thr Ile Ala Gly Lys Lys Tyr Gly Val Ala Lys
225 230 235 240
Lys Ala Asn Ile Tyr Ala Val Lys Val Leu Arg Ser Asn Gly Ser Gly
245 250 255
Thr Met Ser Asp Val Val Ala Gly Val Glu Trp Val Ala Thr Gln His
260 265 270
Leu Glu Asn Val Lys Leu Ala Lys Ala Gly Lys Arg Lys Gly Phe Lys
275 280 285
Gly Ser Val Ala Asn Met Ser Leu Gly Gly Gly Lys Thr Arg Thr Leu
290 295 300
Asp Asp Thr Val Asn Ala Ala Val Ala Val Gly Val His Phe Ala Val
305 310 315 320
Ala Ala Gly Asn Asp Asn Ala Asp Ala Cys Asn Tyr Ser Pro Ala Ala
325 330 335
Ala Glu Ser Ala Ile Thr Val Gly Ala Ser Ala Ile Asp Asp Ser Arg
340 345 350
Ala Tyr Phe Ser Asn Tyr Gly Lys Cys Thr Asp Ile Phe Ala Pro Gly
355 360 365
Leu Ser Ile Leu Ser Thr Trp Ile Gly Ser Lys Tyr Ala Thr Asn Thr
370 375 380
Ile Ser Gly Thr Ser Met Ala Ser Pro His Ile Ala Gly Leu Leu Ala
385 390 395 400
Tyr Tyr Leu Ser Leu Gln Pro Ser Glu Asp Ser Glu Tyr Ser Val Ala
405 410 415
Pro Ile Thr Pro Lys Lys Met Lys Glu Asn Leu Leu Lys Ile Ala Thr
420 425 430
Glu Gly Ala Leu Ser Asp Leu Pro Arg Asp Thr Pro Asn Leu Leu Ala
435 440 445
Trp Asn Gly Gly Gly Cys Ser Asn Tyr Ser Ala Ile Ile Glu Ala Gly
450 455 460
Gly Tyr Lys Val Glu Arg Lys Ser Gln Ser Ser Lys Ile Glu Ile Pro
465 470 475 480
Thr Ser Phe Ser Asp Val Glu Glu Glu Leu Lys Ser Ile Ser Lys Lys
485 490 495
Val His Glu Gly Ala Ser Ser Phe Ala Asp Arg Ala Lys Lys Phe Thr
500 505 510
Glu Lys Ile His Lys Leu Val Asp Glu Glu Leu Lys Glu Phe Ile Glu
515 520 525
Glu Leu Gly Ala
530
<210> 113
<211> 510
<212> PRT
<213> Metarhizium flavoviride
<400> 113
Pro Phe Val Ala Ala Val Ala Leu Ser Val Val Gly Val Ala Gln Ala
1 5 10 15
Gly Phe Ser Ile Glu Thr Ile His Lys Glu Ser Ala Pro Ile Leu Thr
20 25 30
His Val Asp Ala Glu Val Val Pro Asp Ser Tyr Ile Ile Lys Phe Lys
35 40 45
Asp His Val Asn Glu Ala Ala Val Asn Asp His His Ser Trp Ile Gln
50 55 60
Ser Ile His Lys Asp Gly Glu Glu Glu Arg Leu Glu Leu Arg Lys Arg
65 70 75 80
Ser Leu Gly Ser Ser Pro Val Glu Ala Phe Ala Gly Leu Lys His Thr
85 90 95
Tyr Asn Ile Ala Ser Gly Phe Lys Gly Tyr Ser Gly His Phe His Glu
100 105 110
Ser Ile Ile Glu Lys Val Arg Asn His Gly Asp Val Glu Phe Ile Glu
115 120 125
Lys Asp Thr Ile Ala Arg Ile Leu Arg Pro Val Thr Pro Asp Glu Ser
130 135 140
Val Gln Glu Asp Cys Thr Pro Glu Thr Glu Lys Gln Ala Pro Trp Gly
145 150 155 160
Leu Ala Arg Val Ser His Arg Lys Gly Leu Ser Phe Gly Thr Tyr Asn
165 170 175
Lys Tyr Leu Tyr Ala Ala Asp Gly Gly Glu Gly Val Asp Ala Tyr Val
180 185 190
Ile Asp Thr Gly Thr Asn Thr Asp His Val Asp Phe Glu Gly Arg Ala
195 200 205
Lys Trp Gly Lys Thr Ile Pro Ala Gly Asp Ala Asp Glu Asp Gly Asn
210 215 220
Gly His Gly Thr His Cys Ser Gly Thr Ile Ala Gly Lys Lys Tyr Gly
225 230 235 240
Val Ala Lys Lys Ala Asn Val Tyr Ala Val Lys Val Leu Arg Ser Asn
245 250 255
Gly Ser Gly Thr Met Ala Asp Val Val Lys Gly Val Glu Phe Ala Ala
260 265 270
Thr Arg His Val Glu Gln Val Leu Arg Ala Lys Asp Gly Arg Arg Lys
275 280 285
Gly Phe Lys Gly Ser Val Ala Asn Met Ser Leu Gly Gly Gly Lys Thr
290 295 300
Gln Ala Leu Asp Ala Ala Val Asn Ala Ala Val Lys Ala Gly Ile His
305 310 315 320
Phe Ala Val Ala Ala Gly Asn Asp Asn Ala Asp Ala Cys Asn Tyr Ser
325 330 335
Pro Ala Ala Ala Glu Leu Pro Val Thr Val Gly Ala Ser Ala Phe Asp
340 345 350
Asp Ser Arg Ala Tyr Phe Ser Asn Tyr Gly Lys Cys Thr Asp Ile Phe
355 360 365
Ala Pro Gly Leu Asn Ile Leu Ser Thr Trp Ile Gly Ser Pro Thr Ala
370 375 380
Val Asn Thr Ile Ser Gly Thr Ser Met Ala Ser Pro His Ile Cys Gly
385 390 395 400
Leu Leu Ala Tyr Tyr Leu Ser Leu Gln Pro Ala Gly Asp Ser Glu Phe
405 410 415
Ser Val Ala Ser Ile Thr Pro Lys Gln Leu Lys Glu Thr Leu Ile Asp
420 425 430
Ile Ser Thr Gln Gly Val Leu Thr Asp Ile Pro Asn Asp Thr Pro Asn
435 440 445
Lys Leu Ala Trp Asn Gly Gly Gly Cys Ser Asn Tyr Ser Lys Ile Val
450 455 460
Ala Ala Gly Gly Tyr Ile Gly Val Pro Arg Thr Ser Glu Pro Lys Pro
465 470 475 480
Val Gly Asn Ala Val Lys Glu Glu Met Lys Val Leu Ser His Lys Val
485 490 495
Ser Gln Gly Val Lys Asp Leu Gly Asp Lys Thr Glu Lys Gly
500 505 510
<210> 114
<211> 531
<212> PRT
<213> 鹅掌柄孢壳
<400> 114
Met Lys Gly Tyr Ala Ile Leu Ser Leu Ala Ala Ala Ala Ala Ala Ala
1 5 10 15
Pro Ser Ala Ser Ile Glu Thr Ile His Gly Glu Ala Ala Pro Ile Leu
20 25 30
Ser Ser Ala Asn Ala Glu Val Ile Pro Asn Ala Tyr Ile Val Lys Phe
35 40 45
Lys Lys His Val Thr Glu Glu Lys Val Ser Asp His His Thr Trp Ile
50 55 60
Gln Glu Leu His Thr Thr Arg Glu Asn Glu Arg Ile Asp Leu Lys Lys
65 70 75 80
Arg Gly Gln Phe Pro Leu Val Asp Asp Val Phe His Gly Leu Lys His
85 90 95
Thr Tyr Lys Val Gly Ser Glu Phe Leu Gly Tyr Ala Gly His Phe Asp
100 105 110
Glu Glu Thr Ile Glu Lys Val Arg Arg His Pro Asp Val Glu Tyr Ile
115 120 125
Glu Arg Asp Ser Ile Val His Thr Met Arg Val Ile Glu Asp Ala Lys
130 135 140
Cys Asp Ser Glu Ile Glu Lys Ala Ala Pro Trp Gly Leu Ala Arg Ile
145 150 155 160
Ser His Arg Asp Thr Leu Gly Phe Ser Thr Phe Asn Lys Tyr Leu Tyr
165 170 175
Ala Ala Glu Gly Gly Glu Gly Val Asp Ala Tyr Val Ile Asp Thr Gly
180 185 190
Thr Asn Val Asp His Val Asp Phe Asp Gly Arg Ala Lys Trp Gly Lys
195 200 205
Thr Ile Pro Ser Gly Asp Ala Asp Val Asp Gly Asn Gly His Gly Thr
210 215 220
His Cys Ser Gly Thr Ile Ala Gly Lys Lys Tyr Gly Val Ala Lys Lys
225 230 235 240
Ala Asn Val Tyr Ala Val Lys Val Leu Arg Ser Asn Gly Ser Gly Thr
245 250 255
Met Ser Asp Val Val Ala Gly Val Glu Trp Ala Ala Lys Ser His Ile
260 265 270
Gln Ala Val Lys Glu Ala Lys Asp Gly Lys Arg Lys Gly Phe Lys Gly
275 280 285
Ser Val Ala Asn Met Ser Leu Gly Gly Gly Lys Thr Lys Thr Leu Asp
290 295 300
Asp Thr Val Asn Ala Ala Val Ser Val Gly Ile His Phe Ala Val Ala
305 310 315 320
Ala Gly Asn Asp Asn Ala Asp Ala Cys Asn Tyr Ser Pro Ala Ala Ala
325 330 335
Ala Lys Ala Val Thr Val Gly Ala Ser Gly Ile Asp Asp Ser Arg Ala
340 345 350
Tyr Phe Ser Asn Tyr Gly Lys Cys Thr Asp Ile Phe Ala Pro Gly Leu
355 360 365
Ser Ile Leu Ser Thr Trp Ile Gly Ser Lys Tyr Ala Thr Asn Thr Ile
370 375 380
Ser Gly Thr Ser Met Ala Ser Pro His Ile Ala Gly Leu Leu Ala Tyr
385 390 395 400
Tyr Leu Ser Leu Gln Pro Ala Thr Asp Ser Glu Tyr Ser Leu Ala Pro
405 410 415
Ile Thr Pro Glu Lys Leu Lys Asp Asn Leu Leu Lys Ile Ala Thr Glu
420 425 430
Asp Ala Leu Thr Asp Met Pro Lys Gly Thr Pro Asn Leu Leu Ala Trp
435 440 445
Asn Gly Ala Gly Cys Asn Asn Tyr Thr Ala Ile Val Glu Ala Gly Gly
450 455 460
Tyr Lys Val Lys Lys Thr Glu Gly Thr Lys Ala Asp Phe Asp Val Ser
465 470 475 480
Lys Leu Glu Glu Leu Ile Glu Asn Glu Tyr Asp Val Ile Ser Gly Gln
485 490 495
Val Val Lys Gly Val Ser Ser Leu Ser Glu Lys Ala Arg Lys Phe Ser
500 505 510
Lys Lys Ile His Glu Met Val Asp Glu Glu Leu Lys Glu Phe Leu Glu
515 520 525
Glu Leu Ala
530
<210> 115
<211> 536
<212> PRT
<213> 稻瘟病菌
<400> 115
Met Lys Ser Val Ile Leu Leu Ser Leu Ala Ala Cys Ala Val Ala Ala
1 5 10 15
Pro Thr Ala Gly Val Glu Thr Ile His Asp Gly Ala Ala Pro Ile Leu
20 25 30
Ser Ser Ser Asn Ala Glu Ala Ile Pro Asn Ala Tyr Ile Ile Lys Phe
35 40 45
Lys Lys His Val Asp His Lys Ser Ala Ala Asp His Gln Met Trp Ile
50 55 60
Gln Lys Val His Gly Glu Arg Glu Asp Glu Arg Leu Glu Leu Arg Lys
65 70 75 80
Arg Gly Leu Phe Asp Ser Val Asn Asp Ala Phe Thr Gly Leu Lys His
85 90 95
Thr Tyr Asn Val Gly Ser Gly Phe Leu Gly Tyr Ala Gly His Phe Asp
100 105 110
Glu Glu Thr Ile Glu Lys Val Arg Arg His Pro Asp Val Glu Ala Ile
115 120 125
Glu Arg Asp Thr Ile Val His Thr Met Arg Tyr Glu Glu Val Lys Lys
130 135 140
Asp Glu Cys Asn Pro Asp Leu Glu Lys Gly Ala Pro Trp Gly Leu Ser
145 150 155 160
Arg Val Ser His Arg Glu Ser Leu Ser Phe Ser Thr Tyr Asn Lys Tyr
165 170 175
Leu Tyr Ser Ala Glu Gly Gly Glu Gly Val Asp Ala Tyr Val Ile Asp
180 185 190
Thr Gly Thr Asn Ile Asp His Val Asp Phe Glu Gly Arg Ala His Trp
195 200 205
Gly Lys Thr Ile Pro Ala Asn Asp Gln Asp Ile Asp Gly Asn Gly His
210 215 220
Gly Thr His Cys Ser Gly Thr Val Ala Gly Lys Lys Tyr Gly Val Ala
225 230 235 240
Lys Lys Ala Gln Val Tyr Ala Val Lys Val Leu Lys Ser Asn Gly Ser
245 250 255
Gly Thr Met Ser Asp Val Ile Ala Gly Val Asp Phe Ala Ala Lys Ser
260 265 270
His Lys Ala Gln Val Ser Ala Ala Lys Asp Gly Lys Arg Lys Gly Phe
275 280 285
Lys Gly Ser Val Ala Asn Met Ser Leu Gly Gly Gly Lys Thr Thr Leu
290 295 300
Leu Asp Ala Ala Val Asn Ala Ala Val Asp Ala Gly Ile His Phe Ala
305 310 315 320
Val Ala Ala Gly Asn Asp Asn Ala Asp Ala Cys Asn Tyr Ser Pro Ala
325 330 335
Ala Ala Ala Lys Ala Val Thr Val Gly Ala Ser Ala Leu Asp Asp Ser
340 345 350
Arg Ala Tyr Phe Ser Asn Trp Gly Lys Cys Thr Asp Ile Phe Ala Pro
355 360 365
Gly Leu Asn Ile Gln Ser Thr Trp Ile Gly Ser Lys Thr Ala Ile Asn
370 375 380
Thr Ile Ser Gly Thr Ser Met Ala Ser Pro His Ile Ala Gly Leu Leu
385 390 395 400
Ala Tyr Tyr Leu Ser Leu Gln Pro Ala Ser Asp Ser Glu Tyr Ser Leu
405 410 415
Ala Thr Ile Thr Pro Glu Lys Leu Lys Ala Asp Leu Ile Lys Val Gly
420 425 430
Thr Val Gly Ile Leu Thr Asp Ile Pro Lys Asp Thr Pro Asn Val Leu
435 440 445
Ala Trp Asn Gly Gly Gly Cys Ser Asn Tyr Phe Glu Ile Val Ser Lys
450 455 460
Gly Gly Tyr Lys Ala Lys Ala Gln Ala Asp Lys Ser Ser Ser Leu Leu
465 470 475 480
Asp Ser Val Thr Glu Leu Glu Lys Ala Ile Glu His Asp Phe Arg Val
485 490 495
Ile Ser Gly Lys Val Val Lys Glu Ala Ser Ser Met Thr Gly Gln Ala
500 505 510
Glu Lys Leu Ser Glu Lys Ile His Gln Ala Val Asp Glu Glu Leu Lys
515 520 525
His Phe Phe Gly Glu Ala Arg Val
530 535
<210> 116
<211> 522
<212> PRT
<213> 核盘菌
<400> 116
Met Arg Gly Leu Val Ser Leu Ser Leu Leu Pro Leu Leu Ala Ser Ser
1 5 10 15
Ala Pro Thr Phe Ser Thr Glu Thr Ile His Glu Gly Val Ala Pro Leu
20 25 30
Leu Ser Ser Ser Asn Ser Val Ala Val Pro Asp Ser Tyr Ile Val Val
35 40 45
Phe Lys Lys His Val Thr Glu Ala Ser Ala Thr Glu His His Ser Trp
50 55 60
Val Gln Asp Ile His Ser Asn Gly Glu Ala Gln Arg Thr Glu Leu Arg
65 70 75 80
Lys Arg Gly Gln Phe Pro Ile Thr Thr Asp Ile Phe Glu Gly Leu Lys
85 90 95
His Thr Tyr Asn Ile Ala Gly Asp Phe Leu Gly Tyr Ser Gly His Phe
100 105 110
Asp Asp Ser Val Ile Glu Glu Val Arg Arg His Pro Asp Val Glu Tyr
115 120 125
Val Glu Lys Asp Ser Ile Val His Thr Leu Gly Asp Ser Glu Gly Glu
130 135 140
Ile Glu Lys Asn Ala Pro Trp Gly Leu Ala Arg Ile Ser His Arg Asp
145 150 155 160
Ser Leu Ser Phe Gly Thr Phe Asn Lys Tyr Leu Tyr Ala Ala Asp Ala
165 170 175
Gly Glu Gly Val Asp Ala Tyr Val Ile Asp Thr Gly Thr Asn Thr Glu
180 185 190
His Val Asp Phe Glu Gly Arg Ala His Trp Gly Lys Thr Ile Pro Ala
195 200 205
Gly Asp Ala Asp Glu Asp Gly Asn Gly His Gly Thr His Cys Ser Gly
210 215 220
Thr Val Ala Gly Lys Lys Tyr Gly Val Ala Lys Lys Ala Asn Val Tyr
225 230 235 240
Ala Val Lys Val Leu Arg Ser Asn Gly Ser Gly Thr Met Ala Asp Val
245 250 255
Val Lys Gly Val Glu Trp Ala Ala Asn Ser His Thr Lys Lys Val Lys
260 265 270
Glu Gly Lys Lys Gly Phe Lys Gly Ser Val Ala Asn Met Ser Leu Gly
275 280 285
Gly Gly Lys Ser Pro Ala Leu Asp Arg Ala Val Asn Gly Ala Val Ala
290 295 300
Ala Gly Ile His Phe Ala Val Ala Ala Gly Asn Asp Asn Ala Asp Ser
305 310 315 320
Cys Asn Tyr Ser Pro Ala Ala Ala Glu Asn Ala Ile Thr Val Gly Ala
325 330 335
Ser Ala Val Asp Asp Ser Arg Ala Tyr Phe Ser Asn Tyr Gly Thr Cys
340 345 350
Asn Asp Ile Phe Ala Pro Gly Leu Ser Ile Leu Ser Thr Trp Ile Gly
355 360 365
Ser Lys Tyr Ala Val Asn Thr Ile Ser Gly Thr Ser Met Ala Ser Pro
370 375 380
His Ile Cys Gly Leu Leu Ala Tyr Tyr Leu Ser Leu Gln Pro Ser Ser
385 390 395 400
Asp Ser Ala Tyr Ala Val Ala Glu Ile Thr Pro Lys Lys Met Lys Glu
405 410 415
Asn Leu Leu Ser Val Ala Thr Glu Gly Ala Leu Ser Asp Val Pro Ser
420 425 430
Asn Thr Lys Asn Ile Leu Ala Trp Asn Gly Gly Gly Ser Ser Asn Tyr
435 440 445
Thr Ser Ile Ile Lys Ala Gly Gly Tyr Ala Ala Lys Ala Ser Lys Lys
450 455 460
Val Pro Thr Thr Val Glu Leu Leu Glu Lys Ala Ile Glu Asn Asp Phe
465 470 475 480
Asn Met Leu Ser Gly Lys Val Val Lys Gly Gly Glu Ser Ile Val Ser
485 490 495
Lys Thr Glu Glu Leu Ser Lys Lys Ile Gln Lys Ile Val Asp Glu Glu
500 505 510
Ile Ser Glu Phe Phe Glu Glu Phe Ser Ser
515 520
<210> 117
<211> 491
<212> PRT
<213> 柄篮状菌
<400> 117
Met Lys Gly Ile Leu Gly Leu Ser Leu Leu Pro Leu Leu Thr Val Ala
1 5 10 15
Ser Pro Val Met Pro Ser Thr Ile His Asn Asp Ala Ala Pro Ile Leu
20 25 30
Ser Ser Ser Asn Ala Val Glu Val Pro Asp Ser Tyr Ile Ile Val Phe
35 40 45
Lys Asn His Val Asp Pro Ala Ser Ala Ala Ala His His Ser Trp Val
50 55 60
Gln Asp Ile His Asn Gln His Thr Glu Leu Arg Lys Arg Ser Gln Phe
65 70 75 80
Pro Phe Ala Gly Asp Val Phe Ala Gly Leu Lys His Thr Phe Asp Ile
85 90 95
Ala Gly Ser Phe Leu Gly Tyr Ser Gly His Phe Asp Glu Asn Val Ile
100 105 110
Glu Ala Ile Arg Arg His Pro Asp Val Asp Tyr Ile Glu Lys Asp Ser
115 120 125
Ile Val His Thr Met Glu Asp Pro Thr Thr Glu Lys Asn Ala Pro Trp
130 135 140
Gly Leu Ala Arg Ile Ser His Arg Glu Ser Leu Ser Phe Gly Ser Phe
145 150 155 160
Asn Lys Tyr Leu Tyr Ala Ala Asp Gly Gly Glu Gly Val Asp Val Tyr
165 170 175
Val Ile Asp Thr Gly Thr Asn Ile Asp His Val Asp Phe Glu Gly Arg
180 185 190
Ala Ser Trp Gly Lys Thr Ile Pro Thr Asp Asp Glu Asp Val Asp Gly
195 200 205
Asn Gly His Gly Thr His Cys Ser Gly Thr Ile Ala Gly Lys Lys Tyr
210 215 220
Gly Val Ala Lys Lys Ala Asn Val Tyr Ala Val Lys Val Leu Lys Ser
225 230 235 240
Asn Gly Ser Gly Thr Met Ser Asp Val Val Gln Gly Val Glu Trp Ala
245 250 255
Ala Thr Gln His Ile Lys Lys Val Lys Asp Ala Lys Ala Gly Lys Ala
260 265 270
Lys Gly Phe Lys Gly Ser Ala Ala Asn Met Ser Leu Gly Gly Gly Lys
275 280 285
Ser Val Thr Leu Asp Arg Ala Val Asn Ala Ala Val Asp Ala Gly Ile
290 295 300
His Phe Ala Val Ala Ala Gly Asn Asp Asn Ala Asp Ser Cys Asn Tyr
305 310 315 320
Ser Pro Ala Ala Ala Glu Lys Ala Val Thr Val Gly Ala Ser Thr Leu
325 330 335
Ala Asp Glu Arg Ala Tyr Phe Ser Asn Tyr Gly Lys Cys Asn Asp Ile
340 345 350
Phe Ala Pro Gly Leu Asn Ile Leu Ser Thr Trp Ile Gly Ser Lys Tyr
355 360 365
Ala Val Asn Thr Ile Ser Gly Thr Ser Met Ala Ser Pro His Ile Ala
370 375 380
Gly Leu Leu Ala Tyr Phe Leu Ser Leu Gln Pro Ala Ser Asp Ser Ala
385 390 395 400
Phe Ala Val Ala Glu Ile Thr Pro Lys Lys Leu Lys Glu Asn Leu Ile
405 410 415
Thr Ile Gly Thr Gln Gly Val Leu Thr Asp Val Pro Ser Asp Thr Thr
420 425 430
Asn Ile Leu Ala Trp Asn Gly Gly Gly Ser Ser Asn Tyr Ser Asp Ile
435 440 445
Ile Ala Lys Gly Gly Tyr Lys Thr Lys Ser Val Ser Asp Gln Val Asp
450 455 460
Asp Leu Ile Ile Lys Ala Gln Glu Val Val Asn Glu Glu Leu Gly Ala
465 470 475 480
Ile Tyr Ser His Ile Lys Asp Ala Val Val Ala
485 490
<210> 118
<211> 256
<212> PRT
<213> 里氏木霉
<400> 118
Met Phe Ile Ala Gly Val Ala Leu Ser Ala Leu Leu Cys Ala Asp Thr
1 5 10 15
Val Leu Ala Gly Val Ala Gln Asp Arg Gly Leu Ala Ala Arg Leu Ala
20 25 30
Arg Arg Ala Gly Arg Arg Ser Ala Pro Phe Arg Asn Asp Thr Ser His
35 40 45
Ala Thr Val Gln Ser Asn Trp Gly Gly Ala Ile Leu Glu Gly Ser Gly
50 55 60
Phe Thr Ala Ala Ser Ala Thr Val Asn Val Pro Arg Gly Gly Gly Gly
65 70 75 80
Ser Asn Ala Ala Gly Ser Ala Trp Val Gly Ile Asp Gly Ala Ser Cys
85 90 95
Gln Thr Ala Ile Leu Gln Thr Gly Phe Asp Trp Tyr Gly Asp Gly Thr
100 105 110
Tyr Asp Ala Trp Tyr Glu Trp Tyr Pro Glu Phe Ala Ala Asp Phe Ser
115 120 125
Gly Ile Asp Ile Arg Gln Gly Asp Gln Ile Ala Met Ser Val Val Ala
130 135 140
Thr Ser Leu Thr Gly Gly Ser Ala Thr Leu Glu Asn Leu Ser Thr Gly
145 150 155 160
Gln Lys Val Thr Gln Asn Phe Asn Arg Val Thr Ala Gly Ser Leu Cys
165 170 175
Glu Thr Ser Ala Glu Phe Ile Ile Glu Asp Phe Glu Glu Cys Asn Ser
180 185 190
Asn Gly Ser Asn Cys Gln Pro Val Pro Phe Ala Ser Phe Ser Pro Ala
195 200 205
Ile Thr Phe Ser Ser Ala Thr Ala Thr Arg Ser Gly Arg Ser Val Ser
210 215 220
Leu Ser Gly Ala Glu Ile Thr Glu Val Ile Val Asn Asn Gln Asp Leu
225 230 235 240
Thr Arg Cys Ser Val Ser Gly Ser Ser Thr Leu Thr Cys Ser Tyr Val
245 250 255
<210> 119
<211> 256
<212> PRT
<213> 深绿木霉
<400> 119
Met Lys Phe Ile Thr Gly Ile Ala Ile Ser Ala Leu Leu Cys Ala Asp
1 5 10 15
Ala Ala Leu Ala Gly Val Ala Ala Asn Arg Gly Leu Ala Arg Arg Leu
20 25 30
Ala Arg Arg Ala Gly Arg Arg Ser Ala Pro Phe Lys Asn Asn Thr Leu
35 40 45
His Ala Ser Val Gln Ser Asn Trp Gly Gly Ala Ile Leu Gln Gly Ser
50 55 60
Gly Tyr Ser Ala Ala Ser Ala Thr Val Asn Val Pro Thr Gly Gly Gly
65 70 75 80
Gly Ser Ser Ala Ala Gly Thr Ala Trp Val Gly Ile Asp Gly Asp Thr
85 90 95
Cys Asp Ser Ala Ile Leu Gln Thr Gly Ile Asp Trp Tyr Gly Asp Gly
100 105 110
Thr Tyr Asp Ala Trp Tyr Glu Trp Tyr Pro Glu Phe Ser Ala Asp Phe
115 120 125
Ser Gly Ile Asp Ile Ser Ala Gly Asp Gln Ile Ala Met Ser Val Val
130 135 140
Ala Thr Ser Leu Thr Gly Gly Ser Ala Thr Leu Glu Asn Leu Thr Ser
145 150 155 160
Gly Gln Lys Val Thr Gln Asn Phe Ser Lys Val Thr Ala Gly Ser Leu
165 170 175
Cys Glu Thr Ser Ala Glu Phe Ile Ile Glu Asp Phe Glu Glu Cys Asn
180 185 190
Glu Asn Gly Ser Asp Cys Gln Phe Val Pro Phe Ala Ser Phe Ser Pro
195 200 205
Ala Val Lys Phe Ser Ser Ala Ser Ala Thr Gln Asn Gly Arg Thr Val
210 215 220
Ser Leu Ser Gly Ala Val Leu Thr Glu Val Glu Ile Asn Asn Lys Asp
225 230 235 240
Val Thr Lys Cys Ser Val Ser Gly Ser Thr Leu Thr Cys Ser Tyr Val
245 250 255
<210> 120
<211> 256
<212> PRT
<213> 绿木霉
<400> 120
Met Met Phe Val Ala Gly Val Ala Leu Thr Ala Leu Leu Cys Ala Asp
1 5 10 15
Ala Ala Leu Ala Gly Val Ala Gln Asn Arg Gly Leu Ala Ala Arg Leu
20 25 30
Ala Arg Arg Ala Gly Arg Arg Ser Ala Pro Met Arg Asn Asn Thr Ser
35 40 45
His Ala Thr Val Gln Ser Asn Trp Gly Gly Ala Ile Leu Glu Gly Ser
50 55 60
Gly Phe Thr Ala Ala Ser Ala Thr Val Asn Val Pro Lys Gly Gly Gly
65 70 75 80
Gly Ser Ser Ala Ala Gly Ser Ala Trp Val Gly Ile Asp Gly Ala Thr
85 90 95
Cys Asp Ser Ala Ile Leu Gln Thr Gly Phe Asp Trp Tyr Gly Asp Gly
100 105 110
Thr Tyr Asp Ala Trp Tyr Glu Trp Tyr Pro Glu Phe Ala Ala Asp Phe
115 120 125
Thr Gly Ile Asp Ile Asn Gln Gly Asp Gln Ile Ala Met Ser Val Val
130 135 140
Ala Thr Ser Leu Thr Gly Gly Ser Ala Thr Leu Gln Asn Leu Ser Thr
145 150 155 160
Gly Gln Lys Val Thr Gln Thr Phe Ser Arg Val Thr Ala Gly Ser Leu
165 170 175
Cys Glu Thr Ser Ala Glu Phe Ile Ile Glu Asp Phe Glu Glu Cys Asn
180 185 190
Ser Asn Gly Ser Asn Cys Gln Pro Val Pro Phe Ala Ser Phe Ser Pro
195 200 205
Ala Ile Gln Phe Thr Ser Ala Ser Ala Thr Arg Ser Gly Gln Lys Val
210 215 220
Ser Leu Ser Gly Ala Val Met Ser Glu Val Ile Val Asn Asn Lys Asp
225 230 235 240
Leu Thr Lys Cys Ser Val Ser Gly Ser Thr Leu Thr Cys Ser Tyr Val
245 250 255
<210> 121
<211> 271
<212> PRT
<213> 黄曲霉
<400> 121
Met Lys Leu Phe Ala Ile Leu Ser Ser Thr Val Leu Ala Ser Val Ala
1 5 10 15
Leu Ala Ser Pro Leu Thr Leu Glu Arg Arg Ala Arg Asn Ala Ala Arg
20 25 30
Leu Gln Ala Arg Val Ala Gln Arg His Ser Asn Leu Pro Phe Lys Ala
35 40 45
Gly Thr Asn Glu Ile Leu His Leu Asn Glu Thr Thr His Glu Glu Tyr
50 55 60
Ser Ser Asn Trp Ala Gly Ala Val Leu Ile Gly Ser Gly Tyr Thr Ser
65 70 75 80
Val Thr Gly Thr Phe Thr Val Pro Thr Pro Ser Ala Pro Ser Gly Ala
85 90 95
Asp Ser Asp Glu Gln Tyr Cys Ala Thr Ala Trp Val Gly Ile Asp Gly
100 105 110
Asp Thr Cys Gln Ser Ala Ile Leu Gln Thr Gly Val Asp Phe Cys Ile
115 120 125
Asp Ser Ser Gly Thr Thr Phe Asp Ala Trp Tyr Glu Trp Phe Pro Asp
130 135 140
Tyr Ser His Asp Phe Ser Gly Ile Ser Ile Ser Ala Gly Asp Lys Val
145 150 155 160
Lys Val Thr Val Asp Ala Ser Ser Lys Thr Ala Gly Thr Ala Ile Val
165 170 175
Glu Asn Leu Thr Thr Gly Lys Thr Val Ser His Thr Phe Thr Gly Gln
180 185 190
Asp Asp Asn Ala Leu Cys Glu Thr Asn Ala Glu Trp Ile Met Glu Asp
195 200 205
Phe Ser Ser Phe Leu Ser Leu Val Pro Phe Ala Asn Phe Gly Thr Val
210 215 220
Thr Phe Thr Asp Ile Ser Ala Thr Ser Gly Asp Ser Ser Val Gly Ala
225 230 235 240
Ser Asn Ala Thr Ile Ile Asp Ile Gln Gln Asn Asn Lys Thr Leu Thr
245 250 255
Ser Ser Ser Ala Ser Asp Thr Glu Val Thr Ile Lys Tyr Ile Gly
260 265 270
<210> 122
<211> 265
<212> PRT
<213> 黑曲霉
<400> 122
Met Lys Thr Thr Ala Leu Leu Thr Ala Gly Leu Leu Ala Thr Thr Ala
1 5 10 15
Met Ala Ala Pro Leu Thr Ala Lys Arg Gln Ala Ala Arg Ala Lys Arg
20 25 30
Ser Thr Asn Arg Gln Ser Asn Pro Pro Phe Lys Pro Gly Thr Asn Glu
35 40 45
Val Leu Ala Leu Asn Gly Thr Lys Asn Val Glu Tyr Ser Ser Asn Trp
50 55 60
Ala Gly Ala Val Leu Ile Gly Thr Gly Tyr Thr Ala Val Thr Ala Glu
65 70 75 80
Phe Val Val Pro Thr Pro Ser Val Pro Ser Gly Gly Ser Ser Arg Glu
85 90 95
Glu Tyr Cys Ala Ser Ala Trp Val Gly Ile Asp Gly Asp Thr Cys Asp
100 105 110
Thr Ala Ile Leu Gln Thr Gly Val Asp Phe Cys Val Gln Gly Ser Glu
115 120 125
Val Ser Phe Asp Ala Trp Tyr Glu Trp Tyr Pro Asp Tyr Ala Tyr Asp
130 135 140
Phe Ser Gly Ile Ser Ile Ser Ala Gly Asp Thr Ile Lys Val Thr Val
145 150 155 160
Asp Ala Ser Ser Asp Thr Thr Gly Thr Ala Thr Ile Glu Asn Val Ser
165 170 175
Thr Gly Thr Thr Val Thr His Ser Phe Thr Gly Gly Val Asp Gly Asp
180 185 190
Leu Cys Glu Tyr Asn Ala Glu Trp Ile Val Glu Asp Phe Glu Glu Asp
195 200 205
Asp Ser Leu Val Pro Phe Ala Asp Phe Gly Thr Val Thr Phe Thr Ser
210 215 220
Cys Ser Ala Thr Lys Asp Gly Ser Ser Val Gly Pro Glu Asp Ala Thr
225 230 235 240
Ile Ile Asp Ile Glu Gln Asn Glu Val Leu Thr Ser Val Ser Val Ser
245 250 255
Ser Ser Glu Val Val Val Lys Tyr Val
260 265
<210> 123
<211> 268
<212> PRT
<213> 土曲霉
<400> 123
Met Lys Leu Ser Val Leu Ser Ser Ile Leu Leu Ala Gly Thr Ala Val
1 5 10 15
Ala Ala Pro Leu Thr Ala Gln Arg Gln Ala Arg Asn Ala Arg Arg Val
20 25 30
Ala Arg Ala Ala Asn Arg Ser Ser His Pro Pro Tyr Lys Pro Asp Ser
35 40 45
Ser Glu Leu Leu His Leu Ser Asn Thr Thr His Val Glu Tyr Ser Ser
50 55 60
Asn Trp Ala Gly Ala Val Leu Ile Gly Thr Gly Tyr Thr Ala Val Thr
65 70 75 80
Gly Glu Phe Thr Val Pro Thr Pro Ser Thr Pro Ser Gly Ser Ser Ser
85 90 95
Ser Lys Glu Tyr Cys Ala Ser Ala Trp Val Gly Ile Asp Gly Asp Thr
100 105 110
Cys Ser Thr Ala Ile Leu Gln Thr Gly Val Asp Phe Cys Val Gln Gly
115 120 125
Ser Ser Val Ser Phe Asp Ala Trp Tyr Glu Trp Tyr Pro Asp Tyr Ala
130 135 140
Tyr Asp Phe Ser Gly Ile Thr Ile Ser Ala Gly Asp Thr Ile Arg Val
145 150 155 160
Thr Val Asp Ala Thr Ser Lys Thr Ala Gly Thr Ala Thr Val Glu Asn
165 170 175
Leu Thr Thr Gly Lys Thr Val Thr His Thr Phe Thr Gly Gly Val Asp
180 185 190
Gly Ser Leu Cys Glu Tyr Asn Ala Glu Trp Ile Val Glu Asp Phe Glu
195 200 205
Glu Asn Gly Ser Leu Val Pro Phe Ala Asp Phe Gly Thr Val Thr Phe
210 215 220
Ser Ser Ala Glu Ala Thr Asp Gly Gly Ser Thr Val Gly Pro Ser Gly
225 230 235 240
Ala Thr Val Ile Asp Ile Glu Gln Ser Asn Lys Val Leu Thr Ser Val
245 250 255
Ser Val Gly Ser Ser Ser Val Thr Val Lys Tyr Val
260 265
<210> 124
<211> 260
<212> PRT
<213> 木栖柱孢霉
<400> 124
Met Lys Phe Thr Thr Ala Ala Val Leu Ser Ala Leu Val Ser Ala Glu
1 5 10 15
Ile Ala Phe Ala Ala Pro Gly Gly Asn Gly Phe Ala Arg Arg Gln Ala
20 25 30
Arg Arg Gln Ala Arg Ala Ala Gly Leu Lys Ala Ser Pro Phe Arg Gln
35 40 45
Val Asn Ala Lys Glu Ala Thr Val Glu Ser Asn Trp Gly Gly Ala Ile
50 55 60
Leu Ile Gly Ser Asp Phe Asp Thr Val Ser Ala Thr Ala Asn Val Pro
65 70 75 80
Ser Ala Ser Gly Gly Ser Ser Ala Ala Gly Thr Ala Trp Val Gly Ile
85 90 95
Asp Gly Asp Thr Cys Gln Thr Ala Ile Leu Gln Thr Gly Phe Asp Trp
100 105 110
Tyr Gly Asp Gly Thr Tyr Asp Ala Trp Tyr Glu Trp Tyr Pro Glu Val
115 120 125
Ser Asp Asp Phe Ser Gly Ile Thr Ile Ser Glu Gly Asp Ser Ile Gln
130 135 140
Met Ser Val Thr Ala Thr Ser Asp Thr Ser Gly Ser Ala Thr Leu Glu
145 150 155 160
Asn Leu Thr Thr Gly Gln Lys Val Ser Lys Ser Phe Ser Asn Glu Ser
165 170 175
Ser Gly Ser Leu Cys Arg Thr Asn Ala Glu Phe Ile Ile Glu Asp Phe
180 185 190
Glu Glu Cys Asn Ser Asn Gly Ser Asp Cys Glu Phe Val Pro Phe Ala
195 200 205
Ser Phe Ser Pro Ala Val Glu Phe Thr Asp Cys Ser Val Thr Ser Asp
210 215 220
Gly Glu Ser Val Ser Leu Asp Asp Ala Gln Ile Thr Gln Val Ile Ile
225 230 235 240
Asn Asn Gln Asp Val Thr Asp Cys Ser Val Ser Gly Thr Thr Val Ser
245 250 255
Cys Ser Tyr Val
260
<210> 125
<211> 206
<212> PRT
<213> 木栖柱孢霉
<400> 125
Thr Val Glu Ser Asn Trp Gly Gly Ala Ile Leu Ile Gly Ser Asp Phe
1 5 10 15
Asp Thr Val Ser Ala Thr Ala Asn Val Pro Ser Ala Ser Gly Gly Ser
20 25 30
Ser Ala Ala Gly Thr Ala Trp Val Gly Ile Asp Gly Asp Thr Cys Gln
35 40 45
Thr Ala Ile Leu Gln Thr Gly Phe Asp Trp Tyr Gly Asp Gly Thr Tyr
50 55 60
Asp Ala Trp Tyr Glu Trp Tyr Pro Glu Val Ser Asp Asp Phe Ser Gly
65 70 75 80
Ile Thr Ile Ser Glu Gly Asp Ser Ile Gln Met Ser Val Thr Ala Thr
85 90 95
Ser Asp Thr Ser Gly Ser Ala Thr Leu Glu Asn Leu Thr Thr Gly Gln
100 105 110
Lys Val Ser Lys Ser Phe Ser Asn Glu Ser Ser Gly Ser Leu Cys Arg
115 120 125
Thr Asn Ala Glu Phe Ile Ile Glu Asp Phe Glu Glu Cys Asn Ser Asn
130 135 140
Gly Ser Asp Cys Glu Phe Val Pro Phe Ala Ser Phe Ser Pro Ala Val
145 150 155 160
Glu Phe Thr Asp Cys Ser Val Thr Ser Asp Gly Glu Ser Val Ser Leu
165 170 175
Asp Asp Ala Gln Ile Thr Gln Val Ile Ile Asn Asn Gln Asp Val Thr
180 185 190
Asp Cys Ser Val Ser Gly Thr Thr Val Ser Cys Ser Tyr Val
195 200 205
<210> 126
<211> 204
<212> PRT
<213> 木栖柱孢霉
<400> 126
Thr Val Glu Ser Asn Trp Gly Gly Ala Ile Leu Ile Gly Ser Asp Phe
1 5 10 15
Asp Thr Val Ser Ala Thr Ala Asn Val Pro Ser Ala Thr Gly Ala Ser
20 25 30
Gly Gly Ser Ser Ala Ala Trp Val Gly Ile Asp Gly Asp Thr Cys Gln
35 40 45
Thr Ala Ile Leu Gln Thr Gly Phe Asp Trp Tyr Gly Asp Gly Thr Tyr
50 55 60
Asp Ala Trp Tyr Glu Trp Tyr Pro Glu Val Ser Asp Asp Phe Ser Gly
65 70 75 80
Ile Thr Ile Ser Glu Gly Asp Ser Ile Gln Met Ser Val Thr Ala Thr
85 90 95
Ser Asp Thr Ser Gly Ser Ala Thr Leu Glu Asn Leu Thr Thr Gly Gln
100 105 110
Lys Val Ser Lys Ser Phe Ser Asn Glu Ser Ser Gly Leu Cys Arg Thr
115 120 125
Asn Ala Glu Phe Ile Ile Glu Asp Phe Glu Glu Cys Asn Ser Asp Gly
130 135 140
Ser Asp Glu Phe Val Pro Phe Ala Ser Phe Ser Pro Ala Val Glu Phe
145 150 155 160
Thr Asp Cys Ser Val Thr Ser Asp Gly Glu Ser Val Ser Leu Asp Asp
165 170 175
Ala Gln Ile Thr Gln Val Ile Ile Asn Asn Gln Asp Val Thr Asp Cys
180 185 190
Ser Val Ser Gly Thr Thr Val Ser Cys Ser Tyr Val
195 200
<210> 127
<211> 245
<212> PRT
<213> 太瑞斯梭孢壳霉
<400> 127
Met Lys Asn Ala Ile Leu Ile Ala Ala Ala Val Gly Leu Ala Ala Ala
1 5 10 15
Leu Pro Gln Pro Gly Ala Pro Leu Thr Arg Arg Gly Gly Thr Arg Pro
20 25 30
Pro His His Ser Ala Pro Arg Arg Asn Thr Thr Tyr Pro Glu Tyr Ser
35 40 45
Ser Asn Trp Ala Gly Ala Val Gln Ile Gly Thr Gly Phe Thr Phe Val
50 55 60
Thr Gly Thr Ile Thr Val Pro Ser Val Tyr Asn Gly Asn Pro Tyr Ser
65 70 75 80
Ala Ala Ser Ala Trp Val Gly Ile Asp Gly Asp Thr Cys Gln Thr Ala
85 90 95
Ile Leu Gln Thr Gly Val Ser Phe Tyr Gly Asp Gly Ser Tyr Asp Ala
100 105 110
Trp Tyr Glu Trp Ile Pro Asp Tyr Ser Tyr Ser Phe Ser Gly Phe Gly
115 120 125
Leu Arg Ala Gly Asp Glu Ile Arg Met Thr Val Asp Ala Thr Ser Lys
130 135 140
Thr Thr Gly Val Ala Thr Leu Glu Asn Leu Ser Thr Gly Ala Lys Val
145 150 155 160
Ser His Thr Phe Arg Asn Thr Pro Ser Thr Leu Cys Glu Thr Asn Ala
165 170 175
Glu Trp Ile Val Glu Asp Phe Glu Ser Gly Gly Ser Leu Val Pro Phe
180 185 190
Ala Asp Phe Gly Thr Val Thr Phe Thr Asp Ala Tyr Ala Thr Gly Ser
195 200 205
Gly Gly Gln Val Thr Pro Gln Gly Ala Thr Ile Ile Asp Ile Arg Gln
210 215 220
Ser Gly Arg Val Leu Thr Asn Cys Gly Thr Ser Gly Ala Asp Leu Thr
225 230 235 240
Cys Thr Tyr Thr Gly
245
<210> 128
<211> 270
<212> PRT
<213> 产黄青霉
<400> 128
Met Lys Phe Ser Thr Thr Ile Leu Ser Ala Ala Leu Leu Thr Ala Ser
1 5 10 15
Ala Ile Ala Ala Pro Leu Thr Glu Arg Arg Ala Ala Arg His Ala Ala
20 25 30
Arg Ala Ala Asp Arg Ala Gly Arg Ile Ser Arg Pro Ala Ala Lys Gln
35 40 45
Asp Thr Leu Glu Leu Val Ser Leu Asn Gly Thr Ser His Ala Gln Tyr
50 55 60
Ser Ser Asn Trp Ala Gly Ala Val Leu Ile Gly Thr Gly Tyr Thr Ser
65 70 75 80
Val Thr Gly Glu Phe Thr Val Pro Lys Pro Asn Leu Pro Ser Asp Ala
85 90 95
Ser Ser Gly Gln Gln Ser Cys Ala Ser Ala Trp Val Gly Ile Asp Gly
100 105 110
Asp Thr Cys Ser Ser Ala Ile Leu Gln Thr Gly Ile Asp Phe Cys Ile
115 120 125
Gln Gly Gly Glu Val Ser Tyr Asp Ala Trp Tyr Glu Trp Tyr Pro Asp
130 135 140
Tyr Ala His Asp Phe Ser Gly Ile Asp Ile Ser Thr Gly Asp Val Ile
145 150 155 160
Lys Leu Thr Val Thr Ala Thr Ser Lys Ser Ser Gly Ser Ala Val Ile
165 170 175
Asp Asn Val Thr Thr Gly Lys Ser Val Thr His Lys Phe Ser Gly Val
180 185 190
Asp Asp Gly Asp Leu Cys Glu Thr Asn Ala Glu Trp Ile Val Glu Asp
195 200 205
Phe Glu Ser Gly Gly Gln Leu Val Pro Phe Thr Asn Phe Gly Thr Val
210 215 220
Thr Phe Thr Lys Ala Glu Ala Thr Ser Gly Gly Lys Ser Val Gly Pro
225 230 235 240
Ser Gly Ala Thr Leu Phe Glu Ile Arg Gln Ser Lys Lys Val Leu Thr
245 250 255
Lys Ser Ser Val Thr Gly Asn Ser Val Thr Val Ser Tyr Val
260 265 270
<210> 129
<211> 236
<212> PRT
<213> 里氏木霉
<400> 129
Met Asp Ala Ile Arg Ala Arg Ser Ala Ala Arg Arg Ser Asn Arg Phe
1 5 10 15
Gln Ala Gly Ser Ser Lys Asn Val Asn Gly Thr Ala Asp Val Glu Ser
20 25 30
Thr Asn Trp Ala Gly Ala Ala Ile Thr Thr Ser Gly Val Thr Glu Val
35 40 45
Ser Gly Thr Phe Thr Val Pro Arg Pro Ser Val Pro Ala Gly Gly Ser
50 55 60
Ser Arg Glu Glu Tyr Cys Gly Ala Ala Trp Val Gly Ile Asp Gly Tyr
65 70 75 80
Ser Asp Ala Asp Leu Ile Gln Thr Gly Val Leu Trp Cys Val Glu Asp
85 90 95
Gly Glu Tyr Leu Tyr Glu Ala Trp Tyr Glu Tyr Leu Pro Ala Ala Leu
100 105 110
Val Glu Tyr Ser Gly Ile Ser Val Thr Ala Gly Ser Val Val Thr Val
115 120 125
Thr Ala Thr Lys Thr Gly Thr Asn Ser Gly Val Thr Thr Leu Thr Ser
130 135 140
Gly Gly Lys Thr Val Ser His Thr Phe Ser Arg Gln Asn Ser Pro Leu
145 150 155 160
Pro Gly Thr Ser Ala Glu Trp Ile Val Glu Asp Phe Thr Ser Gly Ser
165 170 175
Ser Leu Val Pro Phe Ala Asp Phe Gly Ser Val Thr Phe Thr Gly Ala
180 185 190
Thr Ala Val Val Asn Gly Ala Thr Val Thr Ala Gly Gly Asp Ser Pro
195 200 205
Val Ile Ile Asp Leu Glu Asp Ser Arg Gly Asp Ile Leu Thr Ser Thr
210 215 220
Thr Val Ser Gly Ser Thr Val Thr Val Glu Tyr Glu
225 230 235
<210> 130
<211> 265
<212> PRT
<213> 深绿木霉
<400> 130
Met Lys Ser Leu Thr Leu Ala Ala Asn Leu Leu Leu Val Ala Ser Ala
1 5 10 15
Ala Met Ala Thr Pro Leu Ala Ala Arg Lys Gln Ala Ala Met Asp Ala
20 25 30
Leu Arg Ala Arg Asn Ala Ala Arg Arg Ser Asn Lys Phe Gln Ala Ser
35 40 45
Ser Ser Thr His Val Asn Ser Thr Ala Asp Val Glu Ser Thr Asn Trp
50 55 60
Ala Gly Ala Ala Leu Thr Thr Ser Gly Ile Thr Glu Val Thr Gly Thr
65 70 75 80
Phe Thr Val Pro Lys Pro Thr Val Pro Ser Gly Gly Ser Ser Ser Thr
85 90 95
Glu Tyr Cys Gly Ala Ala Trp Val Gly Ile Asp Gly Tyr Ser Asp Ala
100 105 110
Asp Leu Ile Gln Thr Gly Val Leu Trp Cys Val Gln Ser Gly Glu Tyr
115 120 125
Leu Tyr Glu Ala Trp Tyr Glu Tyr Leu Pro Ala Ser Leu Ile Ala Tyr
130 135 140
Ser Gly Ile Thr Val Thr Ala Gly Ser Val Val Thr Val Thr Ala Thr
145 150 155 160
Lys Thr Gly Asn Asn Ser Gly Thr Thr Thr Leu Thr Ser Gly Gly Lys
165 170 175
Thr Val Ser His Thr Phe Ser Asn Gln Asn Ser Pro Leu Pro Gly Thr
180 185 190
Ser Ala Glu Trp Ile Val Glu Asp Phe Thr Ser Gly Ser Ser Leu Val
195 200 205
Pro Phe Ala Asp Phe Gly Ser Val Thr Phe Thr Gly Ser Ser Ala Leu
210 215 220
Val Asn Gly Gln Thr Val Thr Pro Gly Ser Gly Ser Ser Thr Ile Ile
225 230 235 240
Asp Leu Glu Asn Ser Arg Gly Gln Ile Ile Thr Ser Thr Ser Ile Ser
245 250 255
Gly Gly Ser Val Thr Val Asn Tyr Glu
260 265
<210> 131
<211> 237
<212> PRT
<213> 绿木霉
<400> 131
Met Asp Ala Ile Arg Ala Arg Asn Ala Val Arg Arg Ser Asn Arg Phe
1 5 10 15
Leu Ala Gly Ser Asn Ser Asn Asn Val Asn Gly Thr Ala Asp Val Glu
20 25 30
Ser His Asn Trp Ala Gly Ala Ala Leu Thr Thr Ser Gly Val Thr Glu
35 40 45
Val Ser Gly Thr Phe Thr Val Pro Lys Pro Ser Ile Pro Ala Gly Gly
50 55 60
Ser Ser Arg Thr Glu Tyr Cys Gly Ala Ala Trp Val Gly Ile Asp Gly
65 70 75 80
Tyr Ser Asn Ala Asp Leu Ile Gln Thr Gly Val Leu Trp Cys Val Glu
85 90 95
Gly Gly Gln Phe Leu Tyr Glu Ala Trp Tyr Glu Tyr Leu Pro Ala Ser
100 105 110
Leu Val Ala Tyr Ser Gly Ile Ser Val Thr Ala Gly Ser Val Val Thr
115 120 125
Val Thr Ala Thr Lys Thr Gly Thr Asn Ser Gly Val Thr Thr Leu Thr
130 135 140
Ser Gly Gly Lys Thr Val Ser His Thr Phe Ser Arg Glu Ser Ser Ser
145 150 155 160
Leu Pro Gly Thr Ser Ala Glu Trp Ile Val Glu Asp Phe Thr Ser Gly
165 170 175
Ser Ser Leu Val Pro Phe Ala Asn Phe Gly Ser Val Thr Phe Thr Gly
180 185 190
Ala Ser Ala Val Val Asn Gly Ala Thr Val Thr Pro Gly Ser Gly Ser
195 200 205
Ala Val Ile Ile Asp Leu Glu Asp Ala Ser Gly Asp Ile Ile Thr Ser
210 215 220
Thr Ser Val Ser Gly Ser Ser Leu Thr Val Asn Tyr Glu
225 230 235
<210> 132
<211> 259
<212> PRT
<213> 家蚕
<400> 132
Met Lys Phe Thr Ser Leu Thr Ala Asn Leu Leu Leu Val Ala Thr Thr
1 5 10 15
Ala Leu Ala Ser Pro Leu Ser Ala Arg Lys Gln Ala Ala Met Asp Ala
20 25 30
Leu Arg Ala Arg Arg Ser Phe Arg Arg Ser Asn Asn Thr Ala Gly Thr
35 40 45
Ala Asp Ala Thr Ser Pro Asn Trp Ala Gly Val Ala Ile Ser Gln Thr
50 55 60
Gly Val Thr Glu Val Ser Gly Thr Phe Thr Val Pro Gln Pro Lys Leu
65 70 75 80
Pro Ala Gly Gly Asp Pro Ser Val Ser Tyr Cys Gly Val Ala Trp Val
85 90 95
Gly Ile Asp Gly Trp Thr Asn Gly Asp Leu Ile Gln Thr Gly Val Leu
100 105 110
Trp Cys Ile Thr Glu Gly Glu Thr Phe Trp Asp Pro Trp Tyr Glu Tyr
115 120 125
Leu Pro Gly Ala Ile Thr Ile Tyr Glu Gly Val Thr Val Ser Ala Gly
130 135 140
Ser Val Val Thr Val Thr Ala Thr Lys Thr Gly Thr Asn Ser Gly Val
145 150 155 160
Thr Thr Leu Thr Val Asp Gly Ile Thr Val Ser His Thr Phe Thr Gly
165 170 175
Glu Asn Phe Ser Leu Pro Gly Thr Ser Ala Glu Trp Ile Val Glu Asp
180 185 190
Tyr Glu Tyr Ser Val Ala Gly Pro Pro Phe Val Pro Phe Ala Asp Phe
195 200 205
Gly Ser Val Thr Phe Thr Gly Ala Thr Ala Val Val Asn Gly Ala Thr
210 215 220
Ile Thr Pro Gly Glu Gly Asn Trp Val Ile Ile Asp Leu Glu Arg Ser
225 230 235 240
Asn Ser Ser Ile Thr Ser Ser Ser Phe Ser Gly Ser Thr Val Thr Ile
245 250 255
Asn Tyr Leu
<210> 133
<211> 271
<212> PRT
<213> 黄曲霉
<400> 133
Met Lys Leu Phe Ala Ile Leu Ser Ser Thr Val Leu Ala Ser Val Ala
1 5 10 15
Leu Ala Ser Pro Leu Thr Leu Glu Arg Arg Ala Arg Asn Ala Ala Arg
20 25 30
Leu Gln Ala Arg Val Ala Gln Arg His Ser Asn Leu Pro Phe Lys Ala
35 40 45
Gly Thr Asn Glu Ile Leu His Leu Asn Glu Thr Thr His Glu Glu Tyr
50 55 60
Ser Ser Asn Trp Ala Gly Ala Val Leu Ile Gly Ser Gly Tyr Thr Ser
65 70 75 80
Val Thr Gly Thr Phe Thr Val Pro Thr Pro Ser Ala Pro Ser Gly Ala
85 90 95
Asp Ser Asp Glu Gln Tyr Cys Ala Thr Ala Trp Val Gly Ile Asp Gly
100 105 110
Asp Thr Cys Gln Ser Ala Ile Leu Gln Thr Gly Val Asp Phe Cys Ile
115 120 125
Asp Ser Ser Gly Thr Thr Phe Asp Ala Trp Tyr Glu Trp Phe Pro Asp
130 135 140
Tyr Ser His Asp Phe Ser Gly Ile Ser Ile Ser Ala Gly Asp Lys Val
145 150 155 160
Lys Val Thr Val Asp Ala Ser Ser Lys Thr Ala Gly Thr Ala Ile Val
165 170 175
Glu Asn Leu Thr Thr Gly Lys Thr Val Ser His Thr Phe Thr Gly Gln
180 185 190
Asp Asp Asn Ala Leu Cys Glu Thr Asn Ala Glu Trp Ile Met Glu Asp
195 200 205
Phe Ser Ser Phe Leu Ser Leu Val Pro Phe Ala Asn Phe Gly Thr Val
210 215 220
Thr Phe Thr Asp Ile Ser Ala Thr Ser Gly Asp Ser Ser Val Gly Ala
225 230 235 240
Ser Asn Ala Thr Ile Ile Asp Ile Gln Gln Asn Asn Lys Thr Leu Thr
245 250 255
Ser Ser Ser Ala Ser Asp Thr Glu Val Thr Ile Lys Tyr Ile Gly
260 265 270
<210> 134
<211> 265
<212> PRT
<213> 黑曲霉
<400> 134
Met Lys Thr Thr Ala Leu Leu Thr Ala Gly Leu Leu Ala Thr Thr Ala
1 5 10 15
Met Ala Ala Pro Leu Thr Ala Lys Arg Gln Ala Ala Arg Ala Lys Arg
20 25 30
Ser Thr Asn Arg Gln Ser Asn Pro Pro Phe Lys Pro Gly Thr Asn Glu
35 40 45
Val Leu Ala Leu Asn Gly Thr Lys Asn Val Glu Tyr Ser Ser Asn Trp
50 55 60
Ala Gly Ala Val Leu Ile Gly Thr Gly Tyr Thr Ala Val Thr Ala Glu
65 70 75 80
Phe Val Val Pro Thr Pro Ser Val Pro Ser Gly Gly Ser Ser Arg Glu
85 90 95
Glu Tyr Cys Ala Ser Ala Trp Val Gly Ile Asp Gly Asp Thr Cys Asp
100 105 110
Thr Ala Ile Leu Gln Thr Gly Val Asp Phe Cys Val Gln Gly Ser Glu
115 120 125
Val Ser Phe Asp Ala Trp Tyr Glu Trp Tyr Pro Asp Tyr Ala Tyr Asp
130 135 140
Phe Ser Gly Ile Ser Ile Ser Ala Gly Asp Thr Ile Lys Val Thr Val
145 150 155 160
Asp Ala Ser Ser Asp Thr Thr Gly Thr Ala Thr Ile Glu Asn Val Ser
165 170 175
Thr Gly Thr Thr Val Thr His Ser Phe Thr Gly Gly Val Asp Gly Asp
180 185 190
Leu Cys Glu Tyr Asn Ala Glu Trp Ile Val Glu Asp Phe Glu Glu Asp
195 200 205
Asp Ser Leu Val Pro Phe Ala Asp Phe Gly Thr Val Thr Phe Thr Ser
210 215 220
Cys Ser Ala Thr Lys Asp Gly Ser Ser Val Gly Pro Glu Asp Ala Thr
225 230 235 240
Ile Ile Asp Ile Glu Gln Asn Glu Val Leu Thr Ser Val Ser Val Ser
245 250 255
Ser Ser Glu Val Val Val Lys Tyr Val
260 265
<210> 135
<211> 270
<212> PRT
<213> 构巢曲霉
<400> 135
Met Lys Leu Ser Ile Ala Ala Ile Ser Ser Ile Leu Leu Ala Gly Gly
1 5 10 15
Ala Leu Ala Ala Pro Leu Thr Glu Ala Arg Arg Gln Ala Arg Ala Leu
20 25 30
Arg Gly Arg Gly Pro Ser Asn Arg Ala Ser His Pro Pro Tyr Lys Val
35 40 45
Gly Ser Thr Glu Leu Leu Ser Leu Ser Asn Glu Thr Gln Val Glu Tyr
50 55 60
Ser Ser Asn Trp Ala Gly Ala Val Leu Ile Gly Thr Gly Tyr Thr Ser
65 70 75 80
Val Thr Gly Glu Phe Thr Val Pro Thr Pro Ser Leu Pro Thr Gly Ala
85 90 95
Ser Arg Asn Lys Gln Tyr Cys Ala Ser Ala Trp Val Gly Ile Asp Gly
100 105 110
Asp Thr Cys Ser Thr Ala Ile Leu Gln Thr Gly Ile Asp Phe Cys Ile
115 120 125
Gln Gly Ser Ser Val Ser Phe Asp Ala Trp Tyr Glu Trp Tyr Pro Asp
130 135 140
Tyr Ala Tyr Asp Phe Ser Gly Ile Thr Ile Ser Ala Gly Asp Ser Ile
145 150 155 160
Arg Val Thr Val Asp Ala Thr Thr Leu Thr Ala Gly Thr Ala Thr Val
165 170 175
Glu Asn Leu Thr Thr Gly Lys Thr Val Thr His Thr Phe Thr Gly Gly
180 185 190
Val Asp Gly Asp Leu Cys Glu Tyr Asn Ala Glu Trp Ile Val Glu Asp
195 200 205
Phe Glu Ser Asn Gly Gln Leu Val Pro Phe Ala Asp Phe Gly Thr Val
210 215 220
Thr Phe Thr Ser Ala Glu Val Thr Asn Asp Gly Lys Thr Val Gly Pro
225 230 235 240
Ser Gly Ala Thr Ile Ile Asp Ile Lys Gln Gly Asn Ser Val Leu Thr
245 250 255
Asp Val Ser Val Thr Asp Asp Ser Val Thr Val Ser Tyr Val
260 265 270
<210> 136
<211> 269
<212> PRT
<213> 棒曲霉
<400> 136
Met Lys Phe Ser Ala Val Val Ala Ser Ser Leu Leu Ala Gly Ala Ala
1 5 10 15
Ile Ala Ala Pro Leu Thr Glu Gln Arg Gln Ala Arg His Ala Arg Arg
20 25 30
Thr Ala Arg Ala Thr Asn Arg Ser Ser His Pro Pro Tyr Lys Val Gly
35 40 45
Ser Ser Glu Leu Leu Glu Leu Ser Asn Ser Thr His Val Glu Tyr Ser
50 55 60
Ser Asn Trp Ala Gly Ala Val Leu Ile Gly Asn Gly Tyr Thr Ser Val
65 70 75 80
Val Gly Glu Phe Thr Val Pro Glu Pro Ser Ala Pro Asp Gly Ala Asp
85 90 95
Ser Gly Thr Gln Tyr Cys Ala Ser Ala Trp Val Gly Ile Asp Gly Asp
100 105 110
Thr Cys Ser Ser Ala Ile Leu Gln Thr Gly Val Asp Phe Cys Ile Gln
115 120 125
Asp Gly Ser Val Ser Phe Asp Ala Trp Tyr Glu Trp Tyr Pro Asp Tyr
130 135 140
Ala Tyr Asp Phe Ser Gly Ile Asp Ile Ser Val Gly Asp Thr Ile Lys
145 150 155 160
Leu Thr Ile Asp Ala Thr Ser Lys Thr Ala Gly Ser Ala Ile Ile Gln
165 170 175
Asn Leu Ser Thr Gly Lys Thr Val Lys His Thr Phe Thr Gly Gly Val
180 185 190
Asp Gly Asp Leu Cys Glu Thr Asn Ala Glu Trp Ile Val Glu Asp Phe
195 200 205
Glu Ser Asn Gly Ser Leu Val Pro Phe Val Asn Phe Gly Thr Val Thr
210 215 220
Phe Thr Gly Ala Lys Ala Thr Thr Gly Gly Ser Ser Val Gly Pro Ser
225 230 235 240
Gly Ala Thr Leu Met Asp Ile Lys Gln Gly Ser Ser Val Leu Thr Ser
245 250 255
Ser Ser Val Ser Gly Asn Ser Val Ser Val Ser Tyr Val
260 265
<210> 137
<211> 269
<212> PRT
<213> 烟曲霉
<400> 137
Met Lys Phe Thr Ser Val Leu Ala Ser Gly Leu Leu Ala Thr Ala Ala
1 5 10 15
Ile Ala Ala Pro Leu Thr Glu Gln Arg Gln Ala Arg His Ala Arg Arg
20 25 30
Leu Ala Arg Thr Ala Asn Arg Ser Ser His Pro Pro Tyr Lys Pro Gly
35 40 45
Thr Ser Glu Val Ile Lys Leu Ser Asn Thr Thr Gln Val Glu Tyr Ser
50 55 60
Ser Asn Trp Ala Gly Ala Val Leu Ile Gly Thr Gly Tyr Thr Ala Val
65 70 75 80
Thr Gly Glu Phe Val Val Pro Thr Pro Ser Val Pro Ser Gly Gly Ser
85 90 95
Ser Ser Lys Gln Tyr Cys Ala Ser Ala Trp Val Gly Ile Asp Gly Asp
100 105 110
Thr Cys Ser Ser Ala Ile Leu Gln Thr Gly Val Asp Phe Cys Ile Gln
115 120 125
Gly Ser Ser Val Ser Phe Asp Ala Trp Tyr Glu Trp Tyr Pro Asp Tyr
130 135 140
Ala Tyr Asp Phe Ser Gly Ile Ser Ile Ser Ala Gly Asp Thr Ile Arg
145 150 155 160
Val Thr Val Asp Ala Thr Ser Lys Thr Ala Gly Thr Ala Thr Val Glu
165 170 175
Asn Val Thr Lys Gly Lys Thr Val Thr His Thr Phe Thr Gly Gly Val
180 185 190
Asp Gly Asn Leu Cys Glu Tyr Asn Ala Glu Trp Ile Val Glu Asp Phe
195 200 205
Glu Ser Asn Gly Ser Leu Val Pro Phe Ala Asn Phe Gly Thr Val Thr
210 215 220
Phe Thr Gly Ala Gln Ala Thr Asp Gly Gly Ser Thr Val Gly Pro Ser
225 230 235 240
Gly Ala Thr Leu Ile Asp Ile Gln Gln Ser Gly Lys Val Leu Thr Ser
245 250 255
Val Ser Thr Ser Ser Ser Ser Val Thr Val Lys Tyr Val
260 265
<210> 138
<211> 262
<212> PRT
<213> 马尼弗青霉菌
<400> 138
Met Lys Ser Ser Val Leu Ser Thr Leu Leu Leu Ala Gly Leu Ala Val
1 5 10 15
Ala Ala Pro Ser Thr Gln Lys Gly Leu Ala Arg Arg Ala Ala Arg Arg
20 25 30
Ser Asn Pro Ala Tyr Leu Pro Asn Ser Phe Gly Val Glu Gln Leu Asn
35 40 45
Ile Thr Gly Thr Lys His Val Glu Tyr Ser Ser Asn Trp Ser Gly Ala
50 55 60
Val Leu Ile Gly Thr Gly Tyr Ser Ala Val Thr Ala Glu Phe Thr Val
65 70 75 80
Pro Thr Pro His Ile Pro Ser Gly Gly Ser Ser Gly Thr Gln Tyr Cys
85 90 95
Ala Ser Ala Trp Val Gly Leu Asp Gly Asp Thr Cys Gly Thr Ser Ile
100 105 110
Leu Gln Thr Gly Val Asp Phe Cys Val Gln Gly Ser Ser Val Ser Tyr
115 120 125
Asp Ala Trp Tyr Glu Trp Tyr Pro Asp Tyr Ala Tyr Asp Phe Thr Gly
130 135 140
Ile Ser Ile Ser Ala Gly Asp Leu Ile Lys Ile Thr Val Thr Ala Thr
145 150 155 160
Ser Thr Ser Ser Gly Ser Ala Val Val Gln Asn Val Ser Lys Gly Thr
165 170 175
Thr Val Thr His Asn Phe Ser Gly Glu Ser Asp Lys Leu Cys Glu Tyr
180 185 190
Asn Ala Glu Trp Ile Val Glu Asp Phe Glu Glu Gly Gly Ser Leu Val
195 200 205
Pro Phe Ala Asn Phe Gly Thr Val Thr Phe Ser Gly Ala Lys Ala Thr
210 215 220
Lys Ser Gly Ser Ser Val Asp Thr Thr Gly Ala Thr Ile Ile Asp Ile
225 230 235 240
Glu Gln Asn Ser Ser Val Leu Thr Ser Val Thr Ala Ser Gly Ser Thr
245 250 255
Val Thr Val Lys Tyr Val
260
<210> 139
<211> 274
<212> PRT
<213> 柄篮状菌
<400> 139
Met Lys Ser Ile Val Leu Ser Leu Leu Ile Leu Ala Gly Leu Thr Ala
1 5 10 15
Ala Ala Pro Leu Thr Glu Lys Arg Leu Ala Arg Arg Ala Ala Gly Thr
20 25 30
Arg Arg Ser Ser Pro Pro Tyr Val Pro Asn Ser Tyr Gly Val Glu Gln
35 40 45
Leu Asn Ile Thr Asn Ser Lys Asn Val Glu Tyr Ser Glu Asn Trp Ala
50 55 60
Gly Ala Val Leu Ile Gly Thr Gly Tyr Thr Ala Val Thr Ala Glu Phe
65 70 75 80
Thr Val Pro Thr Pro Thr Ala Pro Gly Gly Gly Asn Thr Glu Tyr Cys
85 90 95
Ala Ser Ala Trp Val Gly Leu Asp Gly Asp Thr Cys Asp Ser Ser Ile
100 105 110
Leu Gln Thr Gly Val Asp Phe Cys Val Gln Gly Gly Asp Val Ser Tyr
115 120 125
Asp Ala Trp Tyr Glu Trp Tyr Pro Asp Tyr Ala Tyr Asp Phe Ser Gly
130 135 140
Ile Ser Ile Ser Ala Gly Asp Val Met Lys Ile Thr Val Thr Ala Thr
145 150 155 160
Ser Thr Ser Ser Gly Ser Ala Val Val Glu Asn Val Ser Thr Gly Arg
165 170 175
Thr Val Thr His Ser Phe Ser Gly Glu Ser Asp Arg Leu Cys Glu Tyr
180 185 190
Asn Ala Glu Trp Ile Val Glu Asp Phe Glu Glu Gly Asp Ser Leu Val
195 200 205
Pro Phe Ala Asn Phe Gly Thr Val Thr Phe Thr Asn Ala Val Ala Thr
210 215 220
His Asn Gly Ala Gly Val Gly Thr Ser Gly Ala Thr Ile Ile Asp Ile
225 230 235 240
Glu Gln Asp Ser Gly Val Leu Thr Ser Val Ser Ala Ser Gly Asn Thr
245 250 255
Val Thr Val Asn Met Lys His Thr Asn Thr Asp Ile His Asp Asp Gly
260 265 270
Gly Gly
<210> 140
<211> 274
<212> PRT
<213> Grosmannia clavigera
<400> 140
Met Lys Leu Leu Asn Val Ala Gly Ala Leu Leu Phe Ala Asn Ala Ala
1 5 10 15
Leu Gly Ala Arg Phe Thr Glu Arg Arg Arg Ala Arg Leu Ala Gln Arg
20 25 30
Ala Pro Gly Ser Val Ser Arg Thr Gly Phe Gly Gln Pro Leu Ile Ala
35 40 45
Ala Asp Gly Leu Asp Ile Asp Glu Asp Ala Ala Thr Asn Gly Thr Ala
50 55 60
Asp Val Asp Tyr Ser Ser Asn Trp Ser Gly Ala Val Leu Ile Ser Thr
65 70 75 80
Gly Tyr Lys Ser Val Thr Gly Thr Phe Val Val Pro Thr Pro Lys Ala
85 90 95
Pro Thr Gly Gly Ser Ser Ser Thr Gln Tyr Ala Ala Ser Ala Trp Val
100 105 110
Gly Ile Asp Gly Asp Thr Ala Ser Asn Ser Ile Leu Gln Thr Gly Ile
115 120 125
Asp Phe Tyr Ile Glu Asp Gly Glu Ala Ser Tyr Asp Ala Trp Tyr Glu
130 135 140
Trp Tyr Pro Asp Tyr Ala Tyr Thr Phe Ser Gly Phe Ser Ile Ser Ala
145 150 155 160
Gly Asp Thr Ile Thr Val Thr Val Thr Ala Ser Ser Thr Ser Ser Gly
165 170 175
Lys Ala Val Ile Lys Asn Ala Ser Thr Gly Lys Ser Val Thr His Ser
180 185 190
Phe Ser Ser Glu Ser Asn Thr Leu Glu Glu Leu Asn Ala Glu Trp Ile
195 200 205
Val Glu Asp Phe Glu Glu Asp Gly Gly Leu Val Pro Phe Ala Asn Phe
210 215 220
Gly Thr Val Thr Phe Thr Asp Ala Thr Ala Ile Gly Ser Asp Gly Thr
225 230 235 240
Val Gly Pro Asp Gly Ala Thr Leu Ile Asp Ile Arg Gln Ser Gly Lys
245 250 255
Val Leu Thr Ser Ala Ser Leu Ser Gly Gly Asp Val Val Val Ser Tyr
260 265 270
Val Gly
<210> 141
<211> 269
<212> PRT
<213> 费希新萨托菌
<400> 141
Met Lys Phe Thr Ser Val Leu Thr Ser Gly Leu Leu Ala Thr Ala Thr
1 5 10 15
Ile Ala Ala Pro Leu Thr Glu Gln Arg Gln Ala Arg His Ala Arg Arg
20 25 30
Met Ala Arg Asn Ser Asn Arg Ser Ser His Pro Pro Tyr Lys Pro Gly
35 40 45
Thr Ser Glu Val Ile Lys Leu Ser Asn Thr Thr Gln Val Glu Tyr Ser
50 55 60
Ser Asn Trp Ala Gly Ala Val Leu Ile Gly Ser Gly Tyr Thr Ala Val
65 70 75 80
Thr Gly Glu Phe Val Val Pro Thr Pro His Ala Pro Ser Gly Gly Ser
85 90 95
Ser Asn Glu Gln Tyr Cys Ala Ser Ala Trp Val Gly Ile Asp Gly Asp
100 105 110
Thr Cys Ser Ser Ala Ile Leu Gln Thr Gly Val Asp Phe Cys Ile Gln
115 120 125
Gly Gly Ser Val Ser Phe Asp Ala Trp Tyr Glu Trp Tyr Pro Asp Tyr
130 135 140
Ala Tyr Asp Phe Ser Gly Ile Ser Ile Ser Ala Gly Asp Thr Ile Arg
145 150 155 160
Val Thr Val Asp Ala Thr Ser Lys Thr Ala Gly Thr Ala Thr Val Glu
165 170 175
Asn Val Thr Lys Gly Lys Thr Val Thr His Thr Phe Thr Gly Gly Val
180 185 190
Asp Gly Asn Leu Cys Glu Tyr Asn Ala Glu Trp Ile Val Glu Asp Phe
195 200 205
Glu Ser Asn Gly Ser Leu Val Pro Phe Ala Asn Phe Gly Thr Val Thr
210 215 220
Phe Thr Gly Ala Gln Ala Thr Asp Gly Gly Ser Thr Val Gly Pro Ser
225 230 235 240
Gly Ala Thr Leu Met Asp Ile Glu Gln Asn Gly Gln Val Leu Thr Ser
245 250 255
Val Ser Ser Ser Ser Ser Ser Val Thr Val Lys Tyr Val
260 265
<210> 142
<211> 270
<212> PRT
<213> 马尼弗青霉菌
<400> 142
Met Ile Phe Ser Ile Lys Thr Leu Val Pro Leu Leu Leu Ala Gly Thr
1 5 10 15
Ala Ile Ala Ser Pro Ala Thr Ile Pro Ala Ala Arg Arg Phe Arg Gly
20 25 30
Gly Ser Asp Ala Ser Phe Ala Asn Phe Thr Ser Ser Ala Asp Glu Ala
35 40 45
Ser Ala Ala Thr Leu Gly Thr Tyr Thr Phe Thr Asn Trp Ala Gly Ala
50 55 60
Phe Leu Arg Asn Thr Ala Val Thr Ser Val Thr Gly Thr Phe Val Ile
65 70 75 80
Pro Lys Pro Ser Met Pro Ala Gly Gly Asn Ser Gly Thr Ser Tyr Cys
85 90 95
Gly Cys Ala Trp Val Gly Leu Asp Gly Ile Ser Asn Phe Cys Pro Asn
100 105 110
Gly Gly Leu Ile Gln Ala Gly Val Asn Trp Cys Val Glu Asn Gly Val
115 120 125
Thr Ser Phe Ala Ala Trp His Glu Trp Trp Pro Ala Glu Ala Met Gln
130 135 140
Tyr Trp Asp Asn Val Ser Val Ser Ala Gly Asp Ser Ile Thr Val Thr
145 150 155 160
Ile Thr Ala Ser Asn Thr Glu Ala Gly Ser Ala Thr Leu Ile Asn Asn
165 170 175
Thr Thr Gly Gln Arg Val Arg Phe Val Trp Thr Tyr Lys Thr Pro Ser
180 185 190
Leu Cys Glu Ala Thr Ala Glu Trp Ile Val Glu Asp Tyr Ser Ile Gly
195 200 205
Asn Asn Leu Val Ala Phe Ala Asp Tyr Gly Ser Val Thr Phe Thr Gly
210 215 220
Asn Ser Ala Val Ile His Gly Ala Thr Thr Ser Asp Leu Ser Asn Ser
225 230 235 240
Tyr Ile Ala Asn Met Val Ser Val Ser Asn Pro Ser Gln Val Ile Ser
245 250 255
Ala Ser Thr Val Asn Gly Asn Ala Ile Thr Val Val Tyr Gln
260 265 270
<210> 143
<211> 270
<212> PRT
<213> 产黄青霉
<400> 143
Met Lys Phe Ser Thr Thr Ile Leu Ser Ala Ala Leu Leu Thr Ala Ser
1 5 10 15
Ala Ile Ala Ala Pro Leu Thr Glu Arg Arg Ala Ala Arg His Ala Ala
20 25 30
Arg Ala Ala Asp Arg Ala Gly Arg Ile Ser Arg Pro Ala Ala Lys Gln
35 40 45
Asp Thr Leu Glu Leu Val Ser Leu Asn Gly Thr Ser His Ala Gln Tyr
50 55 60
Ser Ser Asn Trp Ala Gly Ala Val Leu Ile Gly Thr Gly Tyr Thr Ser
65 70 75 80
Val Thr Gly Glu Phe Thr Val Pro Lys Pro Asn Leu Pro Ser Asp Ala
85 90 95
Ser Ser Gly Gln Gln Ser Cys Ala Ser Ala Trp Val Gly Ile Asp Gly
100 105 110
Asp Thr Cys Ser Ser Ala Ile Leu Gln Thr Gly Ile Asp Phe Cys Ile
115 120 125
Gln Gly Gly Glu Val Ser Tyr Asp Ala Trp Tyr Glu Trp Tyr Pro Asp
130 135 140
Tyr Ala His Asp Phe Ser Gly Ile Asp Ile Ser Thr Gly Asp Val Ile
145 150 155 160
Lys Leu Thr Val Thr Ala Thr Ser Lys Ser Ser Gly Ser Ala Val Ile
165 170 175
Asp Asn Val Thr Thr Gly Lys Ser Val Thr His Lys Phe Ser Gly Val
180 185 190
Asp Asp Gly Asp Leu Cys Glu Thr Asn Ala Glu Trp Ile Val Glu Asp
195 200 205
Phe Glu Ser Gly Gly Gln Leu Val Pro Phe Thr Asn Phe Gly Thr Val
210 215 220
Thr Phe Thr Lys Ala Glu Ala Thr Ser Gly Gly Lys Ser Val Gly Pro
225 230 235 240
Ser Gly Ala Thr Leu Phe Glu Ile Arg Gln Ser Lys Lys Val Leu Thr
245 250 255
Lys Ser Ser Val Thr Gly Asn Ser Val Thr Val Ser Tyr Val
260 265 270
<210> 144
<211> 282
<212> PRT
<213> 黑曲霉
<400> 144
Met Lys Phe Ser Thr Ile Leu Thr Gly Ser Leu Phe Ala Thr Ala Ala
1 5 10 15
Leu Ala Ala Pro Leu Thr Glu Lys Arg Arg Ala Arg Lys Glu Ala Arg
20 25 30
Ala Ala Gly Lys Arg His Ser Asn Pro Pro Tyr Ile Pro Gly Ser Asp
35 40 45
Lys Glu Ile Leu Lys Leu Asn Gly Thr Thr Asn Glu Glu Tyr Ser Ser
50 55 60
Asn Trp Ala Gly Ala Val Leu Ile Gly Asp Gly Tyr Thr Lys Val Thr
65 70 75 80
Gly Glu Phe Thr Val Pro Ser Val Ser Ala Gly Ser Ser Gly Ser Ser
85 90 95
Gly Tyr Gly Gly Gly Tyr Gly Tyr Trp Lys Asn Lys Arg Gln Ser Glu
100 105 110
Glu Tyr Cys Ala Ser Ala Trp Val Gly Ile Asp Gly Asp Thr Cys Glu
115 120 125
Thr Ala Ile Leu Gln Thr Gly Val Asp Phe Cys Tyr Glu Asp Gly Gln
130 135 140
Thr Ser Tyr Asp Ala Trp Tyr Glu Trp Tyr Pro Asp Tyr Ala Tyr Asp
145 150 155 160
Phe Ser Asp Ile Thr Ile Ser Glu Gly Asp Ser Ile Lys Val Thr Val
165 170 175
Glu Ala Thr Ser Lys Ser Ser Gly Ser Ala Thr Val Glu Asn Leu Thr
180 185 190
Thr Gly Gln Ser Val Thr His Thr Phe Ser Gly Asn Val Glu Gly Asp
195 200 205
Leu Cys Glu Thr Asn Ala Glu Trp Ile Val Glu Asp Phe Glu Ser Gly
210 215 220
Asp Ser Leu Val Ala Phe Ala Asp Phe Gly Ser Val Thr Phe Thr Asn
225 230 235 240
Ala Glu Ala Thr Ser Gly Gly Ser Thr Val Gly Pro Ser Asp Ala Thr
245 250 255
Val Met Asp Ile Glu Gln Asp Gly Ser Val Leu Thr Glu Thr Ser Val
260 265 270
Ser Gly Asp Ser Val Thr Val Thr Tyr Val
275 280
<210> 145
<211> 612
<212> PRT
<213> 里氏木霉
<400> 145
Met Ala Lys Leu Ser Thr Leu Arg Leu Ala Ser Leu Leu Ser Leu Val
1 5 10 15
Ser Val Gln Val Ser Ala Ser Val His Leu Leu Glu Ser Leu Glu Lys
20 25 30
Leu Pro His Gly Trp Lys Ala Ala Glu Thr Pro Ser Pro Ser Ser Gln
35 40 45
Ile Val Leu Gln Val Ala Leu Thr Gln Gln Asn Ile Asp Gln Leu Glu
50 55 60
Ser Arg Leu Ala Ala Val Ser Thr Pro Thr Ser Ser Thr Tyr Gly Lys
65 70 75 80
Tyr Leu Asp Val Asp Glu Ile Asn Ser Ile Phe Ala Pro Ser Asp Ala
85 90 95
Ser Ser Ser Ala Val Glu Ser Trp Leu Gln Ser His Gly Val Thr Ser
100 105 110
Tyr Thr Lys Gln Gly Ser Ser Ile Trp Phe Gln Thr Asn Ile Ser Thr
115 120 125
Ala Asn Ala Met Leu Ser Thr Asn Phe His Thr Tyr Ser Asp Leu Thr
130 135 140
Gly Ala Lys Lys Val Arg Thr Leu Lys Tyr Ser Ile Pro Glu Ser Leu
145 150 155 160
Ile Gly His Val Asp Leu Ile Ser Pro Thr Thr Tyr Phe Gly Thr Thr
165 170 175
Lys Ala Met Arg Lys Leu Lys Ser Ser Gly Val Ser Pro Ala Ala Asp
180 185 190
Ala Leu Ala Ala Arg Gln Glu Pro Ser Ser Cys Lys Gly Thr Leu Val
195 200 205
Phe Glu Gly Glu Thr Phe Asn Val Phe Gln Pro Asp Cys Leu Arg Thr
210 215 220
Glu Tyr Ser Val Asp Gly Tyr Thr Pro Ser Val Lys Ser Gly Ser Arg
225 230 235 240
Ile Gly Phe Gly Ser Phe Leu Asn Glu Ser Ala Ser Phe Ala Asp Gln
245 250 255
Ala Leu Phe Glu Lys His Phe Asn Ile Pro Ser Gln Asn Phe Ser Val
260 265 270
Val Leu Ile Asn Gly Gly Thr Asp Leu Pro Gln Pro Pro Ser Asp Ala
275 280 285
Asn Asp Gly Glu Ala Asn Leu Asp Ala Gln Thr Ile Leu Thr Ile Ala
290 295 300
His Pro Leu Pro Ile Thr Glu Phe Ile Thr Ala Gly Ser Pro Pro Tyr
305 310 315 320
Phe Pro Asp Pro Val Glu Pro Ala Gly Thr Pro Asn Glu Asn Glu Pro
325 330 335
Tyr Leu Gln Tyr Tyr Glu Phe Leu Leu Ser Lys Ser Asn Ala Glu Ile
340 345 350
Pro Gln Val Ile Thr Asn Ser Tyr Gly Asp Glu Glu Gln Thr Val Pro
355 360 365
Arg Ser Tyr Ala Val Arg Val Cys Asn Leu Ile Gly Leu Leu Gly Leu
370 375 380
Arg Gly Ile Ser Val Leu His Ser Ser Gly Asp Glu Gly Val Gly Ala
385 390 395 400
Ser Cys Val Ala Thr Asn Ser Thr Thr Pro Gln Phe Asn Pro Ile Phe
405 410 415
Pro Ala Thr Cys Pro Tyr Val Thr Ser Val Gly Gly Thr Val Ser Phe
420 425 430
Asn Pro Glu Val Ala Trp Ala Gly Ser Ser Gly Gly Phe Ser Tyr Tyr
435 440 445
Phe Ser Arg Pro Trp Tyr Gln Gln Glu Ala Val Gly Thr Tyr Leu Glu
450 455 460
Lys Tyr Val Ser Ala Glu Thr Lys Lys Tyr Tyr Gly Pro Tyr Val Asp
465 470 475 480
Phe Ser Gly Arg Gly Phe Pro Asp Val Ala Ala His Ser Val Ser Pro
485 490 495
Asp Tyr Pro Val Phe Gln Gly Gly Glu Leu Thr Pro Ser Gly Gly Thr
500 505 510
Ser Ala Ala Ser Pro Val Val Ala Ala Ile Val Ala Leu Leu Asn Asp
515 520 525
Ala Arg Leu Arg Glu Gly Lys Pro Thr Leu Gly Phe Leu Asn Pro Leu
530 535 540
Ile Tyr Leu His Ala Ser Lys Gly Phe Thr Asp Ile Thr Ser Gly Gln
545 550 555 560
Ser Glu Gly Cys Asn Gly Asn Asn Thr Gln Thr Gly Ser Pro Leu Pro
565 570 575
Gly Ala Gly Phe Ile Ala Gly Ala His Trp Asn Ala Thr Lys Gly Trp
580 585 590
Asp Pro Thr Thr Gly Phe Gly Val Pro Asn Leu Lys Lys Leu Leu Ala
595 600 605
Leu Val Arg Phe
610
<210> 146
<211> 609
<212> PRT
<213> 深绿木霉
<400> 146
Met Ala Lys Leu Thr Ala Leu Ala Gly Leu Leu Thr Leu Ala Ser Val
1 5 10 15
Gln Ala Asn Ala Ala Val Leu Leu Asp Ser Leu Asp Lys Val Pro Val
20 25 30
Gly Trp Gln Ala Ala Ser Ala Pro Ala Pro Ser Ser Lys Ile Thr Leu
35 40 45
Gln Val Ala Leu Thr Gln Gln Asn Ile Asp Gln Leu Glu Ser Lys Leu
50 55 60
Ala Ala Val Ser Thr Pro Asn Ser Ser Asn Tyr Gly Lys Tyr Leu Asp
65 70 75 80
Val Asp Glu Ile Asn Gln Ile Phe Ala Pro Ser Ser Ala Ser Thr Ala
85 90 95
Ala Val Glu Ser Trp Leu Lys Ser Tyr Gly Val Asp Tyr Lys Val Gln
100 105 110
Gly Ser Ser Ile Trp Phe Gln Thr Asp Val Ser Thr Ala Asn Lys Met
115 120 125
Leu Ser Thr Asn Phe His Thr Tyr Thr Asp Ser Val Gly Ala Lys Lys
130 135 140
Val Arg Thr Leu Gln Tyr Ser Val Pro Glu Thr Leu Ala Asp His Ile
145 150 155 160
Asp Leu Ile Ser Pro Thr Thr Tyr Phe Gly Thr Ser Lys Ala Met Arg
165 170 175
Ala Leu Lys Ile Gln Asn Ala Ala Ser Ala Val Ser Pro Leu Ala Ala
180 185 190
Arg Gln Glu Pro Ser Ser Cys Lys Gly Thr Ile Glu Phe Glu Asn Arg
195 200 205
Thr Phe Asn Val Phe Gln Pro Asp Cys Leu Arg Thr Glu Tyr Ser Val
210 215 220
Asn Gly Tyr Lys Pro Ser Ala Lys Ser Gly Ser Arg Ile Gly Phe Gly
225 230 235 240
Ser Phe Leu Asn Gln Ser Ala Ser Ser Ser Asp Leu Ala Leu Phe Glu
245 250 255
Lys His Phe Gly Phe Ala Ser Gln Gly Phe Ser Val Glu Leu Ile Asn
260 265 270
Gly Gly Ser Asn Pro Gln Pro Pro Thr Asp Ala Asn Asp Gly Glu Ala
275 280 285
Asn Leu Asp Ala Gln Asn Ile Val Ser Phe Val Gln Pro Leu Pro Ile
290 295 300
Thr Glu Phe Ile Ala Gly Gly Thr Ala Pro Tyr Phe Pro Asp Pro Val
305 310 315 320
Glu Pro Ala Gly Thr Pro Asp Glu Asn Glu Pro Tyr Leu Glu Tyr Tyr
325 330 335
Glu Tyr Leu Leu Ser Lys Ser Asn Lys Glu Leu Pro Gln Val Ile Thr
340 345 350
Asn Ser Tyr Gly Asp Glu Glu Gln Thr Val Pro Gln Ala Tyr Ala Val
355 360 365
Arg Val Cys Asn Leu Ile Gly Leu Met Gly Leu Arg Gly Ile Ser Ile
370 375 380
Leu Glu Ser Ser Gly Asp Glu Gly Val Gly Ala Ser Cys Leu Ala Thr
385 390 395 400
Asn Ser Thr Thr Thr Pro Gln Phe Asn Pro Ile Phe Pro Ala Thr Cys
405 410 415
Pro Tyr Val Thr Ser Val Gly Gly Thr Val Ser Phe Asn Pro Glu Val
420 425 430
Ala Trp Asp Gly Ser Ser Gly Gly Phe Ser Tyr Tyr Phe Ser Arg Pro
435 440 445
Trp Tyr Gln Glu Ala Ala Val Gly Thr Tyr Leu Asn Lys Tyr Val Ser
450 455 460
Glu Glu Thr Lys Glu Tyr Tyr Lys Ser Tyr Val Asp Phe Ser Gly Arg
465 470 475 480
Gly Phe Pro Asp Val Ala Ala His Ser Val Ser Pro Asp Tyr Pro Val
485 490 495
Phe Gln Gly Gly Glu Leu Thr Pro Ser Gly Gly Thr Ser Ala Ala Ser
500 505 510
Pro Ile Val Ala Ser Val Ile Ala Leu Leu Asn Asp Ala Arg Leu Arg
515 520 525
Ala Gly Lys Pro Ala Leu Gly Phe Leu Asn Pro Leu Ile Tyr Gly Tyr
530 535 540
Ala Tyr Lys Gly Phe Thr Asp Ile Thr Ser Gly Gln Ala Val Gly Cys
545 550 555 560
Asn Gly Asn Asn Thr Gln Thr Gly Gly Pro Leu Pro Gly Ala Gly Val
565 570 575
Ile Pro Gly Ala Phe Trp Asn Ala Thr Lys Gly Trp Asp Pro Thr Thr
580 585 590
Gly Phe Gly Val Pro Asn Phe Lys Lys Leu Leu Glu Leu Val Arg Tyr
595 600 605
Ile
<210> 147
<211> 612
<212> PRT
<213> 绿木霉
<400> 147
Met Pro Lys Ser Thr Ala Leu Arg Leu Val Ser Leu Leu Ser Leu Ala
1 5 10 15
Ser Val Pro Ile Ser Ala Ser Val Leu Val Glu Ser Leu Glu Lys Leu
20 25 30
Pro His Gly Trp Lys Ala Ala Ser Ala Pro Ser Pro Ser Ser Gln Ile
35 40 45
Thr Leu Gln Val Ala Leu Thr Gln Gln Asn Ile Asp Gln Leu Glu Ser
50 55 60
Arg Leu Ala Ala Val Ser Thr Pro Asn Ser Lys Thr Tyr Gly Asn Tyr
65 70 75 80
Leu Asp Leu Asp Glu Ile Asn Glu Ile Phe Ala Pro Ser Asp Ala Ser
85 90 95
Ser Ala Ala Val Glu Ser Trp Leu His Ser His Gly Val Thr Lys Tyr
100 105 110
Thr Lys Gln Gly Ser Ser Ile Trp Phe Gln Thr Glu Val Ser Thr Ala
115 120 125
Asn Ala Met Leu Ser Thr Asn Phe His Thr Tyr Ser Asp Ala Ala Gly
130 135 140
Val Lys Lys Leu Arg Thr Leu Gln Tyr Ser Ile Pro Glu Ser Leu Val
145 150 155 160
Gly His Val Asp Leu Ile Ser Pro Thr Thr Tyr Phe Gly Thr Ser Asn
165 170 175
Ala Met Arg Ala Leu Arg Ser Lys Ser Val Ala Ser Val Ala Gln Ser
180 185 190
Val Ala Ala Arg Gln Glu Pro Ser Ser Cys Lys Gly Thr Leu Val Phe
195 200 205
Glu Gly Arg Thr Phe Asn Val Phe Gln Pro Asp Cys Leu Arg Thr Glu
210 215 220
Tyr Asn Val Asn Gly Tyr Thr Pro Ser Ala Lys Ser Gly Ser Arg Ile
225 230 235 240
Gly Phe Gly Ser Phe Leu Asn Gln Ser Ala Ser Phe Ser Asp Leu Ala
245 250 255
Leu Phe Glu Lys His Phe Gly Phe Ser Ser Gln Asn Phe Ser Val Val
260 265 270
Leu Ile Asn Gly Gly Thr Asp Leu Pro Gln Pro Pro Ser Asp Asp Asn
275 280 285
Asp Gly Glu Ala Asn Leu Asp Val Gln Asn Ile Leu Thr Ile Ala His
290 295 300
Pro Leu Pro Ile Thr Glu Phe Ile Thr Ala Gly Ser Pro Pro Tyr Phe
305 310 315 320
Pro Asp Pro Val Glu Pro Ala Gly Thr Pro Asp Glu Asn Glu Pro Tyr
325 330 335
Leu Gln Tyr Phe Glu Tyr Leu Leu Ser Lys Pro Asn Arg Asp Leu Pro
340 345 350
Gln Val Ile Thr Asn Ser Tyr Gly Asp Glu Glu Gln Thr Val Pro Gln
355 360 365
Ala Tyr Ala Val Arg Val Cys Asn Leu Ile Gly Leu Met Gly Leu Arg
370 375 380
Gly Ile Ser Ile Leu Glu Ser Ser Gly Asp Glu Gly Val Gly Ala Ser
385 390 395 400
Cys Val Ala Thr Asn Ser Thr Thr Pro Gln Phe Asn Pro Ile Phe Pro
405 410 415
Ala Thr Cys Pro Tyr Val Thr Ser Val Gly Gly Thr Val Asn Phe Asn
420 425 430
Pro Glu Val Ala Trp Asp Gly Ser Ser Gly Gly Phe Ser Tyr Tyr Phe
435 440 445
Ser Arg Pro Trp Tyr Gln Glu Glu Ala Val Gly Asn Tyr Leu Glu Lys
450 455 460
His Val Ser Ala Glu Thr Lys Lys Tyr Tyr Gly Pro Tyr Val Asp Phe
465 470 475 480
Ser Gly Arg Gly Phe Pro Asp Val Ala Ala His Ser Val Ser Pro Asp
485 490 495
Tyr Pro Val Phe Gln Gly Gly Gln Leu Thr Pro Ser Gly Gly Thr Ser
500 505 510
Ala Ala Ser Pro Val Val Ala Ser Ile Ile Ala Leu Leu Asn Asp Ala
515 520 525
Arg Leu Arg Glu Gly Lys Pro Thr Leu Gly Phe Leu Asn Pro Leu Ile
530 535 540
Tyr Gln Tyr Ala Tyr Lys Gly Phe Thr Asp Ile Thr Ser Gly Gln Ser
545 550 555 560
Asp Gly Cys Asn Gly Asn Asn Thr Gln Thr Asp Ala Pro Leu Pro Gly
565 570 575
Ala Gly Val Val Leu Gly Ala His Trp Asn Ala Thr Lys Gly Trp Asp
580 585 590
Pro Thr Thr Gly Phe Gly Val Pro Asn Phe Lys Lys Leu Leu Glu Leu
595 600 605
Ile Arg Tyr Ile
610
<210> 148
<211> 594
<212> PRT
<213> 烟曲霉
<400> 148
Met Leu Ser Ser Thr Leu Tyr Ala Gly Trp Leu Leu Ser Leu Ala Ala
1 5 10 15
Pro Ala Leu Cys Val Val Gln Glu Lys Leu Ser Ala Val Pro Ser Gly
20 25 30
Trp Thr Leu Ile Glu Asp Ala Ser Glu Ser Asp Thr Ile Thr Leu Ser
35 40 45
Ile Ala Leu Ala Arg Gln Asn Leu Asp Gln Leu Glu Ser Lys Leu Thr
50 55 60
Thr Leu Ala Thr Pro Gly Asn Pro Glu Tyr Gly Lys Trp Leu Asp Gln
65 70 75 80
Ser Asp Ile Glu Ser Leu Phe Pro Thr Ala Ser Asp Asp Ala Val Leu
85 90 95
Gln Trp Leu Lys Ala Ala Gly Ile Thr Gln Val Ser Arg Gln Gly Ser
100 105 110
Leu Val Asn Phe Ala Thr Thr Val Gly Thr Ala Asn Lys Leu Phe Asp
115 120 125
Thr Lys Phe Ser Tyr Tyr Arg Asn Gly Ala Ser Gln Lys Leu Arg Thr
130 135 140
Thr Gln Tyr Ser Ile Pro Asp His Leu Thr Glu Ser Ile Asp Leu Ile
145 150 155 160
Ala Pro Thr Val Phe Phe Gly Lys Glu Gln Asn Ser Ala Leu Ser Ser
165 170 175
His Ala Val Lys Leu Pro Ala Leu Pro Arg Arg Ala Ala Thr Asn Ser
180 185 190
Ser Cys Ala Asn Leu Ile Thr Pro Asp Cys Leu Val Glu Met Tyr Asn
195 200 205
Leu Gly Asp Tyr Lys Pro Asp Ala Ser Ser Gly Ser Arg Val Gly Phe
210 215 220
Gly Ser Phe Leu Asn Glu Ser Ala Asn Tyr Ala Asp Leu Ala Ala Tyr
225 230 235 240
Glu Gln Leu Phe Asn Ile Pro Pro Gln Asn Phe Ser Val Glu Leu Ile
245 250 255
Asn Arg Gly Val Asn Asp Gln Asn Trp Ala Thr Ala Ser Leu Gly Glu
260 265 270
Ala Asn Leu Asp Val Glu Leu Ile Val Ala Val Ser His Pro Leu Pro
275 280 285
Val Val Glu Phe Ile Thr Gly Gly Ser Pro Pro Phe Val Pro Asn Ala
290 295 300
Asp Glu Pro Thr Ala Ala Asp Asn Gln Asn Glu Pro Tyr Leu Gln Tyr
305 310 315 320
Tyr Glu Tyr Leu Leu Ser Lys Pro Asn Ser His Leu Pro Gln Val Ile
325 330 335
Ser Asn Ser Tyr Gly Asp Asp Glu Gln Thr Val Pro Glu Tyr Tyr Ala
340 345 350
Arg Arg Val Cys Asn Leu Ile Gly Leu Met Gly Leu Arg Gly Ile Thr
355 360 365
Val Leu Glu Ser Ser Gly Asp Thr Gly Ile Gly Ser Ala Cys Met Ser
370 375 380
Asn Asp Gly Thr Asn Lys Pro Gln Phe Thr Pro Thr Phe Pro Gly Thr
385 390 395 400
Cys Pro Phe Ile Thr Ala Val Gly Gly Thr Gln Ser Tyr Ala Pro Glu
405 410 415
Val Ala Trp Asp Gly Ser Ser Gly Gly Phe Ser Asn Tyr Phe Ser Arg
420 425 430
Pro Trp Tyr Gln Ser Phe Ala Val Asp Asn Tyr Leu Asn Asn His Ile
435 440 445
Thr Lys Asp Thr Lys Lys Tyr Tyr Ser Gln Tyr Thr Asn Phe Lys Gly
450 455 460
Arg Gly Phe Pro Asp Val Ser Ala His Ser Leu Thr Pro Tyr Tyr Glu
465 470 475 480
Val Val Leu Thr Gly Lys His Tyr Lys Ser Gly Gly Thr Ser Ala Ala
485 490 495
Ser Pro Val Phe Ala Gly Ile Val Gly Leu Leu Asn Asp Ala Arg Leu
500 505 510
Arg Ala Gly Lys Ser Thr Leu Gly Phe Leu Asn Pro Leu Leu Tyr Ser
515 520 525
Ile Leu Ala Glu Gly Phe Thr Asp Ile Thr Ala Gly Ser Ser Ile Gly
530 535 540
Cys Asn Gly Ile Asn Pro Gln Thr Gly Lys Pro Val Pro Gly Gly Gly
545 550 555 560
Ile Ile Pro Tyr Ala His Trp Asn Ala Thr Ala Gly Trp Asp Pro Val
565 570 575
Thr Gly Leu Gly Val Pro Asp Phe Met Lys Leu Lys Glu Leu Val Leu
580 585 590
Ser Leu
<210> 149
<211> 594
<212> PRT
<213> 米曲霉
<400> 149
Met Arg Ser Ser Phe Leu Leu Ala Gly Val Phe Gly Ser Leu Ala Ala
1 5 10 15
Pro Ala Leu Ala Ile Val His Glu Lys Val Ala Ala Val Pro Asn Gly
20 25 30
Trp Thr Leu Val Lys Gln Ala Pro Glu Ser Asp Pro Ile Thr Leu Ser
35 40 45
Ile Ala Leu Ala Arg Gln Asn Leu Asp Gln Leu Glu Ser Lys Leu Thr
50 55 60
Ala Leu Ser Thr Pro Gly Asn Ala Glu Tyr Gly Gln Trp Leu Asp Gln
65 70 75 80
Ser Gly Ile Glu Thr Leu Phe Pro Thr Ala Ser Asp Glu Ala Val Leu
85 90 95
Arg Trp Leu Arg Asn Ala Gly Val Thr His Ile Ser Arg Gln Gly Gly
100 105 110
Leu Val Asn Phe Ala Thr Ser Val Gly Thr Val Asn Lys Leu Phe Asp
115 120 125
Thr Lys Phe Ser Tyr Tyr Lys Asn Gly Gln Val Gln Lys Leu Arg Thr
130 135 140
Thr Gln Tyr Ser Ile Pro Asp Asp Leu Ala Asp Gly Ile Asp Leu Val
145 150 155 160
Ala Pro Thr Val Phe Phe Gly Lys Gln Gln Glu Ser Val Ala Leu Pro
165 170 175
Thr His His Lys Phe Ala Ala Gly Thr Lys Thr Ala Ala Gly Asn Val
180 185 190
Ser Cys Ala Asp Ile Ile Thr Pro Asp Cys Leu Ala Asp Met Tyr Asn
195 200 205
Phe Arg Gly Tyr Lys Pro Ser Ala Ser Ser Gly Ser Lys Val Gly Phe
210 215 220
Gly Ser Phe Leu Asn Glu Ser Ala Asn Tyr Val Asp Leu Ala Leu Tyr
225 230 235 240
Glu Lys Arg Phe Asn Ile Pro Ser Gln Asn Phe Ser Val Glu Leu Ile
245 250 255
Asn Gly Ala Thr Asn Asp Gln Asn Trp Thr Thr Ala Ser Leu Gly Glu
260 265 270
Ala Asn Leu Asp Val Glu Leu Ile Val Gly Val Ser His Pro Leu Pro
275 280 285
Val Lys Glu Tyr Ile Thr Ala Gly Thr Pro Pro Phe Ile Pro Asn Ala
290 295 300
Leu Gln Pro Thr Pro Ala Asp Asn Gln Asn Glu Pro Tyr Leu Glu Tyr
305 310 315 320
Tyr Glu Tyr Leu Leu Ala Lys Pro Asn Ser Ala Leu Pro Gln Val Leu
325 330 335
Ser Asn Ser Tyr Gly Asp Glu Glu Gln Thr Val Pro Glu Tyr Tyr Ala
340 345 350
Lys Arg Val Cys Asn Leu Ile Gly Leu Met Gly Leu Arg Gly Ile Thr
355 360 365
Val Leu Glu Ser Ser Gly Asp Thr Gly Ile Gly Ala Ser Cys Met Ser
370 375 380
Asn Asp Gly Thr Glu Thr Pro Glu Phe Thr Pro Ile Phe Pro Ala Thr
385 390 395 400
Cys Pro Tyr Leu Thr Ser Val Gly Gly Thr Gln Ala Tyr Ala Pro Glu
405 410 415
Val Ala Trp Asp Ala Ser Ser Gly Gly Phe Ser Asn Tyr Phe Ser Arg
420 425 430
Ala Trp Tyr Gln Glu Ser Ala Val Ser Lys Tyr Leu Asp Gln Gln Ile
435 440 445
Thr Ala Glu Thr Lys Asp Tyr Tyr Ser Gln Tyr Thr Asn Phe Ser Gly
450 455 460
Arg Gly Phe Pro Asp Val Ala Ala His Ser Leu Thr Pro Arg Tyr Glu
465 470 475 480
Val Val Val Lys Gly Lys Arg Ala Thr Ser Gly Gly Thr Ser Ala Ala
485 490 495
Ala Pro Val Phe Ala Gly Leu Val Gly Met Leu Asn Asp Ala Arg Leu
500 505 510
Arg Ala Gly Lys Pro Thr Leu Gly Phe Leu Asn Pro Leu Leu Tyr Ser
515 520 525
Gly Ala Leu Lys Asp Phe Thr Asp Ile Thr Ala Gly Ser Ser Ile Gly
530 535 540
Cys Asp Gly Val Asn Pro Gln Thr Gly Lys Asn Val Thr Gly Gly Gly
545 550 555 560
Val Ile Pro Tyr Ala His Trp Asn Ala Thr Ala Gly Trp Asp Pro Val
565 570 575
Thr Gly Leu Gly Val Pro Asp Phe Met Lys Leu Lys Asp Leu Val Leu
580 585 590
Ser Leu
<210> 150
<211> 595
<212> PRT
<213> 黑曲霉
<400> 150
Met Arg Ser Ser Gly Leu Tyr Thr Ala Leu Leu Cys Ser Leu Ala Ala
1 5 10 15
Ser Thr Asn Ala Ile Val His Glu Lys Leu Ala Ala Val Pro Ser Gly
20 25 30
Trp His His Val Glu Asp Ala Gly Ser Asp His Gln Ile Ser Leu Ser
35 40 45
Ile Ala Leu Ala Arg Lys Asn Leu Asp Gln Leu Glu Ser Lys Leu Lys
50 55 60
Asp Leu Ser Thr Pro Gly Glu Ser Gln Tyr Gly Gln Trp Leu Asp Gln
65 70 75 80
Glu Asp Val Asp Thr Leu Phe Pro Val Ala Ser Asp Lys Ala Val Ile
85 90 95
Asn Trp Leu Arg Ser Ala Asn Ile Thr His Ile Ser Arg Gln Gly Ser
100 105 110
Leu Val Asn Phe Ala Thr Thr Val Asp Lys Val Asn Lys Leu Leu Asn
115 120 125
Ala Thr Phe Ala Tyr Tyr Gln Ser Gly Ser Ser Gln Arg Leu Arg Thr
130 135 140
Thr Glu Tyr Ser Ile Pro Asp Asp Leu Val Asp Ser Ile Asp Leu Ile
145 150 155 160
Ser Pro Thr Thr Phe Phe Gly Lys Glu Lys Thr Thr Ala Gly Leu Asn
165 170 175
Gln Arg Ala Gln Lys Ile Asp Thr His Val Ala Lys Arg Ser Asn Ser
180 185 190
Ser Ser Cys Ala Asp Val Ile Thr Leu Ser Cys Leu Lys Glu Met Tyr
195 200 205
Asn Phe Gly Asn Tyr Thr Pro Ser Ala Ser Ser Gly Ser Lys Leu Gly
210 215 220
Phe Gly Ser Phe Leu Asn Glu Ser Ala Ser Tyr Ser Asp Leu Ala Lys
225 230 235 240
Phe Glu Lys Leu Phe Asn Leu Pro Ser Gln Ser Phe Ser Val Glu Leu
245 250 255
Val Asn Gly Gly Val Asn Asp Gln Asn Gln Ser Thr Ala Ser Leu Thr
260 265 270
Glu Ala Asp Leu Asp Val Glu Leu Leu Val Gly Val Ala His Pro Leu
275 280 285
Pro Val Thr Glu Phe Ile Thr Ser Gly Glu Pro Pro Phe Ile Pro Asp
290 295 300
Pro Asp Glu Pro Ser Ala Ala Asp Asn Glu Asn Glu Pro Tyr Leu Gln
305 310 315 320
Tyr Tyr Glu Tyr Leu Leu Ser Lys Pro Asn Ser Ala Leu Pro Gln Val
325 330 335
Ile Ser Asn Ser Tyr Gly Asp Asp Glu Gln Thr Val Pro Glu Tyr Tyr
340 345 350
Ala Lys Arg Val Cys Asn Leu Ile Gly Leu Val Gly Leu Arg Gly Ile
355 360 365
Ser Val Leu Glu Ser Ser Gly Asp Glu Gly Ile Gly Ser Gly Cys Arg
370 375 380
Thr Thr Asp Gly Thr Asn Arg Thr Gln Phe Asn Pro Ile Phe Pro Ala
385 390 395 400
Thr Cys Pro Tyr Val Thr Ala Val Gly Gly Thr Met Ser Tyr Ala Pro
405 410 415
Glu Ile Ala Trp Glu Ala Ser Ser Gly Gly Phe Ser Asn Tyr Phe Glu
420 425 430
Arg Ala Trp Phe Gln Lys Glu Ala Val Gln Asn Tyr Leu Ala His His
435 440 445
Ile Thr Asn Glu Thr Lys Gln Tyr Tyr Ser Gln Phe Ala Asn Phe Ser
450 455 460
Gly Arg Gly Phe Pro Asp Val Ala Ala His Ser Phe Glu Pro Ser Tyr
465 470 475 480
Glu Val Ile Phe Tyr Gly Ala Arg Tyr Gly Ser Gly Gly Thr Ser Ala
485 490 495
Ala Cys Pro Leu Phe Ser Ala Leu Val Gly Met Leu Asn Asp Ala Arg
500 505 510
Leu Arg Ala Gly Lys Ser Thr Leu Gly Phe Leu Asn Pro Leu Leu Tyr
515 520 525
Ser Lys Gly Tyr Arg Ala Leu Thr Asp Val Thr Gly Gly Gln Ser Ile
530 535 540
Gly Cys Asn Gly Ile Asp Pro Gln Asn Asp Glu Thr Val Ala Gly Ala
545 550 555 560
Gly Ile Ile Pro Trp Ala His Trp Asn Ala Thr Val Gly Trp Asp Pro
565 570 575
Val Thr Gly Leu Gly Leu Pro Asp Phe Glu Lys Leu Arg Gln Leu Val
580 585 590
Leu Ser Leu
595
<210> 151
<211> 594
<212> PRT
<213> 棒曲霉
<400> 151
Met Leu Ser Ser Thr Leu Tyr Ala Gly Leu Leu Cys Ser Leu Ala Ala
1 5 10 15
Pro Ala Phe Gly Ile Ala His Glu Lys Leu Ala Ala Val Pro Asn Gly
20 25 30
Trp Thr Leu Ile Lys Asp Ala Pro Glu Thr Asp Thr Ile Thr Leu Ser
35 40 45
Ile Ala Leu Ala Arg Gln Asn Leu Asp Gln Leu Glu Ser Lys Leu Thr
50 55 60
Thr Leu Ala Thr Pro Gly Asn Ala Glu Tyr Gly Gln Trp Leu Asp Lys
65 70 75 80
Ala Asp Ile Glu Ser Leu Phe Pro Thr Ala Ser Asp Glu Ala Ile Ile
85 90 95
Glu Trp Leu Lys Ala Ala Gly Ile Thr Gln Val Ser Arg Gln Gly Ser
100 105 110
Leu Val Asn Phe Ala Ser Thr Val Gly Thr Val Asn Lys Leu Phe Asn
115 120 125
Thr Thr Phe Ser Tyr Tyr Gln Asn Gly Ala Ser Gln Lys Leu Arg Thr
130 135 140
Thr Glu Tyr Ser Ile Pro Asp Ser Leu Thr Asp Ala Ile Asp Leu Val
145 150 155 160
Ala Pro Thr Val Phe Phe Gly Lys Asp Gln Asp Asn Ala Leu Pro Ser
165 170 175
Phe Ala Ser Lys Leu Pro Thr Leu Pro Lys Arg Ala Ser Asn Ser Ser
180 185 190
Ser Cys Ala Asp Leu Ile Thr Pro Ala Cys Leu Ala Glu Met Tyr Asn
195 200 205
Leu Gly Asp Tyr Lys Pro Val Val Ser Ser Gly Ser Arg Ile Gly Phe
210 215 220
Gly Ser Phe Leu Asn Glu Ser Ala Asn Tyr Ala Asp Leu Ala Ala Tyr
225 230 235 240
Glu Lys Leu Phe Lys Ile Pro Ser Gln Asn Phe Ser Val Glu Leu Ile
245 250 255
Asn Gly Gly Thr Asn Asp Gln Asn Trp Asn Thr Ala Gln Leu Gly Glu
260 265 270
Ala Asp Leu Asp Val Glu Leu Ile Val Ala Val Ser His Pro Leu Pro
275 280 285
Val Val Glu Tyr Ile Thr Gly Gly Ser Pro Pro Tyr Val Pro Asn Ala
290 295 300
Asp Gly Pro Thr Ala Ala Asp Asn Gln Asn Glu Pro Tyr Leu Glu Tyr
305 310 315 320
Tyr Glu Tyr Leu Leu Ser Lys Pro Asn Ala Ala Leu Pro Gln Val Ile
325 330 335
Ser Asn Ser Tyr Gly Asp Asp Glu Gln Thr Val Pro Lys Tyr Tyr Ala
340 345 350
Lys Arg Val Cys Asn Met Ile Gly Leu Met Gly Leu Arg Gly Ile Thr
355 360 365
Val Leu Glu Ser Ser Gly Asp Thr Gly Ile Gly Ser Ala Cys Met Ser
370 375 380
Asn Asp Gly Thr Asn Thr Pro Gln Phe Thr Pro Thr Phe Pro Gly Thr
385 390 395 400
Cys Pro Phe Ile Thr Ser Val Gly Gly Thr Gln Ala Tyr Ala Pro Glu
405 410 415
Val Ala Trp Asp Gly Ser Ser Gly Gly Phe Ser Asn Tyr Phe Lys Arg
420 425 430
Pro Arg Tyr Gln Glu Ala Ala Val Lys Ser Tyr Leu Lys His His Ile
435 440 445
Ser Lys Asp Thr Lys Glu Tyr Tyr Ser Gln Tyr Thr Asn Phe His Gly
450 455 460
Arg Gly Phe Pro Asp Val Ser Ala His Ser Leu Thr Pro Asp Tyr Glu
465 470 475 480
Val Ile Asn Tyr Gly Ala Arg Ser Arg Ser Gly Gly Thr Ser Ala Ala
485 490 495
Cys Pro Val Phe Ala Gly Ile Val Gly Met Leu Asn Asp Ala Arg Leu
500 505 510
Arg Ala Gly Lys Pro Thr Leu Gly Phe Leu Asn Pro Leu Leu Tyr Ser
515 520 525
Glu Gly Phe Lys Thr Phe Thr Asp Ile Thr Ala Gly Ala Ser Val Gly
530 535 540
Cys Asp Gly Thr Asn Pro Gln Ser Gly Lys Pro Val Pro Gly Gly Gly
545 550 555 560
Ile Ile Pro Tyr Ala His Trp Asn Ala Thr Ala Gly Trp Asp Pro Val
565 570 575
Thr Gly Leu Gly Val Pro Asn Phe Leu Lys Leu Lys Glu Leu Val Leu
580 585 590
Ser Phe
<210> 152
<211> 580
<212> PRT
<213> 黑曲霉
<400> 152
Met Val Ala Phe Ser Arg Ile Ser Ala Gly Phe Ala Leu Ala Ala Pro
1 5 10 15
Ala Leu Ala Ser Val Val Leu Glu Thr Val Lys Ser Val Pro Ser Asp
20 25 30
Trp Lys Leu Val Glu Ala Ala Asp Thr Ser Ser Thr Ile Ser Leu Ser
35 40 45
Val Ala Leu Ala Arg Gln Asn Leu Asp Gln Leu Glu Glu Lys Leu Leu
50 55 60
Ala Val Ser Thr Pro Gly Lys Asp Thr Tyr Gly Gln Phe Leu Asp Leu
65 70 75 80
Asp Asp Ile Asn Glu Gln Phe Pro Leu Ala Asp Asp Ala Ala Val Val
85 90 95
Ala Trp Leu Lys Lys Ala Gly Val Thr Gln Ile His Lys Glu Gly Gly
100 105 110
Leu Leu Asn Phe Ala Thr Thr Val Gly Thr Ala Asn Gln Leu Leu Asn
115 120 125
Thr Thr Phe Ser Val Tyr Lys Ser Gly Ser Thr Gln Lys Leu Arg Thr
130 135 140
Thr Gln Tyr Ser Val Pro Asp Glu Leu Thr Gly Ser Ile Asp Leu Ile
145 150 155 160
Ser Pro Thr Val Phe Phe Gly Lys Ser Asn Ala Ala Arg Ser Ala Ala
165 170 175
Val Arg Ala Ser Gln Thr Thr Lys Glu Thr Ser Arg Lys Lys Ser Ser
180 185 190
Asn Val Cys Glu Tyr Ile Thr Pro Asp Cys Leu Lys Glu Gln Tyr Ser
195 200 205
Ile Asp Tyr Thr Pro Glu Ala Ser Ser Gly Ser Arg Val Gly Phe Gly
210 215 220
Ser Phe Leu Asn Glu Ser Ala Leu Tyr Ser Asp Leu Asp Leu Phe Thr
225 230 235 240
Gln Tyr Phe Asp Ile Pro Gln Gln Ser Phe Thr Val Glu Thr Ile Asn
245 250 255
Gly Gly Ile Asn Asn Gln Glu Asn Asp Pro Asp Gly Glu Ala Asp Leu
260 265 270
Asp Val Gln Asn Ile Val Gly Ile Ser His Pro Leu Pro Val Thr Glu
275 280 285
Tyr Ile Thr Gly Gly Ser Pro Pro Phe Ile Pro Asp Val Glu Thr Thr
290 295 300
Thr Asp Glu Asn Glu Pro Tyr Leu Gln Tyr Tyr Glu Tyr Leu Leu Ala
305 310 315 320
Lys Thr Asn Asp Glu Leu Pro Leu Val Ile Ser Asn Ser Tyr Gly Asp
325 330 335
Asp Glu Asp Thr Val Pro Ile Ala Tyr Ala Thr Arg Val Cys Asn Leu
340 345 350
Ile Gly Leu Met Gly Thr Arg Gly Ile Ser Ile Leu Glu Ser Ser Gly
355 360 365
Asp Ser Gly Val Gly Gly Ala Cys Met Ser Asn Asp Gly Thr Asp Lys
370 375 380
Thr Glu Phe Thr Pro Met Phe Pro Gly Thr Cys Pro Tyr Ile Thr Ala
385 390 395 400
Val Gly Gly Thr Gln Asp Val Pro Glu Val Ala Trp Val Asp Ser Ser
405 410 415
Gly Gly Phe Ser Asn Tyr Phe Ser Gln Pro Ser Tyr Gln Ser Asp Gln
420 425 430
Val Glu Thr Tyr Leu Asp Lys Tyr Ile Ser Ala Ser Thr Lys Lys Tyr
435 440 445
Tyr Glu Gln Tyr Thr Asn Phe Ser Gly Arg Ala Phe Pro Asp Val Ser
450 455 460
Ala Phe Ala Gly Ser Pro Tyr Tyr Glu Thr Tyr Ile Asp Gly Gln Leu
465 470 475 480
Gly Leu Val Ala Gly Thr Ser Gly Ala Ser Pro Val Phe Ala Gly Ile
485 490 495
Val Ala Leu Leu Asn Asp Ala Arg Leu Arg Ala Asn Lys Thr Ser Leu
500 505 510
Gly Phe Leu Asn Pro Trp Leu Tyr Ser Ser Gly Tyr Lys Ser Leu Asn
515 520 525
Asp Ile Thr Ser Gly Glu Ala Val Gly Cys Gln Gly Asp Val Glu Gly
530 535 540
Ala Gly Val Ile Pro Trp Ala Ser Trp Asn Ala Thr Thr Gly Trp Asp
545 550 555 560
Pro Ala Thr Gly Leu Gly Thr Pro Asn Phe Ala Lys Leu Lys Glu Ala
565 570 575
Val Leu Ala Leu
580
<210> 153
<211> 602
<212> PRT
<213> 棒曲霉
<400> 153
Met Phe Ser Ser Leu Phe Ser Arg Gly Ala Leu Ser Leu Ala Val Val
1 5 10 15
Ser Leu Leu Thr Ser Ser Val Ala Ala Glu Val Phe Glu Lys Leu Ser
20 25 30
Thr Val Pro Gln Gly Trp Lys Tyr Ser His Ala Pro Ser Asp Ser Asp
35 40 45
Pro Ile Arg Leu Gln Ile Ala Leu Gln Gln Gln Asp Val Asp Gly Phe
50 55 60
Glu Lys Ala Leu Leu Glu Met Ser Asp Pro Ser His Ala Ser Tyr Gly
65 70 75 80
Lys His Phe Gln Ser His Glu Glu Met Lys Arg Met Leu Leu Pro Ser
85 90 95
Gln Glu Ser Val Gln Ser Val Arg Ser Trp Leu Glu Ser Gly Gly Val
100 105 110
Thr Asp Ile Glu Glu Asp Ala Asp Trp Ile Asn Phe Arg Thr Thr Val
115 120 125
Gly Val Ala Asn Thr Leu Leu Asp Ala Asp Phe Lys Trp Tyr Val Ser
130 135 140
Asp Val Arg Ser Leu Glu Arg Leu Arg Thr Leu Ser Tyr Ser Ile Pro
145 150 155 160
Glu Ser Leu Ala Ser His Val His Met Ile Gln Pro Thr Thr Arg Phe
165 170 175
Gly Gln Ile Lys Ala Asn Arg Ala Thr Leu His Ser Ser Ser Leu Tyr
180 185 190
Arg Asp Glu Lys Ile Leu Ser Ala Ser Leu Gln Ala Gly Asn Ser Thr
195 200 205
Cys Asp Gln Val Ile Thr Pro Gln Cys Leu Lys Ser Leu Tyr Asn Ile
210 215 220
Gly Asp Tyr Lys Ala Asp Ala Arg Ser Gly Ser Lys Val Ala Phe Ala
225 230 235 240
Ser Phe Leu Glu Glu Tyr Ala Arg Tyr Asp Asp Leu Ala Lys Phe Glu
245 250 255
Glu Lys Leu Ala Pro Tyr Ala Ile Gly Gln Asn Phe Ser Val Ile Gln
260 265 270
Tyr Asn Gly Gly Leu Asn Asp Gln Asn Ser Thr Ser Asp Ser Gly Glu
275 280 285
Ala Asn Leu Asp Leu Gln Tyr Met Leu Gly Ile Ser Ala Pro Leu Pro
290 295 300
Ile Thr Glu Phe Ser Thr Gly Gly Arg Gly Lys Leu Ile Pro Asp Leu
305 310 315 320
Ser Ser Pro Asp Pro Asn Asp Asn Ser Asn Glu Pro Tyr Leu Glu Phe
325 330 335
Leu Gln Asn Val Leu Lys Met Pro Gln His Lys Leu Pro Gln Val Ile
340 345 350
Ser Thr Ser Tyr Gly Glu Asp Glu Gln Thr Ile Pro Lys Arg Tyr Ala
355 360 365
Leu Ser Val Cys Asn Leu Tyr Ala Gln Leu Gly Ser Arg Gly Val Ser
370 375 380
Val Ile Phe Ser Ser Gly Asp Ser Gly Val Gly Ala Ala Cys Gln Thr
385 390 395 400
Asn Asp Gly His Asn Thr Thr His Phe Pro Pro Gln Phe Pro Ala Ala
405 410 415
Cys Pro Trp Val Thr Ser Val Gly Gly Thr Thr Asn Thr Gln Pro Glu
420 425 430
Glu Ala Val Tyr Phe Ser Ser Gly Gly Phe Ser Asp Leu Trp Pro Arg
435 440 445
Pro Gly Tyr Gln Glu Val Ala Val Arg Gly Tyr Leu Lys Lys Leu Gly
450 455 460
Gly Thr Tyr Arg Gly Leu Tyr Asn Pro Arg Gly Arg Ala Phe Pro Asp
465 470 475 480
Val Ala Ala Gln Ala Met Lys Tyr Ala Val Phe Asp Lys Gly Ala Leu
485 490 495
His Gln Tyr Ala Gly Thr Ser Cys Ser Ala Pro Ala Phe Gly Gly Ile
500 505 510
Ile Gly Leu Leu Asn Asp Ala Arg Leu Arg Ala His Lys Pro Pro Met
515 520 525
Gly Phe Leu Asn Pro Trp Leu Tyr Ser Gln Ala Ser His Gly Phe Asn
530 535 540
Asp Ile Val Val Gly Gly Ser Lys Gly Cys Asp Gly Arg Asn Arg Phe
545 550 555 560
Gly Gly Thr Pro Asn Gly Ser Pro Val Val Pro Tyr Ala Ser Trp Asn
565 570 575
Ala Thr Glu Gly Trp Asp Pro Ala Thr Gly Leu Gly Thr Pro Asn Phe
580 585 590
Gly Lys Leu Leu His Leu Ala Leu Arg Arg
595 600
<210> 154
<211> 600
<212> PRT
<213> 土曲霉
<400> 154
Met Leu Pro Ser Leu Val Asn Asn Gly Ala Leu Ser Leu Ala Val Leu
1 5 10 15
Ser Leu Leu Thr Ser Ser Val Ala Gly Glu Val Phe Glu Lys Leu Ser
20 25 30
Ala Val Pro Lys Gly Trp His Phe Ser His Ala Ala Gln Ala Asp Ala
35 40 45
Pro Ile Asn Leu Lys Ile Ala Leu Lys Gln His Asp Val Glu Gly Phe
50 55 60
Glu Gln Ala Leu Leu Asp Met Ser Thr Pro Gly His Glu Asn Tyr Gly
65 70 75 80
Lys His Phe His Glu His Asp Glu Met Lys Arg Met Leu Leu Pro Ser
85 90 95
Asp Ser Ala Val Asp Ala Val Gln Thr Trp Leu Thr Ser Ala Gly Ile
100 105 110
Thr Asp Tyr Asp Leu Asp Ala Asp Trp Ile Asn Leu Arg Thr Thr Val
115 120 125
Glu His Ala Asn Ala Leu Leu Asp Thr Gln Phe Gly Trp Tyr Glu Asn
130 135 140
Glu Val Arg His Ile Thr Arg Leu Arg Thr Leu Gln Tyr Ser Ile Pro
145 150 155 160
Glu Thr Val Ala Ala His Ile Asn Met Val Gln Pro Thr Thr Arg Phe
165 170 175
Gly Gln Ile Arg Pro Asp Arg Ala Thr Phe His Ala His His Thr Ser
180 185 190
Asp Ala Arg Ile Leu Ser Ala Leu Ala Ala Ala Ser Asn Ser Thr Ser
195 200 205
Cys Asp Ser Val Ile Thr Pro Lys Cys Leu Lys Asp Leu Tyr Lys Val
210 215 220
Gly Asp Tyr Glu Ala Asp Pro Asp Ser Gly Ser Gln Val Ala Phe Ala
225 230 235 240
Ser Tyr Leu Glu Glu Tyr Ala Arg Tyr Ala Asp Met Val Lys Phe Gln
245 250 255
Asn Ser Leu Ala Pro Tyr Ala Lys Gly Gln Asn Phe Ser Val Val Leu
260 265 270
Tyr Asn Gly Gly Val Asn Asp Gln Ser Ser Ser Ala Asp Ser Gly Glu
275 280 285
Ala Asn Leu Asp Leu Gln Thr Ile Met Gly Leu Ser Ala Pro Leu Pro
290 295 300
Ile Thr Glu Tyr Ile Thr Gly Gly Arg Gly Lys Leu Ile Pro Asp Leu
305 310 315 320
Ser Gln Pro Asn Pro Asn Asp Asn Ser Asn Glu Pro Tyr Leu Glu Phe
325 330 335
Leu Gln Asn Ile Leu Lys Leu Asp Gln Asp Glu Leu Pro Gln Val Ile
340 345 350
Ser Thr Ser Tyr Gly Glu Asp Glu Gln Thr Ile Pro Arg Gly Tyr Ala
355 360 365
Glu Ser Val Cys Asn Met Leu Ala Gln Leu Gly Ser Arg Gly Val Ser
370 375 380
Val Val Phe Ser Ser Gly Asp Ser Gly Val Gly Ala Ala Cys Gln Thr
385 390 395 400
Asn Asp Gly Arg Asn Gln Thr His Phe Asn Pro Gln Phe Pro Ala Ser
405 410 415
Cys Pro Trp Val Thr Ser Val Gly Ala Thr Thr Lys Thr Asn Pro Glu
420 425 430
Gln Ala Val Tyr Phe Ser Ser Gly Gly Phe Ser Asp Phe Trp Lys Arg
435 440 445
Pro Lys Tyr Gln Asp Glu Ala Val Ala Ala Tyr Leu Asp Thr Leu Gly
450 455 460
Asp Lys Phe Ala Gly Leu Phe Asn Lys Gly Gly Arg Ala Phe Pro Asp
465 470 475 480
Val Ala Ala Gln Gly Met Asn Tyr Ala Ile Tyr Asp Lys Gly Thr Leu
485 490 495
Gly Arg Leu Asp Gly Thr Ser Cys Ser Ala Pro Ala Phe Ser Ala Ile
500 505 510
Ile Ser Leu Leu Asn Asp Ala Arg Leu Arg Glu Gly Lys Pro Thr Met
515 520 525
Gly Phe Leu Asn Pro Trp Leu Tyr Gly Glu Gly Arg Glu Ala Leu Asn
530 535 540
Asp Val Val Val Gly Gly Ser Lys Gly Cys Asp Gly Arg Asp Arg Phe
545 550 555 560
Gly Gly Lys Pro Asn Gly Ser Pro Val Val Pro Phe Ala Ser Trp Asn
565 570 575
Ala Thr Gln Gly Trp Asp Pro Val Thr Gly Leu Gly Thr Pro Asn Phe
580 585 590
Ala Lys Met Leu Glu Leu Ala Pro
595 600
<210> 155
<211> 602
<212> PRT
<213> 烟曲霉
<400> 155
Met Phe Ser Ser Leu Leu Asn Arg Gly Ala Leu Leu Ala Val Val Ser
1 5 10 15
Leu Leu Ser Ser Ser Val Ala Ala Glu Val Phe Glu Lys Leu Ser Ala
20 25 30
Val Pro Gln Gly Trp Lys Tyr Ser His Thr Pro Ser Asp Arg Asp Pro
35 40 45
Ile Arg Leu Gln Ile Ala Leu Lys Gln His Asp Val Glu Gly Phe Glu
50 55 60
Thr Ala Leu Leu Glu Met Ser Asp Pro Tyr His Pro Asn Tyr Gly Lys
65 70 75 80
His Phe Gln Thr His Glu Glu Met Lys Arg Met Leu Leu Pro Thr Gln
85 90 95
Glu Ala Val Glu Ser Val Arg Gly Trp Leu Glu Ser Ala Gly Ile Ser
100 105 110
Asp Ile Glu Glu Asp Ala Asp Trp Ile Lys Phe Arg Thr Thr Val Gly
115 120 125
Val Ala Asn Asp Leu Leu Asp Ala Asp Phe Lys Trp Tyr Val Asn Glu
130 135 140
Val Gly His Val Glu Arg Leu Arg Thr Leu Ala Tyr Ser Leu Pro Gln
145 150 155 160
Ser Val Ala Ser His Val Asn Met Val Gln Pro Thr Thr Arg Phe Gly
165 170 175
Gln Ile Lys Pro Asn Arg Ala Thr Met Arg Gly Arg Pro Val Gln Val
180 185 190
Asp Ala Asp Ile Leu Ser Ala Ala Val Gln Ala Gly Asp Thr Ser Thr
195 200 205
Cys Asp Gln Val Ile Thr Pro Gln Cys Leu Lys Asp Leu Tyr Asn Ile
210 215 220
Gly Asp Tyr Lys Ala Asp Pro Asn Gly Gly Ser Lys Val Ala Phe Ala
225 230 235 240
Ser Phe Leu Glu Glu Tyr Ala Arg Tyr Asp Asp Leu Ala Lys Phe Glu
245 250 255
Glu Lys Leu Ala Pro Tyr Ala Ile Gly Gln Asn Phe Ser Val Ile Gln
260 265 270
Tyr Asn Gly Gly Leu Asn Asp Gln Asn Ser Ala Ser Asp Ser Gly Glu
275 280 285
Ala Asn Leu Asp Leu Gln Tyr Ile Val Gly Val Ser Ser Pro Ile Pro
290 295 300
Val Thr Glu Phe Ser Thr Gly Gly Arg Gly Leu Leu Ile Pro Asp Leu
305 310 315 320
Ser Gln Pro Asp Pro Asn Asp Asn Ser Asn Glu Pro Tyr Leu Glu Phe
325 330 335
Leu Gln Asn Val Leu Lys Met Asp Gln Asp Lys Leu Pro Gln Val Ile
340 345 350
Ser Thr Ser Tyr Gly Glu Asp Glu Gln Thr Ile Pro Glu Lys Tyr Ala
355 360 365
Arg Ser Val Cys Asn Leu Tyr Ala Gln Leu Gly Ser Arg Gly Val Ser
370 375 380
Val Ile Phe Ser Ser Gly Asp Ser Gly Val Gly Ala Ala Cys Leu Thr
385 390 395 400
Asn Asp Gly Thr Asn Arg Thr His Phe Pro Pro Gln Phe Pro Ala Ala
405 410 415
Cys Pro Trp Val Thr Ser Val Gly Gly Thr Thr Lys Thr Gln Pro Glu
420 425 430
Glu Ala Val Tyr Phe Ser Ser Gly Gly Phe Ser Asp Leu Trp Glu Arg
435 440 445
Pro Ser Trp Gln Asp Ser Ala Val Lys Arg Tyr Leu Lys Lys Leu Gly
450 455 460
Pro Arg Tyr Lys Gly Leu Tyr Asn Pro Lys Gly Arg Ala Phe Pro Asp
465 470 475 480
Val Ala Ala Gln Ala Glu Asn Tyr Ala Val Phe Asp Lys Gly Val Leu
485 490 495
His Gln Phe Asp Gly Thr Ser Cys Ser Ala Pro Ala Phe Ser Ala Ile
500 505 510
Val Ala Leu Leu Asn Asp Ala Arg Leu Arg Ala His Lys Pro Val Met
515 520 525
Gly Phe Leu Asn Pro Trp Leu Tyr Ser Lys Ala Ser Lys Gly Phe Asn
530 535 540
Asp Ile Val Lys Gly Gly Ser Lys Gly Cys Asp Gly Arg Asn Arg Phe
545 550 555 560
Gly Gly Thr Pro Asn Gly Ser Pro Val Val Pro Tyr Ala Ser Trp Asn
565 570 575
Ala Thr Asp Gly Trp Asp Pro Ala Thr Gly Leu Gly Thr Pro Asp Phe
580 585 590
Gly Lys Leu Leu Ser Leu Ala Met Arg Arg
595 600
<210> 156
<211> 595
<212> PRT
<213> 核盘菌
<400> 156
Met Met Tyr Pro Lys Thr Ala Ile Cys Ala Gly Leu Leu Ser Gln Ala
1 5 10 15
Leu Pro Ala Tyr Gly Ala Val Met Glu Lys Leu Ala Ala Val Pro Ser
20 25 30
Gly Trp Thr Val Ala Glu Thr Asp Ser Ser Thr Ala Ile Thr Leu Thr
35 40 45
Val Gly Leu Ala Met Gln Asn Leu Asp Gln Leu Glu Ala Lys Leu Ile
50 55 60
Ala Val Ser Thr Pro Gly Asn Ala Asp Tyr Gly Asn His Leu Asp Ala
65 70 75 80
Asp Glu Ala Asn Ala Leu Phe Ala Pro Ser Ala Asp Ala Ser Asn Ala
85 90 95
Val Thr Ser Trp Leu Thr Ser Ala Gly Ala Gln Asn Ile Val Ser Asp
100 105 110
Gly Ser Thr Val Thr Phe Ala Ala Thr Ile Glu Lys Ala Asn Ser Ile
115 120 125
Leu Asn Ala Thr Phe Gln Thr Tyr Thr Ser Asn Gly Val Thr Lys Val
130 135 140
Arg Thr Thr Gln Tyr Ser Val Pro Asp His Leu Ala Glu His Ile Asp
145 150 155 160
Leu Ile Ser Pro Thr Thr Tyr Phe Gly Lys Thr Val Ala Asn Ala Pro
165 170 175
Thr Lys Val Thr Lys Lys Asp Thr Ser Ala Thr Lys Val Thr Val Asp
180 185 190
Ala Ser Cys Ser Thr Ser Ile Thr Pro Lys Cys Ile Lys Glu Leu Tyr
195 200 205
Asn Val Gly Asp Tyr Thr Pro Ser Val Ser Ser Gly Ser Arg Ile Gly
210 215 220
Phe Gly Ser Phe Leu Asn Gln Ser Ala Leu Tyr Ser Asp Leu Phe Gln
225 230 235 240
Tyr Glu Lys Tyr Phe Asp Ile Pro Gln Gln Asn Phe Ser Val Thr Leu
245 250 255
Ile Asn Gly Ala Thr Asn Asp Gln Asn Pro Ala Thr Ala Gln Asn Gly
260 265 270
Glu Ala Asn Leu Asp Val Gln Asn Ile Ile Gly Val Ala His Pro Leu
275 280 285
Pro Val Gln Glu Phe Ile Thr Gly Gly Ser Pro Pro Phe Val Pro Asn
290 295 300
Ile Asp Val Pro Thr Ala Ala Asp Asn Ser Asn Glu Pro Tyr Leu Pro
305 310 315 320
Tyr Tyr Gln Tyr Leu Met Thr Gln Pro Asn Ser Ala Ile Pro Gln Val
325 330 335
Ile Ser Asn Ser Tyr Gly Asp Asp Glu Asp Thr Val Pro Tyr Ala Tyr
340 345 350
Ala Val Arg Val Cys Asn Leu Ile Gly Ile Met Gly Leu Arg Gly Ile
355 360 365
Ser Ile Leu Glu Ser Ser Gly Asp Thr Gly Val Gly Ala Gly Cys Val
370 375 380
Ala Asn Asp Gly Lys Asp Thr Pro Tyr Phe Gln Pro Gln Phe Pro Gly
385 390 395 400
Thr Cys Pro Tyr Ile Thr Ser Val Gly Gly Thr Gln Ser Val Ser Pro
405 410 415
Glu Val Ala Trp Asp Asp Gly Ser Gly Gly Phe Ser Asn Tyr Phe Pro
420 425 430
Thr Ala Trp Tyr Gln Thr Ala Ala Val Ala Glu Tyr Leu Thr Glu His
435 440 445
Ile Thr Pro Ser Thr Arg Lys Tyr Tyr Glu Ser Tyr Thr Asn Phe Ser
450 455 460
Gly Arg Gly Phe Pro Asp Ile Ser Ala His Ser Leu Thr Pro Asp Tyr
465 470 475 480
Gln Val Val Tyr Ser Gly Glu Leu Ser Pro Ser Gly Gly Thr Ser Ala
485 490 495
Ala Ala Pro Val Val Ala Ala Ile Leu Gly Leu Leu Asn Asp Ala Arg
500 505 510
Phe Lys Ala Gly Lys Ser Ala Leu Gly Phe Val Asn Pro Leu Leu Tyr
515 520 525
Leu Leu Gly Thr Ser Ala Leu Asn Asp Ile Thr Gly Gly Gly Ser Val
530 535 540
Gly Cys Asn Gly Val Asn Gly Gln Thr Gly Ala Lys Ile Pro Asn Gly
545 550 555 560
Ala Val Ile Pro Tyr Ala Thr Trp Asn Ala Thr Lys Gly Trp Asp Pro
565 570 575
Val Thr Gly Leu Gly Thr Pro Asp Phe Glu Lys Leu Lys Glu Leu Val
580 585 590
Leu Ser Tyr
595
<210> 157
<211> 595
<212> PRT
<213> 灰葡萄孢霉
<400> 157
Met Tyr Pro Lys Thr Ala Ile Cys Ala Gly Leu Leu Ser Gln Ala Leu
1 5 10 15
Pro Ala Tyr Gly Ala Val Met Glu Lys Leu Ala Ala Ala Pro Ser Gly
20 25 30
Trp Thr Ala Ala Ser Glu Thr Asp Ser Ser Ala Ala Ile Thr Leu Thr
35 40 45
Val Gly Leu Ala Met Gln Asn Leu Asp Gln Leu Glu Ala Lys Leu Leu
50 55 60
Ala Val Ser Thr Pro Gly Asn Ala Glu Tyr Gly Asn His Leu Asp Ile
65 70 75 80
Asp Glu Ala Asn Ala Leu Phe Ala Pro Ser Ala Asp Ala Ser Ser Ala
85 90 95
Val Thr Ser Trp Leu Thr Ser Ala Gly Ala Gln Asn Ile Val Ser Asp
100 105 110
Gly Ser Thr Ile Ser Phe Ala Thr Thr Ile Glu Lys Ala Asn Ser Met
115 120 125
Leu Asn Ala Lys Phe Lys Ser Tyr Thr Ser Asn Gly Val Thr Lys Val
130 135 140
Arg Thr Thr Gln Tyr Ser Val Pro Asp Asp Val Ala Glu His Ile Glu
145 150 155 160
Leu Ile Ser Pro Thr Thr Tyr Phe Gly Lys Thr Val Ala Asn Ala Pro
165 170 175
Thr Lys Ile Ala Lys Arg Asp Thr Ser Ala Thr Lys Val Thr Val Asp
180 185 190
Ala Ala Cys Ser Thr Ser Ile Thr Pro Lys Cys Ile Lys Glu Leu Tyr
195 200 205
Asn Ile Gly Asp Tyr Thr Pro Ser Val Ser Ser Gly Ser Arg Ile Gly
210 215 220
Phe Gly Ser Phe Leu Asn Gln Ser Ala Leu Tyr Thr Asp Leu Phe Gln
225 230 235 240
Tyr Glu Ser Tyr Phe Gly Ile Pro Glu Gln Asn Phe Thr Val Thr Leu
245 250 255
Ile Asn Gly Ala Thr Asn Asp Gln Asn Glu Ala Thr Ala Gln Ile Gly
260 265 270
Glu Ala Asn Leu Asp Val Gln Asn Ile Ile Gly Val Ala His Pro Leu
275 280 285
Pro Val Gln Glu Phe Ile Thr Gly Gly Ser Pro Pro Phe Ile Pro Asn
290 295 300
Val Asp Leu Pro Thr Glu Ala Asp Asn Ser Asn Glu Pro Tyr Leu Pro
305 310 315 320
Tyr Tyr Glu Tyr Leu Leu Ser Gln Pro Asn Ser Ala Leu Pro Gln Val
325 330 335
Ile Ser Asn Ser Tyr Gly Asp Asp Glu Asp Thr Val Pro Tyr Ala Tyr
340 345 350
Ala Val Arg Val Cys Asn Leu Ile Gly Ile Met Gly Leu Arg Gly Ile
355 360 365
Ser Val Leu Glu Ser Ser Gly Asp Thr Gly Val Gly Ala Gly Cys Val
370 375 380
Ala Asn Asp Gly Leu Asn Thr Pro Tyr Phe Gln Pro Gln Phe Pro Gly
385 390 395 400
Thr Cys Pro Tyr Ile Thr Ser Val Gly Gly Thr Gln Ala Val Ala Pro
405 410 415
Glu Val Ala Trp Asp Asp Gly Ser Gly Gly Phe Ser Asn Tyr Phe Pro
420 425 430
Thr Ala Trp Tyr Gln Val Ala Ala Val Asp Glu Tyr Leu Asn Lys His
435 440 445
Ile Ser Pro Ser Thr Lys Glu Tyr Tyr Glu Ser Tyr Thr Asn Phe Ser
450 455 460
Gly Arg Gly Phe Pro Asp Ile Ser Ala His Ser Leu Thr Pro Asp Tyr
465 470 475 480
Gln Val Val Tyr Phe Gly Glu Leu Ser Pro Ser Gly Gly Thr Ser Ala
485 490 495
Ala Ala Pro Val Ile Ala Gly Ile Leu Gly Leu Leu Asn Asp Ala Arg
500 505 510
Phe Lys Ala Gly Lys Pro Ala Leu Gly Phe Ile Asn Pro Leu Leu Tyr
515 520 525
Leu Leu Gly Gln Gly Ala Leu Asn Asp Ile Thr Gly Gly Ala Ser Val
530 535 540
Gly Cys Asn Gly Val Asn Gly Gln Thr Gly Ala Lys Val Pro Gly Gly
545 550 555 560
Ala Val Ile Pro Tyr Ala Ser Trp Asn Ala Thr Lys Gly Trp Asp Pro
565 570 575
Val Thr Gly Leu Gly Thr Pro Asn Phe Glu Lys Leu Lys Ala Leu Val
580 585 590
Leu Ser Tyr
595
<210> 158
<211> 594
<212> PRT
<213> 费希新萨托菌
<400> 158
Met Leu Ser Ser Thr Leu Tyr Ala Gly Leu Leu Cys Ser Leu Ala Ala
1 5 10 15
Pro Ala Leu Gly Val Val His Glu Lys Leu Ser Ala Val Pro Ser Gly
20 25 30
Trp Thr Leu Val Glu Asp Ala Ser Glu Ser Asp Thr Thr Thr Leu Ser
35 40 45
Ile Ala Leu Ala Arg Gln Asn Leu Asp Gln Leu Glu Ser Lys Leu Thr
50 55 60
Thr Leu Ala Thr Pro Gly Asn Ala Glu Tyr Gly Lys Trp Leu Asp Gln
65 70 75 80
Ser Asp Ile Glu Ser Leu Phe Pro Thr Ala Ser Asp Asp Ala Val Ile
85 90 95
Gln Trp Leu Lys Asp Ala Gly Val Thr Gln Val Ser Arg Gln Gly Ser
100 105 110
Leu Val Asn Phe Ala Thr Thr Val Gly Thr Ala Asn Lys Leu Phe Asp
115 120 125
Thr Lys Phe Ser Tyr Tyr Arg Asn Gly Ala Ser Gln Lys Leu Arg Thr
130 135 140
Thr Gln Tyr Ser Ile Pro Asp Ser Leu Thr Glu Ser Ile Asp Leu Ile
145 150 155 160
Ala Pro Thr Val Phe Phe Gly Lys Glu Gln Asp Ser Ala Leu Pro Pro
165 170 175
His Ala Val Lys Leu Pro Ala Leu Pro Arg Arg Ala Ala Thr Asn Ser
180 185 190
Ser Cys Ala Asn Leu Ile Thr Pro Asp Cys Leu Val Glu Met Tyr Asn
195 200 205
Leu Gly Asp Tyr Lys Pro Asp Ala Ser Ser Gly Ser Arg Val Gly Phe
210 215 220
Gly Ser Phe Leu Asn Gln Ser Ala Asn Tyr Ala Asp Leu Ala Ala Tyr
225 230 235 240
Glu Gln Leu Phe Asn Ile Pro Pro Gln Asn Phe Ser Val Glu Leu Ile
245 250 255
Asn Gly Gly Ala Asn Asp Gln Asn Trp Ala Thr Ala Ser Leu Gly Glu
260 265 270
Ala Asn Leu Asp Val Glu Leu Ile Val Ala Val Ser His Ala Leu Pro
275 280 285
Val Val Glu Phe Ile Thr Gly Gly Ser Pro Pro Phe Val Pro Asn Val
290 295 300
Asp Glu Pro Thr Ala Ala Asp Asn Gln Asn Glu Pro Tyr Leu Gln Tyr
305 310 315 320
Tyr Glu Tyr Leu Leu Ser Lys Pro Asn Ser His Leu Pro Gln Val Ile
325 330 335
Ser Asn Ser Tyr Gly Asp Asp Glu Gln Thr Val Pro Glu Tyr Tyr Ala
340 345 350
Arg Arg Val Cys Asn Leu Ile Gly Leu Met Gly Leu Arg Gly Ile Thr
355 360 365
Val Leu Glu Ser Ser Gly Asp Thr Gly Ile Gly Ser Ala Cys Met Ser
370 375 380
Asn Asp Gly Thr Asn Thr Pro Gln Phe Thr Pro Thr Phe Pro Gly Thr
385 390 395 400
Cys Pro Phe Ile Thr Ala Val Gly Gly Thr Gln Ser Tyr Ala Pro Glu
405 410 415
Val Ala Trp Asp Ala Ser Ser Gly Gly Phe Ser Asn Tyr Phe Ser Arg
420 425 430
Pro Trp Tyr Gln Tyr Phe Ala Val Glu Asn Tyr Leu Asn Asn His Ile
435 440 445
Thr Lys Asp Thr Lys Lys Tyr Tyr Ser Gln Tyr Thr Asn Phe Lys Gly
450 455 460
Arg Gly Phe Pro Asp Val Ser Ala His Ser Leu Thr Pro Asp Tyr Glu
465 470 475 480
Val Val Leu Thr Gly Lys His Tyr Lys Ser Gly Gly Thr Ser Ala Ala
485 490 495
Cys Pro Val Phe Ala Gly Ile Val Gly Leu Leu Asn Asp Ala Arg Leu
500 505 510
Arg Ala Gly Lys Ser Thr Leu Gly Phe Leu Asn Pro Leu Leu Tyr Ser
515 520 525
Ile Leu Ala Glu Gly Phe Thr Asp Ile Thr Ala Gly Ser Ser Ile Gly
530 535 540
Cys Asn Gly Ile Asn Pro Gln Thr Gly Lys Pro Val Pro Gly Gly Gly
545 550 555 560
Ile Ile Pro Tyr Ala His Trp Asn Ala Thr Ala Gly Trp Asp Pro Val
565 570 575
Thr Gly Leu Gly Val Pro Asp Phe Met Lys Leu Lys Glu Leu Val Leu
580 585 590
Ser Leu
<210> 159
<211> 605
<212> PRT
<213> 柄篮状菌
<400> 159
Met Ser Arg Asn Leu Leu Val Gly Ala Gly Leu Leu Ala Leu Ala Gln
1 5 10 15
Leu Ser Gly Gln Ala Leu Ala Ala Ala Ala Leu Val Gly His Glu Ser
20 25 30
Leu Ala Ala Leu Pro Val Gly Trp Asp Lys Val Ser Thr Pro Ala Ala
35 40 45
Gly Thr Asn Ile Gln Leu Ser Val Ala Leu Ala Leu Gln Asn Ile Glu
50 55 60
Gln Leu Glu Asp His Leu Lys Ser Val Ser Thr Pro Gly Ser Ala Ser
65 70 75 80
Tyr Gly Gln Tyr Leu Asp Ser Asp Gly Ile Ala Ala Gln Tyr Gly Pro
85 90 95
Ser Asp Ala Ser Val Glu Ala Val Thr Asn Trp Leu Lys Glu Ala Gly
100 105 110
Val Thr Asp Ile Tyr Asn Asn Gly Gln Ser Ile His Phe Ala Thr Ser
115 120 125
Val Ser Lys Ala Asn Ser Leu Leu Gly Ala Asp Phe Asn Tyr Tyr Ser
130 135 140
Asp Gly Ser Ala Thr Lys Leu Arg Thr Leu Ala Tyr Ser Val Pro Ser
145 150 155 160
Asp Leu Lys Glu Ala Ile Asp Leu Val Ser Pro Thr Thr Tyr Phe Gly
165 170 175
Lys Thr Thr Ala Ser Arg Ser Ile Gln Ala Tyr Lys Asn Lys Arg Ala
180 185 190
Ser Thr Thr Ser Lys Ser Gly Ser Ser Ser Val Gln Val Ser Ala Ser
195 200 205
Cys Gln Thr Ser Ile Thr Pro Ala Cys Leu Lys Gln Met Tyr Asn Val
210 215 220
Gly Asn Tyr Thr Pro Ser Val Ala His Gly Ser Arg Val Gly Phe Gly
225 230 235 240
Ser Phe Leu Asn Gln Ser Ala Ile Phe Asp Asp Leu Phe Thr Tyr Glu
245 250 255
Lys Val Asn Asp Ile Pro Ser Gln Asn Phe Thr Lys Val Ile Ile Ala
260 265 270
Asn Ala Ser Asn Ser Gln Asp Ala Ser Asp Gly Asn Tyr Gly Glu Ala
275 280 285
Asn Leu Asp Val Gln Asn Ile Val Gly Ile Ser His Pro Leu Pro Val
290 295 300
Thr Glu Phe Leu Thr Gly Gly Ser Pro Pro Phe Val Ala Ser Leu Asp
305 310 315 320
Thr Pro Thr Asn Gln Asn Glu Pro Tyr Ile Pro Tyr Tyr Glu Tyr Leu
325 330 335
Leu Ser Gln Lys Asn Glu Asp Leu Pro Gln Val Ile Ser Asn Ser Tyr
340 345 350
Gly Asp Asp Glu Gln Ser Val Pro Tyr Lys Tyr Ala Ile Arg Ala Cys
355 360 365
Asn Leu Ile Gly Leu Thr Gly Leu Arg Gly Ile Ser Val Leu Glu Ser
370 375 380
Ser Gly Asp Leu Gly Val Gly Ala Gly Cys Arg Ser Asn Asp Gly Lys
385 390 395 400
Asn Lys Thr Gln Phe Asp Pro Ile Phe Pro Ala Thr Cys Pro Tyr Val
405 410 415
Thr Ser Val Gly Gly Thr Gln Ser Val Thr Pro Glu Ile Ala Trp Val
420 425 430
Ala Ser Ser Gly Gly Phe Ser Asn Tyr Phe Pro Arg Thr Trp Tyr Gln
435 440 445
Glu Pro Ala Ile Gln Thr Tyr Leu Gly Leu Leu Asp Asp Glu Thr Lys
450 455 460
Thr Tyr Tyr Ser Gln Tyr Thr Asn Phe Glu Gly Arg Gly Phe Pro Asp
465 470 475 480
Val Ser Ala His Ser Leu Thr Pro Asp Tyr Gln Val Val Gly Gly Gly
485 490 495
Tyr Leu Gln Pro Ser Gly Gly Thr Ser Ala Ala Ser Pro Val Phe Ala
500 505 510
Gly Ile Ile Ala Leu Leu Asn Asp Ala Arg Leu Ala Ala Gly Lys Pro
515 520 525
Thr Leu Gly Phe Leu Asn Pro Phe Phe Tyr Leu Tyr Gly Tyr Lys Gly
530 535 540
Leu Asn Asp Ile Thr Gly Gly Gln Ser Val Gly Cys Asn Gly Ile Asn
545 550 555 560
Gly Gln Thr Gly Ala Pro Val Pro Gly Gly Gly Ile Val Pro Gly Ala
565 570 575
Ala Trp Asn Ser Thr Thr Gly Trp Asp Pro Ala Thr Gly Leu Gly Thr
580 585 590
Pro Asp Phe Gln Lys Leu Lys Glu Leu Val Leu Ser Phe
595 600 605
<210> 160
<211> 604
<212> PRT
<213> 马尼弗青霉菌
<400> 160
Met Ser Arg Asn Leu Leu Val Gly Ala Gly Leu Leu Ala Leu Ala Gln
1 5 10 15
Leu Asn Pro Val Leu Ala Ala Ser Ala Leu Val Ser His Glu Ala Leu
20 25 30
Ala Thr Leu Pro Pro Gly Trp Asp Lys Val Asn Thr Pro Ala Gly His
35 40 45
Thr Asn Ile Gln Leu Ser Val Ala Leu Thr Leu Gln Asn Ile Glu Gln
50 55 60
Leu Gln Ser His Leu Gln Ser Val Ser Thr Pro Gly Ser Ser Gly Tyr
65 70 75 80
Gly Gln Tyr Leu Asp Ser Asp Asp Ile Ala Ala Gln Tyr Gly Pro Ser
85 90 95
Asp Ala Ser Val Asp Ala Val Thr Lys Trp Leu Lys Glu Ala Gly Ile
100 105 110
Thr Glu Ile His Asn Asn Gly Gln Ser Ile Asn Phe Ala Thr Thr Val
115 120 125
Ser Lys Ala Asn Lys Leu Leu Gly Ala Asp Phe His Tyr Tyr Ser Asp
130 135 140
Gly Gly Ala Thr Lys Leu Arg Thr Leu Ala Tyr Ser Val Pro Ser Asp
145 150 155 160
Leu Gln Ala Ala Ile Asp Leu Val Ser Pro Thr Thr Tyr Phe Gly Lys
165 170 175
Thr Thr Ala Ser Arg Ser Ile Gln Thr Tyr Ser Asn Lys Arg Ala Ser
180 185 190
Thr Thr Thr Lys Ser Gly Ser Ser Ser Val Ala Val Ala Ala Ser Cys
195 200 205
Gln Thr Ser Ile Thr Pro Ala Cys Leu Lys Gln Met Tyr Asn Val Gly
210 215 220
Asn Tyr Thr Pro Ser Val Ala Ser Gly Ser Arg Val Gly Phe Gly Ser
225 230 235 240
Phe Leu Asn Gln Ser Ala Ile Tyr Asp Asp Leu Phe Thr Tyr Glu Lys
245 250 255
Val Asn Gly Ile Pro Asn Gln Asn Trp Thr Lys Val Ile Ile Ala Asn
260 265 270
Ala Pro Asn Asp Gln Asp Pro Asn Asp Gly Asn Tyr Gly Glu Ala Asn
275 280 285
Leu Asp Val Gln Asn Ile Val Gly Ile Ser His Pro Leu Pro Val Thr
290 295 300
Glu Phe Leu Thr Gly Gly Ser Pro Pro Phe Val Pro Ser Leu Asp Thr
305 310 315 320
Pro Thr Asn Glu Asn Glu Pro Tyr Ile Pro Tyr Tyr Glu Tyr Leu Leu
325 330 335
Ser Gln Lys Asn Ala Asp Leu Pro Gln Val Ile Ser Asn Ser Tyr Gly
340 345 350
Asp Asp Glu Gln Ser Val Pro Tyr Lys Tyr Ala Val Arg Ala Cys Asn
355 360 365
Leu Ile Gly Leu Thr Gly Leu Arg Gly Ile Thr Val Leu Glu Ser Ser
370 375 380
Gly Asp Leu Gly Val Gly Ala Gly Cys Leu Ser Asn Asp Gly Lys Asn
385 390 395 400
Lys Thr Gln Phe Asp Ala Ile Phe Pro Ala Thr Cys Pro Tyr Ile Thr
405 410 415
Ala Val Gly Gly Thr Gln Ala Val Thr Pro Glu Ile Ala Trp Thr Ala
420 425 430
Ser Ser Gly Gly Phe Ser Tyr Tyr Phe Pro Arg Pro Trp Tyr Gln Arg
435 440 445
Lys Ala Ile Glu Thr Tyr Leu Gly Leu Leu Asp Asp Ser Thr Lys Gln
450 455 460
Tyr Tyr Ser Gln Tyr Thr Asn Phe Glu Gly Arg Gly Phe Pro Asp Val
465 470 475 480
Ala Ala His Ser Leu Leu Pro Asp Tyr Gln Val Val Gly Gly Gly Glu
485 490 495
Leu Gln Arg Ser Gly Gly Thr Ser Ala Ala Ser Pro Val Phe Ala Gly
500 505 510
Ile Ile Ala Leu Leu Asn Asp Ala Arg Leu Ser Ala Gly Lys Pro Thr
515 520 525
Leu Gly Phe Leu Asn Pro Phe Ile Tyr Tyr Ala Gly Tyr Lys Gly Leu
530 535 540
Asn Asp Ile Thr Gly Gly Lys Ser Ile Gly Cys Asn Gly Val Asn Gly
545 550 555 560
Gln Thr Gly Gly Thr Val Pro Gly Gly Gly Val Val Pro Gly Ala Ala
565 570 575
Trp Asn Cys Thr Thr Gly Trp Asp Pro Ala Thr Gly Leu Gly Thr Pro
580 585 590
Asp Phe Gln Lys Leu Lys Glu Leu Val Leu Ser Phe
595 600
<210> 161
<211> 600
<212> PRT
<213> 玉米赤霉
<400> 161
Met Tyr Ile Thr Ser Ser Arg Leu Val Leu Ala Leu Ala Ala Leu Pro
1 5 10 15
Thr Ala Phe Gly Lys Ser Tyr Ser His His Ala Glu Ala Pro Lys Gly
20 25 30
Trp Lys Val Asp Asp Thr Ala Arg Val Ala Ser Thr Gly Lys Gln Gln
35 40 45
Val Phe Ser Ile Ala Leu Thr Met Gln Asn Val Asp Gln Leu Glu Ser
50 55 60
Lys Leu Leu Asp Leu Ser Ser Pro Asp Ser Lys Asn Tyr Gly Gln Trp
65 70 75 80
Met Ser Gln Lys Asp Val Thr Thr Ala Phe Tyr Pro Ser Lys Glu Ala
85 90 95
Val Ser Ser Val Thr Lys Trp Leu Lys Ser Lys Gly Val Lys His Tyr
100 105 110
Asn Val Asn Gly Gly Phe Ile Asp Phe Ala Leu Asp Val Lys Gly Ala
115 120 125
Asn Ala Leu Leu Asp Ser Asp Tyr Gln Tyr Tyr Thr Lys Glu Gly Gln
130 135 140
Thr Lys Leu Arg Thr Leu Ser Tyr Ser Ile Pro Asp Asp Val Ala Glu
145 150 155 160
His Val Gln Phe Val Asp Pro Ser Thr Asn Phe Gly Gly Thr Leu Ala
165 170 175
Phe Ala Pro Val Thr His Pro Ser Arg Thr Leu Thr Glu Arg Lys Asn
180 185 190
Lys Pro Thr Lys Ser Thr Val Asp Ala Ser Cys Gln Thr Ser Ile Thr
195 200 205
Pro Ser Cys Leu Lys Gln Met Tyr Asn Ile Gly Asp Tyr Thr Pro Lys
210 215 220
Val Glu Ser Gly Ser Thr Ile Gly Phe Ser Ser Phe Leu Gly Glu Ser
225 230 235 240
Ala Ile Tyr Ser Asp Val Phe Leu Phe Glu Glu Lys Phe Gly Ile Pro
245 250 255
Thr Gln Asn Phe Thr Thr Val Leu Ile Asn Asn Gly Thr Asp Asp Gln
260 265 270
Asn Thr Ala His Lys Asn Phe Gly Glu Ala Asp Leu Asp Ala Glu Asn
275 280 285
Ile Val Gly Ile Ala His Pro Leu Pro Phe Thr Gln Tyr Ile Thr Gly
290 295 300
Gly Ser Pro Pro Phe Leu Pro Asn Ile Asp Gln Pro Thr Ala Ala Asp
305 310 315 320
Asn Gln Asn Glu Pro Tyr Val Pro Phe Phe Arg Tyr Leu Leu Ser Gln
325 330 335
Lys Glu Val Pro Ala Val Val Ser Thr Ser Tyr Gly Asp Glu Glu Asp
340 345 350
Ser Val Pro Arg Glu Tyr Ala Thr Met Thr Cys Asn Leu Ile Gly Leu
355 360 365
Leu Gly Leu Arg Gly Ile Ser Val Ile Phe Ser Ser Gly Asp Ile Gly
370 375 380
Val Gly Ala Gly Cys Leu Gly Pro Asp His Lys Thr Val Glu Phe Asn
385 390 395 400
Ala Ile Phe Pro Ala Thr Cys Pro Tyr Leu Thr Ser Val Gly Gly Thr
405 410 415
Val Asp Val Thr Pro Glu Ile Ala Trp Glu Gly Ser Ser Gly Gly Phe
420 425 430
Ser Lys Tyr Phe Pro Arg Pro Ser Tyr Gln Asp Lys Ala Val Lys Thr
435 440 445
Tyr Met Lys Thr Val Ser Lys Gln Thr Lys Lys Tyr Tyr Gly Pro Tyr
450 455 460
Thr Asn Trp Glu Gly Arg Gly Phe Pro Asp Val Ala Gly His Ser Val
465 470 475 480
Ser Pro Asn Tyr Glu Val Ile Tyr Ala Gly Lys Gln Ser Ala Ser Gly
485 490 495
Gly Thr Ser Ala Ala Ala Pro Val Trp Ala Ala Ile Val Gly Leu Leu
500 505 510
Asn Asp Ala Arg Phe Arg Ala Gly Lys Pro Ser Leu Gly Trp Leu Asn
515 520 525
Pro Leu Val Tyr Lys Tyr Gly Pro Lys Val Leu Thr Asp Ile Thr Gly
530 535 540
Gly Tyr Ala Ile Gly Cys Asp Gly Asn Asn Thr Gln Ser Gly Lys Pro
545 550 555 560
Glu Pro Ala Gly Ser Gly Ile Val Pro Gly Ala Arg Trp Asn Ala Thr
565 570 575
Ala Gly Trp Asp Pro Val Thr Gly Tyr Gly Thr Pro Asp Phe Gly Lys
580 585 590
Leu Lys Asp Leu Val Leu Ser Phe
595 600
<210> 162
<211> 599
<212> PRT
<213> 尖孢镰刀菌
<400> 162
Met Tyr Ile Ser Ser Gln Asn Leu Val Leu Ala Leu Ser Ala Leu Pro
1 5 10 15
Ser Ala Phe Gly Lys Ser Phe Ser His His Ala Glu Ala Pro Gln Gly
20 25 30
Trp Gln Val Gln Lys Ala Ala Lys Val Ala Ser Asn Thr Gln His Val
35 40 45
Phe Ser Leu Ala Leu Thr Met Gln Asn Val Asp Gln Leu Glu Ser Lys
50 55 60
Leu Leu Asp Leu Ser Ser Pro Asp Ser Ala Asn Tyr Gly Asn Trp Leu
65 70 75 80
Ser His Asp Glu Leu Thr Ser Thr Phe Ser Pro Ser Lys Glu Ala Val
85 90 95
Ala Ser Val Thr Lys Trp Leu Lys Ser Lys Gly Ile Lys His Tyr Lys
100 105 110
Val Asn Gly Ala Phe Ile Asp Phe Ala Ala Asp Val Glu Lys Ala Asn
115 120 125
Thr Leu Leu Gly Gly Asp Tyr Gln Tyr Tyr Thr Lys Asp Gly Gln Thr
130 135 140
Lys Leu Arg Thr Leu Ser Tyr Ser Ile Pro Asp Asp Val Ala Gly His
145 150 155 160
Val Gln Phe Val Asp Pro Ser Thr Asn Phe Gly Gly Thr Val Ala Phe
165 170 175
Asn Pro Val Pro His Pro Ser Arg Thr Leu Gln Glu Arg Lys Val Ser
180 185 190
Pro Ser Lys Ser Thr Val Asp Ala Ser Cys Gln Thr Ser Ile Thr Pro
195 200 205
Ser Cys Leu Lys Gln Met Tyr Asn Val Gly Asp Tyr Thr Pro Asp Ala
210 215 220
Lys Ser Gly Ser Glu Ile Gly Phe Ser Ser Phe Leu Gly Gln Ala Ala
225 230 235 240
Ile Tyr Ser Asp Val Phe Lys Phe Glu Glu Leu Phe Gly Ile Pro Lys
245 250 255
Gln Asn Tyr Thr Thr Ile Leu Ile Asn Asn Gly Thr Asp Asp Gln Asn
260 265 270
Thr Ala His Lys Asn Tyr Gly Glu Ala Asn Leu Asp Ala Glu Asn Ile
275 280 285
Val Gly Ile Ala His Pro Leu Pro Phe Lys Gln Tyr Ile Thr Gly Gly
290 295 300
Ser Pro Pro Phe Val Pro Asn Ile Asp Gln Pro Thr Glu Lys Asp Asn
305 310 315 320
Gln Asn Glu Pro Tyr Val Pro Phe Phe Arg Tyr Leu Leu Gly Gln Lys
325 330 335
Asp Leu Pro Ala Val Ile Ser Thr Ser Tyr Gly Asp Glu Glu Asp Ser
340 345 350
Val Pro Arg Glu Tyr Ala Thr Leu Thr Cys Asn Met Ile Gly Leu Leu
355 360 365
Gly Leu Arg Gly Ile Ser Val Ile Phe Ser Ser Gly Asp Ile Gly Val
370 375 380
Gly Ser Gly Cys Leu Ala Pro Asp Tyr Lys Thr Val Glu Phe Asn Ala
385 390 395 400
Ile Phe Pro Ala Thr Cys Pro Tyr Leu Thr Ser Val Gly Gly Thr Val
405 410 415
Asp Val Thr Pro Glu Ile Ala Trp Glu Gly Ser Ser Gly Gly Phe Ser
420 425 430
Lys Tyr Phe Pro Arg Pro Ser Tyr Gln Asp Lys Ala Ile Lys Lys Tyr
435 440 445
Met Lys Thr Val Ser Lys Glu Thr Lys Lys Tyr Tyr Gly Pro Tyr Thr
450 455 460
Asn Trp Glu Gly Arg Gly Phe Pro Asp Val Ala Gly His Ser Val Ala
465 470 475 480
Pro Asp Tyr Glu Val Ile Tyr Asn Gly Lys Gln Ala Arg Ser Gly Gly
485 490 495
Thr Ser Ala Ala Ala Pro Val Trp Ala Ala Ile Val Gly Leu Leu Asn
500 505 510
Asp Ala Arg Phe Lys Ala Gly Lys Lys Ser Leu Gly Trp Leu Asn Pro
515 520 525
Leu Ile Tyr Lys His Gly Pro Lys Val Leu Thr Asp Ile Thr Gly Gly
530 535 540
Tyr Ala Ile Gly Cys Asp Gly Asn Asn Thr Gln Ser Gly Gln Pro Glu
545 550 555 560
Pro Ala Gly Ser Gly Leu Val Pro Gly Ala Arg Trp Asn Ala Thr Ala
565 570 575
Gly Trp Asp Pro Thr Thr Gly Tyr Gly Thr Pro Asn Phe Gln Lys Leu
580 585 590
Lys Asp Leu Val Leu Ser Phe
595
<210> 163
<211> 637
<212> PRT
<213> Grosmannia clavigera
<400> 163
Met Ala Thr Glu His His Ala Arg Thr Arg Ala Ala Val Val Leu His
1 5 10 15
Thr Leu Arg Gly Ser Met Ala Thr Leu Ala Leu Ala Val Ser Ser Leu
20 25 30
Leu Leu Leu Pro Ala Ala Glu Ala Ala Val Ile Ser Ser Ile Asp Thr
35 40 45
Val Pro Lys Gly Trp Ser Val Ala Pro Ala Ala Lys Ile Asn Ala Arg
50 55 60
Thr Ser Ser Ala Gln Val Phe Thr Ile Ala Leu Ala Leu Gln Asn Ile
65 70 75 80
Asp Gln Leu Glu Ser His Leu Leu Ser Val Ser Ser Pro Asn Ser Ala
85 90 95
Ser Tyr Gly Ala His Glu Asp Val Glu Gly Ile Thr Ala Lys Tyr Gly
100 105 110
Pro Ser Asp Glu Ala Val Gln Val Val Thr Ala Trp Leu Lys Ser Ser
115 120 125
Ser Ala Val Gln Thr Phe Ser Val Arg Asp Gly Phe Val Asp Val Ala
130 135 140
Ala Thr Val Asp Gly Ala Asn Thr Leu Phe Asn Ala Ser Tyr Asp Phe
145 150 155 160
Tyr Thr His Glu Ala Asn Ala Ala Thr Thr Lys Leu Arg Thr Leu Ser
165 170 175
Tyr Ser Val Pro Asp His Val Ala Ala His Val Ala Leu Val Asp Pro
180 185 190
Gly Asn Tyr Phe Gly Lys Thr Ala Ala Phe Ala Pro Arg Arg Gly Ala
195 200 205
Pro Ala Pro Val Pro Ala Ser Lys Thr Ala Arg Ser Pro Ser Leu Val
210 215 220
Lys Ala Arg Gly Thr Ser Thr Thr Ile Asp Ala Ala Cys Ser Thr Ser
225 230 235 240
Ile Thr Pro Ala Cys Leu Lys Gln Leu Tyr Asn Val Gly Asn Tyr Thr
245 250 255
Pro Asp Ala Ser Ser Gly Ser Arg Val Gly Phe Gly Ser Phe Leu Asn
260 265 270
Gln Ser Ala Leu Tyr Ala Asp Val Phe Thr Tyr Glu Glu Arg Asn Gly
275 280 285
Ile Ala Ala Gln Asn Phe Thr Val Glu Leu Ile Ala Asn Ala Thr Asn
290 295 300
Asp Gln Asp Lys Trp Thr Ala Ser Val Asp Glu Ala Asn Leu Asp Val
305 310 315 320
Gln Asn Ile Ile Gly Val Ser His Pro Leu Pro Val Ile Glu Tyr Ile
325 330 335
Thr Gly Gly Ser Pro Pro Phe Val Ala Asp Ile Asp Leu Pro Ser Ala
340 345 350
Ala Glu Asn Gln Asn Glu Pro Tyr Leu Pro Tyr Leu Ser Tyr Leu Leu
355 360 365
Ala Lys Ser Asn Ser Glu Leu Pro Gln Ala Ile Ser Asn Ser Tyr Gly
370 375 380
Asp Glu Glu Asp Ser Val Pro Tyr Asn Tyr Ala Asn Tyr Thr Cys Asn
385 390 395 400
Leu Ile Gly Met Leu Gly Leu Arg Gly Ile Thr Val Leu His Ser Ser
405 410 415
Gly Asp Met Gly Val Gly Ala Gly Cys Leu Ala Pro Asp Asn Thr Thr
420 425 430
Val Glu Phe Asn Pro Ile Tyr Pro Ala Thr Cys Pro Tyr Val Thr Ala
435 440 445
Val Gly Gly Thr Val Asn Val Ser Pro Glu Ile Ala Trp Lys Gly Ser
450 455 460
Ser Gly Gly Phe Ser Lys Tyr Phe Pro Arg Pro Ser Tyr Gln Asp Lys
465 470 475 480
Ala Val Ser Glu Tyr Leu Ser Asp His Val Asn Ala Ser Thr Val Lys
485 490 495
Tyr Tyr Ala Pro Tyr Thr Asn Phe Ser Gly Arg Gly Phe Pro Asp Val
500 505 510
Ser Ala His Ser Val Ser Pro Ala Tyr Glu Ile Ile Tyr Phe Asp Gln
515 520 525
Pro Gly Pro Ser Gly Gly Thr Ser Ala Ala Ala Pro Val Trp Ala Gly
530 535 540
Ile Val Gly Leu Leu Asn Asp Ala Arg Leu Arg Ser Gly Lys Ser Thr
545 550 555 560
Leu Gly Trp Leu Asn Pro Leu Ile Tyr Thr Tyr Gly Pro Ser Val Leu
565 570 575
Asn Asp Ile Thr Asp Gly Tyr Ser Val Gly Cys Asn Gly Val Asn Tyr
580 585 590
Gln Thr Asp Gln Tyr Glu Pro Ala Gly Ala Gly Ile Val Pro Gly Ala
595 600 605
His Trp Asn Ala Thr Val Gly Trp Asp Pro Val Thr Gly Tyr Gly Thr
610 615 620
Pro Asn Phe Glu Glu Leu Lys Lys Val Val Leu Gly Leu
625 630 635
<210> 164
<211> 594
<212> PRT
<213> 黑白轮枝菌
<400> 164
Met Val Leu Pro Lys Leu His Ala Cys Val Ser Arg Ala Ala Thr Leu
1 5 10 15
Pro Ala Ser Ile Gln Leu Ile Arg Gln Ala Pro Ala Gly Asp Glu Ile
20 25 30
Gln Leu Gln Ile Ala Leu Lys His Gln Asn Ile His Lys Leu Glu Ser
35 40 45
Thr Leu Arg Ser Val Ser Asp Pro Ser Ser Ser His Tyr Gly Gln Tyr
50 55 60
Leu Ser Lys Asp Glu Val Asn Ala Leu Phe Ala Pro Ser Asn His Ser
65 70 75 80
Arg Thr Ala Val Glu Ser Trp Leu Lys Asp Ala Gly Ile Thr Ala Val
85 90 95
Ser Thr Arg Gly Pro Leu Val Asp Phe Val Thr Thr Val Ala Lys Ala
100 105 110
Asn Glu Leu Leu Gly Ala Asp Phe Ala Trp Phe Asp Val Glu Gly His
115 120 125
Lys Lys Leu Arg Thr Thr Glu Tyr Ser Ile Pro Asp Thr Leu Thr Ala
130 135 140
His Ile Asp Leu Val Ala Pro Thr Thr Phe Phe Gly Arg Thr Lys Ser
145 150 155 160
His Arg Met Ile Pro Asp Asp Glu Thr Ser Glu Leu Asp Ala Arg Gln
165 170 175
Ala Arg Asn Thr Thr Thr Cys Leu Ser Val Ile Thr Pro Asp Cys Leu
180 185 190
Glu Glu Ala Tyr Asn Tyr Gly Ser Tyr Lys Ala Asp Pro Ala Ala Gly
195 200 205
Ser Arg Val Ala Phe Ala Ser Phe Leu Asn Gln Ser Ala Arg Gln Glu
210 215 220
Asp Leu Thr Gln Tyr Leu Asn Thr Phe Lys Leu Pro Pro Arg Asn Phe
225 230 235 240
Thr Thr Val Leu Ile Asn Gly Gly Glu Asp His Gln Asp Pro Asn Gly
245 250 255
Asp Ile Gly Glu Ala Asn Leu Asp Ala Gln Phe Leu Ala Ala Val Thr
260 265 270
Lys Asn Leu Pro Leu Lys Gln Phe Ile Thr Gly Gly Ser Pro Pro Phe
275 280 285
Val Pro Asn Leu Arg Ile Thr Asn Ala Ser Thr Asn Ser Asn Glu Pro
290 295 300
Tyr Leu Asp Phe Tyr Glu Thr Leu Leu Ala Thr Asp Asp Glu Gly Val
305 310 315 320
Pro Gln Val Leu Ser Ser Ser Tyr Gly Asp Asp Glu Gln Thr Val Pro
325 330 335
Val Glu Tyr Ala Lys Arg Val Cys Asn Leu Ile Gly Met Met Gly Leu
340 345 350
Arg Gly Val Thr Val Leu Glu Ser Ser Gly Asp Ala Gly Val Gly Ala
355 360 365
Pro Cys Arg Ala Asn Asp Gly Ser Gly Arg Ala Glu Phe Thr Pro Thr
370 375 380
Phe Pro Gly Thr Cys Pro Tyr Leu Thr Ala Val Gly Gly Thr Gln Ala
385 390 395 400
Trp Ala Pro Glu Val Ala Trp Val Gly Ser Ala Gly Gly Phe Ser Asn
405 410 415
Tyr Phe Glu Arg Ala Trp Tyr Gln Lys Ala Ala Val Lys Thr Tyr Leu
420 425 430
Lys Glu Ser Ile Pro Ala Glu Val Lys Ser Tyr Tyr Lys His Gly Gly
435 440 445
Phe Val Asp Phe Gly Gly Arg Gly Phe Pro Asp Ile Ser Ala His Ser
450 455 460
Leu Glu Pro Trp Tyr Gln Phe Val Asn Ala Gly Arg Pro Arg Arg Thr
465 470 475 480
Gly Gly Thr Ser Ala Ala Ala Pro Val Val Ala Gly Ile Ile Gly Leu
485 490 495
Leu Asn Asp Ala Arg Leu Arg Ala Gly Gln Pro Thr Met Gly Phe Leu
500 505 510
Asn Pro Trp Leu Tyr Ala Arg Gly Val Asp Gly Leu Leu Asp Val Thr
515 520 525
Glu Gly Lys Ala Glu Gly Cys Gly Gly Ile Asp Leu Gln Ser Gly Asn
530 535 540
Pro Val Pro Gly Ala Gly Val Ile Pro Trp Ala Ser Trp Asn Ala Thr
545 550 555 560
Lys Gly Ala Ala Thr Asp Cys Gly Gln Trp His Gln Gly Met Ile Trp
565 570 575
Cys Cys Trp Val Pro Val Val Ala Met Tyr Leu Glu Met Leu Arg Glu
580 585 590
Gln Gly
<210> 165
<211> 566
<212> PRT
<213> 大丽轮枝菌
<400> 165
Met Val Leu Leu Lys Leu His Ala Cys Val Ala Ala Thr Leu Val Ala
1 5 10 15
Leu Thr Glu Ala Leu Leu Val Ser Arg Ala Ala Thr Leu Pro Ala Ser
20 25 30
Ile Gln Leu Ile Arg Gln Ala Pro Ala Gly Asp Glu Ile Gln Leu Gln
35 40 45
Ile Ala Leu Lys His Gln Asn Ile His Lys Leu Glu Ser Lys Leu Arg
50 55 60
Ser Val Ser Asp Pro Ser Ser Ser His Tyr Gly Gln Tyr Leu Ser Lys
65 70 75 80
Asp Glu Val Asn Ala Leu Phe Ala Pro Ser Asn His Ser Arg Thr Ala
85 90 95
Val Glu Ser Trp Leu Lys Asp Ala Gly Ile Thr Ala Val Ser Ser Arg
100 105 110
Gly Pro Leu Val Asp Phe Val Thr Thr Val Ala Lys Ala Asn Glu Leu
115 120 125
Leu Gly Ala Asp Phe Ala Trp Phe Asp Val Glu Gly His Lys Lys Leu
130 135 140
Arg Thr Thr Glu Tyr Ser Ile Pro Asp Thr Leu Thr Ala His Ile Asp
145 150 155 160
Leu Val Ala Pro Thr Thr Phe Phe Gly Arg Thr Lys Ser His Arg Met
165 170 175
Ile Pro Asp Asp Glu Thr Ser Glu Leu Asp Ser Arg Gln Ala Arg Asn
180 185 190
Thr Thr Thr Cys Leu Ser Val Ile Thr Pro Asp Cys Leu Glu Glu Ala
195 200 205
Tyr Asn Tyr Gly Ser Tyr Lys Ala Asp Pro Ala Ala Gly Ser Arg Val
210 215 220
Ala Phe Ala Ser Phe Leu Asn Gln Ser Ala Arg Gln Glu Asp Leu Thr
225 230 235 240
Gln Tyr Leu Asn Thr Phe Lys Leu Pro Pro Arg Asn Phe Thr Thr Val
245 250 255
Leu Ile Asn Gly Gly Glu Asp His Gln Asp Pro Asn Gly Asp Ile Gly
260 265 270
Glu Ala Asn Leu Asp Ala Gln Phe Leu Ala Ala Val Thr Lys Asn Leu
275 280 285
Pro Leu Lys Gln Phe Ile Thr Gly Gly Ser Pro Pro Phe Val Pro Asn
290 295 300
Leu Arg Ile Thr Asn Ala Ser Thr Asn Ser Asn Glu Pro Tyr Leu Asp
305 310 315 320
Phe Tyr Glu Thr Leu Leu Ala Thr Asp Asp Glu Gly Val Pro Gln Val
325 330 335
Leu Ser Ser Ser Tyr Gly Asp Asp Glu Gln Thr Val Pro Val Glu Tyr
340 345 350
Ala Lys Arg Val Cys Asn Leu Ile Gly Met Met Gly Leu Arg Gly Val
355 360 365
Thr Val Leu Glu Ser Ser Gly Asp Ala Gly Ala Trp Ala Pro Glu Val
370 375 380
Ala Trp Val Gly Ser Ala Gly Gly Phe Ser Asn Tyr Phe Glu Arg Ala
385 390 395 400
Trp Tyr Gln Lys Ala Ala Val Lys Thr Tyr Leu Glu Glu Ser Ile Pro
405 410 415
Val Glu Val Lys Ser Tyr Tyr Lys Arg Gly Gly Phe Val Asp Phe Gly
420 425 430
Gly Arg Gly Phe Pro Asp Ile Ser Ala His Ser Leu Glu Pro Trp Tyr
435 440 445
Gln Phe Val Asn Ala Gly Arg Pro Arg Arg Thr Gly Gly Thr Ser Ala
450 455 460
Ala Ala Pro Val Val Ala Gly Ile Ile Gly Leu Leu Asn Asp Ala Arg
465 470 475 480
Leu Arg Ala Gly Gln Pro Thr Met Gly Phe Leu Asn Pro Trp Leu Tyr
485 490 495
Ala Arg Gly Val Asp Gly Leu Leu Asp Val Thr Glu Gly Lys Ala Glu
500 505 510
Gly Cys Gly Gly Ile Asp Leu Gln Ser Gly Asn Pro Val Pro Gly Ala
515 520 525
Gly Val Ile Pro Trp Ala Ser Trp Asn Ala Thr Lys Gly Trp Asp Pro
530 535 540
Val Thr Gly Leu Gly Leu Pro Asp Phe Glu Ala Leu Arg Gln Ile Ala
545 550 555 560
Val Asn Gly Thr Lys Ala
565
<210> 166
<211> 391
<212> PRT
<213> 里氏木霉
<400> 166
Met Arg Leu Ser Val Leu Leu Ser Val Leu Pro Leu Val Leu Ala Ala
1 5 10 15
Pro Ala Ile Glu Lys Arg Ala Glu Pro Ala Pro Leu Leu Val Pro Thr
20 25 30
Thr Lys His Gly Leu Val Ala Asp Lys Tyr Ile Val Lys Phe Lys Asp
35 40 45
Gly Ser Ser Leu Gln Ala Val Asp Glu Ala Ile Ser Gly Leu Val Ser
50 55 60
Asn Ala Asp His Val Tyr Gln His Val Phe Arg Gly Phe Ala Ala Thr
65 70 75 80
Leu Asp Lys Glu Thr Leu Glu Ala Leu Arg Asn His Pro Glu Val Asp
85 90 95
Tyr Ile Glu Gln Asp Ala Val Val Lys Ile Asn Ala Tyr Val Ser Gln
100 105 110
Thr Gly Ala Pro Trp Gly Leu Gly Arg Ile Ser His Lys Ala Arg Gly
115 120 125
Ser Thr Thr Tyr Val Tyr Asp Asp Ser Ala Gly Ala Gly Thr Cys Ser
130 135 140
Tyr Val Ile Asp Thr Gly Val Asp Ala Thr His Pro Asp Phe Glu Gly
145 150 155 160
Arg Ala Thr Leu Leu Arg Ser Phe Val Ser Gly Gln Asn Thr Asp Gly
165 170 175
Asn Gly His Gly Thr His Val Ser Gly Thr Ile Gly Ser Arg Thr Tyr
180 185 190
Gly Val Ala Lys Lys Thr Gln Ile Tyr Gly Val Lys Val Leu Asp Asn
195 200 205
Ser Gly Ser Gly Ser Phe Ser Thr Val Ile Ala Gly Met Asp Tyr Val
210 215 220
Ala Ser Asp Ser Gln Thr Arg Asn Cys Pro Asn Gly Ser Val Ala Asn
225 230 235 240
Met Ser Leu Gly Gly Gly Tyr Thr Ala Ser Val Asn Gln Ala Ala Ala
245 250 255
Arg Leu Ile Gln Ala Gly Val Phe Leu Ala Val Ala Ala Gly Asn Asp
260 265 270
Gly Val Asp Ala Arg Asn Thr Ser Pro Ala Ser Glu Pro Thr Val Cys
275 280 285
Thr Val Gly Ala Ser Thr Ser Ser Asp Ala Arg Ala Ser Phe Ser Asn
290 295 300
Tyr Gly Ser Val Val Asp Ile Phe Ala Pro Gly Gln Asp Ile Leu Ser
305 310 315 320
Thr Trp Pro Asn Arg Gln Thr Asn Thr Ile Ser Gly Thr Ser Met Ala
325 330 335
Thr Pro His Ile Val Gly Leu Gly Ala Tyr Leu Ala Gly Leu Glu Gly
340 345 350
Phe Ser Asp Pro Gln Ala Leu Cys Ala Arg Ile Gln Ser Leu Ala Asn
355 360 365
Arg Asn Leu Leu Ser Gly Ile Pro Ser Gly Thr Ile Asn Ala Ile Ala
370 375 380
Phe Asn Gly Asn Pro Ser Gly
385 390
<210> 167
<211> 371
<212> PRT
<213> 深绿木霉
<400> 167
Met Ala Ala Pro Ser Arg Arg Ala Ser Pro Ala Pro Val Leu Val Pro
1 5 10 15
Arg Gly Ala Gln Val Leu Glu Gly Arg Tyr Ile Val Lys Met Lys Thr
20 25 30
Glu Ala Gln Val Ser Ala Val Glu Ser Ala Leu Ser Gly Val Ser Ala
35 40 45
Gly Ala Ser His Arg Tyr Ser His Ser Phe Ser Gly Phe Ala Ala Ser
50 55 60
Leu Thr Pro Gly Glu Leu Glu Ile Leu Gln Asn Asp Pro Asn Val Asp
65 70 75 80
Phe Ile Glu Gln Asp Ala Thr Val Ser Thr Lys Ala Ile Val Thr Glu
85 90 95
Gly Ser Ala Asp Trp Gly Leu Ser Arg Ile Ser Asn Lys Lys Pro Asp
100 105 110
Thr Thr Arg Tyr Ser Tyr Asp Ser Ser Ala Gly Glu Gly Thr Cys Ala
115 120 125
Phe Val Ile Asp Thr Gly Ile Asp Val His His Pro Glu Phe Glu Gly
130 135 140
Arg Ala Lys Phe Leu Glu Asn Phe Val Gly Asp Gly Ser Asp Ala Asp
145 150 155 160
Leu Phe Gly His Gly Thr His Val Ser Gly Ile Ile Ala Gly Lys Thr
165 170 175
Phe Gly Val Ala Lys Lys Ala Ser Leu Phe Ala Val Lys Val Leu Asp
180 185 190
Val Asn Gly Gln Gly Asn Asn Ser Val Ile Ile Ala Gly Met Asp Tyr
195 200 205
Val Ala Lys Asn Ala Ser Ser Gln His Cys Pro Lys Gly Ile Val Val
210 215 220
Asn Met Ser Leu Gly Gly Ile Lys Thr Asp Ala Val Asn Gln Ala Ala
225 230 235 240
Ile Ser Ile Thr Asn Ala Gly Leu Phe Leu Ala Ala Ala Ala Gly Asn
245 250 255
Asp Ala Leu Asp Ala Ala Asp Phe Ser Pro Ala Cys Val Pro Ala Val
260 265 270
Cys Thr Val Gly Ala Thr Asp Arg Asn Asp Thr Phe Ala Leu Tyr Ser
275 280 285
Asn Arg Gly Ser Ala Val Asp Ile Leu Ala Pro Gly Thr Asn Ile Thr
290 295 300
Ser Ser Trp Pro Gly Asn Leu Thr Lys Val Met Ser Gly Thr Ser Met
305 310 315 320
Ala Ser Pro His Val Ala Gly Leu Gly Ala Tyr Leu Leu Gly Leu Gly
325 330 335
Gln Lys Ile Gly Gly Leu Cys Gly Thr Ile Ser Ser Ser Ala Leu Lys
340 345 350
Gly Ala Ile Lys Gly Val Pro Glu Ser Thr Val Asn Leu Leu Val Gln
355 360 365
Asn Gly Ala
370
<210> 168
<211> 391
<212> PRT
<213> 绿木霉
<400> 168
Met Arg Leu Ser Val Leu Leu Ser Leu Leu Pro Met Val Leu Ala Ala
1 5 10 15
Pro Ala Ile Glu Lys Arg Ala Glu Pro Ala Pro Leu Phe Lys Pro Thr
20 25 30
Ser Lys His Gly Leu Val Ala Asp Gln Tyr Ile Val Lys Phe Lys Glu
35 40 45
Gly Ser Ser Leu Gln Ala Val Asp Glu Thr Leu Thr Arg Ile Ala Ser
50 55 60
Asp Ala His His Val Tyr Gln His Val Phe Asn Gly Phe Ala Gly Lys
65 70 75 80
Leu Asp Gln Glu Thr Leu Glu Ala Leu Arg Asn His Pro Asp Val Glu
85 90 95
Tyr Ile Glu Gln Asp Ala Ile Val Lys Val Asn Ala Phe Val Gln Gln
100 105 110
Thr Gly Ala Pro Trp Gly Leu Gly Arg Ile Ser His Lys Ala Arg Gly
115 120 125
Ser Thr Thr Tyr Val Tyr Asp Asp Ser Ala Gly Ala Gly Thr Cys Ala
130 135 140
Tyr Ile Ile Asp Thr Gly Val Asp Ala Thr His Pro Glu Phe Glu Gly
145 150 155 160
Arg Ala Thr Phe Leu Lys Thr Tyr Val Ser Gly Gln Asn Thr Asp Gly
165 170 175
Asn Gly His Gly Thr His Val Ala Gly Thr Ile Gly Ser Arg Ser Tyr
180 185 190
Gly Val Ala Lys Lys Thr Gln Leu Phe Gly Val Lys Val Leu Asp Asn
195 200 205
Ser Gly Ser Gly Thr Phe Ala Asn Val Ile Ala Gly Met Asp Phe Val
210 215 220
Ala Ser Asp Ser Lys Thr Arg Asn Cys Pro Asn Gly Ser Val Ala Asn
225 230 235 240
Met Ser Leu Gly Gly Gly Val Ser Ser Ala Val Asn Glu Ala Ala Ala
245 250 255
Ala Leu Ile Ala Ala Asp Val Phe Leu Ala Val Ala Ala Gly Asn Asp
260 265 270
Gly Thr Asp Ala Arg Asn Thr Ser Pro Ala Ser Glu Pro Thr Val Cys
275 280 285
Thr Val Gly Ala Thr Thr Ser Ala Asp Ala Arg Ala Ser Phe Ser Asn
290 295 300
Tyr Gly Ser Val Val Asp Ile Phe Ala Pro Gly Gln Asp Ile Leu Ser
305 310 315 320
Thr Trp Pro Ser Gly Arg Thr Asn Thr Ile Ser Gly Thr Ser Met Ala
325 330 335
Thr Pro His Ile Val Gly Leu Gly Ala Tyr Leu Leu Ser Leu Glu Gly
340 345 350
His Ile Ala Pro Gln Ala Leu Cys Ser Arg Ile Gln Ser Leu Ala Gly
355 360 365
Lys Ser Leu Leu Ser Gly Ile Pro Ser Gly Thr Ile Asn Ala Val Ala
370 375 380
Phe Asn Gly Asn Pro Ser Gly
385 390
<210> 169
<211> 391
<212> PRT
<213> 康宁肉座菌
<400> 169
Met Arg Leu Ser Val Leu Leu Ser Val Leu Pro Leu Val Leu Val Ala
1 5 10 15
Pro Ala Thr Glu Lys Arg Ala Glu Pro Ala Pro Leu Leu Val Pro Thr
20 25 30
Thr Lys His Gly Leu Val Ala Asp Lys Tyr Ile Val Lys Phe Lys Asp
35 40 45
Gly Ser Ser Leu Gln Ala Val Asp Glu Ala Ile Ser Gly Leu Val Ser
50 55 60
Asn Ala Asp His Val Tyr Gln His Val Phe Arg Gly Phe Ala Ala Thr
65 70 75 80
Leu Asp Lys Lys Thr Leu Glu Thr Leu Arg Asn His Pro Glu Val Asp
85 90 95
Tyr Ile Glu Gln Asp Ala Val Val Lys Ile Asn Ser Tyr Val Ser Gln
100 105 110
Ser Gly Ala Pro Trp Gly Leu Gly Arg Ile Ser His Lys Ala Arg Gly
115 120 125
Ser Thr Thr Tyr Val Tyr Asp Asp Ser Ala Gly Ala Gly Thr Cys Ser
130 135 140
Tyr Val Ile Asp Thr Gly Val Asp Ala Ala His Pro Asp Phe Glu Gly
145 150 155 160
Arg Ala Thr Leu Leu Arg Ser Phe Val Ser Gly Gln Asn Thr Asp Gly
165 170 175
Asn Gly His Gly Thr His Val Ser Gly Thr Ile Gly Ser Arg Thr Tyr
180 185 190
Gly Val Ala Lys Lys Thr Gln Ile Tyr Gly Ile Lys Val Leu Asp Asn
195 200 205
Ser Gly Ser Gly Ser Phe Ser Thr Val Ile Ala Gly Met Asp Tyr Val
210 215 220
Ala Ser Asp Ser Gln Thr Arg Asn Cys Pro Asn Gly Ser Val Ala Asn
225 230 235 240
Met Ser Leu Gly Gly Gly Tyr Thr Ala Ser Val Asn Gln Ala Ala Ala
245 250 255
Arg Leu Ile Gln Ala Gly Val Phe Leu Ala Val Ala Ala Gly Asn Asp
260 265 270
Gly Val Asp Ala Arg Asn Thr Ser Pro Ala Ser Glu Pro Ser Val Cys
275 280 285
Thr Val Gly Ala Ser Thr Ser Ser Asp Ala Arg Ala Ser Phe Ser Asn
290 295 300
Tyr Gly Ser Val Val Asp Ile Phe Ala Pro Gly Gln Asp Ile Leu Ser
305 310 315 320
Thr Trp Pro Asn Arg Gln Thr Asn Ala Ile Ser Gly Thr Ser Met Ala
325 330 335
Thr Pro His Ile Val Gly Leu Gly Ala Tyr Leu Ala Gly Leu Glu Gly
340 345 350
Phe Ser Asp Pro Gln Ala Leu Cys Ala Arg Ile Gln Ala Leu Ala Gly
355 360 365
Arg Asn Leu Leu Ser Gly Ile Pro Ser Gly Thr Ile Asn Ala Ile Ala
370 375 380
Phe Asn Gly Asn Pro Ser Gly
385 390
<210> 170
<211> 390
<212> PRT
<213> 淡紫紫孢菌
<400> 170
Met Lys Leu Ser Leu Leu Phe Ala Ile Leu Pro Ala Val Leu Ala Ala
1 5 10 15
Pro Ala Val Lys Arg Asp Glu Pro Ala Pro Leu Leu Thr Pro Arg Gly
20 25 30
Ala Ser Gln Leu Ile Asn Gly Lys Tyr Ile Val Lys Phe Lys Asp Gly
35 40 45
Met Ser Ile Ala Ser Val Asp Lys Thr Val Ser Ala Leu Ser Ser Lys
50 55 60
Ala Asp Arg Val Tyr Asn His Ile Phe Arg Gly Phe Ala Gly Asn Leu
65 70 75 80
Asn Ala Asn Asp Leu Lys Thr Leu Arg Asp His Pro Asp Val Glu Tyr
85 90 95
Ile Glu Gln Asp Ala Ile Ile Thr Ile Asn Ala Tyr Thr Gln Gln Pro
100 105 110
Gly Ala Pro Trp Gly Leu Gly Arg Ile Ser His Arg Ser Lys Gly Ser
115 120 125
Thr Thr Tyr Glu Tyr Asp Thr Ser Gly Gly Ser Gly Thr Cys Ala Tyr
130 135 140
Val Ile Asp Thr Gly Val Glu Ala Ser His Pro Glu Phe Glu Gly Arg
145 150 155 160
Ala Ser Gln Ile Lys Ser Phe Ile Ser Gly Gln Asn Thr Asp Gly Asn
165 170 175
Gly His Gly Thr His Cys Ala Gly Thr Ile Gly Ser Lys Thr Tyr Gly
180 185 190
Val Ala Lys Lys Thr Lys Ile Tyr Gly Val Lys Val Leu Asp Asn Ser
195 200 205
Gly Ser Gly Ser Tyr Ser Gly Ile Ile Ser Gly Met Asp Phe Ala Val
210 215 220
Gln Asp Ser Lys Thr Arg Ser Cys Pro Lys Gly Val Val Ala Asn Met
225 230 235 240
Ser Leu Gly Gly Gly Lys Ala Gln Ser Val Asn Asp Gly Ala Ala Ala
245 250 255
Met Ile Arg Ala Gly Val Phe Leu Ala Val Ala Ala Gly Asn Asp Asn
260 265 270
Ala Asn Ala Ala Asn Tyr Ser Pro Ala Ser Glu Pro Thr Val Cys Thr
275 280 285
Val Gly Ala Thr Thr Ser Ser Asp Ala Arg Ser Ser Phe Ser Asn Tyr
290 295 300
Gly Ser Leu Val Asp Ile Phe Ala Pro Gly Ser Asn Ile Leu Ser Thr
305 310 315 320
Trp Ile Gly Gly Thr Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Thr
325 330 335
Pro His Ile Val Gly Leu Gly Ala Tyr Leu Ala Gly Leu Glu Gly Phe
340 345 350
Pro Gly Ala Gln Ala Leu Cys Lys Arg Ile Gln Thr Leu Ser Thr Lys
355 360 365
Asn Val Leu Thr Gly Ile Pro Ser Gly Thr Val Asn Tyr Leu Ala Phe
370 375 380
Asn Gly Asn Pro Ser Gly
385 390
<210> 171
<211> 388
<212> PRT
<213> 金龟子绿僵菌
<400> 171
Met His Leu Ser Ala Leu Leu Thr Leu Leu Pro Ala Val Leu Ala Ala
1 5 10 15
Pro Ala Thr Ile Gly Arg Arg Ala Glu Pro Ala Pro Leu Phe Thr Pro
20 25 30
Gln Ala Glu Ser Ile Ile Ala Asp Lys Tyr Ile Val Lys Phe Lys Asp
35 40 45
Asp Ile Ala Arg Ile Ala Thr Asp Asp Thr Val Ser Ala Leu Thr Ser
50 55 60
Lys Ala Asp Phe Val Tyr Glu His Ala Phe His Gly Phe Ala Gly Ser
65 70 75 80
Leu Thr Lys Glu Glu Leu Lys Met Leu Arg Glu His Pro Gly Val Asp
85 90 95
Phe Ile Glu Lys Asp Ala Val Met Arg Ile Ser Gly Ile Thr Glu Gln
100 105 110
Ser Gly Ala Pro Trp Gly Leu Gly Arg Ile Ser His Arg Ser Lys Gly
115 120 125
Ser Thr Thr Tyr Arg Tyr Asp Asp Ser Ala Gly Gln Gly Thr Cys Val
130 135 140
Tyr Ile Ile Asp Thr Gly Ile Glu Ala Ser His Pro Glu Phe Glu Gly
145 150 155 160
Arg Ala Thr Phe Leu Lys Ser Phe Ile Ser Gly Gln Asn Thr Asp Gly
165 170 175
His Gly His Gly Thr His Cys Ala Gly Thr Ile Gly Ser Lys Thr Tyr
180 185 190
Gly Val Ala Lys Lys Ala Lys Leu Tyr Gly Val Lys Val Leu Asp Asn
195 200 205
Gln Gly Ser Gly Ser Tyr Ser Gly Ile Ile Ser Gly Met Asp Tyr Val
210 215 220
Ala Gln Asp Ser Lys Thr Arg Gly Cys Pro Asn Gly Ala Ile Ala Ser
225 230 235 240
Met Ser Leu Gly Gly Gly Tyr Ser Ala Ser Val Asn Gln Gly Ala Ala
245 250 255
Ala Leu Val Asn Ser Gly Val Phe Leu Ala Val Ala Ala Gly Asn Asp
260 265 270
Asn Arg Asp Ala Gln Asn Thr Ser Pro Ala Ser Glu Pro Ser Ala Cys
275 280 285
Thr Val Gly Ala Ser Ala Glu Asn Asp Ser Arg Ser Ser Phe Ser Asn
290 295 300
Tyr Gly Arg Val Val Asp Ile Phe Ala Pro Gly Ser Asn Val Leu Ser
305 310 315 320
Thr Trp Ile Gly Gly Arg Thr Asn Thr Ile Ser Gly Thr Ser Met Ala
325 330 335
Thr Pro His Ile Ala Gly Leu Ala Ala Tyr Leu Ser Ala Leu Gln Gly
340 345 350
Lys Thr Thr Pro Ala Ala Leu Cys Lys Lys Ile Gln Asp Thr Ala Thr
355 360 365
Lys Asn Val Leu Thr Gly Val Pro Ser Gly Thr Val Asn Tyr Leu Ala
370 375 380
Tyr Asn Gly Ala
385
<210> 172
<211> 389
<212> PRT
<213> 洛斯里被毛孢
<400> 172
Met Lys Leu Ser Leu Ile Leu Ala Ile Leu Pro Ala Val Leu Ala Ala
1 5 10 15
Pro Ala Thr Lys Arg Asp Glu Pro Ala Pro Leu Leu Ile Pro Arg Gly
20 25 30
Ser Lys Gln Leu Ile Ala Asp Lys Tyr Ile Val Lys Phe Lys Asp Ser
35 40 45
Met Ser Ile Ala Ala Val Asp Lys Thr Val Lys Ala Leu Ser Asn Lys
50 55 60
Ala Asp His Val Tyr Thr Asn Thr Phe Arg Gly Phe Ala Gly Gln Leu
65 70 75 80
Ser Ala Lys Asp Val Lys Ala Leu Arg Glu Arg Pro Asp Val Glu Tyr
85 90 95
Val Glu Gln Asp Ser Ile Val Thr Ile Asn Ala Leu Lys Gln Gln Pro
100 105 110
Gly Ala Pro Trp Gly Leu Gly Arg Ile Ser His Arg Glu Pro Gly Ser
115 120 125
Thr Thr Tyr Val Tyr Asp Ser Ser Ala Gly Gln Gly Thr Cys Ala Tyr
130 135 140
Val Ile Asp Thr Gly Val Glu Ala Ser His Pro Glu Phe Glu Gly Arg
145 150 155 160
Ala Thr Met Val Arg Ser Phe Ile Asn Gly Gln Ala Thr Asp Gly His
165 170 175
Gly His Gly Thr His Cys Ala Gly Thr Ile Gly Ser Lys Thr Tyr Gly
180 185 190
Val Ala Lys Lys Thr Lys Ile Tyr Gly Val Lys Val Leu Ser Asn Gln
195 200 205
Gly Ser Gly Ala Val Ser Val Ile Ile Ala Gly Met Asp Phe Ala Val
210 215 220
Gln Asp Ser Arg Lys Arg Ser Cys Pro Lys Gly Val Val Ala Asn Met
225 230 235 240
Ser Leu Gly Gly Gly Phe Ser Ala Ala Ile Asn Asn Ala Ala Ala Ser
245 250 255
Met Ile Arg Ser Gly Val Phe Leu Ala Val Ala Ala Gly Asn Asp Asn
260 265 270
Thr Asp Ala Ser Gly Ser Ser Pro Ala Ser Glu Pro Thr Val Cys Thr
275 280 285
Val Gly Ala Ser Thr Ile Ser Asp Gln Arg Ser Ser Phe Ser Asn Tyr
290 295 300
Gly Ser Val Val Asp Ile Phe Ala Pro Gly Ser Asp Ile Leu Ser Thr
305 310 315 320
Trp Ile Gly Gly Arg Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Thr
325 330 335
Pro His Ile Ala Gly Leu Gly Ala Tyr Leu Ala Gly Leu Glu Gly Phe
340 345 350
Ser Asp Pro Gln Ala Leu Cys Ser Arg Ile Gln Glu Leu Ala Thr Ser
355 360 365
Asp Val Leu Thr Asp Ile Pro Asp Gly Thr Val Asn Lys Leu Ala Phe
370 375 380
Asn Gly Ala Ser Gln
385
<210> 173
<211> 389
<212> PRT
<213> Tolypocladium inflatum
<400> 173
Met Arg Leu Ser Ile Leu Leu Ser Ile Leu Pro Met Val Leu Ala Ala
1 5 10 15
Pro Ala Ala Lys Arg Asp Glu Pro Ala Pro Leu Leu Val Pro Arg Gly
20 25 30
Asn His Glu Leu Ile Ala Asp Lys Tyr Ile Val Lys Phe Lys Glu Gly
35 40 45
Ser Ala Leu Ser Val Leu Glu Asp Thr Met Ser Ser Leu Thr Gly Asn
50 55 60
Ala Asp His Val Tyr Asp Val Val Phe Lys Gly Phe Ala Gly His Leu
65 70 75 80
Asp Lys Val Met Leu Asp Val Leu Arg Gly His Pro Asp Val Glu Tyr
85 90 95
Ile Glu Gln Asp Ala Met Val Thr Ile Asn Gly Phe Val Ser Gln Pro
100 105 110
Gly Ala Thr Trp Gly Leu Gly Arg Val Ser His Arg Gln Pro Gly Ala
115 120 125
Thr Ser Tyr Asp His Asp Ser Ser Ala Gly Ala Gly Thr Cys Ala Tyr
130 135 140
Val Ile Asp Thr Gly Val Glu Ala Ser His Pro Glu Phe Glu Gly Arg
145 150 155 160
Ala Ala Gln Val Lys Ser Phe Ile Asn Gly Gln Asn Ser Asp Gly Asn
165 170 175
Gly His Gly Thr His Val Ser Gly Thr Ile Gly Ser Lys Ser Tyr Gly
180 185 190
Ile Ala Lys Lys Thr Lys Ile Tyr Gly Val Lys Val Leu Asp Asn Gln
195 200 205
Gly Ser Gly Ser Phe Ser Ser Ile Ile Ala Gly Met Asp Phe Val Ala
210 215 220
Lys Asp Ala Lys Thr Arg Gly Cys Pro Lys Gly Ala Val Ala Asn Met
225 230 235 240
Ser Leu Gly Gly Gly Tyr Ala Ala Ser Val Asn Gln Ala Ala Ala Ala
245 250 255
Leu Val Asn Ser Gly Val Phe Leu Gly Val Ala Ala Gly Asn Ser Asn
260 265 270
Thr Asp Ala Gly Ser Thr Ser Pro Ala Ser Glu Thr Ser Ala Cys Thr
275 280 285
Val Gly Ala Thr Asp Arg Asn Asp Asn Arg Ser Ser Phe Ser Asn Tyr
290 295 300
Gly Ser Val Val Asp Ile Phe Gly Pro Gly Ser Asp Ile Leu Ser Thr
305 310 315 320
Trp Ile Gly Gly Arg Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Thr
325 330 335
Pro His Ile Val Gly Leu Ala Ala Tyr Ile Gly Gly Leu Glu Gly Arg
340 345 350
Thr Gly Gly Ala Val Cys Asn Arg Leu Gln Glu Leu Ala Thr Arg Asn
355 360 365
Ala Ile Arg Gly Val Pro Ser Gly Thr Lys Asn Leu Leu Ala Phe Asn
370 375 380
Gly Asn Pro Ser Gly
385
<210> 174
<211> 387
<212> PRT
<213> Metacordyceps chlamydosporia
<400> 174
Met Gln Leu Ser Val Leu Leu Thr Leu Leu Pro Ala Val Leu Ala Ala
1 5 10 15
Pro Ala Ile Val Glu Gln Arg Ala Glu Pro Ala Pro Leu Phe Thr Pro
20 25 30
Lys Ser Ser Ile Ile Ala Gly Lys Tyr Ile Val Lys Phe Lys Asp Gly
35 40 45
Val Ala Arg Ile Ala Ala Asp Glu Ala Thr Ser Ala Leu Ser Ala Lys
50 55 60
Ala Asp His Val Tyr Ser His Leu Phe Asn Gly Phe Ala Gly Ser Leu
65 70 75 80
Thr Lys Glu Glu Leu Gln Thr Leu Arg Asn His Pro Asp Val Asp Phe
85 90 95
Ile Glu Lys Asp Ala Val Met Thr Ala Asn Ala Ile Val Glu Gln Gln
100 105 110
Gly Ala Pro Trp Gly Leu Gly Arg Ile Ser Asn Arg Gln Lys Gly Ser
115 120 125
Thr Thr Tyr Arg Tyr Asp Asp Ser Ala Gly Asn Gly Ala Cys Val Tyr
130 135 140
Val Leu Asp Thr Gly Ile Glu Thr Thr His Pro Glu Phe Glu Gly Arg
145 150 155 160
Ala Thr Trp Leu Lys Ser Phe Ile Asp Gly Glu Asn Asn Asp Gly His
165 170 175
Gly His Gly Thr His Cys Ala Gly Thr Val Gly Ser Lys Thr Tyr Gly
180 185 190
Val Ala Lys Lys Ala Lys Leu Leu Ala Val Lys Val Leu Asp Asn Gly
195 200 205
Gly Ser Gly Ser Tyr Ala Gly Val Ile Ala Gly Met Glu Phe Val Ser
210 215 220
Gln Asp Tyr Lys Thr Arg Gly Cys Pro Asn Gly Ala Ile Ala Ser Met
225 230 235 240
Ser Leu Gly Gly Pro Phe Ser Ala Ser Val Asn Gln Ala Ala Ala Ala
245 250 255
Met Val Ser Ser Gly Val Phe Leu Ser Val Ala Ala Gly Asn Asp Gly
260 265 270
Ala Asp Ala Ala Arg Tyr Ser Pro Ala Ser Glu Pro Ser Ala Cys Thr
275 280 285
Val Gly Ala Thr Thr Ser Thr Asp Ala Arg Ser Ser Phe Ser Asn Phe
290 295 300
Gly Lys Leu Val Asp Ile Phe Ala Pro Gly Ser Ala Ile Leu Ser Thr
305 310 315 320
Trp Ile Asn Gly Gly Thr Arg Ser Ile Ser Gly Thr Ser Met Ala Thr
325 330 335
Pro His Val Ala Gly Leu Ala Ala Tyr Leu Asn Ala Leu Gln Gly Val
340 345 350
Val Ser Pro Ala Ala Leu Cys Lys Lys Ile Gln Asp Thr Ala Ile Lys
355 360 365
Asn Ala Leu Thr Gly Val Pro Ala Ser Thr Val Asn Phe Leu Ala Tyr
370 375 380
Asn Gly Ala
385
<210> 175
<211> 387
<212> PRT
<213> 蛹虫草
<400> 175
Met Arg Leu Ser Leu Leu Leu Ala Ile Leu Pro Val Ala Leu Ala Ala
1 5 10 15
Pro Ala Thr Lys Arg Asp Glu Pro Ala Pro Leu His Ala Ala Ala Val
20 25 30
Glu Ser Ala Ala Ile Pro Gly Lys Tyr Ile Val Lys Phe Lys Lys Gly
35 40 45
Ser Ala Ser Ala Asp Asn Arg Met His Ile Met Ala Glu His Ala Glu
50 55 60
His Val Tyr Thr Asn Val Met Gln Gly Phe Ala Gly Ser Leu Asp Ala
65 70 75 80
Glu Thr Leu Lys Ala Leu Arg Asn His Pro Asp Val Glu Tyr Ile Glu
85 90 95
Gln Asp Ala Arg Val Ser Ile Asn Gly Trp Val Thr Gln Pro Gln Ser
100 105 110
Thr Trp Gly Leu Ser Arg Ile Ser His Arg Ala Lys Thr Ala Lys Gly
115 120 125
Tyr Thr Tyr Asp Ser Ser Ser Gly Glu Gly Thr Cys Val Tyr Val Ile
130 135 140
Asp Thr Gly Ile Asp Asp Ser His Pro Asp Phe Gly Gly Arg Ala Leu
145 150 155 160
Gln Val Lys Ser Phe Val Asp Gly Glu Pro Ala Asp Gly His Gly His
165 170 175
Gly Thr His Cys Ala Gly Thr Ile Ala Ser Asn Thr Tyr Gly Val Ala
180 185 190
Lys Lys Thr Lys Val Phe Gly Val Lys Val Leu Asp Asn Asn Gly Ser
195 200 205
Gly Ser Trp Ser Asn Val Ile Ala Gly Met Asp Phe Val Val Asn Asp
210 215 220
Ser Arg Glu Arg Asp Cys Pro Ser Gly Thr Leu Ala Ser Met Ser Leu
225 230 235 240
Gly Gly Gly Tyr Ser Ala Ala Ile Asn Gly Gly Ala Ala Glu Leu Val
245 250 255
Ser Ser Gly Val Phe Val Ser Val Ala Ala Gly Asn Asp Asn Arg Asp
260 265 270
Ala Ala Asn Tyr Ser Pro Ala Ser Glu Glu Ser Ala Cys Thr Val Gly
275 280 285
Ala Thr Thr Ser Ala Asp Ala Arg Ser Ala Phe Ser Asn Tyr Gly Arg
290 295 300
Ile Val Asp Ile Phe Ala Pro Gly Ser Asn Ile Leu Ser Leu Ala Pro
305 310 315 320
Gly Gly Gly Thr Ala Thr Met Ser Gly Thr Ser Met Ala Ala Pro His
325 330 335
Ile Ala Gly Leu Ala Ala Tyr Ile Gly Gly Leu Glu Gly Leu Thr Gly
340 345 350
Gly Ser Ile Cys Asn Arg Leu Val Gln Leu Ala Thr Arg Gly Thr Leu
355 360 365
Ala Gly Ile Pro Gly Gly Thr Val Asn Leu Leu Ala Phe Asn Gly Asn
370 375 380
Pro Ser Gly
385
<210> 176
<211> 379
<212> PRT
<213> 镰孢菌属
<400> 176
Met Arg Leu Ser Ile Ile Ala Val Leu Pro Leu Ala Leu Ala Ala Pro
1 5 10 15
Val Leu Glu Pro Ala Pro Leu Leu Glu Ala Arg Gly Ser Gln Pro Ile
20 25 30
Ala Gly Lys Tyr Ile Val Lys Leu Lys Asp Thr Ala Lys Ile Gly Ile
35 40 45
Met Glu Ala Thr Ala Lys Val Ala Asn Pro Glu Arg Val Tyr Gln Asn
50 55 60
Val Ile Lys Gly Phe Ser Ala Ser Leu Cys Lys Glu Glu Val Glu Arg
65 70 75 80
Leu Arg His Asp Pro Asp Val Glu Ser Ile Glu Gln Asp Ala Ile Ile
85 90 95
Ser Ile Asn Ala Ile Thr Gln Gln Gln Gly Ala Thr Trp Gly Leu Thr
100 105 110
Arg Ile Ser His Arg Gln Arg Gly Ser Thr Ala Tyr Ala Tyr Asp Thr
115 120 125
Thr Ala Gly Gln Gly Ala Cys Ala Tyr Val Ile Asp Thr Gly Val Glu
130 135 140
Asp Thr His Pro Glu Phe Glu Gly Arg Ala Lys Gln Ile Lys Thr Phe
145 150 155 160
Ala Ser Thr Ala Arg Asp Gly Asn Gly His Gly Thr His Cys Ser Gly
165 170 175
Thr Ile Gly Ser Lys Thr Tyr Gly Val Ala Lys Lys Val Ser Ile Phe
180 185 190
Gly Val Lys Val Leu Asp Asp Asn Gly Ser Gly Ser Leu Ser Asn Val
195 200 205
Ile Ala Gly Met Asp Phe Val Ala Ser Asp Tyr Arg Ser Arg Asn Cys
210 215 220
Pro Arg Gly Val Val Ala Ser Met Ser Leu Gly Gly Gly Tyr Ser Ala
225 230 235 240
Thr Val Asn Gln Ala Ala Ala Arg Leu Gln Ser Ser Gly Val Phe Val
245 250 255
Ala Val Ala Ala Gly Asn Asp Asn Arg Asp Ala Ala Asn Thr Ser Pro
260 265 270
Ala Ser Glu Pro Ser Val Cys Thr Val Gly Ala Thr Asp Ser Ser Asp
275 280 285
Arg Arg Ser Ser Phe Ser Asn Tyr Gly Arg Ala Leu Asp Ile Phe Ala
290 295 300
Pro Gly Thr Asp Ile Thr Ser Thr Trp Ile Gly Gly Arg Thr Asn Thr
305 310 315 320
Ile Ser Gly Thr Ser Met Ala Thr Pro His Ile Ala Gly Leu Gly Ala
325 330 335
Tyr Leu Leu Ala Leu Glu Gly Gly Ser Ala Ser Thr Ile Cys Ala Arg
340 345 350
Ile Gln Thr Leu Ser Thr Lys Asn Ala Ile Ser Gly Val Pro Ser Gly
355 360 365
Thr Val Asn Tyr Leu Ala Phe Asn Asn Ala Thr
370 375
<210> 177
<211> 383
<212> PRT
<213> 四孢链孢菌
<400> 177
Met Ala Ala Pro Ser Ala Pro Ile Asp Lys Arg Ala Pro Ile Leu Glu
1 5 10 15
Ala Arg Ala Gly Thr Gln Ala Val Pro Gly Lys Tyr Ile Val Lys Leu
20 25 30
Arg Glu Thr Ala Ser Asp Asp Asp Leu Asp Lys Ala Val Lys Lys Leu
35 40 45
Gly Asn Ser Lys Ala Asp His Val Tyr Lys His Ala Phe Arg Gly Phe
50 55 60
Ala Gly Arg Ile Asp Asp Lys Thr Leu Asp Glu Ile Arg Ser Ile Pro
65 70 75 80
Glu Val Glu Tyr Val Glu Gln Glu Ala Val Phe Thr Ile Asn Thr Tyr
85 90 95
Thr Ser Gln Ser Ser Val Pro Ser Trp Gly Leu Ala Arg Leu Ser Ser
100 105 110
Lys Thr Lys Gly Lys Thr Thr Tyr Val Tyr Asp Ser Ser Ala Gly Ala
115 120 125
Gly Thr Cys Ala Tyr Ile Ile Asp Thr Gly Ile Asn Thr Ala His Ser
130 135 140
Asp Phe Gly Gly Arg Ala Thr Trp Leu Ala Asn Tyr Ala Gly Asp Gly
145 150 155 160
Ile Asn Ser Asp Gly Asn Gly His Gly Thr His Val Ala Gly Thr Val
165 170 175
Gly Gly Thr Thr Tyr Gly Val Ala Lys Lys Thr Asn Leu Tyr Ala Val
180 185 190
Lys Val Leu Asp Ser Asn Gly Ser Gly Ser Asn Ser Gly Val Ile Ala
195 200 205
Gly Met Asn Phe Val Ala Gln Asp Ala Gln Ser Arg Asn Cys Pro Asn
210 215 220
Gly Thr Val Ala Asn Met Ser Leu Gly Gly Gly Tyr Ser Ala Ser Thr
225 230 235 240
Asn Ser Ala Ala Ala Ala Met Val Arg Ala Gly Val Phe Leu Ala Val
245 250 255
Ala Ala Gly Asn Asp Gly Ala Asn Ala Ala Asn Tyr Ser Pro Ala Ser
260 265 270
Glu Pro Thr Val Cys Thr Val Gly Ala Thr Thr Ser Ala Asp Ala Ile
275 280 285
Ala Tyr Tyr Ser Asn Tyr Gly Thr Ile Val Asp Ile Phe Ala Pro Gly
290 295 300
Thr Ser Ile Thr Ser Ala Trp Ile Gly Ser Thr Thr Ala Lys Asn Thr
305 310 315 320
Ile Ser Gly Thr Ser Met Ala Thr Pro His Ile Thr Gly Leu Gly Ala
325 330 335
Tyr Leu Leu Thr Leu Leu Gly Lys Lys Ser Pro Ala Ala Leu Cys Ser
340 345 350
Tyr Ile Ala Ser Thr Ala Asn Ser Gly Val Ile Ser Gly Ile Pro Ser
355 360 365
Gly Thr Val Asn Lys Leu Ala Phe Asn Gly Asn Pro Ser Ala Ser
370 375 380
<210> 178
<211> 393
<212> PRT
<213> 球毛壳霉
<400> 178
Met His Phe Ser Val Ala Leu Leu Ala Leu Leu Pro Ala Ala Ile Ala
1 5 10 15
Ala Pro Thr Ala Glu Pro Ile Gln Lys Arg Ala Pro Ile Ile Ala Ala
20 25 30
Arg Val Gly Gln Val Val Pro Asn Lys Tyr Leu Ile Lys Leu Arg Glu
35 40 45
Gly Thr Ser Asp Asp Ala Leu Glu Ala Ala Ile Gly Lys Leu Gly Lys
50 55 60
Ser Lys Ala Asp His Ile Tyr Arg Gly Lys Lys Phe Arg Gly Phe Ala
65 70 75 80
Gly Lys Leu Glu Ala Asp Leu Leu Asp Gln Ile Arg Leu Leu Pro Glu
85 90 95
Val Glu Tyr Val Glu Glu Asp Ala Ile Phe Ser Ile Asn Ala Tyr Val
100 105 110
Ser Gln Ser Gly Ala Pro Trp Gly Ile Ser Arg Leu Ser His Lys Ser
115 120 125
Ala Gly Ser Thr Thr Tyr Val Tyr Asp Ser Ser Ala Gly Glu Gly Thr
130 135 140
Cys Ser Tyr Val Ile Asp Thr Gly Ile Tyr Thr Ala His Ser Asp Phe
145 150 155 160
Gly Gly Arg Ala Thr Phe Ala Ala Asn Phe Val Asp Ser Ser Asn Thr
165 170 175
Asp Gly Asn Gly His Gly Thr His Val Ala Gly Thr Ile Gly Ser Thr
180 185 190
Thr Tyr Gly Val Ala Lys Lys Thr Lys Leu Tyr Ala Val Lys Val Leu
195 200 205
Gly Ser Asp Gly Ser Gly Ser Thr Ser Gly Val Val Ala Gly Ile Asn
210 215 220
Phe Val Ala Thr Asp Ala Pro Lys Arg Ser Cys Pro Lys Gly Val Val
225 230 235 240
Ala Asn Met Ser Leu Gly Gly Gly Tyr Ser Ala Ser Ile Asn Gln Ala
245 250 255
Ala Ala Ala Leu Val Asp Ala Gly Ile Phe Leu Ala Val Ala Ala Gly
260 265 270
Asn Asp Asn Ala Asn Ser Ala Asn Tyr Ser Pro Ala Ser Glu Ala Ser
275 280 285
Val Cys Thr Val Gly Ala Thr Asp Ser Ser Asp Arg Lys Ala Ser Tyr
290 295 300
Ser Asn Tyr Gly Ser Val Val Asp Ile Gln Ala Pro Gly Thr Asn Ile
305 310 315 320
Leu Ser Thr Trp Ile Gly Gly Arg Thr Asn Thr Ile Ser Gly Thr Ser
325 330 335
Met Ala Ser Pro His Ile Ala Gly Leu Ala Ala Tyr Leu Leu Ala Leu
340 345 350
Glu Gly Ser Lys Thr Pro Ala Ala Leu Cys Asn Tyr Ile Lys Ser Thr
355 360 365
Ala Leu Ser Gly Ala Val Ser Gly Leu Pro Ser Gly Thr Thr Asn Arg
370 375 380
Leu Ala Phe Asn Gly Asn Pro Ser Ala
385 390
<210> 179
<211> 396
<212> PRT
<213> 粗糙链孢霉
<400> 179
Met Lys Leu Ser Ala Val Leu Ala Leu Leu Pro Leu Ala Met Ala Ala
1 5 10 15
Pro Ser Ala Pro Ile Asp Lys Arg Ala Pro Ile Leu Glu Ala Arg Ala
20 25 30
Gly Thr Gln Ala Val Pro Gly Lys Tyr Ile Val Lys Leu Arg Glu Thr
35 40 45
Ala Ser Asp Asp Asp Leu Asp Lys Ala Val Lys Lys Leu Gly Asn Ser
50 55 60
Lys Ala Asp His Val Tyr Lys His Ala Phe Arg Gly Phe Ala Gly Arg
65 70 75 80
Ile Asp Asp Lys Thr Leu Asp Asp Ile Arg Ser Leu Pro Glu Val Glu
85 90 95
Tyr Val Glu Gln Glu Ala Val Phe Thr Ile Asn Thr Tyr Thr Ser Gln
100 105 110
Ser Ser Val Pro Ser Trp Gly Leu Ala Arg Leu Ser Ser Lys Thr Thr
115 120 125
Gly Lys Thr Thr Tyr Val Tyr Asp Ser Ser Ala Gly Ala Gly Thr Cys
130 135 140
Ala Tyr Ile Ile Asp Thr Gly Ile Asn Thr Ala His Ser Asp Phe Gly
145 150 155 160
Gly Arg Ala Thr Trp Leu Ala Asn Tyr Ala Gly Asp Gly Ile Asn Ser
165 170 175
Asp Gly Asn Gly His Gly Thr His Val Ala Gly Thr Val Gly Gly Thr
180 185 190
Thr Tyr Gly Val Ala Lys Lys Thr Gln Leu Tyr Ala Val Lys Val Leu
195 200 205
Asp Ser Asn Gly Ser Gly Ser Asn Ser Gly Val Ile Ala Gly Met Asn
210 215 220
Phe Val Ala Gln Asp Ala Gln Ser Arg Asn Cys Pro Asn Gly Thr Val
225 230 235 240
Ala Asn Met Ser Leu Gly Gly Gly Tyr Ser Ala Ser Thr Asn Ser Ala
245 250 255
Ala Ala Ala Met Val Arg Ala Gly Val Phe Leu Ala Val Ala Ala Gly
260 265 270
Asn Asp Gly Ala Asn Ala Ala Asn Tyr Ser Pro Ala Ser Glu Pro Thr
275 280 285
Val Cys Thr Val Gly Ala Thr Thr Ser Ala Asp Ala Ile Ala Tyr Tyr
290 295 300
Ser Asn Tyr Gly Thr Ile Val Asp Ile Phe Ala Pro Gly Thr Ser Ile
305 310 315 320
Thr Ser Ala Trp Ile Gly Ser Thr Thr Ala Lys Asn Thr Ile Ser Gly
325 330 335
Thr Ser Met Ala Thr Pro His Ile Thr Gly Leu Gly Ala Tyr Leu Leu
340 345 350
Thr Leu Leu Gly Lys Lys Ser Pro Ala Ala Leu Cys Ser Tyr Ile Ala
355 360 365
Ser Thr Ala Asn Ser Gly Val Ile Ser Gly Ile Pro Arg Gly Thr Val
370 375 380
Asn Lys Leu Ala Phe Asn Gly Asn Pro Ser Ala Tyr
385 390 395
<210> 180
<211> 397
<212> PRT
<213> 尖孢镰刀菌
<400> 180
Met Arg Ser Ala Thr Leu Leu Ala Leu Leu Pro Phe Ala Leu Ala Ala
1 5 10 15
Pro Ser Arg Arg Ala Glu Pro Ala Pro Ile Leu Arg Pro Arg Gly Val
20 25 30
Lys Leu Val Asp Gly Lys Tyr Ile Val Lys Met Lys Asn Gly Phe Gln
35 40 45
Ala Ser Ser Ile Glu Ser Trp Val Asp Lys Met Ile Asp Ser Ile Glu
50 55 60
Ala Asp Ala Asp Tyr Thr Tyr Ser Lys Gly Phe Gly Gly Phe Ala Ala
65 70 75 80
Ser Leu Lys Asp Asp Glu Leu Asn Lys Leu Lys His Asp Pro Asn Val
85 90 95
Glu Tyr Ile Glu Gln Asp Ala Tyr Ile Ser Ile Thr Ala Thr Thr Gln
100 105 110
Gln Ser Asn Ala Pro Trp Gly Ile Ala Arg Val Ser Ser Gln Ser Pro
115 120 125
Gly Gly Ser Thr Tyr Thr Tyr Asp Asp Ser Ala Gly Glu Gly Thr Cys
130 135 140
Ala Tyr Val Ile Asp Thr Gly Ile Asp Val Asp His Pro Asp Phe Asp
145 150 155 160
Gly Arg Ala Lys Phe Leu Lys Asn Phe Ala Gly Gly Ser Asp Ser Asp
165 170 175
Gly Gln Gly His Gly Thr His Val Ala Gly Thr Ile Gly Ser Thr Thr
180 185 190
Tyr Gly Val Ala Lys Lys Thr Ser Leu Phe Ala Val Lys Val Leu Gly
195 200 205
Asp Asp Gly Ser Gly Thr Asn Ser Ala Val Ile Ala Gly Met Asp Phe
210 215 220
Val Ser Gly His Ala Lys Asp Glu Asn Cys Pro Asn Gly Val Val Val
225 230 235 240
Asn Met Ser Leu Gly Gly Glu Thr Ser Asp Ala Val Asn Gln Ala Ala
245 250 255
Lys Ala Ile Val Asp Ala Gly Leu Phe Leu Ala Val Ala Ala Gly Asn
260 265 270
Asp Gly Lys Asp Ala Ser Gly Ser Ser Pro Ala Ser Glu Glu Ser Ala
275 280 285
Cys Thr Val Gly Ala Thr Thr Arg Asp Asp Thr Leu Ala Asp Tyr Ser
290 295 300
Asn Phe Gly Ser Val Val Asp Val Leu Ala Pro Gly Thr Asp Ile Leu
305 310 315 320
Ser Thr Trp Pro Asn Gly Lys Thr Asn Thr Ile Ser Gly Thr Ser Met
325 330 335
Ala Ser Pro His Val Ala Gly Leu Ala Ala Tyr Phe Leu Gly Leu Gly
340 345 350
Gln Lys Ala Glu Gly Leu Cys Asp Tyr Ile Ala Ser Lys Ala Leu Asp
355 360 365
Gly Val Ile Ser Asn Val Pro Ser Gly Thr Val Asn Lys Leu Ile Asn
370 375 380
Asn Gly Val Gly Gly Ser Asn Ser Ser Ser Ile Arg His
385 390 395
<210> 181
<211> 389
<212> PRT
<213> 玉米赤霉
<400> 181
Met Arg Ser Ala Thr Leu Leu Ala Leu Leu Pro Phe Ala Leu Ala Ala
1 5 10 15
Pro Ser Ala Ser Val Ser Arg Arg Thr Glu Pro Ala Pro Val Ile Arg
20 25 30
Pro Arg Gly Val Lys Leu Val Asp Gly Lys Tyr Ile Val Lys Met Lys
35 40 45
Ala Gly Val Arg Ala Ala Ser Val Asp Ser Ala Val Ser Thr Ile Gln
50 55 60
Ala Asp Ala Asp Tyr Thr Tyr Thr Lys Ser Phe Ser Gly Phe Ala Ala
65 70 75 80
Ser Leu Lys Asp Glu Glu Ile Glu Thr Leu Lys His Asp Pro Asn Val
85 90 95
Glu Tyr Ile Glu Gln Asp Ala Val Ile Thr Ile Lys Ala Thr Val Asp
100 105 110
Gln Asp Asn Ala Pro Trp Gly Ile Ala Arg Leu Ser Ser Ser Lys Pro
115 120 125
Gly Ser Lys Thr Tyr Thr Tyr Asp Glu Ser Ala Gly Glu Gly Thr Cys
130 135 140
Ser Tyr Val Ile Asp Thr Gly Ile Asp Val Glu His Pro Asp Phe Asp
145 150 155 160
Gly Arg Ala Lys Phe Leu Lys Asn Phe Ala Gly Gly Arg Asp Gly Asp
165 170 175
Gly Gln Gly His Gly Thr His Val Ala Gly Thr Ile Gly Ser Thr Thr
180 185 190
Tyr Gly Val Ala Lys Lys Thr Thr Leu Tyr Ala Val Lys Val Leu Gly
195 200 205
Asp Asp Gly Ser Gly Thr Asn Ser Ala Val Ile Ala Gly Met Asp Phe
210 215 220
Val Ala Gly His Ala Asp Asp Glu Asn Cys Pro Asn Gly Ala Val Val
225 230 235 240
Asn Met Ser Leu Gly Gly Glu Ala Ser Asp Ala Val Asn Ser Ala Ala
245 250 255
Lys Ser Ile Val Asp Ala Gly Leu Phe Leu Ala Val Ala Ala Gly Asn
260 265 270
Glu Ala Val Asp Ala Ser Gly Ser Ser Pro Ala Ser Glu Ala Ser Ala
275 280 285
Cys Thr Val Gly Ala Thr Thr Arg Asn Asp Thr Leu Ser Tyr Phe Ser
290 295 300
Asn Phe Gly Asp Leu Val Asp Val Leu Ala Pro Gly Thr Asp Ile Leu
305 310 315 320
Ser Thr Trp Pro Gly Gly Lys Thr Asn Thr Ile Ser Gly Thr Ser Met
325 330 335
Ala Ser Pro His Val Ala Gly Leu Gly Ala Tyr Phe Leu Gly Leu Gly
340 345 350
Lys Lys Ala Glu Gly Leu Cys Glu Tyr Ile Ala Ser Gln Ala Leu Glu
355 360 365
Gly Val Val Ala Gln Val Pro Arg Asp Thr Val Asn Lys Leu Ile Asn
370 375 380
Asn Gly Val Ser Lys
385
<210> 182
<211> 399
<212> PRT
<213> 里氏木霉
<400> 182
Met Gln Pro Ser Phe Gly Ser Phe Leu Val Thr Val Leu Ser Ala Ser
1 5 10 15
Met Ala Ala Gly Ser Val Ile Pro Ser Thr Asn Ala Asn Pro Gly Ser
20 25 30
Phe Glu Ile Lys Arg Ser Ala Asn Lys Ala Phe Thr Gly Arg Asn Gly
35 40 45
Pro Leu Ala Leu Ala Arg Thr Tyr Ala Lys Tyr Gly Val Glu Val Pro
50 55 60
Lys Thr Leu Val Asp Ala Ile Gln Leu Val Lys Ser Ile Gln Leu Ala
65 70 75 80
Lys Arg Asp Ser Ala Thr Val Thr Ala Thr Pro Asp His Asp Asp Ile
85 90 95
Glu Tyr Leu Val Pro Val Lys Ile Gly Thr Pro Pro Gln Thr Leu Asn
100 105 110
Leu Asp Phe Asp Thr Gly Ser Ser Asp Leu Trp Val Phe Ser Ser Asp
115 120 125
Val Asp Pro Thr Ser Ser Gln Gly His Asp Ile Tyr Thr Pro Ser Lys
130 135 140
Ser Thr Ser Ser Lys Lys Leu Glu Gly Ala Ser Trp Asn Ile Thr Tyr
145 150 155 160
Gly Asp Arg Ser Ser Ser Ser Gly Asp Val Tyr His Asp Ile Val Ser
165 170 175
Val Gly Asn Leu Thr Val Lys Ser Gln Ala Val Glu Ser Ala Arg Asn
180 185 190
Val Ser Ala Gln Phe Thr Gln Gly Asn Asn Asp Gly Leu Val Gly Leu
195 200 205
Ala Phe Ser Ser Ile Asn Thr Val Lys Pro Thr Pro Gln Lys Thr Trp
210 215 220
Tyr Asp Asn Ile Val Gly Ser Leu Asp Ser Pro Val Phe Val Ala Asp
225 230 235 240
Leu Arg His Asp Thr Pro Gly Ser Tyr His Phe Gly Ser Ile Pro Ser
245 250 255
Glu Ala Ser Lys Ala Phe Tyr Ala Pro Ile Asp Asn Ser Lys Gly Phe
260 265 270
Trp Gln Phe Ser Thr Ser Ser Asn Ile Ser Gly Gln Phe Asn Ala Val
275 280 285
Ala Asp Thr Gly Thr Thr Leu Leu Leu Ala Ser Asp Asp Leu Val Lys
290 295 300
Ala Tyr Tyr Ala Lys Val Gln Gly Ala Arg Val Asn Val Phe Leu Gly
305 310 315 320
Gly Tyr Val Phe Asn Cys Thr Thr Gln Leu Pro Asp Phe Thr Phe Thr
325 330 335
Val Gly Glu Gly Asn Ile Thr Val Pro Gly Thr Leu Ile Asn Tyr Ser
340 345 350
Glu Ala Gly Asn Gly Gln Cys Phe Gly Gly Ile Gln Pro Ser Gly Gly
355 360 365
Leu Pro Phe Ala Ile Phe Gly Asp Ile Ala Leu Lys Ala Ala Tyr Val
370 375 380
Ile Phe Asp Ser Gly Asn Lys Gln Val Gly Trp Ala Gln Lys Lys
385 390 395
<210> 183
<211> 401
<212> PRT
<213> 深绿木霉
<400> 183
Met Gln Pro Thr Thr Ser Phe Gly Thr Phe Leu Val Thr Phe Leu Ser
1 5 10 15
Ala Ser Met Ala Ala Gly Ser Ala Ile Pro Tyr Met Ala Pro Gln Pro
20 25 30
Gly Ser Phe Glu Ile Lys Arg Ala Ala Asn Asn Gly Phe Thr Gly Arg
35 40 45
Asn Gly Ala Leu Ala Leu Ala Arg Ala Tyr Ala Lys Tyr Gly Val Pro
50 55 60
Val Thr Asp Thr Leu Leu Glu Ala Val Glu Thr Leu Lys Ser Ala His
65 70 75 80
Leu Ile Lys Arg Ser Ser Gly Asn Val Thr Ala Thr Pro Asp Gly Asp
85 90 95
Asp Leu Glu Tyr Leu Val Pro Val Gln Ile Gly Thr Pro Pro Gln Thr
100 105 110
Leu Asn Leu Asp Phe Asp Thr Gly Ser Ser Asp Leu Trp Val Phe Ser
115 120 125
Ser Glu Thr Asp Thr Ser Ser Ser Gln Gly His Ser Val Tyr Ser Pro
130 135 140
Ser Lys Ser Thr Ser Ala Gln Lys Leu Asp Gly Ala Thr Trp Ser Ile
145 150 155 160
Thr Tyr Gly Asp Gln Ser Ser Ser Ser Gly Asp Val Tyr Thr Asp Val
165 170 175
Val Thr Leu Gly Ser Leu Thr Val Lys Ser Gln Ala Val Glu Ser Ala
180 185 190
Gln Gln Val Ser Ala Gln Phe Ala Gln Gly Asn Asn Asp Gly Leu Leu
195 200 205
Gly Leu Ala Phe Ser Ser Ile Asn Thr Val Lys Pro Thr Gln Gln Lys
210 215 220
Thr Trp Phe Asp Asn Ile Ser Gly Ser Leu Asp Ser Pro Leu Phe Val
225 230 235 240
Ala Asp Leu Arg His Glu Thr Pro Gly Ser Tyr Ile Phe Gly Thr Ile
245 250 255
Pro Ser Asp Ala Ser Asn Val Leu Tyr Ala Pro Val Asp Asn Ser Gln
260 265 270
Gly Phe Trp Gln Phe Ser Thr Ser Ser Asp Ile Gly Gly Ser Phe Ser
275 280 285
Ala Ile Ala Asp Thr Gly Thr Thr Leu Leu Leu Ala Ser Asp Asp Leu
290 295 300
Val Ser Ala Tyr Tyr Lys Asn Val Gln Gly Ala Arg Glu Asp Gln Gln
305 310 315 320
Gln Gly Gly Tyr Val Phe Asp Cys Ser Thr Thr Leu Pro Asp Phe Thr
325 330 335
Phe Thr Val Gly Asp Gly Lys Ile Thr Val Pro Gly Ser Leu Ile Asn
340 345 350
Tyr Gly Gln Ala Ser Gly Ser Glu Cys Phe Gly Gly Ile Gln Ser Ser
355 360 365
Gly Gly Leu Pro Phe Ala Ile Phe Gly Asp Ile Ala Leu Lys Ala Ala
370 375 380
Tyr Val Val Phe Asp Ser Gly Asn Thr Gln Val Gly Trp Ala Gln Lys
385 390 395 400
Lys
<210> 184
<211> 400
<212> PRT
<213> 绿木霉
<400> 184
Met Gln Pro Val Ser Phe Gly Ser Phe Leu Val Thr Phe Leu Thr Ala
1 5 10 15
Ser Leu Ala Ala Gly Ser Ala Leu Pro Asn Thr Val Ser Ser Pro Gly
20 25 30
Ser Phe Glu Ile Lys Arg Thr Pro Asn Lys Gly Phe Thr Gly Arg Asn
35 40 45
Gly Pro Leu Ala Leu Ala Arg Ala Tyr Val Lys Tyr Gly Val Pro Val
50 55 60
Ser Lys Ser Leu Val Glu Ala Ile Asp Leu Val Lys Ser Ala Gln Leu
65 70 75 80
Ile Lys Arg Ser Asn Gly Asn Val Thr Ala Thr Pro Asp Gly Asp Asp
85 90 95
Leu Gln Tyr Leu Val Pro Val Gln Ile Gly Thr Pro Pro Gln Thr Leu
100 105 110
His Leu Asp Phe Asp Thr Gly Ser Ser Asp Leu Trp Val Phe Ser Ser
115 120 125
Glu Thr Asp Pro Ser Ser Ser Lys Gly His Ser Leu Tyr Asn Pro Ser
130 135 140
Thr Ser Thr Ser Ser Lys Lys Val Asp Gly Ala Ser Trp Asn Ile Thr
145 150 155 160
Tyr Gly Asp Gln Ser Ser Ser Ser Gly Asp Val Tyr Thr Asp Val Val
165 170 175
Thr Val Gly Ser Leu Thr Val Asn Ser Gln Ala Val Glu Ser Ala Gln
180 185 190
Gln Val Ser Ala Gln Phe Ala Gln Gly Asn Asn Asp Gly Leu Val Gly
195 200 205
Leu Ala Phe Ser Ser Ile Asn Thr Val Lys Pro Thr Pro Gln Lys Thr
210 215 220
Trp Phe Asp Asn Ile Ala Ser Thr Leu Asp Ala Pro Leu Phe Val Ala
225 230 235 240
Asp Leu Arg His Glu Thr Pro Gly Ser Tyr Ile Phe Gly Ala Ile Pro
245 250 255
Ser Ala Ala Ser Asn Ile Leu Tyr Ala Pro Val Asp Asn Ser Lys Gly
260 265 270
Phe Trp Gln Phe Ser Thr Ser Ser Asp Thr Gly Gly Glu Phe Asp Ala
275 280 285
Ile Ala Asp Thr Gly Thr Thr Leu Ile Leu Ala Ser Asp Ala Leu Val
290 295 300
Lys Ser Tyr Tyr Gln Lys Val Gln Gly Ala His Leu Asp Ala Leu Gln
305 310 315 320
Gly Gly Tyr Val Phe Gly Cys Asn Thr Thr Leu Pro Asp Phe Lys Phe
325 330 335
Ala Val Gly Glu Gly Glu Ile Thr Val Pro Gly Ser Leu Ile Asn Tyr
340 345 350
Ala Pro Ala Gly Asn Asn Glu Cys Phe Gly Gly Ile Gln Pro Ser Gly
355 360 365
Gly Leu Pro Phe Ala Ile Phe Gly Asp Ile Ala Leu Lys Ala Ala His
370 375 380
Val Val Phe Asp Ser Gly Lys Thr Gln Val Gly Trp Ala Gln Lys Lys
385 390 395 400
<210> 185
<211> 398
<212> PRT
<213> 蛹虫草
<400> 185
Met Tyr Lys Asp Val Phe Pro Cys Ser Gly Phe Lys Ser Phe Gly Ser
1 5 10 15
Phe Leu Val Ala Ala Leu Val Ala Gly Phe Ala Ser Gly Asn Pro Met
20 25 30
Pro Arg Ala Ser Gly Ser Phe Glu Leu Lys Gln Thr Ala Asn Pro Asn
35 40 45
Phe Ser Ser Arg Asn Gly Pro Gln Ala Leu Ala Asn Ala Tyr Lys Lys
50 55 60
Tyr Gly Lys Ala Val Pro Asp His Ile Ala Val Ala Ala Asp Lys Asn
65 70 75 80
Thr Asn Ala Lys Arg Ala Ala Gly Thr Ala Thr Ala Asn Ser Ile Arg
85 90 95
Gly Asp Leu Glu Tyr Leu Val Asp Val Gln Ile Gly Ala Gly Gln Thr
100 105 110
Leu Lys Leu Asp Phe Asp Thr Gly Ser Ser Asp Leu Trp Val Phe Ser
115 120 125
Thr Gln Thr Glu Gly Ala Gly Phe Gln Thr Lys Tyr Asp Pro Ser Arg
130 135 140
Ser Ser Ser Ala Lys Arg Leu Ser Gly Ala Thr Trp Glu Ile Ser Tyr
145 150 155 160
Gly Asp Gly Ser Asn Cys Ser Gly Asp Val Tyr Thr Asp Asp Val Thr
165 170 175
Val Gly Gly Leu Thr Val Lys Gly Gln Ala Val Glu Ala Ala Gln Glu
180 185 190
Val Ser Gln Gln Phe Ala Gln Gly Gln Ser Asp Gly Leu Leu Gly Leu
195 200 205
Ala Phe Ser Gln Leu Asn Thr Val Thr Pro Gln Lys Gln Lys Thr Trp
210 215 220
Phe Asp Asn Val Lys Pro Gln Leu Asp Ser Pro Leu Phe Val Ala Asp
225 230 235 240
Leu Lys His Asn Ala Pro Gly Ser Tyr Ile Phe Gly Lys Ile Pro Ser
245 250 255
Gly Ala Glu Gln Ala Ser Tyr Val Asp Val Asp Ser Ser Gln Gly Phe
260 265 270
Trp Gly Phe Ser Ala Thr Thr Met Tyr Gly Asp Val Gln Ala Ile Ala
275 280 285
Asp Thr Gly Thr Thr Leu Leu Leu Leu Asp Asn Gln Ser Val Gln Ser
290 295 300
Tyr Tyr Ala Asn Val Gln Ser Ala Glu Tyr Asp Gln Gln Glu Gly Gly
305 310 315 320
Phe Val Phe Asn Cys Gly Glu Gln Leu Pro Asp Phe Pro Phe Arg Val
325 330 335
Gly Gly Gly Ile Ile Thr Ile Pro Gly Ala Ile Ile Lys Tyr Ala Gln
340 345 350
Gly Ser Asp Gly Arg Cys Phe Gly Gly Ile Gln Ala Ala Gly Asn Ile
355 360 365
Pro Leu Asn Ile Phe Gly Asp Ile Ala Leu Lys Ala Ala Tyr Val Ile
370 375 380
Phe Asp Gly Gly Asn Asn Arg Leu Gly Trp Ala Gln Lys Asn
385 390 395
<210> 186
<211> 446
<212> PRT
<213> 里氏木霉
<400> 186
Met Thr Leu Pro Val Pro Leu Arg Glu His Asp Leu Pro Phe Leu Lys
1 5 10 15
Glu Lys Arg Lys Leu Pro Ala Asp Asp Ile Pro Ser Gly Thr Tyr Thr
20 25 30
Leu Pro Ile Ile His Ala Arg Arg Pro Lys Leu Ala Ser Arg Ala Ile
35 40 45
Glu Val Gln Val Glu Asn Arg Ser Asp Val Ser Tyr Tyr Ala Gln Leu
50 55 60
Asn Ile Gly Thr Pro Pro Gln Thr Val Tyr Ala Gln Ile Asp Thr Gly
65 70 75 80
Ser Phe Glu Leu Trp Val Asn Pro Asn Cys Ser Asn Val Gln Ser Ala
85 90 95
Asp Gln Arg Phe Cys Arg Ala Ile Gly Phe Tyr Asp Pro Ser Ser Ser
100 105 110
Ser Thr Ala Asp Val Thr Ser Gln Ser Ala Arg Leu Arg Tyr Gly Ile
115 120 125
Gly Ser Ala Asp Val Thr Tyr Val His Asp Thr Ile Ser Leu Pro Gly
130 135 140
Ser Gly Ser Gly Ser Lys Ala Met Lys Ala Val Gln Phe Gly Val Ala
145 150 155 160
Asp Thr Ser Val Asp Glu Phe Ser Gly Ile Leu Gly Leu Gly Ala Gly
165 170 175
Asn Gly Ile Asn Thr Glu Tyr Pro Asn Phe Val Asp Glu Leu Ala Ala
180 185 190
Gln Gly Val Thr Ala Thr Lys Ala Phe Ser Leu Ala Leu Gly Ser Lys
195 200 205
Ala Glu Glu Glu Gly Val Ile Ile Phe Gly Gly Val Asp Thr Ala Lys
210 215 220
Phe His Gly Glu Leu Ala His Leu Pro Ile Val Pro Ala Asp Asp Ser
225 230 235 240
Pro Asp Gly Val Ala Arg Tyr Trp Val Lys Met Lys Ser Ile Ser Leu
245 250 255
Thr Pro Pro Pro Pro Ser Ser Ser Gly Ser Thr Asp Asp Asn Asn Asn
260 265 270
Lys Pro Val Ala Phe Pro Gln Thr Ser Met Thr Val Phe Leu Asp Ser
275 280 285
Gly Ser Thr Leu Thr Leu Leu Pro Pro Ala Leu Val Arg Gln Ile Ala
290 295 300
Ser Ala Leu Gly Ser Thr Gln Thr Asp Glu Ser Gly Phe Phe Val Val
305 310 315 320
Asp Cys Ala Leu Ala Ser Gln Asp Gly Thr Ile Asp Phe Glu Phe Asp
325 330 335
Gly Val Thr Ile Arg Val Pro Tyr Ala Glu Met Ile Arg Gln Val Ser
340 345 350
Thr Leu Pro Pro His Cys Tyr Leu Gly Met Met Gly Ser Thr Gln Phe
355 360 365
Ala Leu Leu Gly Asp Thr Phe Leu Arg Ser Ala Tyr Ala Val Phe Asp
370 375 380
Leu Thr Ser Asn Val Val His Leu Ala Pro Tyr Ala Asn Cys Gly Thr
385 390 395 400
Asn Val Lys Ser Ile Thr Ser Thr Ser Ser Leu Ser Asn Leu Val Gly
405 410 415
Thr Cys Asn Asp Pro Ser Lys Pro Ser Ser Ser Pro Ser Pro Ser Gln
420 425 430
Thr Pro Ser Ala Ser Pro Ser Ser Thr Ala Thr Gln Lys Ala
435 440 445
<210> 187
<211> 608
<212> PRT
<213> 深绿木霉
<400> 187
Met Ala Ile Arg Ser Phe Leu Thr Leu Ser Leu Ala Leu Leu Ala His
1 5 10 15
Ala Arg Pro Arg His Val Phe Asp Pro Glu Asp Val Gly Thr Lys Ile
20 25 30
Thr Leu Pro Val Ile Ser Ala Pro Arg Ala Val Glu Ala Val Ala Gly
35 40 45
Arg Ser Ala Gly Asp Asp Glu Asp Lys Pro Phe His Ile Leu Pro Phe
50 55 60
Pro Tyr Thr Gly Asn Gly Lys Arg Ala Ile Leu Glu Asp Asp Asp Gly
65 70 75 80
Ala Ala Gly Ala Val Thr Leu Pro Val Pro Arg Pro Ala Ser Arg Arg
85 90 95
Ser Ala Gly Asp Glu Asn Glu Asp Glu Glu Asp Lys Pro Phe His Ile
100 105 110
Leu Pro Phe Asn Glu Lys Arg Lys Leu Pro Val Glu Glu Gly Pro Ile
115 120 125
Gly Thr Ile Thr Leu Pro Val Ile His Ala Ala Arg Pro Val Leu Asp
130 135 140
Lys Arg Ala Val Glu Val Gln Val Glu Asn Arg Ser Asp Val Ser Tyr
145 150 155 160
Tyr Ala Gln Leu Asn Ile Gly Thr Pro Pro Gln Thr Val Tyr Ala Gln
165 170 175
Ile Asp Thr Gly Ser Phe Glu Leu Trp Val Asn Pro Asp Cys Ser Asn
180 185 190
Val Ala Ser Ser Asp Arg Arg Phe Cys Gln Ala Ile Gly Ser Tyr Asn
195 200 205
Pro Arg Ser Ser Ser Ser Ala Ala Thr Thr Asp Glu Ser Ala Met Leu
210 215 220
Arg Tyr Gly Ile Gly Ser Ala Asn Val Ser Tyr Phe Lys Asp Thr Ile
225 230 235 240
Ala Leu Ser Gly Ala Pro Ala Met Lys Gln Val Gln Phe Gly Val Ala
245 250 255
Ser Ala Ser Val Asp Glu Phe Ser Gly Ile Leu Gly Ile Gly Ala Gly
260 265 270
Glu Gly Val Asn Thr Asp Tyr Pro Asn Phe Ile Asp Ala Leu Ala Ala
275 280 285
Gln Gly Val Thr Lys Thr Lys Ala Phe Ser Leu Ala Leu Gly Ser Lys
290 295 300
Ala Glu Glu Glu Gly Val Ile Ile Phe Gly Gly Val Asp Thr Ala Lys
305 310 315 320
Phe Gln Gly Lys Leu Ala Thr Leu Pro Ile Val Pro Ala Lys Glu Ser
325 330 335
Pro Asp Gly Val Ala Arg Tyr Trp Val Arg Met Asn Ser Ile Ala Leu
340 345 350
Ser Pro Pro Gln Gly Lys Ala Thr Thr Phe Ser Gln Thr Asp Met Thr
355 360 365
Val Phe Leu Asp Ser Gly Ser Thr Leu Thr Leu Leu Pro Glu Gln Val
370 375 380
Val Glu Glu Ile Ala Thr Gly Leu Gly Ser Ser Gly Met Asp Ser Thr
385 390 395 400
Gly Tyr Tyr Ala Ile Asn Cys Asn Leu Ala Ser Gln Lys Gly Thr Ile
405 410 415
Asp Phe Ala Phe Asp Gly Val Thr Ile Ser Val Pro Tyr Ser Glu Val
420 425 430
Ile Arg Gln Val Ser Ser Ser Pro Pro Gln Cys Tyr Leu Gly Met Met
435 440 445
Gly Ser Thr Glu Phe Ala Leu Leu Gly Asp Thr Phe Leu Arg Ser Ala
450 455 460
Tyr Ala Val Phe Asp Leu Thr Gly Asn Ser Ile His Leu Ala Gln Tyr
465 470 475 480
Ala Asn Cys Gly Thr Lys Val Leu Thr Ile Thr Pro Asp Ser Ala Leu
485 490 495
Gln Gly Leu Ser Ser Ser Cys Gly Gly Gly Ser Glu Ser Pro Ala Asn
500 505 510
Pro Pro Ala Ser Ser Ser Ser Ser Ala Ala Pro Ala Ala Pro Ser Lys
515 520 525
Ser Ala Ala Ser Lys Pro Ser Ala Pro Ala Ser Ser Ser Val Gln Pro
530 535 540
Val His Ser Pro Thr Pro Ala Ala Ser Lys Pro Ala Asp Gln Thr Pro
545 550 555 560
Pro Ala Thr Thr Thr Ala Ala Ser Thr Pro Lys Ser Thr Pro Ser Ser
565 570 575
Val Ser Asp Ala Ala Gly Lys Ser Ser Ala Gly Arg Val Asn Val Gln
580 585 590
Ala Val Gly Trp Ser Met Val Ser Ala Ala Met Gly Leu Leu Leu Leu
595 600 605
<210> 188
<211> 655
<212> PRT
<213> 绿木霉
<400> 188
Met Gly Ile Ser Ser Phe Leu Val Leu Ser Leu Ala Val Leu Ala Gln
1 5 10 15
Ala Arg Pro Arg His Ala Phe Glu Pro Glu Asp Val Gly Asp Arg Ile
20 25 30
Thr Leu Pro Val Ile Ser Ala Arg Ala Val Asp Val Lys Gly Asp Leu
35 40 45
Ser Gln Arg Ser Ala Ala Asp Glu Glu Asp Lys Pro Phe His Ile Leu
50 55 60
Pro Phe Pro Tyr Pro Ser Lys Arg Asp Val Glu Ser Arg Glu Glu Glu
65 70 75 80
Asp Lys Pro Trp His Ile Leu Pro Phe Pro Tyr Pro Ser Lys Arg Asp
85 90 95
Val Glu Ser Arg Glu Glu Glu Asp Lys Pro Trp His Ile Leu Pro Phe
100 105 110
Pro Gly Pro Ser Lys Arg Lys Ile Leu Glu Asp Asp Asp Gly Ala Asp
115 120 125
Gly Arg Ile Thr Leu Pro Val Pro Gln Arg Ala Arg Arg Ser Glu Gly
130 135 140
Glu Glu Asp Lys Pro Phe His Ile Leu Pro Phe Asn Asp Lys Arg Lys
145 150 155 160
Leu Pro Val Glu Asp Val Pro Ser Gly Thr Ile Thr Leu Pro Ile Ile
165 170 175
His Arg His Arg Ser Phe Leu Asp Lys Arg Ala Val Glu Val Gln Val
180 185 190
Glu Asn Arg Ser Asp Val Ser Tyr Tyr Ala Gln Leu Asn Ile Gly Thr
195 200 205
Pro Pro Gln Thr Val Tyr Ala Gln Ile Asp Thr Gly Ser Phe Glu Leu
210 215 220
Trp Val Asn Pro Asp Cys Ser Asn Val Gln Thr Gly Asp Gln Arg Phe
225 230 235 240
Cys Arg Ala Ile Gly Val Tyr Asp Pro Lys Ser Ser Ser Thr Ser Phe
245 250 255
Val Thr Gly Ser Ala Ala Gln Leu Arg Tyr Gly Ile Gly Ser Ala Asn
260 265 270
Val Ser Tyr Val Gln Asp Ser Ile Ser Leu Pro Gly Ser Ala Thr Met
275 280 285
Lys Gln Val Gln Phe Gly Val Ala Leu Ser Ser Val Asp Glu Phe Ser
290 295 300
Gly Ile Leu Gly Ile Gly Ala Gly Asp Gly Phe Asn Thr Leu Tyr Pro
305 310 315 320
Asn Phe Ile Asp Glu Leu Ala Ser Gln Gly Val Thr Lys Thr Lys Ala
325 330 335
Phe Ser Leu Ala Leu Gly Ser Lys Ala Glu Glu Glu Gly Val Ile Ile
340 345 350
Phe Gly Gly Val Asp Thr Gly Lys Phe Thr Gly Lys Leu Ala Asn Leu
355 360 365
Pro Ile Val Pro Ala Asp Asp Ser Pro Asp Gly Val Ala Arg Tyr Trp
370 375 380
Val Gly Met Asn Ser Ile Ser Leu Ser Pro Pro Asn Gly Lys Ser Thr
385 390 395 400
Thr Phe Ala Gln Thr Lys Met Thr Val Phe Leu Asp Ser Gly Ser Thr
405 410 415
Leu Thr Leu Leu Pro Gln Ser Leu Val Asn Glu Ile Ala Asp Gly Leu
420 425 430
Gly Ser Thr Gly Thr Asp Asp Ser Gly Phe Ala Ile Val Asp Cys Asp
435 440 445
Leu Ala Asn Gln Pro Gly Thr Ile Asp Phe Ala Phe Asp Gly Val Thr
450 455 460
Ile Lys Val Pro Tyr Ser Glu Met Ile Arg Ser Val Asn Ser Leu Pro
465 470 475 480
Pro His Cys Tyr Leu Gly Met Met Gly Ser Thr Gln Phe Ala Leu Leu
485 490 495
Gly Asp Thr Phe Leu Arg Ser Ala Tyr Ala Val Phe Asp Leu Thr Gly
500 505 510
Asn Met Ile His Leu Ala Gln Tyr Val Asn Cys Gly Thr Asn Val Lys
515 520 525
Ser Ile Thr Ser Thr Ser Ser Leu Leu Gly Leu Ala Gly Ser Cys Ser
530 535 540
Ser Asp Gly Ser Glu Ser Pro Ser Ser Pro Ser Ser Ser Ser Thr Ser
545 550 555 560
Ser Ser Pro Ser Lys Thr Ala Ser Lys Ala Ala Pro Ser Ala Thr Ala
565 570 575
Pro Ser Ser Ser Thr Val Gln Pro Pro Ser Ser Ala Gln Ser Thr Ala
580 585 590
Ala Ser Pro Ser Ala Ala Arg Ser Ser Asp Ala Ala Ser Thr Thr Ala
595 600 605
Ala Asn Thr Ala Lys Ser Ser Ala Thr Gly Gly Ala Asp Ala Glu Ser
610 615 620
Ala Gln Ser Gly Asp Lys Asn Gly Ala Gly Lys Thr Gly Val His Ser
625 630 635 640
Leu Ala Ile Ser Met Val Phe Ala Ala Met Gly Leu Leu Leu Ile
645 650 655
<210> 189
<211> 545
<212> PRT
<213> Glomerella graminicola
<400> 189
Met Thr Leu Pro Val Ile Pro Tyr Thr Thr Gln Asp Lys Thr Asn Val
1 5 10 15
Ala Pro Arg Gly Leu Ser Leu Pro Ala Asp Glu Asp Ala Glu Thr Ser
20 25 30
Met Met Met Thr Leu Pro Val Thr Ser His Gln Arg Arg Lys Arg Arg
35 40 45
Thr Arg Ser Pro Arg Lys Val Leu Leu Asp Asp Ile Leu Asp Ser Asn
50 55 60
Leu Ala Pro Leu Pro Ala Ala Pro Val Val Lys Pro Pro Ile Ser Met
65 70 75 80
Arg Gly Leu Pro Val Val Asp Gly Ala Asp Ala Asn Gly Ala Phe Val
85 90 95
Thr Leu Pro Val Ile His Ser Thr Lys Arg Gly Val Phe Ser Arg Gln
100 105 110
Val Glu Ala Lys Leu Ala Asn Arg Ser Asp Val Ala Tyr Tyr Ala Gln
115 120 125
Leu Asn Ile Gly Thr Pro Pro Gln Ala Val Tyr Ala Gln Leu Asp Thr
130 135 140
Gly Ser Phe Glu Leu Trp Val Asn Pro Asp Cys Asn Val Leu Ala Ala
145 150 155 160
Ser Asp Gln Arg Phe Cys Glu Ala Val Gly Phe Tyr Asp Pro Ala Arg
165 170 175
Ser Thr Thr Ser Val Gln Met Gln Thr Thr Lys Thr Leu Arg Tyr Gly
180 185 190
Ile Gly Ala Ala Asn Ile Ser Tyr Val Arg Asp Ser Leu Ala Leu Ala
195 200 205
Gly Ser Ser Ser Thr Met Arg Gln Val Gln Phe Gly Val Ala Thr Ser
210 215 220
Ser Glu Asp Gln Phe Ala Gly Ile Leu Gly Ile Gly Ala Gly Glu Gly
225 230 235 240
Val Asn Thr Arg Tyr Lys Asn Leu Ile Asp Glu Leu Ala Ala Gln Gly
245 250 255
Val Thr Arg Thr Lys Ala Phe Ser Leu Gly Leu Gly Ser Lys Asp Glu
260 265 270
Gln Glu Gly Ala Ile Ile Phe Gly Gly Ile Asp Thr Ser Lys Phe Ser
275 280 285
Gly Pro Leu Ala Arg Leu Pro Ile Ile Pro Ala Asp Glu Ala Pro Asp
290 295 300
Gly Val Ala Arg Tyr Trp Val Asn Met Gln Ser Leu Ser Leu Thr Pro
305 310 315 320
Pro Ser Arg Arg Thr Arg Leu Tyr Ala Asn Ser Ser Met Pro Val Phe
325 330 335
Leu Asp Ser Gly Ala Thr Leu Thr Leu Leu Pro Glu Glu Leu Ala Asn
340 345 350
Met Ile Ala Ala Asp Phe Gly Ser Ser Gly Ile Asp Ala Asn Gly Phe
355 360 365
Tyr Pro Val Ser Cys Asn Leu Val Gly Leu Asn Gly Thr Val Asp Phe
370 375 380
Asp Phe Asp Gly Met Lys Ile Gln Val Pro Tyr Ser Glu Ile Ile Arg
385 390 395 400
Glu Leu Arg Thr Thr Pro Pro Thr Cys Tyr Leu Gly Ile Val Pro Asn
405 410 415
Ala Asp Phe Thr Leu Leu Gly Asp Thr Phe Leu Arg Ser Ala Tyr Ala
420 425 430
Val Phe Asp Leu Asp Ser Asn Val Ala Tyr Leu Ala Gln Tyr Thr Asn
435 440 445
Cys Gly Thr Arg Thr Met Pro Ile Asn Gln Pro Gln Asp Ile Ala Ala
450 455 460
Ile Arg Gly Leu Cys Pro Ser Pro Ala Gly Pro Gly Gly Ser Leu Pro
465 470 475 480
Ala Ala Asn Gly Thr Thr Asn Ser Pro Ser Ser Ser Ser Gly Ser Asn
485 490 495
Arg Val Pro Ser Thr Gly Ser Asn Val Pro Gly Ser Pro Thr Pro Glu
500 505 510
Thr Ser Gly Val Pro Ala Gly Thr Ala Ser Ala Ala Arg Ala Thr Gly
515 520 525
Arg Ser Met Leu Ala Thr Val Ser Ala Leu Leu Leu Ala Ala Met Phe
530 535 540
Trp
545
<210> 190
<211> 544
<212> PRT
<213> Metarhizium acridum
<400> 190
Met Val Ser Phe Leu Ile Thr Thr Ala Val Leu Ser Leu Ser Leu Ser
1 5 10 15
Val His Gly Trp Pro Gln Val Arg Asn Glu Ser Arg Phe Phe Gly Glu
20 25 30
Glu Ile Arg Ala Glu Asn Ile Val Ala Asp Ile Ser Ser Arg Glu Leu
35 40 45
Pro Phe Val Gly Glu Ala Leu Ser Val Gly Thr Leu Pro Val Pro Gln
50 55 60
Leu Ser Gln Arg Asp Asp Ala Val Gly Ser Leu Glu Ala Thr Glu Asp
65 70 75 80
Asp Gln Asp Ser Ser Ser Asn Asn Ser Phe Gln Glu Asp Ala Lys Arg
85 90 95
Gly Pro Pro Glu Gly Asp Ile Ser Pro Gly His Leu Thr Leu Pro Val
100 105 110
Leu His Val Glu Arg Arg Leu Asp Lys Arg Asp Val Glu Val Arg Leu
115 120 125
Glu Asn Arg Ser Asp Val Ala Tyr Tyr Val Gln Pro Asn Leu Gly Thr
130 135 140
Asn Pro Asp Pro Lys Lys Phe Lys Val Asn Ile Gly Asn Pro Val Gln
145 150 155 160
Lys Val Phe Ala Gln Leu Asp Thr Gly Ser Phe Glu Leu Trp Val Asn
165 170 175
Pro Asp Cys Thr Ser Leu Pro Ser Ser Ala Asp Lys Gln Phe Cys Gln
180 185 190
Ala Val Gly Gln Tyr Asn Pro Leu Arg Ser Ser Ser Ser Lys Gly Ser
195 200 205
Asn Leu Ser Thr Glu Leu Arg Tyr Gly Ile Gly Ser Ala Gln Ile Thr
210 215 220
Tyr Phe Leu Asp Asp Ile Ala Leu Val Asp Ser Arg Gln Met Lys Gln
225 230 235 240
Val Gln Phe Gly Val Ala Arg Ser Thr Lys Asp Gln Phe Ser Gly Ile
245 250 255
Leu Gly Ile Gly His Gly Val Gly Val Asn Thr Gly Tyr Arg Asn Phe
260 265 270
Val Asp Gln Leu Ala Asp Gln Gly Ile Thr Lys Thr Lys Ala Tyr Ser
275 280 285
Val Ala Leu Gly Ser Lys Ala Asp Arg Ala Gly Val Val Val Met Gly
290 295 300
Gly Val Asp Thr Ala Lys Phe Ala Gly Arg Leu Thr Pro Leu Pro Leu
305 310 315 320
Ile Pro Gly Asp Lys Ser Pro Asp Gly Val Thr Arg Phe Trp Val Asn
325 330 335
Leu Thr Ser Val Gln His Asn Ser Glu Ser Gly Arg Thr Ala Asn Leu
340 345 350
Thr Gly Ala Thr Met Pro Val Phe Met Asp Thr Gly Ala Thr Leu Thr
355 360 365
Leu Leu Pro Pro Arg Leu Ala Asn Asp Ile Ala Arg Arg Val Gly Ala
370 375 380
Arg Gly Leu Asp Arg Asn Gly Leu Tyr Met Val Asp Cys Gly Val Ala
385 390 395 400
Gly Lys Asn Gly Ser Leu Asp Phe Asn Phe Asp Gly Val Thr Ile Arg
405 410 415
Val Pro Tyr Ser Glu Met Val Arg Glu Ile Ala Thr Leu Pro Pro Ser
420 425 430
Cys Tyr Leu Gly Ile Met Pro Ser Thr Ser Phe Thr Leu Leu Gly Asp
435 440 445
Thr Phe Leu Arg Ser Ala Tyr Val Leu Phe Asp Ile Ser Asn Asn Met
450 455 460
Thr Tyr Met Ala Pro Tyr Ala Asn Cys Gly Ser Lys Thr Gln Ser Ile
465 470 475 480
Asp Ser Ser Thr Asp Leu Thr Ser Ile Val Gly Thr Cys Ser Pro Gln
485 490 495
Gln Val Asn Glu Leu Ser Lys Pro Val Gly Gly Gly Ser Ser Asp Gly
500 505 510
Asn Ser Gly Asn Lys Asp Asn Gly Ala Gly Leu Ala Arg Met Ser Trp
515 520 525
Ser Trp Phe Gly Val Leu Gly Ala Phe Ala Ile Gly Ala Leu Thr Leu
530 535 540
<210> 191
<211> 506
<212> PRT
<213> 太瑞斯梭孢壳霉
<400> 191
Met Ala Phe Ser Phe Leu Leu Leu Pro Thr Val Leu Leu Ile Pro Leu
1 5 10 15
Phe Cys Arg His Val Thr Ala Leu Gln Ala Thr Ala Leu Arg Gln Thr
20 25 30
Ala His Asp Pro Gly Cys Val His Leu Pro Val Ile His Ser Thr Asn
35 40 45
Val Asn Tyr Phe Ser Ser Lys Arg Ala Val Gln Leu Gln Leu Ala Asn
50 55 60
Arg Ser Asp Val Ala Tyr Tyr Ala Gln Leu Ser Ile Gly Thr Pro Pro
65 70 75 80
Gln Pro Val Phe Val Gln Leu Asp Thr Gly Ser Phe Glu Leu Trp Val
85 90 95
Asn Pro Asp Cys Thr Ser Ile Thr Gly Ala Asp Ala Ala Phe Cys Glu
100 105 110
Arg Thr Gly Gln Phe Asp Thr Lys Lys Ser Ser Thr Ala Ile Ser Leu
115 120 125
Gly Ala Thr Lys Thr Leu Gln Tyr Gly Ile Gly Ser Ala Asn Ile Thr
130 135 140
Tyr Phe Thr Asp Thr Ile Thr Leu Ala Gly Ser Ser Ala Ala Leu Gln
145 150 155 160
Ala Val Gln Phe Gly Val Ala Thr Ala Ser Gln Asp Ala Ser Ser Gly
165 170 175
Ile Leu Gly Ile Gly Tyr Gly Lys Gly Ile Ala Thr Asn Tyr Pro Asn
180 185 190
Phe Ile Asp Gln Leu Arg Ala Gln Asn Val Thr Arg Val Lys Ala Tyr
195 200 205
Thr Leu Ala Leu Gly Ser Lys Asp Val Glu Glu Gly Val Ile Val Phe
210 215 220
Gly Gly Val Asp Thr Ser Lys Phe Ala Gly Lys Leu Ala Lys Leu Pro
225 230 235 240
Ile Ile Pro Ala Ala Gln Ser Pro Asp Gly Val Pro Arg Phe Trp Val
245 250 255
Glu Met Gln Ser Val Ser Met Thr Pro Pro Asn Gly Val Ser His Val
260 265 270
Tyr Asn Ala Ser Ser Thr Pro Leu Phe Phe Asp Ser Gly Ser Thr Met
275 280 285
Thr Leu Leu Pro Pro Asp Leu Thr Asn Ala Ile Ala Arg Asp Phe Gly
290 295 300
Ala Gln Gly Pro Asp Ala Asn Gly Phe Phe Arg Ile Asp Cys Ala Leu
305 310 315 320
Thr Thr Leu Asn Gly Thr Val Asp Phe Ala Phe Asp Gly Val Thr Ile
325 330 335
Lys Val Pro Tyr Lys Glu Leu Ala Arg Glu Val Ala Ser Ser Pro Pro
340 345 350
Val Cys Phe Leu Gly Ile Met Ser Ser Asp Arg Phe Thr Leu Leu Gly
355 360 365
Asp Thr Phe Leu Arg Ser Ala Tyr Ala Val Phe Asp Leu Glu Thr Asn
370 375 380
Ala Ile Trp Leu Ser Gln Ala Glu Asn Cys Gly Ser Ser Pro Ala Ala
385 390 395 400
Leu Asn Asn Ile Gln Asp Leu Ala Ala Val Thr Gly Lys Cys Gly Val
405 410 415
Thr Glu Ala Ala Asp Lys Asp Thr Ser Ser Thr Ala Val Ser Ser Val
420 425 430
Ala Pro Glu Ser Ala Glu Ala Leu Pro Ala Pro Thr Ala Ser Thr Val
435 440 445
Gly Leu Ala Ala Thr Gly Thr Thr Ser Pro Gly Arg Thr Thr Pro Thr
450 455 460
Ala Pro Thr Ala Ser Thr Thr Pro Thr Thr Pro Ser Ser Ser Ala Gly
465 470 475 480
Ser Cys Phe Arg Gly Gln Ala Gly Ile Val Trp Val Val Gly Val Met
485 490 495
Met Leu Leu His Met Leu Lys Leu Val Leu
500 505
<210> 192
<211> 524
<212> PRT
<213> 鹅掌柄孢壳
<400> 192
Met Ala Phe Ser Leu Leu Leu Ser Ala Ser Val Leu Leu Leu Ser Ser
1 5 10 15
Thr Phe Ser Gly His Ala Val Ala Ser Ser Leu Pro Phe Thr Asn Thr
20 25 30
Thr Ile Pro Pro Thr Asn Val Ser Ile Pro His Thr His Ala Pro Ile
35 40 45
Lys Leu Ile Asn Ser Ser Val Ile Ala His Asp Asp Gly Cys Val His
50 55 60
Leu Pro Ile Val His Ser Thr Asn Ala Asn His Phe Ser Lys Lys Arg
65 70 75 80
Gly Val Gln Leu Gln Leu Ala Asn Arg Ser Asp Ile Ala Tyr Tyr Ala
85 90 95
Gln Leu Ser Ile Gly Thr Pro Pro Gln Pro Val Phe Val Gln Leu Asp
100 105 110
Thr Gly Ser Phe Glu Leu Trp Val Asn Pro Asp Cys Thr Thr Val Gln
115 120 125
Gly Ala Asp Ala Ala Phe Cys Gln Gln Val Gly Arg Tyr Asp Gly Asn
130 135 140
Thr Ser Ser Thr Ala Lys Ser Leu Gly Thr Thr Lys Thr Leu Lys Tyr
145 150 155 160
Gly Ile Gly Thr Ala Asn Ile Thr Tyr Phe Glu Asp Thr Ile Thr Leu
165 170 175
Ala Gly Thr Ala Thr Ala Leu Gln Ala Val Gln Phe Gly Val Ala Thr
180 185 190
Ala Thr Glu Asp Thr Phe Ser Gly Ile Leu Gly Ile Gly Tyr Gly Lys
195 200 205
Gly Ile Ala Thr Lys Tyr Lys Asn Phe Val Asp Gln Leu Val Ala Gln
210 215 220
Asn Ala Ile Arg Ala Lys Ala Tyr Thr Leu Ala Leu Gly Ser Lys Glu
225 230 235 240
Glu Lys Glu Gly Val Ile Val Phe Gly Gly Val Asp Pro Ser Lys Phe
245 250 255
Ala Gly Pro Leu Ala Lys Leu Pro Ile Ile Asp Ala Asn Asn Ser Pro
260 265 270
Asp Gln Val Pro Arg Phe Trp Val Asp Met Asn Ser Ile Ala Ile Thr
275 280 285
Pro Pro Asn Asn Asp Thr Lys Val Tyr Glu Gly Ser Lys Met Pro Val
290 295 300
Phe Leu Asp Ser Gly Ser Thr Met Thr Leu Leu Pro Pro Asp Leu Ala
305 310 315 320
Ala Thr Ile Ala Lys Asp Phe Gly Ala Asp Lys Glu Asp Asp Asn Gly
325 330 335
Phe Tyr Arg Ile Asp Cys Gly Leu Thr Ser Met Asn Gly Thr Leu Asp
340 345 350
Phe Ser Phe Asn Gly Leu Asn Ile Arg Val Pro Tyr Lys Glu Met Ile
355 360 365
Arg Glu Val Pro Ser Asn Pro Pro Ala Cys Phe Leu Gly Ile Thr Pro
370 375 380
Ser Lys Lys Phe Thr Leu Leu Gly Asp Thr Phe Leu Arg Ser Ala Tyr
385 390 395 400
Val Val Phe Asp Leu Glu Thr Asn Ser Ile Trp Met Thr Gln Ala Thr
405 410 415
Ser Cys Gly Ser Thr Pro Ala Ala Leu Gly Ser Val Asn Asp Leu Ser
420 425 430
Met Val Val Gly Ala Cys Gly Leu Thr Ala Glu Pro Ala Val Thr Val
435 440 445
Ser Ser Ala Gly Gly Thr Thr Ala Thr Pro Gly Val Gly Val Gly Gly
450 455 460
Gly Gly Thr Gly Ser Asp Thr Asn Gly Ala Ser Ala Thr Val Ser Thr
465 470 475 480
Val Val Val Pro Glu Ser Pro Pro Gly Ala Thr Ser Thr Ala Gly Ser
485 490 495
Pro Val Thr Ser Ser Ser Ser Arg Cys His Pro Met Ala Val Val Ala
500 505 510
Met Ala Phe Ala Leu Leu Ala Leu Ala Asn Met Gly
515 520
<210> 193
<211> 496
<212> PRT
<213> 嗜热毛壳菌
<400> 193
Met Ala Val Pro Phe Leu Ser Ser Cys Leu Val Leu Ser Thr Val Trp
1 5 10 15
Leu Ser Ser Leu Val Thr Ala Gln His Leu Pro His Glu Lys Gly Cys
20 25 30
Val His Leu Pro Ile Val His Ser Thr Asn Ala Arg Leu Phe Gly Lys
35 40 45
Arg Gly Val Gln Leu Gln Leu Ala Asn Arg Ser Asp Val Ala Tyr Tyr
50 55 60
Ala Gln Leu Ser Ile Gly Thr Pro Pro Gln Pro Val Phe Val Gln Leu
65 70 75 80
Asp Thr Gly Ser Phe Glu Leu Trp Val Asn Pro Asp Cys Thr Thr Val
85 90 95
Glu Gly Ala Asp Ala Val Phe Cys Glu Arg Val Gly Gln Tyr Asp Ala
100 105 110
Ser Lys Ser Ser Thr Ala Val Ser Leu Gly Thr Ser Arg Thr Leu Arg
115 120 125
Tyr Gly Ile Gly Thr Ala Asn Ile Thr Tyr Val Thr Asp Thr Ile Thr
130 135 140
Leu Ala Gly Thr Gly Thr Thr Leu Gln Gly Val Gln Phe Gly Val Ala
145 150 155 160
Thr Ala Thr Glu Asp Ala Phe Ser Gly Ile Leu Gly Ile Gly Tyr Gly
165 170 175
Lys Gly Ile Ala Thr Arg Tyr Leu Asn Phe Val Asp Gln Leu Lys Glu
180 185 190
Gln Asn Ala Thr Lys Val Lys Ala Phe Ser Leu Ser Leu Gly Ser Lys
195 200 205
Asp Ala Gln Glu Gly Val Val Val Phe Gly Gly Val Asp Thr Ser Lys
210 215 220
Phe Ala Gly His Leu Thr Gln Val Pro Ile Ile Pro Ala Ser Gln Ser
225 230 235 240
Pro Asp Gly Val Pro Arg Phe Trp Val Ser Met Arg Ser Leu Ala Met
245 250 255
Thr Pro Pro Ser Gly Ile Ser Lys His Tyr Ser Gly Ser Glu Met Pro
260 265 270
Val Phe Leu Asp Ser Gly Ser Thr Met Thr Leu Leu Pro Pro Asn Leu
275 280 285
Thr Glu Ala Ile Ala Arg Asp Phe Gly Ser Lys Ser Glu Asp Ala Tyr
290 295 300
Gly Phe His Gln Ile Asp Cys Ser Leu Thr Lys Leu Asn Gly Thr Leu
305 310 315 320
Asp Phe Tyr Phe Asp Gly Val Met Ile Lys Val Pro Tyr Lys Glu Leu
325 330 335
Thr Arg Glu Val Pro Thr Asn Pro Pro Ala Cys Phe Leu Gly Ile Met
340 345 350
Ala Ser Asp Gln Phe Thr Leu Leu Gly Asp Thr Phe Leu Arg Ser Ala
355 360 365
Tyr Ala Leu Phe Asp Leu Glu Thr Asp Ser Ile Trp Met Thr Gln Ala
370 375 380
Ala Asn Cys Gly Ser Thr Pro Ala Ala Leu Asn Ser Val Glu Asp Ile
385 390 395 400
Gln Arg Ile Thr Gly Leu Cys Asp Leu Ser Val Pro Ala Asp Asn Thr
405 410 415
Thr Ser Ser Thr Glu Ala Phe Thr Pro Met Thr Glu Val Asp Gln Val
420 425 430
Ser Met Thr Thr Val Thr Ser Val Thr Pro Ile His Thr Ser Thr Thr
435 440 445
Ala Thr Ser Pro Thr Glu Glu Val Ser Ile Gln Ser Ser Ala Thr Ser
450 455 460
Ile Ser Gly Ala Asn Lys Leu Gly Arg Gly Asn Met Glu Gly Ile Leu
465 470 475 480
Phe Trp Gly Pro Gly Val Val Ile Ala Leu His Val Leu Ile Gly Val
485 490 495
<210> 194
<211> 479
<212> PRT
<213> 大丽轮枝菌
<400> 194
Met Thr Leu Pro Val Pro Val Thr Gly Gly Arg Gly Asp Gln His Asp
1 5 10 15
Gly Glu Ala Ser Arg Arg Gln Leu Pro Val Asp Asp Asp Asp Asp Asp
20 25 30
Thr Gly Ala Phe Tyr Thr Leu Pro Ile Ile His Ser Thr Lys Arg Gly
35 40 45
Val Phe Ser Arg Gln Ile Glu Val Glu Leu Ala Asn Arg Ser Asp Val
50 55 60
Ala Tyr Tyr Ala Gln Leu Asn Ile Gly Thr Pro Pro Gln Pro Val Tyr
65 70 75 80
Ala Gln Leu Asp Thr Gly Ser Phe Glu Leu Trp Val Asn Pro Asp Cys
85 90 95
Thr Val Leu Arg Pro Ala Asp Gln Arg Phe Cys Gln Ala Val Gly Val
100 105 110
Tyr Asp Pro Ala Arg Ser Ser Ser Ser Ser Leu Leu Ser Ser Thr Ala
115 120 125
Ser Leu Arg Tyr Gly Ile Gly Thr Ala Asn Ile Ser Tyr Val Arg Asp
130 135 140
Asp Val Ser Phe Ala Gly Ser Pro Val Leu Ser His Val Arg Phe Gly
145 150 155 160
Met Ala Thr Ala Thr Thr Asp Gln Phe Ala Gly Ile Leu Gly Leu Gly
165 170 175
His Gly Lys Asn Tyr Thr Val Thr Tyr Pro Asn Met Met Asp Glu Leu
180 185 190
Val Ala Gln Gly Ala Ile Arg Thr Lys Ala Tyr Ser Leu Ala Leu Gly
195 200 205
Ser Lys Thr Glu Gln Gln Gly Leu Leu Val Leu Gly Gly Ile Asp Thr
210 215 220
Ala Lys Tyr Ser Gly His Leu Ala Ala Leu Pro Val Ile Pro Ala Gln
225 230 235 240
Gln Ala Pro Asp Arg Val Ala Arg Phe Trp Val Asn Leu Arg Ser Leu
245 250 255
Ala Leu Thr Pro Pro Ser Gly Val Thr Arg Ala Pro Tyr Val Ala Ala
260 265 270
Ala Gly Leu Pro Val Phe Leu Asp Ser Gly Ala Thr Met Thr Leu Leu
275 280 285
Pro Arg Ala Leu Ala Asp Ala Ile Ala Ala Asp Phe Gly Ala Pro Pro
290 295 300
Ser Ser Ser Pro Asp Asp Pro Glu Thr Ser His Thr Phe Tyr Glu Val
305 310 315 320
Asp Cys Ser Met Ala Asp Ala Pro Gly Thr Leu Asp Phe Ser Phe Gly
325 330 335
Arg Ser Leu Thr Ile Arg Val Pro Tyr Ala Glu Met Val Arg Arg Thr
340 345 350
Arg Ala Asp Pro Pro Arg Cys Val Leu Gly Phe Val Pro Asp Asp Asp
355 360 365
Phe Ala Leu Leu Gly Asp Thr Phe Leu Arg Ser Ala Tyr Ala Val Phe
370 375 380
Asp Leu Glu Ser Asp Val Val Tyr Met Ala Gln Tyr Arg Asn Cys Gly
385 390 395 400
Glu Arg Pro Met Ala Val Arg Arg Val Gln Asp Ile His Ala Ile Val
405 410 415
Gly Leu Cys Glu Ala Pro Ala Arg Pro Ser Gln Ala Val Ala Ala Pro
420 425 430
Asp Thr Ala Pro Thr Glu Ala Ala Thr Phe Ala Ser Gly Pro Val Pro
435 440 445
Gly Leu Val Asp Val Ser Ala Ala Ala Gly Leu Ser Leu Ser Arg Ala
450 455 460
Trp Val Trp Ser Ile Ala Phe Ala Met Val Leu Val His Val Ala
465 470 475
<210> 195
<211> 489
<212> PRT
<213> 嗜热毁丝霉
<400> 195
Met Ala Ile Pro Val Leu Phe Ser Ala Leu Val Leu Leu Val Ala Leu
1 5 10 15
Leu Cys Cys His Ala Thr Ala Ala Leu Gln Gln Leu Ala His Asp Val
20 25 30
Gly Cys Val His Leu Pro Val Val His Ser Thr Lys Val Asp Arg Phe
35 40 45
Ser Asp Lys Arg Gly Ile Gln Leu Gln Leu Ala Asn Arg Ser Asp Val
50 55 60
Ala Tyr Tyr Ala Gln Leu Ser Ile Gly Thr Pro Pro Gln Pro Val Phe
65 70 75 80
Val Gln Leu Asp Thr Gly Ser Phe Glu Leu Trp Val Asn Pro Asp Cys
85 90 95
Thr Thr Val Ser Gly Ser Asp Ala Val Phe Cys Glu Arg Ala Gly Arg
100 105 110
Tyr Asp Val Thr Lys Ser Ser Thr Ala Thr Ser Leu Gly Thr Asn Arg
115 120 125
Thr Leu Arg Tyr Gly Ile Gly Ala Ala Asn Ile Ser Tyr Phe Thr Asp
130 135 140
Thr Ile Ser Leu Ala Gly Ser Pro Met Met Leu Gln Asp Val Gln Phe
145 150 155 160
Gly Val Ala Thr Ala Ser Glu Asp Ala Phe Ser Gly Ile Leu Gly Ile
165 170 175
Gly Tyr Gly Lys Gly Ile Gly Thr Gly Tyr Pro Asn Phe Val Asp Gln
180 185 190
Leu Trp Glu Gln Asn Val Thr Arg Val Lys Ala Tyr Thr Leu Ala Leu
195 200 205
Gly Ser Lys Asp Ser Gln Glu Gly Val Ile Val Phe Gly Gly Val Asp
210 215 220
Thr Ser Lys Phe Ala Gly Lys Leu Ala Arg Leu Pro Val Ile Pro Pro
225 230 235 240
Ala Gln Ser Pro Asp Gly Val Pro Arg Phe Trp Val Glu Met Lys Ser
245 250 255
Leu Ser Ile Thr Arg Pro Ser Gly Leu Asn Thr Val Tyr Asp Gly Gly
260 265 270
Ala Met Pro Val Phe Leu Asp Ser Gly Ser Thr Met Thr Leu Leu Pro
275 280 285
Ala Asn Leu Thr Met Ala Val Ala Arg Asp Phe Gly Ala Gln Ala Pro
290 295 300
Asp Ala Asn Gly Phe Tyr Lys Ile Asp Cys Ala Leu Thr Ala Leu Asn
305 310 315 320
Gly Thr Leu Asp Phe Ala Phe Asp Gly Val Thr Val Arg Val Pro Tyr
325 330 335
Lys Glu Leu Thr Arg Glu Val Ala Ser Asn Pro Pro Ser Cys Phe Leu
340 345 350
Gly Ile Val Ala Ser Asp Arg Phe Thr Leu Leu Gly Asp Thr Phe Leu
355 360 365
Arg Ser Ala Tyr Thr Val Phe Asp Leu Glu Thr Asp Ser Ile Trp Met
370 375 380
Ala Pro Ala Val Asn Cys Gly Ser Ser Pro Ala Ala Leu Ser Asn Val
385 390 395 400
Gln Asp Leu Ser Ala Val Thr Gly Glu Cys Gly Val Arg Glu Ile Ala
405 410 415
Glu Ser Thr Ser Ser Thr Gln Val Pro Ser Thr Gly Val Asp Asp Thr
420 425 430
Glu Ala Gly Ala Val Pro Thr Ser Thr Thr Thr Val Val Ser Gln Pro
435 440 445
Ser Gly Thr Thr Thr Gln Met Gly Ala Arg Pro Thr Leu Asp Asn Ala
450 455 460
Ser Asn Pro Leu Gly Ala His Arg Leu Thr Trp Val Leu Val Ile Thr
465 470 475 480
Ala Ala Leu His Leu Phe Thr Gly Ile
485
<210> 196
<211> 518
<212> PRT
<213> 粗糙链孢霉
<400> 196
Met Ala Ala Phe Pro Phe Leu Ser Ala Ser Phe Val Leu Leu Gln Leu
1 5 10 15
Ala Leu Thr Cys Leu Ala Gln His Leu Asn Leu Thr Thr Gly Pro Leu
20 25 30
His Leu Thr Gly His Thr Pro Gly Asp Gly Cys Val His Leu Pro Ile
35 40 45
Ile His Ser Thr Asn Thr Asp His Phe Ala Arg Arg Gly Ile Gln Leu
50 55 60
Ala Leu Asn Asn Arg Ser Asp Val Ala Tyr Tyr Ala Gln Leu Glu Ile
65 70 75 80
Gly Thr Pro Pro Gln Thr Val Tyr Thr Gln Leu Asp Thr Gly Ser Phe
85 90 95
Glu Leu Trp Val Asn Pro Asp Cys Thr Thr Val Ser Pro Ser Asp Ser
100 105 110
Ser Phe Cys Asp His Ile Gly Phe Tyr Asn Ala Ser Leu Ser Ser Thr
115 120 125
Ser Lys Ser Leu Gly Thr Ser Lys Thr Leu Arg Tyr Gly Ile Gly Ala
130 135 140
Ala Asn Ile Ser Tyr Val Thr Asp Thr Ile Ser Leu Ser Gly Ser Ser
145 150 155 160
Thr Ser Leu Lys Asp Ile Gln Phe Gly Val Ala Thr Ser Ser Lys Asp
165 170 175
Ala Phe Ser Gly Ile Leu Gly Ile Gly Tyr Gly Gln Gly Leu Ala Thr
180 185 190
Lys Tyr Pro Asn Phe Ile Asp Gln Leu Tyr Ala Gln Lys Ile Thr Lys
195 200 205
Val Lys Ala Tyr Thr Leu Ala Leu Gly Ser Lys Thr Ala Gln Gln Gly
210 215 220
Ser Ile Val Phe Gly Gly Val Asp Thr Ser Lys Phe Ala Gly Pro Leu
225 230 235 240
Gly Arg Leu Pro Ile Ile Pro Ala Glu Asp Ser Pro Asp Gly Val Pro
245 250 255
Arg Phe Trp Val Gln Met Asn Gly Ile Ser Leu Thr Pro Pro Ser Gly
260 265 270
Gln Ser Met Gly Val Tyr Glu Gly Ser Lys Ile Pro Ala Phe Leu Asp
275 280 285
Ser Gly Ser Thr Met Thr Ile Leu Pro Pro Ala Leu Ala Asn Lys Ile
290 295 300
Ala Glu Asp Phe Gly Ser Pro Glu Met Asp Ala Asn Gly Phe Tyr Arg
305 310 315 320
Val Gly Cys Gly Tyr Val Glu Met Asn Gly Thr Met Asp Phe Glu Phe
325 330 335
Val Gly Ala Gly Gln Lys Val Thr Val Arg Val Pro Tyr Lys Glu Met
340 345 350
Ile Arg Glu Val Gly Gln Gly Glu Ser Lys Met Cys Phe Leu Gly Ile
355 360 365
Met Gly Ser Glu Ser Phe Thr Leu Leu Gly Asp Thr Phe Leu Arg Ser
370 375 380
Ala Tyr Ala Thr Ser Cys Gly Asn Thr Pro Ala Ala Leu Arg Asp Val
385 390 395 400
Thr Asp Leu Ser Arg Val Val Gly Asn Cys Gln Ile Gln Leu Gly Glu
405 410 415
Lys Glu Ala Val Val Asp Val Val Ser Glu Thr Ser Ile Ala Pro Pro
420 425 430
Thr Gly Ser Thr Gly Asp Thr Asp Gly Val Thr Gly Thr Gly Gly Asn
435 440 445
Gly Ser Gly Asn Gly Gly Thr Arg Thr Ala Trp Gly Phe Val Thr Thr
450 455 460
Thr Leu Ala Val Pro Met Ala Thr Gly Leu Ala Gly Val Gly Gly Ser
465 470 475 480
Gly Ser Gly Ser Met Ser Ala Thr Ala Leu Asp Ser Ser Gly Arg Ser
485 490 495
Met Ala Gly Asp Val Val Leu Ser Ala Ala Val Ala Val Gly Ala Ala
500 505 510
Val Leu Gly Ser Leu Leu
515
<210> 197
<211> 497
<212> PRT
<213> 稻瘟病菌
<400> 197
Met Ala Ala Thr Ser Ile Arg Gly Leu Leu Leu Leu Ala Val Ala Ala
1 5 10 15
Ala Ala Gly Pro Leu Glu Ala Ala Gln Glu Pro His Asp Pro Gly Cys
20 25 30
Leu His Met Pro Ile Met His Met Ser Lys Ala Glu Gln Tyr Gln Lys
35 40 45
Arg Ala Val Lys Val Pro Phe Asp Gln Arg Phe Asp Val Gly Tyr Tyr
50 55 60
Thr Lys Leu Ser Phe Gly Thr Pro Ala Gln Glu Ala Leu Val His Leu
65 70 75 80
Asp Thr Gly Ser Phe Glu Leu Trp Ile Asn Pro Thr Cys Asn Asn Leu
85 90 95
Asp Gly Ala Gly Glu Gln Gln Phe Cys Arg Ala Gly Gly Ser Tyr Asp
100 105 110
Pro Ala Gln Ser Thr Thr Val Gln Arg Leu Gly Thr Thr Asn Thr Leu
115 120 125
Arg Tyr Gly Ile Gly Ser Ala Asn Ile Ser Tyr Val Lys Asp Thr Val
130 135 140
Gly Ile Pro Gly Thr Asn Met Ser Val Arg Ser Ala Gln Phe Gly Val
145 150 155 160
Ala Thr Ser Ser Glu Thr Gln Asn Ala Gly Ile Leu Gly Ile Gly His
165 170 175
Gly Val Gly Gln Ala Ile Gly Tyr Arg Asn Phe Ile Asp Glu Leu Lys
180 185 190
Ala Gln Asn Val Thr Lys Thr Arg Ala Phe Ser Val Ala Leu Gly Gly
195 200 205
Lys Asp Glu Lys Gln Gly Thr Val Val Phe Gly Gly Val Asp Thr Ser
210 215 220
Lys Phe Ala Gly Gly Leu Ala Arg Leu Pro Ile Ile Pro Ala Ala Gln
225 230 235 240
Ser Pro Asp Gly Val Arg Arg Tyr Trp Val Asn Met Ser Ser Ile Ser
245 250 255
Leu His Pro Pro Ser Gly Arg Asn Lys Thr Tyr Pro Gly Thr Asn Met
260 265 270
Pro Val Phe Leu Asp Thr Gly Ser Thr Leu Thr Leu Leu Pro Glu Ala
275 280 285
Leu Val Lys Ser Val Ala Ser Asp Phe Gly Ser Met Gln Gln Asp Ala
290 295 300
Gly Gly Tyr Trp Pro Val Asp Cys Ser Leu Val Gly Ile Asn Gly Thr
305 310 315 320
Met Asp Phe Ala Phe Asp Gly Val Thr Ile Lys Val Pro Tyr Lys Glu
325 330 335
Leu Ile Arg Gln Phe Gly Asn Ser Cys Val Leu Gly Leu Ser Ser His
340 345 350
Ser Ser Phe Thr Leu Leu Gly Asp Thr Phe Met Arg Ser Ala Tyr Phe
355 360 365
Val Phe Asp Gln Asp Ser Asp Cys Val Trp Met Thr Gln Tyr Thr Asn
370 375 380
Cys Gly Ser Thr Pro Ala Ala Leu Ala Ser Val Ala Ser Leu Arg Thr
385 390 395 400
Leu Gln Gly Ala Cys Pro Leu Lys Gln Gly Ser Val Ser Ala Val Thr
405 410 415
Ala Gly Thr Ala Gly Ala Ala Ala Pro Ser Thr Thr Ala Ser Pro Lys
420 425 430
Ala Pro Gly Ala Gly Gly Gly Ser Gly Ser Asn Gly Gly Gly Gly Ala
435 440 445
Lys Gly Ser Pro Pro Pro Gly Ala Ser Ala Ser Ala Pro Asp Ser Lys
450 455 460
Leu Lys Asn Ala Gly Gly Arg Ala Met Gln Val Pro Ala Ile Ser Ser
465 470 475 480
Ala Val Val Thr Leu Val Leu Thr Thr Leu Ala Ala Leu His Leu Ala
485 490 495
Leu
<210> 198
<211> 434
<212> PRT
<213> 球毛壳霉
<400> 198
Met Pro Gln His Ser Asn Lys Pro Ser Thr Thr Thr Val Ser Leu Gly
1 5 10 15
Thr Pro Pro Gln Pro Val Phe Val Gln Leu Asp Thr Gly Ser Phe Glu
20 25 30
Leu Trp Val Asn Pro Asp Cys Thr Thr Val Ser Gly Gly Asp Ala Val
35 40 45
Phe Cys Gln Arg Ala Gly Arg Tyr Glu Ser Thr Ala Ser Ser Thr Ala
50 55 60
Thr Ser Leu Gly Thr Thr Arg Thr Leu Arg Tyr Gly Ile Gly Ala Ala
65 70 75 80
Asn Ile Ser Tyr Phe Thr Asp Thr Ile Ser Leu Ala Gly Ser Pro Met
85 90 95
Ser Leu Gln Asp Val Gln Phe Gly Val Ala Thr Ala Ser Val Asp Ala
100 105 110
Phe Ser Gly Ile Leu Gly Ile Gly Tyr Gly Lys Gly Ile Ala Thr Arg
115 120 125
Tyr Pro Asn Phe Val Asp Gln Leu Arg Glu Gln Asn Ala Thr Arg Val
130 135 140
Lys Ala Tyr Thr Leu Ala Leu Gly Ser Lys Asp Ala Gln Glu Gly Val
145 150 155 160
Ile Val Phe Gly Gly Val Asp Thr Ser Lys Phe Ala Gly Lys Leu Ala
165 170 175
Lys Leu Pro Ile Leu Pro Ala Ala Gln Ala Pro Asp Gly Val Pro Arg
180 185 190
Phe Trp Val Asp Met Gln Ala Leu Ser Ile Thr Pro Pro Asn Gly Val
195 200 205
Thr Thr Val Tyr Glu Gly Ser Ser Met Pro Val Phe Leu Asp Ser Gly
210 215 220
Ser Thr Met Thr Leu Leu Pro Ala Asn Leu Thr Ala Ala Val Ala Arg
225 230 235 240
Asp Phe Gly Ala Glu Ser Pro Asp Ser Asn Gly Phe Tyr Met Ile Asp
245 250 255
Cys Ala Leu Thr Ala Leu Asn Gly Thr Leu Asp Phe Val Phe Asp Gly
260 265 270
Val Thr Val Lys Val Pro Tyr Lys Glu Leu Thr Arg Glu Val Ala Ser
275 280 285
Thr Pro Pro Ser Cys Phe Leu Gly Ile Met Ala Ser Asp Arg Phe Thr
290 295 300
Leu Leu Gly Asp Thr Phe Leu Arg Ser Ala Tyr Thr Val Phe Asp Leu
305 310 315 320
Glu Thr Asp Ser Ile Trp Met Thr Gln Ala Thr Asn Cys Gly Ser Ser
325 330 335
Pro Ala Ala Leu Ser Asn Ile Gln Asp Leu Ser Val Val Val Gly Glu
340 345 350
Cys Gly Ala Gly Ala Ser Glu Asp Ile Asp Thr Thr Ser Ser Thr Gln
355 360 365
Phe Pro Ser Thr Ala Val Glu Asp Phe Glu Ser Gly Ala Ile Pro Thr
370 375 380
Ser Thr Gly Ser Phe Gly Pro Gln Val Ser Glu Val Ala Pro Pro Asp
385 390 395 400
Gln Ala Arg Pro Leu Pro Glu Asn Ser Gly Pro Ser Leu Arg Ala Ala
405 410 415
Gly Phe Ile Trp Asn Leu Gly Met Ala Gly Ala Val His Leu Leu Ala
420 425 430
Gly Leu
<210> 199
<211> 467
<212> PRT
<213> 稻瘟病菌
<400> 199
Met Lys Asn Thr Ala Leu Leu Val Ala Thr Ala Ala Ala Ser Val Ala
1 5 10 15
Ala Val Gly Ile Ala Glu Ile Pro Arg Glu Gln Val Ser Thr Ser Ser
20 25 30
Gln Pro Ser Thr Ser Asp Pro Gly Phe Val Ser Phe Pro Ile Tyr His
35 40 45
Arg Ser Val Ser Lys Pro Ile Leu Lys Arg Asn Thr Asp Val Thr Ile
50 55 60
Tyr Asn Ile Ser Ser Val Ser Tyr Leu Ile Gln Leu Ser Ile Gly Thr
65 70 75 80
Pro Gly Gln Gln Val Lys Val Ala Ile Asp Thr Gly Ser Asp Glu Leu
85 90 95
Trp Val Asn Pro Asn Cys Thr Ala Val Lys Ser Gln Asp Gln Arg Ala
100 105 110
Glu Cys Ile Ala Asp Gly Gln Tyr Asn Pro Gly Gln Ser Ser Thr Ser
115 120 125
Gln Val Ser Thr Thr Ser Asn Asn Ile Pro Tyr Gly Lys Gly Asp Val
130 135 140
Asn Ile Thr Tyr Tyr Lys Asp Ser Ile Ser Met Ser Asn Ser Ser Ile
145 150 155 160
Ser Leu Thr Asn Val Ile Phe Gly Val Ala Thr Ser Ser Lys Asp Leu
165 170 175
Asn Glu Gly Ile Met Gly Leu Gly Tyr Gly Asp Gly Val Asn Leu Arg
180 185 190
Tyr Asn Asn Met Val Asp Ser Leu Phe Leu Gln Asn Val Thr Lys Ser
195 200 205
Arg Ala Phe Ser Val Ala Leu Gly Ser Ala Glu Ala Ser Asn Ser Gly
210 215 220
Val Ile Ser Phe Gly Gly Val Asp Thr Lys Lys Phe Ser Gly Lys Leu
225 230 235 240
Gly Gln Met Pro Ile Leu Gly Pro Gln Gly Lys Glu Glu Ile Arg Arg
245 250 255
Tyr Trp Val Pro Met Asp Ser Ile Ala Met Ser Lys Asn Gly Gly Asn
260 265 270
Lys Thr Tyr Ser Gly Gly Asn Ile Pro Ile Val Ile Asp Ser Gly Ser
275 280 285
Thr Leu Ser Tyr Leu Pro Ser Ser Val Val Thr Ala Met Ala Met Asp
290 295 300
Ala Asn Ala Leu Trp Asn Ser Glu Gly Gly Val Tyr Phe Val Lys Cys
305 310 315 320
Asp Lys Met Pro Ala Asp Ala Ser Phe Asp Phe Ser Phe Asn Asn Gly
325 330 335
Ser Phe Arg Ile Ser Val Pro Trp Arg Asn Phe Phe Trp Asn Thr Gly
340 345 350
Gly Asn Thr Cys Val Leu Gly Ala Val Pro Ile Lys Ser Gly Ser Val
355 360 365
Thr Ala Leu Leu Gly Asp Ser Phe Met Arg Ser Val Tyr Thr Val Phe
370 375 380
Asp Gln Thr Ser Asn Met Val Phe Met Ala Pro Tyr Ala Asn Cys Gly
385 390 395 400
Ser Asn Glu Gln Met Ile Pro Ala Ser Pro Ser Gly Ala Ala Ala Asn
405 410 415
Phe Thr Gly Glu Cys Ser Pro Gly Gln Gly Leu Pro Asp Pro Thr Pro
420 425 430
Gln Lys Asn Ala Ala Gly Lys Ala Ser Gly Glu Pro Ser Lys Phe Glu
435 440 445
Phe Trp Ala Cys Ala Ala Val Leu Val Val Gly His Leu Val Phe Gly
450 455 460
Met Leu Leu
465
<210> 200
<211> 387
<212> PRT
<213> 里氏木霉
<400> 200
Met Gly Leu Val Thr Asn Pro Phe Ala Lys Asn Ile Ile Pro Asn Arg
1 5 10 15
Tyr Ile Val Val Tyr Asn Asn Ser Phe Gly Glu Glu Ala Ile Ser Ala
20 25 30
Lys Gln Ala Gln Phe Ala Ala Lys Ile Ala Lys Arg Asn Leu Gly Lys
35 40 45
Arg Gly Leu Phe Gly Asn Glu Leu Ser Thr Ala Ile His Ser Phe Ser
50 55 60
Met His Thr Trp Arg Ala Met Ala Leu Asp Ala Asp Asp Ile Met Ile
65 70 75 80
Lys Asp Ile Phe Asp Ala Glu Glu Val Ala Tyr Ile Glu Ala Asp Thr
85 90 95
Lys Val Gln His Ala Ala Leu Val Ala Gln Thr Asn Ala Ala Pro Gly
100 105 110
Leu Ile Arg Leu Ser Asn Lys Ala Val Gly Gly Gln Asn Tyr Ile Phe
115 120 125
Asp Asn Ser Ala Gly Ser Asn Ile Thr Ala Tyr Val Val Asp Thr Gly
130 135 140
Ile Arg Ile Thr His Ser Glu Phe Glu Gly Arg Ala Thr Phe Gly Ala
145 150 155 160
Asn Phe Val Asn Asp Asp Thr Asp Glu Asn Gly His Gly Ser His Val
165 170 175
Ala Gly Thr Ile Gly Gly Ala Thr Phe Gly Val Ala Lys Asn Val Glu
180 185 190
Leu Val Ala Val Lys Val Leu Asp Ala Asp Gly Ser Gly Ser Asn Ser
195 200 205
Gly Val Leu Asn Gly Met Gln Phe Val Val Asn Asp Val Gln Ala Lys
210 215 220
Lys Arg Ser Gly Lys Ala Val Met Asn Met Ser Leu Gly Gly Ser Phe
225 230 235 240
Ser Thr Ala Val Asn Asn Ala Ile Thr Ala Leu Thr Asn Ala Gly Ile
245 250 255
Val Pro Val Val Ala Ala Gly Asn Glu Asn Gln Asp Thr Ala Asn Thr
260 265 270
Ser Pro Gly Ser Ala Pro Gln Ala Ile Thr Val Gly Ala Ile Asp Ala
275 280 285
Thr Thr Asp Ile Arg Ala Gly Phe Ser Asn Phe Gly Thr Gly Val Asp
290 295 300
Ile Tyr Ala Pro Gly Val Asp Val Leu Ser Val Gly Ile Lys Ser Asp
305 310 315 320
Ile Asp Thr Ala Val Leu Ser Gly Thr Ser Met Ala Ser Pro His Val
325 330 335
Ala Gly Leu Ala Ala Tyr Leu Met Ala Leu Glu Gly Val Ser Asn Val
340 345 350
Asp Asp Val Ser Asn Leu Ile Lys Asn Leu Ala Ala Lys Thr Gly Ala
355 360 365
Ala Val Lys Gln Asn Ile Ala Gly Thr Thr Ser Leu Ile Ala Asn Asn
370 375 380
Gly Asn Phe
385
<210> 201
<211> 445
<212> PRT
<213> 深绿木霉
<400> 201
Met Ala Pro Phe Asn Thr Met Leu Gly Tyr Leu Leu Leu Ala Val Ala
1 5 10 15
Pro Leu Ala Ala Ala Leu Pro Phe His Gly His Gly His Asn Thr Asn
20 25 30
Asn His Asn Leu Asn Leu Asn Phe Asn Leu Thr Glu Thr Ile Asn Glu
35 40 45
Ile Asn Glu Asn Leu Ala Gly Leu Val Gly Tyr Ile Thr Asn Pro His
50 55 60
Ala Lys Asn Val Ile Ala Asn Arg Tyr Ile Val Val Tyr Asn Asn Thr
65 70 75 80
Phe Gly Ser Glu Ala Ile Ala Ala Lys Gln Ala Glu Phe Ala Ala Thr
85 90 95
Ile Gln Lys Arg Asn Leu Gly Lys Arg Ser Leu Gly Gly Asn Leu Leu
100 105 110
Ser Thr Glu Ile His Ser Phe Gln Met His Asn Trp Arg Ala Met Ala
115 120 125
Leu Asp Ala Asp Asp Glu Met Val Lys Ser Ile Phe Ser Ala Gln Glu
130 135 140
Val Ala Tyr Ile Glu Ala Asp Ala Val Val Gln Thr Lys Gly Ala Leu
145 150 155 160
Val Ala Gln Thr Asn Ala Thr Pro Gly Leu Ile Arg Leu Ser Asn Arg
165 170 175
Asn Ala Gly Gly Lys Asn Tyr Ile Phe Asp Asn Thr Ala Gly Ala Gly
180 185 190
Ile Thr Ala Tyr Val Val Asp Thr Gly Ile Arg Ile Thr His Ser Glu
195 200 205
Phe Glu Gly Arg Ala Ser Phe Gly Ala Asn Phe Val Asn Ser Asn Asn
210 215 220
Thr Asp Glu Asn Gly His Gly Ser His Val Ser Gly Thr Ile Gly Gly
225 230 235 240
Ala Thr Phe Gly Val Ala Lys Asn Val Lys Leu Val Ala Val Lys Val
245 250 255
Leu Asp Ala Thr Gly Ala Gly Ser Asn Ser Gly Val Leu Asn Gly Met
260 265 270
Gln Phe Val Val Asn Asp Val Gln Ala Lys Gly Leu Ser Gly Lys Ala
275 280 285
Val Met Asn Met Ser Leu Gly Gly Ser Leu Ser Ala Ala Val Asn Asn
290 295 300
Ala Ile Ala Ala Ile Ala Asn Ala Gly Val Val Pro Val Val Ala Ala
305 310 315 320
Gly Asn Glu Asn Gln Asp Thr Ala Asn Thr Ser Pro Gly Ser Ala Pro
325 330 335
Gln Ala Ile Thr Val Gly Ala Ile Asp Ala Thr Asn Asp Val Arg Ala
340 345 350
Ser Phe Ser Asn Phe Gly Pro Asp Val Asp Ile Tyr Ala Gln Gly Val
355 360 365
Asn Val Leu Ser Val Gly Ile Lys Ser Asp Thr Asp Thr Ala Val Leu
370 375 380
Ser Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Leu Ala Ala Tyr
385 390 395 400
Leu Met Ala Leu Lys Gly Leu Thr Asn Val Asp Asp Val Thr Thr Leu
405 410 415
Ile Lys Asn Leu Ala Thr Ala Thr Gly Ala Ser Val Gln Gln Asn Val
420 425 430
Ala Gly Thr Thr Asn Leu Ile Ala Asn Asn Gly Glu Leu
435 440 445
<210> 202
<211> 437
<212> PRT
<213> 绿木霉
<400> 202
Met Ala Pro Phe Asn Thr Met Leu Ala Tyr Leu Leu Leu Val Val Ala
1 5 10 15
Pro Leu Ala Ala Ala Val Pro Phe His Asn Arg His Ser Leu Gly Gly
20 25 30
Pro Gln Thr His Ala His Asn Thr Val Asn Asn Ile Asn Leu Ala His
35 40 45
Leu Met Asn Ile Val Val Asn Pro Thr Ala Lys Asn Ile Ile Pro Gly
50 55 60
Arg Tyr Ile Val Val Tyr Asn Asn Thr Phe Gly Asp Glu Ala Ile Ser
65 70 75 80
Ala Lys Gln Ala Gln Phe Ala Ser Thr Ile Ala Lys Arg Asn Leu Gly
85 90 95
Lys Arg Asn Leu Val Gly Asn Leu Leu Ser Thr Glu Ile His Ser Phe
100 105 110
Lys Met His Thr Trp Arg Ala Met Ala Leu Asp Ala Asp Asp Asp Met
115 120 125
Ile Lys Asn Ile Phe Asp Ala Glu Glu Val Ala Tyr Ile Glu Ala Asp
130 135 140
Thr Lys Val Gln Asn Thr Ala Leu Val Ala Gln Thr Asn Ala Thr Pro
145 150 155 160
Gly Leu Ile Arg Ile Ser Asn Arg Asn Val Gly Ala Ala Asn Tyr Ile
165 170 175
Phe Asp Thr Ser Ala Gly Thr Gly Ile Thr Ala Tyr Val Val Asp Thr
180 185 190
Gly Ile Arg Val Thr His Thr Glu Phe Glu Gly Arg Ala Thr Phe Gly
195 200 205
Ala Asn Phe Val Asn Thr Asn Asn Thr Asp Glu Asn Gly His Gly Ser
210 215 220
His Val Ala Gly Thr Ile Gly Gly Ala Thr Phe Gly Val Ala Lys Asn
225 230 235 240
Val Lys Leu Val Ala Val Lys Val Leu Asp Ala Ser Gly Ser Gly Ser
245 250 255
Asn Ser Gly Val Leu Asn Gly Met Gln Phe Val Val Asn Asp Val Gln
260 265 270
Ala Lys Lys Leu Gly Gly Lys Ala Val Met Asn Met Ser Leu Gly Gly
275 280 285
Ser Phe Ser Thr Ala Val Asn Asn Ala Ile Thr Ala Leu Ala Asn Ala
290 295 300
Gly Ile Val Pro Val Val Ala Ala Gly Asn Glu Asn Gln Asp Thr Ala
305 310 315 320
Asn Thr Ser Pro Gly Ser Ala Pro Gln Ala Ile Thr Val Gly Ala Ile
325 330 335
Asp Ala Thr Asn Asp Val Arg Ala Ser Phe Ser Asn Phe Gly Ala Gly
340 345 350
Val Asp Ile Tyr Ala Pro Gly Val Asp Val Leu Ser Val Gly Ile Lys
355 360 365
Ser Asp Ile Asp Thr Ala Ile Leu Ser Gly Thr Ser Met Ala Thr Pro
370 375 380
His Val Ala Gly Leu Ala Ala Tyr Leu Met Ala Leu Gln Gly Val Ser
385 390 395 400
Asn Val Asn Asp Val Thr Asn Leu Ile Lys Asn Leu Ala Ala Lys Thr
405 410 415
Gly Ala Asp Val Gln Gln Asn Val Ala Gly Thr Thr Asn Leu Ile Ala
420 425 430
Asn Asn Gly Asn Leu
435
<210> 203
<211> 433
<212> PRT
<213> 有性哈茨木霉
<400> 203
Met Ala Pro Phe Asn Thr Met Leu Ala Tyr Leu Leu Met Val Val Ala
1 5 10 15
Pro Leu Ala Ala Ala Met Pro Phe His Asn Arg His Ser Leu Ser Pro
20 25 30
Gln Thr His Gln His Ser Ala Leu Asn Met Thr His Leu Met Ser Val
35 40 45
Val Ile Asn Pro His Ala Lys Asn Ile Ile Pro Gly Arg Tyr Ile Val
50 55 60
Val Tyr Asn Asn Thr Phe Gly Asp Ala Ala Ile Ser Ala Lys Gln Ala
65 70 75 80
Gln Phe Ala Thr Ala Ile Ala Lys Arg Asn Leu Gly Lys Arg Asp Gly
85 90 95
Leu Gly Asn Leu Leu Ser Thr Glu Ile His Ser Phe Gln Leu His Thr
100 105 110
Trp Arg Ala Met Ala Leu Asp Ala Asp Asn Asp Met Ile Lys Ser Ile
115 120 125
Phe Asp Ala Glu Glu Val Ala Tyr Ile Glu Ala Asp Thr Lys Val Gln
130 135 140
Asn Thr Ala Leu Val Ala Gln Thr Asn Ala Thr Pro Gly Leu Ile Arg
145 150 155 160
Leu Ser Asn Lys Asp Val Gly Gly Gln Asn Tyr Ile Phe Asp Asn Ser
165 170 175
Ala Ala Ser Gly Met Thr Ala Tyr Val Val Asp Thr Gly Ile Arg Val
180 185 190
Thr His Thr Glu Phe Glu Gly Arg Ala Thr Phe Gly Ala Asn Phe Val
195 200 205
Asn Thr Asn Asp Thr Asp Glu Asn Gly His Gly Ser His Val Ala Gly
210 215 220
Thr Ile Gly Gly Ala Thr Phe Gly Val Ala Lys Asn Ile Lys Leu Val
225 230 235 240
Ala Val Lys Val Leu Asp Ala Ser Gly Ser Gly Ser Asn Ser Gly Val
245 250 255
Leu Asn Gly Met Gln Phe Val Ile Asn Asp Val Gln Thr Lys Lys Leu
260 265 270
Ala Gly Lys Ala Val Met Asn Met Ser Leu Gly Gly Ser Phe Ser Thr
275 280 285
Ala Val Asn Asn Ala Ile Thr Ala Leu Ala Asn Ala Gly Ile Val Pro
290 295 300
Val Val Ala Ala Gly Asn Glu Asn Gln Asp Thr Ala Asn Thr Ser Pro
305 310 315 320
Gly Ser Ala Pro Gln Ala Ile Thr Val Gly Ala Ile Asp Ala Thr Asn
325 330 335
Asp Val Arg Ala Ser Phe Ser Asn Phe Gly Ala Gly Val Asp Ile Tyr
340 345 350
Ala Pro Gly Val Asn Val Leu Ser Val Gly Ile Lys Ser Asp Ile Asp
355 360 365
Thr Ala Thr Leu Ser Gly Thr Ser Met Ala Thr Pro His Val Ala Gly
370 375 380
Leu Ala Ala Tyr Leu Met Ala Leu Glu Gly Val Thr Asn Val Asp Asp
385 390 395 400
Val Ser Asn Leu Ile Lys Asn Leu Ala Thr Lys Thr Gly Ala Thr Val
405 410 415
Gln Gln Asn Val Ala Gly Thr Thr Asp Leu Ile Ala Asn Asn Ala Asn
420 425 430
Leu
<210> 204
<211> 442
<212> PRT
<213> 尖孢镰刀菌
<400> 204
Met Ala Leu Leu Arg Thr Phe Leu Ala Ser Leu Leu Val Ala Ala Pro
1 5 10 15
Phe Ala Ala Ala Ala Pro Ile Asp Val Ala Glu Thr Leu Gly Asp Met
20 25 30
Ala Asp Ser Ile Gly Gly Gly Leu Gly Gly Glu Ala Asp Lys Lys Met
35 40 45
Gly Ile Ser Ala Phe Leu Arg Pro Met Leu Lys Asn Pro Asp Ala Leu
50 55 60
Asn Val Ile Pro Asn Arg Tyr Ile Val Val Tyr Asn Asp Thr Phe Asp
65 70 75 80
Asp Asp Thr Ile Ser Ala Lys Glu Ala Ser Phe Ala Ala Ala Ile Lys
85 90 95
Lys Arg Asn Leu Asn Lys Arg Ser Ser Ile Gly Lys Ala Met Ser Thr
100 105 110
Ser Ile Gln Ser Phe Arg Met Asn Lys Trp Arg Ala Met Ser Leu Asp
115 120 125
Ala Asp Asp Leu Met Val Gln Asp Leu Trp Asn Ser Asp Glu Val Ala
130 135 140
Tyr Ile Glu Ala Asp Thr Lys Val Gln Leu Asn Ala Ala Ile Ala Gln
145 150 155 160
Val Asn Ala Pro Pro Gly Leu Asp Arg Leu Ser His Ala Lys Val Asn
165 170 175
Gln Asp Thr Tyr Val Phe Asp Asp Ser Ala Gly Glu Gly Ile Thr Ala
180 185 190
Tyr Val Val Asp Thr Gly Ile Lys Ile Asp His Ser Glu Phe Glu Gly
195 200 205
Arg Ala Thr Phe Gly Ala Asn Phe Ile Asn Asn Val Asp Asp Asp Glu
210 215 220
Asn Gly His Gly Ser His Val Ala Gly Thr Ile Gly Gly Ala Thr Phe
225 230 235 240
Gly Val Ala Lys Lys Val Asp Leu Val Ala Val Lys Val Leu Asp Ala
245 250 255
Ser Gly Gly Gly Ser Asn Ser Gly Val Leu Gln Gly Met Gln Phe Val
260 265 270
Ile Asp Asp Ala Lys Lys Lys Asn Arg Val Gly Lys Ala Val Met Asn
275 280 285
Met Ser Leu Gly Gly Asp Phe Ser Gln Ala Ile Asn Arg Ala Ile Glu
290 295 300
Ala Leu Phe Lys Ala Gly Ile Val Pro Val Val Ala Ala Gly Asn Glu
305 310 315 320
Asn Arg Glu Thr Ala Leu Thr Ser Pro Gly Ser Ala Pro Asn Ala Ile
325 330 335
Thr Val Gly Ala Ile Asp Ala Thr Thr Asp Glu Arg Ala Asp Phe Ser
340 345 350
Asn Phe Gly Pro Glu Val Asp Val Tyr Ala Pro Gly Val Asn Val Leu
355 360 365
Ser Val Gly Ile Lys Ser Asn Thr Asp Thr Ala Thr Leu Ser Gly Thr
370 375 380
Ser Met Ala Ser Pro His Val Ala Gly Leu Ala Ala Tyr Leu Met Gly
385 390 395 400
Phe Gln Gln Leu Asp Gly Pro Ala Gln Val Ala Ser Leu Ile Lys Ser
405 410 415
Leu Ala Gly Gln Thr Gly Ala Lys Val Gln Asn Asn Val Gln Gly Thr
420 425 430
Thr Asp Ser Ile Ala Asn Asn Gly Asn Gln
435 440
<210> 205
<211> 446
<212> PRT
<213> 玉米赤霉
<400> 205
Met Ala Phe Leu Lys Thr Phe Leu Ala Ser Leu Leu Ile Ala Ala Pro
1 5 10 15
Leu Ala Thr Ala Ala Pro Val Asp Val Ala Gln Thr Ile Gly Asn Met
20 25 30
Ala Glu Ala Val Gly Ser Asp Ile Gly Gly Gly Leu Gly Glu Glu Leu
35 40 45
Asp Lys Lys Met Gly Ile Ser Ala Phe Leu Arg Pro Met Leu Ser Asn
50 55 60
Pro Asp Ser Leu Asn Ile Ile Pro Asn Arg Tyr Ile Val Val Tyr Asn
65 70 75 80
Asp Thr Phe Asp Asp Asp Ala Ile Ala Ala Lys Glu Phe Ser Ile Ala
85 90 95
Ser Ala Ile Lys Lys Arg Asn Leu Asn Lys Arg Ser Ser Ile Gly Gln
100 105 110
Ala Met Ser Thr Ser Ile Lys Ser Phe Arg Met Asn Thr Trp Arg Ala
115 120 125
Met Ser Leu Asp Ala Asp Asp Met Met Val Gln Asp Leu Phe Lys Ser
130 135 140
Asp Glu Val Ala Tyr Ile Glu Ala Asp Thr Arg Val Gln Leu Asn Ala
145 150 155 160
Ala Ile Ala Gln Val Asn Ala Pro Pro Gly Leu Asn Arg Leu Ser His
165 170 175
Ala Gln Ala Asp Gln Glu Asn Tyr Ile Phe Asp Asp Ser Ala Gly Glu
180 185 190
Gly Ile Thr Ala Tyr Val Val Asp Thr Gly Ile Lys Val Asp His Ser
195 200 205
Glu Phe Glu Gly Arg Ala Thr Phe Gly Gly Asn Phe Ile Asp Asn Val
210 215 220
Asp Asp Asp Glu Asn Gly His Gly Ser His Val Ala Gly Thr Ile Gly
225 230 235 240
Gly Ala Thr Phe Gly Val Ala Lys Lys Val Asp Leu Val Ala Val Lys
245 250 255
Val Leu Asp Ala Ser Gly Gly Gly Ser Asn Ser Gly Val Leu Gln Gly
260 265 270
Met Gln Phe Val Val Asp Asp Val Lys Lys Asn Asn Arg Ala Gly Lys
275 280 285
Ala Val Met Asn Met Ser Leu Gly Gly Asp Lys Ser Glu Ala Ile Asn
290 295 300
Arg Ala Ile Glu Ala Leu Phe Lys Ala Gly Val Val Pro Val Val Ala
305 310 315 320
Ala Gly Asn Glu Asn Arg Glu Thr Ala Leu Thr Ser Pro Gly Ser Ala
325 330 335
Pro Asn Ala Ile Thr Val Gly Ala Ile Asp Ala Thr Ser Asp Gln Arg
340 345 350
Ala Asp Phe Ser Asn Phe Gly Pro Glu Val Asp Ile Tyr Ala Pro Gly
355 360 365
Val Asp Val Leu Ser Val Gly Ile Lys Ser Asn Thr Asp Thr Ala Thr
370 375 380
Leu Ser Gly Thr Ser Met Ala Ser Pro His Val Ala Gly Leu Ala Ala
385 390 395 400
Tyr Leu Met Gly Phe Gln Lys Leu Asp Gly Pro Ala Gln Val Ala Ser
405 410 415
Leu Ile Lys Ser Leu Ala Ala Glu Ser Gly Ala Lys Val Arg Asn Asn
420 425 430
Val Arg Gly Thr Thr Asp Gly Ile Ala Asn Asn Gly Asn Gln
435 440 445
<210> 206
<211> 423
<212> PRT
<213> Glomerella graminicola
<400> 206
Met Val Arg Pro Ile Ala Ile Leu Ala Ala Val Leu Pro Ala Val Leu
1 5 10 15
Ala Ala Pro Phe Gly Leu Asn Glu Asn Lys Ala Ala Asn Asn Thr Asp
20 25 30
Thr Phe Met Gly Val Pro Ile Ser Asn Leu Asn Ala Leu Asp Leu Ile
35 40 45
Pro Asn Lys Tyr Ile Val Val Tyr Asn Asn Ser Phe Asp Asp Asp Ala
50 55 60
Ile Thr Ser Lys Met Ser Ser Phe Ala Ala Ala Ile Lys Lys Arg Asn
65 70 75 80
Ile Gly Lys Arg Ser Leu Asp Gly Arg Ser Leu Ser Thr Ser Thr Arg
85 90 95
Asn Phe Lys Met Asn Asn Trp Arg Ala Thr Val Met Glu Ala Asp Asp
100 105 110
Asn Met Ile Met Asn Val Met Ser Ala Glu Glu Val Ala Tyr Val Glu
115 120 125
Gln Asp Ala Lys Val Lys Ile Ser Ala Thr Val Ser Gln Thr Asn Ala
130 135 140
Pro Pro Gly Leu Val Arg Leu Ser His Ala Ala Ala Gly Glu Thr Gly
145 150 155 160
Tyr Val Phe Asp Gln Val Ser Gly Asp Gly Ile Thr Ala Tyr Val Val
165 170 175
Asp Thr Gly Ile Leu Thr Thr His Ser Glu Phe Gln Gly Arg Ala Thr
180 185 190
Phe Gly Ala Asn Phe Ile Asn Asn Val Asp Thr Asp Glu Asn Gly His
195 200 205
Gly Ser His Val Ala Gly Thr Ile Gly Gly Gln Thr Phe Gly Val Ala
210 215 220
Lys Asn Val Lys Leu Val Ala Val Lys Val Leu Asp Gly Asp Gly Ala
225 230 235 240
Gly Ser Asn Ser Gly Val Leu Asp Gly Met Gln Phe Val Ile Asp Asp
245 250 255
Val Lys Ala Lys Asn Ile Thr Gly Lys Ala Val Met Asn Met Ser Leu
260 265 270
Gly Gly Ser Ala Ser Ala Ala Val Asn Arg Ala Ile Glu Ala Leu Asn
275 280 285
Thr Ala Gly Ile Val Pro Val Val Ala Ala Gly Asn Glu Asn Gln Asp
290 295 300
Ala Ala Asn Thr Ser Pro Ala Ser Ala Pro Asn Ala Ile Thr Val Gly
305 310 315 320
Ala Ile Asp Ala Arg Asn Asp Arg Lys Ala Ser Phe Ser Asn Phe Gly
325 330 335
Lys Val Val Asp Ile Phe Ala Pro Gly Val Asn Ile Leu Ser Val Gly
340 345 350
Ile Ala Ser Asn Thr Ala Thr Asn Thr Leu Ser Gly Thr Ser Met Ala
355 360 365
Ser Pro His Val Ala Gly Leu Ala Ala Tyr Leu Met Gly Leu Glu Gly
370 375 380
Leu Asn Thr Val Lys Ser Leu Thr Asp Arg Met Leu Gln Leu Ala Thr
385 390 395 400
Ala Ser Gly Ala Ser Val Gln Arg Asn Thr Ala Gly Thr Thr Asn Leu
405 410 415
Ile Ala Asn Asn Gly Gln Leu
420
<210> 207
<211> 387
<212> PRT
<213> Nectria haematococca
<400> 207
Met Val Ser Asn Pro Glu Ala Gln Asn Ile Ile Pro Asn Lys Tyr Ile
1 5 10 15
Val Val Phe Asn Asn Asp Asp Phe Asp Asp Asp Ala Ile Ser Ala Lys
20 25 30
Glu Ala Ser Phe Ala Met Ala Ile Lys Lys Arg Asn Leu Asn Lys Arg
35 40 45
Gly Leu Asn Gly Leu Asn Leu Ser Thr Thr Ile Asn Ser Phe Lys Met
50 55 60
Asn Lys Trp Arg Ala Met Ser Leu Asp Ala Asp Asp Leu Met Ile Lys
65 70 75 80
Asp Leu Tyr Asp Ala Asp Glu Val Ala Tyr Ile Glu Ala Asp Thr His
85 90 95
Val Gln Leu Asn Gly Ala Ile Ala Gln Val Asn Ala Pro Pro Gly Leu
100 105 110
Asp Arg Leu Ser His Ala Gln Pro Asn Glu Asp Thr Tyr Val Phe Asp
115 120 125
Asp Ser Ala Gly Glu Gly Ile Val Ala Tyr Val Val Asp Thr Gly Ile
130 135 140
Lys Thr Thr His Thr Glu Phe Glu Gly Arg Ala Thr Phe Gly Gly Asn
145 150 155 160
Phe Ile Asn Asn Val Asp Asp Asp Glu Asn Gly His Gly Ser His Val
165 170 175
Ala Gly Thr Ile Ala Gly Ala Thr Phe Gly Val Ala Lys Lys Ala Glu
180 185 190
Leu Val Ala Val Lys Val Leu Asp Val Ser Gly Gly Gly Ser Asn Ser
195 200 205
Gly Val Leu Gln Gly Met Gln Phe Val Ile Asp Asp Val Glu Lys Arg
210 215 220
Gly Lys Lys Gly Lys Ala Val Met Asn Met Ser Leu Gly Gly Asp Phe
225 230 235 240
Ser Glu Ala Ile Asn Arg Ala Ile Glu Ala Leu Phe Lys Ala Gly Ile
245 250 255
Val Pro Val Val Ala Ala Gly Asn Glu Asn Val Asn Thr Ala Leu Thr
260 265 270
Ser Pro Gly Ser Ala Pro Asn Ala Ile Thr Val Gly Ala Ile Asp Gly
275 280 285
Thr Thr Asp Val Arg Ala Pro Phe Ser Asn Phe Gly Pro Asp Val Asp
290 295 300
Ile Tyr Ala Pro Gly Val Gln Val Leu Ser Val Gly Ile Asp Ser Asn
305 310 315 320
Thr Ala Thr Ala Ser Leu Ser Gly Thr Ser Met Ala Ser Pro His Val
325 330 335
Ala Gly Leu Ala Ala Tyr Leu Met Ala Phe Gln Asn Val Gln Glu Pro
340 345 350
Ser Arg Val Val Glu Leu Ile Lys Ser Leu Ala Gln Gln Ser Gly Ala
355 360 365
Lys Val Arg Asn Asn Ala Ala Asp Thr Thr Asp Leu Ile Ala Asn Asn
370 375 380
Gly Asn Gln
385
<210> 208
<211> 425
<212> PRT
<213> 蛹虫草
<400> 208
Met Thr Gln Leu His Tyr Arg Phe Thr Ser Leu Leu Leu Leu Leu Gly
1 5 10 15
Leu Ala Ile Ala Ala Pro Gly Arg Ile Ala Asn Pro Pro Glu Ser Thr
20 25 30
Ala Phe Arg Leu Pro Val Ser Asn Ala Asn Val Ala Val Thr Asn Met
35 40 45
Ile Pro Asn Arg Tyr Ile Val Val Tyr Asn Lys Asp Phe Asn Ser Thr
50 55 60
Ser Ile Asp Ala Lys Glu Ala Ser Phe Ala Ala Ala Leu Arg Lys Arg
65 70 75 80
Asn Leu Gly Lys Arg Gly Leu Gly Gly Asn Pro Leu Ser Thr Glu Val
85 90 95
Asn Ser Leu Ser Met Asn Lys Trp Arg Ala Ser Ile Leu Asp Ala Asp
100 105 110
Asp Asp Met Phe Met Glu Met Tyr Asn Ser Glu Glu Val Ser Tyr Ile
115 120 125
Glu Ala Asp Thr Arg Val His Thr Gln Val Val Ile Ser Gln Ile Asn
130 135 140
Ala Pro Pro Gly Leu Ala Arg Leu Ser His Ala Thr Asn Gly Pro Ser
145 150 155 160
Asn Tyr Leu Phe Asp Glu Ser Ala Gly Gln Gly Val Thr Val Tyr Ile
165 170 175
Val Asp Thr Gly Ile Leu Pro Ser His Val Glu Phe Glu Gly Arg Ala
180 185 190
Arg Ser Ala Ala Asn Phe Val Asn Gln Val Asp Thr Asp Glu Asn Gly
195 200 205
His Gly Ser His Val Ala Gly Thr Ile Gly Gly Ala Thr Tyr Gly Val
210 215 220
Ala Lys Arg Cys Glu Leu Leu Gly Val Lys Val Leu Asp Ala Ser Gly
225 230 235 240
Gly Gly Ala Asn Ser Gly Val Leu Lys Gly Leu Gln Phe Val Met Asn
245 250 255
Asp Val Ala Glu Arg Asn Val Ser Lys Ala Val Met Asn Met Ser Leu
260 265 270
Gly Gly Pro Arg Ser Arg Ala Ile Asn Thr Ala Ile Ser Gln Leu Ala
275 280 285
Gln Ala Gly Val Ile Pro Val Val Ala Ala Gly Asn Glu Asp Gln Asp
290 295 300
Ala Ser Thr Thr Ser Pro Ala Ser Ala Pro Asp Ala Ile Thr Val Gly
305 310 315 320
Ala Ile Asp Val Arg Asn Asp Ala Lys Ala Ser Phe Ser Asn Phe Gly
325 330 335
Ser Ser Val Ala Ile Phe Ala Pro Gly Val Asn Ile Leu Ser Val Gly
340 345 350
Ile Ala Ser Asn Thr Asp Ser Lys Val Leu Ser Gly Thr Ser Met Ala
355 360 365
Ser Pro His Val Ala Gly Leu Ala Ala Tyr Leu Met Gly Leu Arg Gly
370 375 380
Leu Asp Glu Gln Gln Pro Ser Gln Val Glu Asp Leu Met Lys Asn Leu
385 390 395 400
Ala Gly Gln Thr Gly Ala Arg Val Arg Gly Asn Glu Gln Gly Thr Thr
405 410 415
Asn Arg Ile Ala Asn Asn Gly Ser Arg
420 425
<210> 209
<211> 433
<212> PRT
<213> 粗糙链孢霉
<400> 209
Met Val Arg Phe Ser Val Ala Ala Ala Phe Leu Leu Ser Ala Leu Gly
1 5 10 15
Val Thr Ala Ala Pro Ser Gly Gly Arg His Asn His Gln Asn Thr Gln
20 25 30
Asn Thr Gly Ala Thr Ala Gly Asn Ala Ala Gly Val Pro Val Ala Asn
35 40 45
Ser Asp Ile Ser Asn Ile Ile Pro Gly Arg Tyr Ile Val Val Tyr Asn
50 55 60
Asn Thr Phe Gly Glu Glu Ala Ile Asn Ala His Gln Ile Lys Val Thr
65 70 75 80
Ser Leu Val Ala Lys Arg Asn Leu Gly Lys Arg Asp Ala Lys Thr Gly
85 90 95
Arg Ile Met Ser Pro Ser Val Lys Ala Phe Lys Met Gly Thr Trp Arg
100 105 110
Ala Met Ala Leu Asp Ala Asp Asp Asp Met Ile Asn Asp Ile Asn Ser
115 120 125
Ala Gln Glu Val Glu Tyr Ile Glu Ala Asp Gln Tyr Val Lys Leu Asn
130 135 140
Ala Leu Thr Ser Gln Asn Ser Thr Thr Thr Gly Leu Ala Arg Leu Ser
145 150 155 160
His Ala Gly Pro Ser Lys Lys Ala Ala Pro Tyr Ile Phe Asp Ser Ser
165 170 175
Ala Gly Glu Gly Ile Thr Ala Phe Val Val Asp Thr Gly Ile Arg Val
180 185 190
Thr His Ser Glu Tyr Glu Gly Arg Ala Thr Phe Ala Ala Asn Phe Val
195 200 205
Asn Asn Val Asp Thr Asp Glu Asn Gly His Gly Ser His Val Ala Gly
210 215 220
Thr Ile Ala Gly Ala Thr Phe Gly Val Ala Lys Lys Ala Lys Leu Val
225 230 235 240
Ala Val Lys Val Leu Asp Gly Ser Gly Ser Gly Ser Asn Ser Gly Val
245 250 255
Leu Gln Gly Met Gln Phe Val Ala Asp Thr Ala Thr Ser Gln Lys Leu
260 265 270
Gly Gly Lys Ala Val Leu Asn Met Ser Leu Gly Gly Gly Lys Ser Arg
275 280 285
Ala Ile Asn Ser Ala Ile Asn Gln Ile Ala Ala Ala Gly Val Val Pro
290 295 300
Val Val Ala Ala Gly Asn Glu Asn Gln Asp Thr Ala Asn Thr Ser Pro
305 310 315 320
Gly Ser Ala Pro Ala Ala Ile Thr Val Gly Ala Ile Asp Gln Arg Thr
325 330 335
Asp Ala Arg Ala Ser Phe Ser Asn Phe Gly Ala Gly Val Asp Ile Phe
340 345 350
Ala Pro Gly Val Asn Val Leu Ser Val Gly Ile Lys Ser Asp Thr Asp
355 360 365
Thr Asp Thr Leu Ser Gly Thr Ser Met Ala Ser Pro His Val Ala Gly
370 375 380
Leu Ala Ala Tyr Leu Met Ala Leu Glu Gly Leu Thr Asp Val Thr Ala
385 390 395 400
Val Gly Asn Arg Ile Lys Glu Leu Ala Gln Lys Thr Gly Ala Lys Val
405 410 415
Thr Asn Asn Val Arg Gly Thr Thr Ser Leu Ile Ala Asn Asn Gly Asn
420 425 430
Leu
<210> 210
<211> 433
<212> PRT
<213> 四孢链孢菌
<400> 210
Met Val Arg Phe Ser Val Ala Ala Ala Phe Leu Leu Ser Ala Leu Gly
1 5 10 15
Val Thr Ala Ala Pro Ser Gly Gly Arg His Asn His Gln Asn Thr Gln
20 25 30
Asn Thr Gly Ala Thr Ala Gly Asn Ala Ala Gly Val Pro Val Ser Asn
35 40 45
Ser Asp Ile Ser Asn Ile Ile Pro Gly Arg Tyr Ile Val Val Tyr Asn
50 55 60
Asn Thr Phe Gly Glu Glu Ala Ile Ser Ala His Gln Ile Lys Val Thr
65 70 75 80
Ser Leu Val Ala Lys Arg Asn Leu Gly Lys Arg Asp Ala Lys Thr Gly
85 90 95
Arg Ile Met Ser Pro Ser Val Lys Ala Phe Lys Met Gly Thr Trp Arg
100 105 110
Ala Met Ala Leu Asp Ala Asp Asp Asp Met Ile Asn Asp Ile Asn Ser
115 120 125
Ala Gln Glu Val Glu Tyr Ile Glu Ala Asp Gln Tyr Val Lys Leu Asn
130 135 140
Ala Leu Thr Ser Gln Asn Ser Thr Thr Thr Gly Leu Ala Arg Leu Ser
145 150 155 160
His Ala Gly Pro Ser Lys Gln Ala Ala Pro Tyr Ile Phe Asp Ser Ser
165 170 175
Ala Gly Glu Gly Ile Thr Ala Phe Val Val Asp Thr Gly Ile Arg Val
180 185 190
Thr His Ser Glu Tyr Glu Gly Arg Ala Thr Phe Ala Ala Asn Phe Val
195 200 205
Asn Asn Val Asp Thr Asp Glu Asn Gly His Gly Ser His Val Ala Gly
210 215 220
Thr Ile Ala Gly Ala Thr Phe Gly Val Ala Lys Lys Ala Lys Leu Val
225 230 235 240
Ala Val Lys Val Leu Asp Gly Ser Gly Ser Gly Ser Asn Ser Gly Val
245 250 255
Leu Gln Gly Met Gln Phe Val Ala Asp Thr Ala Thr Ser Gln Lys Leu
260 265 270
Gly Gly Lys Ala Val Leu Asn Met Ser Leu Gly Gly Gly Lys Ser Arg
275 280 285
Ala Ile Asn Ser Ala Ile Asn Gln Ile Ala Ala Ala Gly Val Val Pro
290 295 300
Val Val Ala Ala Gly Asn Glu Asn Gln Asp Thr Ala Asn Thr Ser Pro
305 310 315 320
Gly Ser Ala Pro Ala Ala Ile Thr Val Gly Ala Ile Asp Gln Arg Thr
325 330 335
Asp Ala Arg Ala Ser Phe Ser Asn Phe Gly Ala Gly Val Asp Ile Phe
340 345 350
Ala Pro Gly Val Asn Val Leu Ser Val Gly Ile Thr Ser Asp Thr Ala
355 360 365
Thr Asp Thr Leu Ser Gly Thr Ser Met Ala Ser Pro His Val Ala Gly
370 375 380
Leu Ala Ala Tyr Leu Met Ala Leu Glu Gly Leu Thr Asp Val Thr Ala
385 390 395 400
Val Gly Asn Arg Ile Lys Glu Leu Ala Gln Lys Thr Gly Ala Lys Val
405 410 415
Thr Asn Asn Val Arg Gly Thr Thr Ser Leu Ile Ala Asn Asn Gly Asn
420 425 430
Leu
<210> 211
<211> 411
<212> PRT
<213> 大丽轮枝菌
<400> 211
Met Ala Pro Ile Ser Lys Glu Val Pro Ala Gly Ala Ile Thr Gly Ala
1 5 10 15
Ala Asp Phe Phe Ala Lys Gly Phe Ser Ala Met Leu Asn Ser Glu Ser
20 25 30
Thr Glu Arg Val Ser Asn Ser Tyr Ile Val Val Tyr Asn Asn Thr Phe
35 40 45
Asp Asp Asp Thr Ile Asn Phe Arg Gln Thr Glu Val Met Asn His Ile
50 55 60
Lys Arg Arg Asn Ile Gly Lys Arg Gly Leu Asp Gly Arg Gln Leu Ser
65 70 75 80
Thr Glu Val His Ala Leu Ala Met Asn Gly Trp Arg Ala Met Thr Leu
85 90 95
Glu Ser Asp Asp Asp Met Ile Leu Gln Ile Met Asn Glu Pro Glu Val
100 105 110
Ala Trp Val Glu Ala Asn Ala Arg Val Lys Leu Ser Ala Ser Val Ala
115 120 125
Gln Met Asn Ala Pro Pro Gly Leu Asn Arg Leu Ser Asn Ala Gln Ala
130 135 140
Gly Thr Gly Asn Tyr Val Phe Asp Thr Thr Gly Gly Glu Gly Val Thr
145 150 155 160
Val Tyr Val Val Asp Thr Gly Ile Arg Thr Asp His Ser Glu Phe Gln
165 170 175
Gly Arg Ala Thr Phe Gly Val Asn Thr Val Asp Asn Val Asp Asn Asp
180 185 190
Gln Asn Gly His Gly Ser His Val Ala Gly Thr Ile Cys Gly Gln Thr
195 200 205
Phe Gly Val Ala Lys Ser Ala Asn Val Val Ala Val Lys Val Leu Asp
210 215 220
Gly Thr Gly Ala Gly Ser Asn Ala Gly Val Leu Asp Gly Leu Gln Phe
225 230 235 240
Ile Ile Asn Asp Val Gln Gln Lys Asn Leu Arg Gly Lys Ala Val Met
245 250 255
Asn Met Ser Leu Gly Gly Pro Gln Ser Ala Ala Val Asn Arg Ala Val
260 265 270
Gln Ala Leu Phe Asp Ala Gly Val Val Pro Val Val Ala Ala Gly Asn
275 280 285
Glu Asn Gln Asp Ala Ala Asn Thr Ser Pro Ala Ser Ala Pro Lys Ala
290 295 300
Ile Thr Val Gly Ala Ile Asp Ala Ser Asn Asp Gln Lys Ala Ser Phe
305 310 315 320
Ser Asn Phe Gly Ala Asp Val Asp Ile Phe Ala Pro Gly Val Asp Val
325 330 335
Leu Ser Val Gly Ile Arg Ser Asn Thr Ala Thr Asp Thr Leu Ser Gly
340 345 350
Thr Ser Met Ala Ser Pro His Val Ala Gly Leu Ala Ala Tyr Leu Ile
355 360 365
Ala Leu Glu Asn Ile Asn Thr Pro Glu Ala Val Ala Ser Arg Leu Thr
370 375 380
Glu Leu Ala Thr Ala Ser Gly Ala Gln Val Leu Arg Asn Thr Pro Gly
385 390 395 400
Thr Thr Asn Leu Ile Ala Asn Asn Gly Gln Leu
405 410
<210> 212
<211> 424
<212> PRT
<213> 太瑞斯梭孢壳霉
<400> 212
Met Ala Gly Arg Leu Leu Ala Ala Leu Thr Phe Ala Leu Ala Ala Leu
1 5 10 15
Gly Ala Ser Gly Leu Pro Gln Pro Ser Lys Ala Glu Gly Phe Phe Leu
20 25 30
Gly Val Pro Ile Ser Asn Pro Glu Ala Lys Asn Ala Ile Pro His Lys
35 40 45
Tyr Ile Val Val Tyr Asn Ser Thr Phe Ser Asp Ala Glu Ile Gln Ala
50 55 60
His Gln Asp Ser Ile Ile Lys Thr Ile Ser Lys Arg Asn Val Gly Lys
65 70 75 80
Arg Ser Pro Leu Thr Gly Lys Val Leu Ser Pro His Val Ser Thr Phe
85 90 95
Ser Met Gly Thr Trp Arg Ala Met Ala Leu Glu Ala Asp Asp Leu Met
100 105 110
Met Asn Glu Ile Phe Ala Ala Lys Glu Val Asp Tyr Ile Glu Gln Asp
115 120 125
Ala Tyr Val Ser Ile Ala Ala Gln Gln Val Gln Ser Gln Ala Thr Ser
130 135 140
Gly Leu Ala Arg Ile Ser His Ala Lys Pro Gly Ala Thr Thr Tyr Val
145 150 155 160
Phe Asp Ser Ser Ala Gly Gln Gly Met Thr Ala Tyr Val Val Asp Thr
165 170 175
Gly Ile Leu Val Thr His Ser Glu Phe Glu Gly Arg Ala Thr Phe Ala
180 185 190
Ala Asn Phe Val Gly Asp Asn Gln Asp Thr Asp Leu Asn Gly His Gly
195 200 205
Ser His Val Ala Gly Thr Ile Gly Gly Lys Thr Phe Gly Val Ala Lys
210 215 220
Lys Val Asn Leu Val Ala Val Lys Val Leu Asp Ala Asn Gly Ser Gly
225 230 235 240
Thr Asn Ser Gly Val Ile Ala Gly Met Gln Phe Val Ala Gln Asn Ala
245 250 255
Thr Gly Ser Gly Leu Ser Gly Lys Ala Val Met Asn Met Ser Val Gly
260 265 270
Gly Ser Phe Ser Arg Ala Val Asn Ser Ala Ile Asn Gln Val Ala Ala
275 280 285
Ala Gly Val Val Pro Val Val Ala Ala Gly Asn Glu Asn Gln Asn Ala
290 295 300
Ala Asn Thr Ser Pro Ala Ser Ala Thr Ala Ala Ile Thr Val Gly Ala
305 310 315 320
Ile Asp Gln Thr Asp Asp Thr Arg Ala Ser Phe Ser Asn Phe Gly Arg
325 330 335
Val Val Asp Val Phe Ala Pro Gly Val Asn Val Leu Ser Val Gly Ile
340 345 350
Arg Ser Asn Thr Asp Thr Gln Val Leu Ser Gly Thr Ser Met Ala Thr
355 360 365
Pro His Val Ala Gly Leu Ala Ala Tyr Leu Met Ala Leu Glu Gly Ile
370 375 380
Thr Gly Gly Ala Gln Ala Val Ser Asp Arg Ile Lys Gln Leu Ala Gln
385 390 395 400
Ala Thr Gly Ala Thr Val Gly Asn Asp Val Arg Gly Thr Thr Thr Leu
405 410 415
Ile Ala Asn Asn Gly Asn Arg Asn
420
<210> 213
<211> 511
<212> PRT
<213> 稻瘟病菌
<400> 213
Met Ala Pro Ile Ser Ser Ile Leu Ala Pro Phe Ala Ala Leu Ala Gly
1 5 10 15
Leu Val Ala Ala Leu Pro Ser Asn Phe Pro Gly Ser Glu Lys Phe Ala
20 25 30
Gly Val Pro Val Ser Asn Ile Asn Ala Leu Asn Val Ile Pro Gln Ser
35 40 45
Tyr Ile Val Val Tyr Asn Ser Thr Phe Asp Ser Lys Ile Ile Asp Asp
50 55 60
Ser Gln Ala Met Ala Met Ala Lys Val Ala Glu Arg Asn Leu Gly Lys
65 70 75 80
Arg Ser Glu Asp Gly Arg Asp Met Ser Thr Thr Ala Thr Thr Cys Gln
85 90 95
Ile Gly Thr Trp Arg Gly Leu Met Leu Asp Ala Asp Asp Gln Thr Ile
100 105 110
Met Glu Ile Met Ala Asp Pro Ala Val Arg Tyr Val Glu Ala Asp Ala
115 120 125
Ala Met Asn Ile Leu Ile Asp Glu Glu Ala Ser Glu Met Pro Pro Pro
130 135 140
Ala Glu Ile Thr Glu Met Gln Ala Ile Ala Arg Arg Ala Glu Gln Thr
145 150 155 160
Glu Thr Gly Ala Pro Asn Gly Leu Ala Arg Leu Ser Gln Ser Glu Pro
165 170 175
Asn Pro Gly Thr Ser Gly Thr Tyr Arg Phe Glu Asp Ser Ala Gly Glu
180 185 190
Gly Ile Thr Val Tyr Ile Val Asp Thr Gly Ile Arg Ala Thr His Thr
195 200 205
Glu Phe Glu Asp Arg Ala Thr Phe Gly Ala Asn Phe Ile Asp Gln Val
210 215 220
Asp Thr Asp Gln Asn Gly His Gly Ser His Cys Ala Gly Thr Ile Gly
225 230 235 240
Gly Lys Thr Phe Gly Val Ala Lys Lys Ala Thr Leu Val Gly Val Lys
245 250 255
Val Leu Gly Ala Ser Gly Gly Gly Ser Asn Arg Gly Val Ile Gln Gly
260 265 270
Met Gln Phe Val Ala Asn Asp Val Thr Gln Lys Asn Leu Ala Arg Lys
275 280 285
Ala Val Met Asn Met Ser Leu Gly Gly Ser Lys Ser Thr Ala Val Asn
290 295 300
Glu Ala Ile Asn Ser Met Ile Lys Ala Gly Val Val Ala Val Val Ala
305 310 315 320
Ala Gly Asn Glu Asn Asp Asp Ala Ser Lys Ser Ser Pro Ala Ser Ala
325 330 335
Pro Gly Ala Ile Thr Val Gly Ala Ile Asp Gln Lys Asn Asp Arg Lys
340 345 350
Ala Ser Phe Ser Asn Phe Gly Thr Asp Val Asp Ile Phe Ala Pro Gly
355 360 365
Val Asn Val Leu Ser Val Asp Ala Lys Ser Asp Thr Gly Ser Lys Thr
370 375 380
Leu Ser Gly Thr Ser Met Ala Ser Pro His Val Ala Gly Leu Ala Ala
385 390 395 400
Tyr Leu Met Ala Leu Asp Pro Thr Leu Thr Ala Gly Gly Gly Ile Glu
405 410 415
Thr Val Ala Gly Lys Val Asp Ala Arg Ile Lys Glu Leu Ala Lys Ala
420 425 430
Met Gly Ala Gln Val Gly Gln Ala Pro Ala Gly Thr Thr Thr Leu Ile
435 440 445
Ala Asn Asn Gly Lys Thr Gly Asn Gly Ala Gly Asn Gly Thr Ala Ala
450 455 460
Ala Ala Pro Ala Asp Asn Ala Asp Ala Ala Pro Ala Thr Gly Ala Thr
465 470 475 480
Arg Arg Leu Arg Lys Ala Gln Arg Leu Thr Phe Gln Arg Met Gln Gln
485 490 495
Phe Gln Arg Gln Gln Ile Gln Arg Gln Gln Ser Gln Arg Gln Gln
500 505 510
<210> 214
<211> 409
<212> PRT
<213> 里氏木霉
<400> 214
Met Ala Ser Leu Arg Arg Leu Ala Leu Tyr Leu Gly Ala Leu Leu Pro
1 5 10 15
Ala Val Leu Ala Ala Pro Ala Val Asn Tyr Lys Leu Pro Glu Ala Val
20 25 30
Pro Asn Lys Phe Ile Val Thr Leu Lys Asp Gly Ala Ser Val Asp Thr
35 40 45
Asp Ser His Leu Thr Trp Val Lys Asp Leu His Arg Arg Ser Leu Gly
50 55 60
Lys Arg Ser Thr Ala Gly Val Glu Lys Thr Tyr Asn Ile Asp Ser Trp
65 70 75 80
Asn Ala Tyr Ala Gly Glu Phe Asp Glu Glu Thr Val Lys Gln Ile Lys
85 90 95
Ala Asn Pro Asp Val Ala Ser Val Glu Pro Asp Tyr Ile Met Trp Leu
100 105 110
Ser Asp Ile Val Glu Asp Lys Arg Ala Leu Thr Thr Gln Thr Gly Ala
115 120 125
Pro Trp Gly Leu Gly Thr Val Ser His Arg Thr Pro Gly Ser Thr Ser
130 135 140
Tyr Ile Tyr Asp Thr Ser Ala Gly Ser Gly Thr Phe Ala Tyr Val Val
145 150 155 160
Asp Ser Gly Ile Asn Ile Ala His Gln Gln Phe Gly Gly Arg Ala Ser
165 170 175
Leu Gly Tyr Asn Ala Ala Gly Gly Asp His Val Asp Thr Leu Gly His
180 185 190
Gly Thr His Val Ser Gly Thr Ile Gly Gly Ser Thr Tyr Gly Val Ala
195 200 205
Lys Gln Ala Ser Leu Ile Ser Val Lys Val Phe Gln Gly Asn Ser Ala
210 215 220
Ser Thr Ser Val Ile Leu Asp Gly Tyr Asn Trp Ala Val Asn Asp Ile
225 230 235 240
Val Ser Arg Asn Arg Ala Ser Lys Ser Ala Ile Asn Met Ser Leu Gly
245 250 255
Gly Pro Ala Ser Ser Thr Trp Ala Thr Ala Ile Asn Ala Ala Phe Asn
260 265 270
Lys Gly Val Leu Thr Ile Val Ala Ala Gly Asn Gly Asp Ala Leu Gly
275 280 285
Asn Pro Gln Pro Val Ser Ser Thr Ser Pro Ala Asn Val Pro Asn Ala
290 295 300
Ile Thr Val Ala Ala Leu Asp Ile Asn Trp Arg Thr Ala Ser Phe Thr
305 310 315 320
Asn Tyr Gly Ala Gly Val Asp Val Phe Ala Pro Gly Val Asn Ile Leu
325 330 335
Ser Ser Trp Ile Gly Ser Asn Thr Ala Thr Asn Thr Ile Ser Gly Thr
340 345 350
Ser Met Ala Thr Pro His Val Val Gly Leu Ala Leu Tyr Leu Gln Ala
355 360 365
Leu Glu Gly Leu Ser Thr Pro Thr Ala Val Thr Asn Arg Ile Lys Ala
370 375 380
Leu Ala Thr Thr Gly Arg Val Thr Gly Ser Leu Asn Gly Ser Pro Asn
385 390 395 400
Thr Leu Ile Phe Asn Gly Asn Ser Ala
405
<210> 215
<211> 409
<212> PRT
<213> 深绿木霉
<400> 215
Met Thr Ser Ile Arg Arg Leu Ala Leu Tyr Leu Gly Ala Leu Leu Pro
1 5 10 15
Ala Val Leu Ala Ala Pro Ala Ala Leu His Lys Lys Pro Glu Ala Val
20 25 30
Pro Asn Lys Phe Ile Val Thr Leu Lys Glu Gly Ala Ser Ile Asp Thr
35 40 45
Asp Ser His Leu Ala Trp Val Thr Asp Ile His Thr Arg Ser Leu Thr
50 55 60
Lys Arg Ser Thr Ala Gly Val Glu Lys Thr Tyr Asn Ile His Thr Trp
65 70 75 80
Asn Ala Tyr Ala Gly Glu Phe Asp Glu Glu Thr Ile Glu Gln Ile Lys
85 90 95
Ser Asn Pro Asp Val Ala Ser Val Glu Pro Asp Tyr Ile Met His Leu
100 105 110
Ser Asp Ile Val Glu Asp Lys Arg Ala Leu Thr Thr Gln Ser Gly Ala
115 120 125
Pro Trp Gly Leu Gly Thr Val Ser His Arg Thr Ser Gly Ser Thr Ser
130 135 140
Tyr Ile Tyr Asp Ser Ser Ala Gly Ala Gly Thr Phe Ala Tyr Val Val
145 150 155 160
Asp Ser Gly Ile Asn Thr Ser His Gln Gln Phe Gly Gly Arg Ala Ser
165 170 175
Leu Gly Tyr Asn Ala Ala Gly Gly Gln His Val Asp Thr Leu Gly His
180 185 190
Gly Thr His Val Ser Gly Thr Ile Gly Gly Ser Thr Tyr Gly Val Ala
195 200 205
Lys Gln Ala Ser Leu Ile Ser Val Lys Val Phe Ala Gly Glu Ser Ala
210 215 220
Ser Thr Ser Val Ile Leu Asp Gly Tyr Asn Trp Ala Val Asn Asp Ile
225 230 235 240
Val Ser Lys Ser Arg Ala Ser Lys Ser Ala Ile Asn Met Ser Leu Gly
245 250 255
Gly Pro Ala Ser Ser Thr Trp Thr Thr Ala Ile Asn Ala Ala Phe Asn
260 265 270
Gln Gly Val Leu Thr Ile Val Ala Ala Gly Asn Gly Asp Ser Leu Gly
275 280 285
Asn Pro Gln Pro Val Ser Gly Thr Ser Pro Ala Asn Val Pro Asn Ala
290 295 300
Ile Thr Val Ala Ala Leu Asp Ile Asn Trp Arg Thr Ala Ser Phe Thr
305 310 315 320
Asn Tyr Gly Ala Gly Val Asp Val Phe Ala Pro Gly Val Asn Ile Leu
325 330 335
Ser Ser Trp Ile Gly Ser Asn Thr Ala Thr Asn Thr Ile Ser Gly Thr
340 345 350
Ser Met Ala Thr Pro His Val Val Gly Leu Ala Leu Tyr Leu Gln Ser
355 360 365
Leu Glu Gly Leu Thr Ser Pro Thr Ala Val Thr Asn Arg Ile Lys Ala
370 375 380
Leu Ala Thr Ser Gly Arg Val Thr Gly Ser Leu Asn Gly Ser Pro Asn
385 390 395 400
Val Ile Ile Phe Asn Gly Asn Ser Ser
405
<210> 216
<211> 409
<212> PRT
<213> 绿木霉
<400> 216
Met Ala Ser Ile Arg Arg Leu Ala Leu Tyr Leu Gly Ala Leu Leu Pro
1 5 10 15
Ala Val Leu Ala Ala Pro Ala Gly His His Lys Ala Pro Glu Ala Val
20 25 30
Pro Asn Lys Phe Ile Val Thr Leu Lys Glu Gly Ala Ser Ile Asp Thr
35 40 45
Asp Ser His Leu Thr Trp Val Asn Asp Leu His Arg Arg Ser Leu Thr
50 55 60
Lys Arg Asn Thr Ala Gly Val Glu Lys Thr Tyr Asn Ile His Thr Trp
65 70 75 80
Ser Ala Tyr Ala Gly Glu Phe Asp Glu Asp Thr Ile Glu Gln Ile Lys
85 90 95
Ala Ser Pro Asp Val Ala Ser Val Glu Pro Asp Tyr Ile Met Tyr Leu
100 105 110
Ser Asp Ile Val Glu Asp Lys Arg Ala Leu Thr Thr Gln Thr Gly Ala
115 120 125
Pro Trp Gly Leu Gly Thr Val Ser His Arg Thr Ser Gly Ser Thr Ser
130 135 140
Tyr Ile Tyr Asp Thr Ser Ala Gly Ser Gly Thr Phe Ala Tyr Val Val
145 150 155 160
Asp Ser Gly Ile Asn Thr Ser His Gln Gln Phe Gly Gly Arg Ala Ser
165 170 175
Leu Gly Tyr Asn Ala Ala Gly Gly Gln His Val Asp Thr Leu Gly His
180 185 190
Gly Thr His Val Ser Gly Thr Ile Gly Gly Ser Thr Tyr Gly Val Ala
195 200 205
Lys Gln Ala Ser Leu Ile Ser Val Lys Val Phe Gln Gly Asn Ser Ala
210 215 220
Ser Thr Ser Val Ile Leu Asp Gly Tyr Asn Trp Ala Val Asn Asp Ile
225 230 235 240
Val Ser Arg Asn Arg Ala Ser Lys Ser Ala Ile Asn Met Ser Leu Gly
245 250 255
Gly Pro Ala Ser Ser Ala Trp Thr Thr Ala Ile Asn Ala Ala Phe Asn
260 265 270
Gln Gly Val Leu Thr Ile Val Ala Ala Gly Asn Gly Asp Ser Leu Gly
275 280 285
Asn Pro Gln Pro Val Ser Gly Thr Ser Pro Ala Asn Val Pro Asn Ala
290 295 300
Ile Thr Val Ala Ala Leu Asp Ile Asn Trp Arg Thr Ala Ser Phe Thr
305 310 315 320
Asn Tyr Gly Ala Gly Val Asp Val Phe Ala Pro Gly Val Asn Ile Leu
325 330 335
Ser Ser Trp Ile Gly Ser Asn Thr Ala Thr Asn Thr Ile Ser Gly Thr
340 345 350
Ser Met Ala Thr Pro His Val Val Gly Leu Ala Leu Tyr Leu Gln Ser
355 360 365
Leu Glu Gly Leu Ala Thr Pro Thr Ala Val Thr Asn Arg Ile Lys Ala
370 375 380
Leu Ala Thr Ser Gly Arg Val Thr Gly Ser Leu Asn Gly Ser Pro Asn
385 390 395 400
Val Leu Ile Phe Asn Gly Asn Ser Ala
405
<210> 217
<211> 409
<212> PRT
<213> 绿木霉
<400> 217
Met Ala Ser Ile Arg Arg Leu Ala Leu Tyr Leu Gly Ala Leu Leu Pro
1 5 10 15
Ala Val Leu Ala Ala Pro Ala Gly His His Lys Ala Pro Glu Ala Val
20 25 30
Pro Asn Lys Phe Ile Val Thr Leu Lys Glu Gly Ala Ser Ile Asp Thr
35 40 45
Asp Ser His Leu Thr Trp Val Asn Asp Leu His Arg Arg Ser Leu Thr
50 55 60
Lys Arg Asn Thr Ala Gly Val Glu Lys Thr Tyr Asn Ile His Thr Trp
65 70 75 80
Ser Ala Tyr Ala Gly Glu Phe Asp Glu Asp Thr Ile Glu Gln Ile Lys
85 90 95
Ala Ser Pro Asp Val Ala Ser Val Glu Pro Asp Tyr Ile Met Tyr Leu
100 105 110
Ser Asp Ile Val Glu Asp Lys Arg Ala Leu Thr Thr Gln Thr Gly Ala
115 120 125
Pro Trp Gly Leu Gly Thr Val Ser His Arg Thr Ser Gly Ser Thr Ser
130 135 140
Tyr Ile Tyr Asp Thr Ser Ala Gly Ser Gly Thr Phe Ala Tyr Val Val
145 150 155 160
Asp Ser Gly Ile Asn Thr Ser His Gln Gln Phe Gly Gly Arg Ala Ser
165 170 175
Leu Gly Tyr Asn Ala Ala Gly Gly Gln His Val Asp Thr Leu Gly His
180 185 190
Gly Thr His Val Ser Gly Thr Ile Gly Gly Ser Thr Tyr Gly Val Ala
195 200 205
Lys Gln Ala Ser Leu Ile Ser Val Lys Val Phe Gln Gly Asn Ser Ala
210 215 220
Ser Thr Ser Val Ile Leu Asp Gly Tyr Asn Trp Ala Val Asn Asp Ile
225 230 235 240
Val Ser Arg Asn Arg Ala Ser Lys Ser Ala Ile Asn Met Ser Leu Gly
245 250 255
Gly Pro Ala Ser Ser Ala Trp Thr Thr Ala Ile Asn Ala Ala Phe Asn
260 265 270
Gln Gly Val Leu Thr Ile Val Ala Ala Gly Asn Gly Asp Ser Leu Gly
275 280 285
Asn Pro Gln Pro Val Ser Gly Thr Ser Pro Ala Asn Val Pro Asn Ala
290 295 300
Ile Thr Val Ala Ala Leu Asp Ile Asn Trp Arg Thr Ala Ser Phe Thr
305 310 315 320
Asn Tyr Gly Ala Gly Val Asp Val Phe Ala Pro Gly Val Asn Ile Leu
325 330 335
Ser Ser Trp Ile Gly Ser Asn Thr Ala Thr Asn Thr Ile Ser Gly Thr
340 345 350
Ser Met Ala Thr Pro His Val Val Gly Leu Ala Leu Tyr Leu Gln Ser
355 360 365
Leu Glu Gly Leu Ala Thr Pro Thr Ala Val Thr Asn Ser Pro Asn Arg
370 375 380
Ile Lys Ala Leu Ala Thr Ser Gly Arg Val Thr Gly Ser Leu Asn Gly
385 390 395 400
Val Leu Ile Phe Asn Gly Asn Ser Ala
405
<210> 218
<211> 409
<212> PRT
<213> 有性哈茨木霉
<400> 218
Met Ala Ser Ile Arg Arg Leu Ala Leu Tyr Leu Gly Ala Leu Leu Pro
1 5 10 15
Ala Val Leu Ala Ala Pro Ala Ala His His Lys Ala Pro Glu Ala Val
20 25 30
Pro Asn Lys Phe Ile Val Thr Leu Lys Glu Gly Ala Ser Val Asp Thr
35 40 45
Asp Ser His Leu Thr Trp Val Asn Asp Leu His Arg Arg Ser Leu Thr
50 55 60
Lys Arg Asn Thr Ala Gly Val Glu Lys Thr Tyr Asn Ile His Thr Trp
65 70 75 80
Asn Ala Tyr Ala Gly Glu Phe Asp Glu Glu Thr Ile Glu Gln Ile Lys
85 90 95
Ser Ser Pro Asp Val Ala Ser Val Glu Pro Asp Tyr Ile Met Tyr Leu
100 105 110
Ser Asp Ile Val Glu Asp Lys Arg Ala Leu Thr Thr Gln Thr Gly Ala
115 120 125
Pro Trp Gly Leu Gly Thr Val Ser His Arg Thr Ser Gly Ser Thr Ser
130 135 140
Tyr Ile Tyr Asp Thr Ser Ala Gly Ser Gly Thr Tyr Ala Tyr Val Val
145 150 155 160
Asp Ser Gly Ile Asn Thr Ser His Gln Gln Phe Gly Gly Arg Ala Thr
165 170 175
Leu Gly Tyr Asn Ala Ala Gly Gly Gln His Val Asp Thr Leu Gly His
180 185 190
Gly Thr His Val Ser Gly Thr Ile Gly Gly Ser Thr Tyr Gly Val Ala
195 200 205
Lys Gln Ala Asn Leu Val Ser Val Lys Val Phe Gln Gly Asp Ser Ala
210 215 220
Ser Thr Ser Val Ile Leu Asp Gly Tyr Asn Trp Ala Val Asn Asp Ile
225 230 235 240
Val Ser Lys Asn Arg Ala Ser Lys Ser Ala Ile Asn Met Ser Leu Gly
245 250 255
Gly Pro Ala Ser Ser Thr Trp Thr Thr Ala Ile Asn Ala Ala Tyr Asn
260 265 270
Gln Gly Val Leu Thr Ile Val Ala Ala Gly Asn Gly Asp Ser Phe Gly
275 280 285
Asn Pro Gln Pro Val Ser Gly Thr Ser Pro Ala Asn Val Pro Asn Ala
290 295 300
Ile Thr Val Ala Ala Leu Asp Ile Asn Trp Arg Thr Ala Ser Phe Thr
305 310 315 320
Asn Tyr Gly Ala Gly Val Asp Val Phe Ala Pro Gly Val Asn Ile Leu
325 330 335
Ser Ser Trp Ile Gly Ser Asn Thr Ala Thr Asn Thr Ile Ser Gly Thr
340 345 350
Ser Met Ala Thr Pro His Val Val Gly Leu Ala Leu Tyr Leu Gln Ser
355 360 365
Leu Glu Gly Leu Ser Thr Pro Ala Ala Val Thr Asn Arg Ile Lys Ala
370 375 380
Leu Ala Thr Ser Gly Arg Val Thr Gly Ser Leu Asn Gly Ser Pro Asn
385 390 395 400
Val Ile Ile Phe Asn Gly Asn Ser Ala
405
<210> 219
<211> 409
<212> PRT
<213> 钩状木霉
<400> 219
Met Thr Ser Ile Arg Arg Leu Ala Leu Tyr Leu Gly Ala Leu Leu Pro
1 5 10 15
Ala Val Leu Ala Ala Pro Ala Ala Leu His Lys Lys Pro Glu Ala Ala
20 25 30
Pro Asn Lys Phe Ile Val Thr Leu Lys Glu Gly Ala Ser Ile Asp Thr
35 40 45
Asp Ser His Leu Ala Trp Val Asn Asp Ile His Arg Arg Ser Leu Thr
50 55 60
Lys Arg Ser Thr Ala Gly Val Glu Lys Thr Tyr Asn Ile His Thr Trp
65 70 75 80
Ser Ala Tyr Ala Gly Glu Phe Asp Ala Glu Thr Ile Glu Gln Ile Lys
85 90 95
Ser Ser Pro Asp Val Ala Ser Val Glu Pro Asp Tyr Ile Met Tyr Leu
100 105 110
Ser Asp Ile Val Glu Asp Lys Arg Ala Leu Thr Thr Gln Ser Gly Ala
115 120 125
Pro Trp Gly Leu Gly Thr Val Ser His Arg Thr Ser Gly Ser Thr Ser
130 135 140
Tyr Ile Tyr Asp Ser Ser Ala Gly Ser Gly Thr Phe Ala Tyr Val Val
145 150 155 160
Asp Ser Gly Ile Asn Thr Ser His Gln Gln Phe Gly Gly Arg Ala Ser
165 170 175
Leu Gly Tyr Asn Ala Ala Gly Gly Gln His Val Asp Thr Leu Gly His
180 185 190
Gly Thr His Val Ser Gly Thr Ile Gly Gly Ser Thr Tyr Gly Val Ala
195 200 205
Lys Gln Ala Ser Leu Ile Ser Val Lys Val Phe Ala Gly Glu Ser Ala
210 215 220
Thr Thr Ser Val Ile Leu Asp Gly Tyr Asn Trp Ala Val Asn Asp Ile
225 230 235 240
Val Ser Arg Asn Arg Ala Ser Lys Ser Ala Ile Asn Met Ser Leu Gly
245 250 255
Gly Pro Ala Ser Ser Thr Trp Thr Thr Ala Ile Asn Ala Ala Phe Asn
260 265 270
Gln Gly Val Leu Thr Ile Val Ala Ala Gly Asn Gly Asp Ser Phe Gly
275 280 285
Asn Pro Gln Pro Val Ser Gly Thr Ser Pro Ala Asn Val Pro Asn Ala
290 295 300
Ile Thr Val Ala Ala Leu Asp Ile Asn Trp Arg Thr Ala Ser Phe Thr
305 310 315 320
Asn Tyr Gly Ala Gly Val Asp Val Phe Ala Pro Gly Val Asn Ile Leu
325 330 335
Ser Ser Trp Ile Gly Ser Asn Thr Ala Thr Asn Thr Ile Ser Gly Thr
340 345 350
Ser Met Ala Thr Pro His Val Val Gly Leu Ala Leu Tyr Leu Gln Ser
355 360 365
Leu Glu Gly Leu Thr Thr Pro Thr Ala Val Thr Asn Arg Ile Lys Ala
370 375 380
Leu Ala Thr Ala Gly Arg Ile Thr Gly Ser Leu Asn Gly Ser Pro Asn
385 390 395 400
Thr Leu Ile Phe Asn Gly Asn Ser Ala
405
<210> 220
<211> 403
<212> PRT
<213> 烟曲霉
<400> 220
Met Leu Ser Ile Lys Arg Thr Leu Leu Leu Leu Gly Ala Val Leu Pro
1 5 10 15
Ala Val Phe Gly Ala Pro Val Gln Glu Thr Arg Arg Ala Ala Gln Lys
20 25 30
Ile Pro Gly Lys Tyr Ile Val Thr Phe Lys Pro Gly Thr Asp Thr Ala
35 40 45
Thr Ile Glu Ser His Thr Leu Trp Ala Thr Asp Leu His Lys Arg Asn
50 55 60
Leu Glu Arg Arg Asp Thr Thr Ser Gly Glu Pro Pro Val Gly Ile Glu
65 70 75 80
Lys Ser Tyr Lys Ile Lys Asp Phe Ala Ala Tyr Ala Gly Ser Phe Asp
85 90 95
Asp Ala Thr Ile Glu Glu Ile Arg Lys Arg Gly Asp Val Ala His Val
100 105 110
Glu Glu Asp Gln Ile Trp Tyr Leu Asp Ala Leu Thr Thr Gln Lys Gly
115 120 125
Ala Pro Trp Gly Leu Gly Ser Ile Ser His Lys Gly Gln Ala Ser Thr
130 135 140
Asp Tyr Ile Tyr Asp Thr Ser Ala Gly Ala Gly Thr Tyr Ala Tyr Val
145 150 155 160
Val Asp Ser Gly Ile Asn Val Asn His Val Glu Phe Glu Ser Arg Ala
165 170 175
Ser Leu Ala Tyr Asn Ala Ala Gly Gly Ser His Val Asp Ser Ile Gly
180 185 190
His Gly Thr His Val Ala Gly Thr Ile Gly Gly Lys Thr Tyr Gly Val
195 200 205
Ala Lys Lys Thr Asn Leu Leu Ser Val Lys Val Phe Gln Gly Glu Ser
210 215 220
Ser Ser Thr Ser Ile Ile Leu Asp Gly Phe Asn Trp Ala Val Asn Asp
225 230 235 240
Ile Val Ser Lys Gly Arg Thr Lys Lys Ala Ala Ile Asn Met Ser Leu
245 250 255
Gly Gly Gly Tyr Ser Tyr Ala Phe Asn Asn Ala Val Glu Asn Ala Phe
260 265 270
Asp Glu Gly Val Leu Ser Val Val Ala Ala Gly Asn Glu Asn Ser Asp
275 280 285
Ala Ser Asn Thr Ser Pro Ala Ser Ala Pro Asn Ala Leu Thr Val Ala
290 295 300
Ala Ile Asn Lys Ser Asn Ala Arg Ala Ser Phe Ser Asn Tyr Gly Ser
305 310 315 320
Val Val Asp Ile Phe Ala Pro Gly Gln Asp Ile Leu Ser Ala Trp Ile
325 330 335
Gly Ser Thr Thr Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Thr
340 345 350
Pro His Ile Val Gly Leu Ser Val Tyr Leu Met Gly Leu Glu Asn Leu
355 360 365
Ser Gly Pro Ala Ala Val Thr Ala Arg Ile Lys Glu Leu Ala Thr Asn
370 375 380
Gly Val Val Thr Asn Val Lys Gly Ser Pro Asn Lys Leu Ala Tyr Asn
385 390 395 400
Gly Asn Ala
<210> 221
<211> 403
<212> PRT
<213> 土曲霉
<400> 221
Met Gln Ser Ile Lys Arg Thr Leu Leu Leu Leu Gly Ala Val Leu Pro
1 5 10 15
Ala Val Leu Ala Gly Pro Ile Phe Pro His Arg Arg Ala Pro Thr Thr
20 25 30
Ile Pro Gly Lys Tyr Ile Val Thr Phe Lys Ser Asp Val Asp Gln Ala
35 40 45
Ala Ile Asp Lys His Thr Ala Trp Ala Thr Asp Ile His Lys Arg Asn
50 55 60
Leu Gln Arg Arg Asp Ser Ser Glu Glu Asp Leu Pro Ile Gly Ile Glu
65 70 75 80
Arg Asn Phe Lys Ile Asn Lys Phe Ala Ala Tyr Ser Gly Ser Phe Asp
85 90 95
Glu Asp Thr Ile Ala Gln Ile Arg Gln Ser Asp Glu Val Ala Ala Val
100 105 110
Glu Glu Asp Gln Val Trp His Leu Phe Asp Leu Thr Thr Gln Ser Asp
115 120 125
Ala Pro Trp Gly Leu Gly Ser Ile Ser His Lys Gly Gln Pro Ser Thr
130 135 140
Asp Tyr Ile Tyr Asp Thr Asn Gly Gly Glu Gly Thr Tyr Ala Tyr Val
145 150 155 160
Val Asp Thr Gly Ile Asn Val Asp His Glu Glu Phe Glu Gly Arg Ala
165 170 175
Ser Leu Ala Tyr Asn Ala Ala Gly Gly Gln His Val Asp Gly Val Gly
180 185 190
His Gly Thr His Val Ser Gly Thr Ile Gly Gly Lys Thr Tyr Gly Val
195 200 205
Ala Lys Lys Ala Asn Leu Leu Ser Val Lys Val Phe Val Gly Glu Ser
210 215 220
Ser Ser Thr Ser Ile Ile Leu Asp Gly Phe Asn Trp Ala Ala Asn Asp
225 230 235 240
Ile Val Ser Lys Lys Arg Thr Gly Lys Ala Ala Ile Asn Met Ser Leu
245 250 255
Gly Gly Gly Tyr Ser Lys Ala Phe Asn Asp Ala Val Glu Asn Ala Phe
260 265 270
Asn Glu Gly Val Leu Ser Ile Val Ala Ala Gly Asn Glu Asn Thr Asp
275 280 285
Ala Ser Ser Thr Ser Pro Ala Ser Ala Pro Asp Ala Phe Thr Val Ala
290 295 300
Ala Ile Asn Val Asn Asn Thr Arg Ala Tyr Phe Ser Asn Tyr Gly Ser
305 310 315 320
Val Val Asp Ile Phe Ala Pro Gly Gln Asn Ile Leu Ser Ala Trp Ile
325 330 335
Gly Ser Asn Thr Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Thr
340 345 350
Pro His Ile Val Gly Leu Ser Ile Tyr Leu Met Ser Leu Glu Asp Leu
355 360 365
Ser Ser Pro Lys Ala Val Ser Asp Arg Ile Lys Glu Leu Ala Thr Arg
370 375 380
Gly Val Val Ser Asn Val Ala Gly Ser Pro Asn Leu Leu Ala Tyr Asn
385 390 395 400
Gly Asn Ala
<210> 222
<211> 403
<212> PRT
<213> 米曲霉
<400> 222
Met Gln Ser Ile Lys Arg Thr Leu Leu Leu Leu Gly Ala Ile Leu Pro
1 5 10 15
Ala Val Leu Gly Ala Pro Val Gln Glu Thr Arg Arg Ala Ala Glu Lys
20 25 30
Leu Pro Gly Lys Tyr Ile Val Thr Phe Lys Pro Gly Ile Asp Glu Ala
35 40 45
Lys Ile Gln Glu His Thr Thr Trp Ala Thr Asn Ile His Gln Arg Ser
50 55 60
Leu Glu Arg Arg Gly Ala Thr Gly Gly Asp Leu Pro Val Gly Ile Glu
65 70 75 80
Arg Asn Tyr Lys Ile Asn Lys Phe Ala Ala Tyr Ala Gly Ser Phe Asp
85 90 95
Asp Ala Thr Ile Glu Glu Ile Arg Lys Asn Glu Asp Val Ala Tyr Val
100 105 110
Glu Glu Asp Gln Ile Tyr Tyr Leu Asp Gly Leu Thr Thr Gln Lys Ser
115 120 125
Ala Pro Trp Gly Leu Gly Ser Ile Ser His Lys Gly Gln Gln Ser Thr
130 135 140
Asp Tyr Ile Tyr Asp Thr Ser Ala Gly Glu Gly Thr Tyr Ala Tyr Val
145 150 155 160
Val Asp Ser Gly Val Asn Val Asp His Glu Glu Phe Glu Gly Arg Ala
165 170 175
Ser Lys Ala Tyr Asn Ala Ala Gly Gly Gln His Val Asp Ser Ile Gly
180 185 190
His Gly Thr His Val Ser Gly Thr Ile Ala Gly Lys Thr Tyr Gly Ile
195 200 205
Ala Lys Lys Ala Ser Ile Leu Ser Val Lys Val Phe Gln Gly Glu Ser
210 215 220
Ser Ser Thr Ser Val Ile Leu Asp Gly Phe Asn Trp Ala Ala Asn Asp
225 230 235 240
Ile Val Ser Lys Lys Arg Thr Ser Lys Ala Ala Ile Asn Met Ser Leu
245 250 255
Gly Gly Gly Tyr Ser Lys Ala Phe Asn Asp Ala Val Glu Asn Ala Phe
260 265 270
Glu Gln Gly Val Leu Ser Val Val Ala Ala Gly Asn Glu Asn Ser Asp
275 280 285
Ala Gly Gln Thr Ser Pro Ala Ser Ala Pro Asp Ala Ile Thr Val Ala
290 295 300
Ala Ile Gln Lys Ser Asn Asn Arg Ala Ser Phe Ser Asn Phe Gly Lys
305 310 315 320
Val Val Asp Val Phe Ala Pro Gly Gln Asp Ile Leu Ser Ala Trp Ile
325 330 335
Gly Ser Ser Ser Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Thr
340 345 350
Pro His Ile Val Gly Leu Ser Leu Tyr Leu Ala Ala Leu Glu Asn Leu
355 360 365
Asp Gly Pro Ala Ala Val Thr Lys Arg Ile Lys Glu Leu Ala Thr Lys
370 375 380
Asp Val Val Lys Asp Val Lys Gly Ser Pro Asn Leu Leu Ala Tyr Asn
385 390 395 400
Gly Asn Ala
<210> 223
<211> 416
<212> PRT
<213> 黑曲霉
<400> 223
Met Ala Phe Leu Lys Arg Ile Leu Pro Leu Leu Ala Leu Ile Leu Pro
1 5 10 15
Ala Val Phe Ser Ala Thr Glu Gln Val Pro His Pro Thr Ile Gln Thr
20 25 30
Ile Pro Gly Lys Tyr Ile Val Thr Phe Lys Ser Gly Ile Asp Asn Ala
35 40 45
Lys Ile Glu Ser His Ala Ala Trp Val Thr Glu Leu His Arg Arg Ser
50 55 60
Leu Glu Gly Arg Ser Thr Thr Glu Asp Asp Leu Pro Ala Gly Ile Glu
65 70 75 80
Arg Thr Tyr Arg Ile Ala Asn Phe Ala Gly Tyr Ala Gly Ser Phe Asp
85 90 95
Glu Lys Thr Ile Glu Glu Ile Arg Lys His Asn His Val Ala Tyr Val
100 105 110
Glu Gln Asp Gln Val Trp Tyr Leu Asp Thr Leu Val Thr Glu Arg Arg
115 120 125
Ala Pro Trp Gly Leu Gly Ser Ile Ser His Arg Gly Ala Ser Ser Thr
130 135 140
Asp Tyr Ile Tyr Asp Asp Ser Ala Gly Glu Gly Thr Tyr Ala Tyr Val
145 150 155 160
Val Asp Thr Gly Ile Leu Ala Thr His Asn Glu Phe Gly Gly Arg Ala
165 170 175
Ser Leu Ala Tyr Asn Ala Ala Gly Gly Glu His Val Asp Gly Val Gly
180 185 190
His Gly Thr His Val Ala Gly Thr Ile Gly Gly Lys Thr Tyr Gly Val
195 200 205
Ser Lys Asn Ala His Leu Leu Ser Val Lys Val Phe Val Gly Glu Ser
210 215 220
Ser Ser Thr Ser Val Ile Leu Asp Gly Phe Asn Trp Ala Ala Asn Asp
225 230 235 240
Ile Val Ser Lys Asn Arg Thr Ser Lys Ala Ala Ile Asn Met Ser Leu
245 250 255
Gly Gly Gly Tyr Ser Tyr Ala Phe Asn Asn Ala Val Glu Asn Ala Phe
260 265 270
Asp Glu Gly Val Leu Ser Cys Val Ala Ala Gly Asn Glu Asn Arg Asp
275 280 285
Ala Ala Arg Thr Ser Pro Ala Ser Ala Pro Asp Ala Ile Thr Val Ala
290 295 300
Ala Ile Asn Arg Ser Asn Ala Arg Ala Ser Phe Ser Asn Tyr Gly Ser
305 310 315 320
Val Val Asp Ile Phe Ala Pro Gly Glu Gln Val Leu Ser Ala Trp Thr
325 330 335
Gly Ser Asn Ser Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Thr
340 345 350
Pro His Val Thr Gly Leu Ile Leu Tyr Leu Met Gly Leu Arg Asp Leu
355 360 365
Ala Thr Pro Ala Ala Ala Thr Thr Glu Leu Lys Arg Leu Ala Thr Arg
370 375 380
Asn Ala Val Thr Asn Val Ala Gly Ser Pro Asn Leu Leu Ala Tyr Asn
385 390 395 400
Gly Asn Ser Gly Val Ser Lys Gly Gly Ser Asp Asp Gly Asp Glu Asp
405 410 415
<210> 224
<211> 411
<212> PRT
<213> Glomerella graminicola
<400> 224
Met Ala Asn Leu Arg Arg Leu Ala Leu Ala Ile Ala Ala Leu Leu Pro
1 5 10 15
Ala Val Leu Ala Ala Pro Ala Ala Leu Asn Arg Arg Asp Glu Pro Ala
20 25 30
Ala Ala Pro Gly Lys Tyr Ile Ile Thr Leu Lys Glu Asp Ala Ala Val
35 40 45
Asn Val Asp Ser His Leu Asn Trp Val Ala Asp Val His Arg Arg Ser
50 55 60
Leu Gly Lys Arg Asn Thr Ala Gly Val Glu Asn Lys Phe Asn Ile Ser
65 70 75 80
Thr Trp Asn Ala Tyr Ser Gly Glu Phe Asp Glu Ala Thr Ile Glu Ala
85 90 95
Ile Lys Ala Ser Pro Glu Val Ala Val Val Glu Pro Asp Tyr Tyr Met
100 105 110
Tyr Leu Ser Asp Phe Glu Val Asp Glu Arg Ala Leu Thr Thr Gln Thr
115 120 125
Gly Ala Pro Trp Gly Leu Gly Ser Ile Ser His Lys Thr Lys Gly Ser
130 135 140
Thr Gln Tyr Val Tyr Asp Thr Ser Ala Gly Ala Asn Thr Tyr Ala Tyr
145 150 155 160
Val Val Asp Ser Gly Val Ile Ser Thr His Thr Gln Phe Gly Gly Arg
165 170 175
Val Thr Leu Gly Phe Asn Ala Tyr Ser Gly Val His Thr Asp Thr Leu
180 185 190
Gly His Gly Thr His Val Ala Gly Thr Ile Gly Gly Ser Thr Tyr Gly
195 200 205
Val Ala Lys Gln Thr Asn Ile Ile Ser Val Lys Val Phe Gln Gly Ala
210 215 220
Ser Gly Thr Thr Ser Ser Ile Leu Ser Gly Phe Asn Trp Ala Val Asn
225 230 235 240
Asp Ile Thr Ser Lys Gly Arg Ala Ala Arg Ser Val Ile Asn Met Ser
245 250 255
Leu Gly Gly Pro Ser Ser Thr Thr Trp Asn Thr Ala Ile Asn Ala Ala
260 265 270
Tyr Ser Gln Gly Val Leu Ser Val Val Ala Ala Gly Asn Gly Asp Ser
275 280 285
Leu Gly Asn Pro Leu Pro Val Ser Ser Gln Ser Pro Ala Asn Val Pro
290 295 300
Asn Ala Leu Thr Val Ala Ala Ile Asp Ser Asn Trp Ala Pro Ala Tyr
305 310 315 320
Phe Thr Asn Tyr Gly Pro Gly Val Asp Ile Phe Ala Pro Gly Val Ser
325 330 335
Ile Leu Ser Ala Trp Ile Gly Gly Asn Ser Ala Thr Asn Thr Ile Ser
340 345 350
Gly Thr Ser Met Ala Thr Pro His Val Thr Gly Leu Ala Leu Tyr Leu
355 360 365
Gln Ala Leu Glu Asn Ile Ala Thr Pro Ala Ala Leu Thr Ala Arg Ile
370 375 380
Lys Ala Leu Gly Thr Ser Gly Arg Ile Thr Gly Thr Leu Ser Gly Ser
385 390 395 400
Pro Asn Leu Val Ala Tyr Asn Gly Asn Gly Ala
405 410
<210> 225
<211> 411
<212> PRT
<213> 玉米赤霉
<400> 225
Met Thr Ser Phe Arg Arg Leu Ala Leu Ala Leu Gly Ala Leu Leu Pro
1 5 10 15
Ala Val Leu Ala Ala Pro Thr Glu Lys Arg Gln Glu Leu Thr Ala Ala
20 25 30
Pro Asp Lys Tyr Ile Ile Thr Leu Lys Pro Glu Ala Thr Glu Asn Lys
35 40 45
Ile Glu Ala His Leu Asn Trp Val Ser Asp Val His Arg Arg Ser Leu
50 55 60
Asn Lys Arg Asp Thr Ser Gly Val Glu Lys Lys Phe Asn Ile Ser Ser
65 70 75 80
Trp Asn Ala Tyr Ser Gly Glu Phe Asp Lys Ala Thr Ile Asp Glu Ile
85 90 95
Lys Lys Ser Pro Glu Val Ala Phe Val Glu Pro Asp Tyr Thr Val Tyr
100 105 110
Leu Asp Phe Glu Thr Glu Leu Thr Asp Arg Ala Leu Thr Thr Gln Ser
115 120 125
Gly Ala Pro Trp Gly Leu Ala Ser Ile Ser Arg Arg Thr Ser Gly Gly
130 135 140
Ser Thr Tyr Thr Tyr Asp Thr Thr Ala Gly Ser Gly Ser Tyr Gly Tyr
145 150 155 160
Val Val Asp Ser Gly Ile Asn Val Asn His Arg Asp Phe Gly Gly Arg
165 170 175
Ala Ser Leu Gly Tyr Asn Ala Ala Gly Gly Ala His Val Asp Thr Leu
180 185 190
Gly His Gly Thr His Val Ala Gly Thr Ile Ala Ser Ser Thr Tyr Gly
195 200 205
Val Ala Lys Ala Ala Asn Val Ile Ser Val Lys Val Phe Thr Gly Asn
210 215 220
Ser Ala Ser Thr Ser Thr Ile Leu Ala Gly Phe Asn Trp Ala Val Asn
225 230 235 240
Asp Ile Thr Ser Lys Gly Arg Ala Gly Arg Ser Val Ile Asn Met Ser
245 250 255
Leu Gly Gly Pro Ser Ala Gln Thr Trp Thr Thr Ala Ile Asn Ala Ala
260 265 270
Tyr Asn Ser Gly Val Leu Ser Val Val Ala Ala Gly Asn Gly Asp Asp
275 280 285
Leu Gly Arg Pro Leu Pro Val Ser Gly Gln Ser Pro Ala Asn Val Pro
290 295 300
Asn Ala Leu Thr Val Ala Ala Ile Asp Ser Ser Trp Arg Thr Ala Ser
305 310 315 320
Phe Thr Asn Tyr Gly Ala Gly Val Asp Val Phe Ala Pro Gly Val Gly
325 330 335
Ile Leu Ser Thr Trp Tyr Thr Ser Asn Thr Ala Thr Asn Ser Ile Ser
340 345 350
Gly Thr Ser Met Ala Cys Pro His Val Ala Gly Leu Ala Leu Tyr Leu
355 360 365
Gln Val Leu Glu Gly Leu Ser Thr Pro Ala Ala Val Thr Asn Arg Ile
370 375 380
Lys Ala Leu Ala Thr Thr Gly Arg Val Thr Gly Thr Leu Asn Gly Ser
385 390 395 400
Pro Asn Leu Ile Ala Phe Asn Gly Ala Ser Thr
405 410
<210> 226
<211> 414
<212> PRT
<213> 尖孢镰刀菌
<400> 226
Met Thr Ser Ile Arg Arg Leu Ala Leu Ala Leu Gly Ala Leu Leu Pro
1 5 10 15
Ala Val Leu Ala Ala Pro Ala Asp Ile Leu Ser Lys Arg Gln Ala Val
20 25 30
Pro Asp Lys Tyr Ile Ile Thr Leu Lys Pro Asp Ala Ser Asp Ser Ser
35 40 45
Val Ala Ala His Leu Asn Trp Val Gly Asp Val His Arg Arg Ser Leu
50 55 60
Asn Lys Arg Asp Thr Ser Gly Val Glu Lys Thr Phe Asn Ile Ser Ser
65 70 75 80
Trp Ser Ala Tyr Ser Gly Glu Phe Asp Lys Ser Thr Ile Ala Glu Ile
85 90 95
Lys Lys Ser Pro Glu Val Ala Phe Val Glu Pro Asp Tyr Thr Met Tyr
100 105 110
Leu Ser Tyr Glu Glu Ser Glu Pro Glu Leu Ala Asp Arg Ala Leu Thr
115 120 125
Thr Gln Ser Gly Ala Pro Trp Gly Leu Gly Thr Ile Ser His Arg Thr
130 135 140
Ser Gly Ser Thr Ser Tyr Ile Tyr Asp Thr Thr Ala Gly Gln Gly Ser
145 150 155 160
Tyr Ala Tyr Val Val Asp Ser Gly Val Gln Val Ser His Thr Asn Phe
165 170 175
Gly Gly Arg Ala Ser Leu Gly Tyr Asn Ala Val Gly Gly Ala His Glu
180 185 190
Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile Ala Gly Thr
195 200 205
Thr Tyr Gly Val Ala Lys Arg Ala Asn Ile Ile Ser Val Lys Val Phe
210 215 220
Ala Gly Arg Glu Gly Ser Thr Ser Thr Ile Leu Ala Gly Phe Asn Trp
225 230 235 240
Ala Val Asn Asp Ile Thr Ser Lys Ser Arg Ala Gly Arg Ser Val Ile
245 250 255
Asn Leu Ser Leu Gly Gly Pro Ala Ser Gln Thr Trp Thr Ser Ala Ile
260 265 270
Asn Ala Ala Tyr Asn Ser Gly Val Leu Ser Val Val Ala Ala Gly Asn
275 280 285
Gly Asp Asp Ala Gly Arg Pro Leu Pro Val Ser Gly Gln Ser Pro Ala
290 295 300
Asn Ala Pro Asn Ala Leu Thr Val Ala Ala Ile Asp Ser Ser Trp Arg
305 310 315 320
Pro Ala Ser Phe Thr Asn Tyr Gly Ala Gly Val Asp Val Phe Gly Pro
325 330 335
Gly Val Asn Ile Leu Ser Thr Trp Ile Gly Ser Asn Ser Ala Thr Asn
340 345 350
Thr Ile Ser Gly Thr Ser Met Ala Cys Pro His Val Ala Gly Leu Ala
355 360 365
Leu Tyr Leu Gln Val Leu Glu Gly Leu Ser Thr Pro Ala Ser Val Thr
370 375 380
Asn Arg Ile Lys Ser Leu Ala Thr Thr Gly Arg Ile Thr Gly Thr Leu
385 390 395 400
Ser Gly Ser Pro Asn Ser Val Ala Tyr Asn Gly Asn Gly Ala
405 410
<210> 227
<211> 415
<212> PRT
<213> Nectria haematococca
<400> 227
Met Ala Ser Ile Arg Arg Leu Ala Leu Phe Leu Gly Ala Leu Leu Pro
1 5 10 15
Ala Ala Leu Ala Ala Pro Val Asp Gly Pro Val Arg Arg Gln Glu Thr
20 25 30
Ala Ile Pro Asn Lys Tyr Ile Ile Thr Leu Lys Pro Glu Ala Ser Asp
35 40 45
Ser Lys Ile Ser Ala His Leu Asp Trp Val Gly Asp Val His Arg Arg
50 55 60
Ser Leu Ser Lys Arg Asp Thr Ala Gly Val Glu Glu Thr Tyr Asn Ile
65 70 75 80
Ser Asn Trp Asn Ala Tyr Ala Gly Glu Phe Asp Glu Ala Thr Ile Glu
85 90 95
Glu Ile Lys Ser Ser Pro Glu Val Ala Phe Val Glu Pro Asp Phe Glu
100 105 110
Ala Tyr Leu Trp Val Glu Glu Ser Ser Glu Leu Ser Asp Arg Ala Leu
115 120 125
Thr Thr Gln Thr Gly Ala Thr Trp Gly Leu Gly Thr Ile Ser His Arg
130 135 140
Thr Ser Gly Ser Thr Ser Tyr Ile Tyr Asp Thr Ser Ala Gly Ser Gly
145 150 155 160
Ser Tyr Ala Tyr Val Val Asp Ser Gly Val Gln Val Gly His Ser Gln
165 170 175
Phe Gly Thr Arg Ala Ser Leu Gly Phe Asn Ala Phe Ser Gly Ala His
180 185 190
Thr Asp Thr Leu Gly His Gly Thr His Val Ala Gly Thr Ile Ala Gly
195 200 205
Ser Thr Tyr Gly Val Ala Lys Gln Ala Asn Ile Ile Ser Val Lys Val
210 215 220
Phe Gln Gly Asn Gln Gly Ser Thr Ser Thr Ile Leu Ser Gly Phe Asn
225 230 235 240
Trp Ala Val Asn Asp Ile Thr Ser Lys Gly Arg Gln Gly Arg Ser Val
245 250 255
Ile Asn Leu Ser Leu Gly Gly Glu Ser Ser Gln Thr Trp Ile Ser Ala
260 265 270
Ile Asn Ala Ala Tyr Asn Ser Gly Val Leu Ser Val Val Ala Ala Gly
275 280 285
Asn Gly Asp Ile Asn Gly Asn Pro Leu Pro Val Ser Ser Gln Ser Pro
290 295 300
Ala Asn Ala Pro Asn Ala Leu Thr Val Gly Ala Ile Asp Ser Ser Trp
305 310 315 320
Arg Pro Ala Ser Phe Thr Asn Tyr Gly Ala Gly Val Asp Ile His Ala
325 330 335
Pro Gly Val Ser Val Leu Ser Ser Tyr Ile Gly Ser Asn Thr Ala Thr
340 345 350
Arg Ser Leu Ser Gly Thr Ser Met Ala Cys Pro His Val Ala Gly Leu
355 360 365
Ala Leu Tyr Leu Gln Val Leu Glu Gly Leu Ser Thr Pro Ala Ala Val
370 375 380
Thr Asn Arg Ile Lys Ala Leu Gly Thr Ser Gly Arg Ile Thr Gly Thr
385 390 395 400
Leu Asn Gly Ser Pro Asn Leu Val Ala Tyr Asn Gly Asn Gly Ala
405 410 415
<210> 228
<211> 403
<212> PRT
<213> 费希新萨托菌
<400> 228
Met Leu Ser Ile Lys Arg Thr Leu Leu Leu Leu Gly Ala Val Leu Pro
1 5 10 15
Ala Val Phe Gly Ala Pro Val Gln Glu Thr Arg Arg Ala Ala Gln Lys
20 25 30
Ile Pro Gly Lys Tyr Ile Val Thr Phe Lys Pro Gly Thr Asp Ala Ala
35 40 45
Thr Ile Glu Ser His Thr Leu Trp Ala Thr Asp Leu His Lys Arg Asn
50 55 60
Leu Glu Arg Arg Asp Ala Thr Ser Gly Glu Pro Pro Ile Gly Ile Glu
65 70 75 80
Lys Asn Tyr Lys Ile Lys Asp Phe Ala Ala Tyr Ala Gly Ser Phe Asp
85 90 95
Asp Thr Thr Ile Glu Glu Ile Arg Lys Ser Ala Asp Val Ala His Val
100 105 110
Glu Glu Asp Gln Ile Trp Tyr Ile Asp Ala Leu Thr Thr Gln Lys Gly
115 120 125
Ala Pro Trp Gly Leu Gly Ser Ile Ser His Lys Gly Gln Ala Ser Thr
130 135 140
Asp Tyr Ile Tyr Asp Thr Ser Ala Gly Ala Gly Thr Tyr Ala Tyr Val
145 150 155 160
Val Asp Thr Gly Ile Asn Val Asn His Val Glu Phe Glu Gly Arg Ala
165 170 175
Ser Leu Ala Tyr Asn Ala Ala Gly Gly Ser His Val Asp Ser Val Gly
180 185 190
His Gly Thr His Val Ala Gly Thr Ile Gly Gly Lys Thr Tyr Gly Val
195 200 205
Ala Lys Lys Thr Asn Leu Leu Ser Val Lys Val Phe Gln Gly Glu Ser
210 215 220
Ser Ser Thr Ser Ile Ile Leu Asp Gly Phe Asn Trp Ala Ala Asn Asp
225 230 235 240
Ile Val Ser Lys Gly Arg Thr Arg Lys Ala Ala Ile Asn Met Ser Leu
245 250 255
Gly Gly Gly Tyr Ser Tyr Ala Phe Asn Asn Ala Val Glu Asn Ala Phe
260 265 270
Asp Glu Gly Val Leu Ser Val Val Ala Ala Gly Asn Glu Asn Thr Asp
275 280 285
Ala Ser Asn Thr Ser Pro Ala Ser Ala Pro Asn Ala Leu Thr Val Ala
290 295 300
Ala Ile Asn Arg Ser Asn Ala Arg Ala Ser Phe Ser Asn Tyr Gly Ser
305 310 315 320
Val Val Asp Ile Phe Ala Pro Gly Gln Asp Ile Leu Ser Ala Trp Ile
325 330 335
Gly Ser Asn Thr Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Thr
340 345 350
Pro His Ile Val Gly Leu Ser Val Tyr Leu Met Gly Leu Glu Ser Leu
355 360 365
Ser Gly Pro Ala Ala Val Thr Ser Arg Ile Lys Gln Leu Ala Thr Asn
370 375 380
Gly Val Val Thr Asn Ala Gln Gly Ser Pro Asn Lys Leu Ala Tyr Asn
385 390 395 400
Gly Asn Ala
<210> 229
<211> 393
<212> PRT
<213> 黑白轮枝菌
<400> 229
Met Val Asn Phe Arg Asn Val Leu Leu Gly Leu Leu Pro Phe Ala Ala
1 5 10 15
Ala Ala Pro Val Ala Pro Gly Ser Pro Asp Val Ala Asn Lys Tyr Ile
20 25 30
Val Thr Leu Lys Ser Gly Ile Ser Thr Asp Asn Val Ala Ser His Leu
35 40 45
Asn Trp Val Ser Asp Val His Lys Arg Ser Leu Gly Arg Arg Asp Leu
50 55 60
Ala Gly Val Glu Lys Thr Tyr Asp Ile Lys Asn Phe His Ala Tyr Ala
65 70 75 80
Gly Thr Phe Asp Glu Ser Val Val Ala Glu Leu Lys Asn Asn Pro Asp
85 90 95
Val Ala Ala Val Glu Pro Asp Gln Ile Tyr Thr Leu Ser Ala Tyr Thr
100 105 110
Thr Gln Ser Ser Ala Pro Trp Gly Leu Ala Ser Ile Ser Ser Arg Thr
115 120 125
Ser Gly Ala Thr Ser Tyr Arg Tyr Asp Asn Ser Ala Gly Ala Gly Thr
130 135 140
Phe Ala Tyr Val Val Asp Ser Gly Ile Leu Thr Thr His Thr Asn Phe
145 150 155 160
Gly Gly Arg Ala Ile Arg Gly Tyr Asn Ala Ala Gly Gly Glu Trp Val
165 170 175
Asp Ser Ile Gly His Gly Thr His Val Ala Gly Thr Ile Gly Ser Thr
180 185 190
Thr Tyr Gly Val Ala Lys Ala Ala Thr Leu Ile Asp Val Lys Val Phe
195 200 205
Val Gly Arg Thr Ser Ser Thr Ser Ile Ile Leu Asp Gly Phe Asn Trp
210 215 220
Ala Val Asn Asp Ile Val Ser Lys Asn Arg Ala Ser Arg Ser Val Ile
225 230 235 240
Asn Leu Ser Leu Gly Gly Pro Thr Ser Thr Ala Trp Thr Ser Ala Ile
245 250 255
Asn Ala Ala Tyr Ser Ser Gly Ile Leu Ser Val Ala Ala Ala Gly Asn
260 265 270
Glu Asn Val Ser Ala Ser Thr Arg Ser Pro Ala Asn Ala Pro Asn Ala
275 280 285
Leu Thr Val Gly Ala Ile Asn Ser Ala Trp Ala Glu Asp Thr Ser Tyr
290 295 300
Ser Asn Phe Gly Pro Ala Ile Asp Ile Leu Ala Pro Gly Thr Asn Val
305 310 315 320
Leu Ser Leu Gly Tyr Thr Ser Asn Thr Ser Thr Arg Thr Leu Thr Gly
325 330 335
Thr Ser Met Ala Ala Pro His Val Ala Gly Leu Ala Leu Tyr Leu Ala
340 345 350
Ala Phe Glu Asn Ile Asn Thr Pro Ala Ala Leu Arg Asn Arg Ile Val
355 360 365
Ala Leu Gly Thr Ser Gly Arg Ala Thr Gly Ile Arg Gly Gly Ser Pro
370 375 380
Asn Leu Ile Ala Tyr Asn Gly Asn Ala
385 390
<210> 230
<211> 401
<212> PRT
<213> Glomerella graminicola
<400> 230
Met Val Gly Phe Lys Arg Phe Ala Met Leu Ala Ala Ala Ala Cys Ala
1 5 10 15
Met Ala Ala Pro Thr Arg Arg Glu Glu Ala Thr Glu Val Thr Ala Gln
20 25 30
Thr Ile Glu Gly Ser Tyr Ile Ile Thr Leu Lys Asn Thr Val Ser Val
35 40 45
Glu Gly Leu Thr Ser His Leu Ser Trp Ile Asn Asp Val His Ser Arg
50 55 60
Ser Leu Asp Lys Arg Gln Phe Pro Gly Ala Glu Arg Thr Phe Ser Phe
65 70 75 80
Asn Ser Phe Gln Ala Tyr Ser Gly Lys Phe Asp Asp Ala Thr Ile Asp
85 90 95
Ile Ile Lys Ser Ser Pro Glu Val Ala Ser Val Glu Arg Asp Gln Leu
100 105 110
Trp Thr Leu Ser Ala Ile Ala Thr Gln Thr Gly Ala Pro Trp Gly Leu
115 120 125
Gly Thr Val Ser Arg Arg Thr Ser Gly Ser Thr Gln Tyr Leu Tyr Asp
130 135 140
Thr Asn Ala Gly Ser Gly Thr Phe Ala Tyr Val Val Asp Ser Gly Ile
145 150 155 160
Asn Val Gly His Thr Asn Phe Gly Gly Arg Ala Thr Leu Gly Tyr Asn
165 170 175
Ala Ala Gly Gly Ala His Thr Asp Thr Leu Gly His Gly Thr His Val
180 185 190
Ala Gly Thr Ile Gly Ser Thr Thr Tyr Gly Val Ala Lys Leu Thr Ser
195 200 205
Leu Ile Ser Val Lys Val Phe Val Gly Asn Ser Ala Ser Thr Ser Val
210 215 220
Ile Leu Ala Gly Tyr Asn Trp Ala Val Asn Asp Ile Thr Thr Lys Gly
225 230 235 240
Arg Ala Ser Lys Ser Val Ile Asn Met Ser Leu Gly Gly Pro Ser Ser
245 250 255
Thr Ala Trp Thr Thr Ala Ile Asn Ala Ala Tyr Ser Ala Gly Val Ile
260 265 270
Thr Val Val Ala Ala Gly Asn Glu Ala Gln Asn Ala Ala Asn Val Ser
275 280 285
Pro Ala Asn Ala Pro Asn Ala Ile Thr Val Gly Ala Ile Thr Ser Thr
290 295 300
Trp Ala Ile Ala Ser Tyr Ser Asn Tyr Gly Thr Ser Leu Asp Leu Phe
305 310 315 320
Ala Pro Gly Ser Ser Ile Leu Ser Thr Trp Ile Gly Ser Thr Thr Ala
325 330 335
Thr Asn Ser Ile Ser Gly Thr Ser Met Ala Ser Pro His Val Ala Gly
340 345 350
Leu Val Asn Tyr Leu Gln Ser Val Glu Asn Val Gly Thr Pro Ala Ala
355 360 365
Val Val Ala Arg Ile Lys Ala Leu Ala Thr Thr Gly Arg Val Thr Gly
370 375 380
Asn Leu Arg Ser Ser Pro Asn Leu Ile Ala Tyr Asn Gly Ala Gly Gly
385 390 395 400
Ala
<210> 231
<211> 555
<212> PRT
<213> 里氏木霉
<400> 231
Met Arg Ala Cys Leu Leu Phe Leu Gly Ile Thr Ala Leu Ala Thr Ala
1 5 10 15
Ile Pro Ala Leu Lys Pro Pro His Gly Ser Pro Asp Arg Ala His Thr
20 25 30
Thr Gln Leu Ala Lys Val Ser Ile Ala Leu Gln Pro Glu Cys Arg Glu
35 40 45
Leu Leu Glu Gln Ala Leu His His Leu Ser Asp Pro Ser Ser Pro Arg
50 55 60
Tyr Gly Arg Tyr Leu Gly Arg Glu Glu Ala Lys Ala Leu Leu Arg Pro
65 70 75 80
Arg Arg Glu Ala Thr Ala Ala Val Lys Arg Trp Leu Ala Arg Ala Gly
85 90 95
Val Pro Ala His Asp Val Leu Thr Asp Gly Gln Phe Ile His Val Arg
100 105 110
Thr Leu Ala Glu Lys Ala Gln Ala Leu Leu Gly Phe Glu Tyr Asn Ser
115 120 125
Thr Leu Gly Ser Gln Thr Ile Ala Ile Ser Thr Leu Pro Gly Lys Ile
130 135 140
Arg Lys His Val Met Thr Val Gln Tyr Val Pro Leu Trp Thr Glu Ala
145 150 155 160
Asp Trp Glu Glu Cys Lys Thr Ile Ile Thr Pro Ser Cys Leu Lys Arg
165 170 175
Leu Tyr His Val Asp Ser Tyr Arg Ala Lys Tyr Glu Ser Ser Ser Leu
180 185 190
Phe Gly Ile Val Gly Phe Ser Gly Gln Ala Ala Gln His Asp Glu Leu
195 200 205
Asp Lys Phe Leu His Asp Phe Ala Pro Tyr Ser Thr Asn Ala Asn Phe
210 215 220
Ser Ile Glu Ser Val Asn Gly Gly Gln Ser Pro Gln Gly Met Asn Glu
225 230 235 240
Pro Ala Ser Glu Ala Asn Gly Asp Val Gln Tyr Ala Val Ala Met Gly
245 250 255
Tyr His Val Pro Val Arg Tyr Tyr Ala Val Gly Gly Glu Asn His Asp
260 265 270
Ile Ile Pro Asp Leu Asp Leu Val Asp Thr Thr Glu Glu Tyr Leu Glu
275 280 285
Pro Phe Leu Glu Phe Ala Ser His Leu Leu Asp Leu Asp Asp Asp Glu
290 295 300
Leu Pro Arg Val Val Ser Ile Ser Tyr Gly Ala Asn Glu Gln Leu Phe
305 310 315 320
Pro Arg Ser Tyr Ala His Gln Val Cys Asp Met Phe Gly Gln Leu Gly
325 330 335
Ala Arg Gly Val Ser Ile Val Val Ala Ala Gly Asp Leu Gly Pro Gly
340 345 350
Val Ser Cys Gln Ser Asn Asp Gly Ser Ala Arg Pro Lys Phe Ile Pro
355 360 365
Ser Phe Pro Ala Thr Cys Pro Tyr Val Thr Ser Val Gly Ser Thr Arg
370 375 380
Gly Ile Met Pro Glu Val Ala Ala Ser Phe Ser Ser Gly Gly Phe Ser
385 390 395 400
Asp Tyr Phe Ala Arg Pro Ala Trp Gln Asp Arg Ala Val Gly Ala Tyr
405 410 415
Leu Gly Ala His Gly Glu Glu Trp Glu Gly Phe Tyr Asn Pro Ala Gly
420 425 430
Arg Gly Phe Pro Asp Val Ala Ala Gln Gly Val Asn Phe Arg Phe Arg
435 440 445
Ala His Gly Asn Glu Ser Leu Ser Ser Gly Thr Ser Leu Ser Ser Pro
450 455 460
Val Phe Ala Ala Leu Ile Ala Leu Leu Asn Asp His Arg Ser Lys Ser
465 470 475 480
Gly Met Pro Pro Met Gly Phe Leu Asn Pro Trp Ile Tyr Thr Val Gly
485 490 495
Ser His Ala Phe Thr Asp Ile Ile Glu Ala Arg Ser Glu Gly Cys Pro
500 505 510
Gly Gln Ser Val Glu Tyr Leu Ala Ser Pro Tyr Ile Pro Asn Ala Gly
515 520 525
Trp Ser Ala Val Pro Gly Trp Asp Pro Val Thr Gly Trp Gly Thr Pro
530 535 540
Leu Phe Asp Arg Met Leu Asn Leu Ser Leu Val
545 550 555
<210> 232
<211> 561
<212> PRT
<213> 深绿木霉
<400> 232
Met Lys Ala Ser Arg Leu Leu Phe Phe Gly Val Asn Ala Thr Val Thr
1 5 10 15
Ala Ser Pro Ile Gln Gln Leu Gly Glu Ser Ser Lys Ala Ala Gln Val
20 25 30
Val Lys Leu Ser Ile Ala Leu Gln Pro Glu Ser Arg Gln Leu Leu Glu
35 40 45
Gln Thr Ile Tyr Asn Leu Ser Ser Pro Ser Gly Ser Arg Tyr Gly Gln
50 55 60
Tyr Leu Gly Arg Glu Glu Ala Lys Gly Leu Leu Arg Pro Arg Lys Ser
65 70 75 80
Ser Thr Asp Ala Val Lys Gly Trp Leu Ser Gln Ala Gly Ile Arg Ser
85 90 95
Gly His Ile Leu Ser Asp Gly Gln Phe Ile His Val Gln Thr Asp Val
100 105 110
Gly Ser Ile Glu Ala Leu Phe Glu Val Asn Tyr Asn Ala Thr Leu Gly
115 120 125
Ser Gln Thr Val Ser Val Ser Ser Leu Pro Leu Arg Ile Gln Gly His
130 135 140
Val Met Thr Val Gln Tyr Ala Pro Tyr Leu Thr Arg Val Arg Thr Asp
145 150 155 160
Trp Glu Arg Cys Lys Thr Glu Ile Thr Pro Ala Cys Leu Lys Lys Leu
165 170 175
Tyr His Val Asp Ser Tyr Gln Ala Lys Gly Glu Glu Arg Asn Leu Val
180 185 190
Gly Val Ala Gly Phe Asp Gly Gln Ala Ile Gln Tyr His Glu Leu Gly
195 200 205
Glu Phe Leu His Glu Leu Val Pro Pro Pro Ala Ala Phe Asn Val Ser
210 215 220
Val Ala Phe Val Asn Gly Gly Thr Asn Pro Gln Gly Thr Asn Phe Pro
225 230 235 240
Ser Ser Glu Ala Asn Gln Asp Val Gln Tyr Ala Val Gly Met Ala His
245 250 255
Ser Val Pro Val Arg Phe Tyr Ala Thr Gly Gly Glu Asn His Asp Ile
260 265 270
Ile Pro Asp Leu Asp Leu Thr Asp Pro Thr Asn Glu Tyr Leu Glu Pro
275 280 285
Tyr Leu Glu Phe Ala Asn His Leu Leu Asn Leu Pro Asp Glu Gln Leu
290 295 300
Pro Ser Val Ile Ser Ile Ser Tyr Gly Ala Asn Glu Gln Leu Phe Pro
305 310 315 320
Lys Pro Tyr Ala Gln Gln Val Cys Asp Met Phe Gly Gln Leu Gly Thr
325 330 335
Arg Gly Val Ser Ile Ile Val Ser Ser Gly Asp Phe Gly Pro Gly Met
340 345 350
Ser Cys Gln Ser Asn Asp Gly Thr Glu Thr Thr Lys Tyr Ile Pro Ser
355 360 365
Phe Pro Ala Thr Cys Pro Tyr Val Thr Ser Val Gly Ser Thr Glu Gly
370 375 380
Ile Gly Pro Glu Arg Ala Ala Asn Phe Ser Ala Gly Gly Phe Ser Asp
385 390 395 400
Tyr Phe Ala Arg Pro Glu Trp Gln Asp Glu Ala Val Gly Gly Tyr Leu
405 410 415
Glu Leu Tyr Gly Asn Glu Trp Lys Gly Tyr Tyr Asn Ala Gly Gly Arg
420 425 430
Gly Phe Pro Asp Val Ala Ala Gln Gly Val Asn Tyr Arg Phe Trp Asn
435 440 445
His Gly Lys Glu Asp Leu Thr Thr Gly Thr Ser Val Ser Thr Pro Val
450 455 460
Phe Ala Ala Leu Val Ala Leu Leu Asn Asp His Arg Ala Glu Arg Gly
465 470 475 480
Leu Pro Arg Met Gly Phe Leu Asn Pro Trp Ile Tyr Ser Ile Gly Asn
485 490 495
Gly Ala Phe Thr Asp Ile Thr Gln Ser Lys Ser Ile Gly Cys Gln Gly
500 505 510
Gln Ser Leu Ser Gly Leu Ala Ser Pro Val Ile Pro Asn Ala Gly Trp
515 520 525
Ser Ala Val Lys Gly Trp Asp Pro Val Thr Gly Trp Gly Thr Pro Leu
530 535 540
Phe Asp Lys Met Met Ser Leu Ser Gly Gly Gly Val Asp Glu Glu Ser
545 550 555 560
His
<210> 233
<211> 556
<212> PRT
<213> 绿木霉
<400> 233
Met Lys Ala Arg Leu Leu Phe Leu Ala Phe Asn Ala Leu Ala Met Ala
1 5 10 15
Thr Pro Met Ala Lys Pro Pro Leu Gly Thr Leu Lys Gly Val Gln Pro
20 25 30
Ile Gln Ser Gly Lys Ile Ser Ile Ala Leu Gln Pro Glu Cys Arg Glu
35 40 45
Leu Leu Glu Gln Thr Leu His Tyr Leu Ser Asp Pro Ser Ser Arg Arg
50 55 60
Tyr Gly Gln Tyr Leu Gly Arg Glu Glu Ala Lys Ala Leu Leu Arg Pro
65 70 75 80
Arg Gln Asp Ser Thr Asp Ala Val Lys Gly Trp Leu Ser Gln Ala Gly
85 90 95
Ile Pro Ala Ile Asp Ile Gln Ser Asp Gly Gln Phe Ile Asn Val Gln
100 105 110
Val Met Ala Glu Gln Ala Arg Ala Leu Leu Gly Thr Gly Tyr Asn Ser
115 120 125
Thr Leu Gly Ile Gln Thr Ile Pro Ile Ser Ser Leu Pro Lys Asp Ile
130 135 140
Glu Ser His Val Met Thr Ile His Tyr Ala Pro Thr Arg Thr Glu Met
145 150 155 160
Val Thr Asp Trp Glu Glu Cys Lys Ser Arg Ile Thr Pro Asn Cys Leu
165 170 175
Lys Lys Leu Tyr His Val Asp Gly Tyr Arg Ala Arg His Val Asn Lys
180 185 190
Asn Arg Phe Gly Ile Val Gly Phe Thr Gly Gln Ala Ala Gln Tyr Asp
195 200 205
Gln Leu Ser Ala Phe Leu Asp Asp Phe Ala Pro Tyr Ala Ala Asn Ala
210 215 220
Asn Phe Ser Val Glu Ser Val Asn Gly Gly Glu Asn Pro Gln Gly Arg
225 230 235 240
Asn Glu Pro Thr Gly Glu Ala Asn Val Asp Ile Gln Tyr Ala Val Ser
245 250 255
Met Ala Tyr Asp Val Pro Val Arg Phe Tyr Ala Thr Gly Gly Ala Asn
260 265 270
His Asp Ile Ile Pro Asp Leu Asp Leu Ser Asp Thr Asp Asn Gln Ala
275 280 285
Leu Glu Pro Tyr Leu Glu Phe Ala Ser His Leu Leu Ser Leu Asp Asp
290 295 300
Asp Glu Leu Pro Lys Val Val Ser Ile Ser Tyr Gly Ala Asn Glu Gln
305 310 315 320
Leu Phe Pro Arg Ser Tyr Ala Gln Gln Val Cys Asp Met Phe Gly Gln
325 330 335
Leu Gly Thr Arg Gly Val Ser Ile Val Val Ala Ser Gly Asn Leu Gly
340 345 350
Pro Gly Val Ser Cys Gln Ser Asn Asp Gly Thr Lys Arg Pro Lys Phe
355 360 365
Met Pro Ser Phe Pro Ala Thr Cys Pro Tyr Val Thr Ser Val Gly Ala
370 375 380
Thr Tyr Gly Ile Asn Pro Glu Ile Ala Val Asn Phe Ser Ser Gly Gly
385 390 395 400
Phe Ser Asp Tyr Phe Ile Arg Pro Gln Trp Gln Asp Glu Ala Ile Glu
405 410 415
Gly Tyr Leu Lys Leu His Gly Arg Glu Trp Lys Gly Tyr Tyr Asn Pro
420 425 430
His Gly Arg Gly Phe Pro Asp Val Ala Ala Gln Gly Val Asn Tyr Arg
435 440 445
Phe Trp Ser His Gly Asn Glu Asp Ser Ala Ser Gly Thr Ser Val Ser
450 455 460
Ser Pro Val Phe Ala Ala Leu Ile Ala Leu Leu Asn Asp Asn Arg Thr
465 470 475 480
Lys Asn Gly Leu Pro Ser Met Gly Phe Leu Asn Pro Trp Ile Tyr Ser
485 490 495
Ile Gly Ser His Ala Phe Thr Asp Ile Thr Glu Ser Lys Ser Leu Gly
500 505 510
Cys Ser Gly Arg Ser Val Ile Gly Leu Glu Ser Pro Val Ile Pro Asn
515 520 525
Ala Gly Trp Asp Ala Val Pro Gly Trp Asp Pro Val Thr Gly Trp Gly
530 535 540
Thr Pro Leu Phe Asp Arg Leu Met Asn Leu Ser Tyr
545 550 555
<210> 234
<211> 592
<212> PRT
<213> 金龟子绿僵菌
<400> 234
Met Ser Ile Leu Gly Leu Leu Cys Val Leu Gly Ser Ile Phe Leu Val
1 5 10 15
Val Ser Ala Asp Leu Val Val Val Glu His Leu Asp Gln Pro Pro Asp
20 25 30
Gly Trp Thr Lys Leu Gly Ala Ala Asn Ala Ser Gln Leu Ile Arg Leu
35 40 45
Ser Ile Ala Leu Glu Ser Gln Gly His Asp Val Phe Glu Arg Thr Leu
50 55 60
Gln Glu Val Ser Asp Pro Thr His Pro Arg Tyr Gly Gln Tyr Leu Ser
65 70 75 80
Arg Asp Glu Ala Val Ala Leu Val Arg Pro Arg Pro Arg Ser Val Ala
85 90 95
Ala Val Arg Glu Trp Leu Leu Ser Glu Asn Val Thr Gln Gly Gln Val
100 105 110
Gln Glu Arg Gly Leu Phe Val Asp Ala Phe Met Thr Ile Gly Thr Ala
115 120 125
Glu Asn Leu Leu Ser Thr Thr Tyr Gly Leu Phe Arg His Gly Lys Gln
130 135 140
Arg Ala Ile Gly Ala Leu Ala Tyr Ser Val Pro Ala Glu Val Arg Pro
145 150 155 160
His Ile Thr Ser Ile Gln Pro Thr Thr Phe Phe Glu Phe Gly Asn Leu
165 170 175
Phe Lys Lys Ser Pro Arg Thr Ser Gly Val Gly Ile Ala Thr Ala Arg
180 185 190
Arg Arg Asn Pro Gly Val Arg Ala Arg Ser Thr Glu Leu Gln Asp Cys
195 200 205
Glu Asn His Asn Thr Pro Glu Cys Leu Arg Gly Leu Tyr Lys Met Ser
210 215 220
Asn Asp Tyr Thr Lys Pro His Arg Ser Ser Leu Leu Gly Val Ala Gly
225 230 235 240
Phe His Gly Gln Ala Ala Gln His Asp Gln Leu Glu Lys Tyr Leu Asp
245 250 255
Thr Tyr Ala Pro Tyr Ala Lys Gly Ala Asn Phe Ser Val Glu Leu Ile
260 265 270
Asn Asn Gly Thr Asn Pro Gln Gly Glu Tyr Pro Gly Phe Glu Ala Asn
275 280 285
Met Asp Ile Gln Ile Ala Val Ser Met Ala Phe Arg Val Pro Val Arg
290 295 300
Phe Tyr Ser Thr Gly Gly Glu Gly His Glu Phe Ile Pro Asp Leu Asp
305 310 315 320
Val Tyr Asp Pro Glu Lys Gln His Leu Glu Pro Trp Leu Gln Phe Ala
325 330 335
Ser Tyr Leu Leu Asp Leu Pro Asp Arg Asp Leu Pro Gln Val Met Ser
340 345 350
Ile Ser Tyr Gly Gly Asn Glu Gln Ala Val Pro Lys Pro Tyr Ala Arg
355 360 365
Arg Ile Cys Gln Met Phe Gly Leu Leu Ala Leu Arg Gly Val Ser Ile
370 375 380
Ile Leu Ala Ser Gly Asp Gln Gly Pro Gly Met Ser Cys Gln Ser Asn
385 390 395 400
Asp Gly Thr Asn Ser Thr Lys Phe Leu Pro Ala Phe Pro Ala Gly Cys
405 410 415
Pro Tyr Leu Thr Val Val Gly Ala Thr Glu Arg Asn Ala Pro Glu Arg
420 425 430
Ala Met Asn Phe Ser Ser Gly Gly Phe Ser Glu Tyr Trp Pro Arg Pro
435 440 445
Ala Trp Gln Glu Ala Ala Val Ser Arg Tyr Leu Asp Ala His Gly Asp
450 455 460
Arg Trp Lys Gly Tyr Tyr Asn Arg Ala Gly Arg Gly Phe Pro Asp Val
465 470 475 480
Ser Ala Gln Gly Ile Gly Tyr Pro Phe Phe Asn His Gly Gly Asn Ala
485 490 495
Ser Gly Gly Gly Thr Ser Ala Ser Ala Pro Leu Phe Ala Ser Met Ile
500 505 510
Ala Val Ile Asn Asp Ser Arg Met Lys Arg Gly Lys Pro Pro Leu Gly
515 520 525
Phe Leu Asn Pro Trp Leu Tyr Leu Gly Ala Ser Arg Ala Phe Thr Glu
530 535 540
Ser Glu Gly Cys Lys Gly Thr Ser Phe Ser Gly Ala Lys Ala Pro Val
545 550 555 560
Ile Pro Gly Ala Gly Trp Asp Ala Val Glu Gly Trp Asp Pro Val Thr
565 570 575
Gly Leu Gly Thr Pro Leu Phe Asp Glu Leu Gln Glu Leu Ala Met Gly
580 585 590
<210> 235
<211> 603
<212> PRT
<213> Nectria haematococca
<400> 235
Met Met Ala Ile Ser Leu Leu Lys Thr Trp Gly Phe Leu Leu Leu Thr
1 5 10 15
Ala Thr Ala Val Ala Arg Pro Val Val Val Glu Ser Leu Asp Glu Thr
20 25 30
Pro Glu Gly Trp Glu Glu Ser Ser Ser Pro Ala Pro Asp Lys Pro Ile
35 40 45
Glu Phe Ser Ile Gly Leu Glu Ser Glu Asp His Phe Leu Leu Glu Arg
50 55 60
Thr Leu Ser Glu Ile Ser Asp Pro Ser His Ala Asn Tyr Gly Lys His
65 70 75 80
Leu Ser Arg Asp Ala Ala Lys Ala Leu Leu His Pro Ser Arg Ala Ala
85 90 95
Thr Asp Ser Val Lys Arg Trp Leu Ser Asp Ala Gly Val Pro Glu His
100 105 110
His Val Arg Asp Glu Gly Gln Trp Leu His Ile Arg Thr Thr Val Gly
115 120 125
Gln Ala Asp Ser Leu Leu Ser Thr Arg Phe Gly Ile Phe Ala Arg Asp
130 135 140
Asp Glu Gln Val Val Arg Thr Arg Glu Tyr Ser Val Pro Ile Glu Val
145 150 155 160
Arg Asp His Ile Thr Thr Ile Gln Pro Thr Thr Phe Phe Pro Thr Glu
165 170 175
Lys Lys Ala Arg Ala Val Glu Asp Leu Thr Ala Leu Glu Lys Arg Glu
180 185 190
Glu Ala Val Glu Ala Arg Ala Tyr Gly Asn Asn Asn Asp Gly Asn His
195 200 205
Gly Gly Asn Gly Pro Ile Asp Leu Gln Gln Cys Lys Ala Gln Leu Thr
210 215 220
Pro Ala Cys Leu Arg Lys Ile Tyr Lys Met Pro Leu Arg Ser Tyr Pro
225 230 235 240
Ser Ala His Arg Lys Ser Glu Tyr Gly Ile Val Gly Phe Leu Glu Gln
245 250 255
Asn Ala Gln Phe Ser Asp Leu Glu Glu Phe Leu Lys Arg Phe Ala Pro
260 265 270
Asp Leu Lys Gly Ala Asn Phe Ser Val Ser Leu Val Asn Gly Gly Leu
275 280 285
Asn Gln Gln Ser Phe Asn Val Ala Gln Ile Glu Ala Asn Met Asp Ile
290 295 300
Gln Tyr Ala Val Ala Leu Ala Asp Lys Val Pro Val Arg Phe Ile Ser
305 310 315 320
Val Gly Gly Met Asn Pro Asn Leu Val Pro Asp Leu Glu Gln Tyr Pro
325 330 335
Glu Pro Trp Leu Glu Leu Leu Gln Tyr Leu Leu Asn Leu Pro Asn Ser
340 345 350
Asn Leu Pro Lys Val Ile Ser Leu Ser Tyr Gly Ser Asn Glu Gln His
355 360 365
Ile Ser Lys Ser Tyr Ala Arg Gln Val Cys Asn Met Phe Gly Gln Leu
370 375 380
Gly Thr Arg Gly Val Ser Val Ile Ala Ala Ala Gly Asn Thr Gly Pro
385 390 395 400
Gly Phe Ser Cys Lys Pro Asn Asp Gly Lys Asn Lys Lys Lys Phe Leu
405 410 415
Pro Thr Phe Pro Gly Ala Cys Pro Tyr Val Thr Ser Val Gly Gly Thr
420 425 430
Glu Gly Asn Ser Pro Glu Ile Ala Trp Asn Arg Ser Ser Gly Gly Phe
435 440 445
Ser Glu Val Phe Ser Arg Pro Trp Trp Gln Glu Gln Thr Val Lys Glu
450 455 460
Tyr Leu Arg Lys His Gly Asn Glu Trp Lys Gly Tyr Tyr Asn Ala Asn
465 470 475 480
Gly Arg Ala Tyr Pro Asp Val Ala Ala Leu Ala Trp Asn His Gln Ile
485 490 495
Met Leu Arg Gly Asn Gln Thr Phe Gly Gly Gly Thr Ser Val Ala Ala
500 505 510
Pro Thr Phe Gly Ala Met Ile Ala Leu Ile Asn Asn Glu Arg Phe Lys
515 520 525
Lys Gly Lys Pro Pro Met Gly Phe Leu Asn Pro Trp Leu Tyr Lys Thr
530 535 540
Gly Lys Ala Gly Phe Thr Asp Ile Thr Gln Gly Lys Ser Val Gly Cys
545 550 555 560
Thr Gly Glu Ser Tyr Leu Gly Leu Pro Ser Pro Val Ile Pro Ser Ala
565 570 575
Gly Trp Lys Ala Val Lys Gly Trp Asp Pro Ile Thr Gly Trp Gly Thr
580 585 590
Pro Leu Phe Asp Arg Leu Gln Arg Leu Ala Thr
595 600
<210> 236
<211> 621
<212> PRT
<213> 嗜热毁丝霉
<400> 236
Met Leu Arg Asn Ile Phe Leu Thr Ala Ala Leu Ala Ala Phe Gly Gln
1 5 10 15
Cys Gly Ser Thr Val Phe Glu Ser Val Pro Ala Lys Pro Arg Gly Trp
20 25 30
Thr Arg Leu Gly Asp Ala Ser Ala Asp Gln Pro Leu Arg Leu Arg Ile
35 40 45
Ala Leu Gln Gln Pro Asn Glu Asp Leu Phe Glu Arg Thr Leu Tyr Glu
50 55 60
Val Ser Asp Pro Ser His Ala Arg Tyr Gly Gln His Leu Ser Arg Asp
65 70 75 80
Glu Leu Ser Ala Leu Leu Ala Pro Arg Ala Glu Ser Thr Ala Ala Val
85 90 95
Leu Asn Trp Leu Arg Asp Ala Gly Ile Pro Ser Asp Lys Ile Glu Glu
100 105 110
Asp Gly Glu Trp Ile Asn Leu Arg Val Thr Val Arg Glu Ala Ser Glu
115 120 125
Leu Leu Asp Ala Asp Phe Gly Val Trp Ala Tyr Glu Gly Thr Asn Val
130 135 140
Lys Arg Val Arg Ala Leu Gln Tyr Ser Val Pro Glu Glu Ile Ala Pro
145 150 155 160
His Ile Arg Met Val Ala Pro Val Val Arg Phe Gly Gln Ile Arg Pro
165 170 175
Glu Arg Ser Gln Val Phe Glu Val Val Glu Thr Ala Pro Ser Gln Val
180 185 190
Lys Val Ala Ala Ala Ile Pro Pro Gln Asp Leu Asp Val Lys Ala Cys
195 200 205
Asn Thr Ser Ile Thr Pro Glu Cys Leu Arg Ala Leu Tyr Lys Val Gly
210 215 220
Ser Tyr Gln Ala Glu Pro Ser Lys Lys Ser Leu Phe Gly Val Ala Gly
225 230 235 240
Tyr Leu Glu Gln Trp Ala Lys Tyr Asp Gln Leu Glu Leu Phe Ala Ser
245 250 255
Thr Tyr Ala Pro Tyr Ala Ala Asp Ala Asn Phe Thr Ser Val Gly Val
260 265 270
Asn Gly Gly Glu Asn Asn Gln Gly Pro Ser Asp Gln Gly Asp Ile Glu
275 280 285
Ala Asn Leu Asp Ile Gln Tyr Ala Val Ala Leu Ser Tyr Lys Thr Pro
290 295 300
Ile Thr Tyr Tyr Ile Thr Gly Gly Arg Gly Pro Leu Val Pro Asp Leu
305 310 315 320
Asp Gln Pro Asp Pro Asn Asp Val Ser Asn Glu Pro Tyr Leu Glu Phe
325 330 335
Phe Ser Tyr Leu Leu Lys Leu Pro Asp Ser Glu Leu Pro Gln Thr Leu
340 345 350
Thr Thr Ser Tyr Gly Glu Asp Glu Gln Ser Val Pro Arg Pro Tyr Ala
355 360 365
Glu Lys Val Cys Gln Met Ile Gly Gln Leu Gly Ala Arg Gly Val Ser
370 375 380
Val Ile Phe Ser Ser Gly Asp Thr Gly Val Gly Ser Ala Cys Gln Thr
385 390 395 400
Asn Asp Gly Lys Asn Thr Thr Arg Phe Leu Pro Ile Phe Pro Gly Ala
405 410 415
Cys Pro Tyr Val Thr Ser Ile Gly Ala Thr Arg Tyr Val Glu Pro Glu
420 425 430
Gln Ala Ala Ala Phe Ser Ser Gly Gly Phe Ser Asp Ile Phe Lys Arg
435 440 445
Pro Ala Tyr Gln Glu Ala Ala Val Ser Thr Tyr Leu His Lys His Leu
450 455 460
Gly Ser Arg Trp Lys Gly Leu Tyr Asn Pro Gln Gly Arg Gly Phe Pro
465 470 475 480
Asp Val Ser Ala Gln Gly Val Ala Tyr His Val Phe Ser Gln Asp Lys
485 490 495
Asp Ile Lys Val Ser Gly Thr Ser Ala Ser Ala Pro Leu Phe Ala Ala
500 505 510
Leu Val Ser Leu Leu Asn Asn Ala Arg Leu Ala Gln Gly Arg Pro Pro
515 520 525
Leu Gly Phe Leu Asn Pro Trp Leu Tyr Ser Glu Lys Val Gln Lys Ala
530 535 540
Gly Ala Leu Thr Asp Ile Val His Gly Gly Ser Ser Gly Cys Thr Gly
545 550 555 560
Lys Asp Met Tyr Ser Gly Leu Pro Thr Pro Tyr Val Pro Tyr Ala Ser
565 570 575
Trp Asn Ala Thr Pro Gly Trp Asp Pro Val Thr Gly Leu Gly Thr Pro
580 585 590
Val Phe Asp Lys Leu Leu Glu Leu Ser Ser Pro Gly Lys Lys Leu Pro
595 600 605
His Ile Gly Gly Gly His Gly His Gly Ala Gly Gly His
610 615 620
<210> 237
<211> 613
<212> PRT
<213> 玉米赤霉
<400> 237
Met Leu Ala Ile Asn Leu Leu Lys Thr Gly Gly Phe Leu Phe Ser Ala
1 5 10 15
Ala Ser Val Leu Ala Val Pro Val Val Val Glu Ser Leu Asn Glu Thr
20 25 30
Pro Ala Gly Trp Glu Glu Ser Asp Ser Pro Ser Pro Asp Glu Phe Ile
35 40 45
Asn Leu Ser Ile Gly Leu Glu Pro Glu Asp His Lys Leu Leu Glu Arg
50 55 60
Thr Ile Tyr Glu Val Ser Asp Pro Asp His His Asn Tyr Gly Lys His
65 70 75 80
Leu Ser Arg Glu Ser Ala Lys Ala Leu Leu Arg Pro Ser Ser Ala Ala
85 90 95
Thr Lys Ser Val Glu Arg Trp Leu Ser Glu Ala Gly Val Pro Asp His
100 105 110
His Val Arg Asp Glu Gly Glu Trp Leu His Ile Gln Thr Thr Val Lys
115 120 125
Asn Ala Glu Gly Met Leu Asn Thr Arg Phe Ser Val Phe Ala Arg Asp
130 135 140
Asp Arg Ser Ile Val Arg Thr Arg Glu Tyr Ser Val Pro His Glu Ile
145 150 155 160
Arg Arg His Ile Ser Ser Ile Gln Pro Thr Thr Leu Phe Ser Ser Phe
165 170 175
Gln His Thr Gln Ser Leu Glu Glu Thr Ala Pro Thr Val Met Lys Arg
180 185 190
Tyr Val Gln Gly Glu Lys Glu Pro Arg Ser Thr Lys Asn Gly Asn Tyr
195 200 205
Gly Gly Pro Gly Pro Val Asp Leu Lys Lys Cys Lys Thr Glu Ala Thr
210 215 220
Pro Ala Cys Ile Arg Lys Leu Tyr Asn Leu Pro Lys Lys Tyr Pro Asn
225 230 235 240
Ala Ala Lys Gly Ser Leu Tyr Thr Thr Val Ser Phe Gln Asn Met Thr
245 250 255
Ser Gln Arg Asp Glu Leu Gln Glu Phe Leu Arg Arg Tyr Ala Pro Asp
260 265 270
Leu Lys Gly Val Thr Phe Ala Asp Asp Phe Ala Asn Gly Gly Lys Asn
275 280 285
Thr Gln Gly Asp Asn Val Pro Glu Asn Glu Gly Asn Leu Asn Thr Gln
290 295 300
Tyr Ala Val Ser Leu Ala Ser Arg Val Pro Ile Gln His Leu Ala Val
305 310 315 320
Gly Gly Ile Asn Thr Asp Tyr Ile Ser Asp Leu Asp Leu Pro Pro Gly
325 330 335
Gln Gly Ile Glu Thr Trp Leu Glu Phe Ala Glu His Ile Leu Asn Leu
340 345 350
Pro Thr Lys Lys Leu Pro Ser Val Ile Ser Ile Ser Trp Gly Glu Tyr
355 360 365
Glu Gln His Leu Pro Lys Lys Tyr Ala Arg Gln Val Cys Asn Lys Phe
370 375 380
Gly Gln Ile Gly Thr Arg Gly Val Ser Ile Ile Val Pro Ala Gly Asn
385 390 395 400
Gln Gly Val Gly Val Ala Cys Gln Ser Asn Asp Gly Lys Lys Thr Lys
405 410 415
Lys Phe Leu Pro Thr Trp Pro Ser Ala Cys Pro Tyr Val Thr Thr Val
420 425 430
Gly Gly Thr Gly Gly Asn Asn Pro Glu Ile Ala Leu Ser Asp Gly Glu
435 440 445
Gly Glu Pro Phe Ser Thr Gly Gly Gly Phe Ser Asp Leu Phe Pro Arg
450 455 460
Pro Glu Tyr Gln Glu Lys Ala Val Lys Glu Tyr Leu Lys Lys Tyr Gly
465 470 475 480
Lys Lys Trp Gln Ala Tyr Tyr Asn Pro Lys Gly Arg Ala Tyr Pro Asp
485 490 495
Val Ala Ala Ile Ala Ala Glu Leu Pro Val Met Val Arg Gly Glu Val
500 505 510
Arg Pro Ala Thr Gly Thr Ser Ala Ala Ala Pro Thr Phe Gly Ala Ile
515 520 525
Val Ala Leu Leu Ser Asn Glu Arg Leu Arg Lys Gly Lys Pro Ala Met
530 535 540
Gly Phe Leu Asn Pro Trp Ile Tyr Lys Lys Gly Tyr Lys Gly Phe Thr
545 550 555 560
Asp Ile Val Asp Gly Arg Asn Tyr Gly Cys Gln Gly Phe Ser Leu Gln
565 570 575
Asp Thr Leu Ala Pro Glu Ile Pro Gly Ala Gly Trp Glu Ala Val Lys
580 585 590
Gly Trp Asp Pro Val Ser Gly Phe Gly Thr Pro Leu Tyr Asp Lys Leu
595 600 605
Glu Lys Leu Ala Leu
610
<210> 238
<211> 610
<212> PRT
<213> 太瑞斯梭孢壳霉
<400> 238
Met Leu Gln Gln Leu Phe Val Thr Ala Ala Leu Ala Ala Leu Gly Gln
1 5 10 15
Cys Gly Leu Thr Val Leu Glu Ser Leu Pro Ala Gln Pro Arg Gly Trp
20 25 30
Thr Arg Val Gly Asp Ala Ser Pro Asp His Thr Leu Lys Leu Arg Ile
35 40 45
Ala Leu His Gln Pro Asn Glu Ala Leu Phe Glu Arg Thr Leu Tyr Glu
50 55 60
Ile Ser Asp Pro Phe His Ala Lys Tyr Gly Gln His Leu Ser Arg Asp
65 70 75 80
Glu Leu Ser Ala Leu Met Ala Pro Arg Ala Glu Ser Thr Ser Ala Val
85 90 95
Val Ser Trp Leu Arg Asp Ala Gly Ile Ala Ser Ser Asn Ile Glu Glu
100 105 110
Asp Gly Glu Trp Ile His Val Lys Met Thr Val Arg Glu Ala Ser Ala
115 120 125
Leu Leu Asp Ala Asp Phe Gly Leu Trp Ala His Asp Gly Thr Asn Val
130 135 140
Lys Arg Val Arg Ala Leu Lys Tyr Ser Val Pro Asp Glu Val Val Glu
145 150 155 160
His Val Lys Met Val Ala Pro Ile Val Arg Phe Gly Gln Met Arg Pro
165 170 175
Gln Arg Ser Gln Ile Phe Glu Val Val Asp Ser Lys Thr Pro Glu Pro
180 185 190
Lys Ala Ala Ala Ile Pro Pro Gln Thr Leu Asn Ala Thr Ala Cys Asn
195 200 205
Ala Thr Ile Thr Pro Glu Cys Leu Arg Ala Leu Tyr Asn Val Gly Ser
210 215 220
Tyr Gln Ala Asp Pro Ser Glu His Ser Leu Phe Gly Val Ser Gly Tyr
225 230 235 240
Leu Glu Glu Trp Ala Lys Tyr Asp Gln Leu Glu Leu Phe Thr His Thr
245 250 255
Tyr Ala Pro Tyr Ala Ala Glu Ser Asn Phe Thr Ala Val Leu Val Asn
260 265 270
Gly Gly Val Asn Asn Gln Gly Pro Ser Thr Gln Asp Asp Ile Glu Ala
275 280 285
Asn Leu Asp Ile Gln Tyr Ala Val Ala Met Ser Tyr Lys Thr Pro Ile
290 295 300
Asn Tyr Tyr Ser Thr Gly Gly Arg Gly Pro Val Val Pro Asp Leu Thr
305 310 315 320
Glu Pro Asp Pro Asn Asp Ser Ser Asn Glu Pro Tyr Leu Asp Phe Phe
325 330 335
Gly Tyr Leu Leu Lys Leu Pro Asp Ser Glu Leu Pro Gln Thr Leu Ser
340 345 350
Thr Ser Tyr Gly Glu Asp Glu Gln Ser Val Pro Arg Ala Phe Ala Glu
355 360 365
Lys Val Cys Gln Met Ile Gly Gln Leu Gly Ala Arg Gly Val Ser Val
370 375 380
Ile Phe Ser Ser Gly Asp Ser Gly Val Gly Ser Gly Cys Gln Thr Asn
385 390 395 400
Asp Gly Lys Asn Thr Thr Arg Phe Leu Pro Ile Phe Pro Ala Ala Cys
405 410 415
Pro Tyr Val Thr Ser Val Gly Ala Thr Arg Tyr Val Asn Pro Glu Tyr
420 425 430
Ala Val Ser Phe Ser Ser Gly Gly Phe Ser Asp Ile Phe Pro Arg Pro
435 440 445
Leu Tyr Gln Glu Leu Ala Val Thr Ser Tyr Leu Thr Gln His Leu Gly
450 455 460
Lys Arg Trp Ala Gly Leu Tyr Asn Pro Gly Gly Arg Gly Phe Pro Asp
465 470 475 480
Val Ala Ala Gln Gly Val Arg Tyr His Val Phe Ser Gly Gly Arg Asp
485 490 495
Val Leu Val Ser Gly Thr Ser Ala Ser Ala Pro Leu Phe Ala Ser Leu
500 505 510
Val Ser Leu Leu Asn Asn Ala Arg Leu Ala Arg Gly Leu Pro Pro Leu
515 520 525
Gly Phe Leu Asn Pro Trp Leu Tyr Ala Val Gly Trp Ala Gly Leu Thr
530 535 540
Asp Ile Gln His Gly Gly Ser Thr Gly Cys Thr Ala Asn Asp Ser Ser
545 550 555 560
Ser Gly Val Pro Thr Pro Tyr Val Pro Tyr Ala Ser Trp Asn Ala Thr
565 570 575
Pro Gly Trp Asp Pro Val Thr Gly Leu Gly Thr Pro Asn Leu Glu Lys
580 585 590
Leu Leu Asp Leu Ser Thr Pro Gly Trp Arg Leu Pro Arg Ile Gly Asp
595 600 605
Trp Lys
610
<210> 239
<211> 623
<212> PRT
<213> 稻瘟病菌
<400> 239
Met Glu Pro Thr Glu Pro Tyr Pro Tyr Pro Arg Gly Pro Thr Pro Pro
1 5 10 15
Phe Val Ile Ala Tyr Ala Ala Leu Ala Thr Val Leu Pro Leu Ser Ala
20 25 30
Ser Ala Ala Ser Asn Val Val Val Glu Ser Leu Pro Ala Val Pro Glu
35 40 45
Gly Trp Glu Lys Ile Gln Asp Ala Asp Pro Asp Ser Lys Ile Arg Leu
50 55 60
Lys Val Ala Leu Arg Gln Pro Asn Leu Ala Leu Phe Glu Arg Glu Met
65 70 75 80
Tyr Ala Val Ser Ser Pro Leu Ser Ala Arg Tyr Gly Lys His Leu Lys
85 90 95
Arg Glu Glu Val Arg Asp Leu Met Lys Pro Asp Pro Ala Ser Thr Ser
100 105 110
Thr Val Met Gln Trp Leu Glu Asn Ala Gly Ile Pro Ser Gln Ala Ile
115 120 125
His Glu Asp Ser Asp Trp Ile Asn Ile Val Thr Ser Val Arg Asn Ala
130 135 140
Ser Ala Leu Leu Lys Ala Asp Phe Gly Val Tyr Gly His Ala Asn Ser
145 150 155 160
Asp Leu Lys Lys Ile Arg Ala Leu Glu Tyr Ser Val Pro Asp Asn Val
165 170 175
Arg Asp His Ile Thr Met Val Ala Pro Ile Ile Arg Phe Gly Glu Ile
180 185 190
Lys Pro Lys Arg Ser Ile Val Lys Ser Val Glu Asp Ser Lys Gly Ser
195 200 205
Ile Gln Ala Ala Glu Val Pro Thr Gly Gln Leu Asp Val Ala Ala Cys
210 215 220
Asn Lys Thr Ile Thr Pro Glu Cys Leu Arg Ala Leu Tyr Gln Ile Gly
225 230 235 240
Asp Tyr Lys Ala Thr Gly Ser Pro Lys Ser Arg Phe Gly Ile Ala Gly
245 250 255
Tyr Leu Glu Glu Tyr Ala Arg Tyr Ser Glu Leu Glu Lys Phe Gln Glu
260 265 270
Val Tyr Ala Pro Tyr Thr Leu Gly Ser Asn Phe Ser Val Val Thr Ile
275 280 285
Asn Gly Gly Leu Asn Asp Gln Ser Ser Thr Ala Asp Ser Val Glu Ala
290 295 300
Asn Leu Asp Met Gln Tyr Gly Val Ala Leu Gly Tyr Asn Thr Asn Ile
305 310 315 320
Val Tyr Tyr Ser Thr Gly Gly Arg Gly Pro Leu Val Pro Asp Leu Asp
325 330 335
Gln Pro Asn Gly Asn Asp Ser Ser Asn Glu Pro Tyr Leu Asp Phe Leu
340 345 350
Glu Tyr Met Lys Asn Leu Pro Asp Asp Glu Leu Pro Gln Thr Leu Thr
355 360 365
Thr Ser Tyr Gly Glu Asn Glu Gln Ser Val Pro Arg Glu Phe Ala Val
370 375 380
Lys Val Cys Asn Met Phe Gly Glu Leu Gly Ala Arg Gly Val Ser Ile
385 390 395 400
Phe Phe Ser Ser Gly Asp Glu Gly Pro Gly Ser Ala Cys Gln Ser Asn
405 410 415
Asp Gly Lys Asn Thr Thr Arg Phe Asp Pro Ala Phe Pro Ala Ala Cys
420 425 430
Pro Tyr Val Thr Ala Val Gly Gly Thr Arg Tyr Val Ala Pro Glu Thr
435 440 445
Gly Ala Ala Leu Ala Gly Gly Gly Phe Ser Asp Ile Trp Pro Gln Pro
450 455 460
Glu Trp Gln Lys Thr Ala Val Glu Lys Tyr Leu Ser Ile Leu Gly Asp
465 470 475 480
Lys Trp Lys Gly Leu Tyr Asn Pro Ser Gly Arg Ala Tyr Pro Asp Ile
485 490 495
Ala Gly Gln Ala Tyr Ser Tyr His Val Phe Asp Ser Gly Lys Asp Val
500 505 510
Leu Val Ala Gly Thr Ser Ala Ser Ser Pro Ser Val Ala Ala Ile Phe
515 520 525
Ala Leu Leu Asn Asp Ala Arg Leu Ala Ala Asn Gln Pro Pro Met Gly
530 535 540
Trp Ile Asn Pro Trp Leu Tyr Glu Ile Gly Leu Lys Gly Leu Thr Asp
545 550 555 560
Ile Thr Val Gly Gly Ser Arg Gly Cys Thr Gly Lys Ser Ile Tyr Ser
565 570 575
Gly Leu Pro Ala Pro Lys Val Pro Phe Ala Ser Trp Asn Ala Thr Glu
580 585 590
Gly Trp Asp Pro Val Thr Gly Leu Gly Thr Pro Leu Phe Asn Lys Leu
595 600 605
Leu Glu Leu Ser Thr Pro Gly Ala Gln Ala Gln Lys Ile Ala Asn
610 615 620
<210> 240
<211> 388
<212> PRT
<213> 里氏木霉
<400> 240
Met Ala Trp Leu Lys Lys Leu Ala Leu Val Leu Leu Ala Ile Val Pro
1 5 10 15
Tyr Ala Thr Ala Ser Pro Ala Leu Ser Pro Arg Ser Arg Glu Ile Leu
20 25 30
Ser Leu Glu Asp Leu Glu Ser Glu Asp Lys Tyr Val Ile Gly Leu Lys
35 40 45
Gln Gly Leu Ser Pro Thr Asp Leu Lys Lys His Leu Leu Arg Val Ser
50 55 60
Ala Val Gln Tyr Arg Asn Lys Asn Ser Thr Phe Glu Gly Gly Thr Gly
65 70 75 80
Val Lys Arg Thr Tyr Ala Ile Gly Asp Tyr Arg Ala Tyr Thr Ala Val
85 90 95
Leu Asp Arg Asp Thr Val Arg Glu Ile Trp Asn Asp Thr Leu Glu Lys
100 105 110
Pro Pro Trp Gly Leu Ala Thr Leu Ser Asn Lys Lys Pro His Gly Phe
115 120 125
Leu Tyr Arg Tyr Asp Lys Ser Ala Gly Glu Gly Thr Phe Ala Tyr Val
130 135 140
Leu Asp Thr Gly Ile Asn Ser Lys His Val Asp Phe Glu Gly Arg Ala
145 150 155 160
Tyr Met Gly Phe Ser Pro Pro Lys Thr Glu Pro Thr Asp Ile Asn Gly
165 170 175
His Gly Thr His Val Ala Gly Ile Ile Gly Gly Lys Thr Phe Gly Val
180 185 190
Ala Lys Lys Thr Gln Leu Ile Gly Val Lys Val Phe Leu Asp Asp Glu
195 200 205
Ala Thr Thr Ser Thr Leu Met Glu Gly Leu Glu Trp Ala Val Asn Asp
210 215 220
Ile Thr Thr Lys Gly Arg Gln Gly Arg Ser Val Ile Asn Met Ser Leu
225 230 235 240
Gly Gly Pro Tyr Ser Gln Ala Leu Asn Asp Ala Ile Asp His Ile Ala
245 250 255
Asp Met Gly Ile Leu Pro Val Ala Ala Ala Gly Asn Lys Gly Ile Pro
260 265 270
Ala Thr Phe Ile Ser Pro Ala Ser Ala Asp Lys Ala Met Thr Val Gly
275 280 285
Ala Ile Asn Ser Asp Trp Gln Glu Thr Asn Phe Ser Asn Phe Gly Pro
290 295 300
Gln Val Asn Ile Leu Ala Pro Gly Glu Asp Val Leu Ser Ala Tyr Val
305 310 315 320
Ser Thr Asn Thr Ala Thr Arg Val Leu Ser Gly Thr Ser Met Ala Ala
325 330 335
Pro His Val Ala Gly Leu Ala Leu Tyr Leu Met Ala Leu Glu Glu Phe
340 345 350
Asp Ser Thr Gln Lys Leu Thr Asp Arg Ile Leu Gln Leu Gly Met Lys
355 360 365
Asn Lys Val Val Asn Leu Met Thr Asp Ser Pro Asn Leu Ile Ile His
370 375 380
Asn Asn Val Lys
385
<210> 241
<211> 411
<212> PRT
<213> 深绿木霉
<400> 241
Met Ala Trp Phe Lys His Val Ala Leu Leu Leu Ile Ala Ala Leu Pro
1 5 10 15
Arg Ile Thr Ala Leu Pro Val Asn Asp Ala Asn Asp Ala Asn Asp Ala
20 25 30
Asn Glu Asn Ser Ile Ser Ala Glu Gly Arg Tyr Ile Val Thr Leu Gln
35 40 45
Asn Asn Ile Pro Met Pro Glu Val Arg Asn His Met Ser Arg Val Ala
50 55 60
Ala Ile Gln Tyr Arg Asn Ser Gly Asn His Lys Leu Pro His Thr Gly
65 70 75 80
Ile Glu Lys Ala Tyr Ala Ile Gly Glu Phe Arg Ala Tyr Val Gly Ala
85 90 95
Phe Asp Leu Asp Thr Leu Gln Met Ile Arg Asn Asp Thr Arg Val Ala
100 105 110
Glu Ile Glu Pro Asp Thr Val Val Ser Ile Arg Glu Glu Thr Ser Tyr
115 120 125
Lys Ala Ala Ser Gln Ser Asn Ala Pro Trp Gly Leu Ala Thr Leu Ser
130 135 140
Ser Lys Arg Arg Asn Thr Gly Thr Tyr His Tyr Asp Thr Ser Ala Gly
145 150 155 160
Asn Gly Thr Phe Ala Tyr Val Leu Asp Ser Gly Ile Asn Val Asn His
165 170 175
Ile Glu Phe Glu Gly Arg Ala Ser Asn Gly Tyr Asn Ala Val Asp Gly
180 185 190
Asp Phe Thr Asp Ile Gln Gly His Gly Thr His Cys Ala Gly Ile Ile
195 200 205
Gly Ser Lys Ser Tyr Gly Val Ala Lys Lys Ala Gln Leu Ile Ala Val
210 215 220
Lys Thr Phe Arg Asn Gln Glu Thr Leu Met Ser Thr Val Met Asp Ala
225 230 235 240
Tyr Gln Trp Ser Ala Asn Asp Ile Arg Thr Lys Asn Arg Val Gly Leu
245 250 255
Ala Val Ile Asn Met Ser Leu Gly Gly Ser Tyr Ser Arg Ala Met Asn
260 265 270
His Ala Val Glu Ser Ala Phe Asp Met Gly Ile Leu Thr Ile Val Ala
275 280 285
Ala Gly Asn Glu Gly Arg Pro Ala Arg Leu Val Ser Pro Ala Ser Ala
290 295 300
Ala Asn Ala Phe Thr Val Gly Ala Ile Asp Ala Asp Trp His Gln Trp
305 310 315 320
Glu His Ser Asn Phe Gly Pro Glu Val Asn Ile Phe Ala Pro Gly Val
325 330 335
Asn Ile Glu Ser Thr Tyr Ile Ser Thr Asp Met Ala Thr Arg Lys Leu
340 345 350
Ser Gly Thr Ser Met Ala Ala Pro His Val Ala Gly Leu Ala Leu Tyr
355 360 365
Leu Cys Ala Leu Glu Lys Ile Asp Asn Pro Ile Thr Leu Arg Glu Arg
370 375 380
Ile Leu Asp Leu Gly Thr Arg Gly Arg Ile His Lys Leu Arg Gly Glu
385 390 395 400
Ser Pro Asn Leu Ile Ala His Asn Asn Leu His
405 410
<210> 242
<211> 407
<212> PRT
<213> 绿木霉
<400> 242
Met Ala Trp Phe Lys Lys Leu Ala Leu Leu Leu Ile Thr Val Ser Pro
1 5 10 15
Cys Val Thr Ala Tyr Pro Phe Asn Lys Thr Gly Arg Arg Leu Ala Leu
20 25 30
Asn Leu Asp Ala Leu Glu Pro Glu Ala Lys Tyr Ile Val Thr Leu Gln
35 40 45
Lys Asp Leu Thr Pro Thr Gln Phe Lys Asn His Ile Ala Lys Val Asn
50 55 60
Gly Thr Tyr Tyr Arg Ser Ser Gln Asp Asn Lys Gln Ser Ser Pro Ser
65 70 75 80
Gly Leu Glu Arg Thr Tyr Ala Ile Gly Gly Tyr Arg Ala Tyr Ser Gly
85 90 95
Thr Phe Asp Lys Asp Thr Ile Ala Ser Leu Trp Ser Asp Ala Val Val
100 105 110
Ala Glu Ile Glu Leu Asp Asp Tyr Phe Thr Val Gln Lys Leu Val Thr
115 120 125
Gln Arg His Ala Pro Trp Gly Leu Ala Ala Leu Ser Ser Lys Lys Pro
130 135 140
Gly Ser Glu Ser Tyr Arg Tyr Asp Lys Ser Ala Gly Asn Gly Thr Phe
145 150 155 160
Ala Tyr Val Ile Asp Thr Gly Ile Asn Thr Lys His Val Glu Phe Gly
165 170 175
Gly Arg Ala Ser Val Gly Tyr Ser Val Ile Ala Asp Asp Phe Ser Asp
180 185 190
Asn Ser Gly His Gly Thr His Val Ser Gly Ile Ile Gly Gly Lys Thr
195 200 205
Phe Gly Val Ala Lys Gln Thr Glu Ile Ile Gly Val Lys Val Phe Leu
210 215 220
Asn Asn Gly Gly Glu Asn Ser Asp Val Ile Asp Gly Leu Gln Trp Ala
225 230 235 240
Val Arg Asp Ile Leu Ala Lys Gly Arg Gln Ser Arg Ser Val Ile Asn
245 250 255
Met Ser Leu Gly Gly Pro Tyr Ser Tyr Ala Leu Asn His Ala Val Asp
260 265 270
Ala Ala Phe Glu Met Gly Ile Leu Val Val Val Ala Ala Gly Asn Asp
275 280 285
Ala Glu Pro Ala Arg Tyr Ser Ser Pro Ala Ser Ser Ser Gln Ala Leu
290 295 300
Thr Val Gly Ala Ile Asp Ile Gln Trp Arg Arg Cys Gly Phe Ser Asn
305 310 315 320
Phe Gly Pro Glu Val Asn Ile Glu Ala Pro Gly Glu Asp Ile Glu Ser
325 330 335
Ala Phe Ile Ala Thr Asp Asn Ala Thr Ala Ile Leu Asp Gly Thr Ser
340 345 350
Met Ala Thr Pro His Ile Thr Gly Leu Ala Leu Tyr Leu Ala Ala Leu
355 360 365
Glu Lys Thr Lys Thr Ala Lys Glu Leu Arg Asp Lys Val Leu Glu Val
370 375 380
Ala Ile Lys Asp Lys Ala Lys Asp Leu Thr Leu Glu Thr Pro Asn Leu
385 390 395 400
Ile Ala His Asn Gly Ala Arg
405
<210> 243
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 243
gtaacgccag ggttttccca gtcacgacgg tttaaacgta ttgcgatgag cagcaga 57
<210> 244
<211> 43
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 244
atccacttaa cgttactgaa atctggtctc ctaacccacc aag 43
<210> 245
<211> 43
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 245
ctccttcaat atcatcttct gtctgtgaaa tgaggtccct tcc 43
<210> 246
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 246
gcggataaca atttcacaca ggaaacagcg tttaaaccaa acgcagcaga aaccata 57
<210> 247
<211> 51
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 247
gatttcagta acgttaagtg gatgcggccg cgacagaaga tgatattgaa g 51
<210> 248
<211> 50
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 248
gacagaagat gatattgaag gaggcggccg cttaagtgga tcccggtgac 50
<210> 249
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 249
ggtgggttag gagaccagat ttcagtaacg ttaagtggat gcggccgcct agcatcgact 60
actgctgc 68
<210> 250
<211> 46
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 250
gcagcagtag tcgatgctag gcgcgccatg caaagataca catcaa 46
<210> 251
<211> 46
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 251
ttgatgtgta tctttgcatg gcgcgcctag catcgactac tgctgc 46
<210> 252
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 252
agggacctca tttcacagac agaagatgat attgaaggag gcggccgcgg ctgatgaggc 60
tgagagag 68
<210> 253
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 253
tcgctgtaac gaacttctgt 20
<210> 254
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 254
cattgttgac ctccactagc 20
<210> 255
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 255
gctgctgatc ggacattttt 20
<210> 256
<211> 18
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 256
gtttctggca gctggact 18
<210> 257
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 257
tcgctgtaac gaacttctgt 20
<210> 258
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 258
tgcgtcgccg tctcgctcct 20
<210> 259
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 259
cgacgatcta cagccatctg 20
<210> 260
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 260
acccaaagcg tccttcatta 20
<210> 261
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 261
gtaacgccag ggttttccca gtcacgacgg tttaaacttc agttgtggca tctcagc 57
<210> 262
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 262
gccaagccca aaaagtgctc cttcaatatc atcttctgtc gcggccgcgg gaagcaagtt 60
tcgaagtg 68
<210> 263
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 263
cccgtcaccg agatctgatc cgtcaccggg atccacttaa gcggccgcaa tggatgaggt 60
gtggcttc 68
<210> 264
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 264
gcggataaca atttcacaca ggaaacagcg tttaaactcc tcaccgaaga gcaagtc 57
<210> 265
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 265
gtaacgccag ggttttccca gtcacgacgg tttaaacttc agttgtggca tctcagc 57
<210> 266
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 266
cattgttgac ctccactagc 20
<210> 267
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 267
cgtcaccgag atctgatcc 19
<210> 268
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 268
gcggataaca atttcacaca ggaaacagcg tttaaactcc tcaccgaaga gcaagtc 57
<210> 269
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 269
gacgtggtac gacaacatcg 20
<210> 270
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 270
tatcaaggta ccggggacag 20
<210> 271
<211> 67
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 271
gattaagttg ggtaacgcca gggttttccc agtcacgacg gtttaaacac ctcatgaggg 60
actatgg 67
<210> 272
<211> 69
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 272
gcgctggcaa cgagagcaga gcagcagtag tcgatgctag gcggccgcca agaagaggca 60
gagggtaat 69
<210> 273
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 273
caaccagccg cagcctcagc ctctctcagc ctcatcagcc gcggccgcct atacatactg 60
atgataca 68
<210> 274
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 274
tggaattgtg agcggataac aatttcacac aggaaacagc gtttaaacgc cccatgtatg 60
gactctac 68
<210> 275
<211> 22
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 275
ctcagaaagg ttgtagttgt ga 22
<210> 276
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 276
ccatgagctt gaacaggtaa 20
<210> 277
<211> 22
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 277
gatgttgtgt tttcagtctg ca 22
<210> 278
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 278
catcctcaag gcctcagac 19
<210> 279
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 279
atgttcatcg ctggcgtcg 19
<210> 280
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 280
ctaaacgtaa gagcaggtca a 21
<210> 281
<211> 67
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 281
gattaagttg ggtaacgcca gggttttccc agtcacgacg gtttaaacgc tactacgcga 60
gcaagtg 67
<210> 282
<211> 66
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 282
ggaactgtcg gcgattggga gaatttcgtg cgatcgcggc ggccgccgga tgaagatgtg 60
cagttg 66
<210> 283
<211> 69
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 283
agggaacata tcaccctcgg gcatttttca tttggtaggc ggccgctaag atatcttcaa 60
gcttatgcg 69
<210> 284
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 284
cggatgaaga tgtgcagttg 20
<210> 285
<211> 61
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 285
tgtctcactt ccacccatct caactgcaca tcttcatccg agcaacaaca tgaggttcga 60
a 61
<210> 286
<211> 69
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 286
cctatgttgt gtggaattgt gagcggataa caatttcaca gtttaaacac aacgcatgtc 60
cagcttttg 69
<210> 287
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 287
gcttggcatc acggaagct 19
<210> 288
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 288
ccatgagctt gaacaggtaa 20
<210> 289
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 289
ttgacaagaa aggtccggtt g 21
<210> 290
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 290
catcctcaag gcctcagac 19
<210> 291
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 291
atggatgcta tccgagccag 20
<210> 292
<211> 22
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 292
ctattcatac tcaacagtca ca 22
<210> 293
<211> 58
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 293
gtaacgccag ggttttccca gtcacgacgg tttaaactgc tgttgctgtt tgttgatg 58
<210> 294
<211> 69
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 294
cccgtcaccg agatctgatc cgtcaccggg atccacttaa gcggccgcct gtggtgagat 60
ctccagacg 69
<210> 295
<211> 69
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 295
gccaagccca aaaagtgctc cttcaatatc atcttctgtc gcggccgcac tgtgcccaac 60
aataagcag 69
<210> 296
<211> 55
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 296
gcggataaca atttcacaca ggaaacagcg tttaaaccca aggcgctggc tgtta 55
<210> 297
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 297
gatcgacaaa ggttccagcg 20
<210> 298
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 298
aattgtatca ttccgaggct 20
<210> 299
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 299
ctgtttggcc ctcgaaact 19
<210> 300
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 300
cattgttgac ctccactagc 20
<210> 301
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 301
ttcgccatcc aaatttcttc 20
<210> 302
<211> 18
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 302
gtttctggca gctggact 18
<210> 303
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 303
ctgtttggcc ctcgaaact 19
<210> 304
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 304
tgcgtcgccg tctcgctcct 20
<210> 305
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 305
ttcgccatcc aaatttcttc 20
<210> 306
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 306
catcctcaag gcctcagac 19
<210> 307
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 307
cccaagtcgt ctcagctctc 20
<210> 308
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 308
tcgaaggctt cagtgaggta a 21
<210> 309
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 309
gtaacgccag ggttttccca gtcacgacgg tttaaacatc tcggagtgat gcttcct 57
<210> 310
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 310
gcgctggcaa cgagagcaga gcagcagtag tcgatgctag gcggccgcat cagacgaaac 60
cagacgag 68
<210> 311
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 311
caaccagccg cagcctcagc ctctctcagc ctcatcagcc gcggccgcgc gaatcgagtt 60
gatgattc 68
<210> 312
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 312
gcggataaca atttcacaca ggaaacagcg tttaaacctg gttgggatct gaccact 57
<210> 313
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 313
gcagacaaac agagcaacga 20
<210> 314
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 314
ccatgagctt gaacaggtaa 20
<210> 315
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 315
tagagggtgt cgatggaagc 20
<210> 316
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 316
catcctcaag gcctcagac 19
<210> 317
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 317
ggtctcttct ttgccagcac 20
<210> 318
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 318
tgtcgctgaa ctgaatttgc 20
<210> 319
<211> 67
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 319
gattaagttg ggtaacgcca gggttttccc agtcacgacg gtttaaacgc agtctatccc 60
atccctg 67
<210> 320
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 320
gcgctggcaa cgagagcaga gcagcagtag tcgatgctag gcggccgcgc ggatgatgaa 60
ggaagaag 68
<210> 321
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 321
caaccagccg cagcctcagc ctctctcagc ctcatcagcc gcggccgcaa cagctgttcg 60
cacgcgtg 68
<210> 322
<211> 66
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 322
tggaattgtg agcggataac aatttcacac aggaaacagc gtttaaacgg ctgggcattg 60
gggccg 66
<210> 323
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 323
gattaagttg ggtaacgcca gggttttccc agtcacgacg gtttaaacaa acaaggcaca 60
aaggcctg 68
<210> 324
<211> 67
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 324
gcgctggcaa cgagagcaga gcagcagtag tcgatgctag gcggccgcat ccaaggatga 60
ggagaac 67
<210> 325
<211> 71
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 325
caaccagccg cagcctcagc ctctctcagc ctcatcagcc gcggccgcac ctaatggttt 60
cttcgttttt c 71
<210> 326
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 326
tggaattgtg agcggataac aatttcacac aggaaacagc gtttaaaccg gtccgaaggg 60
tgttttgg 68
<210> 327
<211> 17
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 327
gatgcaccgc tgcggcc 17
<210> 328
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 328
ccatgagctt gaacaggtaa 20
<210> 329
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 329
catcctcaag gcctcagac 19
<210> 330
<211> 18
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 330
ggcgttgctc cccatgcg 18
<210> 331
<211> 18
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 331
atgcggtccg ttgtcgcc 18
<210> 332
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 332
ttactcggag agctcagaga 20
<210> 333
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 333
gtgaatgggt ggcaacatga 20
<210> 334
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 334
ccatgagctt gaacaggtaa 20
<210> 335
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 335
catcctcaag gcctcagac 19
<210> 336
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 336
catcaagttg accaccattg t 21
<210> 337
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 337
atgcggttgt ccgtcctcc 19
<210> 338
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 338
ttaaccggaa gggttgccgt 20
<210> 339
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 339
ctgtttggcc ctcgaaact 19
<210> 340
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 340
ccatgagctt gaacaggtaa 20
<210> 341
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 341
ttcgccatcc aaatttcttc 20
<210> 342
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 342
catcctcaag gcctcagac 19
<210> 343
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 343
gcagacaaac agagcaacga 20
<210> 344
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 344
ccatgagctt gaacaggtaa 20
<210> 345
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 345
tagagggtgt cgatggaagc 20
<210> 346
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 346
catcctcaag gcctcagac 19
<210> 347
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 347
ggtctcttct ttgccagcac 20
<210> 348
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 348
tgtcgctgaa ctgaatttgc 20
<210> 349
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 349
gcagacaaac agagcaacga 20
<210> 350
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 350
ccatgagctt gaacaggtaa 20
<210> 351
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 351
tagagggtgt cgatggaagc 20
<210> 352
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 352
catcctcaag gcctcagac 19
<210> 353
<211> 22
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 353
ctcagaaagg ttgtagttgt ga 22
<210> 354
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 354
ccatgagctt gaacaggtaa 20
<210> 355
<211> 22
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 355
gatgttgtgt tttcagtctg ca 22
<210> 356
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 356
catcctcaag gcctcagac 19
<210> 357
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 357
atgttcatcg ctggcgtcg 19
<210> 358
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 358
ctaaacgtaa gagcaggtca a 21
<210> 359
<211> 22
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 359
ctcagaaagg ttgtagttgt ga 22
<210> 360
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 360
ccatgagctt gaacaggtaa 20
<210> 361
<211> 22
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 361
gatgttgtgt tttcagtctg ca 22
<210> 362
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 362
catcctcaag gcctcagac 19
<210> 363
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 363
gcttggcatc acggaagct 19
<210> 364
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 364
ccatgagctt gaacaggtaa 20
<210> 365
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 365
ttgacaagaa aggtccggtt g 21
<210> 366
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 366
catcctcaag gcctcagac 19
<210> 367
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 367
atggatgcta tccgagccag 20
<210> 368
<211> 22
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 368
ctattcatac tcaacagtca ca 22
<210> 369
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 369
gtaacgccag ggttttccca gtcacgacgg tttaaactca ggtcaaccac cgaggac 57
<210> 370
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 370
cccgtcaccg agatctgatc cgtcaccggg atccacttaa gcggccgctg aatgggatgg 60
ttcgattg 68
<210> 371
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 371
gccaagccca aaaagtgctc cttcaatatc atcttctgtc gcggccgcag gtagacgctt 60
tgcgagtg 68
<210> 372
<211> 55
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 372
gcggataaca atttcacaca ggaaacagcg tttaaactga actgacgcgg actga 55
<210> 373
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 373
aagttccctt cctctggcag caatcgaacc atcccattca gcggccgcct agcatcgact 60
actgctgc 68
<210> 374
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 374
catgcaaaga tacacatcaa 20
<210> 375
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 375
tgattgtacc ccagctgcga ttgatgtgta tctttgcatg gcggccgctc aatgttgact 60
gccccagg 68
<210> 376
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 376
gcacttctta gatacacaca cactcgcaaa gcgtctacct ggcgcgcctg aatgggatgg 60
ttcgattg 68
<210> 377
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 377
ggcaggtcgc agagcaagac a 21
<210> 378
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 378
ttgacaagaa aggtccggtt g 21
<210> 379
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 379
gattcatcac aggggcagtc 20
<210> 380
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 380
tgcgtcgccg tctcgctcct 20
<210> 381
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 381
acgccgttgc tgagccttg 19
<210> 382
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 382
ttaggcgacc tctttttcca 20
<210> 383
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 383
gagcccatca tcaacacctc 20
<210> 384
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 384
tgccaaggtc gtagacgga 19
<210> 385
<211> 58
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 385
ggtaacgcca gggttttccc agtcacgacg gtttaaacgt cgagccccct ggacacct 58
<210> 386
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 386
gcgctggcaa cgagagcaga gcagcagtag tcgatgctag gcggccgcca tcgccgtcgc 60
ggacatga 68
<210> 387
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 387
tgattgtacc ccagctgcga ttgatgtgta tctttgcatg gcggccgctc gacgttgtat 60
ctgcactc 68
<210> 388
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 388
gtacgttctg attgccaact acggaccaga ccagggctcc ggcgcgccca tcgccgtcgc 60
ggacatga 68
<210> 389
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 389
ggagccctgg tctggtccgt 20
<210> 390
<211> 58
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 390
agcggataac aatttcacac aggaaacagc gtttaaacac gcgcttcaac atgcccca 58
<210> 391
<211> 60
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 391
gctggccgct gggaatagcg tcatgtccgc gacggcgatg gaattcggtc tgaaggacgt 60
<210> 392
<211> 22
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 392
gttgagagaa gttgttggat tg 22
<210> 393
<211> 62
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 393
aaccaaagac tttttgatca atccaacaac ttctctcaac atgaagattt cctcgatcct 60
tg 62
<210> 394
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 394
tcagcgccgt aacctctgc 19
<210> 395
<211> 61
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 395
tgatggtgat gaggcggaaa agcagaggtt acggcgctga ggatccactt aacgttactg 60
a 61
<210> 396
<211> 71
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 396
tctctcaaag gaagaatccc ttcagggttg cgtttccagt gcggccgctc tccttctaga 60
aagaaggatt a 71
<210> 397
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 397
actggaaacg caaccctgaa 20
<210> 398
<211> 58
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 398
tctgattgcc aactacggac cagaccaggg ctccggcgcg gcggccgcta gatctacg 58
<210> 399
<211> 66
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 399
gtacacttgt ttagaggtaa tccttctttc tagaaggaga gcggccgcgg agccctggtc 60
tggtcc 66
<210> 400
<211> 59
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 400
gcgctggcaa cgagagcaga gcagcagtag tcgatgctag aagctgacgg gcgtcaacg 59
<210> 401
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 401
tagcatcgac tactgctgc 19
<210> 402
<211> 70
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 402
gtacgttctg attgccaact acggaccaga ccagggctcc gcggccgcca tgcaaagata 60
cacatcaatc 70
<210> 403
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 403
gattcatcac aggggcagtc 20
<210> 404
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 404
ccgctctcaa actgcccaaa 20
<210> 405
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 405
acgtgaagtt gcccatcaa 19
<210> 406
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 406
ccatgagctt gaacaggtaa 20
<210> 407
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 407
gaccaatggc ttcacgaagt 20
<210> 408
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 408
ttaggcgacc tctttttcca 20
<210> 409
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 409
acgtgaagtt gcccatcaa 19
<210> 410
<211> 24
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 410
taacttgtac gctctcagtt cgag 24
<210> 411
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 411
gaccaatggc ttcacgaagt 20
<210> 412
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 412
ttaggcgacc tctttttcca 20
<210> 413
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 413
cagcagcacc gcatccacca 20
<210> 414
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 414
gccgaatcgc tggttgccct 20
<210> 415
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 415
atggaagcca tcctccagg 19
<210> 416
<211> 18
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 416
tggatcatgt tggcgacg 18
<210> 417
<211> 17
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 417
gtaagacgcc ccgtctc 17
<210> 418
<211> 58
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 418
ggtaacgcca gggttttccc agtcacgacg gtttaaacgg aggctgcgac accgtctg 58
<210> 419
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 419
gcgctggcaa cgagagcaga gcagcagtag tcgatgctag gcggccgccc ggcctgaaac 60
gacctccc 68
<210> 420
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 420
cccgtcaccg agatctgatc cgtcaccggg atccacttaa gcggccgcga gagagaaaca 60
aaacagtg 68
<210> 421
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 421
acattccgac cgtttactga tccaagccgt gcaaccgact ggcgcgcccc ggcctgaaac 60
gacctccc 68
<210> 422
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 422
agtcggttgc acggcttgga 20
<210> 423
<211> 58
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 423
agcggataac aatttcacac aggaaacagc gtttaaacga gacggacgcc tgcaccac 58
<210> 424
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 424
tgattgtacc ccagctgcga ttgatgtgta tctttgcatg gcgatcgcga cagaagatga 60
tattgaag 68
<210> 425
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 425
ttaagtggat cccggtgacg 20
<210> 426
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 426
cctatgagtc gtttacccag a 21
<210> 427
<211> 18
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 427
tggccgagtc tatgcgta 18
<210> 428
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 428
ggagttgctt taatgtcggg 20
<210> 429
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 429
ttaggcgacc tctttttcca 20
<210> 430
<211> 17
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 430
gtaagacgcc ccgtctc 17
<210> 431
<211> 18
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 431
tggatcatgt tggcgacg 18
<210> 432
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 432
gtcgaagatg tcctcgagat 20
<210> 433
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 433
ggagttgctt taatgtcggg 20
<210> 434
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 434
ttaggcgacc tctttttcca 20
<210> 435
<211> 18
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 435
tagtccatgc cgaactgc 18
<210> 436
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 436
ccggacctgc accgcaagtt 20
<210> 437
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 437
agggcaatgt cgcccagcac 20
<210> 438
<211> 17
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 438
gacctgcacc gcaagtt 17
<210> 439
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 439
gtcgagcgtc tgatattcac 20
<210> 440
<211> 17
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 440
gacggagacc tcccaca 17
<210> 441
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 441
gtaacgccag ggttttccca gtcacgacgg tttaaaccat aaacttgcgc agtcgaa 57
<210> 442
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 442
gcgctggcaa cgagagcaga gcagcagtag tcgatgctag gcggccgcct tctaggatgg 60
agcgcttg 68
<210> 443
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 443
caaccagccg cagcctcagc ctctctcagc ctcatcagcc gcggccgcag acggcttctt 60
ccaaaaca 68
<210> 444
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 444
gcggataaca atttcacaca ggaaacagcg tttaaacccc cagggaggct attctac 57
<210> 445
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 445
ctttccaagc gtttgagtcc 20
<210> 446
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 446
ccatgagctt gaacaggtaa 20
<210> 447
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 447
gcgtgtttta tcctggtgct 20
<210> 448
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 448
catcctcaag gcctcagac 19
<210> 449
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 449
cacctccgtc gatgagtttt 20
<210> 450
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 450
agaagaaggt ggtggtggtg 20
<210> 451
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 451
gtaacgccag ggttttccca gtcacgacgg tttaaacgtt tgagcattct cccaagc 57
<210> 452
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 452
gcgctggcaa cgagagcaga gcagcagtag tcgatgctag gcggccgccg ccattttgaa 60
gaagatgc 68
<210> 453
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 453
caaccagccg cagcctcagc ctctctcagc ctcatcagcc gcggccgcat gctccctcgt 60
cattaagc 68
<210> 454
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 454
gcggataaca atttcacaca ggaaacagcg tttaaacaca acaccttctc cgacacc 57
<210> 455
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 455
atgcccaagt ttcgtacctg 20
<210> 456
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 456
ccatgagctt gaacaggtaa 20
<210> 457
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 457
ggcgcattca gaagaagaac 20
<210> 458
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 458
catcctcaag gcctcagac 19
<210> 459
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 459
cacttgatga acgctggcta 20
<210> 460
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 460
cgtaatggcg ttgttgacag 20
<210> 461
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 461
gtaacgccag ggttttccca gtcacgacgg tttaaacgag gcagccaaaa agtgaag 57
<210> 462
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 462
gcgctggcaa cgagagcaga gcagcagtag tcgatgctag gcggccgctg aaagaaggca 60
ggaccagt 68
<210> 463
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 463
caaccagccg cagcctcagc ctctctcagc ctcatcagcc gcggccgcaa gaggctcgga 60
caaagaca 68
<210> 464
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 464
gcggataaca atttcacaca ggaaacagcg tttaaacgat cgtggtgcac gagacta 57
<210> 465
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 465
gcactgcgtt gcctttctat 20
<210> 466
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 466
ccatgagctt gaacaggtaa 20
<210> 467
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 467
gaaagcatgg ctcgtttctc 20
<210> 468
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 468
catcctcaag gcctcagac 19
<210> 469
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 469
acccggctca actagctaca 20
<210> 470
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 470
agctggcctt tcgttacaga 20
<210> 471
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 471
gtaacgccag ggttttccca gtcacgacgg tttaaacttg gtttgaacag ctgcaag 57
<210> 472
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 472
gcgctggcaa cgagagcaga gcagcagtag tcgatgctag gcggccgctt tgcagcaaga 60
tgtcgttc 68
<210> 473
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 473
caaccagccg cagcctcagc ctctctcagc ctcatcagcc gcggccgcgc tgtgaagacg 60
ggcttatc 68
<210> 474
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 474
gcggataaca atttcacaca ggaaacagcg tttaaaccaa gaacagcatc gaggaca 57
<210> 475
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 475
gggcgacgac gagttttat 19
<210> 476
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 476
ccatgagctt gaacaggtaa 20
<210> 477
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 477
gaatggatca agtcgctgct 20
<210> 478
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 478
catcctcaag gcctcagac 19
<210> 479
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 479
ctcaggctct gcttggattc 20
<210> 480
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 480
atgccaaaaa gactgctgct 20
<210> 481
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 481
gtaacgccag ggttttccca gtcacgacgg tttaaacgcc tccctggtat tcagaca 57
<210> 482
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 482
gcgctggcaa cgagagcaga gcagcagtag tcgatgctag gcggccgcga cgccagaaag 60
aaatgctc 68
<210> 483
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 483
caaccagccg cagcctcagc ctctctcagc ctcatcagcc gcggccgcga cctggtcagc 60
tgctcttt 68
<210> 484
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 484
gcggataaca atttcacaca ggaaacagcg tttaaactgg aaccacatcg acttcac 57
<210> 485
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 485
aaccaccttg tcaccgtctc 20
<210> 486
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 486
ccatgagctt gaacaggtaa 20
<210> 487
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 487
gtcgtcgagg ctgctttatc 20
<210> 488
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 488
catcctcaag gcctcagac 19
<210> 489
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 489
gatctcgaat ccgaggacaa 20
<210> 490
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 合成构建体
<400> 490
ccggtagcgt tagagagacg 20
<210> 491
<211> 417
<212> PRT
<213> 嗜热毁丝霉
<400> 491
Met His Leu Thr Pro Ala Leu Val Ala Ala Thr Cys Ala Val Glu Val
1 5 10 15
Cys Ala Gly Val Leu Pro Arg Ser Ser Ser Thr Pro Thr Thr Phe Gly
20 25 30
Ser Gly Thr Leu Ser Leu Lys Gln Val Arg Asn Pro Asn Phe Val Arg
35 40 45
Asn Gly Pro Val Gln Leu Ala Arg Ile Tyr His Lys Tyr Gly Val Pro
50 55 60
Leu Pro His Asp Leu Arg Glu Ala Val Ala Arg Phe Arg Ala Glu Ile
65 70 75 80
Arg Lys Arg Ser Asn Gly Ser Thr Glu Thr Asn Pro Glu Thr Asn Asp
85 90 95
Val Glu Tyr Leu Thr Pro Val Ser Ile Gly Thr Pro Pro Gln Val Leu
100 105 110
Asn Leu Asp Phe Asp Thr Gly Ser Ser Asp Leu Trp Val Phe Ser Ser
115 120 125
Glu Thr Arg Ser Ser Asp Val Gln Gly Gln Thr Ile Tyr Asp Pro Asn
130 135 140
Glu Ser Ser Thr Ala Gln Lys Leu Gln Gly Tyr Ser Trp Gln Ile Ser
145 150 155 160
Tyr Gly Asp Gly Ser Ser Ser Ser Gly Asp Val Tyr Thr Asp Ala Val
165 170 175
Thr Val Gly Gly Leu Thr Val Pro Ser Gln Ala Val Glu Val Ala Arg
180 185 190
Arg Val Ser Asp Glu Phe Thr Ser Asp Pro Asn Asn Asp Gly Leu Leu
195 200 205
Gly Leu Gly Phe Ser Ser Ile Asn Thr Val Gln Pro Val Pro Gln Lys
210 215 220
Thr Phe Phe Asp Asn Ala Lys Ala Asp Leu Asp Ala Pro Ile Phe Thr
225 230 235 240
Ala Asp Leu Lys Ala Ser Ala Pro Gly Phe Phe Asn Phe Gly Tyr Ile
245 250 255
Asp His Gly Ala Tyr Thr Gly Glu Ile Thr Tyr Met Pro Val Asp Ser
260 265 270
Ser Asp Gly Phe Trp Ala Trp Thr Ser Pro Gly Tyr Ala Val Gly Ser
275 280 285
Gly Ser Phe Lys Arg Thr Thr Ile Gln Gly Ile Ala Asp Thr Gly Thr
290 295 300
Ser Leu Phe Leu Leu Pro Ser Ser Val Val Ser Ala Tyr Tyr Gly Gln
305 310 315 320
Ile Ser Gly Ala Lys Tyr Asp Ser Ile Gln Gly Gly Tyr Thr Leu Pro
325 330 335
Cys Ser Gly Ser Val Pro Asp Phe Ala Phe Gly Ile Gly Asp Ser Asn
340 345 350
Thr Thr Ile Ser Val Pro Gly Asp Tyr Val Arg Tyr Ala Ala Thr Asp
355 360 365
Ser Ser Gly Ile Ile Cys Phe Gly Gly Ile Gln Ala Asn Thr Gly Ile
370 375 380
Gly Phe Ser Ile Phe Gly Asp Val Ala Leu Lys Ala Ala Phe Val Val
385 390 395 400
Phe Asp Gly Ala Lys Gln Gln Leu Gly Trp Ala Ser Lys Pro Leu Pro
405 410 415
Ser
<210> 492
<211> 396
<212> PRT
<213> 产黄青霉
<400> 492
Met Val Val Phe Ser Lys Val Thr Ala Ser Leu Ala Cys Phe Ser Ala
1 5 10 15
Val Val Ser Ala Ala Ala Val Pro Val Lys Ser Pro Arg Gln Gly Phe
20 25 30
Ser Val Asn Gln Val Gln Lys Thr Val Thr Gly Thr Arg Thr Val Asn
35 40 45
Leu Pro Gly Val Tyr Ala Asn Ala Leu Ala Lys Tyr Gly Ala Thr Val
50 55 60
Pro Ala Asn Val His Ala Ala Ala Val Ser Gly Ser Ala Ile Thr Thr
65 70 75 80
Pro Glu Glu Asn Asp Val Glu Tyr Leu Thr Pro Val Lys Ile Gly Glu
85 90 95
Ser Thr Leu Asn Leu Asp Phe Asp Thr Gly Ser Ala Asp Leu Trp Val
100 105 110
Phe Ser Thr Glu Leu Ser Ser Ala Glu Gln Ser Gly His Asp Val Tyr
115 120 125
Asp Val Ser Ser Ser Gly Lys Lys Leu Thr Gly Ala Ser Trp Ser Ile
130 135 140
Ser Tyr Gly Asp Gly Ser Gly Ala Ser Gly Asp Val Tyr Lys Asp Thr
145 150 155 160
Val Thr Val Gly Gly Val Lys Ala Thr Gly Gln Ala Val Glu Ala Ala
165 170 175
Lys Lys Ile Ser Gln Gln Phe Val Gln Asp Lys Ser Asn Asp Gly Leu
180 185 190
Leu Gly Leu Ala Phe Ser Ser Ile Asn Thr Val Ser Pro Lys Pro Gln
195 200 205
Thr Thr Phe Phe Asp Thr Val Lys Ser Asp Leu Asp Lys Pro Leu Phe
210 215 220
Ala Val Thr Leu Lys His Gly Ala Pro Gly Thr Tyr Asp Phe Gly Tyr
225 230 235 240
Ile Asp Lys Lys Lys Phe Thr Gly Ser Leu Thr Tyr Thr Asp Val Asp
245 250 255
Asn Ser Gln Gly Phe Trp Ser Phe Thr Ala Asp Ser Tyr Lys Val Gly
260 265 270
Thr Gly Ser Ala Gly Pro Ser Ile Glu Gly Ile Ala Asp Thr Gly Thr
275 280 285
Thr Leu Leu Leu Leu Asp Asp Gly Val Val Ser Asp Tyr Tyr Lys Lys
290 295 300
Val Asp Gly Ala Lys Asn Asn Tyr Ser Ala Gly Gly Tyr Val Phe Pro
305 310 315 320
Cys Asp Ala Asp Leu Pro Asp Phe Thr Val Thr Ile Gly Ser Tyr Asp
325 330 335
Ala Val Val Pro Gly Lys His Ile Lys Tyr Ala Pro Val Thr Thr Gly
340 345 350
Ser Ser Ser Cys Phe Gly Gly Ile Gln Ser Asn Ser Gly Ile Gly Phe
355 360 365
Ser Ile Phe Gly Asp Ile Phe Leu Lys Ser Gln Tyr Val Val Phe Asp
370 375 380
Ala Glu Gly Pro Arg Leu Gly Phe Ala Ala Gln Ala
385 390 395
<210> 493
<211> 394
<212> PRT
<213> 黑曲霉
<400> 493
Met Val Val Phe Ser Lys Thr Ala Ala Leu Val Leu Gly Leu Ser Ser
1 5 10 15
Ala Val Ser Ala Ala Pro Ala Pro Thr Arg Lys Gly Phe Thr Ile Asn
20 25 30
Gln Ile Ala Arg Pro Ala Asn Lys Thr Arg Thr Ile Asn Leu Pro Gly
35 40 45
Met Tyr Ala Arg Ser Leu Ala Lys Phe Gly Gly Thr Val Pro Gln Ser
50 55 60
Val Lys Glu Ala Ala Ser Lys Gly Ser Ala Val Thr Thr Pro Gln Asn
65 70 75 80
Asn Asp Glu Glu Tyr Leu Thr Pro Val Thr Val Gly Lys Ser Thr Leu
85 90 95
His Leu Asp Phe Asp Thr Gly Ser Ala Asp Leu Trp Val Phe Ser Asp
100 105 110
Glu Leu Pro Ser Ser Glu Gln Thr Gly His Asp Leu Tyr Thr Pro Ser
115 120 125
Ser Ser Ala Thr Lys Leu Ser Gly Tyr Thr Trp Asp Ile Ser Tyr Gly
130 135 140
Asp Gly Ser Ser Ala Ser Gly Asp Val Tyr Arg Asp Thr Val Thr Val
145 150 155 160
Gly Gly Val Thr Thr Asn Lys Gln Ala Val Glu Ala Ala Ser Lys Ile
165 170 175
Ser Ser Glu Phe Val Gln Asp Thr Ala Asn Asp Gly Leu Leu Gly Leu
180 185 190
Ala Phe Ser Ser Ile Asn Thr Val Gln Pro Lys Ala Gln Thr Thr Phe
195 200 205
Phe Asp Thr Val Lys Ser Gln Leu Asp Ser Pro Leu Phe Ala Val Gln
210 215 220
Leu Lys His Asp Ala Pro Gly Val Tyr Asp Phe Gly Tyr Ile Asp Asp
225 230 235 240
Ser Lys Tyr Thr Gly Ser Ile Thr Tyr Thr Asp Ala Asp Ser Ser Gln
245 250 255
Gly Tyr Trp Gly Phe Ser Thr Asp Gly Tyr Ser Ile Gly Asp Gly Ser
260 265 270
Ser Ser Ser Ser Gly Phe Ser Ala Ile Ala Asp Thr Gly Thr Thr Leu
275 280 285
Ile Leu Leu Asp Asp Glu Ile Val Ser Ala Tyr Tyr Glu Gln Val Ser
290 295 300
Gly Ala Gln Glu Ser Glu Glu Ala Gly Gly Tyr Val Phe Ser Cys Ser
305 310 315 320
Thr Asn Pro Pro Asp Phe Thr Val Val Ile Gly Asp Tyr Lys Ala Val
325 330 335
Val Pro Gly Lys Tyr Ile Asn Tyr Ala Pro Ile Ser Thr Gly Ser Ser
340 345 350
Thr Cys Phe Gly Gly Ile Gln Ser Asn Ser Gly Leu Gly Leu Ser Ile
355 360 365
Leu Gly Asp Val Phe Leu Lys Ser Gln Tyr Val Val Phe Asn Ser Glu
370 375 380
Gly Pro Lys Leu Gly Phe Ala Ala Gln Ala
385 390
<210> 494
<211> 386
<212> PRT
<213> 构巢曲霉
<400> 494
Met Val Val Phe Ser Lys Val Ala Ala Ala Ala Phe Gly Leu Ser Ala
1 5 10 15
Val Ala Ser Ala Met Pro Ala Ala Pro Pro Arg Gln Gly Phe Thr Ile
20 25 30
Asn Gln Leu Thr Arg Ala Ile Pro Lys Arg Thr Ile Asn Leu Pro Ala
35 40 45
Ile Tyr Ala Asn Ala Leu Ser Lys Tyr Gly Gly Asn Val Pro Pro His
50 55 60
Ile Gln Asp Ala Met Ala His Gly Ser Ala Val Thr Thr Pro Glu Gln
65 70 75 80
Tyr Asp Val Glu Tyr Leu Thr Pro Val Ala Val Gly Gly Thr Thr Met
85 90 95
Asn Leu Asp Phe Asp Thr Gly Ser Ala Asp Leu Trp Val Phe Ser Asn
100 105 110
Glu Leu Pro Ser Ser Gln Thr Thr Gly His Ser Val Tyr Lys Pro Ser
115 120 125
Asp Asn Gly Thr Arg Met Ser Gly Tyr Ser Trp Glu Ile Ser Tyr Gly
130 135 140
Asp Gly Ser Ser Ala Gly Gly Asp Val Tyr Arg Asp Thr Val Thr Val
145 150 155 160
Gly Gly Val Thr Ala Pro Gly Gln Ala Val Glu Ala Ala Ser His Ile
165 170 175
Ser Glu Gln Phe Thr Arg Asp Gln Asn Asn Asp Gly Leu Leu Gly Leu
180 185 190
Ala Phe Ser Ser Ile Asn Thr Val Gln Pro Lys Ser Gln Thr Thr Phe
195 200 205
Phe Asp Ser Val Lys Ser Gln Leu Glu Ser Pro Leu Phe Ala Val Thr
210 215 220
Leu Lys His Gln Ala Pro Gly Ser Tyr Asp Phe Gly Tyr Ile Asp Gln
225 230 235 240
Ser Lys Tyr Thr Gly Glu Leu Thr Tyr Thr Asp Val Asp Asn Ser Gln
245 250 255
Gly Phe Trp Met Phe Ser Ala Thr Ala Gly Glu Thr Asp Phe Asp Ala
260 265 270
Ile Ala Asp Thr Gly Thr Thr Leu Ile Met Ile Asp Gln Ser Ile Ala
275 280 285
Glu Asp Tyr Tyr Ser Gln Val Pro Leu Ala Phe Asn Asn Phe Phe Tyr
290 295 300
Gly Gly Trp Thr Phe Pro Cys Ser Ala Glu Leu Pro Ser Phe Thr Val
305 310 315 320
Thr Ile Asn Gly Tyr Asp Ala Val Val Pro Gly Glu His Ile Lys Tyr
325 330 335
Ala Pro Val Thr Asp Gly Ser Ser Thr Cys Phe Gly Gly Ile Gln Asp
340 345 350
Asn Gln Gly Leu Pro Phe Ser Ile Leu Gly Asp Val Phe Leu Lys Ser
355 360 365
Gln Tyr Val Val Phe Asp Ser Glu Gly Pro Gln Leu Gly Phe Ala Pro
370 375 380
Gln Ala
385
<210> 495
<211> 434
<212> PRT
<213> 粗糙链孢霉
<400> 495
Met Leu Leu Phe Pro Thr Ile Leu Thr Ala Ala Leu Ala Ala Thr Gly
1 5 10 15
Met Ala Ala Ala Ile Pro Ser Arg Asp Asp Thr Thr Ala Asn Lys Gly
20 25 30
Thr Ala Ser Leu Leu Gln Val Arg Asn Pro Ser Phe Glu Phe Arg His
35 40 45
Gly Pro Leu Ala Leu Ala Lys Ala Tyr Gln Lys Phe Gly Ala Pro Met
50 55 60
Pro Glu Asp Leu Arg Ala Ala Ile Ala Arg Phe Arg Gln Asn Gln Lys
65 70 75 80
Arg Thr Thr Gly Thr Ile Ala Thr Asp Pro Glu Lys His Asp Val Glu
85 90 95
Tyr Leu Thr Pro Ile Ser Val Gly Thr Pro Ser Gln Asp Leu Val Val
100 105 110
Asp Phe Asp Thr Gly Ser Ser Asp Leu Trp Val Phe Ser Thr Glu Met
115 120 125
Ser Thr Ser Asp Ile Lys Gly Gln Thr Val Tyr Asp Pro Asn Asn Ser
130 135 140
Ser Thr Ser Glu Lys Val Gln Gly Ser Thr Trp Lys Ile Thr Tyr Gly
145 150 155 160
Asp Gly Ser Ser Ser Ser Gly Asp Val Tyr Leu Asp Thr Val Thr Ile
165 170 175
Gly Asn Leu Thr Val Pro Ser Gln Ala Val Glu Ala Ala Lys Lys Val
180 185 190
Ser Ser Glu Phe Thr Asp Asp Ser His Asn Asp Gly Leu Leu Gly Leu
195 200 205
Gly Phe Ser Ala Ile Asn Ala Val Glu Pro Thr Pro Gln Asn Thr Phe
210 215 220
Phe Asp Asn Ile Lys Gly Ser Leu Asp Ala Pro Leu Phe Thr Val Asp
225 230 235 240
Leu Lys His Gly Thr Pro Gly Ser Phe Asn Phe Gly Tyr Ile Asp Pro
245 250 255
Ala Ala Tyr Ile Gly Asn Ile Ser Trp Thr Pro Val Asp Ser Ser Gln
260 265 270
Gly Tyr Trp Gly Phe Thr Ser Pro Gly Tyr Ala Val Gly Thr Gly Ala
275 280 285
Phe Arg Asn His Ser Ile Ser Gly Ile Ala Asp Thr Gly Thr Thr Leu
290 295 300
Leu Leu Leu Pro Lys Ser Val Val Ser Ala Tyr Tyr Lys Glu Ile Gln
305 310 315 320
Gly Ala Gln Tyr Asp Ser Asp Gln Gly Gly Tyr Ile Phe Pro Cys Ser
325 330 335
Pro Thr Pro Pro Asp Phe Val Phe Gly Val Asn Lys Gly Ile Val Thr
340 345 350
Val Pro Gly Asp Met Val Ser Tyr Ala Pro Ala Asp Ser Ala Asn Gln
355 360 365
Asn Cys Phe Gly Gly Ile Gln Thr Asp Thr Gly Ile Gly Phe Ser Ile
370 375 380
Phe Gly Asp Val Ala Leu Lys Thr Ser Phe Val His Leu His Gly Ser
385 390 395 400
Ile Val Pro Gly Tyr Tyr Ala Asp Cys Ala Met Arg Phe Asn Arg Met
405 410 415
Leu Arg Ser Tyr Ser Asn Asp Gln Leu Val Asp Phe Ser Ser Ser Gly
420 425 430
Pro Leu
<210> 496
<211> 466
<212> PRT
<213> 嗜热毁丝霉
<400> 496
Met Asp Ala Leu Phe Glu Thr His Ala Lys Leu Arg Lys Arg Met Ala
1 5 10 15
Leu Tyr Arg Val Arg Ala Val Pro Asn Gln Asn Tyr Gln Arg Asp Gly
20 25 30
Thr Lys Ser Tyr Val Ser Val Leu Asn Arg Phe Gly Phe Gln Pro Thr
35 40 45
Lys Pro Gly Pro Tyr Phe Gln Ile Phe Glu Glu Ser Glu Glu Ala Pro
50 55 60
Ser Met Ser Ala Ala Pro Gly Val Lys Pro Gly His Val Trp Gln Gly
65 70 75 80
Leu Phe Lys Lys Leu Lys Asp Gln Glu Glu Pro Gly Glu Val Thr Ala
85 90 95
Glu Asp Gln Gln Asn Asp Ser Glu Tyr Leu Cys Glu Val Met Ile Gly
100 105 110
Thr Ala Trp Thr Ala Glu Arg Gln Ile Val Lys Met Asp Phe Asp Thr
115 120 125
Gly Leu Ala Asp Phe Trp Val Ser Gln Lys Ser Phe Asp Pro Lys Lys
130 135 140
Ser Val Thr Trp Gln Leu Ala Lys Asp Lys Ser Trp Lys Val Gln Tyr
145 150 155 160
Gly Asp Gly Ser Ser Ala Ser Gly Ile Val Gly His Asp Ile Leu Ile
165 170 175
Ile Gly Gly Ile Gln Ile Lys Arg Gln Ala Ile Glu Ile Ala Thr Glu
180 185 190
Met Ser Ala Gln Phe Ser Glu Gly Thr Met Asp Gly Ile Leu Gly Leu
195 200 205
Ala Phe Ser Lys Leu Asn Thr Val Gln Thr Asp Gly Lys Pro Asp Pro
210 215 220
Gln Arg Thr Val Val Asp Asn Met Met Ala Gln Asp Asp Ile Pro Pro
225 230 235 240
Glu Ala Glu Leu Phe Ser Thr Ala Leu Tyr Ser Asn Arg Glu Asp Asp
245 250 255
Gln Arg Ser Phe Tyr Thr Phe Gly Trp Ile Asp Glu Asp Leu Val Lys
260 265 270
Ala Ser Gly Glu Glu Ile Val Trp Thr Asp Val Asp Asn Ser Glu Gly
275 280 285
Phe Trp Met Phe Ser Ser Glu His Val Thr Ile Asp Gly Gln Gln Val
290 295 300
Arg Ile Glu Gly Asn Lys Ala Ile Ala Asp Thr Gly Thr Ser Leu Val
305 310 315 320
Leu Val Ser Asp Gln Val Cys Asp Ala Leu Tyr Ala His Ile Pro Ser
325 330 335
Ala Glu Tyr Ser Glu Glu Tyr Gln Gly Trp Thr Phe Pro Gln Glu Thr
340 345 350
Glu Val Asp Lys Leu Pro Glu Phe Ser Ile Ala Ile Gly Asp Lys Glu
355 360 365
Phe Val Leu Gln Lys Glu Asp Leu Ile Phe Ala Pro Ala Asp Glu Arg
370 375 380
Val Phe Tyr Gly Ser Val Gln Ser Arg Gly Glu Asn Pro Phe Asp Ile
385 390 395 400
Leu Gly Ile Ala Phe Leu Lys Ser Ile Tyr Ala Ile Trp Asp Gln Gly
405 410 415
His Lys Arg Phe Gly Ala Val Pro Lys Met Glu Ala Phe Val Pro Pro
420 425 430
Thr Lys Tyr Asp Arg Pro Arg Leu Thr Asp Gln Asp Arg Lys Asp Leu
435 440 445
Gly Val Thr Ile Gly Tyr Gly Asp Ile Ser Ser Thr Phe Phe Glu Lys
450 455 460
Arg Ala
465
<210> 497
<211> 419
<212> PRT
<213> 产黄青霉
<400> 497
Met Thr Arg Ile Lys Leu Ile Ala Asn Pro His Tyr Gln Lys Ser Gly
1 5 10 15
Thr Lys Ser Tyr Leu Tyr Ala Met Arg Lys Tyr Arg Phe Thr Pro Thr
20 25 30
Lys Asp Gly Pro Tyr Phe Leu Gly Thr Thr Met Val Gln Thr Gly Arg
35 40 45
Gln Phe Thr Asn Lys Pro Val Gly Gly Arg Ala Arg Leu Gln Gln Val
50 55 60
Leu Gln Lys Lys Asp Ala Val Ser Asp Glu Val Gly Gln Val Gly Val
65 70 75 80
His Asp Val Gln Asn Asp Ser Met Tyr Leu Ala Glu Ile Gly Ile Gly
85 90 95
Thr Pro Ala Gln Thr Leu Asn Leu Asp Phe Asp Thr Gly Ser Ala Asp
100 105 110
Leu Trp Val Trp Ser Thr Glu Leu Ser Ser Lys Ile Leu Ser Ala Asn
115 120 125
Lys Asn His Arg Val Phe Asp Pro Thr Lys Ser Ser Thr Phe Glu Glu
130 135 140
Lys Asp Gly Ser Thr Trp Gln Ile Lys Tyr Gly Asp Gly Ser Phe Ala
145 150 155 160
Ser Gly Thr Val Gly Asn Asp Asn Val Thr Ile Gly Gly Leu Val Val
165 170 175
Lys Gly Gln Ala Val Gly Ile Ala Asp Ile Leu Ser Glu Gln Phe Ala
180 185 190
Gln Gly Ala Gly Asp Gly Leu Leu Gly Leu Ala Phe Ser Asn Ile Asn
195 200 205
Thr Val Lys Pro Glu Ala Val Ala Met Pro Val Glu Asn Met Ile Ser
210 215 220
Gln Ala Asp Ile Pro Lys Ser Ala Glu Leu Phe Thr Ala Lys Leu Gly
225 230 235 240
Ser Trp Arg Asp Thr Asp Glu Pro Asp Lys Gly Glu Ser Phe Tyr Thr
245 250 255
Phe Gly Tyr Val Asp Gln Asp Thr Val Lys Ala Ala Glu Ser Glu Ile
260 265 270
Tyr Tyr Thr Pro Val Asp Asn Ser Gln Gly Phe Trp Met Phe Asp Ser
275 280 285
Thr Ser Ala Thr Val Asn Gly Lys Ser Ile Ala Arg Ala Gly Asn Lys
290 295 300
Ala Ile Ala Asp Thr Gly Thr Thr Leu Ala Leu Val Asp Asp Glu Thr
305 310 315 320
Cys Gln Ala Val Tyr Asp Ala Ile Pro Gly Ala Lys Tyr Asp Asn Ala
325 330 335
Ser Gln Gly Trp Ile Phe Pro Ser Asn Thr Pro Ala Asp Lys Leu Pro
340 345 350
Val Val Ser Phe Ala Val Gly Glu Lys Gln Phe Val Val Gln Lys Glu
355 360 365
Asp Leu Gly Phe Ala Glu Ala Lys Ser Gly Tyr Val Tyr Gly Gly Ile
370 375 380
Gln Ser Arg Gly Thr Met Thr Met Asp Ile Leu Gly Asp Thr Phe Leu
385 390 395 400
Lys Ala Ile Tyr Ala Val Ser Asp Met Leu Phe Phe Gly Val His Ser
405 410 415
Cys Thr Asn
<210> 498
<211> 420
<212> PRT
<213> 米曲霉
<400> 498
Met Ala Ser Lys Ile Phe Leu Ile Glu Asn Pro Asn Tyr Cys Lys Ser
1 5 10 15
Gly Ile Lys Ser Tyr Val His Leu Ile Arg Lys Tyr Arg Leu His Pro
20 25 30
Thr Lys Asn Gly Pro Tyr Ser Ile Gly Arg Ala Ile His Gln Thr Gly
35 40 45
Arg Pro Phe Thr Asp Lys Pro Ile Gly Gly Arg Val Arg Phe His Asp
50 55 60
Val Met Arg Lys Gln Thr Ser Asp Asp Gln Leu Gln Gln Val Glu Lys
65 70 75 80
Asp Asp Ile Gln Asn Asp Ala Ala Phe Phe Met Pro Val Ser Ile Gly
85 90 95
Thr Pro Ala Gln Thr Phe Asn Leu Val Leu Asp Thr Ala Ser Val Asp
100 105 110
Phe Trp Val Arg Ser Ser Glu Leu Pro Ala Asp Thr Leu Pro Lys Gly
115 120 125
Glu Glu Glu Ser His Thr Phe Asp Pro Glu Lys Ser Ser Thr Phe Lys
130 135 140
Asn Ile Glu Asp Ser Pro Trp Lys Val Thr Tyr Val Asp Gly Ser Ser
145 150 155 160
Val Ser Gly Ser Ile Gly Thr Glu Asp Ile Thr Ile Gly Gly Val Ala
165 170 175
Phe Lys Thr Gln Pro Val Gln Leu Ala Lys Thr Met Ser Pro Met Phe
180 185 190
Thr Gln Phe Ser Ala Asp Gly Val Leu Gly Leu Ala Phe Gly His Ile
195 200 205
Asn His Pro Gln Gln Asp Ile Lys Ala Leu Ala Glu Arg Leu Asn Thr
210 215 220
Arg Asp Asp Arg Glu Gln Ser Ala Lys Leu Phe Thr Ala Lys Phe Gly
225 230 235 240
Ser Trp Gly Asp Ser Asp Leu Lys Lys Ser Phe Cys Thr Phe Gly Ser
245 250 255
Ile Asp Glu Asp Ala Val Arg Tyr Cys Gly His Asp Val His His Thr
260 265 270
Pro Ile Asp Asn Ser Arg Gly Tyr Trp Met Phe Glu Ser Pro Ser Ala
275 280 285
Thr Val Gly Gly Lys Pro Met Ser Arg Ser Glu Asn Lys Ala Val Val
290 295 300
Asp Thr Asp Ala Ala Leu Thr Leu Leu Asp Asp Gln Thr Cys Gln Ala
305 310 315 320
Ile Tyr Asp Ser Ile Pro Gly Ala Phe Tyr Asp Ser Glu Thr Gln Gly
325 330 335
Phe Leu Ile Pro Ser Asp Ile Asn Val Asp Gln Leu Pro Val Val Gln
340 345 350
Leu Ala Val Gly Glu Lys Ser Phe Ala Met Ser Lys Glu Ser Leu Met
355 360 365
Phe Ala Glu Ala Lys Pro Gly Tyr Ile Tyr Gly Gly Val Gln Ser Arg
370 375 380
Gly Ser Leu Glu Phe Asp Val Leu Gly Gly Thr Phe Leu Asp Gly Leu
385 390 395 400
Tyr Ala Ile Phe Asp Phe Gly Ser Leu Arg Phe Gly Ala Val Gln Ala
405 410 415
Lys Thr Lys Val
420
<210> 499
<211> 397
<212> PRT
<213> 嗜热毁丝霉
<400> 499
Met Lys Asp Ala Phe Leu Leu Thr Ala Ala Val Leu Leu Gly Ser Ala
1 5 10 15
Gln Gly Ala Val His Lys Met Lys Leu Gln Lys Ile Pro Leu Ser Glu
20 25 30
Gln Leu Glu Ala Val Pro Ile Asn Thr Gln Leu Glu His Leu Gly Gln
35 40 45
Lys Tyr Met Gly Leu Arg Pro Arg Glu Ser Gln Ala Asp Ala Ile Phe
50 55 60
Lys Gly Met Val Ala Asp Val Lys Gly Asn His Pro Ile Pro Ile Ser
65 70 75 80
Asn Phe Met Asn Ala Gln Tyr Phe Ser Glu Ile Thr Ile Gly Thr Pro
85 90 95
Pro Gln Ser Phe Lys Val Val Leu Asp Thr Gly Ser Ser Asn Leu Trp
100 105 110
Val Pro Ser Val Glu Cys Gly Ser Ile Ala Cys Tyr Leu His Ser Lys
115 120 125
Tyr Asp Ser Ser Ala Ser Ser Thr Tyr Lys Lys Asn Gly Thr Ser Phe
130 135 140
Glu Ile Arg Tyr Gly Ser Gly Ser Leu Ser Gly Phe Val Ser Gln Asp
145 150 155 160
Thr Val Ser Ile Gly Asp Ile Thr Ile Gln Gly Gln Asp Phe Ala Glu
165 170 175
Ala Thr Ser Glu Pro Gly Leu Ala Phe Ala Phe Gly Arg Phe Asp Gly
180 185 190
Ile Leu Gly Leu Gly Tyr Asp Arg Ile Ser Val Asn Gly Ile Val Pro
195 200 205
Pro Phe Tyr Lys Met Val Glu Gln Lys Leu Ile Asp Glu Pro Val Phe
210 215 220
Ala Phe Tyr Leu Ala Asp Thr Asn Gly Gln Ser Glu Val Val Phe Gly
225 230 235 240
Gly Val Asp His Asp Lys Tyr Lys Gly Lys Ile Thr Thr Ile Pro Leu
245 250 255
Arg Arg Lys Ala Tyr Trp Glu Val Asp Phe Asp Ala Ile Ser Tyr Gly
260 265 270
Asp Asp Thr Ala Glu Leu Glu Asn Thr Gly Ile Ile Leu Asp Thr Gly
275 280 285
Thr Ser Leu Ile Ala Leu Pro Ser Gln Leu Ala Glu Met Leu Asn Ala
290 295 300
Gln Ile Gly Ala Lys Lys Ser Tyr Thr Gly Gln Tyr Thr Ile Asp Cys
305 310 315 320
Asn Lys Arg Asp Ser Leu Lys Asp Val Thr Phe Asn Leu Ala Gly Tyr
325 330 335
Asn Phe Thr Leu Gly Pro Tyr Asp Tyr Val Leu Glu Val Gln Gly Ser
340 345 350
Cys Ile Ser Thr Phe Met Gly Met Asp Phe Pro Ala Pro Thr Gly Pro
355 360 365
Leu Ala Ile Leu Gly Asp Ala Phe Leu Arg Arg Tyr Tyr Ser Ile Tyr
370 375 380
Asp Leu Gly Ala Asp Thr Val Gly Leu Ala Glu Ala Lys
385 390 395
<210> 500
<211> 398
<212> PRT
<213> 产黄青霉
<400> 500
Met Lys Ser Phe Thr Leu Leu Thr Ala Ser Ala Leu Leu Gly Ser Ala
1 5 10 15
Ala Ala Glu Val His Arg Leu Lys Leu Asn Lys Val Pro Leu Ala Glu
20 25 30
Gln Leu Asn Thr His Asn Ile Asp Ala His Val His Asn Leu Gly Gln
35 40 45
Lys Tyr Met Gly Ile Arg Pro Glu Lys His Gln Asp Leu Phe His Asp
50 55 60
Thr Ser Phe Asn Pro Ala Ala Gly His Asp Val Leu Val Asp Asn Phe
65 70 75 80
Leu Asn Ala Gln Tyr Phe Ser Glu Ile Ser Ile Gly Thr Pro Pro Gln
85 90 95
Thr Phe Lys Val Val Leu Asp Thr Gly Ser Ser Asn Leu Trp Val Pro
100 105 110
Ser Ser Gln Cys Ser Ser Ile Ala Cys Phe Leu His Ser Lys Tyr Asp
115 120 125
Ser Ser Ser Ser Ser Thr Tyr Glu Lys Asn Gly Thr Glu Phe Glu Ile
130 135 140
Arg Tyr Gly Ser Gly Ser Leu Ser Gly Phe Val Ser Arg Asp Thr Leu
145 150 155 160
Gln Ile Gly Asp Leu Lys Val Lys Gly Gln Asp Phe Ala Glu Ala Thr
165 170 175
Asn Glu Pro Gly Leu Ala Phe Ala Phe Gly Arg Phe Asp Gly Ile Leu
180 185 190
Gly Leu Gly Tyr Asp Thr Ile Ser Val Asn Lys Met Val Pro Pro Phe
195 200 205
Tyr His Met Ile Asn Gln Lys Leu Val Asp Glu Pro Val Phe Ala Phe
210 215 220
Tyr Leu Gly Asp Ala Asn Lys Asp Gly Asp Asn Ser Val Ala Thr Phe
225 230 235 240
Gly Gly Ile Asp Glu Ser His Tyr Thr Gly Glu Leu Ile Lys Ile Pro
245 250 255
Leu Arg Arg Lys Ala Tyr Trp Glu Val Glu Leu Asn Ser Ile Ala Leu
260 265 270
Gly Asp Asn Val Ala Glu Leu Glu Asn Thr Gly Val Ile Leu Asp Thr
275 280 285
Gly Thr Ser Leu Ile Ala Leu Pro Ser Thr Met Ala Glu Leu Leu Asn
290 295 300
Lys Glu Ile Gly Ala Thr Lys Gly Phe Thr Gly Gln Tyr Ser Val Glu
305 310 315 320
Cys Asp Lys Arg Asp Ser Leu Pro Asp Leu Thr Phe Thr Leu Gly Gly
325 330 335
His Lys Phe Thr Ile Gly Pro Tyr Asp Tyr Val Leu Glu Val Gln Gly
340 345 350
Ser Cys Ile Ser Ser Phe Met Gly Met Asp Phe Pro Glu Pro Val Gly
355 360 365
Pro Leu Ala Ile Leu Gly Asp Ala Phe Leu Arg Arg Trp Tyr Ser Val
370 375 380
Tyr Asp Val Gly Asn Asn Ala Val Gly Leu Ala Lys Ala Lys
385 390 395
<210> 501
<211> 397
<212> PRT
<213> 米曲霉
<400> 501
Met Lys Ser Thr Leu Val Thr Ala Ser Val Leu Leu Gly Cys Ala Ser
1 5 10 15
Ala Glu Val His Lys Leu Lys Leu Asn Lys Val Pro Val Ser Glu Gln
20 25 30
Phe Asn Leu His Asn Ile Asp Thr His Val Gln Ala Leu Gly Gln Lys
35 40 45
Tyr Met Gly Ile Arg Pro Asn Ile Lys Gln Asp Leu Leu Asn Glu Asn
50 55 60
Pro Ile Asn Asp Met Gly Arg His Asp Val Leu Val Asp Asn Phe Leu
65 70 75 80
Asn Ala Gln Tyr Phe Ser Glu Ile Glu Ile Gly Thr Pro Pro Gln Lys
85 90 95
Phe Lys Val Val Leu Asp Thr Gly Ser Ser Asn Leu Trp Val Pro Ser
100 105 110
Ser Glu Cys Gly Ser Ile Ala Cys Tyr Leu His Asn Lys Tyr Asp Ser
115 120 125
Ser Ser Ser Ser Thr Tyr Gln Lys Asn Gly Ser Glu Phe Ala Ile Lys
130 135 140
Tyr Gly Ser Gly Ser Leu Ser Gly Phe Val Ser Gln Asp Thr Leu Lys
145 150 155 160
Ile Gly Asp Leu Lys Val Lys Asp Gln Leu Phe Ala Glu Ala Thr Ser
165 170 175
Glu Pro Gly Leu Ala Phe Ala Phe Gly Arg Phe Asp Gly Ile Leu Gly
180 185 190
Leu Gly Phe Asp Thr Ile Ser Val Asn Lys Ile Pro Pro Pro Phe Tyr
195 200 205
Ser Met Leu Asp Gln Gly Leu Leu Asp Glu Pro Val Phe Ala Phe Tyr
210 215 220
Leu Gly Asp Thr Asn Lys Glu Gly Asp Asp Ser Val Ala Thr Phe Gly
225 230 235 240
Gly Val Asp Lys Asp His Tyr Thr Gly Glu Leu Val Lys Ile Pro Leu
245 250 255
Arg Arg Lys Ala Tyr Trp Glu Val Asp Leu Asp Ala Ile Ala Leu Gly
260 265 270
Asp Ser Val Ala Glu Leu Asp Asn Thr Gly Val Ile Leu Asp Thr Gly
275 280 285
Thr Ser Leu Ile Ala Leu Pro Thr Thr Leu Ala Glu Leu Ile Asn Lys
290 295 300
Glu Ile Gly Ala Lys Lys Gly Phe Thr Gly Gln Tyr Ser Val Asp Cys
305 310 315 320
Asp Lys Arg Asp Ser Leu Pro Asp Leu Thr Phe Thr Leu Ser Gly Tyr
325 330 335
Asn Phe Thr Ile Gly Pro Tyr Asp Tyr Thr Leu Glu Val Gln Gly Ser
340 345 350
Cys Ile Ser Ala Phe Met Gly Met Asp Phe Pro Glu Pro Val Gly Pro
355 360 365
Leu Ala Ile Leu Gly Asp Ala Phe Leu Arg Lys Trp Tyr Ser Val Tyr
370 375 380
Asp Leu Gly Asn Gly Ala Val Gly Leu Ala Lys Ala Lys
385 390 395
<210> 502
<211> 394
<212> PRT
<213> 构巢曲霉
<400> 502
Met Lys Ala Ser Leu Leu Thr Ala Ser Val Leu Leu Gly Tyr Ala Ser
1 5 10 15
Ala Glu Val His Lys Leu Lys Leu Asn Lys Val Pro Leu Thr Glu Gln
20 25 30
Phe Ile Thr Arg Asn Ile Ala Asp His Ala Asn Ala Leu Gly Gln Lys
35 40 45
Tyr Met Gly Gln Phe Gln Gln His Val Leu Glu Asp Glu Pro Val Asn
50 55 60
Ala Met Arg Gly His Asp Val Leu Val Asp Asn Phe Met Asn Ala Gln
65 70 75 80
Tyr Phe Ser Glu Ile Gln Leu Gly Thr Pro Pro Gln Thr Phe Lys Val
85 90 95
Val Leu Asp Thr Gly Ser Ser Asn Leu Trp Val Pro Ser Ser Glu Cys
100 105 110
Gly Ser Ile Ala Cys Tyr Leu His Gln Lys Phe Asp Ser Ser Ala Ser
115 120 125
Ser Thr Tyr Lys Lys Asn Gly Ser Glu Phe Ala Ile Lys Tyr Gly Ser
130 135 140
Gly Ser Leu Ser Gly Phe Val Ser Arg Asp Asn Leu Gln Ile Gly Asp
145 150 155 160
Leu Lys Val Lys Gly Gln Asp Phe Ala Glu Ala Thr Ser Glu Pro Gly
165 170 175
Leu Ala Phe Ala Phe Gly Arg Phe Asp Gly Ile Leu Gly Leu Gly Phe
180 185 190
Asp Thr Ile Ser Val Asn Arg Ile Val Pro Pro Phe Tyr Asn Met Ile
195 200 205
His Gln Gly Leu Leu Asp Glu Pro Val Phe Ala Phe Tyr Leu Gly Asp
210 215 220
Ala Asn Lys Asp Gly Asp Ser Ser Val Ala Thr Phe Gly Gly Ile Asp
225 230 235 240
Lys Asp His Tyr Glu Gly Glu Leu Ile Lys Ile Pro Leu Arg Arg Lys
245 250 255
Ala Tyr Trp Glu Val Asp Leu Asp Ala Ile Ala Leu Gly Asp Glu Val
260 265 270
Ala Glu Leu Glu Asn Thr Gly Val Ile Leu Asp Thr Gly Thr Ser Leu
275 280 285
Ile Ala Leu Pro Ser Asn Leu Ala Glu Met Ile Asn Thr Glu Ile Gly
290 295 300
Ala Thr Lys Gly Phe Thr Gly Gln Tyr Thr Ile Asp Cys Ala Lys Arg
305 310 315 320
Asp Ser Leu Pro Asp Leu Thr Phe Thr Leu Thr Gly His Asn Phe Thr
325 330 335
Ile Gly Pro Tyr Asp Tyr Thr Leu Glu Val Gln Gly Ser Cys Ile Ser
340 345 350
Ala Phe Met Gly Met Asp Phe Pro Glu Pro Val Gly Pro Leu Ala Ile
355 360 365
Leu Gly Asp Ala Phe Leu Arg Lys Trp Tyr Ser Val Tyr Asp Leu Gly
370 375 380
Asn Gly Ala Val Gly Leu Ala Lys Ala Lys
385 390
<210> 503
<211> 454
<212> PRT
<213> 嗜热毁丝霉
<400> 503
Met Lys Phe Ala Ala Leu Ala Leu Ala Ala Ser Leu Val Ala Ala Ala
1 5 10 15
Pro Arg Val Val Lys Val Asp Pro Ser Asp Ile Lys Pro Arg Arg Leu
20 25 30
Gly Gly Thr Lys Phe Lys Leu Gly Gln Ile His Asn Asp Leu Phe Arg
35 40 45
Gln His Gly Arg Gly Pro Arg Ala Leu Ala Lys Ala Tyr Glu Lys Tyr
50 55 60
Asn Ile Glu Leu Pro Pro Asn Leu Leu Glu Val Val Gln Arg Ile Leu
65 70 75 80
Lys Asp Leu Gly Ile Glu Pro His Ser Lys Lys Ile Pro Gly Ser Lys
85 90 95
Ser Ser Tyr Gly Asn Gly Ala Pro Tyr Thr Asn Glu Thr Asp Asp Ser
100 105 110
Gly Glu Val Ser Ala Ile Pro Gln Leu Phe Asp Val Glu Tyr Leu Ala
115 120 125
Pro Val Gln Ile Gly Thr Pro Pro Gln Thr Leu Met Leu Asn Phe Asp
130 135 140
Thr Gly Ser Ser Asp Leu Trp Val Phe Ser Ser Glu Thr Pro Ser Arg
145 150 155 160
Gln Gln Asn Gly Gln Lys Ile Tyr Lys Ile Glu Glu Ser Ser Thr Ala
165 170 175
Arg Arg Leu Ser Asn His Thr Trp Ser Ile Gln Tyr Gly Asp Gly Ser
180 185 190
Arg Ser Ala Gly Asn Val Tyr Leu Asp Thr Val Ser Val Gly Gly Val
195 200 205
Asn Val Phe Asn Gln Ala Val Glu Ser Ala Thr Phe Val Ser Ser Ser
210 215 220
Phe Val Thr Asp Ala Ala Ser Ser Gly Leu Leu Gly Leu Gly Phe Asp
225 230 235 240
Ser Ile Asn Thr Val Lys Pro Thr Lys Gln Lys Thr Phe Ile Ser Asn
245 250 255
Ala Leu Glu Ser Leu Glu Met Gly Leu Phe Thr Ala Asn Leu Lys Lys
260 265 270
Ala Glu Pro Gly Asn Tyr Asn Phe Gly Phe Ile Asp Glu Thr Glu Phe
275 280 285
Val Gly Pro Leu Ser Phe Ile Asp Val Asp Ser Thr Asp Gly Phe Trp
290 295 300
Gln Phe Asp Ala Thr Gly Tyr Ser Ile Gln Leu Pro Glu Pro Ser Gly
305 310 315 320
Asn Ile Thr Gly Thr Pro Phe Arg Ala Val Ala His Thr Ala Ile Ala
325 330 335
Asp Thr Gly Thr Thr Leu Leu Leu Leu Pro Pro Gly Ile Ala Gln Ala
340 345 350
Tyr Tyr Trp Gln Val Gln Gly Ala Arg Gln Ala Pro Glu Val Gly Gly
355 360 365
Trp Val Met Pro Cys Asn Ala Ser Met Pro Asp Leu Thr Leu His Ile
370 375 380
Gly Thr Tyr Lys Ala Val Ile Pro Gly Glu Leu Ile Pro Tyr Ala Pro
385 390 395 400
Val Asp Thr Asp Asp Met Asp Thr Ala Thr Val Cys Tyr Gly Gly Ile
405 410 415
Gln Ser Ala Ser Gly Met Pro Phe Ala Ile Tyr Gly Asp Ile Phe Phe
420 425 430
Lys Ala Gln Phe Thr Val Phe Asp Val Glu Asn Leu Lys Leu Gly Phe
435 440 445
Ala Pro Lys Pro Glu Leu
450
<210> 504
<211> 545
<212> PRT
<213> 构巢曲霉
<400> 504
Met Ser Val Leu Leu Val Phe Ser Leu Val Thr Phe Ala Leu Gln Gly
1 5 10 15
Leu Cys Ser Leu Ser Leu His Glu Ser Asp Ser Pro His Val Leu Gln
20 25 30
Leu Gly Leu Arg Arg Asn Gln His Asn Asp Pro Val Gly Arg Asp Arg
35 40 45
Arg Arg Phe Lys Arg Gln Thr Gly Thr Val Ala Val Asp Leu His Gly
50 55 60
Asp Ser Met Gly Gly Asp Ile Tyr Ser Thr Asn Leu Thr Ile Gly Asp
65 70 75 80
Pro Pro Gln Ala Val Glu Val Ser Val Asp Thr Gly Ser Ala Asp Leu
85 90 95
Trp Val Val Tyr Ser Glu Asn Pro Val Cys Asn Val Arg Gly Ala Arg
100 105 110
Cys Asp Asp Ser Gly Thr Tyr Asp Pro Thr Ala Ser Thr Ser Phe Asp
115 120 125
Gly Leu Ser Asp Glu Phe Ala Ile Glu Tyr Gly Asp Ser Ser Trp Ala
130 135 140
Glu Gly Tyr Tyr Gly Ile Asp Thr Leu Thr Val Ala Asp Ala Glu Val
145 150 155 160
Ser Glu Val Gln Phe Ala Val Ala Val Glu Ser Ser Ile Asp Lys Gly
165 170 175
Ile Leu Gly Ile Gly Tyr Ser Thr Asn Val Val Ser Thr Tyr Arg Tyr
180 185 190
Pro Asn Leu Pro Glu Arg Leu Val Ala Ser Asn Ile Thr Ser Ser Asn
195 200 205
Ala Tyr Ser Leu Trp Leu Asn Arg Leu Gly Ser Asp Glu Gly Thr Ile
210 215 220
Leu Phe Gly Gly Val Asn Thr Ala His Tyr Thr Gly Pro Leu Arg Thr
225 230 235 240
Leu Pro Val Val Arg Tyr Asn Gly His Tyr Ile His Leu Trp Leu Thr
245 250 255
Leu Thr Gly Met Gly Val Glu Ser Ala Ser Asp Asp Ile Thr Lys Ser
260 265 270
Tyr Ser Glu Thr Arg Ser Thr Thr Gly Glu Gln Glu Phe Pro Phe Val
275 280 285
Ala Leu Leu Asp Ser Gly Ala Thr Leu Thr Tyr Leu Pro Ser Asn Ile
290 295 300
Val Ala Gln Ile Phe Ser Asp Leu Asp Val His Leu Tyr Glu Pro Glu
305 310 315 320
Gln Phe Gly Tyr Val Pro Cys Asp Thr Tyr Leu Val Gly Arg Glu Asp
325 330 335
Tyr Asn Leu Thr Phe Thr Phe Ser Gly Val Thr Ile Arg Val Pro Leu
340 345 350
Arg Glu Leu Val Leu Arg Asp Ala Ile Ser Gly Pro Gly Arg Asp Ala
355 360 365
Leu Gln Leu Pro Asn Asn Ala Asp Glu Glu Ser Cys Leu Phe Gly Ile
370 375 380
Leu Pro Asn Thr Asp Leu Phe Pro Ile Leu Gly Asp Thr Phe Leu Arg
385 390 395 400
Ser Ala Tyr Val Val Phe Asp Leu Asp Asn Asn Glu Ile Ser Leu Ala
405 410 415
Gln Ala Asn Thr Ala Pro Gly Asp Asp Arg Ile Leu Glu Ile Gly Ser
420 425 430
Gly Asp Asp Ala Val Pro Glu Ala Glu Asp Val Asp Asp Pro Val Thr
435 440 445
Thr Ala Thr Val Ser Leu Gly Gly Ser Ser Leu Ile Leu Pro Thr Gly
450 455 460
Trp Thr Asn Glu Pro Ile Phe Pro Ser Arg Thr Val Thr Thr Ser Thr
465 470 475 480
Thr Ala Thr Ser Thr Gly Thr Asn Thr Gly Ser Gly Glu Asp Thr Glu
485 490 495
Ala Thr Asp Ser Asp Gln Gly Thr Ser Gly Gly Thr Gly Thr Gln Ala
500 505 510
Glu Gly Pro Val Ala Thr Asp Gly Ala Val Gly Val Gly Asn Ser Pro
515 520 525
Leu Leu Ala Ile Ala Met Ala Ala Leu Val Leu Asn Met Val Leu Ala
530 535 540
Leu
545
<210> 505
<211> 410
<212> PRT
<213> 黑曲霉
<400> 505
Met Lys Ser Thr Thr Leu Leu Ser Leu Ala Trp Ala Thr Gln Ser Ala
1 5 10 15
Tyr Ser Leu Ser Ile His Glu Arg Asp Glu Pro Ala Thr Leu Gln Phe
20 25 30
Asn Phe Glu Arg Arg Gln Ile Ala Asp Arg Ser Arg Arg Lys Arg Ser
35 40 45
Thr Ala Ser Ala Asp Leu Val Asn Leu Ala Thr Asn Leu Gly Tyr Thr
50 55 60
Met Asn Leu Thr Leu Gly Thr Pro Gly Gln Glu Val Ser Val Thr Leu
65 70 75 80
Asp Thr Gly Ser Ser Asp Leu Trp Val Asn Gly Ala Asn Ser Ser Val
85 90 95
Cys Pro Cys Thr Asp Tyr Gly Ser Tyr Asn Ser Ser Ala Ser Ser Thr
100 105 110
Tyr Thr Phe Val Asn Asp Glu Phe Tyr Ile Gln Tyr Val Asp Gly Ser
115 120 125
Glu Ala Thr Gly Asp Tyr Val Asn Asp Thr Leu Lys Phe Ser Asn Val
130 135 140
Thr Leu Thr Asn Phe Gln Phe Ala Val Ala Tyr Asp Gly Asp Ser Glu
145 150 155 160
Glu Gly Val Leu Gly Ile Gly Tyr Ala Ser Asn Glu Ala Ser Gln Ala
165 170 175
Thr Val Gly Gly Gly Glu Tyr Thr Asn Phe Pro Glu Ala Leu Val Asp
180 185 190
Gln Gly Ala Ile Asn Trp Pro Ala Tyr Ser Leu Trp Leu Asp Asp Leu
195 200 205
Asp Glu Gly Lys Gly Thr Ile Leu Phe Gly Gly Val Asn Thr Ala Lys
210 215 220
Tyr Tyr Gly Ser Leu Gln Thr Leu Pro Ile Val Ser Ile Glu Asp Met
225 230 235 240
Tyr Val Glu Phe Ala Val Asn Leu Thr Ala Val His Leu Glu Lys Asn
245 250 255
Gly Asn Ser Val Ser Val Asn Asn Ser Ala Thr Gln Phe Pro Ile Pro
260 265 270
Ala Val Leu Asp Ser Gly Thr Ala Leu Thr Tyr Ile Pro Thr Ser Ala
275 280 285
Ala Ala Ser Ile Tyr Glu Ala Val Gly Ala Gln Tyr Leu Ser Glu Tyr
290 295 300
Gly Tyr Gly Val Ile Glu Cys Gly Val Lys Asp Glu Asp Phe Thr Phe
305 310 315 320
Leu Phe Asp Phe Gly Ser Phe Asn Met Ser Val Asp Ile Ser Glu Met
325 330 335
Ile Leu Glu Ala Ser Ser Asp Met Thr Asp Met Asn Val Cys Thr Phe
340 345 350
Gly Leu Ala Val Ile Glu Asn Glu Ala Leu Leu Gly Asp Thr Phe Leu
355 360 365
Arg Ser Ala Tyr Val Val Tyr Asp Leu Gly Asn Asn Glu Ile Ser Leu
370 375 380
Ala Lys Ala Asn Phe Asn Pro Gly Glu Asp His Val Leu Glu Ile Gly
385 390 395 400
Thr Gly Ser Asp Ala Val Pro Lys Ala Thr
405 410
<210> 506
<211> 486
<212> PRT
<213> 米曲霉
<400> 506
Met Met Arg Gly Ala Ser Leu Leu Pro Val Ala Leu Ala Ala Leu Ser
1 5 10 15
Cys Val Asp Ala Leu Ser Leu His Arg Arg Asp Thr Pro Ala Thr Val
20 25 30
Glu Leu Pro Ile Glu Arg Arg Gln His Ala Gly Gly Leu Gln Lys Arg
35 40 45
Asp Ser Thr Leu Asn Leu Pro Leu Ile Asn Tyr Tyr Asp Ser Phe Tyr
50 55 60
Ile Leu Asn Leu Thr Leu Gly Thr Pro Ala Gln Gln Phe Ala Val Ala
65 70 75 80
Leu Asp Thr Gly Ser Ser Asp Leu Trp Val Asn Val Ala Asn Ser Ser
85 90 95
Tyr Cys Ser Ser Arg Thr Asn Pro Cys Lys Pro Phe Gly Leu Tyr Asp
100 105 110
Pro Asp Ala Ser Ser Thr Tyr Lys Asn Leu Gly Val Glu Phe Asn Ala
115 120 125
Thr Tyr Gly Asp Gly Thr Asn Ala Tyr Gly Tyr Tyr Ala Thr Asp Glu
130 135 140
Leu Gly Leu Gly Asp Val Asn Val Asp Asp Met Gln Phe Gly Val Ala
145 150 155 160
Glu Ser Thr Thr Ile Thr Gln Gly Ile Val Gly Val Ala Tyr Asp Thr
165 170 175
Leu Thr Asn Glu Ala Ser His Glu Gly Lys Thr Tyr Val Asn Leu Pro
180 185 190
Gln Ala Leu Val Asn Ser Gly Ala Ile Lys Ser Pro Ala Tyr Ser Leu
195 200 205
Trp Leu Asn Asp Pro Gln Ala Ser Arg Gly Ser Ile Leu Phe Gly Gly
210 215 220
Val Asn Lys Ala Lys Tyr Lys Gly Glu Leu Gln Thr Ile Pro Ile Val
225 230 235 240
Arg Thr Leu Arg Gly Tyr Ser Tyr Leu Ala Val Thr Leu Thr Gly Val
245 250 255
Ser Val Glu Gln Gly Lys Glu Ser Glu Asp Tyr Ser Ser Arg Leu Pro
260 265 270
Ile Val Val Leu Leu Asp Ser Gly Thr Ser Leu Thr Tyr Leu Pro Asp
275 280 285
Ser Leu Val Asp Glu Leu Tyr Lys Thr Phe Asn Ala Thr Phe Leu Glu
290 295 300
Asp Asp Gly Leu Ala Tyr Val Asp Cys Glu Leu Met Lys Lys Asp Tyr
305 310 315 320
Thr Val Asn Phe Asp Phe Ser Gly Ala Thr Ile Ala Val Gly Ile Ser
325 330 335
Glu Leu Val Leu Lys Ala Val Ala Glu Asp Phe Pro Leu Gly Thr Cys
340 345 350
Ala Phe Gly Val Val Pro Ser Gly Asp Ser Gln Asp Ala Met Tyr Ile
355 360 365
Leu Gly Asp Thr Phe Leu Arg Ser Ala Tyr Val Val Tyr Asp Leu Gly
370 375 380
Asn Asn Glu Ile Ser Leu Ala Asn Thr Asn Phe Ser Pro Gly Asp Asp
385 390 395 400
Asp Ile Leu Glu Ile Gly Thr Gly Thr Ser Ala Val Pro Gly Ala Thr
405 410 415
Pro Val Glu Ser Pro Val Thr Ser Ala Thr Val Ala Ser Ala Thr Asp
420 425 430
Ile Val His Thr Val Met Val Gly Gly Thr Lys Ala Thr Ala Thr Asp
435 440 445
Ser Asn Ser Gly Ala Ala Glu Thr Ser Ser Ser Ser Gly Ile Ala Ala
450 455 460
Leu Pro Thr Ser Asn Thr Arg His Leu Leu Ser Gly Leu Ala Gly Ala
465 470 475 480
Gly Leu Leu Leu Ala Leu
485
<210> 507
<211> 488
<212> PRT
<213> 里氏木霉
<400> 507
Met Arg Ala Ser Pro Leu Ala Val Ala Gly Val Ala Leu Ala Ser Ala
1 5 10 15
Ala Gln Ala Gln Val Val Gln Phe Asp Ile Glu Lys Arg His Ala Pro
20 25 30
Arg Leu Ser Arg Arg Asp Gly Thr Ile Asp Gly Thr Leu Ser Asn Gln
35 40 45
Arg Val Gln Gly Gly Tyr Phe Ile Asn Val Gln Val Gly Ser Pro Gly
50 55 60
Gln Asn Ile Thr Leu Gln Leu Asp Thr Gly Ser Ser Asp Val Trp Val
65 70 75 80
Pro Ser Ser Thr Ala Ala Ile Cys Thr Gln Val Ser Glu Arg Asn Pro
85 90 95
Gly Cys Gln Phe Gly Ser Phe Asn Pro Asp Asp Ser Asp Thr Phe Asp
100 105 110
Glu Val Gly Gln Gly Leu Phe Asp Ile Thr Tyr Val Asp Gly Ser Ser
115 120 125
Ser Lys Gly Asp Tyr Phe Gln Asp Asn Phe Gln Ile Asn Gly Val Thr
130 135 140
Val Lys Asn Leu Thr Met Gly Leu Gly Leu Ser Ser Ser Ile Pro Asn
145 150 155 160
Gly Leu Ile Gly Val Gly Tyr Met Asn Asp Glu Ala Ser Val Ser Thr
165 170 175
Thr Arg Ser Thr Tyr Pro Asn Leu Pro Ile Val Leu Gln Gln Gln Lys
180 185 190
Leu Ile Asn Ser Val Ala Phe Ser Leu Trp Leu Asn Asp Leu Asp Ala
195 200 205
Ser Thr Gly Ser Ile Leu Phe Gly Gly Ile Asp Thr Glu Lys Tyr His
210 215 220
Gly Asp Leu Thr Ser Ile Asp Ile Ile Ser Pro Asn Gly Gly Lys Thr
225 230 235 240
Phe Thr Glu Phe Ala Val Asn Leu Tyr Ser Val Gln Ala Thr Ser Pro
245 250 255
Ser Gly Thr Asp Thr Leu Ser Thr Ser Glu Asp Thr Leu Ile Ala Val
260 265 270
Leu Asp Ser Gly Thr Thr Leu Thr Tyr Leu Pro Gln Asp Met Ala Glu
275 280 285
Glu Ala Trp Asn Glu Val Gly Ala Glu Tyr Ser Asn Glu Leu Gly Leu
290 295 300
Ala Val Val Pro Cys Ser Val Gly Asn Thr Asn Gly Phe Phe Ser Phe
305 310 315 320
Thr Phe Ala Gly Thr Asp Gly Pro Thr Ile Asn Val Thr Leu Ser Glu
325 330 335
Leu Val Leu Asp Leu Phe Ser Gly Gly Pro Ala Pro Arg Phe Ser Ser
340 345 350
Gly Pro Asn Lys Gly Gln Ser Ile Cys Glu Phe Gly Ile Gln Asn Gly
355 360 365
Thr Gly Ser Pro Phe Leu Leu Gly Asp Thr Phe Leu Arg Ser Ala Phe
370 375 380
Val Val Tyr Asp Leu Val Asn Asn Gln Ile Ala Ile Ala Pro Thr Asn
385 390 395 400
Phe Asn Ser Thr Arg Thr Asn Val Val Ala Phe Ala Ser Ser Gly Ala
405 410 415
Pro Ile Pro Ser Ala Thr Ala Ala Pro Asn Gln Ser Arg Thr Gly His
420 425 430
Ser Ser Ser Thr His Ser Gly Leu Ser Ala Ala Ser Gly Phe His Asp
435 440 445
Gly Asp Asp Glu Asn Ala Gly Ser Leu Thr Ser Val Phe Ser Gly Pro
450 455 460
Gly Met Ala Val Val Gly Met Thr Ile Cys Tyr Thr Leu Leu Gly Ser
465 470 475 480
Ala Ile Phe Gly Ile Gly Trp Leu
485
<210> 508
<211> 488
<212> PRT
<213> 绿木霉
<400> 508
Met Lys Ala Ser Pro Leu Ala Val Ala Gly Val Val Leu Ala Ser Ala
1 5 10 15
Ala Gln Ala Gln Val Val Gln Phe Asp Ile Glu Lys Arg His Ala Pro
20 25 30
Ser Leu Arg Arg Arg Asp Ala Thr Ile Asp Ala Thr Leu Ser Asn Glu
35 40 45
Lys Val Gln Gly Gly Tyr Phe Ile Asn Val Gln Val Gly Thr Pro Gly
50 55 60
Gln Asn Ile Thr Leu Gln Leu Asp Thr Gly Ser Ser Asp Val Trp Val
65 70 75 80
Pro Ala Ser Thr Ala Ala Ile Cys Thr Glu Val Ser Gln Gln Asn Pro
85 90 95
Gly Cys Thr Phe Gly Ser Phe Asn Pro Asp Asp Ser Ser Thr Phe Gln
100 105 110
Val Val Gly Gln Asn Leu Phe Asp Ile Thr Tyr Val Asp Gly Ser Ser
115 120 125
Ser Lys Gly Asp Tyr Ile Gln Asp Asn Phe Gln Ile Asn Gly Leu Thr
130 135 140
Val Lys Asn Leu Thr Met Gly Leu Gly Leu Asp Thr Ser Ile Ser Asn
145 150 155 160
Gly Leu Val Gly Val Gly Tyr Ile Asn Asp Glu Ala Ser Leu Gly Thr
165 170 175
Thr Arg Ser Thr Tyr Pro Asn Leu Pro Val Val Leu Gln Gln Glu Lys
180 185 190
Leu Ile Asn Thr Val Ala Phe Ser Leu Trp Leu Asn Asp Leu Asp Ala
195 200 205
Ser Thr Gly Ser Ile Leu Phe Gly Gly Ile Asp Thr Glu Lys Tyr Gln
210 215 220
Gly Glu Leu Thr Arg Ile Asn Ile Ile Ser Pro Asp Gly Gly Lys Thr
225 230 235 240
Phe Thr Glu Phe Ala Val Asn Leu Tyr Ser Val Gln Ala Ser Ser Gln
245 250 255
Ser Gly Thr Asp Thr Leu Ser Thr Ser Glu Asn Thr Leu Val Ala Val
260 265 270
Leu Asp Ser Gly Thr Thr Leu Thr Tyr Leu Pro Gln Asp Met Ala Glu
275 280 285
Glu Ala Trp Asn Glu Val Gly Ala Thr Phe Ser Ser Glu Leu Gly Leu
290 295 300
Ala Val Val Pro Cys Ser Val Gly Asn Lys Asn Gly His Phe Ser Phe
305 310 315 320
Lys Phe Ala Gly Ala Asp Gly Pro Thr Val Asn Val Thr Leu Ser Glu
325 330 335
Leu Val Leu Asp Leu Phe Ser Gly Gly Pro Pro Pro Gln Phe Ser Ser
340 345 350
Gly Pro Asn Lys Gly Gln Ser Ile Cys Glu Phe Gly Ile Gln Asn Thr
355 360 365
Thr Asp Ala Pro Tyr Leu Leu Gly Asp Thr Phe Leu Arg Ser Ala Phe
370 375 380
Val Thr Tyr Asp Leu Val Asn Asn Gln Ile Gly Ile Ala Pro Thr Asn
385 390 395 400
Phe Asn Ser Thr Gln Thr Asn Ile Val Ala Phe Ala Ser Ser Gly Ala
405 410 415
Pro Ile Pro Ser Ser Thr Ala Ala Pro Asn Gln Ser Asn Thr Gly His
420 425 430
Ser Ser Ser Thr Gln Ser Gly Leu Ser Ala Ala Ser Gly Phe Gln Asp
435 440 445
Gly Gly Asn Asp Asn Ala Ala Pro Leu Thr Ser Ala Phe Ser Gly Pro
450 455 460
Gly Met Phe Val Val Gly Met Thr Ile Cys Tyr Thr Leu Leu Gly Ser
465 470 475 480
Ala Val Phe Ser Phe Arg Trp Leu
485
<210> 509
<211> 488
<212> PRT
<213> Trichoderma atraviride
<400> 509
Met Lys Ala Ser Pro Leu Ala Ala Ala Gly Val Ala Leu Ala Ser Thr
1 5 10 15
Ala Gln Ala Gln Val Val Gln Phe Asp Ile Gln Lys Arg Asp Gly Pro
20 25 30
Asn Leu Arg Asp Leu Gly Arg Arg Ala Ala Thr Ile Asn Gly Val Leu
35 40 45
Ser Asn Gln Gln Ile Gln Gly Gly Tyr Phe Leu Asn Val Glu Val Gly
50 55 60
Thr Pro Pro Gln Asn Ile Thr Leu Gln Leu Asp Thr Gly Ser Ser Asp
65 70 75 80
Val Trp Val Pro Ser Ser Ser Ala Asp Ile Cys Thr Gln Val Ser Gln
85 90 95
Arg Asn Pro Gly Cys Thr Phe Gly Ser Phe Asp Ser Asp Lys Ser Thr
100 105 110
Ser Leu Arg Thr Val Gly Lys Asp Glu Phe Asp Ile Thr Tyr Val Asp
115 120 125
Asn Ser Phe Ser Lys Gly Asp Tyr Ile Gln Asp Asp Phe His Ile Gly
130 135 140
Gly Val Thr Val Ser Asn Leu Thr Met Gly Leu Gly Leu Asp Ser Ser
145 150 155 160
Ile Ala Asn Gly Leu Ile Gly Val Gly Tyr Ile Asn Asp Glu Ala Ser
165 170 175
Leu Ser Thr Ile Arg Ser Thr Tyr Pro Asn Leu Pro Val Val Leu Gln
180 185 190
Gln Glu Lys Leu Ile Asn Thr Val Ala Tyr Ser Leu Trp Leu Asn Asp
195 200 205
Leu Asp Ala Ser Ala Gly Ser Ile Leu Phe Gly Gly Ile Asp Thr Glu
210 215 220
Lys Tyr Gln Gly Asp Leu Thr Lys Ile Asn Ile Gln Arg Ala Asp Asn
225 230 235 240
Ser Asn Val Phe Thr Glu Phe Ala Val Glu Leu Phe Glu Val Gln Ala
245 250 255
Thr Ser Pro Ser Gly Thr Asp Thr Leu Ser Thr Ser Val Asp Ser Leu
260 265 270
Val Ala Val Leu Asp Ser Gly Thr Thr Leu Thr Tyr Leu Pro Gln Asp
275 280 285
Met Ala Glu Gln Ala Trp Lys Glu Val Gly Ala Ser Phe Asn Glu Glu
290 295 300
Phe Gly Leu Ala Val Val Pro Cys Ser Ile Arg Asn Val Asn Gly His
305 310 315 320
Phe Ser Phe Thr Phe Ala Gly Ala Asp Gly Pro Lys Ile Asn Val Thr
325 330 335
Met Ala Glu Leu Ala Leu Asp Leu Phe Ser Gly Gly Pro Gln Pro Lys
340 345 350
Phe Ser Ser Gly Pro Asn Gln Gly Gln Ser Val Cys Glu Phe Gly Ile
355 360 365
Gln Asn Thr Thr Gly Ser Pro Phe Leu Leu Gly Asp Thr Phe Leu Arg
370 375 380
Ser Ala Phe Val Val Tyr Asp Leu Val Asn Asn Glu Ile Ala Ile Ala
385 390 395 400
Pro Thr Asn Phe Asn Ser Thr Lys Thr Asn Val Val Ala Phe Ala Ser
405 410 415
Ser Gly Ala Pro Ile Pro Ser Ala Thr Ser Ala Pro Asn Gln Ser Lys
420 425 430
Ser Gly Pro Ser Ser Gly Thr Gly Gly Met Ser Ala Ala Ser Gly Phe
435 440 445
Gln Asp Ser Asp Asn Ala Ala Pro Tyr Thr Ser Ala Phe Ser Gly Pro
450 455 460
Gly Ala Ile Ile Val Gly Met Thr Ile Gly Tyr Thr Leu Leu Gly Gly
465 470 475 480
Ala Ile Phe Gly Ala Gly Trp Leu
485
<210> 510
<211> 504
<212> PRT
<213> 四孢链孢菌
<400> 510
Met Lys Gly Tyr Thr Ser Ser Ala Leu Leu Leu Gly Pro Ala Leu Leu
1 5 10 15
Ser Gln Leu Ala Leu Ala Gln Gln Ala Pro Ser Gly Val Val His Trp
20 25 30
Gly Ile Gln Lys Arg His Ala Pro Asn Ala Pro Asn Arg Leu Leu Arg
35 40 45
Arg Ala Gly Pro Thr His Gln Ala Ile Leu Gln Asn Glu Gln Ala Arg
50 55 60
Gly Gly Tyr Phe Ala Thr Cys Glu Met Gly Thr Pro Gly Gln Lys Val
65 70 75 80
Thr Leu Gln Leu Asp Thr Gly Ser Ser Asp Val Trp Val Pro Asp Ser
85 90 95
Thr Ala Ser Ile Cys Asn Lys Gly Ala Cys Asp Leu Gly Ser Phe Asp
100 105 110
Pro Ser Lys Ser Glu Thr Tyr Thr Val Val Gly Gln Gly Glu Phe Asp
115 120 125
Ile Ser Tyr Val Asp Gly Ser Ser Ser Lys Gly Asp Tyr Phe Thr Asp
130 135 140
Val Phe Asn Ile Gly Gly Thr Thr Val Thr Asn Leu Thr Met Gly Leu
145 150 155 160
Gly Ala Gln Thr Asp Ile Ala Tyr Gly Leu Val Gly Ile Gly Tyr Ala
165 170 175
Ile Asn Glu Ala Ile Val Gly Asn Ser His Ser Leu Ser Ser Gln Tyr
180 185 190
Pro Asn Leu Pro Val Ala Met Val Asp Asp Gly Leu Ile Asn Thr Ile
195 200 205
Ala Tyr Ser Leu Trp Leu Asn Asp Leu Asp Ala Asp Glu Gly Ser Ile
210 215 220
Leu Phe Gly Gly Ile Asp Thr Lys Lys Tyr Lys Gly Asp Leu Thr Arg
225 230 235 240
Ile Arg Ile Tyr Pro Ser Ser Asn Gly Tyr Tyr Phe Ser Phe Ile Val
245 250 255
Ala Leu Thr Ser Leu Gln Ala Ile Ser Pro Ser Gly Asn Asp Thr Leu
260 265 270
Thr Ser Gln Glu Phe Pro Ile Pro Val Val Leu Asp Ser Gly Thr Thr
275 280 285
Leu Ser Tyr Leu Pro Gln Asp Ile Val Asp Gln Ile Trp Gln Glu Val
290 295 300
Gly Ala Glu Tyr Ser Asp Arg Leu Glu Leu Ala Val Ile Pro Cys Ser
305 310 315 320
Lys Lys Ser Ser Asn Gly Tyr Phe Ser Phe Gly Phe Ala Gly Pro Asp
325 330 335
Gly Pro Arg Ile Thr Val Arg Met Asp Glu Leu Val Leu Asp Leu Thr
340 345 350
Ser Gly Asp Pro Pro Lys Tyr Thr Ser Gly Pro Asn Lys Gly Gln Asp
355 360 365
Val Cys Glu Phe Gly Ile Gln Asn Ser Thr Ser Ala Pro Tyr Leu Leu
370 375 380
Gly Asp Thr Phe Leu Arg Ser Ala Tyr Val Val Tyr Asp Leu Val Asn
385 390 395 400
Asn Glu Ile Ala Leu Ala Glu Thr Asp Phe Asn Ser Thr Glu Ser Asn
405 410 415
Ile Val Ala Phe Ala Ser Met Ser Ala Thr Ile Pro Ser Ala Thr Gln
420 425 430
Ala Pro Asn Gln Ala Ala Val Thr Asn Arg Pro Val Ala Thr Met Pro
435 440 445
Ser Phe Ala Ala Ser Ser Gly Phe Ser Asp Thr Gly Gly Ser Gly Asn
450 455 460
Asp Gly Lys Asp Glu Asn Ala Ser Ala Gly Met Pro Ser Ala Phe Gly
465 470 475 480
Val Ala Gln Met Ser Val Met Gly Ile Ala Met Val Phe Ala Met Ile
485 490 495
Gly Ser Gly Val Phe Val Leu Leu
500
<210> 511
<211> 501
<212> PRT
<213> 嗜热毁丝霉
<400> 511
Met Arg Gly Tyr Ala Ala Val Ala Phe Gly Ala Ile Leu Ala Gly Ala
1 5 10 15
Val His Ala Ser Ala Gly Asn Gly Val Val Gln Trp Asp Ile Arg Arg
20 25 30
Thr Gln Arg Gln Glu Glu Leu Gln Arg Leu Asn Arg Arg Leu Arg Lys
35 40 45
Arg Ala Asn Pro Val Leu Glu Val Ile Thr Asn Glu Lys Ile Arg Gly
50 55 60
Gly Tyr Phe Ala Thr Cys Lys Ile Gly Thr Pro Gly Gln Asp Leu Thr
65 70 75 80
Leu Gln Leu Asp Thr Gly Ser Ser Asp Ile Trp Val Pro Asp Ser Ala
85 90 95
Ala Gln Val Cys Arg Glu Ile Gly Thr Glu Gly Cys Ala Leu Gly Thr
100 105 110
Phe Asn Pro Asn Arg Ser Ser Ser Phe Glu Val Ile Gly Glu Gly Gln
115 120 125
Phe Asp Ile Glu Tyr Val Asp Gly Ser Ser Ser Lys Gly Asp Tyr Phe
130 135 140
Thr Asp Val Phe Gln Ile Gly Asp Ile Ser Val Gln Asn Met Thr Met
145 150 155 160
Gly Leu Gly Leu His Thr Asp Ile Ala Tyr Gly Leu Val Gly Val Gly
165 170 175
Tyr Ala Ile Asn Glu Ala Ile Val Ala Thr Thr Gln Ser Arg Asp Ser
180 185 190
Val Tyr Pro Asn Leu Pro Val Gln Met Val Asp Gln Gly Leu Ile Asn
195 200 205
Thr Val Ala Tyr Ser Leu Trp Leu Asn Asp Leu Asp Ala Ser Ser Gly
210 215 220
Ser Ile Leu Phe Gly Gly Ile Asp Thr Glu Lys Tyr Gln Gly Glu Leu
225 230 235 240
Thr Arg Ile Asp Ile Tyr Pro Thr Ser Gln Gly Asp Phe Ser Ser Phe
245 250 255
Val Val Ala Leu Thr Ser Leu Glu Ala Arg Ser Pro Ser Gly Gln Asp
260 265 270
Thr Leu Thr Ser Gln Glu Phe Pro Ile Pro Val Val Leu Asp Ser Gly
275 280 285
Thr Thr Leu Ser Tyr Leu Pro Thr Asp Leu Ala Thr Gln Ala Trp Lys
290 295 300
Glu Val Gly Ala Phe Tyr Leu Pro Glu Val Gly Ala Ala Val Leu Pro
305 310 315 320
Cys Asp Met Glu Asn Ser Lys Gly Ser Phe Ser Phe Gly Phe Ala Gly
325 330 335
Pro Asp Gly Pro Arg Ile Thr Val Gly Met Asp Glu Leu Val Leu Asp
340 345 350
Met Thr Asp Gly Gln Ala Pro Gln Phe Leu Ser Gly Pro Tyr Lys Gly
355 360 365
Arg Asp Val Cys Gln Phe Gly Ile Gln Asn Phe Thr Ser Ala Pro Phe
370 375 380
Leu Leu Gly Asp Thr Phe Leu Arg Ser Ala Tyr Val Val Tyr Asp Leu
385 390 395 400
Val Asn Asn Gln Ile Gly Ile Ala Ala Thr Asp Phe Asn Ser Thr Asp
405 410 415
Ser Asn Ile Val Pro Phe Pro Ser Met Gly Ala Pro Ile Pro Ser Ala
420 425 430
Thr Val Ala Ala Asn Gln Arg Glu Val Thr Arg Val Pro Thr Val Thr
435 440 445
Glu Pro Ala Tyr Ser Ala Ser Gln Gly Phe Met Glu Ser Ala Ser Gly
450 455 460
Glu Glu Ser Leu Ala Pro Gly Met Pro Ala Ala Trp Gly Met Gly Gln
465 470 475 480
Leu Leu Val Val Gly Val Thr Met Ala Leu Thr Ala Leu Gly Ser Gly
485 490 495
Leu Phe Phe Val Leu
500
<210> 512
<211> 492
<212> PRT
<213> 粗糙链孢霉
<400> 512
Met Lys Gly Tyr Thr Ser Ser Ala Leu Leu Leu Gly Pro Ala Leu Leu
1 5 10 15
Ser Gln Leu Ala Leu Ala Gln Gln Ala Pro Asn Gly Val Val His Trp
20 25 30
Gly Ile Gln Lys Arg His Ala Pro Asn Ala Pro Asn Arg Leu Leu Arg
35 40 45
Arg Ala Gly Pro Thr His Gln Ala Ile Leu Gln Asn Glu Gln Ala Arg
50 55 60
Gly Gly Tyr Phe Ala Thr Cys Ala Met Gly Thr Pro Gly Gln Lys Val
65 70 75 80
Thr Leu Gln Leu Asp Thr Gly Ser Ser Asp Val Trp Val Pro Asp Ser
85 90 95
Thr Ala Ser Ile Cys Asn Lys Gly Ala Cys Asp Leu Gly Ser Trp Gln
100 105 110
Gly Glu Phe Asp Ile Ser Tyr Val Asp Gly Ser Ser Ser Lys Gly Asp
115 120 125
Tyr Phe Thr Asp Val Phe Asn Ile Gly Gly Thr Thr Val Thr Asn Leu
130 135 140
Thr Met Gly Leu Gly Ala Gln Thr Asp Ile Ala Tyr Gly Leu Val Gly
145 150 155 160
Ile Gly Tyr Ala Ile Asn Glu Ala Ile Val Gly Asn Ser His Ser Leu
165 170 175
Ser Ser Gln Tyr Pro Asn Leu Pro Val Ala Met Val Asp Asp Gly Leu
180 185 190
Ile Asn Thr Ile Ala Tyr Ser Leu Trp Leu Asn Asp Leu Asp Ala Gly
195 200 205
Glu Gly Ser Ile Leu Phe Gly Gly Ile Asp Thr Lys Lys Tyr Lys Gly
210 215 220
Asp Leu Thr Arg Ile Arg Ile Tyr Pro Ser Ser Asn Gly Tyr Tyr Phe
225 230 235 240
Ser Phe Ile Val Ala Leu Thr Ser Leu Gln Ala Ile Ser Pro Ser Gly
245 250 255
Asn Asp Thr Leu Thr Ser Gln Glu Phe Pro Ile Pro Val Val Leu Asp
260 265 270
Ser Gly Thr Thr Leu Ser Tyr Leu Pro Gln Asp Ile Val Asp Gln Ile
275 280 285
Trp Gln Glu Val Gly Ala Glu Tyr Ser Asp Arg Leu Glu Leu Ala Val
290 295 300
Ile Pro Cys Ser Lys Lys Ser Ser Asn Gly Tyr Phe Ser Phe Gly Phe
305 310 315 320
Ala Gly Pro Asp Gly Pro Arg Ile Thr Val Arg Met Asp Glu Leu Val
325 330 335
Leu Asp Leu Thr Ser Gly Asp Pro Pro Lys Tyr Thr Ser Gly Pro Asn
340 345 350
Lys Gly Gln Asp Val Cys Glu Phe Gly Ile Gln Asn Ser Thr Ser Ala
355 360 365
Pro Tyr Leu Leu Gly Asp Thr Phe Leu Arg Ser Ala Tyr Val Val Tyr
370 375 380
Asp Leu Val Asn Asn Glu Ile Gly Leu Ala Glu Thr Asp Phe Asn Ser
385 390 395 400
Thr Glu Ser Asn Ile Val Ala Phe Ala Ser Met Ser Ala Thr Ile Pro
405 410 415
Ser Ala Thr Gln Ala Pro Asn Gln Ala Ala Val Thr Asn Arg Pro Val
420 425 430
Ala Thr Met Pro Ser Phe Ala Ala Ser Ser Gly Phe Ser Asp Thr Gly
435 440 445
Gly Ser Gly Asn Asp Gly Lys Asp Glu Asn Ala Ser Ala Gly Met Pro
450 455 460
Ser Ala Phe Gly Val Ala Gln Met Ser Val Met Gly Ile Ala Met Val
465 470 475 480
Phe Ala Met Val Gly Ser Gly Val Phe Val Leu Leu
485 490
<210> 513
<211> 484
<212> PRT
<213> 金龟子绿僵菌
<400> 513
Met Lys Tyr Ser Ser Ala Val Asp Ala Cys Val Ser Ser Val Ile Ile
1 5 10 15
Leu Ala Pro Ala Val Phe Gly Gln Val Val Arg Trp Asp Ile Gln Lys
20 25 30
His Tyr Gln Ala Ser Gly Leu Arg Arg Arg Ala Gly Thr Leu Gln Ser
35 40 45
Asp Ala Ser Asn Asn Lys Ala Gln Gly Gly Tyr Phe Val Ala Val Gln
50 55 60
Ile Gly Thr Pro Gly Gln Glu Leu Ser Leu Gln Leu Asp Thr Gly Ser
65 70 75 80
Ser Asp Val Trp Val Pro Ser Ser Gln Ala Pro Val Cys Leu Asp Lys
85 90 95
Ala Gln Gly Gly Cys Pro Leu Gly Ser Phe Asp Phe Asp Gln Ser Gly
100 105 110
Thr Phe Asp Val Ile Val Ala Asn Gly Phe Asp Ile Ser Tyr Gln Asp
115 120 125
Asn Ser Ser Ala Ser Gly Asp Tyr Phe Ala Asp Lys Leu Glu Ile Gly
130 135 140
Ser Lys Ser Leu Asn Asn Phe Thr Met Gly Leu Gly Lys Lys Thr Asp
145 150 155 160
Val Pro Phe Gly Leu Ile Gly Val Gly Tyr Ala Gly Asn Glu Ala Ser
165 170 175
Thr Glu Thr Ala Asn Ala Ile Tyr Pro Asn Leu Pro Ile Ala Met Gln
180 185 190
Glu Ala Gly Ile Ile Asn Ser Val Ala Tyr Ser Leu Trp Leu Asn Asp
195 200 205
Leu Gly Ala Ser Thr Gly Asn Ile Leu Phe Gly Gly Ile Asp Thr Glu
210 215 220
Lys Tyr Val Gly His Leu Thr Lys Leu Pro Ile Gln Pro Glu Asn Gln
225 230 235 240
Gln Phe Thr His Phe Ile Val Ser Leu Phe Ser Leu Glu Ala Thr Ser
245 250 255
Pro Ser Gly Asn Asp Val Leu Thr Ser Arg Glu Leu Pro Leu Lys Val
260 265 270
Val Leu Asp Ser Gly Thr Thr Leu Ser Tyr Leu Pro Pro Asp Met Ala
275 280 285
Ala Gln Val Trp Ser Glu Val Gly Ala Thr Tyr Asp Asp Met Ser Phe
290 295 300
Gln Thr Ala Ile Ile Pro Cys Ser Tyr Ala Ser His Ala Gly His Phe
305 310 315 320
Ser Phe Val Phe Ser Gly Pro Asp Gly Pro Arg Ile Asn Val Thr Met
325 330 335
Asp Glu Leu Val Leu Pro Leu Thr Asp Gly Glu Pro Pro Thr Phe Ser
340 345 350
Ser Gly Pro His Ser Gly Gln Thr Ile Cys Glu Phe Gly Ile Gln Lys
355 360 365
Ser Ser Asp Thr Pro Trp Leu Leu Gly Asp Thr Phe Leu Arg Ser Ala
370 375 380
Tyr Val Val Tyr Asp Leu Met Asn Asn Glu Val Gly Ile Ala Pro Thr
385 390 395 400
Asp Phe Asn Ser Thr Lys Ser Asn Val Ile Ala Phe Ala Ser Lys Gly
405 410 415
Ala Ala Ile Pro Ser Ala Thr Ser Val Asn Asn Gly Ser Lys Ser Gly
420 425 430
Ile Gln Ser Gln Pro Thr Gln Thr Gly Leu Ser Ala Ala Lys Gly Phe
435 440 445
Gln Glu Gly Ser Leu Ala Cys Ser Leu Glu Ser Leu Ser Val Phe Ser
450 455 460
Leu Thr Phe Ile Ser Met Thr Ile Thr Ala Leu Leu Met Gly Ser Pro
465 470 475 480
Ile Phe Thr Asp
<210> 514
<211> 507
<212> PRT
<213> 太瑞斯梭孢壳霉
<400> 514
Met Lys Gly His Ser Ala Ala Ala Leu Gly Ala Leu Leu Val Gly Val
1 5 10 15
Val His Pro Val Ala Ala Asn Gly Val Val Gln Trp Asp Ile Arg Arg
20 25 30
Ser Gln Arg Ser Gln Glu Phe Thr Lys Leu Arg Arg Arg Gly Ser Thr
35 40 45
Phe Asn Glu Val Ile Ser Asn Asp Glu Ala Arg Gly Gly Tyr Phe Ala
50 55 60
Thr Cys Gln Ile Gly Thr Pro Gly Gln Asn Leu Thr Leu Gln Leu Asp
65 70 75 80
Thr Gly Ser Ser Asp Ile Trp Val Pro Asp Ser Lys Ala Ser Val Cys
85 90 95
Arg Lys Phe Gly Asn Ser Gly Gly Cys Asn Leu Gly Ala Phe Asp Ser
100 105 110
Ala Gln Ser Ser Ser Phe Glu Val Val Gly Lys Gly Glu Phe Asp Ile
115 120 125
Ser Tyr Val Asp Gly Ser Ser Ser Lys Gly Asp Tyr Phe Thr Asp Val
130 135 140
Phe Ser Ile Gly Gly Ala Thr Leu Gln Asn Met Thr Met Gly Leu Gly
145 150 155 160
Leu Glu Thr Asp Ile Ser Tyr Gly Leu Val Gly Val Gly Tyr Ala Val
165 170 175
Asn Glu Ala Ile Val Gly Ser Ser Gln Ser Val Ser Ser Ala Tyr Pro
180 185 190
Asn Leu Pro Val Asn Met Val Asn Glu Gly Leu Ile Asn Thr Val Ala
195 200 205
Tyr Ser Leu Trp Leu Asn Asp Leu Asp Ala Ser Ser Gly Ser Ile Leu
210 215 220
Phe Gly Gly Ile Asp Thr Lys Lys Tyr Lys Gly Asp Leu Thr Arg Ile
225 230 235 240
Gln Ile Tyr Pro Ala Thr Gln Asp Ile Phe Thr Ser Phe Lys Val Ala
245 250 255
Leu Thr Ser Leu Glu Ala Ile Ser Pro Ser Gly Gln Asp Thr Leu Thr
260 265 270
Ser Thr Ala Phe Pro Ile Ala Val Val Leu Asp Ser Gly Thr Thr Leu
275 280 285
Ser Tyr Leu Pro Thr Asp Leu Ala Met Gln Ile Trp Lys Glu Ala Gly
290 295 300
Ala Glu Tyr Ser Ala His Tyr Gly Thr Ala Ile Ile Pro Cys Ser Met
305 310 315 320
Gln Asn Ser Gln Gly Tyr Phe Ser Phe Gly Phe Ala Gly Pro Gly Gly
325 330 335
Pro Arg Ile Asn Val Thr Met Asp Glu Leu Val Leu Asp Val Ser Thr
340 345 350
Asp Gly Gly Pro Gln Phe Thr Ser Gly Gln Tyr Lys Gly Glu Thr Ile
355 360 365
Cys Glu Phe Gly Ile Gln Asn Ile Ser Ser Ser Pro Tyr Leu Leu Gly
370 375 380
Asp Thr Phe Leu Arg Ser Ala Tyr Val Val Tyr Asp Leu Val Asn Asn
385 390 395 400
Glu Val Gly Ile Ala Gln Thr Asp Phe Asn Ser Thr Glu Ser Asn Val
405 410 415
Val Ala Phe Ala Ser Met Ser Ala Thr Ile Pro Ser Ala Thr Val Ala
420 425 430
Pro Ser Gln Ala Glu Val Thr Asn Arg Pro Thr Val Thr Val Pro Ala
435 440 445
Tyr Ala Ala Ser Lys Gly Phe Thr Asp Leu Ser Ala Ser Gly Thr Ala
450 455 460
Ala Ser Thr Ser Ala Glu Gln Asn Ala Ala Thr Pro Thr Pro Ser Arg
465 470 475 480
Ala Ser Thr Gly Gln Met Leu Val Val Ser Leu Thr Met Leu Leu Thr
485 490 495
Ala Val Gly Ser Gly Leu Phe Val Ala Ile Leu
500 505
<210> 515
<211> 494
<212> PRT
<213> 尖孢镰刀菌
<400> 515
Met Lys Ser Ser Leu Val Gly Ala Ser Leu Val Ser Thr Leu Gly Leu
1 5 10 15
Val Gln Ala Gln Thr Ile Gln Trp Asn Ile Glu Gly Arg His Pro Gln
20 25 30
Pro His Leu Ala Arg Arg Ala Lys Thr Thr Tyr Glu Glu Val Ile Ser
35 40 45
Asn Asp Arg Ser Glu Gly Gly Tyr Phe Thr Glu Val Thr Ile Gly Asn
50 55 60
Pro Pro Gln Asn Ile Thr Leu Gln Leu Asp Thr Gly Ser Ser Asp Val
65 70 75 80
Trp Val Pro Trp Asn Cys Ala Asp Ile Cys Glu Asp Asp Lys Asn Gly
85 90 95
Lys Ser Gly Cys Pro Leu Gly Ser Phe Asp Pro Asp Lys Ser Lys Thr
100 105 110
Phe Lys His Val Ala Pro Gly Met Phe Gly Ile Thr Phe Val Asp Asp
115 120 125
Ser Tyr Val Lys Gly Asp Tyr Phe Glu Asp His Phe Glu Leu Asp Gly
130 135 140
Ala Val Ile Asn Asn Leu Thr Met Gly Leu Ala Ile Lys Thr Asn Ile
145 150 155 160
Ser Tyr Gly Leu Ile Gly Val Gly Tyr Ala Lys Asn Glu Ala Ser Gly
165 170 175
Ser Thr Thr Asp Val Ile Tyr Pro Asn Leu Pro Val Ala Met Tyr Gln
180 185 190
Ala Gly Tyr Ile Asn Thr Ile Ala Tyr Ser Val Trp Leu Asn Asp Leu
195 200 205
Asp Ala Lys Ala Gly Ser Ile Leu Phe Gly Gly Val Asp Thr Ala Lys
210 215 220
Tyr Val Gly Asp Met His Arg Ile Asp Ile Gln Lys Leu Asp Gly His
225 230 235 240
Tyr Tyr His Phe Val Val Ala Leu Thr Ser Leu Val Ala Thr Ser Ser
245 250 255
Ser Gly Ser Asp Val Leu Thr Ser Asp Ser Phe Pro Ile Gln Ala Val
260 265 270
Leu Asp Ser Gly Thr Thr Leu Ser Tyr Leu Pro Gln Asp Leu Ala His
275 280 285
Glu Ile Trp Glu Glu Val Gly Ala Val Trp Ser Pro Tyr Tyr Gly Val
290 295 300
Ala Val Leu Pro Cys Ala Tyr Gly Arg Asn Gln Gly Asn Phe Thr Phe
305 310 315 320
Gly Phe Ala Gly Pro Asp Gly Pro Ser Ile Ser Val Gly Met Asp Glu
325 330 335
Leu Val Leu Ser Met Thr Ser Asp Ala Ala Asn Met Pro Val Phe Glu
340 345 350
Ser Gly Ala Tyr Lys Gly Glu Asn Ile Cys Thr Phe Gly Ile Gln Asn
355 360 365
Asp Thr Asn Asp Leu Tyr Leu Leu Gly Asn Thr Phe Leu Arg Ser Ala
370 375 380
Tyr Val Val Tyr Asp Leu Val Asn Asn Glu Val Gly Ile Ala Ala Thr
385 390 395 400
Asp Phe Asn Ser Thr Lys Ser Lys Arg Val Pro Phe Lys Ser Tyr Gly
405 410 415
Ala Thr Ile Pro Ser Ala Thr Ala Ser Ser Asn Gln His Arg Ala Thr
420 425 430
Glu Lys Pro Thr Val Pro Gly His Asn Phe Thr Ala Ala Glu Gly Phe
435 440 445
Gln Glu Ser Asp Ser Asn Gly Asn Gly Asp Lys Asp Asn Ala Gly Ser
450 455 460
Leu Leu Asn Pro Pro Thr Thr Pro Leu Ala Leu Val Val Thr Thr Met
465 470 475 480
Ser Phe Met Leu Ala Gly Gly Gly Ile Phe Ser Ser Ile Phe
485 490
<210> 516
<211> 492
<212> PRT
<213> 玉米赤霉
<400> 516
Met Lys Ser Trp Leu Trp Gly Ala Ser Leu Val Ser Thr Trp Gly Leu
1 5 10 15
Val Tyr Ala Gln Thr Val Gln Trp Ser Ile Glu Gly Arg His Pro Gln
20 25 30
Pro Gln Leu Ala Arg Arg Thr Lys Thr Thr Tyr Glu Glu Val Ile Ser
35 40 45
Asn Asp Arg Ser Glu Gly Gly Tyr Phe Thr Glu Val Thr Ile Gly Ser
50 55 60
Pro Pro Gln Lys Val Thr Leu Gln Leu Asp Thr Gly Ser Ser Asp Val
65 70 75 80
Trp Val Pro Trp Asn Cys Ala Asp Val Cys Thr Asp Asn Asp Asn Gly
85 90 95
Lys Gly Gly Cys Pro Leu Gly Ser Phe Asp Pro Asp Lys Ser Lys Ser
100 105 110
Phe Lys His Val Ala Pro Gly Met Phe Gly Ile Thr Phe Val Asp Asn
115 120 125
Ser Tyr Val Lys Gly Asp Tyr Phe Glu Asp His Phe Glu Leu Asp Gly
130 135 140
Ala Val Ile Asn Asn Leu Thr Met Gly Leu Ala Val Lys Thr Asn Ile
145 150 155 160
Ser Tyr Gly Leu Ile Gly Val Gly Tyr Ala Lys Asn Glu Ala Ser Gly
165 170 175
Ser Thr Thr Asp Val Ile Tyr Pro Asn Leu Pro Val Ala Met Tyr Glu
180 185 190
Ala Gly Tyr Val Asn Thr Ile Ala Tyr Ser Val Trp Leu Asn Asp Leu
195 200 205
Asp Ala Lys Ala Gly Thr Val Leu Phe Gly Gly Val Asp Thr Ala Lys
210 215 220
Tyr Val Gly Asp Met His Arg Ile Asp Ile Gln Lys Val Asp Gly His
225 230 235 240
Tyr Tyr His Phe Val Val Ala Leu Thr Ser Leu Val Ala Thr Ser Ser
245 250 255
Ser Gly Ser Asp Asn Leu Thr Ser Glu Cys Phe Pro Ile Pro Ala Val
260 265 270
Leu Asp Ser Gly Thr Thr Leu Ser Tyr Leu Pro His Asp Leu Ala Thr
275 280 285
Glu Ile Trp Gln Glu Val Gly Ala Val Trp Ser Pro Tyr Tyr Gly Val
290 295 300
Ala Val Leu Pro Cys Ala Tyr Gly Arg Asn Asn Gly Asn Phe Thr Phe
305 310 315 320
Gly Phe Ala Gly Pro Asp Gly Pro Val Ile Ser Val Gly Met Asp Glu
325 330 335
Leu Val Leu Thr Met Ala Ser Asp Arg Ala Asn Met Pro Val Phe Gln
340 345 350
Ser Gly Pro Tyr Lys Gly His Thr Met Cys Ser Phe Gly Ile Gln Asn
355 360 365
Asp Thr Ser Asp Leu Tyr Leu Leu Gly Asn Thr Phe Leu Arg Ser Ala
370 375 380
Tyr Val Val Tyr Asp Leu Val Asn Asn Glu Val Gly Ile Ala Ala Thr
385 390 395 400
Asp Phe Asn Ser Thr Ala Thr Lys Arg Val Ala Phe Lys Ser Tyr Gly
405 410 415
Ala Ser Ile Pro Ser Ala Thr Ala Val Ala Asn Gln His Lys Ala Thr
420 425 430
Glu Asn Pro Ser Val Ser Gly His Asn Phe Thr Ala Ala Asp Gly Phe
435 440 445
Gln Glu Ser Asp Trp Asp Ser Asp Asp Glu Asp Asn Ala Ala Ile Ser
450 455 460
Leu Thr Pro Ser Gly Thr Pro Leu Val Met Ala Val Val Thr Met Gly
465 470 475 480
Phe Val Leu Leu Gly Gly Gly Ile Phe His Thr Phe
485 490
<210> 517
<211> 484
<212> PRT
<213> 稻瘟病菌
<400> 517
Met Leu Gly Ile Ser Ala Leu Leu Ile Val Cys Ala Ser Leu Gly Ser
1 5 10 15
Phe Ala Ala Ala Ser Asp Gly Val Val Gln Trp His Ile Glu Arg Arg
20 25 30
Gln Val Pro Thr Pro Thr Ala Lys Leu Ala Lys Arg Val Ala Thr Thr
35 40 45
Phe Glu Glu Val Ile Gln Asn Glu Lys Ser Arg Gly Gly Tyr Phe Ala
50 55 60
Lys Cys Ser Val Gly Gly Gln Asp Val Thr Leu Gln Leu Asp Thr Gly
65 70 75 80
Ser Ser Asp Leu Trp Val Pro Ala Glu Ser Ala Cys Gln Ser Asp Arg
85 90 95
Val Pro Asn Gly Cys Ser Leu Gly Ser Phe Lys Lys Asp Leu Ser Ser
100 105 110
Ser Phe Lys Val Thr Gly Lys Asp Gln Phe Ser Ile Gln Tyr Ile Asp
115 120 125
Gly Ser Phe Ala Arg Gly Asp Tyr Phe Thr Asp Ala Phe Ser Ile Gly
130 135 140
Gly Val Arg Val Ile Asn Leu Thr Met Gly Leu Gly Lys Gln Thr Asp
145 150 155 160
Ile Pro Tyr Gly Leu Ala Gly Val Gly Tyr Ala Leu Asn Glu Ala Ile
165 170 175
Val Gly Gly Asn Ser Ser Asn Phe Thr Thr Tyr Pro Asn Leu Pro Ala
180 185 190
Thr Met Leu Asn Asp Gly Leu Ile Asn Thr Asn Ala Phe Ser Leu Trp
195 200 205
Leu Asn Asn Leu Asp Ala Ser Thr Gly Arg Ile Leu Phe Gly Gly Val
210 215 220
Asp Val Ala Lys Phe Lys Gly Gly Met Ala Arg Ile Arg Ile Tyr Glu
225 230 235 240
Asp Val Phe Ser Gln Glu Tyr Met Ser Phe Thr Val Ala Leu Thr Ser
245 250 255
Leu Glu Ala His Ser Gly Ser Gly Gln Asp Thr Leu Thr Ser Arg Ser
260 265 270
Phe Pro Ile Pro Val Val Leu Asp Ser Gly Thr Thr Leu Ser Tyr Leu
275 280 285
Pro Asn Asp Leu Ala Val Gln Thr Trp Thr Glu Val Gly Ala Leu Tyr
290 295 300
Ser Ala Glu Tyr Gly Gln Ala Leu Leu Pro Cys Ala Met Glu Asp Ser
305 310 315 320
Lys Gly Tyr Phe Val Phe Gly Phe Ala Gly Pro Asp Gly Pro Lys Ile
325 330 335
Gln Val Glu Met Lys Glu Leu Val Leu Asp Leu Thr Asn Gly Pro Ala
340 345 350
Pro Lys Phe Thr Asn Gly Lys Tyr Gln Gly Gln Asp Ala Cys Thr Phe
355 360 365
Gly Ile Gln Asn Phe Thr Gly Gln Thr Met Val Leu Gly Asp Thr Phe
370 375 380
Leu Arg Ser Ala Tyr Val Val Tyr Asp Leu Val Asn His Glu Ile Gly
385 390 395 400
Ile Ala Gln Thr Asp Phe Asn Ser Thr Thr Ser Glu Ile Ile Ala Phe
405 410 415
Pro Ser Ser Gly Ala Pro Ile Pro Ser Ala Thr Leu Ala Pro Asn Gln
420 425 430
Asp Gln Ala Val Val Lys Pro Gln Val Thr Ile Pro Ala Phe Asn Ala
435 440 445
Gln Pro Gly Phe Arg Glu Gly Val Ser Gln Ala Thr Pro Leu Leu Lys
450 455 460
Pro Leu Asn Lys Thr His Ile Leu Leu Met Cys Ala Ala Ile Ala Leu
465 470 475 480
Ile Met Ser Gly
<210> 518
<211> 474
<212> PRT
<213> 产黄青霉
<400> 518
Met Lys His Ala Trp Ile Leu Leu Val Gly Leu Val Pro Ala Trp Gly
1 5 10 15
Leu Thr Leu His Arg Arg Asp Asn Pro Ala Val Val Gln Met Asp Ile
20 25 30
Gln Arg Lys Asp Val Leu Asp Pro Ile Thr Arg Asp Arg Arg Arg Lys
35 40 45
Arg Ser Thr Val Arg Gln Pro Leu Asp Asn Glu Glu Thr Leu Tyr Phe
50 55 60
Cys Asn Val Thr Leu Gly Thr Pro Glu Gln Lys Val Arg Leu Val Leu
65 70 75 80
Asp Thr Gly Ser Ser Asp Leu Trp Cys Asn Thr Pro Asn Ser Thr Leu
85 90 95
Cys Glu Ser Ser Glu Asn Ala Cys Ala Ser Gly Thr Tyr Asp Ser Ser
100 105 110
Ser Ser Ser Ser Tyr Ser Phe Val Ser Ser Asp Phe Asn Ile Ser Tyr
115 120 125
Ala Asp Gly Ser Gly Ala Ala Gly Asp Tyr Val Thr Asp Thr Leu Thr
130 135 140
Ile Gly Gly Thr Thr Ile Lys Asp Phe Gln Phe Gly Ile Gly Phe Ser
145 150 155 160
Ser Gly Ser Pro Glu Gly Val Leu Gly Ile Gly Tyr Thr Ala Asn Glu
165 170 175
Val Gln Val Gly Arg Asn Gly Glu Ser Ala Tyr Ala Asn Leu Pro Gln
180 185 190
Ala Met Val Asn Lys Gly Val Ile Lys Ser Asn Ala Tyr Ser Leu Trp
195 200 205
Leu Asn Asp Leu Asp Ala Asn Thr Gly Ser Ile Leu Phe Gly Gly Val
210 215 220
Asn Thr Lys Lys Tyr His Gly Ala Leu Gln Thr Val Pro Ile Gln Lys
225 230 235 240
Val Gly Gly Arg Tyr Ser Glu Phe Ile Ile Ala Leu Thr Glu Val Ser
245 250 255
Leu Thr Thr Ser Ser Gly Gln Asn Thr Tyr Ser Ser Ser Ser Ile Pro
260 265 270
Ala Gly Val Leu Leu Asp Ser Gly Ser Ser Leu Thr Tyr Leu Pro Asp
275 280 285
Ala Leu Val Gln Glu Ile Tyr Asp Asp Leu Gly Val Ser Tyr Glu Ser
290 295 300
Asp Ser Gly Ile Gly Tyr Val Glu Cys Ser Met Ala Glu Gln Ser Ile
305 310 315 320
Leu Ser Tyr Thr Phe Ser Ser Pro Thr Ile Asn Val Gly Ile Asp Glu
325 330 335
Met Ile Ile Asp Val Gly Asp Leu Tyr Phe Arg Asn Gly Lys Arg Ala
340 345 350
Cys Ile Phe Gly Ile Val Pro Ala Gly Thr Ser Thr Ala Val Leu Gly
355 360 365
Asp Thr Phe Leu Arg Ser Ala Tyr Val Val Tyr Asp Leu Glu Asn Asn
370 375 380
Glu Ile Ser Leu Ala Ser Thr Asn Phe Asn Ser Thr Glu Asn Asp Ile
385 390 395 400
Leu Glu Ile Gly Thr Gly Thr Asp Ser Val Pro Gly Ala Thr Ala Val
405 410 415
Ser Asn Pro Val Thr Thr Ala Pro Val Gly Gly Thr Ala Ala Arg Ile
420 425 430
Gly Gly Pro His Gly Gly Ser Asp Leu Ser Gly Ser Ala Thr Val Asn
435 440 445
Met Ala Ile Pro Lys Ala Thr Ala Met Pro Arg His Met Ala Val Gly
450 455 460
Leu Ala Gly Val Gly Ala Leu Leu Ala Leu
465 470
<210> 519
<211> 487
<212> PRT
<213> 米曲霉
<400> 519
Met Gln Lys Ser Trp Leu Val Leu Leu Val Ala Cys Leu Gly Leu Gln
1 5 10 15
Gly Thr Thr Ala Leu Thr Leu His Arg Arg Asp Leu Pro Ala Val Val
20 25 30
Ser Leu Asp Ile Lys Arg Asn Asn Ala Val Asp Pro Val Ala Arg Asp
35 40 45
Arg Met Arg Arg Lys Arg Asp Lys Thr Val Glu Gln Asn Leu Asp Asn
50 55 60
Glu Glu Thr Leu Tyr Phe Cys Asn Ile Thr Leu Gly Thr Pro Lys Gln
65 70 75 80
Ser Leu Arg Leu Val Leu Asp Thr Gly Ser Ser Asp Leu Trp Cys Asn
85 90 95
Ala Ala Asn Ser Thr Leu Cys Ser Ser Arg Asp Gln Pro Cys Asn Ala
100 105 110
Ser Gly Ser Tyr Asp Pro Ser Ser Ser Ser Ser Tyr Ala Tyr Thr Ser
115 120 125
Ser Asp Phe Asn Ile Ser Tyr Ala Asp Gly Thr Gly Ala Ala Gly Asp
130 135 140
Tyr Val Thr Asp Thr Ile His Ile Gly Gly Ala Thr Val Lys Asp Phe
145 150 155 160
Gln Phe Gly Val Gly Tyr Ser Ser Ser Ser Ala Glu Gly Val Leu Gly
165 170 175
Ile Gly Tyr Thr Thr Asn Glu Val Gln Val Gly Arg Leu Gly Lys Ser
180 185 190
Ala Tyr Ala Asn Leu Pro Gln Ala Met Val Lys Asn Gly Leu Ile Gln
195 200 205
Ser Asn Ala Tyr Ser Leu Trp Leu Asn Asp Leu Gly Ala Asp Thr Gly
210 215 220
Ser Ile Leu Phe Gly Gly Val Asn Thr Glu Lys Tyr His Gly Glu Leu
225 230 235 240
Gln Thr Leu Pro Ile Gln Thr Val Asn Gly Val Tyr Ser Glu Phe Ile
245 250 255
Ile Ala Leu Thr Gly Val Ser Leu Ser Ser Ala Ser Ser His His Asn
260 265 270
Tyr Ser Ser Ser Asp Ala Leu Pro Ala Ala Val Leu Leu Asp Ser Gly
275 280 285
Ser Ser Leu Thr Tyr Leu Pro Asn Ser Ile Val Gln Asp Ile Tyr Asp
290 295 300
Asp Leu Gly Val Thr Tyr Glu Ser Ser Ser Gly Val Gly Tyr Val Pro
305 310 315 320
Cys Ser Leu Ala Gln Gln Asn Ile Asn Val Thr Tyr Thr Phe Ser Ser
325 330 335
Pro Ile Ile Thr Val Gly Ile Asp Glu Leu Val Leu Asp Ala Gly Asp
340 345 350
Leu Arg Phe Arg Asn Gly Ala Arg Ala Cys Ile Phe Gly Ile Val Pro
355 360 365
Ala Gly Asp Ser Thr Ala Val Leu Gly Asp Thr Phe Leu Arg Ser Ala
370 375 380
Tyr Val Val Tyr Asp Leu Ser Asn Asn Glu Ile Ser Leu Ala Asn Thr
385 390 395 400
Lys Phe Asn Ser Thr Lys Asp Asn Ile Leu Glu Ile Gly Thr Gly Asp
405 410 415
Asp Ser Val Pro Gly Ala Thr Gln Val Ser Asn Pro Val Thr Ser Val
420 425 430
Val Ala Asp Gly Ser Gly Ala Arg Ile Gly Gly Pro Thr Gly Glu Ile
435 440 445
Phe Thr Asp Ile Pro Ser Ala Thr Ser Ser Gly Gly Ala Ala Ala Pro
450 455 460
Ala Gly Pro Thr Asp Val Pro Lys His Leu Val Leu Gly Ala Ala Ala
465 470 475 480
Ile Gly Tyr Val Leu Ala Phe
485
<210> 520
<211> 454
<212> PRT
<213> 构巢曲霉
<400> 520
Met Leu Arg Val Gly Phe Asp Ala Ser Thr Asn Glu Ile Pro Asp Pro
1 5 10 15
Val Ala Arg Asp Arg Arg Arg Arg Lys Arg Asp Gln Thr Val Ala Gln
20 25 30
Leu Ile Asp Asn Glu Glu Thr Leu Tyr Phe Cys Asn Leu Thr Val Gly
35 40 45
Thr Pro Gly Gln Ser Leu Arg Leu Ile Leu Asp Thr Gly Ser Ser Asp
50 55 60
Leu Trp Cys Asn Ala Ala Asn Ser Thr Leu Cys Ser Ser Pro Lys Asp
65 70 75 80
Pro Cys Arg Ile Ser Gly Ser Phe Asp Pro Ser Ser Ser Ser Ser Tyr
85 90 95
Ser Tyr Ile Ser Ser Asp Phe Asn Ile Thr Tyr Ala Asp Gly Thr Gly
100 105 110
Ala Ala Gly Asp Tyr Ala Thr Asp Thr Val Ser Ile Gly Gly Ala Thr
115 120 125
Ile Lys Asp Phe Gln Phe Gly Ile Gly Tyr Thr Ser Ser Ser Ala Glu
130 135 140
Gly Val Leu Gly Ile Gly Tyr Pro Ser Asn Glu Val Gln Val Ala Arg
145 150 155 160
Tyr Gly Asp Asp Ala Tyr Pro Asn Leu Pro Arg Phe Leu Met Gln Asn
165 170 175
Gly Phe Val Gln Ser Ser Ala Tyr Ser Leu Trp Leu Asn Asp Leu Glu
180 185 190
Ala Asn Thr Gly Ser Ile Leu Phe Gly Gly Val Asp Thr Glu Lys Tyr
195 200 205
Arg Gly Asp Leu Gln Thr Leu Pro Ile Gln Thr Val Asn Gly Glu Tyr
210 215 220
Ser Glu Leu Ile Ile Ala Leu Thr Gly Val Ser Leu Asp Thr Glu Ala
225 230 235 240
Arg Lys His Thr Val Ser Ser Asn Ala Leu Pro Ala Ala Val Leu Leu
245 250 255
Asp Ser Gly Ser Ser Leu Ser Tyr Leu Pro Asp Ser Ile Ala Glu Lys
260 265 270
Ile Tyr Asp Asp Leu Arg Ile Ser Tyr Glu Pro Ser Thr Gly Ala Gly
275 280 285
Tyr Ala Pro Cys Ser Leu Ala Arg Gln Asn Ile Asn Val Thr Phe Thr
290 295 300
Phe Ser Ser Pro Glu Ile Ala Val Gly Ile Asp Glu Leu Ile Ile Asp
305 310 315 320
Ala Gly Asp Leu Arg Phe Ser Asn Gly Glu Arg Ala Cys Ile Phe Gly
325 330 335
Leu Val Pro Ala Gly Asp Asn Thr Ala Val Leu Gly Asp Thr Phe Leu
340 345 350
Arg Ser Ala Tyr Val Val Tyr Asp Leu Thr Asn Asn Glu Ile Ser Ile
355 360 365
Ala Lys Thr Asn Phe Asn Ser Thr Lys Ser Asn Ile Leu Glu Ile Gly
370 375 380
Thr Gly Ser Asp Ala Val Pro Gly Ala Thr Lys Val Ser His Pro Val
385 390 395 400
Thr Ser Val Val Ala Asp Gly Ser Gly Ser Arg Ile Gly Ala Pro Thr
405 410 415
Asn Thr Glu Asp Ile Val Pro Ser Ala Ser Thr Gly Ala Ala Val Val
420 425 430
Leu Gly Arg Ser Thr Ile Ser Pro Val Leu Val Gly Ala Ala Ala Leu
435 440 445
Gly Tyr Met Phe Ala Phe
450
<210> 521
<211> 486
<212> PRT
<213> 黑曲霉
<400> 521
Met Trp Arg Val Leu Ser Leu Leu Val Ala Val Ser Phe Val Pro Leu
1 5 10 15
Glu Thr Ala Lys Ala Leu Val Leu Gln Arg Arg Glu Val Pro Ala Val
20 25 30
Val Thr Leu Asp Ile Lys Arg Asn Asp Val Ser Asp Pro Val Met Arg
35 40 45
Asp Arg Ala Arg Arg Arg Arg Asp Thr Thr Thr Val Ala Gln Ala Leu
50 55 60
Asp Asn Glu Glu Thr Leu Tyr Phe Cys Asn Ile Thr Leu Gly Thr Pro
65 70 75 80
Glu Gln Ser Leu Arg Leu Val Leu Asp Thr Gly Ser Ser Asp Leu Trp
85 90 95
Cys Asn Ala Ala Asn Ser Thr Leu Cys Ser Ser Ser Thr Asp Pro Cys
100 105 110
Ser Thr Ser Gly Thr Phe Asn Ser Asp Lys Ser Thr Thr Tyr Thr Tyr
115 120 125
Val Ser Ser Asp Phe Asn Ile Ser Tyr Ala Asp Gly Thr Gly Ala Ala
130 135 140
Gly Val Tyr Ala Thr Asp Thr Leu His Ile Gly Ala Ser Thr Leu Glu
145 150 155 160
Asp Phe Gln Phe Gly Ile Gly Tyr Thr Ser Ser Ser Ser Glu Gly Val
165 170 175
Leu Gly Ile Gly Tyr Thr Ser Asn Glu Val Gln Val Gly Arg Phe Gly
180 185 190
Asp Ser Ala Tyr Pro Asn Leu Pro Arg Ala Met Val Lys Ser Gly Leu
195 200 205
Ile Lys Ser Asn Ala Tyr Ser Leu Trp Leu Asn Asp Leu Asp Ala Asn
210 215 220
Thr Gly Ser Ile Leu Phe Gly Gly Val Asn Thr Lys Lys Tyr His Gly
225 230 235 240
Glu Leu Gln Thr Leu Pro Ile Gln Lys Val Asn Gly Ile Tyr Ser Glu
245 250 255
Phe Ile Ile Ala Leu Thr Gly Val Ala Phe Thr Ser Gly Ser Ser Ser
260 265 270
Arg Asn Tyr Ser Ser Asp Ala Leu Pro Ala Ala Val Leu Leu Asp Ser
275 280 285
Gly Ser Ser Leu Thr Tyr Leu Pro Asp Ser Ile Val Glu Asp Ile Tyr
290 295 300
Asn Asp Phe Asp Val Val Tyr Glu Gln Ser Ser Gly Val Gly Tyr Val
305 310 315 320
Pro Cys Ser Leu Ala Gln Asn Asn Thr Asn Ile Thr Tyr Thr Phe Ser
325 330 335
Ser Pro Thr Ile Thr Val Gly Val Asn Glu Leu Val Leu Asp Val Gly
340 345 350
Asp Leu Leu Phe Gln Asp Gly Glu Arg Ala Cys Val Phe Gly Ile Val
355 360 365
Pro Ala Gly Asp Ser Thr Ala Val Leu Gly Asp Thr Phe Leu Arg Ser
370 375 380
Ala Tyr Val Val Tyr Asp Leu Ala Asn Asn Glu Ile Ser Leu Ala Asn
385 390 395 400
Thr Asn Phe Asp Ala Thr Gly Asn Asp Val Leu Glu Ile Gly Thr Gly
405 410 415
Glu Asp Ser Val Pro Gly Ala Thr Lys Val Ala Asn Pro Val Thr Thr
420 425 430
Val Ala Val Asp Gly Ser Gly Ala Arg Ile Gly Gly Pro Thr Gly Ala
435 440 445
Thr Phe Thr Ser Ile Pro Ser Ala Thr Ser Gly Gly His Ala Thr Ala
450 455 460
Arg Phe Met Gly Ala Thr Arg Leu Leu Ala Phe Gly Ala Ala Val Ile
465 470 475 480
Gly Tyr Met Leu Thr Phe
485
<210> 522
<211> 761
<212> PRT
<213> 里氏木霉
<400> 522
Met Arg Ser Thr Leu Tyr Gly Leu Ala Ala Leu Pro Leu Ala Ala Gln
1 5 10 15
Ala Leu Glu Phe Ile Asp Asp Thr Val Ala Gln Gln Asn Gly Ile Met
20 25 30
Arg Tyr Thr Leu Thr Thr Thr Lys Gly Ala Thr Ser Lys His Leu His
35 40 45
Arg Arg Gln Asp Ser Ala Asp Leu Met Ser Gln Gln Thr Gly Tyr Phe
50 55 60
Tyr Ser Ile Gln Leu Glu Ile Gly Thr Pro Pro Gln Ala Val Ser Val
65 70 75 80
Asn Phe Asp Thr Gly Ser Ser Glu Leu Trp Val Asn Pro Val Cys Ser
85 90 95
Lys Ala Thr Asp Pro Ala Phe Cys Lys Thr Phe Gly Gln Tyr Asn His
100 105 110
Ser Thr Thr Phe Val Asp Ala Lys Ala Pro Gly Gly Ile Lys Tyr Gly
115 120 125
Thr Gly Phe Val Asp Phe Asn Tyr Gly Tyr Asp Tyr Val Gln Leu Gly
130 135 140
Ser Leu Arg Ile Asn Gln Gln Val Phe Gly Val Ala Thr Asp Ser Glu
145 150 155 160
Phe Ala Ser Val Gly Ile Leu Gly Ala Gly Pro Asp Leu Ser Gly Trp
165 170 175
Thr Ser Pro Tyr Pro Phe Val Ile Asp Asn Leu Val Lys Gln Gly Phe
180 185 190
Ile Lys Ser Arg Ala Phe Ser Leu Asp Ile Arg Gly Leu Asp Ser Asp
195 200 205
Arg Gly Ser Val Thr Tyr Gly Gly Ile Asp Ile Lys Lys Phe Ser Gly
210 215 220
Pro Leu Ala Lys Lys Pro Ile Ile Pro Ala Ala Gln Ser Pro Asp Gly
225 230 235 240
Tyr Thr Arg Tyr Trp Val His Met Asp Gly Met Ser Ile Thr Lys Glu
245 250 255
Asp Gly Ser Lys Phe Glu Ile Phe Asp Lys Pro Asn Gly Gln Pro Val
260 265 270
Leu Leu Asp Ser Gly Tyr Thr Val Ser Thr Leu Pro Gly Pro Leu Met
275 280 285
Asp Lys Ile Leu Glu Ala Phe Pro Ser Ala Arg Leu Glu Ser Thr Ser
290 295 300
Gly Asp Tyr Ile Val Asp Cys Asp Ile Ile Asp Thr Pro Gly Arg Val
305 310 315 320
Asn Phe Lys Phe Gly Asn Val Val Val Asp Val Glu Tyr Lys Asp Phe
325 330 335
Ile Trp Gln Gln Pro Asp Leu Gly Ile Cys Lys Leu Gly Val Ser Gln
340 345 350
Asp Asp Asn Phe Pro Val Leu Gly Asp Thr Phe Leu Arg Ala Ala Tyr
355 360 365
Val Val Phe Asp Trp Asp Asn Gln Glu Val His Ile Ala Ala Asn Glu
370 375 380
Asp Cys Gly Asp Glu Leu Ile Pro Ile Gly Ser Gly Pro Asp Ala Ile
385 390 395 400
Pro Ala Ser Ala Ile Gly Lys Cys Ser Pro Ser Val Lys Thr Asp Thr
405 410 415
Thr Thr Ser Val Ala Glu Thr Thr Ala Thr Ser Ala Ala Ala Ser Thr
420 425 430
Ser Glu Leu Ala Ala Thr Thr Ser Glu Ala Ala Thr Thr Ser Ser Glu
435 440 445
Ala Ala Thr Thr Ser Ala Ala Ala Glu Thr Thr Ser Val Pro Leu Asn
450 455 460
Thr Ala Pro Ala Thr Thr Gly Leu Leu Pro Thr Thr Ser His Arg Phe
465 470 475 480
Ser Asn Gly Thr Ala Pro Tyr Pro Ile Pro Ser Leu Ser Ser Val Ala
485 490 495
Ala Ala Ala Gly Ser Ser Thr Val Pro Ser Glu Ser Ser Thr Gly Ala
500 505 510
Ala Ala Ala Gly Thr Thr Ser Ala Ala Thr Gly Ser Gly Ser Gly Ser
515 520 525
Gly Ser Gly Asp Ala Thr Thr Ala Ser Ala Thr Tyr Thr Ser Thr Phe
530 535 540
Thr Thr Thr Asn Val Tyr Thr Val Thr Ser Cys Pro Pro Ser Val Thr
545 550 555 560
Asn Cys Pro Val Gly His Val Thr Thr Glu Val Val Val Ala Tyr Thr
565 570 575
Thr Trp Cys Pro Val Glu Asn Gly Pro His Pro Thr Ala Pro Pro Lys
580 585 590
Pro Ala Ala Pro Glu Ile Thr Ala Thr Phe Thr Leu Pro Asn Thr Tyr
595 600 605
Thr Cys Ser Gln Gly Lys Asn Thr Cys Ser Asn Pro Lys Thr Ala Pro
610 615 620
Asn Val Ile Val Val Thr Pro Ile Val Thr Gln Thr Ala Pro Val Val
625 630 635 640
Ile Pro Gly Ile Ala Ala Pro Thr Pro Thr Pro Ser Val Ala Ala Ser
645 650 655
Ser Pro Ala Ser Pro Ser Val Val Pro Ser Pro Thr Ala Pro Val Ala
660 665 670
Thr Ser Pro Ala Gln Ser Ala Tyr Tyr Pro Pro Pro Pro Pro Pro Glu
675 680 685
His Ala Val Ser Thr Pro Val Ala Asn Pro Pro Ala Val Thr Pro Ala
690 695 700
Pro Ala Pro Phe Pro Ser Gly Gly Leu Thr Thr Val Ile Ala Pro Gly
705 710 715 720
Ser Thr Gly Val Pro Ser Gln Pro Ala Gln Ser Gly Leu Pro Pro Val
725 730 735
Pro Ala Gly Ala Ala Gly Phe Arg Ala Pro Ala Ala Val Ala Leu Leu
740 745 750
Ala Gly Ala Val Ala Ala Ala Leu Leu
755 760
<210> 523
<211> 546
<212> PRT
<213> 绿木霉
<400> 523
Met Arg Ser Thr Leu Tyr Ala Leu Ala Ala Trp Pro Leu Ala Ala Gln
1 5 10 15
Ala Leu Asp Phe Ile Asp Ser Tyr Thr Ser Glu Gln Asn Gly Ile Met
20 25 30
Arg Tyr Thr Leu Thr Thr Thr Lys Gly Ala Arg Ser Lys His Leu His
35 40 45
Arg Arg Gln Asp Ser Ala Asp Leu Gln Ser Gln Gln Thr Gly Tyr Phe
50 55 60
Tyr Ser Ile Gln Leu Glu Ile Gly Thr Pro Pro Gln Ala Val Ser Val
65 70 75 80
Asn Phe Asp Thr Gly Ser Ser Glu Leu Trp Val Asn Pro Val Cys Ser
85 90 95
Lys Ala Thr Asp Pro Ala Phe Cys Glu Thr Phe Gly Arg Tyr Ser Asn
100 105 110
Ser Thr Thr Phe Val Asp Thr Gln Ala Pro Gly Gly Ile Lys Tyr Gly
115 120 125
Thr Gly Phe Val Asp Phe Thr Tyr Gly Tyr Asp Tyr Val Gln Leu Gly
130 135 140
Ser Leu Arg Ile Asn Gln Gln Val Phe Gly Val Ala Thr Asp Ser Glu
145 150 155 160
Phe Ala Ser Val Gly Ile Leu Gly Ala Gly Pro Asp Leu Ser Gly Trp
165 170 175
Lys Ser Pro Tyr Pro Phe Val Ile Asp Asn Leu Ala Lys Gln Gly Phe
180 185 190
Ile Gln Ser Arg Ala Phe Ser Leu Asp Ile Arg Gly Ile Asp Ser Asp
195 200 205
Arg Gly Ser Val Thr Tyr Gly Gly Ile Asp Val Lys Lys Phe Ser Gly
210 215 220
Pro Leu Ala Lys Arg Pro Ile Ile Pro Ala Ala Ser Ser Pro Asp Gly
225 230 235 240
Tyr Thr Arg Tyr Trp Val Tyr Met Asp Gly Met Ser Ile Thr Lys Glu
245 250 255
Asp Gly Ser Asp Leu Glu Ile Phe Asp Lys Pro Asn Gly Gln Pro Val
260 265 270
Leu Leu Asp Ser Gly Tyr Thr Val Ser Thr Leu Pro Gly Pro Leu Phe
275 280 285
Glu Lys Ile Leu Leu Ala Phe Pro Ser Ala Arg Leu Glu Ser Ser Ser
290 295 300
Gly Asp Tyr Ile Val Asp Cys Asp Ile Ile Asp Ser Pro Gly Arg Val
305 310 315 320
Asn Phe Lys Phe Gly Asp Val Val Val Asp Val Glu Tyr Lys Asp Phe
325 330 335
Ile Trp Gln Gln Pro Asp Leu Gly Ile Cys Lys Leu Gly Val Ser Gln
340 345 350
Asp Asp Gln Phe Pro Val Leu Gly Asp Thr Phe Leu Arg Ala Ala Tyr
355 360 365
Val Val Phe Asp Trp Asp Asn Gln Asn Ile His Ile Ala Ala Asn Glu
370 375 380
Asp Cys Gly Thr Glu Leu Ile Pro Ile Gly Ser Gly Pro Asp Ala Ile
385 390 395 400
Pro Ala Ser Ala Ile Gly Gln Cys Ser Ser Ser Val Asn Lys Thr Thr
405 410 415
Thr Ser Ala Val Gln Thr Thr Thr Ser Ala Ala Ala Ser Thr Ser Lys
420 425 430
Leu Ala Thr Thr Ser Ala Val Ala Thr Thr Ser Ala Ala Ala Thr Thr
435 440 445
Ser Gly Ala Ala Ser Ser Gly His Leu Pro Thr Thr Ser His His Phe
450 455 460
Gly Asn Gly Thr Val Val Tyr Ser Leu Pro Thr Leu Ser Ser Thr Ser
465 470 475 480
Val Ser Glu Gln Ser Thr Asp Ala Ala Lys Gly Thr Thr Thr Ala Ala
485 490 495
Gly Ala Gly Thr Thr Ala Ala Pro Thr Tyr Thr Ser Thr Phe Thr Thr
500 505 510
Thr Asn Val Tyr Thr Val Thr Ser Cys Pro Pro Ser Val Thr Asn Cys
515 520 525
Pro Val Gly His Val Thr Thr Glu Ile Val Thr Ser Tyr Thr Thr Trp
530 535 540
Cys Pro
545
<210> 524
<211> 549
<212> PRT
<213> 深绿木霉
<400> 524
Met Arg Ser Thr Leu Tyr Ala Leu Ala Ala Trp Pro Leu Ala Ala Gln
1 5 10 15
Ala Leu Glu Phe Leu Asp Asp Asn Lys Asn Val Glu Glu Gly Gly Ile
20 25 30
Met Arg Tyr Ser Leu Thr Ala Ala Lys Gly Ala Arg Ser Lys His Leu
35 40 45
His Arg Arg Gln Asp Thr Ala Glu Leu Gln Ser Gln Glu Thr Gly Tyr
50 55 60
Phe Tyr Ser Ile Glu Leu Glu Ile Gly Thr Pro Pro Gln Ala Val Ser
65 70 75 80
Val Asn Phe Asp Thr Gly Ser Ser Glu Leu Trp Val Asn Pro Glu Cys
85 90 95
Ser Lys Ala Thr Asp Pro Ala Phe Cys Gln Ser Phe Gly Arg Tyr Asn
100 105 110
Ala Ser Lys Thr Phe Val Asp Thr Lys Ala Asp Gly Gly Ile Lys Tyr
115 120 125
Gly Thr Gly Tyr Val Asp Phe Val Tyr Gly Tyr Asp Tyr Val Gln Leu
130 135 140
Gly Ser Ser Arg Ile Ser Gln Gln Leu Phe Gly Val Ala Thr Asp Ser
145 150 155 160
Glu Phe Ala Ser Ile Gly Ile Leu Gly Ala Gly Pro Asn Leu Ser Gly
165 170 175
Trp Thr Ser Ser Tyr Pro Leu Val Ile Asp Asn Leu Ala Lys Gln Gly
180 185 190
Phe Ile Asn Ser Arg Ala Phe Ser Leu Asp Ile Arg Gly Leu Asp Ser
195 200 205
Asp Arg Gly Ser Val Ile Tyr Gly Gly Ile Asp Thr Lys Lys Phe Ser
210 215 220
Gly Pro Leu Val Lys Arg Pro Ile Ile Pro Ala Ala Ser Ser Pro Asp
225 230 235 240
Gly Tyr Thr Arg Tyr Trp Ile Tyr Met Asp Gly Met Thr Ile Thr Thr
245 250 255
Glu Asp Gly Ser His Val Glu Ile Phe Asn Lys Pro Asn Gly Gln Pro
260 265 270
Val Leu Leu Asp Ser Gly Tyr Thr Val Ser Thr Leu Pro Ser Ser Ile
275 280 285
Phe Thr Lys Ile Leu Ala Ala Phe Pro Met Ala His Ile Glu Ala Ser
290 295 300
Ser Gly Asp Tyr Leu Val Pro Cys Asp Ile Ile Asp Thr Pro Gly Ser
305 310 315 320
Val Asn Phe Gln Phe Gly Asp Ala Thr Val Ser Val Asp Tyr Lys Asp
325 330 335
Phe Ile Trp Gln Gln Pro Asp Leu Gly Ile Cys Lys Leu Gly Val Ser
340 345 350
Gln Asp Asp Asp Phe Pro Val Leu Gly Asp Thr Phe Leu Arg Ala Ala
355 360 365
Tyr Val Val Phe Asp Trp Asp Asn Gln Asn Ile His Val Ala Lys Asn
370 375 380
Glu Asp Cys Gly Thr His Leu Ile Pro Ile Gly Lys Gly Ala Asp Ala
385 390 395 400
Val Pro Ser Gly Leu Lys Gly Asp Cys Ala Ala Ala Gly Gln Thr Thr
405 410 415
Thr Ser Ala Val Gln Thr Thr Ser Ala Ala Ala Ser Thr Ser Ala Arg
420 425 430
Val Ser Thr Ser Ala Ala Ala Thr Thr Thr Ser Val Ala Ile Thr Thr
435 440 445
Thr Ala Pro Ala Thr Ser Gly Leu Lys Ala Thr Thr Ser Ser His Arg
450 455 460
Phe Ala Asn Gly Thr Val Thr Tyr Ser Val Pro His Gly Arg Thr Thr
465 470 475 480
Val Ile Ser Glu Ile Ser Ser Ala Ala Ala Thr Gln Leu Asp Thr Thr
485 490 495
Thr Ala Pro Ala Ser Gly Thr Thr Ala Ala Pro Thr Tyr Thr Ser Thr
500 505 510
Phe Thr Thr Thr Asn Val Tyr Thr Ile Thr Ser Cys Pro Pro Ser Val
515 520 525
Thr Asn Cys Pro Val Gly His Val Thr Thr Glu Val Val Thr Ala Tyr
530 535 540
Thr Thr Trp Cys Pro
545
<210> 525
<211> 811
<212> PRT
<213> Fusarium pseudograminearum
<400> 525
Met Leu Ser Thr Leu Phe Leu Ala Val Ala Ala Pro Ala Leu Ala Thr
1 5 10 15
Ala Phe Asn Ala Pro Asn Val Pro Val Gly Thr Asp Asp Ser Ser Pro
20 25 30
Asp Gly Leu Ile Arg Phe Pro Leu Lys Val Ser Thr Gly Ala Pro Val
35 40 45
Val Lys Gly Val Thr Lys Arg Gln Asn Glu Val Ala Leu Glu Ser Gln
50 55 60
Gln Asn Gly Phe Phe Tyr Ser Ile Asp Val Thr Leu Gly Thr Pro Gly
65 70 75 80
Gln Lys Val Thr Val Asn Leu Asp Thr Gly Ser Ala Glu Leu Trp Val
85 90 95
Asn Pro Val Cys Glu Lys Ala Gln Gln Pro Ala Phe Cys Lys Gln Phe
100 105 110
Gly His Phe Gly Glu Ser Ser Thr Phe Val Asp Leu Asn Thr Thr Gly
115 120 125
Gly Val Val Tyr Gly Thr Gly Tyr Ala Tyr Trp Asn Tyr Gly Tyr Asp
130 135 140
Asn Val Val Val Gly Ser Ala Ala Ile Arg Asn Gln Val Phe Gly Val
145 150 155 160
Ala Tyr Asp Ser Ser Phe Thr Gly Val Gly Ile Met Gly Ala Gly Pro
165 170 175
Asp Leu Asn Gly Trp Asp Ala Pro Tyr Pro Leu Val Ile Asp Ser Met
180 185 190
Val Lys Gln Gly Leu Ile Lys Ser Arg Ala Leu Ser Leu Asp Ile Arg
195 200 205
Thr Leu Asn Ser Asp Arg Gly Ala Val Ile Phe Gly Gly Ile Asp Thr
210 215 220
Ala Lys Tyr Thr Gly Arg Leu Gln Lys Leu Pro Ile Ile Pro Ala Ala
225 230 235 240
Ser Ser Pro Asp Gly Leu Thr Arg Tyr Trp Val Asn Leu Asp Gly Ile
245 250 255
Ser Leu Val Gln Glu Asp Gly Thr Val Asp Ser Ala Phe Ser Gly Ser
260 265 270
Thr Pro Gln Pro Val Leu Leu Asp Ser Gly Tyr Thr Val Ser Ala Leu
275 280 285
Pro Gly Ser Ile Phe Asn Lys Ile Val Ala Ala Phe Pro Thr Ala Lys
290 295 300
Ser Asn Gly Gly Ala Tyr Val Asp Val Asp Cys Ser Val Ala Asp Ile
305 310 315 320
Lys Gly Ser Val Asp Phe Lys Phe Gly Asp Lys Thr Ile Lys Val Pro
325 330 335
Tyr Ala Asp Phe Ile Trp His Asn Asp Glu Arg Cys Val Leu Gly Val
340 345 350
Phe Gln Asp Asp Glu Phe Pro Val Leu Gly Asp Thr Phe Leu Arg Ala
355 360 365
Ala Tyr Val Val Tyr Asp Trp Asp Asn Arg Asn Val Trp Ile Ala Asn
370 375 380
Asn Glu Asp Cys Gly Thr Asn Leu Val Ala Ile Gly Thr Gly Pro Asp
385 390 395 400
Ala Val Pro Asp Leu Val Gly Gln Cys Gly Ser Asp Ser Glu Thr Thr
405 410 415
Thr Ser Ala Ser Glu Thr Ala Ser Ala Thr Gly Thr Glu Thr Glu Thr
420 425 430
Glu Thr Ala Ser Ala Thr Glu Ser Glu Ala Val Thr Ala Thr Thr Glu
435 440 445
Asp Val Thr Ser Ala Glu Thr Asp Thr Thr Thr Gly Tyr Phe Pro Thr
450 455 460
Thr Arg Tyr Ser Asn Gly Thr Val Ser Val Thr Gly Lys Gly Thr Ser
465 470 475 480
Thr Thr Lys Lys Ala Thr Gly Gly Ser Gly Glu Ser Tyr Glu Leu Pro
485 490 495
Ser Tyr Thr Asp Ser Ser Ala Ser Ala Thr Ile Thr Ser Thr Val Val
500 505 510
Thr Ser Lys Val Tyr Thr Val Thr Ala Cys Pro Pro Ser Val Thr Asn
515 520 525
Cys Pro Val Gly His Val Thr Thr Glu Thr Ile Thr Ser Leu Thr Thr
530 535 540
Tyr Cys Pro Gly Ser Asp Gly Ser Ala Pro His Pro Thr Thr Val Glu
545 550 555 560
Gly Glu Gly Ala Pro His Pro Thr Gly Ala Ala His Leu Thr Ser Tyr
565 570 575
Val Thr Ala Thr Asn Met His Thr Val Thr Ser Cys Pro Gly Glu Gly
580 585 590
Ala Cys His Thr Ser Val Ile Thr Glu Ile Val His Asn Thr Gln Ile
595 600 605
Ala Ser Pro Glu Glu Pro Thr Gly Ile Phe Thr Ile Pro Glu Ala Ile
610 615 620
Gln Cys Gly Ala Gly Asn Phe Gly Cys Lys Glu Ala Thr Thr Lys Gly
625 630 635 640
Tyr Arg Thr Val Thr Ile Thr Pro Val Val Lys Ala Pro Lys Pro Thr
645 650 655
Pro Val Pro Gly Tyr Cys Ala Thr Cys Gly Pro Leu Lys Gly His Gly
660 665 670
His His Asn Asp Asp Asp Asp Lys Glu Thr Ala Thr Gly Gly Tyr Thr
675 680 685
Ala Pro Gly Lys Gln Thr Gly Gly Tyr Asp Gln Pro Thr Gln Ala His
690 695 700
Pro Gly Gln Asp Tyr Pro Arg Pro Thr Gly Asp Ser Pro Lys Pro Thr
705 710 715 720
Gly Gly Ser Asp Lys Pro Thr Gly Asp Ser Asp Lys Pro Ser Gly Asp
725 730 735
Ala Pro Lys Pro Asn Pro Leu Glu Glu Leu Gln Thr Tyr Arg Pro Thr
740 745 750
Asn Val Phe Pro Gln Ile Pro Ser Ser Val Ala Thr Val Val Lys Pro
755 760 765
Thr Gly Thr Pro Gln Ala Gly Gly Pro Ser Glu Pro Ala Gly Thr Pro
770 775 780
Pro Val Val Ile Asn Gly Ala Ser Gly Trp Asn Val Pro Val Val Ala
785 790 795 800
Ala Val Val Val Gly Ala Phe Val Ala Ala Leu
805 810
<210> 526
<211> 824
<212> PRT
<213> Metarhizium acridum
<400> 526
Met Met Leu Ser Ser Leu Phe Ser Leu Ala Ala Leu Pro Leu Ala Thr
1 5 10 15
Gln Ala Leu Ala Glu Phe Pro Val Asp Asn Arg Met Val Gln Glu Pro
20 25 30
Gly Leu Ile Arg Phe Pro Leu Asp Ile Ala Ala Gly Ala Pro Thr Lys
35 40 45
Asn Arg Met Pro Lys Arg Gln Asn Glu Ile Gly Val Asn Ala Glu Lys
50 55 60
Ser Gly Phe Phe Tyr Ser Ile Glu Leu Gln Val Gly Thr Pro Ala Gln
65 70 75 80
Ala Val Arg Val Asn Phe Asp Thr Gly Ser Ala Glu Leu Trp Ile Asn
85 90 95
Pro Val Cys Ser Lys Ser Thr Asp Pro Gly Phe Cys Asp Lys Leu Gly
100 105 110
Arg Phe Asn Gly Ser Gln Thr Phe Val Asp Thr Asn Thr Thr His Glu
115 120 125
Ile Arg Tyr Gly Thr Gly Phe Ala Lys Leu Glu Tyr Gly Tyr Asp Tyr
130 135 140
Val Gln Ile Gly Ser Ala Lys Ile Ser Gln Gln Met Phe Gly Val Ala
145 150 155 160
Thr Asp Ser Glu Phe Ala Ser Ala Gly Ile Leu Gly Ala Ala Pro Gln
165 170 175
Leu Lys Gly Trp Lys Ser Asp Tyr Pro Leu Val Leu Asp Asn Leu Ala
180 185 190
Ala Gln Gly Phe Ile Lys Ser Arg Ala Phe Ser Leu Asp Ile Arg Ser
195 200 205
Ile Ala Ser Lys Arg Gly Ser Val Val Phe Gly Gly Ile Asp Thr Lys
210 215 220
Lys Phe Ser Gly His Leu Glu Lys Arg Pro Ile Ile Pro Ala Thr Glu
225 230 235 240
Ser Pro Asp Lys Leu Thr Arg Tyr Trp Ile Tyr Leu Asp Gly Val Ser
245 250 255
Val Thr Lys Glu Asp Gly Ser Lys Ala Val Ile Phe Asp Gln Ala Asn
260 265 270
Gly Gln Pro Val Leu Leu Asp Ser Gly Tyr Thr Val Ser Ala Leu Pro
275 280 285
Thr Arg His Phe Asn Lys Ile Lys Asp Ala Phe Pro Gly Val Ser Ala
290 295 300
Pro Pro Glu Asp Asp Lys Ser Gly Leu Tyr Arg Val Pro Cys Asp Val
305 310 315 320
Gly Ser Lys Asn Gly Ser Val Asn Phe Lys Phe Gly Lys Thr Glu Ile
325 330 335
Val Val Pro Tyr Asp Asp Phe Ile Trp Lys Gln Pro Glu Asn Asn Leu
340 345 350
Cys Val Leu Gly Val Thr Pro Asp Asp Glu Phe Pro Val Leu Gly Asp
355 360 365
Thr Phe Leu Arg Gly Ala Tyr Ala Val Tyr Asp Gln Asp Asn Arg Asn
370 375 380
Ile His Leu Ala Asn Asn Glu Asp Cys Gly Ser Ser Leu Val Ala Ile
385 390 395 400
Gly Thr Gly Pro Asp Ala Val Pro Ser Val Glu Gly Asp Cys Gly Arg
405 410 415
Pro Glu Pro Thr Thr Thr Ser Thr His Ser Lys Thr Thr Leu Ala Thr
420 425 430
Ile Pro Thr Gly Asn Ser Thr Ile Ser Ser Ala Lys His Thr Thr Ser
435 440 445
Ser Ser Ser Val Val Ser Pro Thr Ser Thr His Ser Thr Thr Ser Ser
450 455 460
Ala Ala Ile Pro Thr Ala His Asn Ser Ser Val Ile Pro Ser Ala Lys
465 470 475 480
Ser Thr Ala Ser Ser Ser Ser Thr Ala Ser Leu Thr Ile Pro Ala Thr
485 490 495
Arg Asn Ser Thr Val Ile Ser Ala Lys Pro Thr Thr Ala Ser Thr Leu
500 505 510
Val Ser Thr Gly His Glu Asp Asn Thr Val Leu Thr Thr Ser Lys Leu
515 520 525
Ile Ser Thr Thr Ser Lys Pro Ile Ser Ile Thr Ser Lys Ser Thr Ser
530 535 540
Thr Thr Ser Asn Pro Leu Pro Thr Gly Thr Glu Gln Asn Thr Thr Val
545 550 555 560
Pro Val Thr His Thr Ser Thr Phe Thr Thr Thr Ser Val His Thr Ile
565 570 575
Leu Ser Cys Pro Pro Tyr Val Thr Gly Cys Pro Ile Gly Ser Val Thr
580 585 590
Thr Glu Thr Ile Thr Gly Val Thr Thr Trp Cys Pro Gly Asn Thr Ala
595 600 605
Pro Ser Ala Lys Pro Pro Gln Thr Thr Glu Thr Lys Thr Pro Pro Pro
610 615 620
Ala Thr Val Ile Thr Val Gln Pro Thr Val Thr Ser Ala Pro Ser Ile
625 630 635 640
Thr Ser Val Phe Thr Thr Thr Lys Thr His Ala Ile Thr Ser Cys Ser
645 650 655
Glu Gln Ala Ser Cys Asn Lys Gly His Val Thr Thr Glu Val Val Thr
660 665 670
Ser Thr Thr Tyr Leu Cys Pro Glu Ser Thr Ala Thr Phe Thr Ile Pro
675 680 685
Arg Thr His Thr Cys Arg Glu Gly Asp Asn Gly Cys Arg Ser Gly Asp
690 695 700
Val Val Thr Thr Val Phe Thr Val Thr Val Glu Pro Gln Pro Thr Ala
705 710 715 720
Asp Lys Pro Thr Pro Val Pro Gly Cys Gly Asp Asn Cys Val Leu Pro
725 730 735
Pro Pro Ala Gln Thr Arg Pro Ala Ala Glu Thr Thr Ala Ala Glu Thr
740 745 750
Gln Pro Ser Ile Thr Ala Ile Ile Thr Ala Pro Gly Pro Val Pro Thr
755 760 765
Val Pro Gly Thr Asn Gly Thr Ile Thr Thr Thr Ser Lys Pro Gly Asp
770 775 780
Thr Pro Cys Ser Thr Cys Ser Pro Glu Thr Thr Ser Thr Pro Met Thr
785 790 795 800
Ala Gly Ala Ser Gly Phe Arg Ile Pro Ile Met Ala Val Ala Met Ile
805 810 815
Val Gly Ala Leu Thr Val Ile Leu
820
<210> 527
<211> 811
<212> PRT
<213> 玉米赤霉
<400> 527
Met Leu Ser Thr Leu Phe Leu Ala Val Ala Ala Pro Ala Leu Ala Thr
1 5 10 15
Ala Phe Asn Ala Pro Asn Val Pro Val Gly Thr Asp Asp Ser Ser Pro
20 25 30
Asp Gly Leu Ile Arg Phe Pro Leu Lys Val Ser Thr Gly Ala Pro Val
35 40 45
Val Lys Gly Val Thr Lys Arg Gln Asn Glu Val Ala Leu Glu Ser Gln
50 55 60
Gln Asn Gly Phe Phe Tyr Ser Ile Asp Val Thr Leu Gly Thr Pro Gly
65 70 75 80
Gln Lys Val Thr Val Asn Leu Asp Thr Gly Ser Ala Glu Leu Trp Val
85 90 95
Asn Pro Val Cys Glu Lys Ala Gln Gln Pro Ala Phe Cys Lys Gln Phe
100 105 110
Gly His Phe Gly Glu Ser Ser Thr Phe Val Asp Leu Asn Thr Thr Gly
115 120 125
Gly Val Val Tyr Gly Thr Gly Tyr Ala Tyr Trp Asn Tyr Gly Tyr Asp
130 135 140
Asn Val Val Val Gly Ser Ala Ala Ile Arg Asn Gln Val Phe Gly Val
145 150 155 160
Ala Tyr Asp Ser Ser Phe Thr Gly Val Gly Ile Met Gly Ala Gly Pro
165 170 175
Asp Leu Asn Gly Trp Asp Ala Pro Tyr Pro Leu Val Ile Asp Ser Met
180 185 190
Val Lys Gln Gly Leu Ile Lys Ser Arg Ala Leu Ser Leu Asp Ile Arg
195 200 205
Thr Ile Asn Ser Asp Arg Gly Ala Val Ile Phe Gly Gly Ile Asp Thr
210 215 220
Ala Lys Tyr Thr Gly Arg Leu Gln Lys Leu Pro Ile Ile Pro Ala Ala
225 230 235 240
Ser Ser Pro Asp Gly Leu Thr Arg Tyr Trp Val Ser Leu Asp Gly Ile
245 250 255
Ser Leu Val Gln Glu Asp Gly Thr Val Asp Ser Ala Phe Ser Gly Ser
260 265 270
Thr Pro Gln Pro Val Leu Leu Asp Ser Gly Tyr Thr Val Ser Ala Leu
275 280 285
Pro Gly Ser Ile Phe Asn Lys Ile Val Ala Ala Phe Pro Thr Ala Lys
290 295 300
Ser Asn Gly Gly Ala Tyr Val Asp Val Asp Cys Ser Val Ala Asp Ile
305 310 315 320
Lys Gly Ser Val Asp Phe Lys Phe Gly Asp Lys Thr Ile Lys Val Pro
325 330 335
Tyr Ala Asp Phe Ile Trp His Asn Asp Asp Arg Cys Val Leu Gly Val
340 345 350
Phe Gln Asp Asp Glu Phe Pro Val Leu Gly Asp Thr Phe Leu Arg Ala
355 360 365
Ala Tyr Val Val Tyr Asp Trp Asp Asn Arg Asn Val Trp Ile Ala Asn
370 375 380
Asn Glu Asp Cys Gly Thr Asn Leu Val Ala Ile Gly Thr Gly Pro Asp
385 390 395 400
Ala Val Pro Asp Leu Val Gly Gln Cys Gly Ser Asp Ser Glu Thr Thr
405 410 415
Thr Ser Ala Ser Glu Thr Ala Ser Ala Thr Glu Thr Glu Thr Glu Thr
420 425 430
Glu Thr Ala Ser Ala Thr Glu Ser Glu Ala Val Thr Ala Thr Thr Glu
435 440 445
Asp Val Thr Ser Ala Glu Thr Asp Thr Thr Thr Gly Tyr Phe Pro Thr
450 455 460
Thr Arg Tyr Ser Asn Ser Thr Ala Ser Val Thr Gly Lys Gly Thr Ser
465 470 475 480
Thr Thr Lys Lys Ala Thr Gly Gly Ser Gly Glu Ser Tyr Glu Leu Pro
485 490 495
Ser Tyr Thr Asp Ser Ser Ala Ser Thr Thr Ile Thr Ser Thr Val Val
500 505 510
Thr Ser Lys Val Tyr Thr Val Thr Ala Cys Pro Pro Ser Val Thr Asn
515 520 525
Cys Pro Val Gly Gln Val Thr Thr Glu Thr Ile Thr Ser Leu Thr Thr
530 535 540
Tyr Cys Pro Gly Ser Asp Asp Ser Ser Ser His Ser Thr Thr Val Glu
545 550 555 560
Gly Glu Gly Ala Thr His Pro Thr Gly Ala Val His Leu Thr Ser Tyr
565 570 575
Val Thr Ala Thr Asn Met His Thr Ile Thr Ser Cys Pro Gly Glu Gly
580 585 590
Ala Cys His Thr Ser Val Ile Thr Glu Ile Val His Asn Thr Gln Ile
595 600 605
Ala Ser Pro Glu Glu Pro Thr Gly Ile Phe Thr Ile Pro Glu Ala Ile
610 615 620
Gln Cys Gly Ala Gly Asn Phe Gly Cys Lys Glu Ala Thr Thr Lys Gly
625 630 635 640
Tyr Arg Thr Val Thr Ile Thr Pro Val Val Lys Ala Pro Lys Pro Thr
645 650 655
Pro Val Pro Gly Tyr Cys Ala Thr Cys Gly Pro Leu Lys Gly His Gly
660 665 670
His His Asn Asp Asp Asp Lys Glu Thr Ala Thr Gly Gly Tyr Thr Ala
675 680 685
Pro Gly Lys Gln Thr Gly Gly Tyr Asp Gln Pro Thr Gln Ala His Pro
690 695 700
Gly Gln Asp Tyr Pro Arg Pro Thr Gly Gly Asp Ser Pro Lys Pro Thr
705 710 715 720
Gly Asp Ser His Asn Pro Thr Gly Asp Ser Asp Lys Pro Ser Gly Asp
725 730 735
Ala Pro Lys Pro Asn Pro Leu Glu Glu Leu His Thr Tyr Arg Pro Thr
740 745 750
Asn Val Phe Pro Gln Ile Pro Ser Ser Val Ala Thr Val Val Lys Pro
755 760 765
Thr Gly Thr Pro Gln Ala Gly Gly Pro Ser Glu Pro Ala Gly Thr Pro
770 775 780
Pro Val Val Ile Asn Gly Ala Ser Gly Trp Asn Val Pro Val Val Ala
785 790 795 800
Ala Val Val Val Gly Ala Phe Val Ala Ala Leu
805 810
<210> 528
<211> 668
<212> PRT
<213> 嗜热毁丝霉
<400> 528
Met Ser Phe Ala Leu Tyr Ala Ala Ala Leu Leu Pro Val Ala Val Leu
1 5 10 15
Gly Ala Gly Leu Ser Val Pro Glu Asp Asn Arg Met Val Gln Gln Asp
20 25 30
Gly Leu Leu Arg Tyr Pro Leu Met Pro Arg Leu Gly Asn Leu Leu Phe
35 40 45
Gly Lys His Ala Asn Ile Thr Arg Arg Gln Ile Asp Thr Gly Ile Phe
50 55 60
Asp Pro Leu Ser Gly Thr Leu Tyr Thr Ile Glu Leu Thr Leu Gly Thr
65 70 75 80
Pro Gly Gln Thr Val Pro Val Gln Phe Asp Thr Gly Ser Asp Met Leu
85 90 95
Trp Val Asn Pro Val Cys Ser Lys Ala Ala Glu Pro Glu Phe Cys Ala
100 105 110
Ala Gln Pro Arg Phe Thr Asp Ser Ser Thr Leu Val Asp Phe Gly Glu
115 120 125
Gln Gly Asn Ile Thr Tyr Gly Thr Gly Tyr Ala Tyr Tyr Glu Tyr Val
130 135 140
Ala Asp Tyr Val Ala Ile Gly Ser Ala Arg Ile Thr Gln Gln Val Phe
145 150 155 160
Gly Val Ala Leu Asp Ser Ala His Ala Asp Val Gly Ile Phe Gly Ala
165 170 175
Gly Pro Asn Leu Asp Gly Trp Asp Ser Ala Tyr Pro Leu Val Val Asp
180 185 190
Ser Leu Ala Gln Gln Gly Tyr Thr Ser Ser Arg Ala Phe Ser Met Asp
195 200 205
Leu Lys Gly Phe Glu Ser Ala Arg Gly Ser Val Ile Phe Gly Gly Ile
210 215 220
Asp Thr Lys Lys Tyr Arg Gly Ser Leu Ile Lys Arg Leu Ile Ile Pro
225 230 235 240
Ala Ala Glu Ser Pro Asp Gly Tyr Thr Arg Phe Trp Ile Tyr Leu Asp
245 250 255
Gly Ile Ser Val Asn Gln Pro Asp Gly Asp Val Val Thr Val Phe Ser
260 265 270
Thr Pro Asp Gly Gly Lys Gly Gln Pro Val Leu Leu Asp Ser Gly Tyr
275 280 285
Thr Leu Ser Ala Leu Pro Arg Pro Ile Phe Gln Lys Leu Val Ala Ala
290 295 300
Phe Pro Ser Ala Gln Tyr Val Ser Ser Ala Asp Val Tyr Val Val Asp
305 310 315 320
Cys Val Asp His Gly Glu Gly Gly Ser Leu Asp Phe Ile Phe Gly Gly
325 330 335
Lys Thr Ile Asn Val Pro Tyr His Glu Phe Val Trp Ala Gln Pro Glu
340 345 350
Ser Asn Thr Cys Val Leu Gly Ala Phe Glu Asp Asp Phe Pro Val Leu
355 360 365
Gly Asp Thr Phe Leu Arg Ser Ala Tyr Val Val Tyr Asp Trp Asp Asn
370 375 380
Arg Asn Ile Tyr Leu Ala Gln Ser Asp Asp Cys Gly Ser Asn Leu Val
385 390 395 400
Ala Ile Gly Ser Gly Pro Asp Ala Val Pro Ser Ile Val Gly Glu Cys
405 410 415
Gly Lys Pro Lys Pro Thr Ser Thr Ser Thr Phe Ser Lys Thr Ser Ser
420 425 430
Lys Thr Ser Thr Ala Ser Lys Thr Ser Ser Thr Ser Asp Ser Thr Ser
435 440 445
Ser Ser Ser Ser His Val Thr Thr Ser Ser Ser Ser Thr Thr Ala Thr
450 455 460
Thr Leu Ser Thr His Lys Pro Pro Phe Pro Thr Ala Ser Gly Asn Phe
465 470 475 480
Thr Thr Thr Arg Ser Pro Thr Thr Thr Thr Ala Ser Ser Thr Ile Ser
485 490 495
Lys Ser Thr Leu Thr Ile Thr Ser Ala Thr Thr Tyr Thr Ile Thr Ser
500 505 510
Cys Pro Pro Thr Val Thr Arg Cys Pro Ala His Glu Val Thr Thr Glu
515 520 525
Ile Ile Thr Lys Thr Thr Ala Val Cys Pro Glu Thr Thr Ala Thr Tyr
530 535 540
Thr Ile Pro Arg Thr Ile Thr Cys Pro Gly Ser Gly Gly Gly Asp Asp
545 550 555 560
Cys Pro Pro Gly Ala Thr Arg Thr Thr Thr Leu Thr Val Thr Leu Ser
565 570 575
Pro Val Gly Pro Thr Asp Arg Thr Thr His Val Val Pro Gly Val Thr
580 585 590
Thr Thr Thr Pro Thr Thr Ile Thr Ala Pro Pro Thr Gly Gln Thr Thr
595 600 605
Thr Thr Leu Val Pro Ala Leu Pro Pro Thr Thr Thr Thr Met Ser Gly
610 615 620
His Arg Gly Ile Asn Gly Thr Val Thr Ala Thr Ser Lys Pro Pro Ala
625 630 635 640
Val Thr Ala Gly Ser Ala Lys Val Gly Leu Val Ser Gly Ala Thr Ala
645 650 655
Ile Val Ala Gly Val Met Ala Val Leu Met Ala Leu
660 665
<210> 529
<211> 551
<212> PRT
<213> 粗糙链孢霉
<400> 529
Met Leu Pro Val Pro Leu Thr Thr Leu Ser Leu Tyr Val Val Ala Leu
1 5 10 15
Leu Ser Pro Pro Ala Ala Ala Gly Val Leu Ala Ser Ala Thr Thr Lys
20 25 30
Leu Pro Ile Lys Leu Pro Ile Ser Pro Ala Gln Gly His Arg Ser Ser
35 40 45
Thr Ala Ala Ser Pro Ser Leu Thr Ser Arg Ser Ser Ser Ser Gly Asn
50 55 60
Gly Phe Ile Arg Ala Ser Val His Ala Ala His Gly Ala Pro Pro Lys
65 70 75 80
Leu Arg Arg Arg Gln Glu Asp Glu Gly Leu Lys Asn Gln Asn Leu Gly
85 90 95
Thr Thr Tyr Thr Ile Asp Ile Asp Ile Gly Thr Pro Pro Gln Thr Val
100 105 110
Thr Leu Ile Leu Asp Thr Gly Ser Pro Asp Leu Trp Val Asn Pro Gln
115 120 125
Cys Glu Thr Ser Gly Gln Glu Lys Tyr Cys Asn Ser Phe Arg Gln Phe
130 135 140
Asp Tyr Thr Lys Ser Lys Thr Ile Gln Asp Thr Gly Ala Ala Asp Ile
145 150 155 160
Leu Lys Tyr Gly Lys Gly Asn Val Thr Ile Glu Tyr Val Thr Asp Asp
165 170 175
Val Ile Ile Gly Ser Ala Lys Ile Lys Ser Gln Ile Leu Gly Ile Gly
180 185 190
Phe Glu Ser Ile Asp Ile Pro Leu Gly Ile Leu Gly Leu Ser Pro Ser
195 200 205
Val Ser Pro Asp Gly Thr Ser Pro Tyr Pro Tyr Leu Leu Asp Ser Met
210 215 220
Ala Ser Gln Gly Ile Ile Ser Ser Arg Ala Phe Ser Leu Asp Leu Arg
225 230 235 240
Ser Ile Asp Asn Pro Ser Gly Ala Ile Ile Phe Gly Gly Val Asp Leu
245 250 255
Gly Lys Phe Ser Gly Ser Leu Ala Lys Leu Pro Met Leu Asp Pro Ser
260 265 270
Gln Thr Pro Ala Gly Val Asp Arg Tyr Trp Ile Val Leu Ser Gly Val
275 280 285
Gly Met Thr Tyr Pro Asp Gly Glu Glu Val Glu Ser Glu Glu Ile Gly
290 295 300
Val Pro Val Phe Leu Asp Ser Gly Gly Thr Leu Ser Arg Leu Pro Glu
305 310 315 320
Thr Ile Phe Gln Ala Ile Gly Asp Ser Phe Pro Gly Ser Gln Tyr Asp
325 330 335
Pro Glu Ser Gly Phe Tyr Ile Val Asp Cys Ala Val Ala Glu Gln Ala
340 345 350
Gly Ser Val Asp Phe Ile Phe Gly Ser Ser Gly Ser Arg Ser Ser Lys
355 360 365
Lys Ile Arg Val Pro Tyr Gly Asp Phe Val Trp Glu Val Gln Thr Gly
370 375 380
Val Cys Val Val Gly Val Leu Pro Thr Asp Asp Glu Pro Val Phe Gly
385 390 395 400
Asp Ser Phe Leu Arg Ala Ala Tyr Val Val Phe Asp Gln Asp Asn Arg
405 410 415
Asn Leu His Leu Ala Gln Ala Ala Asn Cys Gly Glu Gln Ile Val Glu
420 425 430
Ile Gly Ser Gly Gln Asp Ala Val Pro Ser Ser Thr Gly Lys Cys Lys
435 440 445
Asp Gly Ser Ala Gly Ser Thr Lys Thr Ala Gly Gly Gly Gly Leu Asp
450 455 460
Val Thr Ala Thr Arg Ala Pro Thr Arg Thr Ala Gly Gly Ser Gly Pro
465 470 475 480
Ala Val Thr Asn Ser Asp Phe Gly Pro Gly Pro Ala Gly Thr Arg Val
485 490 495
Ser Thr Gly Gly Ile Gly Leu Pro Thr Gly Thr Gly Gly Gly Gly Gly
500 505 510
Ser Gly Asp Gly Asn Gly Asn Asn Asp Asp Asp Asp Ser Ala Ala Ser
515 520 525
Gly Leu Asp Val Gly Val Thr Ala Ala Ala Val Leu Ala Gly Leu Asn
530 535 540
Met Leu Ile Val Trp Leu Leu
545 550
<210> 530
<211> 477
<212> PRT
<213> 里氏木霉
<400> 530
Met Arg Phe Val Gln Tyr Val Ser Leu Ala Gly Leu Phe Ala Ala Ala
1 5 10 15
Thr Val Ser Ala Gly Val Val Thr Val Pro Phe Glu Lys Arg Asn Leu
20 25 30
Asn Pro Asp Phe Ala Pro Ser Leu Leu Arg Arg Asp Gly Ser Val Ser
35 40 45
Leu Asp Ala Ile Asn Asn Leu Thr Gly Gly Gly Tyr Tyr Ala Gln Phe
50 55 60
Ser Val Gly Thr Pro Pro Gln Lys Leu Ser Phe Leu Leu Asp Thr Gly
65 70 75 80
Ser Ser Asp Thr Trp Val Asn Ser Val Thr Ala Asp Leu Cys Thr Asp
85 90 95
Glu Phe Thr Gln Gln Thr Val Gly Glu Tyr Cys Phe Arg Gln Phe Asn
100 105 110
Pro Arg Arg Ser Ser Ser Tyr Lys Ala Ser Thr Glu Val Phe Asp Ile
115 120 125
Thr Tyr Leu Asp Gly Arg Arg Ile Arg Gly Asn Tyr Phe Thr Asp Thr
130 135 140
Val Thr Ile Asn Gln Ala Asn Ile Thr Gly Gln Lys Ile Gly Leu Ala
145 150 155 160
Leu Gln Ser Val Arg Gly Thr Gly Ile Leu Gly Leu Gly Phe Arg Glu
165 170 175
Asn Glu Ala Ala Asp Thr Lys Tyr Pro Thr Val Ile Asp Asn Leu Val
180 185 190
Ser Gln Lys Val Ile Pro Val Pro Ala Phe Ser Leu Tyr Leu Asn Asp
195 200 205
Leu Gln Thr Ser Gln Gly Ile Leu Leu Phe Gly Gly Val Asp Thr Asp
210 215 220
Lys Phe His Gly Gly Leu Ala Thr Leu Pro Leu Gln Ser Leu Pro Pro
225 230 235 240
Ser Ile Ala Glu Thr Gln Asp Ile Val Met Tyr Ser Val Asn Leu Asp
245 250 255
Gly Phe Ser Ala Ser Asp Val Asp Thr Pro Asp Val Ser Ala Lys Ala
260 265 270
Val Leu Asp Ser Gly Ser Thr Ile Thr Leu Leu Pro Asp Ala Val Val
275 280 285
Gln Glu Leu Phe Asp Glu Tyr Asp Val Leu Asn Ile Gln Gly Leu Pro
290 295 300
Val Pro Phe Ile Asp Cys Ala Lys Ala Asn Ile Lys Asp Ala Thr Phe
305 310 315 320
Asn Phe Lys Phe Asp Gly Lys Thr Ile Lys Val Pro Ile Asp Glu Met
325 330 335
Val Leu Asn Asn Leu Ala Ala Ala Ser Asp Glu Ile Met Ser Asp Pro
340 345 350
Ser Leu Ser Lys Phe Phe Lys Gly Trp Ser Gly Val Cys Thr Phe Gly
355 360 365
Met Gly Ser Thr Lys Thr Phe Gly Ile Gln Ser Asp Glu Phe Val Leu
370 375 380
Leu Gly Asp Thr Phe Leu Arg Ser Ala Tyr Val Val Tyr Asp Leu Gln
385 390 395 400
Asn Lys Gln Ile Gly Ile Ala Gln Ala Thr Leu Asn Ser Thr Ser Ser
405 410 415
Thr Ile Val Glu Phe Lys Ala Gly Ser Lys Thr Ile Pro Gly Pro Ala
420 425 430
Ser Thr Gly Asp Asp Ser Asp Asp Ser Ser Asp Asp Ser Asp Glu Asp
435 440 445
Ser Ala Gly Ala Ala Leu His Pro Thr Phe Ser Ile Ala Leu Ala Gly
450 455 460
Thr Leu Phe Thr Ala Val Ser Met Met Met Ser Val Leu
465 470 475
<210> 531
<211> 470
<212> PRT
<213> 绿木霉
<400> 531
Met Arg Ser Ser Val Gln Tyr Ile Ser Leu Ala Gly Leu Ile Ala Ala
1 5 10 15
Gly Thr Val Ser Ala Gly Ile Val Ser Ile Pro Leu Glu Lys Arg Tyr
20 25 30
Ile Ile Asn Asp Pro Ala Pro Ser Leu Leu Arg Arg Asp Gly Ser Ile
35 40 45
Ser Leu Asp Ala Leu Asn Asn Ile Thr Gly Gly Gly Tyr Tyr Ala Glu
50 55 60
Phe Ser Ile Gly Thr Pro Pro Gln Lys Leu Ser Phe Leu Leu Asp Thr
65 70 75 80
Gly Ser Ser Asp Thr Trp Val Asn Ser Val Lys Ala Asp Leu Cys Thr
85 90 95
Ser Pro Ser Val Gln Gln Thr Val Gly Glu Tyr Cys Tyr Lys Gln Phe
100 105 110
Asn Pro Ser Ser Ser Ser Ser Tyr Lys Ser Ser Thr Glu Asp Phe Asp
115 120 125
Ile Lys Tyr Leu Asp Gly Arg Arg Ile Gln Gly Thr Tyr Phe Lys Asp
130 135 140
Thr Val Thr Ile Asn Gly Ala Ser Ile Lys Ser Gln Gln Leu Gly Leu
145 150 155 160
Ala Leu Val Ser Val Arg Gly Thr Gly Ile Met Gly Leu Gly Phe Thr
165 170 175
Asp Asn Val Ala Ala Asp Val Lys Tyr Pro Thr Ile Ile Asp Asn Met
180 185 190
Val Ser Gln Asn Val Ile Pro Val Pro Ala Phe Ser Leu Tyr Leu Asn
195 200 205
Asp Leu Gln Thr Ser Gln Gly Ala Ile Leu Phe Gly Gly Val Asp Thr
210 215 220
Asp Lys Phe His Asp Gly Leu Ala Thr Leu Pro Leu Gln Pro Leu Pro
225 230 235 240
Ala Asn Val Ala Thr Thr Gln Glu Ile Val Met Tyr Asn Val Gln Leu
245 250 255
Asn Gly Phe Thr Ala Ser Gly Val Asp Thr Pro Ala Val Asn Gly Ser
260 265 270
Ala Ile Leu Asp Ser Gly Ser Thr Leu Thr Leu Leu Pro Asp Ala Val
275 280 285
Ala Gln Ser Leu Trp Lys Lys Tyr Gly Val Ile Asn Leu Gln Gly Leu
290 295 300
Pro Ile Pro Phe Val Asp Cys Gly Lys Ala Asn Thr Lys Asp Ala Thr
305 310 315 320
Phe Asn Phe Lys Phe Thr Asn Lys Thr Ile Lys Val Pro Ile Asp Glu
325 330 335
Met Val Ile Asn Asn Leu Ala Ser Ile Gln Gly Glu Ile Met Ser Asp
340 345 350
Pro Thr Leu Ser Ser Leu Phe Lys Gly Trp Ser Gly Val Cys Thr Phe
355 360 365
Gly Ile Ser Ser Thr Arg Asn Tyr Gly Ile Arg Thr Asp Asn Phe Ile
370 375 380
Leu Leu Gly Asp Thr Phe Leu Arg Ser Ala Tyr Val Val Tyr Asp Leu
385 390 395 400
Gln Asn Lys Gln Ile Gly Ile Ala Gln Ala Thr Leu Asn Ser Thr Thr
405 410 415
Ser Ser Ile Val Glu Phe Gln Lys Gly Ser Lys Thr Ile Pro Gly Pro
420 425 430
Val Ser Thr Gly Ala Asp Asp Ser Gly Ser Ala Gly Ser Ala Ile Tyr
435 440 445
Pro Thr Phe Ser Ile Ala Leu Ala Gly Thr Val Phe Thr Ala Leu Ser
450 455 460
Met Met Leu Ser Ala Leu
465 470
<210> 532
<211> 480
<212> PRT
<213> 深绿木霉
<400> 532
Met Arg Ser Thr Leu Gln Tyr Ala Ser Leu Ala Ala Leu Val Ala Ala
1 5 10 15
Gly Thr Ala Ser Ala Gly Val Val Ser Val Pro Phe Glu Lys Arg Phe
20 25 30
Leu Asp Ser Asn Pro Leu Pro Ser Leu Leu Arg Arg Asp Gly Thr Val
35 40 45
Ala Leu Asp Ala Leu Asn Asn Ile Thr Gly Gly Gly Tyr Tyr Ala Glu
50 55 60
Phe Ser Val Gly Thr Pro Pro Gln Lys Leu Ser Phe Met Leu Asp Thr
65 70 75 80
Gly Ser Ser Asp Thr Trp Val Asn Ser Val Asp Ala Asp Leu Cys Thr
85 90 95
Asp Ser Ile Ile Gln Glu Glu Val Gly Glu Phe Cys Ser Lys Gln Phe
100 105 110
Asp Gln Thr Lys Ser Lys Ser Phe Asn Ser Thr Arg Gln Leu Phe Asn
115 120 125
Ile Thr Tyr Leu Asp Gly Arg Asn Ile Arg Gly Arg Tyr Phe Lys Asp
130 135 140
Thr Val Thr Ile Asn Asn Ala Ala Ile Lys Asn Gln Gln Met Gly Leu
145 150 155 160
Ala Leu Glu Ser Val Arg Gly Thr Gly Leu Met Gly Leu Gly Phe Arg
165 170 175
Ser Asn Glu Ala Ala Ala Ile Lys Tyr Pro Thr Val Ile Glu Asn Met
180 185 190
Val Ser Gln Lys Ile Ile Ala Met Pro Ala Phe Ser Leu Tyr Leu Asn
195 200 205
Asp Leu Gln Thr Ser Gln Gly Ser Ile Leu Phe Gly Gly Val Asp Thr
210 215 220
Asp Lys Phe His Gly Gly Leu Ala Thr Leu Pro Phe Gln Ser Leu Pro
225 230 235 240
Ala Ser Val Ser Arg Thr Ser Asp Ile Val Met Tyr Ala Val Gln Leu
245 250 255
Asn Gly Leu Lys Val Ser Gly Val Asp Thr Pro Ser Val Asn Ala Thr
260 265 270
Ala Val Leu Asp Ser Gly Ser Thr Ile Ser Leu Leu Pro Gly Asp Val
275 280 285
Val Gln Ala Val Trp Lys Glu Phe Gly Val Met Asn Val Gln Gly Val
290 295 300
Pro Asn Pro Phe Val Asp Cys Ala Lys Ala Asn Leu Lys Asp Thr Ser
305 310 315 320
Leu Ser Phe Gln Phe Thr Asn Lys Thr Ile Gln Val Pro Ile Asp Glu
325 330 335
Met Val Ile Asn Asn Leu Ala Ser Val Gln Asp Gln Ile Phe Ser Asp
340 345 350
Pro Thr Leu Lys Gln Val Phe Lys Gly Trp Ser Gly Val Cys Thr Phe
355 360 365
Gly Met Ala Ser Thr Ser Asp Phe Gly Ile Ser Ser Asp Gln Phe Val
370 375 380
Leu Leu Gly Asp Thr Phe Leu Arg Ser Ala Tyr Val Val Tyr Asp Leu
385 390 395 400
Gln Asn Gln Gln Val Gly Ile Ala Gln Ala Thr Leu Asn Ser Thr Ser
405 410 415
Ser Ser Ile Ile Glu Phe Gln Ala Gly Ser Lys Ser Ile Pro Gly Pro
420 425 430
Val Ser Thr Gly Ser Asp Asp Ser Ser Ser Asp Asp Gly Asp Ser Ser
435 440 445
Gly Lys Asp Asn Ala Gly Val Ala Leu His Pro Ala Phe Ser Ala Ala
450 455 460
Ile Ala Gly Thr Leu Phe Val Thr Leu Ser Met Ile Met Thr Ala Leu
465 470 475 480
<210> 533
<211> 461
<212> PRT
<213> Fusarium pseudograminearum
<400> 533
Met Arg Thr Phe Thr Ile Leu Ala Ser Leu Thr Ile Gly Ala Ser Ala
1 5 10 15
Gly Thr Val Ser Ala Pro Phe Val Lys His Gly Phe His Tyr Gly Ala
20 25 30
Ser Ile Asp Lys Arg Asp Thr Leu Asn Leu Glu Ala Leu Asn Asn Ile
35 40 45
Thr Gly Gly Gly Tyr Tyr Ala Glu Phe Gln Ile Gly Thr Pro Pro Gln
50 55 60
Asn Ile Ser Phe Leu Leu Asp Thr Gly Ser Ser Asp Thr Trp Val Asn
65 70 75 80
Ser Asn Asn Thr Asp Leu Cys His Ser Gln Ala Ala Gln Lys Lys Asn
85 90 95
Gly Tyr Cys Met Thr Thr Phe Lys Pro Glu Asn Ser Arg Thr Phe Lys
100 105 110
Val Val Asp Arg Asp Gly Phe Asp Ile Arg Tyr Leu Asp Thr Arg Arg
115 120 125
Ile Glu Gly Asp Tyr Phe Asn Asp Thr Val Thr Ile Asp Gly Lys Thr
130 135 140
Val Lys Gln Gln Gln Leu Gly Leu Ala Ile Lys Ser Val Arg Pro Thr
145 150 155 160
Gly Ile Met Gly Leu Gly Phe Ser Ala Asn Val Ala Thr Asn Lys Ser
165 170 175
Tyr Pro Ala Ile Val Asp Asn Met Val Ser Gln Gly Leu Ile Asp Thr
180 185 190
Ala Thr Tyr Ser Leu Trp Leu Asn Asp Leu Asp Ser Asp Gln Gly Thr
195 200 205
Ile Leu Phe Gly Gly Ile Asp Thr Lys Arg Phe Tyr Gly Lys Leu Ala
210 215 220
Thr Leu Pro Leu Leu Pro Glu Ser Thr Lys Ser Leu Asn Val Thr Ser
225 230 235 240
Phe Ser Val Ser Leu Gln Gly Leu Asn Val Lys Thr Pro Lys Asn Ser
245 250 255
Asn Gly Ile Lys Met Asp Ser Leu Lys Lys Asn Thr Val Ala Ile Leu
260 265 270
Asp Ser Gly Ser Thr Val Cys Leu Leu Pro Asp Pro Gln Val Asn Glu
275 280 285
Ile Tyr Lys Ala Phe Asp Val Leu Ser Val Gln Asp Val Pro Ile Pro
290 295 300
Phe Val Asp Cys Gly Tyr Gly Lys Asp Lys Gly Lys Gly Ile Ser Phe
305 310 315 320
Asp Phe Glu Phe Asp Gly Lys Thr Ile Ser Ile Pro Met Ser Glu Met
325 330 335
Ile Val Asn Ala Phe Pro Asp Asn Gln Asp Leu Phe Lys Asp Pro Arg
340 345 350
Leu Asp Ile Tyr Phe Lys Asp Trp Asp Gly Val Cys Met Phe Gly Ile
355 360 365
Ser Pro Ala Ser Thr Tyr Gly Ile Gly Thr Ser Lys Phe Ala Ile Leu
370 375 380
Gly Asp Thr Phe Leu Arg Ser Ala Tyr Val Val Tyr Asp Leu Ala Asn
385 390 395 400
Glu Gln Val Gly Ile Ala Glu Ala Tyr His Ser Asn Asn Glu Ser Ser
405 410 415
Val Val Glu Leu Lys Ala Asn Ser Lys Leu Pro Asp Val Ser Gly Val
420 425 430
Pro Glu Pro Gln Asn Ala Ala Gly His Val Ser Pro Ala Ser Leu Val
435 440 445
Thr Leu Ile Val Ala Thr Gly Ile Ala Leu Thr Leu Leu
450 455 460
<210> 534
<211> 482
<212> PRT
<213> 玉米赤霉
<400> 534
Met Arg Thr Phe Thr Ile Leu Ala Ser Leu Thr Ile Gly Ala Ser Ala
1 5 10 15
Gly Thr Val Ser Ala Pro Leu Val Lys His Gly Phe His Tyr Gly Ala
20 25 30
Ser Ile Asp Lys Arg Asp Thr Leu Asn Leu Glu Ala Leu Asn Asn Ile
35 40 45
Thr Gly Gly Gly Tyr Tyr Ala Glu Phe Gln Ile Gly Thr Pro Pro Gln
50 55 60
Asn Ile Ser Phe Leu Leu Asp Thr Gly Ser Ser Asp Thr Trp Val Asn
65 70 75 80
Ser Asn Asn Thr Asp Leu Cys His Ser Gln Ala Ala Gln Lys Lys Asn
85 90 95
Gly Tyr Cys Met Thr Thr Phe Lys Pro Glu Lys Ser Arg Thr Phe Lys
100 105 110
Val Val Asp Arg Asp Gly Phe Asp Ile Arg Tyr Leu Asp Thr Arg Arg
115 120 125
Ile Glu Gly Asp Tyr Phe Asn Asp Thr Val Thr Ile Asp Gly Lys Thr
130 135 140
Val Lys Gln Gln Gln Leu Gly Leu Ala Ile Lys Ser Val Arg Pro Thr
145 150 155 160
Gly Ile Met Gly Leu Gly Phe Ser Ala Asn Val Ala Thr Asn Lys Ser
165 170 175
Tyr Pro Ala Ile Val Asp Asn Met Val Ser Gln Gly Leu Ile Asp Thr
180 185 190
Ala Ala Tyr Ser Leu Trp Leu Asn Asp Leu Asp Ser Asp Gln Gly Thr
195 200 205
Ile Leu Phe Gly Gly Ile Asp Thr Lys Arg Phe Tyr Gly Lys Leu Ala
210 215 220
Thr Leu Pro Leu Leu Pro Glu Ser Thr Lys Ser Leu Asn Val Thr Ser
225 230 235 240
Phe Ser Val Ser Leu Gln Gly Leu Asn Val Lys Thr Pro Lys Asn Ser
245 250 255
Asn Gly Ile Lys Met Asp Ser Leu Lys Lys Asn Thr Val Ala Ile Leu
260 265 270
Asp Ser Gly Ser Thr Val Cys Leu Leu Pro Asp Pro Gln Val Asn Glu
275 280 285
Ile Tyr Lys Ala Phe Asp Val Leu Ser Val Gln Asp Val Pro Ile Pro
290 295 300
Phe Ala Asp Cys Gly Tyr Gly Lys Asp Lys Gly Lys Gly Ile Ser Phe
305 310 315 320
Asp Phe Glu Phe Asp Gly Lys Thr Ile Ser Ile Pro Met Ser Glu Met
325 330 335
Ile Val Asn Ala Phe Pro Asp Asn Gln Asp Leu Phe Lys Asp Pro Arg
340 345 350
Leu Asp Ile Tyr Phe Lys Asp Trp Asp Gly Val Cys Met Phe Gly Ile
355 360 365
Ser Pro Ala Ser Thr Tyr Gly Ile Gly Thr Ser Lys Phe Ala Ile Leu
370 375 380
Gly Asp Thr Phe Leu Arg Ser Ala Tyr Val Val Tyr Asp Leu Ala Asn
385 390 395 400
Glu Gln Val Gly Ile Ala Glu Ala Tyr Arg Ser Asn Asn Glu Ser Ser
405 410 415
Val Val Glu Leu Lys Ala Asn Ser Lys Leu Pro Asp Val Ser Gly Val
420 425 430
Pro Glu Pro Glu Ser Ala Ala Gly His Val Ser Pro Ala Ser Leu Val
435 440 445
Thr Leu Ile Val Ala Ala Gly Ile Thr Leu Thr Leu Leu Lys Asp Leu
450 455 460
Arg His Leu Cys Tyr Cys Ser Tyr Cys Ser Pro Asn Cys Tyr Thr Val
465 470 475 480
Pro Ala
<210> 535
<211> 482
<212> PRT
<213> 金龟子绿僵菌
<400> 535
Met Pro Ser Leu His His Val Ala Leu Val Leu Gly Ala Val Ala Thr
1 5 10 15
Val Ser Ala Gly Thr Leu Ser Val Pro Leu Glu Arg Arg Gly Pro Ser
20 25 30
Glu Glu His Tyr Arg Leu Leu Gly Arg Ala Ile Ser Arg Arg Ala Ala
35 40 45
Asp Asn Gly Thr Ala Glu Leu Thr Ala Leu Asn Asn Ile Thr Ala Ala
50 55 60
Gly Tyr Tyr Ala Asn Phe Ala Ile Gly Thr Pro Gly Gln Asn Leu Thr
65 70 75 80
Phe Gln Leu Asp Thr Gly Ser Ser Asp Thr Trp Met Asn Ser Pro Gln
85 90 95
Thr Arg Tyr Cys Arg Ser Ala Thr Ala Gln Ser Glu Ala Gly Tyr Cys
100 105 110
Thr Thr Thr Phe Asn Pro Arg Asp Ser Lys Thr Tyr Thr Leu Val Asp
115 120 125
Gln Gly Gly Phe Asn Ile Thr Tyr Leu Asp Gln Arg Arg Ile Thr Gly
130 135 140
Asp Tyr Phe Asn Asp Thr Val Thr Ile Gly Gly His Glu Ile Ala Asn
145 150 155 160
Gln Gln Leu Gly Leu Ala Ile Ser Ser Thr Arg Pro Thr Gly Ile Met
165 170 175
Gly Leu Gly Phe Ser Ile Asn Val Ala Thr Pro Asn Lys Tyr Pro Thr
180 185 190
Ile Ile Asp Asn Met Val Ser Glu Gly Val Ile Ala His Pro Ser Phe
195 200 205
Ser Leu Tyr Leu Asn Asp Leu Asp Ala Thr Ser Gly Thr Ile Leu Phe
210 215 220
Gly Gly Ile Asp Ser Ala Lys Phe Leu Gly Ser Leu Ala Thr Leu Pro
225 230 235 240
Ile Lys Pro Met Pro Asn Ala Glu Asp Thr Asn Val Thr Ser Tyr Ala
245 250 255
Val Ala Ile Lys Ser Val Ser Ala Thr Gly Val Lys Val Pro Ser Val
260 265 270
Ala Pro Gln Ser Val Gly Ile Leu Asp Ser Gly Ser Thr Ile Ser Leu
275 280 285
Ile Pro Asp Ser Leu Val Lys Pro Ile Gln Asp Lys Phe Gly Val Ile
290 295 300
Val Val Gln Ile Gln Gly Gln Ala Ala Pro Pro Met Ile Asp Cys Ala
305 310 315 320
Trp Lys Gly Ser Lys Gly Asp Gly Ile Leu Ile Ser Phe Glu Phe Asp
325 330 335
Asn Lys Thr Ile Lys Val Pro Ile Ala Asp Met Ala Ile Asp Ser Leu
340 345 350
Pro Asp Glu Leu Gln Gln Ile Leu Lys Ser Asn Asp Ala Pro Ser Gly
355 360 365
Phe Arg Ser Trp Thr Arg Ala Cys Leu Phe Gly Leu Gly Gly Ser Ser
370 375 380
Ala Phe Gly Val Arg Asp Asp Gln Phe Tyr Leu Leu Gly Asp Thr Phe
385 390 395 400
Leu Arg Ser Ala Tyr Val Ala Tyr Asp Met Ala Asn Gln Gln Ile Gly
405 410 415
Leu Ala Gln Ser Asn Pro Asn Ala Thr Gly Ser Asn Val Val Glu Ile
420 425 430
Gln Lys Asp Ala Lys Ser Phe Pro Asn Val Gln Gly Ser Asp Ala Pro
435 440 445
Gly Pro Gly Glu Lys Ala Ser Ala Ser Gly Arg Leu Ala Pro Ser Ser
450 455 460
Ser Val Ala Ala Thr Leu Leu Leu Gly Val Ala Val Val Phe Ser Ala
465 470 475 480
Leu Leu
<210> 536
<211> 529
<212> PRT
<213> 粗糙链孢霉
<400> 536
Met Lys Ser Thr Leu Ala Thr Leu Leu Ala Leu Ala Ser Val Ala Val
1 5 10 15
Ala Glu Asn Gly Val Val Asn Phe Pro Leu Asn Arg Gly Val Pro His
20 25 30
Phe Arg Val Gly Asn Val Arg Gln Asn Val Lys Arg Asp Thr Tyr Ser
35 40 45
Gln Ala Leu Ile Asn Asn Ile Thr Gly Gly Ala Tyr Tyr Ala Glu Val
50 55 60
Thr Val Gly Thr Pro Gly Gln Lys Val Ser Val Val Leu Asp Thr Gly
65 70 75 80
Ser Ser Asp Leu Trp Val Val Ser Tyr Lys Ala Asp Leu Cys Thr Asp
85 90 95
Pro Ser Ile Gln Arg Gln Trp Gly Asp Ser Cys Asp Lys Thr Tyr Asn
100 105 110
Pro Thr Lys Ser Ser Ser Tyr Lys Val Leu Glu Glu Asp Ser Phe Glu
115 120 125
Ile Arg Tyr Leu Asp Asn Ser Thr Ala Ala Gly Asp Tyr Ile Thr Asp
130 135 140
Asp Leu Asn Ile Gly Gly Ala Thr Ile Lys Ser Leu Gln Met Gly Tyr
145 150 155 160
Ala Thr Lys Thr Val Arg Gly Ala Gly Ile Leu Gly Val Gly Tyr Ser
165 170 175
Ser Asn Val Ala Ser Gln Gln Arg Tyr Pro Asn Leu Ile Asp Gln Phe
180 185 190
Val Ala Gln Lys Leu Ile Thr Thr Lys Ala Tyr Ser Leu Tyr Leu Asn
195 200 205
Asp Arg Arg Ser Asp Thr Gly Ser Ile Leu Phe Gly Gly Ile Asp Lys
210 215 220
Asp Lys Phe Ile Gly Asp Leu Ser Ile Leu Pro Ile Tyr Leu Ala Lys
225 230 235 240
Gly Gln Ala Glu Pro Ile His Phe Glu Val Glu Met Gln Ser Val Ser
245 250 255
Leu Ala Leu Thr Lys Asn Gly Lys Thr Thr Lys Ile Ile Ser Thr Asp
260 265 270
Pro Ser Leu Ser Gln Thr Ser Thr Ile Ala Ile Leu Asp Ser Gly Thr
275 280 285
Thr Leu Ser Tyr Leu Pro Ser Lys Ile Thr Asp Gln Ile His Thr Lys
290 295 300
Leu Ser Val Tyr Val Asp Glu Ile Trp Thr Gly Leu Thr Phe Ile Asp
305 310 315 320
Cys Gln Tyr Leu Thr Ser Asn Pro Asp Leu Arg Leu Ser Phe Thr Phe
325 330 335
Gly Ala Asn Ala Thr Ile Ser Val Pro Val Trp Glu Leu Val Leu Asp
340 345 350
Leu Leu Gly Glu Ser Gln Ser Glu Leu Pro Phe Lys Met Pro Phe Lys
355 360 365
Asn Ala Cys Ile Phe Gly Ile Gln Ser Thr Ala Gly Phe Gln Glu Asp
370 375 380
Asn Phe Asp Glu Asp Trp Ala Leu Leu Gly Glu Thr Phe Leu Arg Ser
385 390 395 400
Ala Tyr Val Val Tyr Asp Leu Thr His His Gln Ile Gly Ile Ala Gln
405 410 415
Ala Asn Leu Asn Ser Thr Thr Thr Asp Ile Val Glu Leu Ser Gly Ala
420 425 430
Asp Gly Gly Leu Pro Thr Gly Leu Thr Gly Val Lys Glu Gln Gln Thr
435 440 445
Ser Asn Asp Pro Ser Gly Asn Ala Gly Ser Gly Ser Gly Ser Ser Thr
450 455 460
Asp Lys Asp Gly Ala Lys Glu Thr Glu Thr Val Thr Ala Gly Ser Thr
465 470 475 480
Ala Ala Thr Gly Thr Ala Ala Ser Gly Ala Lys Glu Thr Asp Ser Ala
485 490 495
Ala Ala Gly Leu Ser Ala Arg Gly Gly Ala Val Gly Ala Leu Ala Val
500 505 510
Ala Ser Leu Thr Gly Phe Leu Ala Leu Val Gly Gly Ala Val Val Ala
515 520 525
Leu
<210> 537
<211> 566
<212> PRT
<213> 嗜热毁丝霉
<400> 537
Met Lys Pro Ser Ser Ala Ile Leu Leu Ala Leu Ala Pro Gly Ser Ser
1 5 10 15
Ser Lys Asn Val Val Glu Phe Ser Val Ser Arg Gly Leu Pro Gly Asn
20 25 30
Arg Thr Pro Leu Ser Phe Pro Pro Leu Thr Arg Arg Glu Thr Tyr Ser
35 40 45
Glu Arg Leu Ile Asn Asn Ile Ala Gly Gly Gly Tyr Tyr Val Gln Val
50 55 60
Gln Val Gly Thr Pro Pro Gln Asn Leu Thr Met Leu Leu Asp Thr Gly
65 70 75 80
Ser Ser Asp Ala Trp Val Leu Ser His Glu Ala Asp Leu Cys Ile Ser
85 90 95
Pro Ala Leu Gln Asp Phe Tyr Gly Met Pro Cys Thr Asp Thr Tyr Asp
100 105 110
Pro Ser Lys Ser Ser Ser Lys Lys Met Val Glu Glu Gly Gly Phe Lys
115 120 125
Ile Thr Tyr Leu Asp Gly Gly Thr Ala Ser Gly Asp Tyr Ile Thr Asp
130 135 140
His Phe Thr Ile Gly Gly Val Thr Val Gln Ser Leu Gln Met Ala Cys
145 150 155 160
Val Thr Lys Ala Val Arg Gly Thr Gly Ile Leu Gly Leu Gly Phe Ser
165 170 175
Ile Ser Glu Arg Ala Ser Thr Lys Tyr Pro Asn Ile Ile Asp Glu Met
180 185 190
Tyr Ser Gln Gly Leu Ile Lys Ser Lys Ala Phe Ser Leu Tyr Leu Asn
195 200 205
Asp Arg Arg Ala Asp Ser Gly Thr Leu Leu Phe Gly Gly Ile Asp Thr
210 215 220
Asp Lys Phe Ile Gly Pro Leu Gly Val Leu Pro Leu His Lys Pro Pro
225 230 235 240
Gly Asp Arg Asp Tyr Ser Ser Phe Glu Val Asn Phe Thr Ser Val Ser
245 250 255
Leu Thr Tyr Thr Asn Gly Ser Arg His Thr Ile Pro Thr Ala Ile Leu
260 265 270
Asn His Pro Ala Pro Ala Val Leu Asp Ser Gly Thr Thr Leu Ser Tyr
275 280 285
Leu Pro Asp Glu Leu Ala Asp Pro Ile Asn Thr Ala Leu Asp Thr Phe
290 295 300
Tyr Asp Asp Arg Leu Gln Met Thr Leu Ile Asp Cys Ser His Pro Leu
305 310 315 320
Leu Arg Thr Asp Pro Asp Phe His Leu Ala Phe Thr Phe Thr Pro Thr
325 330 335
Thr Ser Ile Thr Val Pro Leu Gly Asp Leu Val Leu Asp Ile Leu Pro
340 345 350
Pro Thr Tyr Pro Gln Ser Asn Ser Asn Asn Asn Asn Glu Val Glu Asp
355 360 365
Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp Lys Val Pro Pro
370 375 380
Ala Thr Glu Arg Arg Trp Cys Val Phe Gly Ile Gln Ser Thr Thr Arg
385 390 395 400
Phe Ala Ala Ser Ser Gly Gln Ser Glu Ala Asn Phe Thr Leu Leu Gly
405 410 415
Asp Thr Phe Leu Arg Ser Ala Tyr Val Val Tyr Asp Leu Ser His Tyr
420 425 430
Gln Ile Gly Leu Ala Gln Ala Asn Leu Asn Ser Ser Ser Ser Ser Thr
435 440 445
Asn Thr Asn Thr Ile Val Glu Leu Thr Ala Asp Asn His Asp Asp Gly
450 455 460
Ala Ser Glu Arg Gly Glu Gly Ala Gly Ala Gly Ala Asp Ala Gly Thr
465 470 475 480
Arg Thr Val Ile Ala Gly Gly Leu Pro Ser Gly Leu Met Gly Val Glu
485 490 495
Ala Gln Gln Thr Thr Phe Thr Pro Thr Ala Thr Ala Asn Gly His Pro
500 505 510
Gly Tyr Gly Gly Gly Pro Gly Gly Ser Thr Arg Pro Gly Ser Glu Arg
515 520 525
Asn Ala Ala Ala Gly Gly Phe Thr Ala Val Arg Thr Gly Leu Leu Gly
530 535 540
Glu Leu Val Gly Val Ala Ala Val Thr Ala Leu Phe Ile Leu Leu Gly
545 550 555 560
Gly Ala Leu Ile Ala Val
565
<210> 538
<211> 249
<212> PRT
<213> 构巢曲霉
<400> 538
Met Lys Thr Ala Val Lys Thr Thr Ala Leu Leu Ser Leu Leu Ser Thr
1 5 10 15
Ala Met Ala Asp Lys Ala Ile Val Gly Gly Asp Asp Ala Glu Ile Thr
20 25 30
Glu Tyr Pro Tyr Gln Ile Ala Leu Leu Ser Gly Gly Ser Leu Ile Cys
35 40 45
Gly Gly Ser Ile Ile Ser Ser Lys Tyr Val Val Thr Ala Gly His Cys
50 55 60
Thr Asp Gly Ala Ser Ala Ser Ser Leu Ser Ile Arg Ala Gly Ser Thr
65 70 75 80
Tyr His Asp Lys Gly Gly Thr Val Val Asp Val Glu Ala Ile Thr Val
85 90 95
His Pro Glu Tyr Asn Ala Asn Thr Val Asp Asn Asp Ile Ser Ile Leu
100 105 110
Glu Leu Ala Glu Glu Leu Gln Phe Gly Asp Gly Ile Lys Ala Ile Asp
115 120 125
Leu Pro Ser Ser Ser Ser Leu Pro Ser Glu Gly Thr Ile Gly Thr Ala
130 135 140
Thr Gly Trp Gly Ala Leu Thr Glu Gly Gly Asn Val Ser Pro Asn Leu
145 150 155 160
Gln Tyr Val Glu Val Pro Val Val Ser Lys Ser Gln Cys Ser Ser Asp
165 170 175
Tyr Ser Gly Phe Asn Glu Ile Thr Ala Ser Met Phe Cys Ala Gly Glu
180 185 190
Glu Glu Gly Gly Lys Asp Gly Cys Gln Gly Asp Ser Gly Gly Pro Phe
195 200 205
Ala Ala Asp Gly Val Leu Ile Gly Ile Thr Ser Trp Gly Asn Gly Cys
210 215 220
Ala Arg Ala Gly Tyr Pro Gly Val Tyr Ser Ser Pro Ala Tyr Phe Arg
225 230 235 240
Asp Phe Ile Gln Gln Val Thr Gly Leu
245
<210> 539
<211> 897
<212> PRT
<213> 嗜热毁丝霉
<400> 539
Met Val Arg Leu Asp Trp Ala Ala Val Leu Leu Ala Ala Thr Ala Val
1 5 10 15
Ala Lys Ala Val Thr Pro His Thr Pro Ser Phe Val Pro Gly Ala Tyr
20 25 30
Ile Val Glu Tyr Glu Glu Asp Gln Asp Ser His Ala Phe Val Asn Lys
35 40 45
Leu Gly Gly Lys Ala Ser Leu Arg Lys Asp Leu Arg Phe Lys Leu Phe
50 55 60
Lys Gly Ala Ser Ile Gln Phe Lys Asp Thr Glu Thr Ala Asp Gln Met
65 70 75 80
Val Ala Lys Val Ala Glu Met Pro Lys Val Lys Ala Val Tyr Pro Val
85 90 95
Arg Arg Tyr Pro Val Pro Asn His Val Val His Ser Thr Gly Asn Val
100 105 110
Ala Asp Glu Val Leu Val Lys Arg Gln Ala Ala Gly Asn Asp Thr Phe
115 120 125
Ser Thr His Leu Met Thr Gln Val Asn Lys Phe Arg Asp Ala Gly Ile
130 135 140
Thr Gly Lys Gly Ile Lys Ile Ala Val Ile Asp Thr Gly Ile Asp Tyr
145 150 155 160
Leu His Glu Ala Leu Gly Gly Cys Phe Gly Pro Asp Cys Leu Val Ser
165 170 175
Tyr Gly Thr Asp Leu Val Gly Asp Asp Phe Asn Gly Ser Asn Thr Pro
180 185 190
Lys Pro Asp Pro Asp Pro Ile Asp Asn Cys Gln Gly His Gly Thr His
195 200 205
Val Ala Gly Ile Ile Ala Ala Gln Thr Asn Asn Pro Phe Gly Ile Ile
210 215 220
Gly Ala Ala Thr Asp Val Thr Leu Gly Ala Tyr Arg Val Phe Gly Cys
225 230 235 240
Asn Gly Asp Thr Pro Asn Asp Val Leu Ile Ala Ala Tyr Asn Met Ala
245 250 255
Tyr Glu Ala Gly Ser Asp Ile Ile Thr Ala Ser Ile Gly Gly Pro Ser
260 265 270
Gly Trp Ser Glu Asp Pro Trp Ala Ala Val Val Thr Arg Ile Val Glu
275 280 285
Asn Gly Val Pro Cys Val Val Ser Ala Gly Asn Asp Gly Asp Ala Gly
290 295 300
Ile Phe Tyr Ala Ser Thr Ala Ala Asn Gly Lys Lys Val Thr Ala Ile
305 310 315 320
Ala Ser Val Asp Asn Ile Val Thr Pro Ala Leu Leu Ser Asn Ala Ser
325 330 335
Tyr Thr Leu Asn Gly Thr Asp Asp Phe Phe Gly Phe Thr Ala Gly Asp
340 345 350
Pro Gly Ser Trp Asp Asp Val Asn Leu Pro Leu Trp Ala Val Ser Phe
355 360 365
Asp Thr Thr Asp Pro Ala Asn Gly Cys Asn Pro Tyr Pro Asp Ser Thr
370 375 380
Pro Asp Leu Ser Gly Tyr Ile Val Leu Ile Arg Arg Gly Thr Cys Thr
385 390 395 400
Phe Val Glu Lys Ala Ser Tyr Ala Ala Ala Lys Gly Ala Lys Tyr Val
405 410 415
Met Phe Tyr Asn Asn Val Gln Gln Gly Thr Val Thr Val Ser Ala Ala
420 425 430
Glu Ala Lys Gly Ile Glu Gly Val Ala Met Val Thr Ala Gln Gln Gly
435 440 445
Glu Ala Trp Val Arg Ala Leu Glu Ala Gly Ser Glu Val Val Leu His
450 455 460
Met Lys Asp Pro Leu Lys Ala Gly Lys Phe Leu Thr Thr Thr Pro Asn
465 470 475 480
Thr Ala Thr Gly Gly Phe Met Ser Asp Tyr Thr Ser Trp Gly Pro Thr
485 490 495
Trp Glu Val Glu Val Lys Pro Gln Phe Gly Thr Pro Gly Gly Ser Ile
500 505 510
Leu Ser Thr Tyr Pro Arg Ala Leu Gly Ser Tyr Ala Val Leu Ser Gly
515 520 525
Thr Ser Met Ala Cys Pro Leu Ala Ala Ala Ile Tyr Ala Leu Leu Ile
530 535 540
Asn Thr Arg Gly Thr Lys Asp Pro Lys Thr Leu Glu Asn Leu Ile Ser
545 550 555 560
Ser Thr Ala Arg Pro Asn Leu Phe Arg Leu Asn Gly Glu Ser Leu Pro
565 570 575
Leu Leu Ala Pro Val Pro Gln Gln Gly Gly Gly Ile Val Gln Ala Trp
580 585 590
Asp Ala Ala Gln Ala Thr Thr Leu Leu Ser Val Ser Ser Leu Ser Phe
595 600 605
Asn Asp Thr Asp His Phe Lys Pro Val Gln Thr Phe Thr Ile Thr Asn
610 615 620
Thr Gly Lys Lys Ala Val Thr Tyr Ser Leu Ser Asn Val Gly Ala Ala
625 630 635 640
Thr Ala Tyr Thr Phe Ala Asp Ala Lys Ser Ile Glu Pro Ala Pro Phe
645 650 655
Pro Asn Glu Leu Thr Ala Asp Phe Ala Ser Leu Thr Phe Val Pro Lys
660 665 670
Arg Leu Thr Ile Pro Ala Gly Lys Arg Gln Thr Val Thr Val Ile Ala
675 680 685
Lys Pro Ser Glu Gly Val Asp Ala Lys Arg Leu Pro Val Tyr Ser Gly
690 695 700
Tyr Ile Ala Ile Asn Gly Ser Asp Ser Ser Ala Leu Ser Leu Pro Tyr
705 710 715 720
Leu Gly Val Val Gly Ser Leu His Ser Ala Val Val Leu Asp Ser Asn
725 730 735
Gly Ala Arg Ile Ser Leu Ala Ser Asp Asp Thr Asn Lys Pro Leu Pro
740 745 750
Ala Asn Thr Ser Phe Val Leu Pro Pro Ala Gly Phe Pro Asn Asp Thr
755 760 765
Ser Tyr Ala Asn Ser Thr Asp Leu Pro Lys Leu Val Val Asp Leu Ala
770 775 780
Met Gly Ser Ala Leu Leu Arg Ala Asp Val Val Pro Leu Ser Gly Gly
785 790 795 800
Ala Ala Thr Ala Thr Ala Arg Leu Thr Arg Thr Val Phe Gly Thr Arg
805 810 815
Thr Ile Gly Gln Pro Tyr Gly Leu Pro Ala Arg Tyr Asn Pro Arg Gly
820 825 830
Thr Phe Glu Tyr Ala Trp Asp Gly Arg Leu Asp Asp Gly Ser Tyr Ala
835 840 845
Pro Ala Gly Arg Tyr Arg Phe Ala Val Lys Ala Leu Arg Ile Phe Gly
850 855 860
Asp Ala Lys Arg Ala Arg Glu Tyr Asp Ala Ala Glu Thr Val Glu Phe
865 870 875 880
Asn Ile Glu Tyr Leu Pro Gly Pro Ser Ala Lys Phe Arg Arg Arg Leu
885 890 895
Phe
<210> 540
<211> 534
<212> PRT
<213> 嗜热毁丝霉
<400> 540
Met Arg Gly Leu Val Ala Phe Ser Leu Ala Ala Cys Val Ser Ala Ala
1 5 10 15
Pro Ser Phe Lys Thr Glu Thr Ile Asn Gly Glu His Ala Pro Ile Leu
20 25 30
Ser Ser Ser Asn Ala Glu Val Val Pro Asn Ser Tyr Ile Ile Lys Phe
35 40 45
Lys Lys His Val Asp Glu Ser Ser Ala Ser Ala His His Ala Trp Ile
50 55 60
Gln Asp Ile His Thr Ser Arg Glu Lys Val Arg Gln Asp Leu Lys Lys
65 70 75 80
Arg Gly Gln Val Pro Leu Leu Asp Asp Val Phe His Gly Leu Lys His
85 90 95
Thr Tyr Lys Ile Gly Gln Glu Phe Leu Gly Tyr Ser Gly His Phe Asp
100 105 110
Asp Glu Thr Ile Glu Gln Val Arg Arg His Pro Asp Val Glu Tyr Ile
115 120 125
Glu Arg Asp Ser Ile Val His Thr Met Arg Val Thr Glu Glu Thr Cys
130 135 140
Asp Gly Glu Leu Glu Lys Ala Ala Pro Trp Gly Leu Ala Arg Ile Ser
145 150 155 160
His Arg Asp Thr Leu Gly Phe Ser Thr Phe Asn Lys Tyr Leu Tyr Ala
165 170 175
Ala Glu Gly Gly Glu Gly Val Asp Ala Tyr Val Ile Asp Thr Gly Thr
180 185 190
Asn Ile Glu His Val Asp Phe Glu Gly Arg Ala Lys Trp Gly Lys Thr
195 200 205
Ile Pro Ala Gly Asp Ala Asp Val Asp Gly Asn Gly His Gly Thr His
210 215 220
Cys Ser Gly Thr Ile Ala Gly Lys Lys Tyr Gly Val Ala Lys Lys Ala
225 230 235 240
Asn Val Tyr Ala Val Lys Val Leu Arg Ser Asn Gly Ser Gly Thr Met
245 250 255
Ala Asp Val Val Ala Gly Val Glu Trp Ala Ala Lys Ser His Leu Glu
260 265 270
Gln Val Gln Ala Ala Lys Asp Gly Lys Arg Lys Gly Phe Lys Gly Ser
275 280 285
Val Ala Asn Met Ser Leu Gly Gly Gly Lys Thr Arg Ala Leu Asp Asp
290 295 300
Thr Val Asn Ala Ala Val Ser Val Gly Ile His Phe Ala Val Ala Ala
305 310 315 320
Gly Asn Asp Asn Ala Asp Ala Cys Asn Tyr Ser Pro Ala Ala Ala Glu
325 330 335
Lys Ala Val Thr Val Gly Ala Ser Ala Ile Asp Asp Ser Arg Ala Tyr
340 345 350
Phe Ser Asn Tyr Gly Lys Cys Thr Asp Ile Phe Ala Pro Gly Leu Ser
355 360 365
Ile Leu Ser Thr Trp Ile Gly Ser Lys Tyr Ala Thr Asn Thr Ile Ser
370 375 380
Gly Thr Ser Met Ala Ser Pro His Ile Ala Gly Leu Leu Ala Tyr Tyr
385 390 395 400
Leu Ser Leu Gln Pro Ala Thr Asp Ser Glu Tyr Ser Val Ala Pro Ile
405 410 415
Thr Pro Glu Lys Met Lys Ser Asn Leu Leu Lys Ile Ala Thr Gln Asp
420 425 430
Ala Leu Thr Asp Ile Pro Asp Glu Thr Pro Asn Leu Leu Ala Trp Asn
435 440 445
Gly Gly Gly Cys Asn Asn Tyr Thr Ala Ile Val Glu Ala Gly Gly Tyr
450 455 460
Lys Ala Lys Lys Lys Thr Thr Thr Asp Lys Val Asp Ile Gly Ala Ser
465 470 475 480
Val Ser Glu Leu Glu Lys Leu Ile Glu His Asp Phe Glu Val Ile Ser
485 490 495
Gly Lys Val Val Lys Gly Val Ser Ser Phe Ala Asp Lys Ala Glu Lys
500 505 510
Phe Ser Glu Lys Ile His Glu Leu Val Asp Glu Glu Leu Lys Glu Phe
515 520 525
Leu Glu Asp Ile Ala Ala
530
<210> 541
<211> 497
<212> PRT
<213> 黑曲霉
<400> 541
Met Lys Gly Ile Leu Gly Leu Ser Leu Leu Pro Leu Leu Thr Ala Ala
1 5 10 15
Ser Pro Val Phe Val Asp Ser Ile His Asn Glu Ala Ala Pro Ile Leu
20 25 30
Ser Ala Thr Asn Ala Lys Glu Val Pro Asp Ser Tyr Ile Val Val Phe
35 40 45
Lys Lys His Val Thr Ser Glu Leu Ala Ser Ala His His Ser Trp Val
50 55 60
Gln Asp Ile His Asp Ser Gln Ser Glu Arg Thr Glu Leu Lys Lys Arg
65 70 75 80
Ser Leu Phe Gly Leu Gly Asp Glu Val Tyr Leu Gly Leu Lys Asn Thr
85 90 95
Phe Asp Ile Ala Gly Ser Leu Ile Gly Tyr Ser Gly His Phe His Glu
100 105 110
Asp Val Ile Glu Gln Val Arg Arg His Pro Asp Val Asp Tyr Ile Glu
115 120 125
Arg Asp Ser Glu Val His Thr Met Glu Gly Ala Thr Glu Lys Asn Ala
130 135 140
Pro Trp Gly Leu Ala Arg Ile Ser His Arg Asp Ser Leu Thr Phe Gly
145 150 155 160
Asn Phe Asn Lys Tyr Leu Tyr Ala Ser Glu Gly Gly Glu Gly Val Asp
165 170 175
Ala Tyr Thr Ile Asp Thr Gly Ile Asn Val Asp His Val Asp Phe Glu
180 185 190
Gly Arg Ala Thr Trp Gly Lys Thr Ile Pro Thr Asn Asp Glu Asp Leu
195 200 205
Asp Gly Asn Gly His Gly Thr His Cys Ser Gly Thr Met Ala Gly Lys
210 215 220
Lys Tyr Gly Val Ala Lys Lys Ala Asn Leu Tyr Ala Val Lys Val Leu
225 230 235 240
Arg Ser Ser Gly Ser Gly Thr Met Ser Asp Val Val Ser Gly Val Glu
245 250 255
Tyr Ala Val Gln Ala His Ile Lys Lys Ala Lys Asp Ala Lys Asn Gly
260 265 270
Lys Val Lys Gly Phe Lys Gly Ser Val Ala Asn Met Ser Leu Gly Gly
275 280 285
Gly Lys Ser Lys Thr Leu Glu Asp Ala Val Asn Ala Gly Val Glu Ala
290 295 300
Gly Leu His Phe Ala Val Ala Ala Gly Asn Asp Asn Ala Asp Ala Cys
305 310 315 320
Asn Tyr Ser Pro Ala Ala Ala Glu Lys Ala Ile Thr Val Gly Ala Ser
325 330 335
Thr Leu Ala Asp Glu Arg Ala Tyr Phe Ser Asn Tyr Gly Glu Cys Thr
340 345 350
Asp Ile Phe Ala Pro Gly Leu Asn Ile Leu Ser Thr Trp Ile Gly Ser
355 360 365
Asn Tyr Ala Thr Asn Ile Ile Ser Gly Thr Ser Met Ala Ser Pro His
370 375 380
Ile Ala Gly Leu Leu Ala Tyr Phe Val Ser Leu Gln Pro Pro Ser Asp
385 390 395 400
Ser Ala Phe Ala Val Glu Glu Leu Thr Pro Ala Lys Leu Lys Lys Asp
405 410 415
Ile Ile Ala Ile Ala Thr Glu Gly Ala Leu Thr Asp Ile Pro Ser Asn
420 425 430
Thr Pro Asn Leu Leu Ala Trp Asn Gly Gly Gly Ser Glu Asn Tyr Thr
435 440 445
Asp Ile Val Gly Ser Gly Gly Tyr Lys Val Ser Ser Ala Lys Asn Arg
450 455 460
Ile Glu Asp Arg Ile Glu Gly Leu Val His Lys Ala Glu Glu Leu Leu
465 470 475 480
Thr Glu Glu Leu Gly Ala Ile Tyr Ser Glu Ile Gln Asp Ala Val Val
485 490 495
Ala
<210> 542
<211> 495
<212> PRT
<213> 米曲霉
<400> 542
Met Arg Gly Ile Leu Gly Leu Ser Leu Leu Pro Leu Leu Ala Ala Ala
1 5 10 15
Ser Pro Val Ala Val Asp Ser Ile His Asn Gly Ala Ala Pro Ile Leu
20 25 30
Ser Ala Ser Asn Ala Lys Glu Val Pro Asp Ser Tyr Ile Val Val Phe
35 40 45
Lys Lys His Val Ser Ala Glu Thr Ala Ala Ala His His Thr Trp Val
50 55 60
Gln Asp Ile His Asp Ser Met Thr Gly Arg Ile Asp Leu Lys Lys Arg
65 70 75 80
Ser Leu Phe Gly Phe Ser Asp Asp Leu Tyr Leu Gly Leu Lys Asn Thr
85 90 95
Phe Asp Ile Ala Gly Ser Leu Ala Gly Tyr Ser Gly His Phe His Glu
100 105 110
Asp Val Ile Glu Gln Val Arg Arg His Pro Asp Val Glu Tyr Ile Glu
115 120 125
Lys Asp Thr Glu Val His Thr Met Glu Glu Thr Thr Glu Lys Asn Ala
130 135 140
Pro Trp Gly Leu Ala Arg Ile Ser His Arg Asp Ser Leu Ser Phe Gly
145 150 155 160
Thr Phe Asn Lys Tyr Leu Tyr Ala Ser Glu Gly Gly Glu Gly Val Asp
165 170 175
Ala Tyr Thr Ile Asp Thr Gly Ile Asn Ile Glu His Val Asp Phe Glu
180 185 190
Asp Arg Ala His Trp Gly Lys Thr Ile Pro Ser Asn Asp Glu Asp Ala
195 200 205
Asp Gly Asn Gly His Gly Thr His Cys Ser Gly Thr Ile Ala Gly Lys
210 215 220
Lys Tyr Gly Val Ala Lys Lys Ala Asn Ile Tyr Ala Val Lys Val Leu
225 230 235 240
Arg Ser Ser Gly Ser Gly Thr Met Ser Asp Val Val Leu Gly Val Glu
245 250 255
Trp Ala Val Gln Ser His Leu Lys Lys Ala Lys Asp Ala Lys Asp Gly
260 265 270
Lys Val Lys Gly Phe Lys Gly Ser Val Ala Asn Met Ser Leu Gly Gly
275 280 285
Gly Lys Ser Arg Thr Leu Glu Ala Ala Val Asn Ala Gly Val Glu Ala
290 295 300
Gly Leu His Phe Ala Val Ala Ala Gly Asn Asp Asn Ala Asp Ala Cys
305 310 315 320
Asn Tyr Ser Pro Ala Ala Ala Glu Asn Ala Ile Thr Val Gly Ala Ser
325 330 335
Thr Leu Gln Asp Glu Arg Ala Tyr Phe Ser Asn Tyr Gly Lys Cys Thr
340 345 350
Asp Ile Phe Ala Pro Gly Leu Asn Ile Leu Ser Thr Trp Thr Gly Ser
355 360 365
Lys His Ala Val Asn Thr Ile Ser Gly Thr Ser Met Ala Ser Pro His
370 375 380
Ile Ala Gly Leu Leu Ala Tyr Phe Val Ser Leu Gln Pro Ala Gln Asp
385 390 395 400
Ser Ala Phe Ala Val Asp Glu Leu Thr Pro Ala Lys Leu Lys Lys Asp
405 410 415
Ile Ile Ser Ile Ala Thr Gln Gly Ala Leu Thr Asp Ile Pro Ser Asp
420 425 430
Thr Pro Asn Leu Leu Ala Trp Asn Gly Gly Gly Ala Asp Asn Tyr Thr
435 440 445
Gln Ile Val Ala Lys Gly Gly Tyr Lys Ala Gly Ser Asp Asn Leu Lys
450 455 460
Asp Arg Phe Asp Gly Leu Val Asn Lys Ala Glu Lys Leu Leu Ala Glu
465 470 475 480
Glu Leu Gly Ala Ile Tyr Ser Glu Ile Gln Gly Ala Val Val Ala
485 490 495
<210> 543
<211> 494
<212> PRT
<213> 产黄青霉
<400> 543
Met Lys Gly Phe Leu Ser Leu Thr Leu Leu Pro Leu Leu Val Ala Ala
1 5 10 15
Ser Pro Val Ala Val Asn Ser Ile His Asn Asp Ala Ala Pro Ile Leu
20 25 30
Ser Ser Met Thr Ser Lys Asp Ile Pro Asp Ser Tyr Ile Val Val Phe
35 40 45
Lys Lys His Val Asp Pro Ser Ser Ala Ser Ala His Gln Ser Trp Leu
50 55 60
Gln Glu Val His Thr Ala His Thr Gly Arg Met Glu Leu Lys Lys Arg
65 70 75 80
Ser Leu Phe Gly Phe Asp Phe Glu Ala Phe Met Gly Leu Lys His Thr
85 90 95
Phe His Ile Ala Gly Ser Leu Leu Gly Tyr Ala Gly His Phe His Glu
100 105 110
Asp Val Ile Glu Gln Ile Arg Arg His Pro Asp Val Asp Tyr Ile Glu
115 120 125
Lys Asp Ser Glu Val Arg Thr Met Ser Glu Gly Ser Val Glu Lys Asn
130 135 140
Ala Pro Trp Gly Leu Ala Arg Ile Ser His Arg Glu Ser Leu Ser Phe
145 150 155 160
Gly Asn Phe Asn Lys Tyr Leu Tyr Ala Glu Glu Gly Gly Glu Gly Val
165 170 175
Asp Ala Tyr Val Ile Asp Thr Gly Ala Asn Val Lys His Val Asp Phe
180 185 190
Glu Gly Arg Ala Asn Trp Gly Lys Thr Ile Pro Gln Gly Asp Ala Asp
195 200 205
Glu Asp Gly Asn Gly His Gly Thr His Cys Ser Gly Thr Ile Ala Gly
210 215 220
Lys Lys Phe Gly Val Ala Lys Lys Ala Asn Val Tyr Ala Val Lys Val
225 230 235 240
Leu Arg Ser Asn Gly Ser Gly Thr Met Ser Asp Val Val Lys Gly Val
245 250 255
Glu Trp Ala Ala Glu Ala His Ile Lys Lys Ser Lys Lys Gly Asp Lys
260 265 270
Lys Phe Lys Gly Ser Val Ala Asn Met Ser Leu Gly Gly Gly Ser Ser
275 280 285
Arg Thr Leu Asp Leu Ala Val Asn Ala Ala Val Asp Ala Gly Ile His
290 295 300
Phe Ala Val Ala Ala Gly Asn Asp Asn Ala Asp Ala Cys Asn Tyr Ser
305 310 315 320
Pro Ala Ala Ala Glu Lys Ala Ile Thr Val Gly Ala Ser Thr Leu Ala
325 330 335
Asp Glu Arg Ala Tyr Phe Ser Asn Tyr Gly Lys Cys Thr Asp Ile Phe
340 345 350
Ala Pro Gly Leu Asn Ile Leu Ser Thr Trp Val Gly Ser Asp His Ala
355 360 365
Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Ser Pro His Ile Ala Gly
370 375 380
Leu Leu Ala Tyr Tyr Val Ser Leu Ala Pro Ala Lys Asp Ser Ala Tyr
385 390 395 400
Ala Val Ala Asp Val Thr Pro Lys Gln Leu Lys Ala Ala Leu Ile Ser
405 410 415
Val Ala Thr Glu Gly Thr Leu Thr Asp Ile Pro Ser Asp Thr Pro Asn
420 425 430
Leu Leu Ala Trp Asn Gly Gly Gly Ser Ala Asn Tyr Thr Lys Ile Leu
435 440 445
Ala Asp Gly Gly Tyr Lys Ala His Asn Ala Glu Thr Thr Val Glu Asp
450 455 460
Arg Ile Gly Gly Ile Ile Asp Ser Ala Glu Lys Ala Phe His Lys Glu
465 470 475 480
Leu Gly Ala Ile Tyr Ser Glu Ile Lys Asp Ala Val Ser Ala
485 490
<210> 544
<211> 477
<212> PRT
<213> 构巢曲霉
<400> 544
Met Lys Gly Ile Phe Gly Leu Ser Leu Leu Pro Leu Leu Val Ala Ala
1 5 10 15
Ser Pro Val Val Val Asp Ser Ile His Asn Gly Ala Ala Pro Ile Leu
20 25 30
Ser Ser Thr Asn Ala Lys Glu Val Pro Asp Ser Tyr Ile Val Val Phe
35 40 45
Lys Lys His Val Asp Pro Val Ala Ala Ser Val His His Ser Trp Val
50 55 60
Gln Asp Ile His Gly Gln Lys Ser Gly Gly Arg Ala Asp Leu Lys Lys
65 70 75 80
Arg Phe Leu Gly Leu Glu Phe Gly Phe Gly Glu Glu Ile Tyr Asp Gly
85 90 95
Leu Lys Ala Thr Phe Asn Ile Ala Gly Ser Leu Met Gly Tyr Ser Gly
100 105 110
His Phe His Glu Asp Thr Ile Glu Glu Ile Arg Lys His Pro Asp Ile
115 120 125
Glu Tyr Ile Glu Arg Asn Ser Glu Val His Thr Leu Glu Asp Thr Asp
130 135 140
Val Glu Lys Asn Ala Pro Trp Gly Leu Ala Arg Ile Ser His Arg Asp
145 150 155 160
Arg Leu Thr Phe Gly Thr Phe Asn Lys Tyr Leu Tyr Ala Ser Glu Gly
165 170 175
Gly Glu Gly Val Asp Val Tyr Thr Ile Asp Thr Gly Ile Asn Ile Glu
180 185 190
His Glu Asp Phe Glu Gly Arg Ala Phe Trp Gly Lys Thr Ile Pro Asn
195 200 205
Asn Asp Ala Asp Glu Asp Gly Asn Gly His Gly Thr His Cys Ser Gly
210 215 220
Thr Ile Ala Gly Lys Lys Tyr Gly Val Ser Lys Lys Ala Asn Ile Tyr
225 230 235 240
Ala Val Lys Val Leu Arg Ser Ser Gly Ser Gly Thr Met Ala Asp Val
245 250 255
Val Gln Gly Val Glu Trp Ala Val Glu Ser His Leu Lys Lys Ala Lys
260 265 270
Lys Gly Gly Asn Gly Phe Lys Gly Ser Val Ala Asn Met Ser Leu Gly
275 280 285
Gly Gly Lys Ser Lys Thr Leu Glu Asp Ala Val Asn Ala Gly Val Glu
290 295 300
Ala Gly Val His Phe Ala Val Ala Ala Gly Asn Asp Asn Ala Asp Ala
305 310 315 320
Cys Ser Tyr Ser Pro Ala Ala Ala Glu Lys Ala Val Thr Val Gly Ala
325 330 335
Ser Thr Leu Ala Asp Glu Arg Ala Tyr Phe Ser Asn Tyr Gly Lys Cys
340 345 350
Thr Asp Ile Phe Ala Pro Gly Leu Asn Ile Leu Ser Thr Trp Ile Gly
355 360 365
Ser Lys Ser Ala Val Asn Thr Ile Ser Gly Thr Ser Met Ala Ser Pro
370 375 380
His Ile Ala Gly Leu Leu Ala Tyr Phe Val Ser Leu Gln Pro Ser Lys
385 390 395 400
Asp Ser Ala Phe Ala Val Asp Phe Thr Pro Glu Lys Leu Lys Lys Asp
405 410 415
Ile Ile Ser Ile Ala Thr Glu Gly Val Leu Thr Asp Ile Pro Ala Asp
420 425 430
Thr Pro Asn Leu Leu Ala Trp Asn Gly Gly Gly Ser Thr Asn Tyr Ser
435 440 445
Asp Ile Ile Ala Lys Gly Gly Tyr Lys Ser Gly Ser His Val Asp Ser
450 455 460
Thr Pro Phe Lys Ala Gly Gly Ile Leu Ala Gln Ala Met
465 470 475
<210> 545
<211> 396
<212> PRT
<213> 粗糙链孢霉
<400> 545
Met Lys Leu Ser Ala Val Leu Ala Leu Leu Pro Leu Ala Met Ala Ala
1 5 10 15
Pro Ser Ala Pro Ile Asp Lys Arg Ala Pro Ile Leu Glu Ala Arg Ala
20 25 30
Gly Thr Gln Ala Val Pro Gly Lys Tyr Ile Val Lys Leu Arg Glu Thr
35 40 45
Ala Ser Asp Asp Asp Leu Asp Lys Ala Val Lys Lys Leu Gly Asn Ser
50 55 60
Lys Ala Asp His Val Tyr Lys His Ala Phe Arg Gly Phe Ala Gly Arg
65 70 75 80
Ile Asp Asp Lys Thr Leu Asp Asp Ile Arg Ser Leu Pro Glu Val Glu
85 90 95
Tyr Val Glu Gln Glu Ala Val Phe Thr Ile Asn Thr Tyr Thr Ser Gln
100 105 110
Ser Ser Val Pro Ser Trp Gly Leu Ala Arg Leu Ser Ser Lys Thr Thr
115 120 125
Gly Lys Thr Thr Tyr Val Tyr Asp Ser Ser Ala Gly Ala Gly Thr Cys
130 135 140
Ala Tyr Ile Ile Asp Thr Gly Ile Asn Thr Ala His Ser Asp Phe Gly
145 150 155 160
Gly Arg Ala Thr Trp Leu Ala Asn Tyr Ala Gly Asp Gly Ile Asn Ser
165 170 175
Asp Gly Asn Gly His Gly Thr His Val Ala Gly Thr Val Gly Gly Thr
180 185 190
Thr Tyr Gly Val Ala Lys Lys Thr Gln Leu Tyr Ala Val Lys Val Leu
195 200 205
Asp Ser Asn Gly Ser Gly Ser Asn Ser Gly Val Ile Ala Gly Met Asn
210 215 220
Phe Val Ala Gln Asp Ala Gln Ser Arg Asn Cys Pro Asn Gly Thr Val
225 230 235 240
Ala Asn Met Ser Leu Gly Gly Gly Tyr Ser Ala Ser Thr Asn Ser Ala
245 250 255
Ala Ala Ala Met Val Arg Ala Gly Val Phe Leu Ala Val Ala Ala Gly
260 265 270
Asn Asp Gly Ala Asn Ala Ala Asn Tyr Ser Pro Ala Ser Glu Pro Thr
275 280 285
Val Cys Thr Val Gly Ala Thr Thr Ser Ala Asp Ala Ile Ala Tyr Tyr
290 295 300
Ser Asn Tyr Gly Thr Ile Val Asp Ile Phe Ala Pro Gly Thr Ser Ile
305 310 315 320
Thr Ser Ala Trp Ile Gly Ser Thr Thr Ala Lys Asn Thr Ile Ser Gly
325 330 335
Thr Ser Met Ala Thr Pro His Ile Thr Gly Leu Gly Ala Tyr Leu Leu
340 345 350
Thr Leu Leu Gly Lys Lys Ser Pro Ala Ala Leu Cys Ser Tyr Ile Ala
355 360 365
Ser Thr Ala Asn Ser Gly Val Ile Ser Gly Ile Pro Arg Gly Thr Val
370 375 380
Asn Lys Leu Ala Phe Asn Gly Asn Pro Ser Ala Tyr
385 390 395
<210> 546
<211> 392
<212> PRT
<213> 嗜热毁丝霉
<400> 546
Met His Phe Ser Thr Ala Leu Leu Ala Phe Leu Pro Ala Ala Leu Ala
1 5 10 15
Ala Pro Thr Ala Glu Thr Leu Asp Lys Arg Ala Pro Ile Leu Thr Ala
20 25 30
Arg Ala Gly Gln Val Val Pro Gly Lys Tyr Ile Ile Lys Leu Arg Asp
35 40 45
Gly Ala Ser Asp Asp Val Leu Glu Ala Ala Ile Gly Lys Leu Arg Ser
50 55 60
Lys Ala Asp His Val Tyr Arg Gly Lys Phe Arg Gly Phe Ala Gly Lys
65 70 75 80
Leu Glu Asp Asp Val Leu Asp Ala Ile Arg Leu Leu Pro Glu Val Glu
85 90 95
Tyr Val Glu Glu Glu Ala Ile Phe Thr Ile Asn Ala Tyr Thr Ser Gln
100 105 110
Ser Asn Ala Pro Trp Gly Leu Ala Arg Leu Ser Ser Lys Thr Ala Gly
115 120 125
Ser Thr Thr Tyr Thr Tyr Asp Thr Ser Ala Gly Glu Gly Thr Cys Ala
130 135 140
Tyr Val Ile Asp Thr Gly Ile Tyr Thr Ser His Ser Asp Phe Gly Gly
145 150 155 160
Arg Ala Thr Phe Ala Ala Asn Phe Val Asp Ser Ser Asn Thr Asp Gly
165 170 175
Asn Gly His Gly Thr His Val Ala Gly Thr Ile Gly Gly Thr Thr Tyr
180 185 190
Gly Val Ala Lys Lys Thr Lys Leu Tyr Ala Val Lys Val Leu Gly Ser
195 200 205
Asp Gly Ser Gly Thr Thr Ser Gly Val Ile Ala Gly Ile Asn Phe Val
210 215 220
Ala Asp Asp Ala Pro Lys Arg Ser Cys Pro Lys Gly Val Val Ala Asn
225 230 235 240
Met Ser Leu Gly Gly Ser Tyr Ser Ala Ser Ile Asn Asn Ala Ala Ala
245 250 255
Ala Leu Val Arg Ser Gly Val Phe Leu Ala Val Ala Ala Gly Asn Glu
260 265 270
Asn Gln Asn Ala Ala Asn Ser Ser Pro Ala Ser Glu Ala Ser Ala Cys
275 280 285
Thr Val Gly Ala Thr Asp Arg Asn Asp Ala Lys Ala Ser Tyr Ser Asn
290 295 300
Tyr Gly Ser Val Val Asp Ile Gln Ala Pro Gly Ser Asn Ile Leu Ser
305 310 315 320
Thr Trp Ile Gly Ser Thr Ser Ala Thr Asn Thr Ile Ser Gly Thr Ser
325 330 335
Met Ala Ser Pro His Ile Ala Gly Leu Gly Ala Tyr Leu Leu Ala Leu
340 345 350
Glu Gly Ser Lys Thr Pro Ala Glu Leu Cys Asn Tyr Ile Lys Ser Thr
355 360 365
Gly Asn Ala Ala Ile Thr Gly Val Pro Ser Gly Thr Thr Asn Arg Ile
370 375 380
Ala Phe Asn Gly Asn Pro Ser Ala
385 390
<210> 547
<211> 403
<212> PRT
<213> 构巢曲霉
<400> 547
Met His Ser Phe Lys Arg Ser Leu Leu Leu Leu Gly Ala Leu Leu Pro
1 5 10 15
Ala Val Phe Gly Ala Pro Val Glu Pro Arg Arg Ala Ala Glu Lys Val
20 25 30
Pro Gly Lys Tyr Ile Val Thr Phe Lys Ser Gly Leu Asn Val Asp Gln
35 40 45
Ile Asp Ala His Thr Ser Trp Ala Ser Asn Val His Lys Arg Asn Leu
50 55 60
Glu Arg Arg Gly Leu Ala Glu Arg Asp Gln Tyr Ser Gly Ile Glu Lys
65 70 75 80
Asn Tyr Lys Ile Asn Lys Phe Ala Ala Tyr Ser Gly Ser Phe Asp Asp
85 90 95
Ala Thr Ile Glu Glu Ile Arg Asn Ser Ala Asp Val Ala His Val Glu
100 105 110
Glu Asp Gln Ile Trp Tyr Ile Asp Ala Leu Thr Ser Gln Ser Gly Ala
115 120 125
Pro Trp Gly Leu Gly Ala Ile Ser His Lys Gly Glu Ala Ser Thr Thr
130 135 140
Tyr Val Tyr Asp Thr Ser Ala Gly Glu Gly Thr Tyr Ala Tyr Val Val
145 150 155 160
Asp Thr Gly Ile Asn Ala Asp His Glu Glu Phe Gly Gly Arg Ala Ser
165 170 175
Leu Ala Tyr Asn Ala Val Gly Gly Gln His Val Asp Ser Val Gly His
180 185 190
Gly Thr His Val Ala Gly Thr Ile Gly Gly Glu Thr Tyr Gly Val Ser
195 200 205
Lys Lys Ala Asn Leu Leu Ser Val Lys Val Phe Gln Gly Glu Ser Ser
210 215 220
Ser Thr Ser Ile Ile Leu Asp Gly Phe Asn Trp Ala Ala Asn Asp Ile
225 230 235 240
Val Ser Lys Gly Arg Thr Gly Lys Ser Ala Ile Asn Met Ser Leu Gly
245 250 255
Gly Gly Tyr Ser Tyr Ala Phe Asn Gln Ala Val Glu Asp Ala Tyr Asp
260 265 270
Glu Gly Val Leu Ser Val Val Ala Ala Gly Asn Asp Asn Ile Asp Ala
275 280 285
Ser Asp Ser Ser Pro Ala Ser Ala Pro Asn Ala Leu Thr Val Ala Ala
290 295 300
Ser Thr Lys Ser Asn Thr Arg Ala Ser Phe Ser Asn Tyr Gly Ser Val
305 310 315 320
Val Asp Ile Phe Ala Pro Gly Gln Asp Ile Leu Ser Ala Trp Ile Gly
325 330 335
Ser Thr Thr Ala Thr Asn Thr Ile Ser Gly Thr Ser Met Ala Thr Pro
340 345 350
His Val Val Gly Leu Ser Leu Tyr Leu Ile Ala Leu Glu Gly Leu Ser
355 360 365
Ser Ala Ser Ala Val Val Ser Arg Ile Lys Glu Leu Ala Thr Gln Gly
370 375 380
Val Leu Ser Asn Val Gln Gly Ser Pro Asn Leu Leu Ala Tyr Asn Gly
385 390 395 400
Ala Asp Glu
<210> 548
<211> 420
<212> PRT
<213> 嗜热毁丝霉
<400> 548
Met Ala Gly Arg Leu Leu Leu Cys Leu Thr Ala Ala Leu Ser Ala Leu
1 5 10 15
Gly Val Ser Ala Ala Pro Ala Pro Asp Ala Ser Gly Arg Pro Phe Ile
20 25 30
Gly Val Pro Val Ser Asn Pro Gly Ile Ala Asn Ala Ile Pro Asn Arg
35 40 45
Tyr Ile Val Val Tyr Asn Asn Thr Phe Asn Asp Glu Asp Ile Asp Leu
50 55 60
His Gln Ser Asn Val Ile Lys Thr Ile Ala Lys Arg Asn Ile Ala Lys
65 70 75 80
Arg Ser Leu Thr Gly Lys Leu Leu Ser Thr Thr Val Asn Thr Tyr Lys
85 90 95
Ile Asn Asn Trp Arg Ala Met Ala Leu Glu Ala Asp Asp Ala Thr Ile
100 105 110
Asn Glu Ile Phe Ala Ala Lys Glu Val Ser Tyr Ile Glu Gln Asp Ala
115 120 125
Val Ile Ser Leu Asn Val Arg Gln Met Gln Ser Gln Ala Thr Thr Gly
130 135 140
Leu Ala Arg Ile Ser His Ala Gln Pro Gly Ala Arg Thr Tyr Ile Phe
145 150 155 160
Asp Ser Ser Ala Gly Glu Gly Ile Thr Ala Tyr Val Val Asp Thr Gly
165 170 175
Ile Arg Val Thr His Glu Glu Phe Glu Gly Arg Ala Thr Phe Ala Ala
180 185 190
Asn Phe Ile Asp Asp Val Asp Thr Asp Glu Gln Gly His Gly Ser His
195 200 205
Val Ala Gly Thr Ile Gly Gly Lys Thr Phe Gly Val Ala Lys Lys Val
210 215 220
Asn Leu Val Ala Val Lys Val Leu Gly Ala Asp Gly Ser Gly Ser Asn
225 230 235 240
Ser Gly Val Ile Ala Gly Met Gln Phe Val Ala Ser Asn Ala Thr Ala
245 250 255
Met Gly Leu Lys Gly Arg Ala Val Met Asn Met Ser Leu Gly Gly Pro
260 265 270
Ala Ser Arg Ala Val Asn Ser Ala Ile Asn Gln Val Glu Ala Ala Gly
275 280 285
Val Val Pro Val Val Ala Ala Gly Asn Glu Ser Gln Asp Thr Ala Asn
290 295 300
Thr Ser Pro Gly Ser Ala Glu Ala Ala Ile Thr Val Gly Ala Ile Asp
305 310 315 320
Gln Thr Asn Asp Arg Met Ala Ser Phe Ser Asn Phe Gly Glu Leu Val
325 330 335
Asp Ile Phe Ala Pro Gly Val Asn Val Gln Ser Val Gly Ile Arg Ser
340 345 350
Asp Thr Ser Thr Asn Thr Leu Ser Gly Thr Ser Met Ala Ser Pro His
355 360 365
Val Ala Gly Leu Ala Ala Tyr Ile Met Ser Leu Glu Asn Ile Thr Gly
370 375 380
Val Gln Ala Val Ser Asp Arg Leu Lys Glu Leu Ala Gln Ala Thr Gly
385 390 395 400
Ala Arg Ala Arg Gly Val Pro Arg Gly Thr Thr Thr Leu Ile Ala Asn
405 410 415
Asn Gly Phe Ala
420
<210> 549
<211> 398
<212> PRT
<213> 产黄青霉
<400> 549
Met Gly Phe Leu Lys Val Leu Ala Thr Ser Leu Ala Thr Leu Ala Val
1 5 10 15
Val Asn Ala Gly Lys Leu Leu Thr Gly Ser Asp Ala Gly Ala Val Val
20 25 30
Pro Gly Ser Tyr Ile Val Val Met Asn Asp Gly Val Ser Asn Asp Val
35 40 45
Phe Lys Asn His Arg Asp Trp Ala Ala Thr Val His Ala Arg Ile Ala
50 55 60
Ser Arg Lys Gly Gly Glu Ser Gly Pro Gly Lys Asn Phe Asp Ile Asn
65 70 75 80
Gly Met Lys Gly Tyr Thr Ala Ser Phe Asp Asp Arg Thr Val Lys Asp
85 90 95
Ile Ala Ser Asp Pro Ala Val Lys Tyr Val Glu Pro Asp Met Val Val
100 105 110
Asn Ala Thr Glu Asn Val Val Gln Pro Asn Ala Pro Ser Trp Gly Leu
115 120 125
Pro Arg Ile Ser Ser Gln Lys Pro Gly Ala Thr Asp Tyr Val Tyr Asp
130 135 140
Ser Thr Ala Gly Glu Gly Ile Val Val Tyr Gly Val Asp Thr Gly Ile
145 150 155 160
Asp Ile Arg His Ser Asp Phe Glu Gly Arg Ala Glu Trp Gly Thr Asn
165 170 175
Thr Val Asp Asn Asp Asn Thr Asp Gly Asn Gly His Gly Thr His Thr
180 185 190
Ala Ser Thr Ala Val Gly Ser Lys Phe Gly Val Ala Lys Lys Ala Ser
195 200 205
Val Val Ala Val Lys Val Leu Ser Ala Asp Gly Ser Gly Thr Asn Ser
210 215 220
Gln Val Ile Ala Gly Met Glu Trp Ala Val Lys Asp Ala Lys Ser Arg
225 230 235 240
Gly Val Thr Gly Lys Ser Val Met Asn Met Ser Leu Gly Gly Ala Phe
245 250 255
Ser Arg Ala Met Asn Asp Ala Ala Ala Asn Val Val Lys Ser Gly Val
260 265 270
Phe Leu Ser Val Ala Ala Gly Asn Glu Ala Gln Asp Ala Ser Asn Ser
275 280 285
Ser Pro Ala Ser Ala Pro Asn Val Cys Thr Ile Ala Ala Ser Thr Ser
290 295 300
Ser Asp Arg Ser Ala Ser Phe Ser Asn Phe Gly Ser Val Val Asp Leu
305 310 315 320
Tyr Ala Pro Gly Glu Ser Ile Thr Ala Ala Tyr Pro Gly Gly Gly Ser
325 330 335
Lys Thr Leu Ser Gly Thr Ser Met Ala Ala Pro His Val Ala Gly Ala
340 345 350
Ala Ala Tyr Leu Met Ala Leu Glu Gly Val Ala Ser Asp Lys Ala Cys
355 360 365
Ala Arg Ile Val Glu Leu Ala Ile Ser Ser Ile Ser Ser Ala Pro Phe
370 375 380
Gly Thr Thr Ser Lys Leu Leu Phe Asn Gly Leu Asn Ala Lys
385 390 395
<210> 550
<211> 421
<212> PRT
<213> 粗糙链孢霉
<400> 550
Met Val Gly Leu Lys Asn Val Ala Leu Phe Ala Ala Ser Ile Ile Leu
1 5 10 15
Pro Ala Ser Ile Thr Trp Ala Ala Pro Ile Ile Glu Val Glu Thr Lys
20 25 30
Pro Ile Pro Glu Lys Tyr Ile Val Leu Leu Lys Pro His Ala Asp Leu
35 40 45
Glu Gly His Leu Ser Trp Ala Lys Asp Val His Ala Arg Ser Leu Ser
50 55 60
Arg Arg Asp Thr Ala Gly Val His Lys Ala Trp Ser Val Gly Ser Lys
65 70 75 80
Phe Lys Ala Tyr Ala Gly Glu Phe Asp Glu Glu Thr Leu Lys Ile Ile
85 90 95
Gln Arg Asp Glu Arg Asn Val His Ser Ile Glu Pro Asp Lys Ser Trp
100 105 110
Arg Leu Tyr Lys Ser Asn Lys Lys Asp Asn Asp Asp Ser Asn Ser Asp
115 120 125
Asn Thr Thr Ile Ile Thr Gln Lys Gln Ala Pro Trp Gly Leu Gly Tyr
130 135 140
Leu Ser His Lys Gly Lys Thr Ser Ser Asp Tyr Val Tyr Asn Ser Thr
145 150 155 160
Ala Gly Thr Gly Thr Tyr Ala Tyr Val Val Asp Thr Gly Cys Trp Lys
165 170 175
Asp His Val Glu Phe Glu Gly Arg Val Gln Leu Gly Tyr Asn Ala Tyr
180 185 190
Pro Asp Ser Pro Phe Ile Asp Met Asp Gly His Gly Thr His Val Thr
195 200 205
Gly Thr Leu Ile Ser Lys Thr Tyr Gly Val Ala Lys Asn Ala Thr Val
210 215 220
Ile Cys Val Lys Val Phe His Gly Gly Gly Ser Ala Asn Thr Ile Val
225 230 235 240
Met Asp Gly Phe Glu Trp Ala Val Lys Asp Ile Ile Ala Lys Lys Arg
245 250 255
Gln Arg Asn Ser Val Ile Asn Met Ser Leu Gly Cys Asp Arg Ser Glu
260 265 270
Ala Phe Asn Ala Ile Val Asp Ala Ala Tyr Asp Gln Gly Ile Leu Thr
275 280 285
Val Val Ala Ala Gly Asn Glu Asn Gln Pro Ala Ala Leu Val Ser Pro
290 295 300
Ala Ser Ser Ala Arg Ala Phe Ser Val Gly Ala Ile Asp Asn Lys Asn
305 310 315 320
Thr Arg Ala Tyr Phe Ser Asn Tyr Gly Ala Ile Val Asp Ile Phe Ala
325 330 335
Pro Gly Val Asn Ile Val Ser Thr Tyr Ile Gly Lys Lys Asp Gly Asp
340 345 350
Asn Asn Arg Thr Met Thr Met Ser Gly Thr Ser Met Ala Ser Pro His
355 360 365
Val Ala Gly Leu Ala Leu Tyr Leu Lys Ser Leu Asp Pro Glu Lys Tyr
370 375 380
Gly Asn Ser Ser Asp Ala His Ser Gly Leu Arg Ala Leu Gly Val Pro
385 390 395 400
Asp Lys Val Trp Asp Ala Gly Glu Met Ser Pro Asn Leu Val Ala Tyr
405 410 415
Asn Gly Val Gln Gly
420
<210> 551
<211> 658
<212> PRT
<213> 构巢曲霉
<400> 551
Met Ala Ala Thr Gly Arg Phe Thr Ala Phe Trp Asn Val Ala Ser Val
1 5 10 15
Pro Ala Leu Ile Gly Ile Leu Pro Leu Ala Gly Ser His Leu Arg Ala
20 25 30
Val Leu Cys Pro Val Cys Ile Trp Arg His Ser Lys Ala Val Cys Ala
35 40 45
Pro Asp Thr Leu Gln Ala Met Arg Ala Phe Thr Arg Val Thr Ala Ile
50 55 60
Ser Leu Ala Gly Phe Ser Cys Phe Ala Ala Ala Ala Ala Ala Ala Phe
65 70 75 80
Glu Ser Leu Arg Ala Val Pro Asp Gly Trp Ile Tyr Glu Ser Thr Pro
85 90 95
Asp Pro Asn Gln Pro Leu Arg Leu Arg Ile Ala Leu Lys Gln His Asn
100 105 110
Val Ala Gly Phe Glu Gln Ala Leu Leu Asp Met Ser Thr Pro Gly His
115 120 125
Ser Ser Tyr Gly Gln His Phe Gly Ser Tyr His Glu Met Lys Gln Leu
130 135 140
Leu Leu Pro Thr Glu Glu Ala Ser Ser Ser Val Arg Asp Trp Leu Ser
145 150 155 160
Ala Ala Gly Val Glu Phe Glu Gln Asp Ala Asp Trp Ile Asn Phe Arg
165 170 175
Thr Thr Val Asp Gln Ala Asn Ala Leu Leu Asp Ala Asp Phe Leu Trp
180 185 190
Tyr Thr Thr Thr Gly Ser Thr Gly Asn Pro Thr Arg Ile Leu Arg Thr
195 200 205
Leu Ser Tyr Ser Val Pro Ser Glu Leu Ala Gly Tyr Val Asn Met Ile
210 215 220
Gln Pro Thr Thr Arg Phe Gly Gly Thr His Ala Asn Arg Ala Thr Val
225 230 235 240
Arg Ala Lys Pro Ile Phe Leu Glu Thr Asn Arg Gln Leu Ile Asn Ala
245 250 255
Ile Ser Ser Gly Ser Leu Glu His Cys Glu Lys Ala Ile Thr Pro Ser
260 265 270
Cys Leu Ala Asp Leu Tyr Asn Thr Glu Gly Tyr Lys Ala Ser Asn Arg
275 280 285
Ser Gly Ser Lys Val Ala Phe Ala Ser Phe Leu Glu Glu Tyr Ala Arg
290 295 300
Tyr Asp Asp Leu Ala Glu Phe Glu Glu Thr Tyr Ala Pro Tyr Ala Ile
305 310 315 320
Gly Gln Asn Phe Ser Val Ile Ser Ile Asn Gly Gly Leu Asn Asp Gln
325 330 335
Asp Ser Thr Ala Asp Ser Gly Glu Ala Asn Leu Asp Leu Gln Tyr Ile
340 345 350
Ile Gly Val Ser Ser Pro Leu Pro Val Thr Glu Phe Thr Thr Gly Gly
355 360 365
Arg Gly Lys Leu Ile Pro Asp Leu Ser Ser Pro Asp Pro Asn Asp Asn
370 375 380
Thr Asn Glu Pro Phe Leu Asp Phe Leu Glu Ala Val Leu Lys Leu Asp
385 390 395 400
Gln Lys Asp Leu Pro Gln Val Ile Ser Thr Ser Tyr Gly Glu Asp Glu
405 410 415
Gln Thr Ile Pro Glu Pro Tyr Ala Arg Ser Val Cys Asn Leu Tyr Ala
420 425 430
Gln Leu Gly Ser Arg Gly Val Ser Val Leu Phe Ser Ser Gly Asp Ser
435 440 445
Gly Val Gly Ala Ala Cys Gln Thr Asn Asp Gly Lys Asn Thr Thr His
450 455 460
Phe Pro Pro Gln Phe Pro Ala Ser Cys Pro Trp Val Thr Ala Val Gly
465 470 475 480
Gly Thr Asn Gly Thr Ala Pro Glu Ser Gly Val Tyr Phe Ser Ser Gly
485 490 495
Gly Phe Ser Asp Tyr Trp Ala Arg Pro Ala Tyr Gln Asn Ala Ala Val
500 505 510
Glu Ser Tyr Leu Arg Lys Leu Gly Ser Thr Gln Ala Gln Tyr Phe Asn
515 520 525
Arg Ser Gly Arg Ala Phe Pro Asp Val Ala Ala Gln Ala Gln Asn Phe
530 535 540
Ala Val Val Asp Lys Gly Arg Val Gly Leu Phe Asp Gly Thr Ser Cys
545 550 555 560
Ser Ser Pro Val Phe Ala Gly Ile Val Ala Leu Leu Asn Asp Val Arg
565 570 575
Leu Lys Ala Gly Leu Pro Val Leu Gly Phe Leu Asn Pro Trp Leu Tyr
580 585 590
Gln Asp Gly Leu Asn Gly Leu Asn Asp Ile Val Asp Gly Gly Ser Thr
595 600 605
Gly Cys Asp Gly Asn Asn Arg Phe Asn Gly Ser Pro Asn Gly Ser Pro
610 615 620
Val Ile Pro Tyr Ala Gly Trp Asn Ala Thr Glu Gly Trp Asp Pro Val
625 630 635 640
Thr Gly Leu Gly Thr Pro Asp Phe Ala Lys Leu Lys Ala Leu Val Leu
645 650 655
Asp Ala
<210> 552
<211> 601
<212> PRT
<213> 产黄青霉
<400> 552
Met Ile Ala Ser Leu Phe Asn Arg Gly Ala Leu Ser Leu Ala Val Leu
1 5 10 15
Ser Leu Leu Ala Ser Ser Ala Ser Ala Asp Val Phe Glu Ser Leu Ser
20 25 30
Ala Val Pro Gln Gly Trp Arg Tyr Ser Arg Arg Pro Arg Ala Asp Gln
35 40 45
Pro Leu Lys Leu Gln Ile Ala Leu Ala Gln Gly Asp Thr Ala Gly Phe
50 55 60
Glu Glu Ala Val Met Asp Met Ser Thr Pro Asp His Pro Ser Tyr Gly
65 70 75 80
Asn His Phe His Thr His Glu Glu Met Lys Arg Met Leu Gln Pro Ser
85 90 95
Ala Glu Ser Ala Asp Ser Ile Arg Asp Trp Leu Glu Ser Ala Gly Ile
100 105 110
Asn Arg Ile Glu Gln Asp Ala Asp Trp Met Thr Phe Tyr Thr Thr Val
115 120 125
Glu Thr Ala Asn Glu Leu Leu Ala Ala Asn Phe Gln Phe Tyr Ala Asn
130 135 140
Ser Ala Lys His Ile Glu Arg Leu Arg Thr Leu Gln Tyr Ser Val Pro
145 150 155 160
Glu Ala Leu Met Pro His Ile Asn Met Ile Gln Pro Thr Thr Arg Phe
165 170 175
Gly Gln Leu Arg Val Gln Gly Ala Ile Leu His Thr Gln Val Lys Glu
180 185 190
Thr Asp Glu Ala Phe Arg Ser Asn Ala Val Ser Thr Ser Pro Asp Cys
195 200 205
Asn Ser Ile Ile Thr Pro Gln Cys Leu Lys Asn Met Tyr Asn Val Gly
210 215 220
Asp Tyr Gln Ala Asp Asp Asp Asn Gly Asn Lys Val Gly Phe Ala Ser
225 230 235 240
Tyr Leu Glu Glu Tyr Ala Arg Tyr Ser Asp Leu Glu Leu Phe Glu Lys
245 250 255
Asn Val Ala Pro Phe Ala Lys Gly Gln Asn Phe Ser Val Ile Gln Tyr
260 265 270
Asn Gly Gly Leu Asn Asp Gln His Ser Ser Ala Ser Ser Ser Glu Ala
275 280 285
Asn Leu Asp Leu Gln Tyr Ile Val Gly Val Ser Ser Pro Val Pro Val
290 295 300
Thr Glu Phe Ser Val Gly Gly Arg Gly Glu Leu Val Pro Asp Leu Asp
305 310 315 320
Gln Pro Asp Pro Asn Asp Asn Asn Asn Glu Pro Tyr Leu Glu Phe Leu
325 330 335
Gln Asn Val Leu Lys Met Glu Gln Gln Asp Leu Pro Gln Val Ile Ser
340 345 350
Thr Ser Tyr Gly Glu Asn Glu Gln Ser Val Pro Glu Lys Tyr Ala Arg
355 360 365
Thr Val Cys Asn Leu Phe Ser Gln Leu Gly Ser Arg Gly Val Ser Val
370 375 380
Ile Phe Ala Ser Gly Asp Ser Gly Val Gly Ala Ala Cys Gln Thr Asn
385 390 395 400
Asp Gly Arg Asn Ala Thr Arg Phe Pro Ala Gln Phe Pro Ala Ala Cys
405 410 415
Pro Trp Val Thr Ser Val Gly Ala Thr Thr His Thr Ala Pro Glu Lys
420 425 430
Ala Val Tyr Phe Ser Ser Gly Gly Phe Ser Asp Leu Trp Asp Arg Pro
435 440 445
Lys Trp Gln Glu Asp Ala Val Ser Asp Tyr Leu Asp Thr Leu Gly Asp
450 455 460
Arg Trp Ser Gly Leu Phe Asn Pro Lys Gly Arg Ala Phe Pro Asp Val
465 470 475 480
Ser Ala Gln Gly Gln Asn Tyr Ala Ile Tyr Asp Lys Gly Ser Leu Thr
485 490 495
Ser Val Asp Gly Thr Ser Cys Ser Ala Pro Ala Phe Ala Gly Val Ile
500 505 510
Ala Leu Leu Asn Asp Ala Arg Leu Lys Ala Asn Lys Pro Pro Met Gly
515 520 525
Phe Leu Asn Pro Trp Leu Tyr Ser Thr Gly Arg Asp Gly Leu Asn Asp
530 535 540
Ile Val His Gly Gly Ser Thr Gly Cys Asp Gly Asn Ala Arg Phe Gly
545 550 555 560
Gly Pro Gly Asn Gly Ser Pro Arg Val Pro Gly Ala Ser Trp Asn Ala
565 570 575
Thr Lys Gly Trp Asp Pro Val Ser Gly Leu Gly Ser Pro Asn Phe Ala
580 585 590
Thr Met Arg Lys Leu Ala Asn Gly Glu
595 600
<210> 553
<211> 588
<212> PRT
<213> 黑曲霉
<400> 553
Met Ile Tyr Val Asn Tyr Ile Leu Gly Leu Leu Ser Leu Leu His Thr
1 5 10 15
Ala Val Ala Thr Ala Pro Asp Tyr Val Val Val Asp Gln Leu Asn Ser
20 25 30
Ile Pro Asp Gly Trp Thr Lys Gly Ala Ala Pro Pro Pro Phe Thr Pro
35 40 45
Met Lys Phe Trp Leu Ser Met His His Glu Tyr Lys Ala Asp Phe Glu
50 55 60
Gln Lys Val Ile Asp Ile Ser Thr Pro Gly His Arg Asp Tyr Gly Arg
65 70 75 80
His Met Lys Arg Asn Asp Val Met Ala Phe Met Arg Pro Ser Asp Gln
85 90 95
Val Ser Lys Ile Ile Phe Ser Trp Leu Glu Ser Glu His Val Pro Pro
100 105 110
Asn Ala Ile Glu Asp Arg Gly Asp Trp Val Ala Phe Thr Val Pro Leu
115 120 125
Ala Gln Ala Gln Ser Met Met Lys Thr Asp Phe Tyr Asn Phe His His
130 135 140
Leu Glu Thr Asn Thr Thr Gln Ile Arg Thr Leu Lys Tyr Ser Val Pro
145 150 155 160
Glu Gln Val Asp Ala His Leu Gln Met Ile Gln Pro Thr Thr Arg Phe
165 170 175
Gly Arg Pro Lys Thr Gln Thr Ser Leu Pro Ser Leu Met Pro Val Ser
180 185 190
Val Asn Ile Asp Glu Ile Ser Glu Asp Cys Leu Thr Gly Val Thr Pro
195 200 205
Ile Cys Leu Arg Gln Leu Tyr Gly Leu Pro Ser Thr Lys Ala Ser Pro
210 215 220
Asp Ser Arg Asn Val Leu Gly Ile Ser Gly Tyr Leu Asp Gln Tyr Ala
225 230 235 240
Arg Tyr Ser Asp Leu Asp Glu Phe Leu Ala Val Tyr Ser Pro Asn Ser
245 250 255
Val Asp Ala Asp Phe Ser Val Val Ser Ile Asn Gly Gly Gln Asn Pro
260 265 270
Gln Asn Ser Gln Glu Gly Ser Thr Glu Ala Ser Leu Asp Ile Gln Tyr
275 280 285
Ala Leu Ser Met Ala Phe Asp Ala Asn Ala Thr Phe Tyr Thr Thr Ala
290 295 300
Gly Arg Ala Pro Ser Val Ser Asp Ser Gly Thr Val Ser Thr Asp Gly
305 310 315 320
Ser Thr Asn Glu Pro Tyr Leu Glu Gln Leu Gln Tyr Leu Val Gly Leu
325 330 335
Pro Asp Glu Asp Leu Pro Ala Val Leu Ser Thr Ser Tyr Gly Glu Asp
340 345 350
Glu Gln Ser Leu Pro Glu Glu Tyr Thr Glu Ala Thr Cys Asn Leu Phe
355 360 365
Ala Gln Leu Gly Ala Arg Gly Val Ser Val Ile Phe Ser Ser Gly Asp
370 375 380
Ser Gly Val Gly Gly Ser Cys Val Ser Asn Asp Gly Ser Gln Arg Thr
385 390 395 400
Arg Phe Gln Pro Ile Phe Pro Ala Ser Cys Pro Phe Val Thr Ser Val
405 410 415
Gly Gly Thr Glu Gly Val Gly Pro Glu Lys Ala Val Asp Phe Ser Ser
420 425 430
Gly Gly Phe Ser Glu Arg Phe Ala Arg Pro Ser Tyr Gln Asn Ala Ser
435 440 445
Val Glu Ala Tyr Leu Ala Arg Leu Gly Asp Lys Trp Asp Gly Leu Tyr
450 455 460
Asn Pro Asp Gly Arg Gly Ile Pro Asp Val Ser Ala Gln Ala Ser Asn
465 470 475 480
Tyr Val Ile Arg Asp His Gly Gln Trp Leu Gln Thr Ala Gly Thr Ser
485 490 495
Ala Ala Ala Pro Val Phe Ala Ala Val Ile Ser Arg Leu Asn Ala Ala
500 505 510
Arg Leu Glu Gln Gly Lys Pro Thr Leu Gly Phe Leu Asn Pro Trp Leu
515 520 525
Tyr Ser Leu Asp Gln Gln Gly Phe Thr Asp Ile Val Asp Gly Gly Ser
530 535 540
Val Gly Cys Asp Gly Ser Asn Gly Gly Ala Leu Val Pro Tyr Ala Ser
545 550 555 560
Trp Asn Ala Thr Lys Gly Trp Asp Pro Val Thr Gly Leu Gly Thr Pro
565 570 575
Leu Tyr Gln Thr Leu Glu Gln Leu Ala Gln Ser Ala
580 585
<210> 554
<211> 600
<212> PRT
<213> 米曲霉
<400> 554
Met Phe Phe Ser Arg Gly Ala Leu Ser Leu Ala Val Leu Ser Leu Leu
1 5 10 15
Ser Ser Ser Ala Ala Gly Glu Ala Phe Glu Lys Leu Ser Ala Val Pro
20 25 30
Lys Gly Trp His Tyr Ser Ser Thr Pro Lys Gly Asn Thr Glu Val Cys
35 40 45
Leu Lys Ile Ala Leu Ala Gln Lys Asp Ala Ala Gly Phe Glu Lys Thr
50 55 60
Val Leu Glu Met Ser Asp Pro Asp His Pro Ser Tyr Gly Gln His Phe
65 70 75 80
Thr Thr His Asp Glu Met Lys Arg Met Leu Leu Pro Arg Asp Asp Thr
85 90 95
Val Asp Ala Val Arg Gln Trp Leu Glu Asn Gly Gly Val Thr Asp Phe
100 105 110
Thr Gln Asp Ala Asp Trp Ile Asn Phe Cys Thr Thr Val Asp Thr Ala
115 120 125
Asn Lys Leu Leu Asn Ala Gln Phe Lys Trp Tyr Val Ser Asp Val Lys
130 135 140
His Ile Arg Arg Leu Arg Thr Leu Gln Tyr Asp Val Pro Glu Ser Val
145 150 155 160
Thr Pro His Ile Asn Thr Ile Gln Pro Thr Thr Arg Phe Gly Lys Ile
165 170 175
Ser Pro Lys Lys Ala Val Thr His Ser Lys Pro Ser Gln Leu Asp Val
180 185 190
Thr Ala Leu Ala Ala Ala Val Val Ala Lys Asn Ile Ser His Cys Asp
195 200 205
Ser Ile Ile Thr Pro Thr Cys Leu Lys Glu Leu Tyr Asn Ile Gly Asp
210 215 220
Tyr Gln Ala Asp Ala Asn Ser Gly Ser Lys Ile Ala Phe Ala Ser Tyr
225 230 235 240
Leu Glu Glu Tyr Ala Arg Tyr Ala Asp Leu Glu Asn Phe Glu Asn Tyr
245 250 255
Leu Ala Pro Trp Ala Lys Gly Gln Asn Phe Ser Val Thr Thr Phe Asn
260 265 270
Gly Gly Leu Asn Asp Gln Asn Ser Ser Ser Asp Ser Gly Glu Ala Asn
275 280 285
Leu Asp Leu Gln Tyr Ile Leu Gly Val Ser Ala Pro Leu Pro Val Thr
290 295 300
Glu Phe Ser Thr Gly Gly Arg Gly Pro Leu Val Pro Asp Leu Thr Gln
305 310 315 320
Pro Asp Pro Asn Ser Asn Ser Asn Glu Pro Tyr Leu Glu Phe Phe Gln
325 330 335
Asn Val Leu Lys Leu Asp Gln Lys Asp Leu Pro Gln Val Ile Ser Thr
340 345 350
Ser Tyr Gly Glu Asn Glu Gln Glu Ile Pro Glu Lys Tyr Ala Arg Thr
355 360 365
Val Cys Asn Leu Ile Ala Gln Leu Gly Ser Arg Gly Val Ser Val Leu
370 375 380
Phe Ser Ser Gly Asp Ser Gly Val Gly Glu Gly Cys Met Thr Asn Asp
385 390 395 400
Gly Thr Asn Arg Thr His Phe Pro Pro Gln Phe Pro Ala Ala Cys Pro
405 410 415
Trp Val Thr Ser Val Gly Ala Thr Phe Lys Thr Thr Pro Glu Arg Gly
420 425 430
Thr Tyr Phe Ser Ser Gly Gly Phe Ser Asp Tyr Trp Pro Arg Pro Glu
435 440 445
Trp Gln Asp Glu Ala Val Ser Ser Tyr Leu Glu Thr Ile Gly Asp Thr
450 455 460
Phe Lys Gly Leu Tyr Asn Ser Ser Gly Arg Ala Phe Pro Asp Val Ala
465 470 475 480
Ala Gln Gly Met Asn Phe Ala Val Tyr Asp Lys Gly Thr Leu Gly Glu
485 490 495
Phe Asp Gly Thr Ser Ala Ser Ala Pro Ala Phe Ser Ala Val Ile Ala
500 505 510
Leu Leu Asn Asp Ala Arg Leu Arg Ala Gly Lys Pro Thr Leu Gly Phe
515 520 525
Leu Asn Pro Trp Leu Tyr Lys Thr Gly Arg Gln Gly Leu Gln Asp Ile
530 535 540
Thr Leu Gly Ala Ser Ile Gly Cys Thr Gly Arg Ala Arg Phe Gly Gly
545 550 555 560
Ala Pro Asp Gly Gly Pro Val Val Pro Tyr Ala Ser Trp Asn Ala Thr
565 570 575
Gln Gly Trp Asp Pro Val Thr Gly Leu Gly Thr Pro Asp Phe Ala Glu
580 585 590
Leu Lys Lys Leu Ala Leu Gly Asn
595 600
<210> 555
<211> 587
<212> PRT
<213> 粗糙链孢霉
<400> 555
Met Leu Trp Ser Val Leu Leu Leu Ala Ala Gly Ala Ser Ala His Val
1 5 10 15
Lys Ser Ser Leu Pro Ser Val Pro Ser Gly Trp Lys Lys Val Arg Ala
20 25 30
Ala Ser Ala Asp Glu Ser Val Ser Leu Lys Ile Ala Leu Pro Ala His
35 40 45
Gln Pro Asp Ala Leu Glu Thr Ala Ile Leu Arg Val Ser Asp Pro Asn
50 55 60
His His Glu Tyr Gly Met His Leu Ser Ser Glu Glu Val Arg Ser Leu
65 70 75 80
Val Ala Pro Ala Asp Glu Thr Thr Asp Ala Val Thr Ser Trp Leu Asn
85 90 95
Arg Asn Gly Ile Lys Gly Lys Val Asp Asn Asp Trp Val Ser Phe Thr
100 105 110
Thr Ser Val Ala Lys Ala Asn Asn Leu Leu Asn Thr Thr Phe Asp Trp
115 120 125
Tyr Gln Gln Asp Gly Asp Lys Thr Gly Pro Lys Leu Arg Thr Leu Gln
130 135 140
Tyr Ser Val Pro Asp Glu Leu Asp Ala His Val Asp Met Ile Gln Pro
145 150 155 160
Thr Thr Arg Phe Gly Lys Leu Ala Ala Lys Ala Ser Thr Ile Phe Glu
165 170 175
Ile Phe Asp Glu Pro Glu Pro Lys Asn Ile Ala Asn Val Lys Val Gly
180 185 190
Gly Asp His Pro Thr Cys Thr Gly Cys Ile Tyr Pro Asp Glu Ile Arg
195 200 205
Ser Leu Tyr Asn Ile Lys Tyr Lys Pro Ser Ala Ser Asp Lys Asn Thr
210 215 220
Ile Ala Phe Ala Ser Tyr Leu Glu Gln Tyr Ser Asn Tyr Asp Asp Phe
225 230 235 240
Thr Ser Phe Ala Lys Ala Phe Ile Pro Asp Ala Ala Asp Arg Asn Tyr
245 250 255
Thr Val Lys Leu Val Lys Gly Gly Leu His Asp Gln Ser Pro Asp Lys
260 265 270
Ile Gly Val Glu Ala Asn Leu Asp Leu Gln Tyr Ile Leu Ala Ile Ser
275 280 285
Asn Pro Ile Pro Ile Arg Glu Tyr Ser Ile Gly Gly Arg Gly Pro Leu
290 295 300
Val Pro Thr Ala Asn Gln Pro Gly Pro Glu Ile Ser Asn Glu Pro Tyr
305 310 315 320
Leu Asp Phe Phe Gln Tyr Leu Leu Ser Leu Lys Asn Ser Glu Leu Pro
325 330 335
Ala Thr Leu Ser Thr Ser Tyr Gly Glu Glu Glu Gln Ser Val Pro Arg
340 345 350
Glu Tyr Ala Leu Lys Val Cys Ser Met Ile Gly Gln Leu Gly Ala Arg
355 360 365
Gly Val Ser Val Ile Phe Ser Ser Gly Asp Ser Gly Pro Gly Asp Ala
370 375 380
Cys Ile Arg Asn Asp Gly Thr Asn Ser Thr Tyr Phe Glu Pro Thr Phe
385 390 395 400
Pro Gly Ala Cys Pro Trp Val Thr Ser Val Gly Gly Thr Tyr Gln Thr
405 410 415
Gly Pro Glu Lys Ala Val Asp Phe Ser Ser Gly Gly Phe Ser Met Tyr
420 425 430
His Lys Arg Pro Val Tyr Gln Glu Arg Val Val Lys Lys Tyr Leu Asp
435 440 445
Lys Ile Gly Asp Thr Tyr Ser Asp Phe Phe Asp Glu Gln Gly Arg Gly
450 455 460
Phe Pro Asp Val Ser Ala Gln Ala Ser Arg Tyr Ala Val Tyr Val Asp
465 470 475 480
Gly Arg Leu Val Gly Val Ser Gly Thr Ser Ala Ser Ala Pro Met Phe
485 490 495
Ala Gly Leu Val Ala Leu Leu Asn Ala Ala Arg Lys Ser His Gly Leu
500 505 510
Pro Ser Leu Gly Phe Ile Asn Pro Leu Leu Tyr Ala Ser Lys Asp Ala
515 520 525
Phe Thr Asp Ile Val Asn Gly Ala Gly Thr Gly Cys Arg Gly Arg Pro
530 535 540
Glu Phe Ala Gly Asp Val Gly Gly Thr Ala Lys Trp Asn Ala Thr Glu
545 550 555 560
Gly Trp Asp Pro Val Thr Gly Leu Gly Thr Pro Lys Phe Asp Lys Leu
565 570 575
Leu Ala Leu Ala Ala Pro Gly Val Lys Asn Ala
580 585
<210> 556
<211> 644
<212> PRT
<213> 烟曲霉
<400> 556
Met Arg Leu Ser His Val Leu Leu Gly Thr Ala Ala Ala Ala Gly Val
1 5 10 15
Leu Ala Ser Pro Thr Pro Asn Asp Tyr Val Val His Glu Arg Arg Ala
20 25 30
Val Leu Pro Arg Ser Trp Thr Glu Glu Lys Arg Leu Asp Lys Ala Ser
35 40 45
Ile Leu Pro Met Arg Ile Gly Leu Thr Gln Ser Asn Leu Asp Arg Gly
50 55 60
His Asp Leu Leu Met Glu Ile Ser Asp Pro Arg Ser Ser Arg Tyr Gly
65 70 75 80
Gln His Leu Ser Val Glu Glu Val His Ser Leu Phe Ala Pro Ser Gln
85 90 95
Glu Thr Val Asp Arg Val Arg Ala Trp Leu Glu Ser Glu Gly Ile Ala
100 105 110
Gly Asp Arg Ile Ser Gln Ser Ser Asn Glu Gln Phe Leu Gln Phe Asp
115 120 125
Ala Ser Ala Ala Glu Val Glu Arg Leu Leu Gly Thr Glu Tyr Tyr Leu
130 135 140
Tyr Thr His Gln Gly Ser Gly Lys Ser His Ile Ala Cys Arg Glu Tyr
145 150 155 160
His Val Pro His Ser Leu Gln Arg His Ile Asp Tyr Ile Thr Pro Gly
165 170 175
Ile Lys Leu Leu Glu Val Glu Gly Val Lys Lys Ala Arg Ser Ile Glu
180 185 190
Lys Arg Ser Phe Arg Ser Pro Leu Pro Pro Ile Leu Glu Arg Leu Thr
195 200 205
Leu Pro Leu Ser Glu Leu Leu Gly Asn Thr Leu Leu Cys Asp Val Ala
210 215 220
Ile Thr Pro Leu Cys Ile Ser Ala Leu Tyr Asn Ile Thr Arg Gly Ser
225 230 235 240
Lys Ala Thr Lys Gly Asn Glu Leu Gly Ile Phe Glu Asp Leu Gly Asp
245 250 255
Val Tyr Ser Gln Glu Asp Leu Asn Leu Phe Phe Ser Thr Phe Ala Gln
260 265 270
Gln Ile Pro Gln Gly Thr His Pro Ile Leu Lys Ala Val Asp Gly Ala
275 280 285
Gln Ala Pro Thr Ser Val Thr Asn Ala Gly Pro Glu Ser Asp Leu Asp
290 295 300
Phe Gln Ile Ser Tyr Pro Ile Ile Trp Pro Gln Asn Ser Ile Leu Phe
305 310 315 320
Gln Thr Asp Asp Pro Asn Tyr Thr Ala Asn Tyr Asn Phe Ser Gly Phe
325 330 335
Leu Asn Thr Phe Leu Asp Ala Ile Asp Gly Ser Tyr Cys Ser Glu Ile
340 345 350
Ser Pro Leu Asp Pro Pro Tyr Pro Asn Pro Ala Asp Gly Gly Tyr Lys
355 360 365
Gly Gln Leu Gln Cys Gly Val Tyr Gln Pro Pro Lys Val Leu Ser Ile
370 375 380
Ser Tyr Gly Gly Ala Glu Ala Asp Leu Pro Ile Ala Tyr Gln Arg Arg
385 390 395 400
Gln Cys Ala Glu Trp Met Lys Leu Gly Leu Gln Gly Val Ser Val Val
405 410 415
Val Ala Ser Gly Asp Ser Gly Val Glu Gly Arg Asn Gly Asp Pro Thr
420 425 430
Pro Thr Glu Cys Leu Gly Thr Glu Gly Lys Val Phe Ala Pro Asp Phe
435 440 445
Pro Ala Thr Cys Pro Tyr Leu Thr Thr Val Gly Gly Thr Tyr Leu Pro
450 455 460
Leu Gly Ala Asp Pro Arg Lys Asp Glu Glu Val Ala Val Thr Ser Phe
465 470 475 480
Pro Ser Gly Gly Gly Phe Ser Asn Ile Tyr Glu Arg Ala Asp Tyr Gln
485 490 495
Gln Gln Ala Val Glu Asp Tyr Phe Ser Arg Ala Asp Pro Gly Tyr Pro
500 505 510
Phe Tyr Glu Ser Val Asp Asn Ser Ser Phe Ala Glu Asn Gly Gly Ile
515 520 525
Tyr Asn Arg Ile Gly Arg Ala Tyr Pro Asp Val Ala Ala Ile Ala Asp
530 535 540
Asn Val Val Ile Phe Asn Lys Gly Met Pro Thr Leu Ile Gly Gly Thr
545 550 555 560
Ser Ala Ala Ala Pro Val Phe Ala Ala Ile Leu Thr Arg Ile Asn Glu
565 570 575
Glu Arg Leu Ala Val Gly Lys Ser Thr Val Gly Phe Val Asn Pro Val
580 585 590
Leu Tyr Ala His Pro Glu Val Phe Asn Asp Ile Thr Gln Gly Ser Asn
595 600 605
Pro Gly Cys Gly Met Gln Gly Phe Ser Ala Ala Thr Gly Trp Asp Pro
610 615 620
Val Thr Gly Leu Gly Thr Pro Asn Tyr Pro Ala Leu Leu Asp Leu Phe
625 630 635 640
Met Ser Leu Pro
<210> 557
<211> 645
<212> PRT
<213> 米曲霉
<400> 557
Met Lys Thr Ser Phe Leu Leu Leu His Thr Leu Val Ala Gly Val Leu
1 5 10 15
Ala Ile Pro Thr Arg Thr Asp Tyr Val Leu His Glu Arg Arg Asp Ala
20 25 30
Val Pro Ala His Trp Thr Gly Glu Lys Arg Leu Asp Gly Gln Thr Val
35 40 45
Leu Pro Met Arg Ile Gly Leu Thr Gln Ser Asn Leu Asp Arg Gly His
50 55 60
Asp Leu Leu Met Glu Val Ser Thr Pro Gly Ser Pro Arg Tyr Gly Asp
65 70 75 80
His Met Thr Leu Asp Glu Val His Asn Leu Phe Ala Pro Ser Gln Asp
85 90 95
Ser Val Asp Ser Val Arg Ser Trp Leu Glu Ser Ala Gly Ile Ser Pro
100 105 110
Asp Arg Ile Ser Gln Ser Thr Asn Lys Gln Trp Leu Gln Phe Asp Ala
115 120 125
Gly Val Asp Glu Val Glu Gln Leu Leu Lys Thr Glu Tyr Tyr Arg Tyr
130 135 140
Ser His Ala Gly Thr Gly Arg Ser His Val Ala Cys Arg Glu Tyr His
145 150 155 160
Val Pro Glu Ser Val Gln Ser His Ile Asp Tyr Ile Thr Pro Gly Ile
165 170 175
Lys His Leu Glu Ile Arg Glu Glu Lys Pro Val Glu Lys Arg Ser Leu
180 185 190
Asp Lys Arg Ser Phe Gly Ile Leu Pro Pro Ile Leu Arg Pro Leu Thr
195 200 205
Leu Pro Leu Glu Glu Leu Leu Gly Gln Leu Leu Leu Leu Cys Asp Val
210 215 220
Ala Val Thr Pro Ala Cys Ile Gln Ala Met Tyr Asn Val Thr Asp Gly
225 230 235 240
Asp Lys Ala Thr Lys Gly Asn Glu Leu Gly Ile Phe Glu Asp Leu Gly
245 250 255
Asp Val Tyr Ser Gln Asp Asp Leu Asp Leu Phe Phe Ser Thr Val Ala
260 265 270
His Lys Ile Pro Thr Gly Thr His Pro Ile Leu Asn Ala Ile Asp Gly
275 280 285
Ala Gln Ala Pro Ala Asp Thr Thr Asn Ala Gly Thr Glu Ser Asp Leu
290 295 300
Asp Phe Glu Ile Ser Tyr Pro Leu Ile Trp Pro Gln Asn Ser Ile Leu
305 310 315 320
Phe Gln Thr Asp Asp Pro Ile Tyr Gln Asn Asn Tyr Thr Tyr Asn Gly
325 330 335
Phe Leu Asn Asn Phe Leu Asp Ala Ile Asp Gly Ser Tyr Cys Ser Glu
340 345 350
Ala Ser Pro Leu Asp Pro Pro Tyr Pro Asn Pro Ala Asp Gly Gly Tyr
355 360 365
Lys Ser Pro Arg Gln Cys Gly Val Tyr Lys Pro Thr Asn Val Ile Ser
370 375 380
Ile Ser Tyr Gly Gly Ala Glu Ala Asp Leu Pro Ile Ala Tyr Gln Arg
385 390 395 400
Arg Gln Cys Gln Glu Phe Met Lys Leu Gly Leu Gln Gly Val Ser Ile
405 410 415
Val Val Ala Ser Gly Asp Ser Gly Val Gln Gly Arg Gly Gly Ser Pro
420 425 430
Thr Pro Ser Gly Cys Leu Gly Lys Asp Asn Lys Val Phe Ala Pro Asp
435 440 445
Phe Pro Ala Thr Cys Pro Tyr Leu Thr Thr Ala Gly Gly Thr Tyr Leu
450 455 460
Pro Pro Gly Ala Asp Val His Ala His Glu Glu Gln Ala Thr Thr Ser
465 470 475 480
Phe Pro Ser Gly Gly Gly Phe Ser Asn Ile Tyr Gln Arg Pro Asp Tyr
485 490 495
Gln Asn Ala Ala Val Glu Glu Tyr Phe Asn Thr Ala Gln Leu Ser Tyr
500 505 510
Pro Tyr Tyr Glu Ser Val Asp Asn Ser Ser Phe Ala Ala Asn Gly Gly
515 520 525
Ile Tyr Asn Arg Ile Gly Arg Ala Tyr Pro Asp Val Ala Ala Ile Ala
530 535 540
Asp Asn Val Leu Val Phe Asn Lys Gly Leu Pro Thr Leu Val Gly Gly
545 550 555 560
Thr Ser Ala Ala Ala Pro Val Phe Ala Ala Leu Leu Thr Arg Ile Asn
565 570 575
Glu Glu Arg Leu Ala Ala Gly Lys Lys Thr Val Gly Phe Val Asn Pro
580 585 590
Val Leu Tyr Ala Asn Pro Gly Val Phe Phe Asp Val Thr Lys Gly Ser
595 600 605
Asn Gln Gly Cys Gly Thr Asp Gly Phe Pro Ala Val Lys Gly Trp Asp
610 615 620
Pro Val Thr Gly Leu Gly Thr Pro Asn Tyr Pro Lys Leu Leu Glu Leu
625 630 635 640
Phe Met Gly Leu Asp
645
<210> 558
<211> 642
<212> PRT
<213> 产黄青霉
<400> 558
Met Leu Cys Val Leu Ala Ala Ser Ala Met Ala Val Pro Thr Ala His
1 5 10 15
Asp Tyr Gln Leu His Glu Arg Arg Asp Phe Ile Pro Lys Ser Trp Val
20 25 30
Glu Gly Lys Glu Leu Asp Gly Asn Val Ser Leu Pro Val Arg Ile Gly
35 40 45
Leu Thr Gln Ser Asn Leu Asp Tyr Gly His Glu Leu Leu Met Asp Leu
50 55 60
Ser Asn Pro His Ser Ser Arg Tyr Gly Gln Tyr Leu Ser Val Asn Glu
65 70 75 80
Val His Asp Leu Phe Ala Pro Thr Glu Lys Ser Val Asn Asp Val Arg
85 90 95
Tyr Trp Leu Glu Ser Ala Gly Ile Ala Lys Glu Arg Ile Thr Gln Ser
100 105 110
Ala Asn Lys Gln Trp Ile Gln Phe Asp Ala Asp Ala Glu Glu Leu Glu
115 120 125
His Leu Leu His Thr Lys Tyr Tyr Leu Tyr Ser His Ala Glu Thr Gly
130 135 140
Arg Ser His Val Ala Cys Arg Glu Tyr Gly Phe His Leu Asp Trp Ile
145 150 155 160
Tyr Ser Leu Ala Asp His Arg Pro Gln Ile Ser Cys Pro Glu Leu Arg
165 170 175
Ile Ser Leu Arg Glu Val Thr Ser Val Arg Arg Ser Thr Lys Gln Lys
180 185 190
Arg Phe Met Asp Gly Val Pro Pro Ile Leu Glu Pro Ile Leu Leu Pro
195 200 205
Ile Glu Glu Leu Leu Ser Gln Ser Pro Ser Leu Cys Ser Gln Ala Ile
210 215 220
Thr Pro Gln Cys Ile Gln Gln Met Tyr Asn Ile Ser Glu Gly His Ser
225 230 235 240
Ala Thr Glu Gly Asn Glu Leu Gly Ile Phe Glu Gly Ile Gly Asp Val
245 250 255
Tyr Ala Gln Glu Asp Leu Asp Leu Phe Phe Ser Lys Leu Tyr Ser Lys
260 265 270
Ile Pro Gln Gly Thr His Pro Ile Leu Lys Ser Val Asp Gly Gly Glu
275 280 285
Ala Pro Thr Asp Thr Ser Arg Ala Gly Pro Glu Ser Asp Leu Asp Phe
290 295 300
Gln Ile Ser Tyr Pro Ile Ile Trp Pro Gln Asn Ser Ile Leu Phe Gln
305 310 315 320
Ser Asp Asp Met His Tyr Glu Asn His Tyr Thr Phe Arg Gly Phe Leu
325 330 335
Asn Thr Phe Leu Asp Ala Ile Asp Gly Ser Tyr Cys Ser Thr Ile Ser
340 345 350
Pro Leu Asp Pro Pro Tyr Pro Asp Pro Ala Asp Gly Gly Tyr Lys Gly
355 360 365
Ser Leu Gln Cys Gly Val Tyr Asp Pro Pro Lys Val Ile Ser Ile Ser
370 375 380
Tyr Gly Ser Ala Glu Ala Asp Leu Pro Ile Ser Tyr Gln Arg Arg Gln
385 390 395 400
Cys Ala Glu Phe Met Lys Leu Gly Thr Met Gly Val Ser Val Leu Val
405 410 415
Ala Ser Gly Asp Ser Gly Val Ser Gly Arg Gly Gly Asp Pro Thr Pro
420 425 430
Ser Asn Cys Leu Gly Thr Asn Gly Arg Val Phe Ala Pro Asp Phe Pro
435 440 445
Ala Thr Cys Pro Tyr Leu Thr Ala Val Gly Gly Thr Glu Ile Pro Thr
450 455 460
Gly Ser Ser Pro Glu Asp His Gln Glu Gln Ala Val Thr Arg Phe Pro
465 470 475 480
Ser Gly Gly Gly Phe Ser Asn Ile Tyr Lys Ala Pro Glu Tyr Gln Ala
485 490 495
Gln Ala Val Ala Asp Tyr Phe Asp Lys Ala Gln Pro Ser Tyr Pro Tyr
500 505 510
Tyr Glu Ser Val Asp Asn Ser Ser Phe Gly Glu Asn Ser Gly Ile Tyr
515 520 525
Asn Arg Ile Gly Arg Ala Tyr Pro Asp Val Ala Ala Val Gly Asp Lys
530 535 540
Val Val Ile Tyr Asn Arg Gly Met Ala Val Ser Ile Gly Gly Thr Ser
545 550 555 560
Ala Ser Ala Pro Val Phe Ala Ala Ile Leu Thr Arg Ile Asn Glu Glu
565 570 575
Arg Leu Ala Ala Gly Lys Ser Thr Val Gly Phe Val Asn Pro Val Leu
580 585 590
Tyr Ala His Pro Glu Ala Phe Phe Asp Val Val Thr Gly Ser Asn Pro
595 600 605
Gly Cys Asn Thr Asp Gly Phe Ser Ala Ala Lys Gly Trp Asp Pro Val
610 615 620
Thr Gly Leu Gly Thr Pro Asn Tyr Pro Glu Leu Leu Arg Val Phe Met
625 630 635 640
Gly Glu
<210> 559
<211> 611
<212> PRT
<213> 构巢曲霉
<400> 559
Met Trp Leu Ser Leu Leu Tyr Leu Val Ser Ser Ile Thr Ala Val Leu
1 5 10 15
Ala Phe Ser Pro Pro Ala Lys His Pro Leu His Glu Arg Arg Asp Ala
20 25 30
Leu Pro Asp Tyr Trp Val Glu Asp Ala Arg Leu Asp Gly Gln Thr Val
35 40 45
Leu Pro Val Arg Ile Gly Met Thr Gln Ser Asn Leu Asp His Gly His
50 55 60
Asp Leu Leu Met Leu Ile Ser Asp Pro Ser Ser Pro Glu Tyr Gly Lys
65 70 75 80
Tyr Leu Ser Ala Lys Glu Val His Asp Leu Phe Ala Pro Ala Glu Glu
85 90 95
Thr Val Glu Arg Val Arg Ala Trp Leu Glu Ser Glu Gly Ile Ser Asp
100 105 110
Thr Arg Ile Ser Gln Ser Ala Asn Lys Gln Trp Leu Arg Phe Asp Ala
115 120 125
Thr Ala Gln Glu Leu Glu Arg Leu Leu Gln Thr Glu Tyr Tyr Leu Tyr
130 135 140
Ser His Ala Glu Thr Arg Arg Ser His Ile Ala Cys Arg Glu Tyr His
145 150 155 160
Val Pro Glu Ala Leu Gln Lys His Ile Asp Tyr Ile Thr Pro Gly Val
165 170 175
Lys Phe Leu Glu Ile Ala Gly Pro Leu Pro Ile Leu Pro Thr Glu Arg
180 185 190
Ser Leu Thr Arg Arg Asp Thr Ala Asp Ala Ser Ser Pro Ile Ile Asp
195 200 205
Gly Thr Asn Leu Thr Leu Ser Gln Val Thr Asn Asp Ala Leu Gly Phe
210 215 220
Cys Asp Gln Ile Ile Thr Pro Ala Cys Ile Arg Gly Met Pro Cys Ile
225 230 235 240
Cys Gln Pro Ile Val His Ser Ile Asn Gly Gly Thr Ala Pro Thr Thr
245 250 255
Leu Asp Asn Ala Gly Pro Glu Ser Thr Leu Asp Leu Ala Met Ser Tyr
260 265 270
Pro Ile Ile Trp Pro Gln Asn Pro Ile Ile Phe Gln Thr Asp Asp Pro
275 280 285
Ile Trp Ala Asp Ile Asn Asn Pro Lys Phe Pro Gly Ile Phe Asn Asn
290 295 300
Phe Leu Asp Ala Ile Asp Gly Ser Tyr Cys Asp Pro Ser Glu Glu Glu
305 310 315 320
Leu Asp Pro Pro Tyr Pro Asn Pro Ala Pro Gly Gly Tyr Lys Gly Gln
325 330 335
Lys Gln Cys Gly Val Tyr Lys Pro Thr Asn Val Ile Ser Ile Ser Tyr
340 345 350
Gly Phe Ala Glu Phe Glu Leu Pro Ile Arg Tyr Asn Arg Arg Gln Cys
355 360 365
Tyr Glu Trp Met Lys Leu Gly Leu Gln Gly Val Ser Val Val Ala Ala
370 375 380
Ser Gly Asn Asn Gly Val Ala Thr Gly Gly Cys Leu Gly Lys Asp Arg
385 390 395 400
Ser Val Phe Thr Pro Gly Tyr Leu Ile Cys Pro Tyr Ile Thr Thr Val
405 410 415
Gly Gly Thr Tyr Leu Leu Pro Gly Gly Asp Pro Tyr Asn Pro Leu Glu
420 425 430
Val Ala Val Asp Pro Ser Thr Thr Pro Gly Asn Trp Ser Ser Gly Gly
435 440 445
Gly Phe Ser Asn Ile Tyr Glu Arg Pro Met Tyr Gln Ala Ser Ala Val
450 455 460
Glu Glu Tyr Phe Ser Arg Ala Asn Leu Asn Tyr Pro Tyr Tyr Glu Ser
465 470 475 480
Ile Asn Asn Asp Ser Phe Ala Ala Asn Asp Gly Ile Tyr Asn Arg Ile
485 490 495
Gly Arg Ala Tyr Pro Asp Ile Ser Ala Val Ala Val Asn Asn Phe Val
500 505 510
Phe Trp Arg Gly Gly Pro Ile Leu Gly Gly Gly Thr Ser Ala Ser Ala
515 520 525
Pro Ile Phe Ala Ala Ile Leu Thr Arg Ile Asn Glu Glu Arg Leu Ala
530 535 540
Ala Gly Met Pro Thr Val Gly Phe Val Asn Pro Met Leu Tyr Ala His
545 550 555 560
Pro Glu Ala Phe Arg Asp Ile Thr Glu Gly Asn Asn Pro Gly Cys Ser
565 570 575
Thr Asp Gly Phe Thr Ala Leu Glu Gly Trp Asp Pro Val Thr Gly Leu
580 585 590
Gly Thr Pro Ile Tyr Pro Lys Leu Leu Glu Val Phe Met Ser Val Lys
595 600 605
Leu Gly Leu
610
<210> 560
<211> 659
<212> PRT
<213> 产黄青霉
<400> 560
Met Ala Gly Tyr His Tyr Ser Phe Ile Val Ser Arg Asp Ala Ala Pro
1 5 10 15
Val Lys His Val Leu His Glu Lys Arg Ser Glu His Val Asp Trp Val
20 25 30
Lys Gly Glu Arg Ile Lys Arg Asp Ser Val Leu Pro Val Arg Ile Gly
35 40 45
Leu Ala Gln Asn Asn Leu Glu Arg Gly Asp Glu Tyr Leu Met Ala Val
50 55 60
Ser Asp Pro Asp Ser Ala Lys Tyr Gly Gln Tyr Trp Ser Ala Glu Glu
65 70 75 80
Val His Glu Phe Phe Ala Pro Ser Lys Asp Ser Val Asp Ser Val Arg
85 90 95
Arg Trp Leu Gln Ser Ser Gly Ile His Glu Ser Arg Ile Val His Ser
100 105 110
Asp Asn Lys Gly Trp Leu Ala Phe Asp Ala Tyr Ala His Glu Val Glu
115 120 125
Asp Leu Phe Lys Ala Glu Phe Tyr Glu His Glu His Ala Ser Thr Ser
130 135 140
Ser Met Lys Ile Gly Cys Glu Glu Tyr His Val Pro Glu Asn Ile Gln
145 150 155 160
Ala His Ile Asp Tyr Ile Thr Pro Gly Val Lys Leu Ser Pro Ile Val
165 170 175
Lys Lys Asn Thr Lys Ala Lys Arg Met Ser His Leu Ala His Ser Lys
180 185 190
Gly Thr Val Glu Ala Asp Asn Ser Ile Lys Thr Thr Val Ile Ser Glu
195 200 205
Lys Ala Gln Ser Leu Pro Ala Asp Leu Gln Arg Cys Gly Thr Asp Met
210 215 220
Thr Pro Ala Cys Ile Met Ala Leu Tyr Asp Ile Pro Asp Ala Thr Lys
225 230 235 240
Ala Ala Lys Gly Asn Ser Leu Gly Leu Tyr Glu Gln Gly Asp Tyr Phe
245 250 255
Ala Lys Ser Asp Leu Asp Leu Tyr Tyr Lys His Phe Ala Pro Trp Ile
260 265 270
Pro Arg Gly Thr Tyr Pro Ile Pro Ala Leu Ile Asp Gly Ala Ser Phe
275 280 285
Ser Val Pro Asp Tyr Ser Pro Leu Asn Ala Gly Glu Ala Asp Ile Asp
290 295 300
Ile Asp Met Ala Tyr Ser Leu Ile Tyr Pro Gln Ser Ile Thr Leu Tyr
305 310 315 320
Gln Val Asp Asp Gln Thr Tyr Glu Pro Glu Glu Glu Lys Thr Thr Asn
325 330 335
Leu Phe Asn Thr Phe Leu Asp Ala Leu Asp Gly Ser Tyr Cys Thr Tyr
340 345 350
Ser Ala Phe Gly Glu Thr Gly Asn Asn Pro Asp Ile Asp Pro Val Tyr
355 360 365
Pro Asn Pro Arg Pro Gly Gly Tyr Lys Gly Glu Leu Gln Cys Gly Val
370 375 380
Tyr Lys Pro Thr Thr Val Ile Ser Ala Ser Tyr Gly Gln Ala Glu Ala
385 390 395 400
Asp Leu Pro Ile Ala Tyr Thr Lys Arg Gln Cys Asn Glu Phe Met Lys
405 410 415
Leu Gly Leu Gln Gly His Ser Ile Leu Phe Ser Ser Gly Asp Tyr Gly
420 425 430
Val Ala Ser Phe His Asn Asp Gly Ala Val Asn Gly Cys Leu Gly Pro
435 440 445
Glu Gly Lys Ile Phe Asn Pro Gln Tyr Pro Ser Asn Cys Pro Tyr Val
450 455 460
Thr Ser Val Gly Gly Thr Met Leu Tyr Ala Asp Gln Thr Val Lys Asp
465 470 475 480
Ala Glu Ser Val Met Ser Ala Asn Leu Gly Gly Asp Ser Ala Asn Phe
485 490 495
Thr Ser Ser Gly Gly Phe Ser Asn Tyr Phe Pro Gln Pro Ser Tyr Gln
500 505 510
Lys Lys Ala Val Glu Lys Tyr Phe Gln Lys Ala Lys Leu Thr Tyr Pro
515 520 525
Tyr Tyr Ser Glu Met Glu Val Asn Ile Asn Lys Thr Lys Gly Leu Tyr
530 535 540
Asn Arg Ile Gly Arg Gly Tyr Pro Asp Val Ser Ala Asn Gly Ala Val
545 550 555 560
Phe Pro Ala Tyr Asn Gly Gly Lys Leu Thr His Tyr Tyr Gly Ala Ser
565 570 575
Leu Ala Ser Pro Leu Phe Ala Ser Val Leu Asn Leu Ile Asn Glu Glu
580 585 590
Arg Leu Asp Gln Gly Lys Gly Pro Val Gly Phe Val Asn Pro Val Leu
595 600 605
Tyr Ala His Pro Glu Val Leu Asn Asp Ile Thr Asn Gly Thr Asn Leu
610 615 620
Gly Cys Gly Ser Asp Gly Phe Ser Ala Ile Pro Gly Trp Asp Pro Ala
625 630 635 640
Thr Gly Leu Gly Thr Pro Asn Tyr Pro Lys Leu Lys Glu Leu Phe Leu
645 650 655
Ser Leu Pro
<210> 561
<211> 720
<212> PRT
<213> 嗜热毁丝霉
<400> 561
Met Arg Ile Arg His Ala Leu Val Gly Ile Ala Ser Leu Cys Cys Leu
1 5 10 15
Leu Gly Thr Ala Ser Gly Ala Arg Ile Ser Ser Arg Asp Met Leu Ser
20 25 30
Arg Arg Val Val Pro Pro Ser His Thr Leu His Glu Arg His Glu Ala
35 40 45
Gly Asn Val Glu Gly Trp Val Lys Arg Gly Leu Ala Asp Ala Glu Ser
50 55 60
Thr Val Pro Val Arg Ile Gly Leu Lys Gln Ser Asn Val Asp Ala Ala
65 70 75 80
His Asp Leu Leu Met Asp Ile Ser Asp Pro Arg Ser Pro Asn Tyr Gly
85 90 95
Lys His Leu Ser Arg Ser Glu Val Glu Asp Leu Phe Ala Pro Arg Glu
100 105 110
His Ser Val Ala Lys Val Lys Arg Trp Leu Ala Ser Ala Gly Val Asp
115 120 125
Glu Gly Arg Ile Ser Gln Ser Ala Asn Lys Gln Trp Ile Gln Phe Asp
130 135 140
Ala Pro Val Tyr Glu Leu Glu Lys Leu Leu Leu Thr Arg Tyr His Ile
145 150 155 160
Phe Glu Asn Leu Glu Thr Gly Val Gln Asn Ile Ala Cys Ser Glu Tyr
165 170 175
His Val Pro Arg Asp Val Ser His His Ile Asp Tyr Ile Thr Pro Gly
180 185 190
Ile Lys Leu Met Ala Gly Gly Arg Glu Glu Arg Met Val Arg Trp Arg
195 200 205
Lys Ala Asp Arg Arg Ser Leu Val Ala Gly Leu Ala Ser Gln Gly Arg
210 215 220
Lys Gly Ala His Gly Met Gly His Gly Gly Gly Gly Gly Ser Arg Ser
225 230 235 240
Pro Asp Asp Pro Val Val Asp Asp Ser Pro Phe Arg Val Thr Gly Pro
245 250 255
Cys Ser Ala Glu Ile Thr Pro Asn Cys Ile Arg Ala Gln Tyr Gln Leu
260 265 270
Pro Asn Gly Thr Arg Ala Ala Ser Gly Asn Glu Leu Gly Ile Phe Gln
275 280 285
Gly Leu Gly Gln His Tyr Ser Gln Glu Asp Leu Asp Asn Tyr Trp Lys
290 295 300
Tyr Val Ala Pro Trp Val Pro Arg Gly Thr His Pro Glu Leu Arg Ser
305 310 315 320
Ile Asn Gly Ala Leu Gly Pro Ala Asn Asp Thr Leu Arg Ala Gly Glu
325 330 335
Glu Ala Asp Leu Asp Phe Gln Ile Ala Ile Pro Leu Ile Trp Pro Gln
340 345 350
Arg Thr Val Leu Phe Gln Thr Asp Asp Glu Trp Tyr Gln Gln Asp Gln
355 360 365
Gln Arg Ala Asp Thr Lys Tyr Pro Gly Phe Phe Asn Thr Phe Phe Asp
370 375 380
Ala Ile Asp Gly Ser Tyr Cys His Met Thr Ala Phe Asn Met Thr Gly
385 390 395 400
Asn Cys Val Thr Pro Glu Cys Arg Asp Pro Glu Tyr Pro Asn Pro Asn
405 410 415
Ala Thr Pro Glu Gln Gly Gly Tyr Ala Gly Ala Leu Met Cys Gly Arg
420 425 430
His Arg Pro Thr Ser Val Val Ser Val Ser Tyr Ser Gly Thr Glu Asp
435 440 445
Ser Trp Pro Ala Ser Tyr Met Arg Arg Gln Cys Leu Glu Val Leu Lys
450 455 460
Leu Ala Leu Gln Gly Val Thr Val Val Glu Ser Ser Gly Asp Phe Gly
465 470 475 480
Val Gly Gly Arg Pro Phe Asp Pro Arg Ala Gly Cys Leu Gly Pro Asp
485 490 495
Arg Ala Val Phe Ser Pro Arg Val Met Ala Asn Cys Pro Tyr Val Leu
500 505 510
Ser Val Gly Ala Thr Ala Leu Val Asp Pro Glu Gln Glu Gln Gln Gln
515 520 525
Gln His Ala Asp Arg Gly Gly Ser Gly Lys Glu Pro Arg Leu Val Glu
530 535 540
Val Ala Ala Arg Thr Phe Ala Ser Gly Gly Gly Phe Ser Asn Ile Phe
545 550 555 560
Gly Arg Pro Lys Trp Gln Asp Arg His Val Arg Glu Tyr Leu Arg Lys
565 570 575
Thr Asn Leu Ser Glu Leu Gly Tyr Asp Asn Ala Ala Gly Met Ser Phe
580 585 590
Asp Ser Leu Arg Pro Pro Pro Ala Gly Gly Lys Leu Phe Asn Arg Leu
595 600 605
Gly Arg Gly Tyr Pro Asp Val Ala Ala Val Gly Gln Asn Phe Arg Val
610 615 620
Val Leu Arg Gly Tyr Pro Asn Arg Met His Gly Thr Ser Ala Ala Ala
625 630 635 640
Pro Val Trp Ala Ser Ile Leu Thr Leu Ile Asn Glu Glu Arg Arg Ala
645 650 655
Val Gly Lys Gly Pro Val Gly Phe Val His Gln Val Leu Tyr Gln His
660 665 670
Pro Glu Val Phe Thr Asp Ile Thr Val Gly Ser Asn Pro Gly Cys Gly
675 680 685
Thr Asp Gly Phe Pro Val Glu Glu Gly Trp Asp Pro Val Thr Gly Leu
690 695 700
Gly Ser Pro Ile Tyr Pro Lys Leu Leu Lys Leu Phe Met Ser Leu Pro
705 710 715 720
<210> 562
<211> 719
<212> PRT
<213> 粗糙链孢霉
<400> 562
Met Phe Arg Phe His Leu Trp Thr Leu Leu Arg Leu Phe Ala Leu Leu
1 5 10 15
Ser Ser Leu Val Thr Ala Ser Arg Ile Val Leu Glu Glu Ala Gly His
20 25 30
Leu Pro Ala Gly Trp Lys Val Glu Arg His Ala Thr Ala Ser Asp Arg
35 40 45
Ile Gln Leu Ser Ile Ala Leu Lys Glu Pro Gly Ile Glu Glu Leu Lys
50 55 60
Arg Arg Leu Leu Gln Gln Ser Thr Ser Asp Asp His Pro Asn Ser Arg
65 70 75 80
Gln Phe Thr Lys Glu Glu Val Glu Lys His Arg Gln Pro Asp Gln Arg
85 90 95
Ser Val Thr Ala Val Gly Arg Trp Leu Gln Ser His Gly Ile Lys Ser
100 105 110
Tyr Asn Ala Asp Asn Ser Trp Ile Thr Phe Lys Ala Thr Ala Ala Thr
115 120 125
Val Gln Met Leu Phe Glu Ala Asp Leu Ala Tyr Tyr Ser Tyr Asn Gly
130 135 140
Asp Pro Ser Thr Gln Ile Leu Arg Ser Arg Ser Tyr Thr Ile Pro Arg
145 150 155 160
Trp Leu Ser Asp Asp Ile Asp Phe Val His Pro Leu Thr Asn Phe Met
165 170 175
Pro Pro Arg Asn Arg Asn Asp Gly Thr Leu Gly Ile Gly Arg Arg Gln
180 185 190
Pro Ile Gln Pro Lys Leu Ser Ala Arg Glu Asp Phe Phe Ala Pro Pro
195 200 205
Cys Trp Thr Gly Thr Phe Pro Gly Cys Ile Arg Lys Leu Tyr Asn Leu
210 215 220
Thr Tyr Thr Pro Ser Pro Asp Phe Arg Ser Pro Ser Pro Val Arg Phe
225 230 235 240
Gly Ile Ala Ser Phe Leu Glu Gln Tyr Ile Thr His Arg Asp Val Thr
245 250 255
Ser Phe Leu Ala Thr Tyr Ala Arg Glu Leu Leu Pro Leu Arg Pro Thr
260 265 270
Pro Ser Arg Gly Gly Ser Gly Gly Ser Leu Thr Leu Pro Pro Val Thr
275 280 285
Asn Thr Thr Ser Glu Pro Pro Tyr Asn Ile Thr Ile Thr Leu Leu Asn
290 295 300
Asn Ala Thr Arg Trp Asp Pro His Ser Thr Asp Pro Ala Leu Ser Gly
305 310 315 320
Leu Glu Ala Asn Leu Asp Val Gln Tyr Ala Leu Ser Leu Gly His Pro
325 330 335
Thr Arg Val Ile Tyr Tyr Ala Thr Gly Gly Arg Gly Thr Lys Leu Asp
340 345 350
Ser Ser Gly Arg Pro Leu Pro Thr Asn Asp Pro Arg Ala Asn Asn Glu
355 360 365
Pro Phe Leu Glu Phe Leu Gln Ala Leu Leu Ala Leu Pro Asp Asn Gln
370 375 380
Ile Pro His Val Leu Ser Ile Ser Tyr Ala Asp Asp Glu Gln Ser Val
385 390 395 400
Pro Arg Lys Tyr Ala His Arg Val Cys Asp Leu Phe Ala Ala Val Ala
405 410 415
Ala Arg Gly Thr Ser Val Leu Val Ala Thr Gly Asp Gly Gly Ala Ala
420 425 430
Gly Ile Gly Phe Ser Ala Gly Gly Gly Asp Thr Cys Ile Lys Asn Asp
435 440 445
Gly Ser Gly Arg Arg Ala Phe Val Pro Thr Phe Pro Ala Ser Cys Pro
450 455 460
Trp Val Thr Ser Val Gly Ala Thr Asp Asn Thr Ala Leu Asn Leu Thr
465 470 475 480
Gly Ala Ala Phe Ser Ser Gly Gly Phe Ser Glu Tyr Phe Asp Arg Pro
485 490 495
Leu Trp Gln Arg Ala Ala Val Asp Pro Tyr Val Ser Ser Leu Leu Arg
500 505 510
Ser Arg Ser Ser Lys Pro Gly Gln Pro Ser Gln Pro Arg Asp Leu Lys
515 520 525
Gly Val Tyr Phe Ser His Asn Gly Arg Gly Met Pro Asp Met Ala Ala
530 535 540
Ile Gly Ser Gly Phe Gln Ile Ile His Arg Gly Glu Met Val Glu Val
545 550 555 560
Arg Gly Thr Ser Ala Ser Thr Pro Val Val Ala Ala Met Val Ala Leu
565 570 575
Val Asn Asp Gln Arg Leu Arg Gln Gly Lys Arg Ser Leu Gly Trp Leu
580 585 590
Asn Gly His Leu Tyr Leu Asp Pro Arg Val Arg Arg Val Leu Thr Asp
595 600 605
Val Lys Trp Gly Arg Ser Glu Gly Cys Val Phe Pro Gly Glu Ala Leu
610 615 620
Glu Glu Gly Arg Gly Lys Gly Lys Glu Lys Tyr Trp Arg His Ser Val
625 630 635 640
Val Glu Lys Arg Gln Gly Asn Ser Glu Glu Asp Gly Gly Thr His Gly
645 650 655
Gly Asp Gly Glu Gly Lys Ala Asp Glu Glu Asp Trp Gly Gly Glu Gly
660 665 670
Glu Val Gly Glu Gly Glu Gly Asp Gln Ser Glu Asn Val Ile Leu Gly
675 680 685
Gly Trp Asp Ala Arg Lys Gly Trp Asp Pro Val Thr Gly Leu Gly Val
690 695 700
Pro Gly Asp Phe Gln Glu Met Leu Lys Val Leu Gly Ser Val Trp
705 710 715
<210> 563
<211> 639
<212> PRT
<213> 黑曲霉
<400> 563
Met His Val Ser Leu Phe Leu Leu Ser Val Thr Ala Ala Phe Ala Ser
1 5 10 15
Pro Thr Pro His Asn Tyr Val Val His Glu Arg Arg Asp Ala Leu Pro
20 25 30
Ser Val Trp Val Glu Glu Ser Arg Leu Asp Lys Gly Ala Leu Leu Pro
35 40 45
Met Arg Ile Gly Leu Thr Gln Ser Asn Leu Asp Arg Gly His Asp Leu
50 55 60
Leu Met Glu Val Ser His Pro Gln Ser Ser Arg Tyr Gly Lys His Leu
65 70 75 80
Ser Ser Glu Glu Val His Asp Leu Phe Ala Pro Ser Asn Glu Ala Val
85 90 95
Glu Thr Val Arg Thr Trp Ile Glu Ser Ala Gly Ile Ala Pro Ser Arg
100 105 110
Ile Ser Gln Ser Tyr Asn Lys Gln Trp Leu Gln Phe Asp Ala His Ala
115 120 125
Ser Glu Val Glu Gln Leu Leu Gln Thr Glu Tyr Tyr Ile Tyr Thr His
130 135 140
Ala Asp Thr Gly Ser Ser His Val Thr Cys His Glu Tyr His Val Pro
145 150 155 160
Glu Thr Ile Gln Ser His Ile Asp Tyr Ile Thr Pro Gly Val Lys Met
165 170 175
Leu Glu Val Arg Gly Thr Pro Ser Lys Lys Arg Asp Ala Glu Lys Arg
180 185 190
Ser Leu Gly Ser Leu Pro Pro Ile Leu Ala Pro Leu Pro Ile Asn Ile
195 200 205
Thr Lys Ile Phe Asp Asp Pro Leu Ala His Cys Asp Leu Ala Val Thr
210 215 220
Pro Asp Cys Ile Arg Ala Met Tyr Asn Ile Thr Lys Gly Thr Thr Ala
225 230 235 240
Thr Lys Gly Asn Glu Leu Gly Ile Phe Glu Asp Leu Gly Asp Ile Tyr
245 250 255
Ser Gln Asp Asp Leu Asn Leu Phe Phe Ala Asn Phe Ala Ser Asp Ile
260 265 270
Pro Gln Gly Thr His Pro Thr Leu Asp Ser Ile Asp Gly Ala Thr Ala
275 280 285
Pro Thr Asp Val Thr Asn Ala Gly Pro Glu Ser Asp Leu Asp Phe Gln
290 295 300
Ile Ala Tyr Pro Ile Ile Trp Pro Gln Asn Thr Ile Leu Tyr Gln Thr
305 310 315 320
Asp Asp Pro Asn Tyr Glu Asp Asn Tyr Asn Phe Lys Gly Leu Leu Asn
325 330 335
Asn Phe Leu Tyr Ala Ile Asp Gly Ser Tyr Cys Asn Glu Thr Ser Ser
340 345 350
Leu Asp Pro Gln Tyr Pro Asp Pro Ser Pro Gly Gly Tyr Ser Ser Pro
355 360 365
Lys Gln Cys Gly Val Tyr Thr Pro Thr Asn Val Ile Ser Ile Ser Tyr
370 375 380
Gly Ser Pro Glu Ala Asp Leu Pro Ile Ala Tyr Gln Arg Arg Gln Cys
385 390 395 400
His Glu Phe Met Lys Leu Gly Leu Gln Gly Ile Ser Val Val Val Ala
405 410 415
Ser Gly Asp Ser Gly Val Ala Ser Ser Thr Gly Thr Cys Phe Gly Asp
420 425 430
Ala Asp Asn Val Phe Val Pro Asp Phe Pro Ala Thr Cys Pro Tyr Leu
435 440 445
Thr Ala Val Gly Gly Thr Tyr Leu Pro Leu Gly Ala Asp Ala Ala Lys
450 455 460
Asp Gln Glu Ile Ala Val Thr Arg Phe Pro Ser Gly Gly Gly Phe Ser
465 470 475 480
Asn Ile Tyr Ala Arg Pro Ser Tyr Gln Asn His Ser Val Glu Thr Tyr
485 490 495
Phe Ser Thr Thr Ser Asp Asp Leu Thr Tyr Pro Tyr Tyr Ser Gly Val
500 505 510
Asn Tyr Thr Asp Phe Ser Asn Thr Asp Gly Val Tyr Asn Arg Ile Gly
515 520 525
Arg Gly Tyr Pro Asp Val Ser Ala Ile Ala Asp Asn Ile Ile Ile Tyr
530 535 540
Asn Gln Gly Glu Ala Thr Leu Val Gly Gly Thr Ser Ala Ala Ala Pro
545 550 555 560
Ala Phe Ala Ala Met Leu Thr Arg Ile Asn Glu Glu Arg Leu Ala Lys
565 570 575
Gly Lys Ser Thr Val Gly Phe Val Asn Pro Val Leu Tyr Glu His Pro
580 585 590
Glu Ala Phe Arg Asp Val Thr Val Gly Ser Asn Pro Gly Cys Gly Thr
595 600 605
Asp Gly Phe Pro Val Ala Gly Gly Trp Asp Pro Val Thr Gly Leu Gly
610 615 620
Thr Pro Arg Phe Glu Asp Leu Met Asp Ile Phe Val Gly Asp Asp
625 630 635
<210> 564
<211> 602
<212> PRT
<213> 烟曲霉
<400> 564
Met Phe Ser Ser Leu Leu Asn Arg Gly Ala Leu Leu Ala Val Val Ser
1 5 10 15
Leu Leu Ser Ser Ser Val Ala Ala Glu Val Phe Glu Lys Leu Ser Ala
20 25 30
Val Pro Gln Gly Trp Lys Tyr Ser His Thr Pro Ser Asp Arg Asp Pro
35 40 45
Ile Arg Leu Gln Ile Ala Leu Lys Gln His Asp Val Glu Gly Phe Glu
50 55 60
Thr Ala Leu Leu Glu Met Ser Asp Pro Tyr His Pro Asn Tyr Gly Lys
65 70 75 80
His Phe Gln Thr His Glu Glu Met Lys Arg Met Leu Leu Pro Thr Gln
85 90 95
Glu Ala Val Glu Ser Val Arg Gly Trp Leu Glu Ser Ala Gly Ile Ser
100 105 110
Asp Ile Glu Glu Asp Ala Asp Trp Ile Lys Phe Arg Thr Thr Val Gly
115 120 125
Val Ala Asn Asp Leu Leu Asp Ala Asp Phe Lys Trp Tyr Val Asn Glu
130 135 140
Val Gly His Val Glu Arg Leu Arg Thr Leu Ala Tyr Ser Leu Pro Gln
145 150 155 160
Ser Val Ala Ser His Val Asn Met Val Gln Pro Thr Thr Arg Phe Gly
165 170 175
Gln Ile Lys Pro Asn Arg Ala Thr Met Arg Gly Arg Pro Val Gln Val
180 185 190
Asp Ala Asp Ile Leu Ser Ala Ala Val Gln Ala Gly Asp Thr Ser Thr
195 200 205
Cys Asp Gln Val Ile Thr Pro Gln Cys Leu Lys Asp Leu Tyr Asn Ile
210 215 220
Gly Asp Tyr Lys Ala Asp Pro Asn Gly Gly Ser Lys Val Ala Phe Ala
225 230 235 240
Ser Phe Leu Glu Glu Tyr Ala Arg Tyr Asp Asp Leu Ala Lys Phe Glu
245 250 255
Glu Lys Leu Ala Pro Tyr Ala Ile Gly Gln Asn Phe Ser Val Ile Gln
260 265 270
Tyr Asn Gly Gly Leu Asn Asp Gln Asn Ser Ala Ser Asp Ser Gly Glu
275 280 285
Ala Asn Leu Asp Leu Gln Tyr Ile Val Gly Val Ser Ser Pro Ile Pro
290 295 300
Val Thr Glu Phe Ser Thr Gly Gly Arg Gly Leu Leu Ile Pro Asp Leu
305 310 315 320
Ser Gln Pro Asp Pro Asn Asp Asn Ser Asn Glu Pro Tyr Leu Glu Phe
325 330 335
Leu Gln Asn Val Leu Lys Met Asp Gln Asp Lys Leu Pro Gln Val Ile
340 345 350
Ser Thr Ser Tyr Gly Glu Asp Glu Gln Thr Ile Pro Glu Lys Tyr Ala
355 360 365
Arg Ser Val Cys Asn Leu Tyr Ala Gln Leu Gly Ser Arg Gly Val Ser
370 375 380
Val Ile Phe Ser Ser Gly Asp Ser Gly Val Gly Ala Ala Cys Leu Thr
385 390 395 400
Asn Asp Gly Thr Asn Arg Thr His Phe Pro Pro Gln Phe Pro Ala Ala
405 410 415
Cys Pro Trp Val Thr Ser Val Gly Gly Thr Thr Lys Thr Gln Pro Glu
420 425 430
Glu Ala Val Tyr Phe Ser Ser Gly Gly Phe Ser Asp Leu Trp Glu Arg
435 440 445
Pro Ser Trp Gln Asp Ser Ala Val Lys Arg Tyr Leu Lys Lys Leu Gly
450 455 460
Pro Arg Tyr Lys Gly Leu Tyr Asn Pro Lys Gly Arg Ala Phe Pro Asp
465 470 475 480
Val Ala Ala Gln Ala Glu Asn Tyr Ala Val Phe Asp Lys Gly Val Leu
485 490 495
His Gln Phe Asp Gly Thr Ser Cys Ser Ala Pro Ala Phe Ser Ala Ile
500 505 510
Val Ala Leu Leu Asn Asp Ala Arg Leu Arg Ala His Lys Pro Val Met
515 520 525
Gly Phe Leu Asn Pro Trp Leu Tyr Ser Lys Ala Ser Lys Gly Phe Asn
530 535 540
Asp Ile Val Lys Gly Gly Ser Lys Gly Cys Asp Gly Arg Asn Arg Phe
545 550 555 560
Gly Gly Thr Pro Asn Gly Ser Pro Val Val Pro Tyr Ala Ser Trp Asn
565 570 575
Ala Thr Asp Gly Trp Asp Pro Ala Thr Gly Leu Gly Thr Pro Asp Phe
580 585 590
Gly Lys Leu Leu Ser Leu Ala Met Arg Arg
595 600
<210> 565
<211> 611
<212> PRT
<213> 黑曲霉
<400> 565
Met Leu Ser Ser Leu Leu Ser Gln Gly Ala Ala Val Ser Leu Ala Val
1 5 10 15
Leu Ser Leu Leu Pro Ser Pro Val Ala Ala Glu Ile Phe Glu Lys Leu
20 25 30
Ser Gly Val Pro Asn Gly Trp Arg Tyr Ala Asn Asn Pro Gln Gly Asn
35 40 45
Glu Val Ile Arg Leu Gln Ile Ala Leu Gln Gln His Asp Val Ala Gly
50 55 60
Phe Glu Gln Ala Val Met Asp Met Ser Thr Pro Gly His Ala Asp Tyr
65 70 75 80
Gly Lys His Phe Arg Thr His Asp Glu Met Lys Arg Met Leu Leu Pro
85 90 95
Ser Glu Thr Ala Val Asp Ser Val Arg Asp Trp Leu Glu Ser Ala Gly
100 105 110
Val His Asn Ile Gln Val Asp Ala Asp Trp Val Lys Phe His Thr Thr
115 120 125
Val Asn Lys Ala Asn Ala Leu Leu Asp Ala Asp Phe Lys Trp Tyr Val
130 135 140
Ser Asp Ala Lys His Ile Arg Arg Leu Arg Thr Leu Gln Tyr Ser Ile
145 150 155 160
Pro Asp Ala Leu Val Ser His Ile Asn Met Ile Gln Pro Thr Thr Arg
165 170 175
Phe Gly Gln Ile Gln Pro Asn Arg Ala Thr Met Arg Ser Lys Pro Lys
180 185 190
His Ala Asp Glu Thr Phe Leu Thr Ala Ala Thr Leu Ala Gln Asn Thr
195 200 205
Ser His Cys Asp Ser Ile Ile Thr Pro His Cys Leu Lys Gln Leu Tyr
210 215 220
Asn Ile Gly Asp Tyr Gln Ala Asp Pro Lys Ser Gly Ser Lys Ile Gly
225 230 235 240
Phe Ala Ser Tyr Leu Glu Glu Tyr Ala Arg Tyr Ala Asp Leu Glu Arg
245 250 255
Phe Glu Gln His Leu Ala Pro Asn Ala Ile Gly Gln Asn Phe Ser Val
260 265 270
Val Gln Phe Asn Gly Gly Leu Asn Asp Gln Leu Ser Ser Ser Asp Ser
275 280 285
Gly Glu Ala Asn Leu Asp Leu Gln Tyr Ile Leu Gly Val Ser Ala Pro
290 295 300
Val Pro Ile Thr Glu Tyr Ser Thr Gly Gly Arg Gly Glu Leu Val Pro
305 310 315 320
Asp Leu Ser Ser Pro Asp Pro Asn Asp Asn Ser Asn Glu Pro Tyr Leu
325 330 335
Asp Phe Leu Gln Gly Ile Leu Lys Leu Asn Asn Ser Asp Leu Pro Gln
340 345 350
Val Ile Ser Thr Ser Tyr Gly Glu Asp Glu Gln Thr Ile Pro Val Pro
355 360 365
Tyr Ala Arg Thr Val Cys Asn Leu Tyr Ala Gln Leu Gly Ser Arg Gly
370 375 380
Val Ser Val Ile Phe Ser Ser Gly Asp Ser Gly Val Gly Ala Ala Cys
385 390 395 400
Leu Thr Asn Asp Gly Thr Asn Arg Thr His Phe Pro Pro Gln Phe Pro
405 410 415
Ala Ser Cys Pro Trp Val Thr Ser Val Gly Ala Thr Ser Lys Thr Ser
420 425 430
Pro Glu Gln Ala Val Ser Phe Ser Ser Gly Gly Phe Ser Asp Leu Trp
435 440 445
Pro Arg Pro Ser Tyr Gln His Ala Ala Val Gln Thr Tyr Leu Thr Lys
450 455 460
His Leu Gly Asn Lys Phe Ser Gly Leu Phe Asn Ala Ser Gly Arg Ala
465 470 475 480
Phe Pro Asp Val Ser Ala Gln Gly Val Asn Tyr Ala Val Tyr Asp Lys
485 490 495
Gly Met Leu Gly Gln Phe Asp Gly Thr Ser Cys Ser Ala Pro Thr Phe
500 505 510
Ser Gly Val Ile Ala Leu Leu Asn Asp Ala Arg Leu Arg Ala Gly Leu
515 520 525
Pro Val Met Gly Phe Leu Asn Pro Phe Leu Tyr Gly Val Gly Ser Glu
530 535 540
Lys Gly Ala Leu Asn Asp Ile Val Asn Gly Gly Ser Val Gly Cys Asp
545 550 555 560
Gly Arg Asn Arg Phe Gly Gly Thr Pro Asn Gly Ser Pro Val Val Pro
565 570 575
Phe Ala Ser Trp Asn Ala Thr Thr Gly Trp Asp Pro Val Ser Gly Leu
580 585 590
Gly Thr Pro Asp Phe Ala Lys Leu Lys Gly Val Ala Leu Gly Glu Glu
595 600 605
Gly Gly Asn
610
<210> 566
<211> 596
<212> PRT
<213> 烟曲霉
<400> 566
Met Ala Pro Phe Thr Phe Leu Val Gly Ile Leu Ser Leu Cys Ile Cys
1 5 10 15
Cys Ile Val Leu Gly Ala Ala Ala Glu Pro Ser Tyr Ala Val Val Glu
20 25 30
Gln Leu Arg Asn Val Pro Asp Gly Trp Ile Lys His Asp Ala Ala Pro
35 40 45
Ala Ser Glu Leu Ile Arg Phe Arg Leu Ala Met Asn Gln Glu Arg Ala
50 55 60
Ala Glu Phe Glu Arg Arg Val Ile Asp Met Ser Thr Pro Gly His Ser
65 70 75 80
Ser Tyr Gly Gln His Met Lys Arg Asp Asp Val Arg Glu Phe Leu Arg
85 90 95
Pro Pro Glu Glu Val Ser Asp Lys Val Leu Ser Trp Leu Arg Ser Glu
100 105 110
Asn Val Pro Ala Gly Ser Ile Glu Ser His Gly Asn Trp Val Thr Phe
115 120 125
Thr Val Pro Val Ser Gln Ala Glu Arg Met Leu Arg Thr Arg Phe Tyr
130 135 140
Ala Phe Gln His Val Glu Thr Ser Thr Thr Gln Val Arg Thr Leu Ala
145 150 155 160
Tyr Ser Val Pro His Asp Val His Arg Tyr Ile Gln Met Ile Gln Pro
165 170 175
Thr Thr Arg Phe Gly Gln Pro Ala Arg His Glu Arg Gln Pro Leu Phe
180 185 190
His Gly Thr Val Ala Thr Lys Glu Glu Leu Ala Ala Asn Cys Ser Thr
195 200 205
Thr Ile Thr Pro Asn Cys Leu Arg Glu Leu Tyr Gly Ile Tyr Asp Thr
210 215 220
Arg Ala Glu Pro Asp Pro Arg Asn Arg Leu Gly Val Ser Gly Phe Leu
225 230 235 240
Asp Gln Tyr Ala Arg Tyr Asp Asp Phe Glu Asn Phe Met Arg Leu Tyr
245 250 255
Ala Thr Ser Arg Thr Asp Val Asn Phe Thr Val Val Ser Ile Asn Asp
260 265 270
Gly Leu Asn Leu Gln Asp Ser Ser Leu Ser Ser Thr Glu Ala Ser Leu
275 280 285
Asp Val Gln Tyr Ala Tyr Ser Leu Ala Tyr Lys Ala Leu Gly Thr Tyr
290 295 300
Tyr Thr Thr Gly Gly Arg Gly Pro Val Val Pro Glu Glu Gly Gln Asp
305 310 315 320
Thr Asn Val Ser Thr Asn Glu Pro Tyr Leu Asp Gln Leu His Tyr Leu
325 330 335
Leu Asp Leu Pro Asp Glu Glu Leu Pro Ala Val Leu Ser Thr Ser Tyr
340 345 350
Gly Glu Asp Glu Gln Ser Val Pro Glu Ser Tyr Ser Asn Ala Thr Cys
355 360 365
Asn Leu Phe Ala Gln Leu Gly Ala Arg Gly Val Ser Ile Ile Phe Ser
370 375 380
Ser Gly Asp Ser Gly Val Gly Ser Thr Cys Ile Thr Asn Asp Gly Thr
385 390 395 400
Lys Thr Thr Arg Phe Leu Pro Val Phe Pro Ala Ser Cys Pro Phe Val
405 410 415
Thr Ala Val Gly Gly Thr His Asp Ile Gln Pro Glu Lys Ala Ile Ser
420 425 430
Phe Ser Ser Gly Gly Phe Ser Asp His Phe Pro Arg Pro Ser Tyr Gln
435 440 445
Asp Ser Ser Val Gln Gly Tyr Leu Glu Gln Leu Gly Ser Arg Trp Asn
450 455 460
Gly Leu Tyr Asn Pro Ser Gly Arg Gly Phe Pro Asp Val Ala Ala Gln
465 470 475 480
Ala Thr Asn Phe Val Val Ile Asp His Gly Gln Thr Leu Arg Val Gly
485 490 495
Gly Thr Ser Ala Ser Ala Pro Val Phe Ala Ala Ile Val Ser Arg Leu
500 505 510
Asn Ala Ala Arg Leu Glu Asp Gly Leu Leu Lys Leu Gly Phe Leu Asn
515 520 525
Pro Trp Leu Tyr Ser Leu Asn Gln Thr Gly Phe Thr Asp Ile Ile Asp
530 535 540
Gly Gly Ser Ser Gly Cys Tyr Val Gly Thr Ser Asn Glu Gln Leu Val
545 550 555 560
Pro Asn Ala Ser Trp Asn Ala Thr Pro Gly Trp Asp Pro Val Thr Gly
565 570 575
Leu Gly Thr Pro Ile Tyr Asn Thr Leu Val Lys Leu Ala Thr Ser Val
580 585 590
Ser Ser Thr Pro
595
<210> 567
<211> 595
<212> PRT
<213> 黄曲霉
<400> 567
Met Pro Ser Gln Gln Met Val Leu Phe Ala Tyr Thr Ile Gly Ala Phe
1 5 10 15
Ser Leu Val Leu His Leu Leu Val Ala Ala Leu Ala Thr Gly Pro Arg
20 25 30
Tyr Ser Val Val Glu Arg Leu Glu Lys Ile Pro Asp Gly Trp Val Asp
35 40 45
Val Gly Thr Pro Ser Pro Ser Lys Pro Ile Glu Leu Gln Leu Ala Val
50 55 60
His Pro Glu Lys Ile Thr Glu Phe Glu Gln Arg Val Ile Glu Ile Ser
65 70 75 80
Thr Pro Gly Asn Gln Asn Tyr Gly Arg His Met Ser Arg Asp Glu Val
85 90 95
Arg Asn Phe Leu Tyr Ser Ser Asp Val Ala Pro Arg Lys Val Leu Ser
100 105 110
Trp Leu Lys Ala Ala His Ile Pro Ala Thr Ser Ile Asn Lys His Thr
115 120 125
Gly Trp Ile Thr Phe Thr Val Pro Val Ser Gln Ala Glu Gln Leu Leu
130 135 140
Arg Thr Arg Phe Tyr Thr Phe Glu His Lys Lys Ser Arg Ala Thr Val
145 150 155 160
Val Arg Thr Leu Lys Tyr Ser Val Pro Lys Glu Leu Arg Ser Phe Val
165 170 175
Gln Met Ile Gln Pro Thr Thr Arg Phe Gly Gln Leu Met Pro Gln Thr
180 185 190
Glu Pro Pro Leu Leu Gln Ala Leu Pro Val Thr Leu Lys Asp Leu Val
195 200 205
Ala Gly Cys Ser Ser Ile Val Thr Pro Ser Cys Leu Arg Lys Leu Tyr
210 215 220
Gly Ile Ser Asp Ser Lys Pro Lys Pro Asp Arg Arg Asn Arg Leu Gly
225 230 235 240
Val Ser Gly Phe Leu Asp Gln Tyr Ala Arg Tyr Ser Asp Phe His Gln
245 250 255
Phe Leu Arg Leu Tyr Ala Pro Asn Ile Thr Asp Asn Asn Tyr Thr Val
260 265 270
Glu Leu Ile Asn Gly Gly Leu Asn Leu Gln Asp Ser Leu Glu Asp Ser
275 280 285
Ser Glu Ala Ser Leu Asp Ile Gln Tyr Ala Ala Ala Leu Ala Asp Thr
290 295 300
Pro Phe Thr Thr Phe Tyr Ser Thr Ala Gly Arg Gly Pro Val Ile Arg
305 310 315 320
Asp Leu Gly His Asn Asp Pro Ser Asp Pro His Glu Pro Tyr Leu Glu
325 330 335
Gln Leu Arg Tyr Leu Leu Asp Leu Pro Asp Asp Glu Leu Pro Ala Val
340 345 350
Leu Thr Thr Ser Tyr Gly Glu Leu Glu Gln Ser Val Pro Glu Ile Tyr
355 360 365
Ala Arg Thr Thr Cys Asn Met Phe Ala Gln Leu Gly Ala Arg Gly Val
370 375 380
Ser Val Ile Phe Ser Ser Gly Asp Ser Gly Val Gly Asp Ser Cys Ile
385 390 395 400
Thr Asn Asp Gly Thr Asn Arg Thr Arg Phe Gln Pro Leu Tyr Pro Ala
405 410 415
Ser Cys Pro Phe Val Thr Ser Val Gly Gly Thr Tyr Gly Lys Glu Pro
420 425 430
Glu Met Ala Ile Gly Tyr Ser Thr Gly Gly Phe Ser Glu Tyr Phe Pro
435 440 445
Arg Pro Ser Tyr Gln Asp Gly Ser Val Asn Gln Tyr Leu Ser Gln Leu
450 455 460
Gly Ser Lys Trp Glu Gly Leu Tyr Asn Pro Asn Gly Arg Ala Ile Pro
465 470 475 480
Asp Val Ala Ala Gln Ala Asp His Phe Ile Ile Met Asp His Gly Gln
485 490 495
Leu Leu Lys Thr Gly Gly Thr Ser Ala Ala Ala Pro Val Phe Ala Gly
500 505 510
Ile Val Ser Arg Leu Asn Ala Ala Arg Leu Glu Ser Asn Lys Pro Arg
515 520 525
Leu Gly Phe Leu Asn Pro Trp Leu Tyr Ser Leu Asn Gln Thr Gly Phe
530 535 540
Thr Asp Ile Val Asp Gly Gly Ser Val Gly Cys Phe Gly Asp Val Gly
545 550 555 560
Arg Glu Val Pro Tyr Ala Ser Trp Asn Ala Thr Pro Gly Trp Asp Pro
565 570 575
Val Thr Gly Leu Gly Thr Pro Tyr Tyr Lys Ala Leu Val Glu Gln Ala
580 585 590
Met Ser Ile
595
<210> 568
<211> 1068
<212> PRT
<213> 构巢曲霉
<400> 568
Met Val Ser Leu Ser Phe Leu Ala Gly Thr Leu Ser Leu Val Ile Ala
1 5 10 15
Cys Phe Val Asn Val Val Ala Ala Asp Phe Gly Tyr Val Thr Val Glu
20 25 30
Arg Leu Gln Arg Ala Pro Asp Gly Trp Ile Arg Gly Pro Val Ala Pro
35 40 45
Pro Phe Lys Met Ile Lys Phe His Ile Ala Leu Arg Gln Glu Lys Ala
50 55 60
Ala Glu Leu Glu Gln Val Thr Leu Asp Met Ser Thr Pro Gly His His
65 70 75 80
Asn Tyr Gly Arg His Leu Lys Arg Asp Asp Val Arg Ala Phe Val Arg
85 90 95
Pro Ser Gly Glu Thr Met Asn Arg Val Leu Ala Trp Leu Glu Ser Ala
100 105 110
Glu Ile Pro Ala Glu Ser Val Arg Thr His Gly Asn Trp Met Glu Val
115 120 125
Ile Leu Pro Val Ser Arg Ala Glu Ser Leu Met Lys Thr Lys Phe His
130 135 140
Ile Tyr Thr His Arg Asp Thr His Arg Arg Ala Ile Arg Thr Leu Ser
145 150 155 160
Tyr Ser Val Pro Arg Ala Leu Phe Pro Asp Ile Gln Leu Ile Gln Pro
165 170 175
Thr Thr His Phe Gly Gln Ala Val Ala Gln Gly Lys Arg Pro Asn Phe
180 185 190
Gln Pro Val Ala Ala Thr Phe Asp Arg Leu Thr Ala Asp Cys Ser Thr
195 200 205
Thr Ile Thr Pro Asp Cys Leu Arg Glu Leu Tyr Gly Leu Tyr Asp Thr
210 215 220
Asn Ala Lys Pro Asp Val Arg Asn Arg Leu Gly Ile Ser Gly Tyr Leu
225 230 235 240
Glu Glu Tyr Ala Arg Tyr Ser Asp Phe His Gln Phe Met His Gln Phe
245 250 255
Ser Pro Asn Arg Thr Glu Ala Asn Phe Ser Val Val Ser Ile Asn Gly
260 265 270
Gly Leu Asn Leu Gln Asn Ser Ser Leu Pro Ser Ser Glu Ala Ser Leu
275 280 285
Asp Val Gln Tyr Ala Ile Ser Leu Ala Tyr Asn Thr Phe Ala Thr Tyr
290 295 300
Tyr Thr Thr Ala Gly Arg Ala Pro Phe Leu Pro Gly Ala Thr Gly Thr
305 310 315 320
Asp Glu Asp Ile Ser Ile Asn Glu Pro Tyr Leu Glu Gln Leu His Tyr
325 330 335
Leu Leu Asp Leu Pro Asp Glu Glu Leu Pro Ala Val Leu Thr Thr Ser
340 345 350
Tyr Gly Glu Asp Glu Gln Ser Val Pro Glu Ser Tyr Ala Glu Ala Thr
355 360 365
Cys Asn Leu Phe Ala Gln Leu Gly Ala Arg Gly Val Ser Val Ile Phe
370 375 380
Ser Ser Gly Asp Gly Gly Val Gly Gly Ser Cys Leu Ala Asn Asp Gly
385 390 395 400
Thr Asn Arg Thr Arg Phe Gln Pro Ile Phe Pro Ala Ser Cys Pro Phe
405 410 415
Val Thr Ser Val Gly Gly Thr Glu Gly Met Asn Pro Glu Arg Ala Val
420 425 430
Glu Phe Ser Gly Gly Gly Phe Ser Asp Arg Phe Ala Arg Pro Ser Tyr
435 440 445
Gln Asp Ser Ser Val Leu Gly Phe Leu Lys Thr Leu Gly Gly Lys Trp
450 455 460
Asp Gly Leu Tyr Asn Pro Gln Gly Arg Gly Ile Pro Asp Val Ala Ala
465 470 475 480
Gln Ala Asn Asn Tyr Ile Ile Ile Asp His Gly Lys Thr Tyr His Ile
485 490 495
Gly Gly Thr Ser Ala Ser Ala Pro Val Phe Ala Ala Ile Val Ser Arg
500 505 510
Leu Asn Ala Ala Arg Leu Glu Asp Gly Lys Pro Arg Leu Gly Phe Leu
515 520 525
Asn Pro Trp Leu Tyr Ser Leu Asn Gln Thr Gly Phe Thr Asp Ile Val
530 535 540
Asp Gly Arg Ser Pro Arg Pro Ala Cys Val Ser Met Lys Thr Arg Pro
545 550 555 560
Gly Ser Ser Ala Trp Ala Lys Ala Ile Ser Ala Asp Pro Lys Thr Lys
565 570 575
Arg Gly Leu Thr His Arg Ala Pro Met Met Tyr Gly Cys His Pro Pro
580 585 590
Trp Pro Leu Cys Ser Arg Pro Arg Ile Val Arg Ser Thr Tyr Pro Cys
595 600 605
His Thr Ala Tyr Ile Leu His Thr Arg Val Pro Gln Phe Gly Trp Glu
610 615 620
Lys Thr Ile Gln Ser Ser Leu Asn Gly Arg Pro Pro Lys Val Pro Arg
625 630 635 640
Pro Gly Pro Thr Ser Lys Trp Ala Thr Ala Glu Ser Pro Pro Glu Cys
645 650 655
Gln Lys Phe Pro Ala Ala Glu Phe Ala Glu Asp Gly Ile Gln Val Thr
660 665 670
Leu Lys Thr Gly Gly Ala Glu Ser Arg Asp Leu Leu His Ser His Val
675 680 685
Glu Gly Val Thr Ser Cys Ile Pro Asn Leu Leu Val Val Ser Asp Met
690 695 700
Gly Gln Gln Leu Gly Pro Phe Tyr Thr His Asp Val Leu Ala Asp Val
705 710 715 720
Ile His Val Leu Ser Glu Glu Asp Arg Leu Val Tyr Gln Arg Gln Arg
725 730 735
Glu Asp His Phe Tyr Trp Asn Lys Lys Leu Gln Pro Thr Lys Ala Gly
740 745 750
Trp Arg Leu Asp Arg Tyr Lys Phe Leu Ala Met Ile Glu Tyr Ala Tyr
755 760 765
Ala Arg Asn Pro Ser Ala Lys Trp Tyr Val Phe Met Glu Ala Asp Thr
770 775 780
Thr Val Ile Trp Gln Asn Leu Val Gln Leu Leu Ser Arg Tyr Lys Trp
785 790 795 800
Ile Asp Pro Ile Tyr Ile Gly Ser Pro Thr Pro Gly Arg Pro Ile Gly
805 810 815
Thr Trp Trp Asp Pro Glu Pro Thr Phe Phe Val Tyr Gly Gly Ser Gly
820 825 830
Ile Val Leu Ser Val Thr Ala Ile Glu His Leu Leu Arg Glu Asp Leu
835 840 845
Glu Gly Ser Ala Lys Ala Glu Ser Gly Gln Thr Ser Gln Leu Leu Ile
850 855 860
Thr Lys Tyr Gln Asp Met Val Arg Glu Asp Cys Cys Gly Asp Ser Val
865 870 875 880
Leu Gly Trp Val Ala Ala Gln Arg Asp Val Lys Ile Lys Gly Leu Trp
885 890 895
Pro Met Phe Asn Pro His Pro Leu His Gly Thr Pro Leu Gly Lys Ser
900 905 910
Tyr Trp Cys Gln Pro Val Ile Thr Phe His Lys Ser Asp Pro Val Gln
915 920 925
Val Val Glu Leu Trp Lys Trp Gln Thr Glu Arg Gln Lys Lys Gly Asn
930 935 940
Asp Pro Arg Pro Ile Leu Tyr Ser Asp Ile Val Asp Phe Phe Asp Phe
945 950 955 960
Glu Ala Thr Pro Val Arg Glu Asn Trp Asn Asn Ala Asp Met Asp Ser
965 970 975
Phe Asp Ala Ser Thr His Glu Ala His Asp Ser Phe Asp Ser Cys Lys
980 985 990
Glu Ala Cys His Lys His Asp His Cys Phe Gln Phe Thr Tyr His Arg
995 1000 1005
Lys Lys Cys Arg Met Ala Arg Val Ile Arg Leu Gly Asn Ser Val
1010 1015 1020
Ser Met Asp Gly Asp Gly Asp Ala Lys Ala Gly Arg Ser Leu Ala
1025 1030 1035
Gly Trp Asp Val Lys Lys Ile Lys Glu Phe Lys Lys Ala His Asn
1040 1045 1050
Cys Glu Lys Val Asp Trp Pro Glu Pro Ser Thr Glu Arg Ile Phe
1055 1060 1065
<210> 569
<211> 593
<212> PRT
<213> 产黄青霉
<400> 569
Met Arg Leu Gln Phe Leu Leu Leu Arg Ala Leu Gly Leu Phe Leu Cys
1 5 10 15
Tyr Thr Gln Leu Val Phe Ala Val Asp Ala Ser Tyr Glu Leu Val Glu
20 25 30
Gln Leu Asp Lys Ala Pro Asp Gly Trp Ser Arg Gly Asp Lys Pro Pro
35 40 45
Ala Ser Lys Phe Ile Lys Phe Arg Leu Ala Ile Ala Gln His Asn Ala
50 55 60
Arg Glu Phe Glu Gln Lys Val Ile Glu Leu Ser Thr Pro Glu His Pro
65 70 75 80
Ser Tyr Gly Gln His Met Ser Arg Asp Glu Val Lys Gln Phe Leu Thr
85 90 95
Pro His Pro Ala Ile Ser Asp Arg Ile Ile Ala Trp Leu Arg Ser Glu
100 105 110
Asn Val Pro Ala Asn Ser Ile Asn Leu Tyr Gly His Trp Val Ser Phe
115 120 125
Ile Val Pro Ile Phe Gln Ala Glu Arg Met Leu Lys Thr Gln Phe Phe
130 135 140
Tyr Phe His Gln Gln Gly Gln Glu Asn Pro Ala Ile Arg Thr Leu Gly
145 150 155 160
Tyr Ser Val Pro Arg Ala Leu Arg Ser His Ile Gln Leu Ile Gln Pro
165 170 175
Thr Thr Leu Phe Gly Asn Leu Val Pro Gln Lys Ser Pro Val Phe Lys
180 185 190
Gln Lys Ile Ala Thr Pro Glu Lys Leu Ala Ala Gly Cys Asp Val Thr
195 200 205
Ile Thr Pro Asp Cys Leu Arg Asp Leu Tyr Lys Ile Asp Asn Thr Thr
210 215 220
Thr Arg Pro Asp Trp Arg Asn Arg Leu Gly Ile Ser Gly Phe Leu Glu
225 230 235 240
Gln Tyr Ala Arg His Asp Asp Phe Asn Glu Phe Leu Arg Arg Tyr Ala
245 250 255
Gln Ser His Thr Asp Ala Asn Phe Ser Val Val Ser Ile Asn Gly Gly
260 265 270
Gln Asp Gly Gln His Ser Met Ser Asp Ser Val Glu Ala Asn Leu Asp
275 280 285
Val Gln Tyr Ser Ile Pro Leu Ala Asp Glu Val Leu Ala Thr Phe Tyr
290 295 300
Ser Thr Gly Gly Arg Gly Pro Val Val Pro Glu Ile Thr His Pro Asp
305 310 315 320
Pro Thr Glu Ser Ser Asn Glu Pro Tyr Leu Glu Gln Leu His Tyr Leu
325 330 335
Leu Asp Leu Pro Asp Glu Glu Leu Pro Ala Val Leu Ser Asn Ser Tyr
340 345 350
Gly Glu Asn Glu Gln Ser Leu Pro Pro Ser Tyr Leu Asn Ala Thr Cys
355 360 365
Ser Leu Phe Ala Gln Leu Gly Ala Arg Gly Val Ser Ile Leu Phe Gly
370 375 380
Ser Gly Asp Ser Gly Pro Gly Ser Ser Cys Val Lys Asn Asp Gly Thr
385 390 395 400
Asn Gln Thr Arg Phe Leu Pro Gly Tyr Pro Ala Ser Cys Pro Phe Val
405 410 415
Thr Ala Val Gly Gly Thr Tyr Gly Thr Asn Pro Glu Lys Ala Val Pro
420 425 430
Phe Ser Gly Gly Gly Phe Ser Glu Val Phe Ser Arg Pro Gln Tyr Gln
435 440 445
Asp Gln Ala Val Lys Gly Tyr Leu Gly His Leu Gly Asn Gln Trp Asn
450 455 460
Gly Leu Tyr Asn Ala Thr Gly Arg Gly Tyr Pro Asp Ile Ser Ala Gln
465 470 475 480
Ala Ala Asn Phe Ile Val Arg Asp Gln Gly Asp Trp Ile Ser Val Gly
485 490 495
Gly Thr Ser Ala Ser Thr Pro Val Leu Ala Gly Val Ile Ser Arg Leu
500 505 510
Asn Ala Ala Arg Ile Ala Gln Gly Lys Pro Arg Met Gly Phe Leu Asn
515 520 525
Pro Trp Leu Tyr Ser His Gly Gln Thr Gly Phe Thr Asp Ile Val Leu
530 535 540
Gly Ser Ser Ser Gly Cys Lys Trp Thr Ser Asp Ala Arg Val Arg Asn
545 550 555 560
Ala Ser Trp Asp Ala Thr Glu Gly Trp Asp Pro Ala Thr Gly Leu Gly
565 570 575
Thr Pro Leu Phe Pro Ala Leu Val Lys Leu Val Leu Ser Asp Gly Gln
580 585 590
Ala
<210> 570
<211> 568
<212> PRT
<213> 烟曲霉
<400> 570
Met Leu Ser Ser Thr Leu Tyr Ala Gly Trp Leu Leu Ser Leu Ala Ala
1 5 10 15
Pro Ala Leu Cys Val Val Gln Glu Lys Leu Ser Ala Val Pro Ser Gly
20 25 30
Trp Thr Leu Ile Glu Asp Ala Ser Glu Ser Asp Thr Ile Thr Leu Ser
35 40 45
Ile Ala Leu Ala Arg Gln Asn Leu Asp Gln Leu Glu Ser Lys Leu Thr
50 55 60
Thr Leu Ala Thr Pro Gly Asn Pro Glu Tyr Gly Lys Trp Leu Asp Gln
65 70 75 80
Ser Asp Ile Glu Ser Leu Phe Pro Thr Ala Ser Asp Asp Ala Val Leu
85 90 95
Gln Trp Leu Lys Ala Ala Gly Ile Thr Gln Val Ser Arg Gln Gly Ser
100 105 110
Leu Val Asn Phe Ala Thr Thr Val Gly Thr Ala Asn Lys Leu Phe Asp
115 120 125
Thr Lys Phe Ser Tyr Tyr Arg Asn Gly Ala Ser Gln Lys Leu Arg Thr
130 135 140
Thr Gln Tyr Ser Ile Pro Asp His Leu Thr Glu Ser Ile Asp Leu Ile
145 150 155 160
Ala Pro Thr Val Phe Phe Gly Lys Glu Gln Asn Ser Ala Leu Ser Ser
165 170 175
His Ala Val Lys Leu Pro Ala Leu Pro Arg Arg Ala Ala Thr Asn Ser
180 185 190
Ser Cys Ala Asn Leu Ile Thr Pro Asp Cys Leu Val Glu Met Tyr Asn
195 200 205
Leu Gly Asp Tyr Lys Pro Asp Ala Ser Ser Gly Ser Arg Val Gly Phe
210 215 220
Gly Ser Phe Leu Asn Glu Ser Ala Asn Tyr Ala Asp Leu Ala Ala Tyr
225 230 235 240
Glu Gln Leu Phe Asn Ile Pro Pro Gln Asn Phe Ser Val Glu Leu Ile
245 250 255
Asn Arg Gly Val Asn Asp Gln Asn Trp Ala Thr Ala Ser Leu Gly Glu
260 265 270
Ala Asn Leu Asp Val Glu Leu Ile Val Ala Val Ser His Pro Leu Pro
275 280 285
Val Val Glu Phe Ile Thr Gly Ala Leu Pro Pro Val Leu Arg Val Leu
290 295 300
Ala Leu Gln Thr Gln Leu Pro Ser Ser Ser Gly Asp Phe Gln Leu Thr
305 310 315 320
Val Pro Glu Tyr Tyr Ala Arg Arg Val Cys Asn Leu Ile Gly Leu Met
325 330 335
Gly Leu Arg Gly Ile Thr Val Leu Glu Ser Ser Gly Asp Thr Gly Ile
340 345 350
Gly Ser Ala Cys Met Ser Asn Asp Gly Thr Asn Lys Pro Gln Phe Thr
355 360 365
Pro Thr Phe Pro Gly Thr Cys Pro Phe Ile Thr Ala Val Gly Gly Thr
370 375 380
Gln Ser Tyr Ala Pro Glu Val Ala Trp Asp Gly Ser Ser Gly Gly Phe
385 390 395 400
Ser Asn Tyr Phe Ser Arg Pro Trp Tyr Gln Ser Phe Ala Val Asp Asn
405 410 415
Tyr Leu Asn Asn His Ile Thr Lys Asp Thr Lys Lys Tyr Tyr Ser Gln
420 425 430
Tyr Thr Asn Phe Lys Gly Arg Gly Phe Pro Asp Val Ser Ala His Ser
435 440 445
Leu Thr Pro Tyr Tyr Glu Val Val Leu Thr Gly Lys His Tyr Lys Ser
450 455 460
Gly Gly Thr Ser Ala Ala Ser Pro Val Phe Ala Gly Ile Val Gly Leu
465 470 475 480
Leu Asn Asp Ala Arg Leu Arg Ala Gly Lys Ser Thr Leu Gly Phe Leu
485 490 495
Asn Pro Leu Leu Tyr Ser Ile Leu Ala Glu Gly Phe Thr Asp Ile Thr
500 505 510
Ala Gly Ser Ser Ile Gly Cys Asn Gly Ile Asn Pro Gln Thr Gly Lys
515 520 525
Pro Val Pro Gly Gly Gly Ile Ile Pro Tyr Ala His Trp Asn Ala Thr
530 535 540
Ala Gly Trp Asp Pro Val Thr Gly Leu Gly Val Pro Asp Phe Met Lys
545 550 555 560
Leu Lys Glu Leu Val Leu Ser Leu
565
<210> 571
<211> 580
<212> PRT
<213> 烟曲霉
<400> 571
Met Tyr Pro Leu Asp Gly Ser Ala Arg Pro His Pro Pro Gly Thr Thr
1 5 10 15
Arg Leu Asn Ser Val Glu Pro Asp Lys Gln Ile Gly Phe Thr Val Leu
20 25 30
Val Arg Pro Gln Thr Gly Ala Pro Arg Leu Pro Asp Leu Ala Gln Trp
35 40 45
Gln Ala Ile Pro Ile Ala Glu Arg Gln Phe Leu Ser Thr Ala Gly Phe
50 55 60
Glu Arg Thr Tyr Gly Ser Ser Glu Asp Asp Ile Val Ser Val Ala Ser
65 70 75 80
Phe Leu Glu Lys Ala Gly Met Thr Ile Arg Ser Arg His Ala Gly Ala
85 90 95
Gly Thr Val Glu Val Gln Ala Lys Thr Cys Gln Ile His Ser Val Phe
100 105 110
Ala Val Gln Leu Leu Tyr Tyr Arg Gly Gln Leu Arg Pro Ala Ala Arg
115 120 125
Lys Arg Arg Asp Asp Lys Gln Pro Ala Glu Glu Thr Tyr Ile Gly Phe
130 135 140
Glu Gly Cys Ile Ser Leu Pro Ala Ala Leu His Asp Lys Val Ile His
145 150 155 160
Ile Phe Gly Leu Asp Thr Arg Thr Phe Gly Ala Ser Gly Gly Tyr Ser
165 170 175
Gly Asp Pro Pro Arg Ala Gln Arg Leu Ile Val Ala Glu Leu Ala Ala
180 185 190
Leu Tyr Gly Phe Pro Ala Gly Val Asp Ala Ser Gln Gln Thr Ile Gly
195 200 205
Ile Phe Ser Gly Glu Gly Asn Asp Asp Lys Gly Gln Ser Leu Ser Asn
210 215 220
Tyr Arg Pro Ala Asp Val Ala Ala Tyr Phe Asn Asn Gln Thr Val Gly
225 230 235 240
Tyr Asn Arg Ala Pro Thr Val Val Pro Val Ser Leu Thr Val Ala Asp
245 250 255
Gln Thr Tyr Arg Asn Asp Pro Asp His Pro Thr Gln Glu Leu Ser Gln
260 265 270
Asp Ile Met Thr Ala Ala Thr Ile Ala Gln Gly Cys Thr Val Asn Val
275 280 285
Tyr Phe Ser Asp Leu Thr Glu Gln Gly Trp Leu Ala Phe Leu Thr Arg
290 295 300
Val Leu Phe Pro Gln Gly Glu Glu Lys Arg Pro Thr Ile Val Ser Ile
305 310 315 320
Ser Trp Thr Met Tyr Asp Glu Gln Thr Tyr Arg Asp Arg Leu Ser Phe
325 330 335
Leu Phe Gln Arg Leu Ala Val Val Gly Thr Ser Val Phe Ala Ile Ala
340 345 350
Gly Asp Trp Gly Ala Asn Asn Asn Ile Ile Asp Gly Gln Pro His Val
355 360 365
Gly Trp Pro Gly Ser Asp Pro Trp Val Thr Cys Val Gly Gly Thr Val
370 375 380
Val Gly Asn Val Arg Ser Ser Gly Ala Phe Thr Glu His Ala Trp Ser
385 390 395 400
Asp Arg Asp Asn Pro Asp Ser Gln Phe Thr Ile Asp Gly His Leu Gly
405 410 415
Val Thr Gly Gly Gly Met Ser Arg Val Phe Ala Thr Pro Pro Tyr Gln
420 425 430
Leu Ser Ser Gly Ile Ser Ala Val Thr Asp Cys Asn Gly Glu Arg Trp
435 440 445
Thr Gly Gly Arg Phe Ile Pro Asp Ile Thr Gly Met Val Gly Phe Arg
450 455 460
Gly Phe Ile Val Asn Gly Lys Arg Asn Tyr Phe Ile Gly Thr Ser Cys
465 470 475 480
Ser Thr Pro Leu Tyr Ala Gly Leu Phe Ala Ala Leu Ala Ser Ala Leu
485 490 495
Gly Gly Gly Gly Glu Gly Gly Val Leu Phe Gly Pro Leu Asn Thr Val
500 505 510
Leu Tyr Gln Ile Asp Arg Gly Val Tyr Arg Asp Ile Thr Phe Gly His
515 520 525
Asn Asp Ser Gly Asp Met Pro Ala Cys Ala Tyr Phe Ala Ala Gly Glu
530 535 540
Gly Tyr Asp Thr Val Ser Gly Leu Gly Ser Val Asp Gly Arg Arg Thr
545 550 555 560
Leu Glu Glu Leu Arg Arg Ile Tyr Trp Pro Lys Thr Lys Gly Phe Gly
565 570 575
Cys Phe Arg Asn
580
<210> 572
<211> 454
<212> PRT
<213> 粗糙链孢霉
<400> 572
Met Arg Ala Thr Leu Val Val Val Leu Cys His Leu Ser Leu Ala Phe
1 5 10 15
Ala Leu Ala Ile Ser Pro Ala Ala Ser His Trp Lys Arg Ser Ala Arg
20 25 30
Leu Ala Ser Asp Gln Thr Ala Ser Glu Arg Tyr Ser Leu Pro Ser Arg
35 40 45
Val Ala Arg Tyr Ile Asp Tyr Val Leu Pro Ala Pro Asp Pro Asp Pro
50 55 60
Val Ser Ser Ala Pro Lys Ser Val Ala Val Gln Asp Pro Pro Thr Leu
65 70 75 80
Lys Gly Val Ile Gly Ala Arg Gln Thr Arg Asp Val Asp Cys Leu Gln
85 90 95
Tyr Ile Ala Pro Gln Cys Leu Arg Gln Leu Ala Trp Leu Ala Glu Asp
100 105 110
Leu Asp Met Phe Phe Gly Asp Phe Ala Pro Asp Leu Leu Thr Asn Phe
115 120 125
Asn Leu Glu Pro Asn Leu Asp Tyr Lys Tyr Thr Met Ala Met Ala Lys
130 135 140
Pro Ile Pro Val Thr Asn Ile Gln Val Gly Asp Phe Val Val Gln Gly
145 150 155 160
Asn Met Asn Ile Met Leu Ala Ala Phe Asn Ala His Tyr Cys Arg Thr
165 170 175
Gly Leu Asp Pro Gln Phe Asp Pro Val Tyr Pro Asn Pro Ala Pro Gly
180 185 190
Gly Tyr Asn Ala Ser Asp Cys Gly Thr His Val Pro Pro Arg Val Ile
195 200 205
Ala Ile Met Tyr Ala Trp Asn Lys Ala Trp Tyr Ser Asp Ala Asp Phe
210 215 220
Ala Ser Ile Phe Pro Ala Ser Asp Pro Trp Val Thr Ser Val Gly Gly
225 230 235 240
Thr Gln Phe Leu Pro Val Val Ser Asn Gly Ser Ser Ser Thr Thr Ala
245 250 255
Ser Ser Gly Met Pro Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser
260 265 270
Ser Ser Ser Ser Ser Ser Ser Ser Ser Leu Phe Pro Gly Glu Thr Ala
275 280 285
Leu Asp Asp Asn Asn Thr Gly Ser Ser Gly Gly Ser Phe Ser Arg Leu
290 295 300
Phe Pro Gly Pro Trp Tyr Gln Gly Asn Leu Thr Arg Glu Tyr Leu Ala
305 310 315 320
Ser Ala Pro Gly Ala Ala Glu Leu Ala Arg Gln Gly Tyr Phe Asn Gly
325 330 335
Ser Gly Arg Gly Tyr Pro Asp Ile Ser Ala Met Ala Arg Ser Phe Leu
340 345 350
Val Ala Leu His Gly Gly Tyr His Ala Val Ser Gly Thr Ser Ala Ser
355 360 365
Thr Pro Val Val Ala Ala Met Val Ala Lys Ile Asn Asp Ala Arg Leu
370 375 380
His Ala Gly Lys Ser Thr Val Gly Phe Leu Asn Pro Val Leu Tyr Ser
385 390 395 400
Ala Ala Ala Gly Lys Ala Gly Val Leu Arg Asp Val Pro Leu Gly Lys
405 410 415
Asn His Asp Cys Gly Val Gly Glu Ala Phe Pro Ala Arg Arg Ala Trp
420 425 430
Asp Ala Val Thr Gly Leu Gly Thr Pro Asp Phe Glu Lys Leu Lys Glu
435 440 445
Leu Tyr Leu Gly Leu Pro
450
<210> 573
<211> 652
<212> PRT
<213> 米曲霉
<400> 573
Met Arg Pro Leu Ser His Leu Ser Phe Phe Asn Gly Leu Leu Leu Gly
1 5 10 15
Leu Ser Ala Leu Ser Ala Ala Thr Ser Val Val His Glu Arg Arg Glu
20 25 30
Ala Thr Ser Ser Asn Trp Val Lys Arg Ala Arg Val Asn Pro Ser Asp
35 40 45
Lys His Val Val Arg Ile Gly Leu Thr Gln Ser Ser Leu Glu Glu Ala
50 55 60
His Asp Leu Leu Met Asp Val Ser Asn Pro Ser Ser Pro Asn Tyr Ala
65 70 75 80
Arg Phe Tyr Ser Ala Asp Glu Val Ala Ala Lys Phe Ala Pro Ser Thr
85 90 95
Glu Thr Val Asn Glu Val Gln Asn Trp Leu Thr Glu Lys Gly Ile Asn
100 105 110
Ala Ser Arg Val Ala Arg Thr Gln Asn His Gly Trp Leu Val Phe His
115 120 125
Ala Thr Ser Lys Glu Ile Glu Asn Leu Phe Asp Thr Thr Tyr Tyr Glu
130 135 140
Tyr His Asn Arg Lys Thr Gly Lys Lys Ala Ile Ala Cys Glu Gln Tyr
145 150 155 160
His Val Pro Ala Ser Val Gln Lys His Ile Asp Tyr Val His Pro Gly
165 170 175
Val Asn Leu Asn Pro Ser Ser Gly Lys Pro Ser Ser Ile Arg Arg Arg
180 185 190
Ala Ala Ala Ser Lys Lys Thr Lys Leu Pro Ala Arg Gly Pro Arg Pro
195 200 205
Ile Gln Gln His Asp Val Lys Gly Leu Asn Val Thr Asn Cys Asp Gln
210 215 220
Leu Ile Thr Pro Glu Cys Ile Arg Ala Leu Tyr Lys Ile Pro Ser Ala
225 230 235 240
Arg Ala Ala Pro His Pro Asn Asn Ser Leu Gly Ile Phe Glu Glu Gly
245 250 255
Asp Tyr Tyr Ala Gln Glu Asp Leu Asp Leu Phe Phe Lys Thr Phe Ala
260 265 270
Lys Asp Ile Pro Gln Gly Thr His Pro Ile Pro Ala Phe Ile Asp Gly
275 280 285
Ala Glu Ala Pro Val Pro Val Thr Lys Ala Gly Gly Glu Ser Asp Leu
290 295 300
Asp Phe Glu Leu Ala Tyr Pro Ile Val His Pro Gln Ser Ile Thr Leu
305 310 315 320
Tyr Gln Thr Asp Asp Ala Asn Trp Ala Ser Asn Thr Thr Gly Phe Leu
325 330 335
Asn Thr Phe Leu Asp Ala Leu Asp Gly Ser Tyr Cys Thr Tyr Cys Ala
340 345 350
Tyr Gly Glu Cys Gly Asn Asp Pro Ser Leu Asp Pro Val Tyr Pro Asp
355 360 365
Asp Ala Gly Tyr Asp Gly Gln Leu Met Cys Gly Val Phe Lys Pro Thr
370 375 380
Asn Val Ile Ser Val Ser Tyr Gly Glu Gln Glu Asn Asp Leu Pro Ala
385 390 395 400
Asn Tyr Gln Gln Arg Gln Cys Met Glu Phe Leu Lys Leu Gly Leu Gln
405 410 415
Gly Val Ser Val Leu Phe Ala Ser Gly Asp Asn Gly Val Ala Gly Pro
420 425 430
Pro Gly Asp Gly Asn Ser Val Asn Gly Cys Leu Asn Asn Gly Thr Val
435 440 445
Phe Ser Pro Ala Phe Pro Asn Ser Cys Pro Tyr Ile Thr Asn Val Gly
450 455 460
Ala Thr Lys Val Tyr Pro Gly Tyr Thr Val Ser Gln Pro Glu Ser Ala
465 470 475 480
Val Tyr Asp Pro Asp Gly Leu Tyr Ser Tyr Ala Ser Gly Gly Gly Phe
485 490 495
Ser Asn Ile Tyr Pro Ile Pro Asp Tyr Gln Ala Glu Ala Val Ala Thr
500 505 510
Tyr Phe Lys Asp His Asn Pro Pro Tyr Pro Tyr Tyr Glu Gly Ala Glu
515 520 525
Asn Leu Gly Lys Asn Gly Gly Leu Tyr Asn Arg Leu Gly Arg Gly Tyr
530 535 540
Pro Asp Val Ala Ala Asn Gly Asp Asn Ile Ala Val Phe Asn Gly Gly
545 550 555 560
Glu Phe Gly Ser Ser Gly Gly Thr Ser Ala Ser Thr Pro Ile Phe Ala
565 570 575
Ser Ile Ile Asn Arg Ile Ile Asp Glu Arg Leu Ala Val Gly Lys Gly
580 585 590
Pro Val Gly Phe Ile Asn Pro Val Leu Tyr Lys Asn Pro Ser Val Leu
595 600 605
Asn Asp Ile Thr Asn Gly Thr Asn Pro Gly Cys Gly Thr Asp Gly Phe
610 615 620
Ser Thr Ala Pro Gly Trp Asp Pro Ala Thr Gly Leu Gly Thr Pro Asn
625 630 635 640
Tyr Pro Lys Met Leu Lys Leu Trp Leu Asp Leu Pro
645 650
<210> 574
<211> 243
<212> PRT
<213> 嗜热毁丝霉
<400> 574
Met Lys Leu Ala Val Leu Ile Ala Thr Thr Ala Gly Leu Ala Ala Ala
1 5 10 15
Leu Pro Gln Gly Val Ala Arg Arg Gly Val Gly Arg Pro Leu His His
20 25 30
Ser Gly Pro Asn Ile Arg Asn Thr Thr Tyr Pro Gln Tyr Ser Ser Asn
35 40 45
Trp Ala Gly Ala Val Gln Ile Gly Thr Gly Phe Thr Ser Val Tyr Gly
50 55 60
Thr Ile Thr Val Pro Ser Val His Asp Arg Asn Pro Asn Ala Ala Ala
65 70 75 80
Ser Ala Trp Val Gly Ile Asp Gly Asp Thr Cys Gln Gln Ala Ile Leu
85 90 95
Gln Thr Gly Val Ser Phe Tyr Gly Asp Gly Ser Phe Asp Ala Trp Tyr
100 105 110
Glu Trp Ile Pro Asp Tyr Ala Tyr Ser Phe Ser Asn Phe Arg Leu Ser
115 120 125
Ala Gly Asp Gln Ile Arg Met Ser Val Glu Ala Ser Ser Lys Arg Ala
130 135 140
Gly Val Ala Thr Leu Glu Asn Leu Ser Thr Gly Gln Lys Val Ser His
145 150 155 160
Thr Phe Thr Ser Thr Pro Ser Thr Leu Cys Glu Thr Asn Ala Glu Trp
165 170 175
Ile Val Glu Asp Phe Gln Glu Gly Ser Ser Leu Val Pro Phe Ala Asp
180 185 190
Phe Gly Thr Val Thr Phe Thr Asp Ala Tyr Ala Thr Gly Ser Ser Gly
195 200 205
Thr Val Thr Pro Ser Gly Ala Thr Ile Ile Asp Ile Lys Gln Gly Asn
210 215 220
Glu Val Leu Thr Asn Cys Ala Thr Ser Gly Ser Asp Leu Thr Cys Ser
225 230 235 240
Tyr Thr Gly
<210> 575
<211> 256
<212> PRT
<213> 米曲霉
<400> 575
Met Lys Leu Phe Ala Ile Leu Ser Ser Thr Val Leu Ala Ser Val Ala
1 5 10 15
Leu Ala Ser Pro Leu Thr Leu Glu Arg Arg Ala Arg Asn Ala Ala Arg
20 25 30
Leu Gln Ala Arg Val Ala Gln Arg His Ser Asn Leu Pro Phe Lys Ala
35 40 45
Gly Thr Asn Glu Ile Leu His Leu Asn Glu Thr Thr His Glu Glu Tyr
50 55 60
Ser Ser Asn Trp Ala Gly Ala Val Leu Ile Gly Ser Gly Leu His Val
65 70 75 80
Arg Asp Arg Asp Glu Gln Tyr Cys Ala Thr Ala Trp Val Gly Ile Asp
85 90 95
Gly Asp Thr Cys Gln Ser Ala Ile Leu Gln Thr Gly Val Asp Phe Cys
100 105 110
Ile Asp Ser Ser Gly Thr Thr Ser Asp Ala Trp Tyr Glu Trp Phe Pro
115 120 125
Asp Tyr Ser His Asp Phe Ser Gly Ile Ser Ile Ser Ala Gly Asp Lys
130 135 140
Val Lys Val Thr Val Asp Ala Ser Ser Lys Thr Ala Gly Thr Ala Ile
145 150 155 160
Val Glu Asn Leu Thr Thr Gly Lys Thr Val Ser His Thr Phe Ala Gly
165 170 175
Gln Asp Asp Asn Ala Leu Cys Glu Thr Asn Ala Glu Trp Ile Met Glu
180 185 190
Asp Phe Ser Ser Phe Leu Ser Leu Val Pro Phe Ala Asn Phe Gly Thr
195 200 205
Val Thr Phe Thr Asp Ile Ser Ala Thr Ser Gly Asp Ser Ser Val Gly
210 215 220
Ala Ser Asn Ala Thr Ile Ile Asp Ile Gln Gln Asn Asn Lys Thr Leu
225 230 235 240
Thr Ser Ser Ser Ala Ser Asp Thr Glu Val Thr Ile Lys Tyr Ile Gly
245 250 255
<210> 576
<211> 288
<212> PRT
<213> 粗糙链孢霉
<400> 576
Met Lys Leu Leu Ser Pro Ala Ile Ser Leu Leu Gly Val Ile Ser Gln
1 5 10 15
Pro Ile Leu Ala Gln Phe Thr Phe Thr Ser Thr Val Glu His Asn Gly
20 25 30
Val Pro Val Pro Gln Ala Glu Thr Asp Leu Lys Pro Phe Lys Pro Gly
35 40 45
Thr Leu Gly Arg Ile Arg Ser Arg Thr Asp Asp Asp Ser Gly Pro Glu
50 55 60
Ile Gly Thr Thr Thr Leu Arg Arg Val Lys Arg Thr Asn Pro Thr Ala
65 70 75 80
Asn Ser Asn Asn Trp Cys Gly Ser Val Gln Ser Thr Thr Ser Ser Asn
85 90 95
Gln Ile Lys Leu Val His Gly Thr Phe Gln His Pro Thr Cys Thr Gln
100 105 110
Arg Pro Gly Val Thr Gln Tyr Pro Gln Ala Ala Ala Ala Trp Ile Gly
115 120 125
Ile Asp Gly Asp Ser Trp Thr Ser Ala Leu Leu Gln Ala Gly Thr Val
130 135 140
Cys Lys Ile Asn Asn Ser Thr Gly Ile Val Glu Asn Glu Val Trp Trp
145 150 155 160
Gln Trp Val Pro Asn Gly Ala Tyr Thr Ile Thr Asn Ile Pro Val Phe
165 170 175
Ala Gly Asp Trp Phe Asp Ile Thr Ile Asn Thr Thr Ser Ser Thr Ala
180 185 190
Ala Thr Ile Lys Ile Met Ser Asn Arg Gly Tyr Thr Tyr Ser Val Asn
195 200 205
Ala Trp Gln Gly Ala Thr Leu Ala Arg Val Asp Ala Asp Trp Val Val
210 215 220
Glu Arg Pro Tyr Tyr Gly Ser Thr Leu Ala Gly Phe Ala Gln Phe Thr
225 230 235 240
Gln Val Trp Phe Gln Asn Ala Tyr Ala Thr Leu Thr Ser Gly Thr Ser
245 250 255
Ser Leu Gly Ile Thr Gly Ala Lys Gln Tyr Gln Ile Pro Gly Gly Cys
260 265 270
Ala Ser Ala Glu Tyr Asp Asn Ser Lys Leu Tyr Ala Ala Val Ala Ala
275 280 285
<210> 577
<211> 282
<212> PRT
<213> 黑曲霉
<400> 577
Met Lys Phe Ser Thr Ile Leu Thr Gly Ser Leu Phe Ala Thr Ala Ala
1 5 10 15
Leu Ala Ala Pro Leu Thr Glu Lys Arg Arg Ala Arg Lys Glu Ala Arg
20 25 30
Ala Ala Gly Lys Arg His Ser Asn Pro Pro Tyr Ile Pro Gly Ser Asp
35 40 45
Lys Glu Ile Leu Lys Leu Asn Gly Thr Thr Asn Glu Glu Tyr Ser Ser
50 55 60
Asn Trp Ala Gly Ala Val Leu Ile Gly Asp Gly Tyr Thr Lys Val Thr
65 70 75 80
Gly Glu Phe Thr Val Pro Ser Val Ser Ala Gly Ser Ser Gly Ser Ser
85 90 95
Gly Tyr Gly Gly Gly Tyr Gly Tyr Trp Lys Asn Lys Arg Gln Ser Glu
100 105 110
Glu Tyr Cys Ala Ser Ala Trp Val Gly Ile Asp Gly Asp Thr Cys Glu
115 120 125
Thr Ala Ile Leu Gln Thr Gly Val Asp Phe Cys Tyr Glu Asp Gly Gln
130 135 140
Thr Ser Tyr Asp Ala Trp Tyr Glu Trp Tyr Pro Asp Tyr Ala Tyr Asp
145 150 155 160
Phe Ser Asp Ile Thr Ile Ser Glu Gly Asp Ser Ile Lys Val Thr Val
165 170 175
Glu Ala Thr Ser Lys Ser Ser Gly Ser Ala Thr Val Glu Asn Leu Thr
180 185 190
Thr Gly Gln Ser Val Thr His Thr Phe Ser Gly Asn Val Glu Gly Asp
195 200 205
Leu Cys Glu Thr Asn Ala Glu Trp Ile Val Glu Asp Phe Glu Ser Gly
210 215 220
Asp Ser Leu Val Ala Phe Ala Asp Phe Gly Ser Val Thr Phe Thr Asn
225 230 235 240
Ala Glu Ala Thr Ser Gly Gly Ser Thr Val Gly Pro Ser Asp Ala Thr
245 250 255
Val Met Asp Ile Glu Gln Asp Gly Ser Val Leu Thr Glu Thr Ser Val
260 265 270
Ser Gly Asp Ser Val Thr Val Thr Tyr Val
275 280
<210> 578
<211> 269
<212> PRT
<213> 产黄青霉
<400> 578
Met Lys Phe Ala Val Ala Ile Phe Ser Thr Thr Leu Leu Ala Thr Ser
1 5 10 15
Val Ile Ala Ser Pro Leu Ala Ser Gln Arg Glu Ala His Ser Glu Ile
20 25 30
Gln Arg Ser Val Gln Gly Pro Arg Arg Thr Thr Arg Pro Arg Asn Pro
35 40 45
Gly Thr Ser Glu Val Leu Tyr Leu Asn Gln Thr Ser Gln Glu Thr Tyr
50 55 60
Ser Ser Lys Trp Ala Gly Ala Val Leu Ile Gly Thr Gly Tyr Thr Ser
65 70 75 80
Val Ser Gly Glu Ile Thr Val Pro Ile Pro His Leu Pro Asn Gly Ala
85 90 95
Asn Ser Tyr Thr Asn Tyr Cys Ala Ser Ala Trp Val Gly Ile Asp Gly
100 105 110
Asp Thr Cys Ser Thr Ala Ile Leu Gln Ala Gly Ile Asp Phe Cys Ile
115 120 125
Gln Gly Gly Phe Ile Ser Tyr Asn Ala Trp Tyr Glu Trp Tyr Pro Asp
130 135 140
Tyr Ala His Asn Phe Ser Asn Ile Gln Ile Leu Pro Gly Asp Val Ile
145 150 155 160
Lys Met Thr Val Asp Ala Thr Ser Lys Ser Ser Gly Phe Val Thr Ile
165 170 175
Glu Asn Leu Ser Thr Gly Val Ser Val Met His Ile Phe Pro Tyr Ser
180 185 190
Leu Arg Gly Asp Leu Cys Glu Phe Asn Ala Glu Trp Ile Val Glu Asp
195 200 205
Phe Ser Val Asn Asn Ala Leu Ala Pro Phe Ala Asp Phe Gly Thr Val
210 215 220
Val Phe Ser Asn Ala Val Ala Ser Arg Gly Gly Asp Thr Tyr Gly Pro
225 230 235 240
Leu Gly Ala Thr Ile Met Asp Ile Tyr Gln Asp Arg Ile Leu Thr Ser
245 250 255
Ser Ser Val Thr Arg Asp Thr Val Thr Ile Ser Tyr Ile
260 265
<210> 579
<211> 310
<212> PRT
<213> 黑曲霉
<400> 579
Met Gly Gly Arg Asp Val Ala Ile Leu Ser Arg His Phe Ala Val Thr
1 5 10 15
Ser Ser Gln Ser Val Asn Gly Val Val Ser Gly Met Phe Gln His Thr
20 25 30
Val Thr Ser Ser Pro Ser Phe Thr Thr Asn Gln Phe Phe Lys Lys Lys
35 40 45
Phe Thr Ala Ala Ile Ala Thr Ala Ile Phe Ala Ser Val Ala Val Ala
50 55 60
Ala Pro Gln Arg Gly Leu Glu Ala Arg Leu Lys Ala Arg Gly Ser Ser
65 70 75 80
Lys Gly Ser Arg Pro Leu Gln Ala Val Ala Arg Pro Ala Ser Thr Lys
85 90 95
Asn Gln Thr Asn Val Glu Tyr Ser Ser Asn Trp Ser Gly Ala Val Leu
100 105 110
Val Glu Pro Pro Ser Ala Ala Ala Thr Tyr Thr Ala Val Thr Gly Thr
115 120 125
Phe Thr Val Pro Glu Pro Thr Gly Asn Ser Gly Gly Ser Gln Ala Ala
130 135 140
Ser Ala Trp Val Gly Ile Asp Gly Asp Thr Tyr Gly Asn Ala Ile Leu
145 150 155 160
Gln Thr Gly Val Asp Phe Thr Val Thr Asp Gly Glu Ala Ser Phe Asp
165 170 175
Ala Trp Tyr Glu Trp Tyr Pro Asp Tyr Ala Tyr Asp Phe Ser Gly Ile
180 185 190
Asp Ile Ser Ala Gly Asp Glu Ile Val Ala Ile Val Glu Ser Tyr Thr
195 200 205
Ser Thr Thr Gly Ile Ala Ile Ile Glu Asn Lys Ser Thr Gly Gln Lys
210 215 220
Val Ser Lys Glu Leu Ser Ser Ser Ser Ser Leu Gly Gly Gln Asn Ala
225 230 235 240
Glu Trp Ile Val Glu Asp Phe Glu Glu Asn Gly Ser Leu Val Asn Leu
245 250 255
Val Asp Phe Gly Thr Val Thr Phe Thr Gly Ala Val Ala Lys Ala Ala
260 265 270
Gly Gly Glu Ser Val Gly Leu Thr Asp Ala Thr Ile Ile Glu Ile Glu
275 280 285
Glu Asn Gly Gln Val Val Thr Asp Val Thr Ile Asp Ser Asp Ser Glu
290 295 300
Val Thr Ile Thr Tyr Glu
305 310
<210> 580
<211> 264
<212> PRT
<213> 米曲霉
<400> 580
Met Lys Phe Thr Thr Ala Ile Ala Thr Ala Ile Leu Ala Gly Ser Ala
1 5 10 15
Leu Ala Ala Pro Glu Arg Gly Leu Ala Ala Arg Val Lys Ala Arg Ala
20 25 30
Ala Gly Ala Arg Gly Ser Arg Pro Leu Gln Thr Val Gln Lys Pro Ala
35 40 45
Gly Val Thr Asn Lys Thr Asn Val Glu Tyr Ser Ser Asn Trp Ser Gly
50 55 60
Ala Val Leu Glu Asn Pro Pro Ser Ala Ser Ala Thr Tyr Thr Ala Val
65 70 75 80
Thr Gly Thr Phe Thr Val Pro Glu Pro Thr Gly Gly Ser Gly Ser Ser
85 90 95
Ser Ala Ser Ala Trp Val Gly Ile Asp Gly Asp Thr Tyr Ser Glu Ala
100 105 110
Ile Leu Gln Thr Gly Val Asp Phe Thr Val Thr Asn Gly Gln Ala Ser
115 120 125
Phe Asp Ala Trp Tyr Glu Trp Leu Pro Asp Tyr Ala Tyr Asp Phe Thr
130 135 140
Gly Ile Asp Ile Ser Ala Gly Asp Val Ile Val Ala Ile Val Glu Ser
145 150 155 160
Tyr Ser Ser Thr Ser Gly Ile Ala Ile Ile Glu Asn Gln Ser Thr Gly
165 170 175
Gln Lys Val Thr Lys Glu Leu Ser Ser Ser Ser Pro Leu Gly Gly Ile
180 185 190
Asn Ala Glu Trp Ile Val Glu Asp Tyr Glu Glu Asn Gly Ser Leu Val
195 200 205
Asp Leu Val Asp Phe Gly Thr Val Thr Phe Thr Gly Ala Glu Ala Lys
210 215 220
Ala Ala Gly Gly Gln Thr Val Gly Leu Thr Asp Ala Thr Ile Ile Glu
225 230 235 240
Ile Thr Gln Asn Asn Lys Val Val Thr Asp Val Thr Ile Asp Ser Asp
245 250 255
Ser Glu Val Thr Ile Thr Tyr Val
260
<210> 581
<211> 279
<212> PRT
<213> 黑曲霉
<400> 581
Met Lys Phe Thr Asn Tyr Leu Leu Thr Thr Ala Thr Leu Ala Ser Ser
1 5 10 15
Val Leu Ala Ala Pro Ala Pro Arg Thr Gly Leu Glu Asp Arg Leu Arg
20 25 30
Ala Arg Ser Leu Gln Arg Gln Ser His Pro Leu Ala Pro Ile Pro Leu
35 40 45
Asp Thr Ser Thr Lys Glu Asn Ser Arg Leu Leu Glu Ala Asp Glu Asn
50 55 60
Thr Thr His Val Thr Tyr Ser Ser Asn Trp Ala Gly Ala Val Arg Glu
65 70 75 80
Gln Pro Pro Pro Gln Gly Thr Tyr Ser Ala Val Ser Ala Thr Phe Arg
85 90 95
Val Pro Glu Pro Thr Ala Gln Gly Gly Ser Gly Thr Gln Ala Gly Ser
100 105 110
Ala Trp Val Gly Ile Asp Gly Asp Thr Tyr Ser Asn Ala Ile Leu Gln
115 120 125
Thr Gly Val Asp Phe Tyr Val Glu Asn Gly Gln Thr Tyr Asn Asp Ala
130 135 140
Trp Tyr Glu Trp Tyr Pro Asp Tyr Ala Tyr Asp Phe Asp Leu Asp Val
145 150 155 160
Ser Thr Gly Asp Thr Ile Val Ala Lys Val Glu Ala Ile Ser Pro Ser
165 170 175
Gln Gly Val Ala Thr Ile Glu Asn Ile Ser Thr Gly Lys Lys Ala Thr
180 185 190
Gln Thr Ile Arg Ala Pro Ala Ala Thr Ala Thr Leu Ala Gly Gln Asn
195 200 205
Ala Asp Trp Ile Val Glu Asp Phe Gln Ser Gly Asp Ser Met Val Asp
210 215 220
Leu Ala Gly Phe Gly Glu Ile Ser Phe Trp Gly Val Gln Ala Gln Gly
225 230 235 240
Gly Gly Ser Thr Trp Gly Val Asp Asp Ala Thr Ile Val Glu Leu Lys
245 250 255
Gln Gly Asn Glu Val Leu Thr Asp Val Glu Val Gln Ser Asp Ser Ala
260 265 270
Phe Thr Val Lys Tyr Thr Ser
275
<210> 582
<211> 279
<212> PRT
<213> 构巢曲霉
<400> 582
Met Lys Leu Ser Leu Thr Ser Ser Leu Leu Thr Thr Thr Thr Leu Leu
1 5 10 15
Gly Ser Ala Leu Ser Leu Pro Gln Thr Ser Asn Leu Val Ser Arg Ile
20 25 30
Gln Ala Arg Ser Leu Asn Arg Gln Ser His Pro Leu Thr Arg Gln Asp
35 40 45
Gly Gly His Ser Gly Ser Gln Thr Gly Phe Arg Met Ala Ser Thr Ala
50 55 60
Ala Val Ala Tyr Ser Asn Asn Trp Ala Gly Val Val Arg Glu Glu Ala
65 70 75 80
Pro Pro Gly Gly Pro Tyr Thr Ala Val Ser Ala Thr Phe Thr Val Pro
85 90 95
Lys Pro Thr Ala Ala Pro Asn Thr Ala Gly Leu Gln Ala Gly Ser Ala
100 105 110
Trp Val Gly Ile Asp Gly Asp Thr Tyr Ser Gly Ala Ile Leu Gln Thr
115 120 125
Gly Val Asp Phe Tyr Ile Asp Asn Gly Arg Val Tyr Asn Asp Ala Trp
130 135 140
Phe Glu Trp Phe Pro Asp Tyr Ala Tyr Asp Phe Asn Leu Ala Ile Asn
145 150 155 160
Thr Gly Asp Val Ile Val Ala Lys Val Glu Ala Leu Ser Pro Ser Asn
165 170 175
Gly Val Ala Ile Ile Glu Asn Lys Asn Thr Gly Gln Thr Ala Thr Gln
180 185 190
Thr Ile Ser Ala Pro Lys Ala Asp Ala Thr Leu Lys Gly Val Asn Ala
195 200 205
Asp Trp Ile Val Glu Asp Phe Gln Ser Gly Asp Lys Val Val Ala Leu
210 215 220
Ala Asp Phe Glu Gln Val Thr Phe Thr Gly Cys Glu Ala Lys Ala Gln
225 230 235 240
Asn Gly Asp Ser Leu Gly Leu Asp Gly Ser Thr Ile Ile Glu Leu Lys
245 250 255
Gln Asn Asn Lys Val Leu Thr Glu Val Thr Val Lys Gly Asn Asn Glu
260 265 270
Leu Thr Val Ala Ser Lys Met
275
<210> 583
<211> 271
<212> PRT
<213> 米曲霉
<400> 583
Met Lys Pro Thr His Ala Tyr Leu Thr Ala Ala Leu Ile Ser Gly Ala
1 5 10 15
Leu Ala Ala Pro Gly Ser Thr Leu Ala Gln Arg Met Arg His Arg Ser
20 25 30
Leu Asp His Leu Thr Leu Pro Leu Gln Gly Glu Gly Ser Ala Thr Asp
35 40 45
Ser Glu Ala Arg Val Ala Gly Ala Ala Val Gln Tyr Ser Asn Asn Trp
50 55 60
Ala Gly Val Val Arg Glu Gln Ala Pro Pro Glu Gly Pro Tyr Thr Ala
65 70 75 80
Val Ser Ala Thr Phe Thr Val Pro Ser Ala Thr Ala Val Ala Asn Gln
85 90 95
Asn Gly Val Gln Ala Gly Ser Val Trp Val Gly Ile Asp Gly Asp Thr
100 105 110
Tyr Ala Gly Ala Ile Leu Gln Ala Gly Val Asp Phe Tyr Ser Asp Pro
115 120 125
Thr Gln Gln Asn His Ala Trp Phe Glu Trp Tyr Pro Ala Tyr Ala Thr
130 135 140
Asn Phe Pro Asn Ile Glu Val Asn Ala Gly Asp Thr Ile Val Ser Thr
145 150 155 160
Val Arg Ser Asn Ser Pro Ser Glu Gly Ile Ala Ile Ile Glu Asn Lys
165 170 175
Ser Thr Gly Gln Thr Val Ser Gln Thr Val Thr Ala Pro Glu Pro Thr
180 185 190
Ala Thr Leu Ala Gly Gln Asn Ala Glu Trp Ile Val Glu Asp Phe Gln
195 200 205
Ser Gly Asp Thr Met Val Val Leu Ala Asn Phe Gly Glu Val Glu Phe
210 215 220
Ser Gly Ala Gln Ala Glu Ala Gly Asn Ala Lys Phe Gly Leu Lys Gly
225 230 235 240
Gly Glu Val Ile Glu Leu Lys Gln Asn Asn Lys Val Leu Thr Gln Thr
245 250 255
Glu Val Thr Gly Asp Gln Asp Met Thr Val Lys Phe Ile Gly Ala
260 265 270
<210> 584
<211> 270
<212> PRT
<213> 产黄青霉
<400> 584
Met Lys Phe Thr Ser Thr Thr Ile Leu Ala Thr Ala Ile Leu Ala Gly
1 5 10 15
Thr Ser Phe Ala Ala Pro Arg Ser Gly Leu Ile Asp Arg Leu Gln Ser
20 25 30
Arg Gly Thr Leu Pro Arg Gln Ser Leu Pro Asp Glu Glu Lys Gly Val
35 40 45
Leu Leu Lys Glu Ala Ala Ala Gly Ala Asn Ala Gln Tyr Ser Lys Asn
50 55 60
Trp Ala Gly Val Val Arg Glu Gln Pro Pro Pro Ser Gly Thr Tyr Ser
65 70 75 80
Ala Val Ser Ala Thr Phe Thr Val Pro Glu Pro Thr Ala Thr Asn Asp
85 90 95
Ser Gly Glu Met Gln Ala Val Ser Ala Trp Val Gly Ile Asp Gly Asp
100 105 110
Thr Tyr Thr Lys Ala Ile Leu Gln Thr Gly Ile Asp Thr Tyr Ile Gln
115 120 125
Asp Gly Lys Lys Thr Phe Asp Ala Trp Tyr Glu Trp Tyr Pro Leu Ser
130 135 140
Ala Glu Asn Phe Asp Leu Ala Leu Ser Ala Gly Asp Val Ile Val Ala
145 150 155 160
Lys Val Glu Thr Tyr Ser Pro Ser Lys Gly Ala Ala Ile Ile Glu Asn
165 170 175
Lys Ser Ser Gly Gln Ser Val Thr Lys Thr Leu Ser Ala Pro Ser Ala
180 185 190
Asp Ala Thr Leu Ala Gly Gln Asn Ala Glu Trp Ile Val Glu Asp Phe
195 200 205
Asn Thr Gly Asn Ser Met Val Pro Leu Val Asn Phe Asn Arg Val Asp
210 215 220
Phe Thr Gly Ala Gln Ala Glu Ala Gly Gly Ala Lys Tyr Gly Val Asn
225 230 235 240
Asn Ala Ala Val Leu Asp Ile Gln Gln Asn Gly Asp Val Lys Ala His
245 250 255
Val Glu Ile Gln Ser Asp Thr Glu Phe Phe Val Thr Tyr Gln
260 265 270
<210> 585
<211> 285
<212> PRT
<213> 嗜热毁丝霉
<400> 585
Met Trp Ser Ile Val Arg Ser Leu Ser Leu Ala Ser Leu Ile Ser Ser
1 5 10 15
Ala Cys Thr Val Thr Ala Gln Leu Ser Phe Val Ala Ser Val Lys Gln
20 25 30
His Gly Lys Asp Val Asp Ala Ser Gly Leu Ser Phe Val Arg Ile Pro
35 40 45
Pro Leu Glu His Arg Trp His Ala Ser Arg Pro Arg Arg Gly Gln Asn
50 55 60
Asn Arg Thr Val Glu Arg Asp Ala Val Ser Tyr Ser Ala Asn Trp Cys
65 70 75 80
Gly Ala Ser Gln His Ala Ser Asp Ser Asp Gly Ile Lys Ser Val Leu
85 90 95
Gly Tyr Phe Thr Ala Pro Asp Leu Thr Leu Arg Pro Gly Thr Pro Ala
100 105 110
Pro Gln Phe Ala Ala Ala Trp Val Gly Ile Asp Gly Ala Ala Cys Asn
115 120 125
Thr Thr Leu Leu Gln Ala Gly Val Thr Thr Ile Val Asn Ser Asp Gly
130 135 140
Gly Gln Ser Ala Ser Ala Trp Trp Glu Trp Tyr Pro Glu Ala Ser Tyr
145 150 155 160
Thr Ile Ser Gly Leu Lys Val Lys Ala Gly Glu Trp Met Ser Val Asn
165 170 175
Ile Thr Thr Lys Asp Ala Ser Ser Ala Ile Leu Val Ile Glu Asn Ala
180 185 190
Asp Thr Gly Thr Ser Val Thr Leu Glu Leu Asn Asn Gly Pro Gln Leu
195 200 205
Cys Arg Arg Asp Ala Glu Trp Ile Leu Glu Asp Phe Tyr Glu Ser Gly
210 215 220
Lys Gln Val Ala Leu Ala Asn Phe Ala Asp Leu Trp Phe Val Asp Ser
225 230 235 240
Gly Ala Thr Thr Val Gly Gly Lys Asn Val Gly Phe Asp Gly Ala Thr
245 250 255
Met Val His Leu Arg Asp Glu Asn Gly Asn Val Leu Cys Ser Pro Glu
260 265 270
Pro Tyr Asp Asn Ser Asn Phe Val Val Val Ser Lys Pro
275 280 285
<210> 586
<211> 293
<212> PRT
<213> 米曲霉
<400> 586
Met Lys Phe Pro Ser Ser Leu Ala Leu Thr Ala Leu Leu Thr Gly Thr
1 5 10 15
Ala Leu Ala Gly Pro His Gly Gln Gly Leu Arg Ala Arg Arg Phe Gln
20 25 30
Gly Pro Gly Trp Leu Gly Glu Ile Asp Leu Asp Ser Pro Ser Glu Ala
35 40 45
Gln Ala Glu Gln Pro Asn Ala Gln Val Thr Pro Ala Pro Gln Ala Lys
50 55 60
Ser Lys Ala Ile Ala His Ser Gln Ala Gln Ser Glu Asp Asp Tyr Leu
65 70 75 80
Ile Asn Glu Asn Trp Ala Gly Ala Val Gln Thr Thr Ala Pro Ala Ala
85 90 95
Ser Ala Thr Tyr Ser Tyr Val Ala Ala Thr Phe Thr Leu Pro Ser Val
100 105 110
Thr Pro Thr Ala Ala Ser Ser Ser Gln Thr Gln Ala Ala Ser Phe Trp
115 120 125
Val Gly Ile Asp Gly Ala Thr Ser Gly Asp Ala Ile Trp Gln Ala Gly
130 135 140
Val Asp Ile Tyr Val Gln Asn Gly Lys Pro Ser Phe Ala Gly Trp Tyr
145 150 155 160
Glu Trp Tyr Pro Ala Asn Ser Val Asn Ile Asp Leu Glu Phe Asn Phe
165 170 175
Gly Asp Val Val Phe Thr Ser Val Glu Ser Thr Ser Asn Ser Glu Gly
180 185 190
Val Ala Val Ile Glu Asn Leu Thr Thr Gly Lys Asn Val Thr Val Thr
195 200 205
Ala Ser Ala Pro Ala Thr Ala Thr Leu Thr Gly Gln Asn Ala Glu Trp
210 215 220
Ile Leu Glu Asp Leu Ala Val Asp Gly Asp Gly Leu Thr Phe Val Asp
225 230 235 240
Phe Gly Glu Ala Thr Phe Thr Gly Cys Val Ala Lys Ala Gly Gly Lys
245 250 255
Glu Tyr Gly Leu Gly Gly Ser Ala Leu Tyr Ala Val Gln Asp Arg Ser
260 265 270
Ser Asn Thr Val Gln Ala Val Pro Thr Ile Val Ser Asp Ser Glu Leu
275 280 285
Lys Val Thr Tyr Gln
290
<210> 587
<211> 307
<212> PRT
<213> 嗜热毁丝霉
<400> 587
Met Lys Pro Thr Val Leu Phe Thr Leu Leu Ala Ser Gly Ala Tyr Ala
1 5 10 15
Ala Ala Thr Pro Ala Ile Pro Gly Tyr Ser Pro Arg Thr Arg Gly Met
20 25 30
Asn Pro His His His Ala Pro Leu Arg Leu Leu His Thr Phe Thr Pro
35 40 45
Ile Ser Thr Ser Gly Lys Ser Phe Arg Leu Leu Ala Ser Ser Thr Glu
50 55 60
Ser Thr Lys Gly Gly Ala Ile Leu Gly Leu Pro Asp Asn Asp Leu Ser
65 70 75 80
Thr Val Arg Thr Thr Ile Arg Ile Pro Ala Ala Lys Met Pro Thr Ala
85 90 95
Gly Pro Thr Ala Asn Asn Thr Val Gly Glu Tyr Ala Ala Ser Phe Trp
100 105 110
Val Gly Ile Asp Ser Ala Thr Asp Ala Cys Gly Ala Gly Gly Ser Leu
115 120 125
Arg Ala Gly Val Asp Ile Phe Trp Asp Gly Thr Leu Gly Gly Gln Gln
130 135 140
Thr Pro Phe Ala Trp Tyr Gln Gly Pro Gly Gln Ala Asp Val Val Gly
145 150 155 160
Phe Gly Gly Gly Phe Pro Val Gly Glu Gly Asp Leu Val Arg Leu Thr
165 170 175
Leu Glu Ala Gly Pro Ala Gly Gly Glu Glu Ile Ala Val Val Ala Glu
180 185 190
Asn Phe Gly Arg Asn Val Thr Arg Ala Asp Glu Gly Ala Val Pro Val
195 200 205
Arg Lys Val Arg Lys Val Leu Pro Ala Glu Ala Gly Gly Gln Lys Leu
210 215 220
Cys Arg Gly Glu Ala Ala Trp Met Val Glu Asp Phe Pro Leu Gln Gly
225 230 235 240
Arg Pro Glu Phe Pro Thr Ala Leu Ala Asn Phe Thr Ser Val Thr Phe
245 250 255
Asn Thr Gly Ile Thr Leu Asp Asp Gly Thr Glu Lys Asp Leu Thr Gly
260 265 270
Ala Glu Val Leu Asp Ile Gln Leu Glu Ala Gln Gly Gly Arg Leu Thr
275 280 285
Ser Cys Glu Val Val Asp Asp Arg Asn Val Lys Cys Ala Arg Val Val
290 295 300
Gly Asp Asn
305
<210> 588
<211> 197
<212> PRT
<213> 嗜热毁丝霉
<400> 588
Met Arg Trp Pro Leu Ala Ala Leu Leu Gly Ser Ala Leu Val Ala Arg
1 5 10 15
Gln Ala Leu Ala Glu Leu Thr Phe Thr Val Glu Ala Thr Arg Asn Gly
20 25 30
Val Pro Ile Pro Ala Ser Glu Ile Arg Leu Glu Pro Phe Glu Pro Gly
35 40 45
Arg Thr Arg Met Gly Ala Val Ala Glu Ala Pro Arg Ala Gln Arg Lys
50 55 60
Thr Arg Arg Ser Asn Ala Gln Ala Asp Ser Ala Asn Trp Cys Gly Ser
65 70 75 80
Val Asn Met Ala Pro Thr Gly Thr Asn Ile Gln Leu Ala His Gly Ser
85 90 95
Phe Gln His Pro Ser Cys Ser Ile Arg Pro Gly Tyr Thr Phe Pro Gln
100 105 110
Ala Ala Ala Ser Trp Val Gly Ile Asp Gly Asp Ser Tyr Arg Asp Ala
115 120 125
Leu Leu Gln Ala Gly Thr Val Cys Lys Ile Asp Asn Ser Thr Gly Val
130 135 140
Val Arg His Glu Ala Trp Trp Gln Trp Val Pro Ser Ala Ala Phe Thr
145 150 155 160
Ile Thr Ser Met Pro Gly Gln Ser Asn Thr Thr Gly Phe Cys Ile Pro
165 170 175
Tyr Ser Ala Pro Phe Val Ser Leu Cys Phe Phe Gly Arg Thr Arg Thr
180 185 190
Cys Leu Phe Leu His
195
<210> 589
<211> 58
<212> PRT
<213> 智人
<400> 589
Met Arg Phe Arg Ile Tyr Lys Arg Lys Val Leu Ile Leu Thr Leu Val
1 5 10 15
Val Ala Ala Cys Gly Phe Val Leu Trp Ser Ser Asn Gly Arg Gln Arg
20 25 30
Lys Asn Glu Ala Leu Ala Pro Pro Leu Leu Asp Ala Glu Pro Ala Arg
35 40 45
Gly Ala Gly Gly Arg Gly Gly Asp His Pro
50 55
<210> 590
<211> 51
<212> PRT
<213> Trichoderma atraviride
<400> 590
Met Ala Ser Thr Asn Ala Arg Tyr Val Arg Tyr Leu Leu Ile Ala Phe
1 5 10 15
Phe Thr Ile Leu Val Phe Tyr Phe Val Ser Asn Ser Lys Tyr Glu Gly
20 25 30
Val Asp Leu Asn Lys Gly Thr Phe Thr Ala Pro Asp Ser Thr Lys Thr
35 40 45
Thr Pro Lys
50
<210> 591
<211> 52
<212> PRT
<213> 里氏木霉
<400> 591
Met Ala Ile Ala Arg Pro Val Arg Ala Leu Gly Gly Leu Ala Ala Ile
1 5 10 15
Leu Trp Cys Phe Phe Leu Tyr Gln Leu Leu Arg Pro Ser Ser Ser Tyr
20 25 30
Asn Ser Pro Gly Asp Arg Tyr Ile Asn Phe Glu Arg Asp Pro Asn Leu
35 40 45
Asp Pro Thr Gly
50
<210> 592
<211> 33
<212> PRT
<213> 里氏木霉
<400> 592
Met Leu Asn Pro Arg Arg Ala Leu Ile Ala Ala Ala Phe Ile Leu Thr
1 5 10 15
Val Phe Phe Leu Ile Ser Arg Ser His Asn Ser Glu Ser Ala Ser Thr
20 25 30
Ser
<210> 593
<211> 84
<212> PRT
<213> 里氏木霉
<400> 593
Met Met Pro Arg His His Ser Ser Gly Phe Ser Asn Gly Tyr Pro Arg
1 5 10 15
Ala Asp Thr Phe Glu Ile Ser Pro His Arg Phe Gln Pro Arg Ala Thr
20 25 30
Leu Pro Pro His Arg Lys Arg Lys Arg Thr Ala Ile Arg Val Gly Ile
35 40 45
Ala Val Val Val Ile Leu Val Leu Val Leu Trp Phe Gly Gln Pro Arg
50 55 60
Ser Val Ala Ser Leu Ile Ser Leu Gly Ile Leu Ser Gly Tyr Asp Asp
65 70 75 80
Leu Lys Leu Glu
<210> 594
<211> 55
<212> PRT
<213> 里氏木霉
<400> 594
Met Leu Leu Pro Lys Gly Gly Leu Asp Trp Arg Ser Ala Arg Ala Gln
1 5 10 15
Ile Pro Pro Thr Arg Ala Leu Trp Asn Ala Val Thr Arg Thr Arg Phe
20 25 30
Ile Leu Leu Val Gly Ile Thr Gly Leu Ile Leu Leu Leu Trp Arg Gly
35 40 45
Val Ser Thr Ser Ala Ser Glu
50 55
<210> 595
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 595
gattaagttg ggtaacgcca gggttttccc agtcacgacg gtttaaacat cgtgcttggg 60
ctattctg 68
<210> 596
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 596
gcgctggcaa cgagagcaga gcagcagtag tcgatgctag gcggccgcgg aaagacgcca 60
gaaagaaa 68
<210> 597
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 597
gtacacttgt ttagaggtaa tccttctttc tagaaggaga gcggccgccg ctcgatgtgg 60
atgatact 68
<210> 598
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 598
atctatactg tctgcaccaa aagtacaaca acgcaaaccg ggcgcgccgg aaagacgcca 60
gaaagaaa 68
<210> 599
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 599
cggtttgcgt tgttgtactt 20
<210> 600
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 600
tggaattgtg agcggataac aatttcacac aggaaacagc gtttaaacac aacccaacgt 60
tctctcgt 68
<210> 601
<211> 69
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 601
gattaagttg ggtaacgcca gggttttccc agtcacgacg gtttaaaccg gttgtccatt 60
tcatccttc 69
<210> 602
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 602
gcgctggcaa cgagagcaga gcagcagtag tcgatgctag gcggccgcgg ggaagcaagt 60
ttcgaagt 68
<210> 603
<211> 66
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 603
gtacacttgt ttagaggtaa tccttctttc tagaaggaga gcggccgcct ccacgctctt 60
ggccac 66
<210> 604
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 604
gtcattaagt ccatcattcc acgtccttca gaccgaattc ggcgcgccgg ggaagcaagt 60
ttcgaagt 68
<210> 605
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 605
atgatgcctt tgcagaaatg gcttgctcgc tgactgatac ggcgcgccta tcgcgaaagt 60
agccaata 68
<210> 606
<211> 58
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 606
agcggataac aatttcacac aggaaacagc gtttaaacca tccttttcct caccacga 58
<210> 607
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 607
tgattgtacc ccagctgcga ttgatgtgta tctttgcatg atttaaattc tccttagctc 60
tgtacagt 68
<210> 608
<211> 28
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 608
gcggccgctc tccttctaga aagaagga 28
<210> 609
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 609
gaattcggtc tgaaggacgt 20
<210> 610
<211> 60
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 610
tgatggtgat gaggcggaaa agcagaggtt acggcgctga ggctttcgtg accgggcttc 60
<210> 611
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 611
gtatcagtca gcgagcaagc 20
<210> 612
<211> 18
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 612
ggaatcgtca ccaaggag 18
<210> 613
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 613
cttctggtga cattccgac 19
<210> 614
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 614
cctctgcgtt gagcaacata 20
<210> 615
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 615
cgaaagcgtg gagtcttctc 20
<210> 616
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 616
aacaaagcct tcacaggcc 19
<210> 617
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 617
tgaggctcct tccaactttt 20
<210> 618
<211> 59
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 618
ggtaacgcca gggttttccc agtcacgacg gtttaaaccg acagcacgtt gtgtgctcc 59
<210> 619
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 619
gcgctggcaa cgagagcaga gcagcagtag tcgatgctag gcggccgctg gagacccagc 60
agccagca 68
<210> 620
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 620
cccgtcaccg agatctgatc cgtcaccggg atccacttaa gcggccgctc agagggaggc 60
tgcccaac 68
<210> 621
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 621
gagactcgaa caaagacatc tttgcgacct cgtccacggc ggcgcgcctg gagacccagc 60
agccagca 68
<210> 622
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 622
gccgtggacg aggtcgcaaa 20
<210> 623
<211> 58
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 623
agcggataac aatttcacac aggaaacagc gtttaaaccc ctgcgccctc ttctgcac 58
<210> 624
<211> 18
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 624
ggaatcgtca ccaaggag 18
<210> 625
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 625
cttctggtga cattccgac 19
<210> 626
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 626
agcagtccac ctgctcaaaa 20
<210> 627
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 627
gattcacacc aatgagtcgg 20
<210> 628
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 628
ccccgacttt gccccgtcac 20
<210> 629
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 629
tcgtcagagt cgtcgcccgt 20
<210> 630
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 630
gcgcagctaa tgtcctctgt 20
<210> 631
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 631
ttgttgagcc agagtcgaga 20
<210> 632
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 632
gtaacgccag ggttttccca gtcacgacgg tttaaacatg agcgtgatcg acaagtg 57
<210> 633
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 633
gcgctggcaa cgagagcaga gcagcagtag tcgatgctag gcggccgccc tctgaggtcg 60
agatggag 68
<210> 634
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 634
tgattgtacc ccagctgcga ttgatgtgta tctttgcatg gcggccgcac gactaatatc 60
cactgccg 68
<210> 635
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 635
aaccaaagtg tacaatgctc atctcgtatt cacatgcaaa ggcgcgcccc tctgaggtcg 60
agatggag 68
<210> 636
<211> 22
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 636
tttgcatgtg aatacgagat ga 22
<210> 637
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 637
gcggataaca atttcacaca ggaaacagcg tttaaactgc tcgatcctac tccaagg 57
<210> 638
<211> 18
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 638
ctgtaaaggc agcatcgg 18
<210> 639
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 639
tcagaacggc ttcaatcatt 20
<210> 640
<211> 18
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 640
ctgtaaaggc agcatcgg 18
<210> 641
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 641
ttacgactcg atccctgtcc 20
<210> 642
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 642
gccgctagga tcgtgataag 20
<210> 643
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 643
gtgtcccagg acgacaactt 20
<210> 644
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 644
tgaaggttgc agtgatctcg 20
<210> 645
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 645
gtaacgccag ggttttccca gtcacgacgg tttaaaccgc attacgaatg cacaaag 57
<210> 646
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 646
gcgctggcaa cgagagcaga gcagcagtag tcgatgctag gcggccgccc atgtcagctc 60
agaccaat 68
<210> 647
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 647
gtacacttgt ttagaggtaa tccttctttc tagaaggaga gcggccgcag gccctggact 60
gctagttt 68
<210> 648
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 648
cgagccatcc gccgcggccc tatattccac ccgagtcctc ggcgcgcccc atgtcagctc 60
agaccaat 68
<210> 649
<211> 22
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 649
gaggactcgg gtggaatata gg 22
<210> 650
<211> 59
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 650
gcggataaca atttcacaca ggaaacagcg tttaaacttg gtcttgaatg aaaggtgtg 59
<210> 651
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 651
gtaacgccag ggttttccca gtcacgacgg tttaaacagg tttgggttgt gagatcg 57
<210> 652
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 652
gcgctggcaa cgagagcaga gcagcagtag tcgatgctag gcggccgcgc gcaaagctac 60
tgggctat 68
<210> 653
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 653
tgattgtacc ccagctgcga ttgatgtgta tctttgcatg gcggccgctc tgctctgctc 60
tgttctgc 68
<210> 654
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 654
aaagttcgtc aaagagcact catagggctg agaaaagcca ggcgcgccgc gcaaagctac 60
tgggctat 68
<210> 655
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 655
tggcttttct cagccctatg 20
<210> 656
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 656
gcggataaca atttcacaca ggaaacagcg tttaaaccaa tgtgtgcctg tttttcg 57
<210> 657
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 657
gtaacgccag ggttttccca gtcacgacgg tttaaacagg tttgggttgt gagatcg 57
<210> 658
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 658
gcgctggcaa cgagagcaga gcagcagtag tcgatgctag gcggccgcgc gcaaagctac 60
tgggctat 68
<210> 659
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 659
caaccagccg cagcctcagc ctctctcagc ctcatcagcc gcggccgctg gcttttctca 60
gccctatg 68
<210> 660
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 660
gcggataaca atttcacaca ggaaacagcg tttaaaccaa tgtgtgcctg tttttcg 57
<210> 661
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 661
tttatccgct tccacgacac 20
<210> 662
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 662
tcttgagcac gacaatcgac 20
<210> 663
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 663
gcatggttgc ctagtgaatg 20
<210> 664
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 664
cgatggtgaa gtcaatgtgg 20
<210> 665
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 665
ggcgattact tccaggacaa 20
<210> 666
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 666
caatggcaat ctggttgttg 20
<210> 667
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 667
gtaacgccag ggttttccca gtcacgacgg tttaaacatg agcgtgatcg acaagtg 57
<210> 668
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 668
gcgctggcaa cgagagcaga gcagcagtag tcgatgctag gcggccgccc tctgaggtcg 60
agatggag 68
<210> 669
<211> 70
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 669
caaccagccg cagcctcagc ctctctcagc ctcatcagcc gcggccgctt tgcatgtgaa 60
tacgagatga 70
<210> 670
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 670
gcggataaca atttcacaca ggaaacagcg tttaaactgc tcgatcctac tccaagg 57
<210> 671
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 671
ttacgactcg atccctgtcc 20
<210> 672
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 672
tcttgagcac gacaatcgac 20
<210> 673
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 673
gcatggttgc ctagtgaatg 20
<210> 674
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 674
gccgctagga tcgtgataag 20
<210> 675
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 675
gtgtcccagg acgacaactt 20
<210> 676
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 676
tgaaggttgc agtgatctcg 20
<210> 677
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 677
ttggtttgaa cagctgcaag 20
<210> 678
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 678
tcttgagcac gacaatcgac 20
<210> 679
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 679
gcatggttgc ctagtgaatg 20
<210> 680
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 680
atggtcagcc agaacctgac 20
<210> 681
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 681
tcttgagccg tttctcgaat 20
<210> 682
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 682
ccgctcttag atcgatggtc 20
<210> 683
<211> 60
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 683
tctccactcg acctgcaggc atgcgcgatc gcgcggccga attcggtctg aaggacgtgg 60
<210> 684
<211> 61
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 684
tggcgcccca cagcacaagc cctgcagact gcttcttcag catgttgaga gaagttgttg 60
g 61
<210> 685
<211> 62
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 685
catctccact cgacctgcag gcatgcgcga tcgcgcggcc gaattcggtc tgaaggacgt 60
gg 62
<210> 686
<211> 60
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 686
ggggatgccc tgtcgagcca gcgggggagg gtgcccaccg ccgcccctcc cgcccagccg 60
<210> 687
<211> 73
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 687
gaaggtggtt tttgcctgta taagccagcc atccggcata ctgcccgcgg ccgccctgca 60
gtgcaggatc tgc 73
<210> 688
<211> 64
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 688
tggcgccccc accgacgtgg gagggctatg atcccagctg gaattagcat atgattaatt 60
aagg 64
<210> 689
<211> 62
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 689
ggtttttgcc tgtataagcc agccatccgg catactgccc gcggccgcag tgcaggatct 60
gc 62
<210> 690
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 690
gatgttgcgc ctgggttgac 20
<210> 691
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 691
cttcttattg atttgagcc 19
<210> 692
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 692
gattgtcatg gtgtacgtga 20
<210> 693
<211> 16
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 693
gagccgcatc gcatag 16
<210> 694
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 694
gcgtcactca tcaaaactgc 20
<210> 695
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 695
cttcggcttc gatgtttca 19
<210> 696
<211> 26
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 696
atggcgtcaa caaatgcgcg ctatgt 26
<210> 697
<211> 75
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 697
attcacactc tcagaataaa ttcatcgcca atttgacagg ccggccattc tcacggtgaa 60
tgtaggcctt ttgta 75
<210> 698
<211> 75
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 698
ttagtagata gcgcacatag cgcgcatttg ttgacgccat gcggttgact attgggtttc 60
tgtgcctcaa aagat 75
<210> 699
<211> 71
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 699
cacactctca gaataaattc atcgccaatt tgacaggccg gccgcccttg tatctctaca 60
cacaggctca a 71
<210> 700
<211> 25
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 700
ctgcagtgca ggatctgcat atatg 25
<210> 701
<211> 70
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 701
ttattcacac tctcagaata aattcatcgc caatttgaca ggccggccac tagtggtctg 60
aaggacgtgg 70
<210> 702
<211> 63
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 702
ttagtagata gcgcacatag cgcgcatttg ttgacgccat ctgatgtctg ctcaagcggg 60
gta 63
<210> 703
<211> 71
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 703
aggaccttaa ttaatcatat atgcagatcc tgcactgcag gcggccgcca ctctgagctg 60
aatgcagaag c 71
<210> 704
<211> 71
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 704
cttgacctcg gagaaagtta ccctcctttt gtcagacacg atcgatttcc aacagatgga 60
aagaagaaag g 71
<210> 705
<211> 61
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 705
aataccgccg cactggccgt agtgagactg ggtaggtcga ctaattccag ctgggatcat 60
a 61
<210> 706
<211> 62
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 706
cacactctca gaataaattc atcgccaatt tgacaggccg gccaattctc acggtgaatg 60
ta 62
<210> 707
<211> 58
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 707
ttagtagata gcgcacatag cgcgcatttg ttgacgccat gcggttgact attgggtt 58
<210> 708
<211> 24
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 708
ttcgagggcc gggagcgatg ccga 24
<210> 709
<211> 59
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 709
ggcttcaacg acctcgtcgg catcgctccc ggccctcgaa acgcgcccag cacctggca 59
<210> 710
<211> 60
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 710
ataccgccgc actggccgta gtgagactgg gtaggtcgac taattccagc taggatcata 60
<210> 711
<211> 69
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 711
agaataccgg attactgatc cggagaatac tttgtaaata gcggccgcgg ctgatgaggc 60
tgagagagg 69
<210> 712
<211> 58
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 712
caacgacctc gtcggcatcg ctcccggccc tcgaatgaac ggggatgccc tgtcgagc 58
<210> 713
<211> 69
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 713
gattaagttg ggtaacgcca gggttttccc agtcacgacg gtttaaactc caaagttcct 60
gtcttctcc 69
<210> 714
<211> 23
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 714
tgtcaaattg gcgatgaatt tat 23
<210> 715
<211> 69
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 715
ttattcacac tctcagaata aattcatcgc caatttgaca ggccggccgg gtaggaattg 60
tcactcaag 69
<210> 716
<211> 69
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 716
gcaacgagag cagagcagca gtagtcgatg ctaggcggcc gcggccgctc atcgtcttga 60
cagcaatgc 69
<210> 717
<211> 68
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 717
accagccgca gcctcagcct ctctcagcct catcagccgc gcggccgcca ctctgagctg 60
aatgcaga 68
<210> 718
<211> 66
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 718
cctatgttgt gtggaattgt gagcggataa caatttcaca gtttaaacgc gctgcctcca 60
cgatgt 66
<210> 719
<211> 78
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 719
gaccaacttg tccgttgcga ggccaacttg cattgctgtc aagacgatga gcggccgcat 60
aacggtgaga ctagcggc 78
<210> 720
<211> 72
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 720
atacaaacgt tggcgaggct tctgcattca gctcagagtg gcggccgcgt gctgcggaat 60
cattatcatc tg 72
<210> 721
<211> 70
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 721
gcagcttgac taacagctac cccgcttgag cagacatcat cttaattaat cagtcagcgc 60
tctcgatggc 70
<210> 722
<211> 70
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 722
ccaataccgc cgcactggcc gtagtgagac tgggtaggtc atttaaatct aattccagct 60
gggatcatag 70
<210> 723
<211> 62
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 723
gcagcttgac taacagctac cccgcttgag cagacatcat catgcgcttc cgaatctaca 60
ag 62
<210> 724
<211> 59
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 724
gggtgaggct ggcggggtcg ccatcgagag cgctgactga ggggtgatcc cctcccctg 59
<210> 725
<211> 99
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 725
gcagcttgac taacagctac cccgcttgag cagacatcat catggcgtca acaaatgcgc 60
gctatgtgcg ctatctacta atcgccttct tcacaatcc 99
<210> 726
<211> 85
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 726
tactaatcgc cttcttcaca atcctcgtct tctactttgt ctccaattca aagtatgagg 60
gcgtcgatct caacaagggc acctt 85
<210> 727
<211> 99
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 727
gggtgaggct ggcggggtcg ccatcgagag cgctgactga ctttggtgtc gtcttggtcg 60
aatccggagc tgtgaaggtg cccttgttga gatcgacgc 99
<210> 728
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 728
gcagcttgac taacagctac 20
<210> 729
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 729
gggtgaggct ggcggggtc 19
<210> 730
<211> 61
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 730
gcagcttgac taacagctac cccgcttgag cagacatcat catggccatt gcccggccgg 60
t 61
<210> 731
<211> 64
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 731
gggtgaggct ggcggggtcg ccatcgagag cgctgactga gcccgtggct atcaagaaga 60
agac 64
<210> 732
<211> 60
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 732
gcagcttgac taacagctac cccgcttgag cagacatcat catgttgaat ccacgccgcg 60
<210> 733
<211> 59
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 733
gggtgaggct ggcggggtcg ccatcgagag cgctgactga ggacgtcgag gccgattcg 59
<210> 734
<211> 61
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 734
gcagcttgac taacagctac cccgcttgag cagacatcat catgatgcca cggcatcact 60
c 61
<210> 735
<211> 60
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 735
gggtgaggct ggcggggtcg ccatcgagag cgctgactga ttcgagcttc aggtcatcgt 60
<210> 736
<211> 60
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 736
gcagcttgac taacagctac cccgcttgag cagacatcat catgctgctc cccaagggcg 60
<210> 737
<211> 61
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 737
gggtgaggct ggcggggtcg ccatcgagag cgctgactga ctcagatgcc gaagtggaca 60
c 61
<210> 738
<211> 116
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 738
cttgagcagt taattaattt gaatggccgg ccgcacactt tcaagattgg cttcaagaga 60
gccaatcttg aaagtgtgct tgcgatcgcg gatccactta acgttactga aatcat 116
<210> 739
<211> 116
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 739
atgatttcag taacgttaag tggatccgcg atcgcaagca cactttcaag attggctctc 60
ttgaagccaa tcttgaaagt gtgcggccgg ccattcaaat taattaactg ctcaag 116
<210> 740
<211> 116
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 740
cttgagcagt taattaattt gaatggccgg ccgtacggtg ttgccaagaa gttcaagaga 60
cttcttggca acaccgtact tgcgatcgcg gatccactta acgttactga aatcat 116
<210> 741
<211> 116
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 741
atgatttcag taacgttaag tggatccgcg atcgcaagta cggtgttgcc aagaagtctc 60
ttgaacttct tggcaacacc gtacggccgg ccattcaaat taattaactg ctcaag 116
<210> 742
<211> 67
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 742
ccgcttgagc agttaattaa tttaaatggc cggcctatat ggcgcgccgt aagttttgca 60
cagccgc 67
<210> 743
<211> 67
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 743
gtcaagctgt ttgatgattt cagtaacgtt aagtggatcc gcgatcgccg tactcgacgc 60
ccttgac 67
<210> 744
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 744
ccttattcct ttgaaccttt 20
<210> 745
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 745
gcaacgtgct caggagttgc 20
<210> 746
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 746
gcacactttc aagattggc 19
<210> 747
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 747
gtacggtgtt gccaagaag 19
<210> 748
<211> 448
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 748
gttgagtaca tcgagcgcga cagcattgtg cacaccatgc ttcccctcga gtccaaggac 60
agcatcatcg ttgaggactc gtgcaacggc gagacggaga agcaggctcc ctggggtctt 120
gcccgtatct ctcaccgaga gacgctcaac tttggctcct tcaacaagta cctctacacc 180
gctgatggtg gtgagggtgt tgatgcctat gtcattgaca ccggcaccaa catcgagcac 240
gtcgactttg agggtcgtgc caagtggggc aagaccatcc ctgccggcga tgaggacgag 300
gacggcaacg gccacggcac tcactgctct ggtaccgttg ctggtaagaa gtacggtgtt 360
gccaagaagg cccacgtcta cgccgtcaag gtgctccgat ccaacggatc cggcaccatg 420
tctgacgtcg tcaagggcgt cgagtacg 448
<210> 749
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 749
tcttgagcac gacaatcgac 20

Claims (12)

1.一种木霉属细胞，包含至少三种具有降低的或消除的活性的内源蛋白酶和编码异源多肽的重组多核苷酸，其中所述木霉属细胞至少在以下蛋白酶中具有降低或消除的活性：SEQ ID NO:118的gap1蛋白酶和至少另外两个选自SEQ ID NO:182的pep2蛋白酶和SEQ IDNO:166的slp3蛋白酶的蛋白酶，且其中所述异源多肽以比其中所述蛋白酶不具有降低的或消除的活性的对应亲本木霉属细胞中该多肽的产生水平高至少两倍的水平产生。
2.权利要求1所述的木霉属细胞，其中至少三个编码内源蛋白酶的基因各自包含消除相应蛋白酶活性的突变。
3.权利要求1或2所述的木霉属细胞，其中所述异源多肽是哺乳动物多肽。
4.权利要求3所述的木霉属细胞，其中所述哺乳动物多肽是糖基化的。
5.权利要求3所述的木霉属细胞，其中所述哺乳动物多肽选自抗体及其抗原结合片段、生长因子、干扰素、细胞因子和白介素。
6.权利要求3所述的木霉属细胞，其中所述哺乳动物多肽选自胰岛素样生长因子1(IGF1)、人生长激素(hGH)和干扰素α2b(IFNα2b)。
7.权利要求1所述的木霉属细胞，其中所述木霉属细胞进一步包含具有降低或消除的活性的ALG3。
8.权利要求1所述的木霉属细胞，其中所述木霉属细胞进一步包含编码α-1,2-甘露糖苷酶的多核苷酸。
9.权利要求1所述的木霉属细胞，进一步包含
N-乙酰葡糖胺基转移酶I催化结构域，和任选地
N-乙酰葡糖胺基转移酶II催化结构域。
10.权利要求1所述的木霉属细胞，进一步包含编码甘露糖苷酶II和/或半乳糖基转移酶的多核苷酸。
11.一种提高多肽的稳定性的方法，包括
a)提供权利要求1-10任一项的木霉属细胞，和
b)培养所述细胞以使得表达所述异源多肽，
其中所述异源多肽与其中所述蛋白酶不具有降低的或消除的活性的对应亲本木霉属细胞中产生的所述异源多肽相比表现出提高的稳定性。
12.一种制备异源多肽的方法，包括
提供权利要求1-10任一项的木霉属细胞，
培养所述细胞以使得表达所述异源多肽，和
纯化所述异源多肽。
CN201710503139.1A 2012-01-05 2013-01-04 蛋白酶缺陷丝状真菌细胞及其使用方法 Pending CN107868758A (zh)

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US61/583,559 2012-01-05
CN201380012940.6A CN104245919B (zh) 2012-01-05 2013-01-04 蛋白酶缺陷丝状真菌细胞及其使用方法

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