SG11201804966VA - Gene cassette for homologous recombination knock-out in yeast cells - Google Patents

Gene cassette for homologous recombination knock-out in yeast cells

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Publication number
SG11201804966VA
SG11201804966VA SG11201804966VA SG11201804966VA SG11201804966VA SG 11201804966V A SG11201804966V A SG 11201804966VA SG 11201804966V A SG11201804966V A SG 11201804966VA SG 11201804966V A SG11201804966V A SG 11201804966VA SG 11201804966V A SG11201804966V A SG 11201804966VA
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SG
Singapore
Prior art keywords
road
international
gda
gene
yeast cells
Prior art date
Application number
SG11201804966VA
Inventor
Xianzhong Chen
Lihua Zhang
Zheng Xiang
Wei Shen
Li Li
Markus Poetter
Original Assignee
Evonik Degussa China Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Evonik Degussa China Co Ltd filed Critical Evonik Degussa China Co Ltd
Publication of SG11201804966VA publication Critical patent/SG11201804966VA/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/165—Yeast isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/72—Candida
    • C12R2001/74—Candida tropicalis

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

b) .6 kb-URA3 PCR product Tramsformation 'MCA 0_3 kb-LA Oa 0.3 kb-RA... Integration 1.6 kb - URA3 03 kb -LA Oa .CAT2n(7,1 0.3 kb-RA chromosome- 'CAT2udR C ATR 0:3 kb-LA gda 0.3 kb-RA :'• ' ' 4 , Excision (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2017/101060 Al 22 June 2017 (22.06.2017) WIPO I PCT (51) International Patent Classification: C12N 15/81 (2006.01) CJ2R 1/74 (2006.01) C12N 15/60 (2006.01) (21) International Application Number: PCT/CN2015/097675 (22) International Filing Date: 17 December 2015 (17.12.2015) (25) Filing Language: English (26) Publication Language: English (71) Applicant: EVONIK DEGUSSA (CHINA) CO., LTD. [CN/CN]; 55 Chundong Road, Xinzhuang Industry Park, Shanghai 201108 (CN). (72) Inventors: CHEN, Xianzhong; Lihu Jiayuan 46-601, Lihu Road 1800, Wuxi, Jiangsu 214122 (CN). ZHANG, Lihua; Zhangdazhuang, Ganglidian Township, Xixian County, Xinyang, Henan 464314 (CN). XIANG, Zheng; Yiquany- uan 25-501, Rongxiang Street, Binhu District, Wuxi, Ji- angsu 214000 (CN). SHEN, Wei; Wankemeili City 71- 403, Zhouxin Road, Wuxi, Jiangsu 214122 (CN). LI, Li; 1517 Huqingping Road, House 129, Shanghai 201702 (CN). POETTER, Markus; 658 Gaojing Road, House #85, Shanghai 201702 (CN). (74) Agent: CHINA PATENT AGENT (H.K.) LTD.; 22/F., Great Eagle Center, 23 Harbour Road, Wanchai, Hong Kong (CN). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) [Continued on next page] (54) Title: GENE CASSETTE FOR HOMOLOGOUS RECOMBINATION KNOCK-OUT IN YEAST CELLS 1111111111111101110101011111010111110 III 0101111101011011001111111111111110111111 (57) : A gene cassette for the knock-out of at least on target gene in yeast cells, the cassette comprises: (a) a URA3 gene as a marker gene; (b) at least one gene disruption auxiliary (gda) se- quence; and (c) the upstream and down- stream sequences of the target gene, wherein the gda sequence is a sequence having at least 300 to 600 consective residues of the nucleotide sequence of SEQ ID NO: 39. 0.3 kb-LA gda 0,3 kb - RA giSiV i • \ \ \ cAmdu ?ndk 0.3 kb-LA gda 0.3 kb- W O 20 17 / 10 1060 Al FIGURE 2 WO 2017/101060 Al 11111111111111011101111111111111111101111111111111111111111111111110N1011110111111 — with sequence listing part of description (Rule 5.2(a))
SG11201804966VA 2015-12-17 2015-12-17 Gene cassette for homologous recombination knock-out in yeast cells SG11201804966VA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2015/097675 WO2017101060A1 (en) 2015-12-17 2015-12-17 Gene cassette for homologous recombination knock-out in yeast cells

Publications (1)

Publication Number Publication Date
SG11201804966VA true SG11201804966VA (en) 2018-07-30

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Country Status (6)

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US (1) US20190153475A1 (en)
EP (1) EP3390640A4 (en)
JP (1) JP6858191B2 (en)
CN (1) CN108779470A (en)
SG (1) SG11201804966VA (en)
WO (1) WO2017101060A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11201804966VA (en) * 2015-12-17 2018-07-30 Evonik Degussa China Co Ltd Gene cassette for homologous recombination knock-out in yeast cells
CN112175984A (en) * 2020-09-18 2021-01-05 中国科学院深圳先进技术研究院 Molecular cloning method based on synthetic gene and saccharomyces cerevisiae homologous recombination mechanism
CN114438163B (en) * 2022-01-27 2024-05-14 深圳瑞德林生物技术有限公司 Fungus screening reagent, screening method, kit and application

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EP1352968A1 (en) * 1997-09-09 2003-10-15 BIOCHEMIE Gesellschaft m.b.H. Esterase free enzymes
DE69835104D1 (en) * 1997-12-08 2006-08-10 Seminis Vegetables Seeds Inc A starch-free pisum sativum plant with increased sucrose content
AU2003247411A1 (en) * 2002-05-23 2003-12-12 Cognis Corporation NON-REVERTIBLE Beta-OXIDATION BLOCKED CANDIDA TROPICALIS
DK2338895T3 (en) * 2003-05-02 2013-09-08 Cargill Inc Genetically modified yeast species and fermentation process using genetically modified yeast
JP4874124B2 (en) * 2004-01-30 2012-02-15 ミクシス フランス ソシエテ アノニム Production of recombinant genes in Saccharomyces cerevisiae
DE102005048818A1 (en) * 2005-10-10 2007-04-12 Degussa Ag Microbiological production of 3-hydroxypropionic acid
DE102009046626A1 (en) * 2009-11-11 2011-05-12 Evonik Degussa Gmbh Candida tropicalis cells and their use
CN102061296A (en) * 2010-08-20 2011-05-18 吉林农业大学 Preparation method of water-soluble human collagen VI polypeptide
WO2012176016A1 (en) * 2011-06-20 2012-12-27 Amikana Biologics Cassettes and methods for transforming and selecting yeast transformants by homologous recombination
EP3358000A3 (en) * 2012-01-05 2018-12-19 Glykos Finland Oy Protease deficient filamentous fungal cells and methods of use thereof
SG11201504838YA (en) * 2012-12-19 2015-07-30 Verdezyne Inc Biological methods for preparing a fatty dicarboxylic acid
CN103232947B (en) * 2013-04-12 2015-06-24 天津科技大学 Freezing resistant Saccharomyces cerevisiae bacterial strain and construction method thereof
KR20160035587A (en) * 2013-07-10 2016-03-31 노파르티스 아게 Multiple proteases deficient filamentous fungal cells and methods of use thereof
CN105779490A (en) * 2014-12-16 2016-07-20 北京集智新创科技有限公司 Construction method of Pichia pastoris expressed by OCH1 defect anti-CD20 tetravalent antibody
CN104962488B (en) * 2015-07-22 2019-03-05 天津大学 A kind of restructuring yeast strains and its construction method and application
CN105062908A (en) * 2015-08-13 2015-11-18 江南大学 Candida tropicalis gene engineering bacteria for high yield of xylitol and application of xylitol
CN105112313B (en) * 2015-08-28 2019-04-05 复旦大学 Kluyveromyces marxianus auxotrophic strain and seamless genome manipulation method
SG11201804966VA (en) * 2015-12-17 2018-07-30 Evonik Degussa China Co Ltd Gene cassette for homologous recombination knock-out in yeast cells
CN108410902B (en) * 2018-01-24 2021-05-18 齐鲁工业大学 Novel saccharomyces cerevisiae expression system and construction method thereof
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CN110452865B (en) * 2019-08-15 2021-05-28 江南大学 Recombinant escherichia coli for producing tyrosol as well as construction method and application thereof
CN112941119B (en) * 2021-01-22 2022-08-30 江南大学 Method for increasing yield of fatty acid ethyl ester of saccharomyces cerevisiae engineering bacteria

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WO2017101060A1 (en) 2017-06-22
JP6858191B2 (en) 2021-04-14
US20190153475A1 (en) 2019-05-23
CN108779470A (en) 2018-11-09
EP3390640A4 (en) 2019-05-22
EP3390640A1 (en) 2018-10-24
JP2018537109A (en) 2018-12-20

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