JP2016512848A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2016512848A5 JP2016512848A5 JP2016503420A JP2016503420A JP2016512848A5 JP 2016512848 A5 JP2016512848 A5 JP 2016512848A5 JP 2016503420 A JP2016503420 A JP 2016503420A JP 2016503420 A JP2016503420 A JP 2016503420A JP 2016512848 A5 JP2016512848 A5 JP 2016512848A5
- Authority
- JP
- Japan
- Prior art keywords
- recombinant polypeptide
- antibody
- eluate
- impurities
- support
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229920001184 polypeptide Polymers 0.000 claims 44
- 102000004196 processed proteins & peptides Human genes 0.000 claims 44
- 108090000765 processed proteins & peptides Proteins 0.000 claims 44
- 239000012535 impurity Substances 0.000 claims 31
- 238000000034 method Methods 0.000 claims 27
- 108090001090 Lectins Proteins 0.000 claims 17
- 102000004856 Lectins Human genes 0.000 claims 17
- 239000002523 lectin Substances 0.000 claims 17
- 210000004027 cell Anatomy 0.000 claims 15
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims 11
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims 11
- 238000000855 fermentation Methods 0.000 claims 11
- 230000004151 fermentation Effects 0.000 claims 11
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims 11
- 241000235648 Pichia Species 0.000 claims 10
- -1 ML-I-III Proteins 0.000 claims 9
- 102000004169 proteins and genes Human genes 0.000 claims 8
- 108090000623 proteins and genes Proteins 0.000 claims 8
- 229920002684 Sepharose Polymers 0.000 claims 7
- 238000001514 detection method Methods 0.000 claims 7
- 238000012544 monitoring process Methods 0.000 claims 7
- 101001007348 Arachis hypogaea Galactose-binding lectin Proteins 0.000 claims 6
- 238000002965 ELISA Methods 0.000 claims 6
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical group CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims 6
- 241000235058 Komagataella pastoris Species 0.000 claims 5
- 230000002538 fungal effect Effects 0.000 claims 5
- 238000004191 hydrophobic interaction chromatography Methods 0.000 claims 5
- 239000003446 ligand Substances 0.000 claims 5
- 239000002609 medium Substances 0.000 claims 5
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 claims 4
- 108010037897 DC-specific ICAM-3 grabbing nonintegrin Proteins 0.000 claims 4
- 241000233866 Fungi Species 0.000 claims 4
- 241000223218 Fusarium Species 0.000 claims 4
- 241000283973 Oryctolagus cuniculus Species 0.000 claims 4
- 241000228143 Penicillium Species 0.000 claims 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 4
- 238000001042 affinity chromatography Methods 0.000 claims 4
- 230000036252 glycation Effects 0.000 claims 4
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims 4
- 238000012434 mixed-mode chromatography Methods 0.000 claims 4
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims 4
- 230000004850 protein–protein interaction Effects 0.000 claims 4
- 239000001488 sodium phosphate Substances 0.000 claims 4
- 229910000162 sodium phosphate Inorganic materials 0.000 claims 4
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 claims 4
- 101100022253 Aedes aegypti MAL1 gene Proteins 0.000 claims 3
- 241000228212 Aspergillus Species 0.000 claims 3
- 102100028668 C-type lectin domain family 4 member C Human genes 0.000 claims 3
- 102100028681 C-type lectin domain family 4 member K Human genes 0.000 claims 3
- 101710183165 C-type lectin domain family 4 member K Proteins 0.000 claims 3
- 102100040843 C-type lectin domain family 4 member M Human genes 0.000 claims 3
- 102100040839 C-type lectin domain family 6 member A Human genes 0.000 claims 3
- 101710125370 C-type lectin domain family 6 member A Proteins 0.000 claims 3
- 102100040840 C-type lectin domain family 7 member A Human genes 0.000 claims 3
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims 3
- 101100005933 Drosophila melanogaster vvl gene Proteins 0.000 claims 3
- 101000766907 Homo sapiens C-type lectin domain family 4 member C Proteins 0.000 claims 3
- 101000749311 Homo sapiens C-type lectin domain family 4 member M Proteins 0.000 claims 3
- 102100033486 Lymphocyte antigen 75 Human genes 0.000 claims 3
- 101710157884 Lymphocyte antigen 75 Proteins 0.000 claims 3
- 102100025354 Macrophage mannose receptor 1 Human genes 0.000 claims 3
- 101710124692 Macrophage mannose receptor 1 Proteins 0.000 claims 3
- 241000235527 Rhizopus Species 0.000 claims 3
- 241000223259 Trichoderma Species 0.000 claims 3
- 101150099477 ail gene Proteins 0.000 claims 3
- 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 claims 3
- 239000000919 ceramic Substances 0.000 claims 3
- 238000004587 chromatography analysis Methods 0.000 claims 3
- 108010025838 dectin 1 Proteins 0.000 claims 3
- 238000002296 dynamic light scattering Methods 0.000 claims 3
- 239000012634 fragment Substances 0.000 claims 3
- 230000013595 glycosylation Effects 0.000 claims 3
- 238000006206 glycosylation reaction Methods 0.000 claims 3
- 108010084553 jacalin Proteins 0.000 claims 3
- 230000010287 polarization Effects 0.000 claims 3
- 238000000746 purification Methods 0.000 claims 3
- 238000001542 size-exclusion chromatography Methods 0.000 claims 3
- 238000001370 static light scattering Methods 0.000 claims 3
- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 claims 3
- 102400000344 Angiotensin-1 Human genes 0.000 claims 2
- 101800000734 Angiotensin-1 Proteins 0.000 claims 2
- 102400000345 Angiotensin-2 Human genes 0.000 claims 2
- 101800000733 Angiotensin-2 Proteins 0.000 claims 2
- 108010064733 Angiotensins Proteins 0.000 claims 2
- 102000015427 Angiotensins Human genes 0.000 claims 2
- 241001508785 Arxiozyma Species 0.000 claims 2
- 102100024506 Bone morphogenetic protein 2 Human genes 0.000 claims 2
- 102100022544 Bone morphogenetic protein 7 Human genes 0.000 claims 2
- 241000283690 Bos taurus Species 0.000 claims 2
- 241000178289 Botryozyma Species 0.000 claims 2
- 102100025248 C-X-C motif chemokine 10 Human genes 0.000 claims 2
- 101710098275 C-X-C motif chemokine 10 Proteins 0.000 claims 2
- 102100025277 C-X-C motif chemokine 13 Human genes 0.000 claims 2
- 108090000932 Calcitonin Gene-Related Peptide Proteins 0.000 claims 2
- 102100025588 Calcitonin gene-related peptide 1 Human genes 0.000 claims 2
- 241000283707 Capra Species 0.000 claims 2
- 241001508787 Citeromyces Species 0.000 claims 2
- 241000221760 Claviceps Species 0.000 claims 2
- 102000004127 Cytokines Human genes 0.000 claims 2
- 108090000695 Cytokines Proteins 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 2
- 102100021866 Hepatocyte growth factor Human genes 0.000 claims 2
- 101000762366 Homo sapiens Bone morphogenetic protein 2 Proteins 0.000 claims 2
- 101000899361 Homo sapiens Bone morphogenetic protein 7 Proteins 0.000 claims 2
- 101000858064 Homo sapiens C-X-C motif chemokine 13 Proteins 0.000 claims 2
- 101000898034 Homo sapiens Hepatocyte growth factor Proteins 0.000 claims 2
- 101001076408 Homo sapiens Interleukin-6 Proteins 0.000 claims 2
- 101001098868 Homo sapiens Proprotein convertase subtilisin/kexin type 9 Proteins 0.000 claims 2
- 101000654356 Homo sapiens Sodium channel protein type 10 subunit alpha Proteins 0.000 claims 2
- 101000654386 Homo sapiens Sodium channel protein type 9 subunit alpha Proteins 0.000 claims 2
- 101000868152 Homo sapiens Son of sevenless homolog 1 Proteins 0.000 claims 2
- 101000611183 Homo sapiens Tumor necrosis factor Proteins 0.000 claims 2
- CZGUSIXMZVURDU-JZXHSEFVSA-N Ile(5)-angiotensin II Chemical compound C([C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC=1C=CC=CC=1)C([O-])=O)NC(=O)[C@@H](NC(=O)[C@H](CCCNC(N)=[NH2+])NC(=O)[C@@H]([NH3+])CC([O-])=O)C(C)C)C1=CC=C(O)C=C1 CZGUSIXMZVURDU-JZXHSEFVSA-N 0.000 claims 2
- 102100037850 Interferon gamma Human genes 0.000 claims 2
- 102000006992 Interferon-alpha Human genes 0.000 claims 2
- 108010047761 Interferon-alpha Proteins 0.000 claims 2
- 108010074328 Interferon-gamma Proteins 0.000 claims 2
- 102000003814 Interleukin-10 Human genes 0.000 claims 2
- 108090000174 Interleukin-10 Proteins 0.000 claims 2
- 102000013462 Interleukin-12 Human genes 0.000 claims 2
- 108010065805 Interleukin-12 Proteins 0.000 claims 2
- 102000013691 Interleukin-17 Human genes 0.000 claims 2
- 108050003558 Interleukin-17 Proteins 0.000 claims 2
- 102000003810 Interleukin-18 Human genes 0.000 claims 2
- 108090000171 Interleukin-18 Proteins 0.000 claims 2
- 108010002350 Interleukin-2 Proteins 0.000 claims 2
- 102000000588 Interleukin-2 Human genes 0.000 claims 2
- 102000004388 Interleukin-4 Human genes 0.000 claims 2
- 108090000978 Interleukin-4 Proteins 0.000 claims 2
- 102000004889 Interleukin-6 Human genes 0.000 claims 2
- 108090001005 Interleukin-6 Proteins 0.000 claims 2
- 241000235649 Kluyveromyces Species 0.000 claims 2
- 241001302784 Kodamaea Species 0.000 claims 2
- 241000221479 Leucosporidium Species 0.000 claims 2
- 241000699666 Mus <mouse, genus> Species 0.000 claims 2
- 102000015336 Nerve Growth Factor Human genes 0.000 claims 2
- 108010025020 Nerve Growth Factor Proteins 0.000 claims 2
- 241000221960 Neurospora Species 0.000 claims 2
- 230000004989 O-glycosylation Effects 0.000 claims 2
- 241001236817 Paecilomyces <Clavicipitaceae> Species 0.000 claims 2
- 241001494479 Pecora Species 0.000 claims 2
- 102100033237 Pro-epidermal growth factor Human genes 0.000 claims 2
- 102100022661 Pro-neuregulin-1, membrane-bound isoform Human genes 0.000 claims 2
- 102100038955 Proprotein convertase subtilisin/kexin type 9 Human genes 0.000 claims 2
- 241000700159 Rattus Species 0.000 claims 2
- 102100031374 Sodium channel protein type 10 subunit alpha Human genes 0.000 claims 2
- 102100031367 Sodium channel protein type 9 subunit alpha Human genes 0.000 claims 2
- 241000235006 Torulaspora Species 0.000 claims 2
- 102000040945 Transcription factor Human genes 0.000 claims 2
- 108091023040 Transcription factor Proteins 0.000 claims 2
- 102100040247 Tumor necrosis factor Human genes 0.000 claims 2
- 102100036922 Tumor necrosis factor ligand superfamily member 13B Human genes 0.000 claims 2
- 101710181056 Tumor necrosis factor ligand superfamily member 13B Proteins 0.000 claims 2
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 claims 2
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 claims 2
- 241000235152 Williopsis Species 0.000 claims 2
- 241000235013 Yarrowia Species 0.000 claims 2
- 241000235017 Zygosaccharomyces Species 0.000 claims 2
- 238000005273 aeration Methods 0.000 claims 2
- 238000013019 agitation Methods 0.000 claims 2
- ORWYRWWVDCYOMK-HBZPZAIKSA-N angiotensin I Chemical compound C([C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CC(C)C)C(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@@H](N)CC(O)=O)C(C)C)C1=CC=C(O)C=C1 ORWYRWWVDCYOMK-HBZPZAIKSA-N 0.000 claims 2
- 229950006323 angiotensin ii Drugs 0.000 claims 2
- 238000012258 culturing Methods 0.000 claims 2
- 230000000694 effects Effects 0.000 claims 2
- 238000010828 elution Methods 0.000 claims 2
- 229910052587 fluorapatite Inorganic materials 0.000 claims 2
- 239000003102 growth factor Substances 0.000 claims 2
- 229920000140 heteropolymer Polymers 0.000 claims 2
- 108010044853 histidine-rich proteins Proteins 0.000 claims 2
- 229920001519 homopolymer Polymers 0.000 claims 2
- 239000005556 hormone Substances 0.000 claims 2
- 229940088597 hormone Drugs 0.000 claims 2
- 244000005700 microbiome Species 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 238000000569 multi-angle light scattering Methods 0.000 claims 2
- OHDXDNUPVVYWOV-UHFFFAOYSA-N n-methyl-1-(2-naphthalen-1-ylsulfanylphenyl)methanamine Chemical compound CNCC1=CC=CC=C1SC1=CC=CC2=CC=CC=C12 OHDXDNUPVVYWOV-UHFFFAOYSA-N 0.000 claims 2
- 102000039446 nucleic acids Human genes 0.000 claims 2
- 108020004707 nucleic acids Proteins 0.000 claims 2
- 150000007523 nucleic acids Chemical class 0.000 claims 2
- 125000002347 octyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 claims 2
- 238000011176 pooling Methods 0.000 claims 2
- 230000028327 secretion Effects 0.000 claims 2
- 239000011780 sodium chloride Substances 0.000 claims 2
- 210000005253 yeast cell Anatomy 0.000 claims 2
- GUPXYSSGJWIURR-UHFFFAOYSA-N 3-octoxypropane-1,2-diol Chemical compound CCCCCCCCOCC(O)CO GUPXYSSGJWIURR-UHFFFAOYSA-N 0.000 claims 1
- 239000004475 Arginine Substances 0.000 claims 1
- 241000235172 Bullera Species 0.000 claims 1
- 239000012619 Butyl Sepharose® Substances 0.000 claims 1
- 241000235035 Debaryomyces Species 0.000 claims 1
- 102000003816 Interleukin-13 Human genes 0.000 claims 1
- 108090000176 Interleukin-13 Proteins 0.000 claims 1
- 241000235644 Issatchenkia Species 0.000 claims 1
- 241001233945 Kazachstania Species 0.000 claims 1
- 241001452677 Ogataea methanolica Species 0.000 claims 1
- 241000235652 Pachysolen Species 0.000 claims 1
- 241000223252 Rhodotorula Species 0.000 claims 1
- 241000235070 Saccharomyces Species 0.000 claims 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims 1
- 241000228389 Sporidiobolus Species 0.000 claims 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims 1
- 238000000149 argon plasma sintering Methods 0.000 claims 1
- 238000003556 assay Methods 0.000 claims 1
- 238000011097 chromatography purification Methods 0.000 claims 1
- 230000008878 coupling Effects 0.000 claims 1
- 238000010168 coupling process Methods 0.000 claims 1
- 238000005859 coupling reaction Methods 0.000 claims 1
- 150000004676 glycans Chemical class 0.000 claims 1
- 238000003367 kinetic assay Methods 0.000 claims 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims 1
- 239000011148 porous material Substances 0.000 claims 1
- 230000006916 protein interaction Effects 0.000 claims 1
- 229910052938 sodium sulfate Inorganic materials 0.000 claims 1
- 235000011152 sodium sulphate Nutrition 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361792935P | 2013-03-15 | 2013-03-15 | |
| US61/792,935 | 2013-03-15 | ||
| PCT/US2014/030558 WO2014145744A1 (en) | 2013-03-15 | 2014-03-17 | Antibody purification and purity monitoring |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016512848A JP2016512848A (ja) | 2016-05-09 |
| JP2016512848A5 true JP2016512848A5 (enExample) | 2017-04-20 |
| JP6472786B2 JP6472786B2 (ja) | 2019-02-20 |
Family
ID=51538079
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016503420A Active JP6472786B2 (ja) | 2013-03-15 | 2014-03-17 | 抗体の精製及び純度のモニタリング |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US9518082B2 (enExample) |
| EP (2) | EP3754012A1 (enExample) |
| JP (1) | JP6472786B2 (enExample) |
| AR (1) | AR095615A1 (enExample) |
| CA (1) | CA2905531C (enExample) |
| ES (1) | ES2834113T3 (enExample) |
| TW (2) | TWI670279B (enExample) |
| WO (1) | WO2014145744A1 (enExample) |
Families Citing this family (53)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2702077A2 (en) | 2011-04-27 | 2014-03-05 | AbbVie Inc. | Methods for controlling the galactosylation profile of recombinantly-expressed proteins |
| WO2013158273A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Methods to modulate c-terminal lysine variant distribution |
| US9067990B2 (en) | 2013-03-14 | 2015-06-30 | Abbvie, Inc. | Protein purification using displacement chromatography |
| WO2013158279A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Protein purification methods to reduce acidic species |
| US9255154B2 (en) | 2012-05-08 | 2016-02-09 | Alderbio Holdings, Llc | Anti-PCSK9 antibodies and use thereof |
| US9512214B2 (en) | 2012-09-02 | 2016-12-06 | Abbvie, Inc. | Methods to control protein heterogeneity |
| CA2905010A1 (en) | 2013-03-12 | 2014-09-18 | Abbvie Inc. | Human antibodies that bind human tnf-alpha and methods of preparing the same |
| US10023608B1 (en) | 2013-03-13 | 2018-07-17 | Amgen Inc. | Protein purification methods to remove impurities |
| US9017687B1 (en) | 2013-10-18 | 2015-04-28 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same using displacement chromatography |
| WO2014151878A2 (en) | 2013-03-14 | 2014-09-25 | Abbvie Inc. | Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides |
| ES2834113T3 (es) * | 2013-03-15 | 2021-06-16 | H Lundbeck As | Purificación de anticuerpos y monitorización de la pureza |
| US9598667B2 (en) | 2013-10-04 | 2017-03-21 | Abbvie Inc. | Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins |
| US9181337B2 (en) | 2013-10-18 | 2015-11-10 | Abbvie, Inc. | Modulated lysine variant species compositions and methods for producing and using the same |
| US9085618B2 (en) | 2013-10-18 | 2015-07-21 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same |
| US20150139988A1 (en) | 2013-11-15 | 2015-05-21 | Abbvie, Inc. | Glycoengineered binding protein compositions |
| CA2954767A1 (en) * | 2014-07-14 | 2016-01-21 | Amgen Inc. | Crystalline antibody formulations |
| TW201628649A (zh) | 2014-10-09 | 2016-08-16 | 再生元醫藥公司 | 減少醫藥調配物中微可見顆粒之方法 |
| CN107148283A (zh) * | 2014-10-31 | 2017-09-08 | 豪夫迈·罗氏有限公司 | 抗il‑17a和il‑17f交叉反应性抗体变体、包含其的组合物及其制备和使用方法 |
| EP3334747B1 (en) | 2015-08-13 | 2023-09-27 | Amgen Inc. | Charged depth filtration of antigen-binding proteins |
| EP3344643B1 (en) | 2015-09-04 | 2025-07-02 | Qiagen Sciences LLC | Methods for co-isolation of nucleic acids and proteins |
| AU2017229635A1 (en) * | 2016-03-07 | 2018-09-20 | Hitachi Chemical Advanced Therapeutics Solutions, LLC | A closed system for labelling and selecting live cells |
| JP2019515671A (ja) | 2016-04-15 | 2019-06-13 | アルダー バイオファーマシューティカルズ、インコーポレイテッド | 抗ｐａｃａｐ抗体及びそれらの使用 |
| KR102369014B1 (ko) | 2016-08-16 | 2022-03-02 | 리제너론 파아마슈티컬스, 인크. | 혼합물로부터 개별 항체들을 정량하는 방법 |
| CN106371765B (zh) * | 2016-08-29 | 2020-09-18 | 成都科鸿达科技有限公司 | 一种高效大规模系统的ltl模型检测去内存抖动的方法 |
| CN106293544B (zh) * | 2016-08-29 | 2020-06-30 | 成都科鸿达科技有限公司 | 一种高效大规模系统的ltl模型检测方法 |
| ES3032778T3 (en) | 2016-10-25 | 2025-07-24 | Regeneron Pharma | Methods for chromatography data analysis |
| JP6829357B2 (ja) * | 2017-03-17 | 2021-02-10 | 住友ベークライト株式会社 | 抗体の糖鎖検出法 |
| CN107033236B (zh) * | 2017-05-05 | 2021-03-23 | 浙江大学 | 一种从酵母发酵液中分离人血白蛋白的混合模式层析方法 |
| CN107254502A (zh) * | 2017-07-26 | 2017-10-17 | 兰州名德农牧科技有限公司 | 一种动物蛋白酶制备羊胎盘肽的方法 |
| US11697670B2 (en) | 2017-08-22 | 2023-07-11 | Biogen Ma Inc. | Methods for purifying antibodies having reduced high molecular weight aggregates |
| MY203415A (en) | 2017-09-19 | 2024-06-27 | Regeneron Pharma | Methods of reducing particle formation and compositions formed thereby |
| US20210024873A1 (en) * | 2018-03-27 | 2021-01-28 | Bristol-Myers Squibb Company | Real-time monitoring of protein concentration using ultraviolet signal |
| US12018075B2 (en) | 2018-04-20 | 2024-06-25 | Janssen Biotech, Inc. | Chromatography column qualification in manufacturing methods for producing anti-TNF antibody compositions |
| AU2019260569B9 (en) | 2018-04-24 | 2025-12-11 | Qiagen Sciences Llc | Nucleic acid isolation and inhibitor removal from complex samples |
| GB201806752D0 (en) * | 2018-04-25 | 2018-06-06 | Ge Healthcare Bioprocess R&D Ab | Method in bioprocess system |
| TW202448568A (zh) | 2018-07-02 | 2024-12-16 | 美商里珍納龍藥品有限公司 | 自混合物製備多肽之系統及方法 |
| BR112021013734A2 (pt) | 2019-01-30 | 2021-09-21 | Regeneron Pharmaceuticals, Inc. | Método para caracterizar partículas visíveis e/ou subvisíveis em biofármacos |
| TW202532844A (zh) * | 2019-09-23 | 2025-08-16 | 美商健臻公司 | 用於測量生物樣品的分析物的多個產品品質屬性的方法 |
| CN111239408B (zh) * | 2020-01-19 | 2023-02-10 | 深圳格道糖生物技术有限公司 | 特定凝集素组合在构建基于唾液糖蛋白糖链鉴别肝纤维化的测试工具方面的应用 |
| US20230058276A1 (en) * | 2020-01-24 | 2023-02-23 | Amgen Inc. | Methods for harvesting biomolecules |
| JP2020099909A (ja) * | 2020-03-17 | 2020-07-02 | ＨＯＹＡ Ｔｅｃｈｎｏｓｕｒｇｉｃａｌ株式会社 | 処理方法、生産方法およびハイドロキシアパタイト充填剤 |
| US20230167153A1 (en) * | 2020-05-01 | 2023-06-01 | Kashiv Biosciences, Llc | An improved process of purification of protein |
| CN111579777B (zh) * | 2020-05-29 | 2024-02-02 | 江苏省苏微微生物研究有限公司 | 一种以大粒径凝胶为载体的亲和介质及其应用 |
| AU2021381371A1 (en) * | 2020-11-18 | 2023-06-22 | Modernatx, Inc. | Ribonucleic acid purification |
| GB202105559D0 (en) * | 2021-04-19 | 2021-06-02 | Cytiva Bioprocess R & D Ab | Method of clarifying a crude protein solution |
| US20240383945A1 (en) * | 2021-05-10 | 2024-11-21 | Chiome Bioscience Inc. | Purification method of antibody composition |
| CN113416243B (zh) * | 2021-08-23 | 2021-12-10 | 上海盛迪医药有限公司 | 一种纯化抗体的方法 |
| CN114279968B (zh) * | 2021-12-28 | 2024-09-20 | 中国科学院长春应用化学研究所 | 一种电化学-双偏振干涉光电检测池 |
| CN116159340A (zh) * | 2022-12-16 | 2023-05-26 | 浙江大学 | 一种用于体积排阻色谱法的缓冲液 |
| WO2025155751A1 (en) * | 2024-01-17 | 2025-07-24 | Nutcracker Therapeutics, Inc. | Improved method for isolating and characterizing proteins |
| CN118006716B (zh) * | 2024-03-15 | 2024-09-10 | 通化安睿特生物制药股份有限公司 | 制备高表达且低o-糖基化水平的重组人白蛋白的方法 |
| CN119000228B (zh) * | 2024-08-26 | 2025-02-28 | 西南大学 | 蛋白质组学样品前处理清洗装置 |
| CN119832975A (zh) * | 2024-12-24 | 2025-04-15 | 上海药明生物技术有限公司 | 一种蛋白质共处方制剂的稳定性预测方法 |
Family Cites Families (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4855231A (en) | 1984-10-30 | 1989-08-08 | Phillips Petroleum Company | Regulatory region for heterologous gene expression in yeast |
| US4818700A (en) | 1985-10-25 | 1989-04-04 | Phillips Petroleum Company | Pichia pastoris argininosuccinate lyase gene and uses thereof |
| US4812405A (en) | 1986-02-18 | 1989-03-14 | Phillips Petroleum Company | Double auxotrophic mutants of Pichia pastoris and methods for preparation |
| US4929555A (en) | 1987-10-19 | 1990-05-29 | Phillips Petroleum Company | Pichia transformation |
| WO1994021293A1 (en) | 1993-03-19 | 1994-09-29 | Duke University | Method of treatment of tumors with an antibody binding to tenascin |
| US5429746A (en) * | 1994-02-22 | 1995-07-04 | Smith Kline Beecham Corporation | Antibody purification |
| ATE306930T1 (de) | 1994-08-12 | 2005-11-15 | Immunomedics Inc | Für b-zell-lymphom und leukämiezellen spezifische immunkonjugate und humane antikörper |
| US5955349A (en) | 1996-08-26 | 1999-09-21 | Zymogenetics, Inc. | Compositions and methods for producing heterologous polypeptides in Pichia methanolica |
| US5736383A (en) | 1996-08-26 | 1998-04-07 | Zymogenetics, Inc. | Preparation of Pichia methanolica auxotrophic mutants |
| US6730499B1 (en) | 1998-07-03 | 2004-05-04 | Research Corporation Technologies, Inc. | Promoter for the Pichia pastoris formaldehyde dehydrogenase gene FLD1 |
| US6258559B1 (en) | 1999-03-22 | 2001-07-10 | Zymogenetics, Inc. | Method for producing proteins in transformed Pichia |
| US20030091976A1 (en) | 2001-11-14 | 2003-05-15 | Ciphergen Biosystems, Inc. | Methods for monitoring polypeptide production and purification using surface enhanced laser desorption/ionization mass spectrometry |
| JPWO2004087761A1 (ja) * | 2003-03-31 | 2006-07-27 | 麒麟麦酒株式会社 | ヒトモノクローナル抗体およびヒトポリクローナル抗体の精製 |
| WO2004090549A1 (en) * | 2003-04-11 | 2004-10-21 | Novozymes A/S | Method of screening for secreted glycosylated polypeptides |
| HUE033349T2 (en) | 2003-10-22 | 2017-11-28 | Keck Graduate Inst | Procedures for the Synthesis of Heteromultimer Polypeptides in Yeast Using an Hagloid Mating Strategy |
| KR101482791B1 (ko) * | 2005-03-11 | 2015-01-21 | 와이어쓰 엘엘씨 | 약한 분배성 크로마토그래피법 |
| MX2008014692A (es) | 2006-05-19 | 2009-08-18 | Alder Biopharmaceuticals Inc | Metodo de cultivo para obtener una poblacion clonal de celulas b especificas de antigeno. |
| HRP20150307T1 (hr) * | 2006-09-10 | 2015-04-24 | Glycotope Gmbh | Upotreba ljudskih stanica podrijetlom iz ljudske leukemije za eksprimiranje protutijela |
| CN101646775B (zh) * | 2006-12-28 | 2016-08-24 | 森托科尔奥索生物科技公司 | 用于产生脱唾液酸化免疫球蛋白的方法和载体 |
| EP2508613A3 (en) * | 2007-04-03 | 2012-11-28 | Oxyrane UK Limited | Glycosylation of molecules |
| MX343879B (es) | 2007-05-21 | 2016-11-25 | Alderbio Holdings Llc | Metodo de humanizacion de anticuerpo de conejo novedoso y anticuerpos de conejo humanizados. |
| ES2721753T3 (es) | 2007-05-21 | 2019-08-05 | Alderbio Holdings Llc | Anticuerpos contra IL-6 y usos de los mismos |
| RU2010112236A (ru) | 2007-08-31 | 2011-10-10 | Ф.Хоффманн-Ля Рош Аг (Ch) | Анализ профиля гликозилирования |
| EP2490780A4 (en) * | 2009-10-20 | 2014-04-09 | Merck Sharp & Dohme | USE OF A MIXED MODE CHROMATOGRAPHY FOR THE DETECTION AND PURIFICATION OF BASIC ANTIBODY PRODUCTS |
| SG185601A1 (en) * | 2010-05-18 | 2012-12-28 | Abbvie Inc | Apparatus and process of purification of proteins |
| US11214610B2 (en) | 2010-12-01 | 2022-01-04 | H. Lundbeck A/S | High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris |
| US9745373B2 (en) | 2011-05-20 | 2017-08-29 | Alderbio Holdings Llc | Anti-CGRP compositions and use thereof |
| WO2013013193A1 (en) * | 2011-07-20 | 2013-01-24 | Zepteon, Incorporated | Polypeptide separation methods |
| US10150968B2 (en) | 2011-08-19 | 2018-12-11 | Alderbio Holdings Llc | Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris |
| KR101978534B1 (ko) * | 2011-08-19 | 2019-08-28 | 앨더바이오 홀딩스 엘엘씨 | 변형된 미생물 이를 테면 피치아 파스토리스(pichia pastoris)에서 다수의 아단위 단백질들, 이를 테면 항체의 고역가 및 고순도 생산을 위한 다중-복사체 전략 |
| EP2773439A4 (en) * | 2011-10-31 | 2015-07-01 | Merck Sharp & Dohme | CHROMATOGRAPHY METHOD FOR RESOLVING HETEROGENIC ANTIBODY AGGREGATES |
| WO2013176754A1 (en) * | 2012-05-24 | 2013-11-28 | Abbvie Inc. | Novel purification of antibodies using hydrophobic interaction chromatography |
| ES2834113T3 (es) * | 2013-03-15 | 2021-06-16 | H Lundbeck As | Purificación de anticuerpos y monitorización de la pureza |
-
2014
- 2014-03-17 ES ES14763076T patent/ES2834113T3/es active Active
- 2014-03-17 TW TW103109933A patent/TWI670279B/zh active
- 2014-03-17 EP EP20188243.8A patent/EP3754012A1/en active Pending
- 2014-03-17 WO PCT/US2014/030558 patent/WO2014145744A1/en not_active Ceased
- 2014-03-17 TW TW108108923A patent/TW201940691A/zh unknown
- 2014-03-17 US US14/215,370 patent/US9518082B2/en active Active
- 2014-03-17 JP JP2016503420A patent/JP6472786B2/ja active Active
- 2014-03-17 AR ARP140101240A patent/AR095615A1/es unknown
- 2014-03-17 CA CA2905531A patent/CA2905531C/en active Active
- 2014-03-17 EP EP14763076.8A patent/EP2970865B1/en active Active
-
2016
- 2016-10-21 US US15/331,241 patent/US10533045B2/en active Active
-
2020
- 2020-01-10 US US16/739,724 patent/US20200223913A1/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2016512848A5 (enExample) | ||
| Chollangi et al. | Development of robust antibody purification by optimizing protein‐A chromatography in combination with precipitation methodologies | |
| TWI670279B (zh) | 抗體純化及純度監測 | |
| US11492372B2 (en) | Removal of leaked affinity purification ligand | |
| RU2019109975A (ru) | Способы очистки антител | |
| JP2017505620A5 (enExample) | ||
| EP3244738A1 (en) | Anti-glycoprotein antibodies and uses thereof | |
| WO2019152303A1 (en) | Systems and methods for characterizing drug product impurities | |
| CN106414495A (zh) | 抗体处理方法 | |
| KR20180125899A (ko) | 친화성 크로마토그래피를 이용한 항체 또는 항체절편 정제 방법 | |
| HK40042659A (en) | Antibody purification and purity monitoring | |
| WO2025219869A1 (en) | Methods of purifying immunoglobulins comprising n-terminal glutamine | |
| HK40072589A (en) | Removal of leaked affinity purification ligand | |
| KR20250025373A (ko) | 하류 항체 정제에서 uf/df 풀로부터의 집합체의 클리어런스 | |
| Chollangi et al. | ted |