AR095615A1 - Purificación de anticuerpos y monitoreo de pureza - Google Patents
Purificación de anticuerpos y monitoreo de purezaInfo
- Publication number
- AR095615A1 AR095615A1 ARP140101240A ARP140101240A AR095615A1 AR 095615 A1 AR095615 A1 AR 095615A1 AR P140101240 A ARP140101240 A AR P140101240A AR P140101240 A ARP140101240 A AR P140101240A AR 095615 A1 AR095615 A1 AR 095615A1
- Authority
- AR
- Argentina
- Prior art keywords
- purification
- proteins
- production
- impurities
- processes
- Prior art date
Links
- 238000000746 purification Methods 0.000 title abstract 4
- 238000012544 monitoring process Methods 0.000 title 1
- 239000012535 impurity Substances 0.000 abstract 4
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 3
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 3
- 238000004519 manufacturing process Methods 0.000 abstract 3
- 238000000034 method Methods 0.000 abstract 3
- 102000004169 proteins and genes Human genes 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 2
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 abstract 1
- 241000235058 Komagataella pastoris Species 0.000 abstract 1
- 230000002538 fungal effect Effects 0.000 abstract 1
- 229940067606 lecithin Drugs 0.000 abstract 1
- 235000010445 lecithin Nutrition 0.000 abstract 1
- 239000000787 lecithin Substances 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 229920001184 polypeptide Polymers 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 238000000159 protein binding assay Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/32—Bonded phase chromatography
- B01D15/325—Reversed phase
- B01D15/327—Reversed phase with hydrophobic interaction
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
- B01D15/3804—Affinity chromatography
- B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
- B01D15/424—Elution mode
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
- C07K2317/41—Glycosylation, sialylation, or fucosylation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/51—Complete heavy chain or Fd fragment, i.e. VH + CH1
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/515—Complete light chain, i.e. VL + CL
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/55—Fab or Fab'
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Water Supply & Treatment (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361792935P | 2013-03-15 | 2013-03-15 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR095615A1 true AR095615A1 (es) | 2015-10-28 |
Family
ID=51538079
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP140101240A AR095615A1 (es) | 2013-03-15 | 2014-03-17 | Purificación de anticuerpos y monitoreo de pureza |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US9518082B2 (enExample) |
| EP (2) | EP3754012A1 (enExample) |
| JP (1) | JP6472786B2 (enExample) |
| AR (1) | AR095615A1 (enExample) |
| CA (1) | CA2905531C (enExample) |
| ES (1) | ES2834113T3 (enExample) |
| TW (2) | TWI670279B (enExample) |
| WO (1) | WO2014145744A1 (enExample) |
Families Citing this family (53)
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| EP2702077A2 (en) | 2011-04-27 | 2014-03-05 | AbbVie Inc. | Methods for controlling the galactosylation profile of recombinantly-expressed proteins |
| WO2013158273A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Methods to modulate c-terminal lysine variant distribution |
| US9067990B2 (en) | 2013-03-14 | 2015-06-30 | Abbvie, Inc. | Protein purification using displacement chromatography |
| WO2013158279A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Protein purification methods to reduce acidic species |
| US9255154B2 (en) | 2012-05-08 | 2016-02-09 | Alderbio Holdings, Llc | Anti-PCSK9 antibodies and use thereof |
| US9512214B2 (en) | 2012-09-02 | 2016-12-06 | Abbvie, Inc. | Methods to control protein heterogeneity |
| CA2905010A1 (en) | 2013-03-12 | 2014-09-18 | Abbvie Inc. | Human antibodies that bind human tnf-alpha and methods of preparing the same |
| US10023608B1 (en) | 2013-03-13 | 2018-07-17 | Amgen Inc. | Protein purification methods to remove impurities |
| US9017687B1 (en) | 2013-10-18 | 2015-04-28 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same using displacement chromatography |
| WO2014151878A2 (en) | 2013-03-14 | 2014-09-25 | Abbvie Inc. | Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides |
| ES2834113T3 (es) * | 2013-03-15 | 2021-06-16 | H Lundbeck As | Purificación de anticuerpos y monitorización de la pureza |
| US9598667B2 (en) | 2013-10-04 | 2017-03-21 | Abbvie Inc. | Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins |
| US9181337B2 (en) | 2013-10-18 | 2015-11-10 | Abbvie, Inc. | Modulated lysine variant species compositions and methods for producing and using the same |
| US9085618B2 (en) | 2013-10-18 | 2015-07-21 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same |
| US20150139988A1 (en) | 2013-11-15 | 2015-05-21 | Abbvie, Inc. | Glycoengineered binding protein compositions |
| CA2954767A1 (en) * | 2014-07-14 | 2016-01-21 | Amgen Inc. | Crystalline antibody formulations |
| TW201628649A (zh) | 2014-10-09 | 2016-08-16 | 再生元醫藥公司 | 減少醫藥調配物中微可見顆粒之方法 |
| CN107148283A (zh) * | 2014-10-31 | 2017-09-08 | 豪夫迈·罗氏有限公司 | 抗il‑17a和il‑17f交叉反应性抗体变体、包含其的组合物及其制备和使用方法 |
| EP3334747B1 (en) | 2015-08-13 | 2023-09-27 | Amgen Inc. | Charged depth filtration of antigen-binding proteins |
| EP3344643B1 (en) | 2015-09-04 | 2025-07-02 | Qiagen Sciences LLC | Methods for co-isolation of nucleic acids and proteins |
| AU2017229635A1 (en) * | 2016-03-07 | 2018-09-20 | Hitachi Chemical Advanced Therapeutics Solutions, LLC | A closed system for labelling and selecting live cells |
| JP2019515671A (ja) | 2016-04-15 | 2019-06-13 | アルダー バイオファーマシューティカルズ、インコーポレイテッド | 抗ｐａｃａｐ抗体及びそれらの使用 |
| KR102369014B1 (ko) | 2016-08-16 | 2022-03-02 | 리제너론 파아마슈티컬스, 인크. | 혼합물로부터 개별 항체들을 정량하는 방법 |
| CN106371765B (zh) * | 2016-08-29 | 2020-09-18 | 成都科鸿达科技有限公司 | 一种高效大规模系统的ltl模型检测去内存抖动的方法 |
| CN106293544B (zh) * | 2016-08-29 | 2020-06-30 | 成都科鸿达科技有限公司 | 一种高效大规模系统的ltl模型检测方法 |
| ES3032778T3 (en) | 2016-10-25 | 2025-07-24 | Regeneron Pharma | Methods for chromatography data analysis |
| JP6829357B2 (ja) * | 2017-03-17 | 2021-02-10 | 住友ベークライト株式会社 | 抗体の糖鎖検出法 |
| CN107033236B (zh) * | 2017-05-05 | 2021-03-23 | 浙江大学 | 一种从酵母发酵液中分离人血白蛋白的混合模式层析方法 |
| CN107254502A (zh) * | 2017-07-26 | 2017-10-17 | 兰州名德农牧科技有限公司 | 一种动物蛋白酶制备羊胎盘肽的方法 |
| US11697670B2 (en) | 2017-08-22 | 2023-07-11 | Biogen Ma Inc. | Methods for purifying antibodies having reduced high molecular weight aggregates |
| MY203415A (en) | 2017-09-19 | 2024-06-27 | Regeneron Pharma | Methods of reducing particle formation and compositions formed thereby |
| US20210024873A1 (en) * | 2018-03-27 | 2021-01-28 | Bristol-Myers Squibb Company | Real-time monitoring of protein concentration using ultraviolet signal |
| US12018075B2 (en) | 2018-04-20 | 2024-06-25 | Janssen Biotech, Inc. | Chromatography column qualification in manufacturing methods for producing anti-TNF antibody compositions |
| AU2019260569B9 (en) | 2018-04-24 | 2025-12-11 | Qiagen Sciences Llc | Nucleic acid isolation and inhibitor removal from complex samples |
| GB201806752D0 (en) * | 2018-04-25 | 2018-06-06 | Ge Healthcare Bioprocess R&D Ab | Method in bioprocess system |
| TW202448568A (zh) | 2018-07-02 | 2024-12-16 | 美商里珍納龍藥品有限公司 | 自混合物製備多肽之系統及方法 |
| BR112021013734A2 (pt) | 2019-01-30 | 2021-09-21 | Regeneron Pharmaceuticals, Inc. | Método para caracterizar partículas visíveis e/ou subvisíveis em biofármacos |
| TW202532844A (zh) * | 2019-09-23 | 2025-08-16 | 美商健臻公司 | 用於測量生物樣品的分析物的多個產品品質屬性的方法 |
| CN111239408B (zh) * | 2020-01-19 | 2023-02-10 | 深圳格道糖生物技术有限公司 | 特定凝集素组合在构建基于唾液糖蛋白糖链鉴别肝纤维化的测试工具方面的应用 |
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| JP2020099909A (ja) * | 2020-03-17 | 2020-07-02 | ＨＯＹＡ Ｔｅｃｈｎｏｓｕｒｇｉｃａｌ株式会社 | 処理方法、生産方法およびハイドロキシアパタイト充填剤 |
| US20230167153A1 (en) * | 2020-05-01 | 2023-06-01 | Kashiv Biosciences, Llc | An improved process of purification of protein |
| CN111579777B (zh) * | 2020-05-29 | 2024-02-02 | 江苏省苏微微生物研究有限公司 | 一种以大粒径凝胶为载体的亲和介质及其应用 |
| AU2021381371A1 (en) * | 2020-11-18 | 2023-06-22 | Modernatx, Inc. | Ribonucleic acid purification |
| GB202105559D0 (en) * | 2021-04-19 | 2021-06-02 | Cytiva Bioprocess R & D Ab | Method of clarifying a crude protein solution |
| US20240383945A1 (en) * | 2021-05-10 | 2024-11-21 | Chiome Bioscience Inc. | Purification method of antibody composition |
| CN113416243B (zh) * | 2021-08-23 | 2021-12-10 | 上海盛迪医药有限公司 | 一种纯化抗体的方法 |
| CN114279968B (zh) * | 2021-12-28 | 2024-09-20 | 中国科学院长春应用化学研究所 | 一种电化学-双偏振干涉光电检测池 |
| CN116159340A (zh) * | 2022-12-16 | 2023-05-26 | 浙江大学 | 一种用于体积排阻色谱法的缓冲液 |
| WO2025155751A1 (en) * | 2024-01-17 | 2025-07-24 | Nutcracker Therapeutics, Inc. | Improved method for isolating and characterizing proteins |
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| EP2490780A4 (en) * | 2009-10-20 | 2014-04-09 | Merck Sharp & Dohme | USE OF A MIXED MODE CHROMATOGRAPHY FOR THE DETECTION AND PURIFICATION OF BASIC ANTIBODY PRODUCTS |
| SG185601A1 (en) * | 2010-05-18 | 2012-12-28 | Abbvie Inc | Apparatus and process of purification of proteins |
| US11214610B2 (en) | 2010-12-01 | 2022-01-04 | H. Lundbeck A/S | High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris |
| US9745373B2 (en) | 2011-05-20 | 2017-08-29 | Alderbio Holdings Llc | Anti-CGRP compositions and use thereof |
| WO2013013193A1 (en) * | 2011-07-20 | 2013-01-24 | Zepteon, Incorporated | Polypeptide separation methods |
| US10150968B2 (en) | 2011-08-19 | 2018-12-11 | Alderbio Holdings Llc | Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris |
| KR101978534B1 (ko) * | 2011-08-19 | 2019-08-28 | 앨더바이오 홀딩스 엘엘씨 | 변형된 미생물 이를 테면 피치아 파스토리스(pichia pastoris)에서 다수의 아단위 단백질들, 이를 테면 항체의 고역가 및 고순도 생산을 위한 다중-복사체 전략 |
| EP2773439A4 (en) * | 2011-10-31 | 2015-07-01 | Merck Sharp & Dohme | CHROMATOGRAPHY METHOD FOR RESOLVING HETEROGENIC ANTIBODY AGGREGATES |
| WO2013176754A1 (en) * | 2012-05-24 | 2013-11-28 | Abbvie Inc. | Novel purification of antibodies using hydrophobic interaction chromatography |
| ES2834113T3 (es) * | 2013-03-15 | 2021-06-16 | H Lundbeck As | Purificación de anticuerpos y monitorización de la pureza |
-
2014
- 2014-03-17 ES ES14763076T patent/ES2834113T3/es active Active
- 2014-03-17 TW TW103109933A patent/TWI670279B/zh active
- 2014-03-17 EP EP20188243.8A patent/EP3754012A1/en active Pending
- 2014-03-17 WO PCT/US2014/030558 patent/WO2014145744A1/en not_active Ceased
- 2014-03-17 TW TW108108923A patent/TW201940691A/zh unknown
- 2014-03-17 US US14/215,370 patent/US9518082B2/en active Active
- 2014-03-17 JP JP2016503420A patent/JP6472786B2/ja active Active
- 2014-03-17 AR ARP140101240A patent/AR095615A1/es unknown
- 2014-03-17 CA CA2905531A patent/CA2905531C/en active Active
- 2014-03-17 EP EP14763076.8A patent/EP2970865B1/en active Active
-
2016
- 2016-10-21 US US15/331,241 patent/US10533045B2/en active Active
-
2020
- 2020-01-10 US US16/739,724 patent/US20200223913A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CA2905531C (en) | 2023-01-03 |
| US20140288272A1 (en) | 2014-09-25 |
| EP3754012A1 (en) | 2020-12-23 |
| TW201514195A (zh) | 2015-04-16 |
| EP2970865B1 (en) | 2020-09-02 |
| TW201940691A (zh) | 2019-10-16 |
| EP2970865A1 (en) | 2016-01-20 |
| US9518082B2 (en) | 2016-12-13 |
| JP2016512848A (ja) | 2016-05-09 |
| JP6472786B2 (ja) | 2019-02-20 |
| WO2014145744A1 (en) | 2014-09-18 |
| CA2905531A1 (en) | 2014-09-18 |
| TWI670279B (zh) | 2019-09-01 |
| US10533045B2 (en) | 2020-01-14 |
| ES2834113T3 (es) | 2021-06-16 |
| US20200223913A1 (en) | 2020-07-16 |
| EP2970865A4 (en) | 2016-11-09 |
| US20170137500A1 (en) | 2017-05-18 |
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