JP2013511272A5 - - Google Patents
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- JP2013511272A5 JP2013511272A5 JP2012539436A JP2012539436A JP2013511272A5 JP 2013511272 A5 JP2013511272 A5 JP 2013511272A5 JP 2012539436 A JP2012539436 A JP 2012539436A JP 2012539436 A JP2012539436 A JP 2012539436A JP 2013511272 A5 JP2013511272 A5 JP 2013511272A5
- Authority
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- Prior art keywords
- glcnac
- cell
- nucleic acid
- acid encoding
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 210000004027 cell Anatomy 0.000 claims description 179
- 102000004169 proteins and genes Human genes 0.000 claims description 129
- 108090000623 proteins and genes Proteins 0.000 claims description 129
- 102000039446 nucleic acids Human genes 0.000 claims description 109
- 108020004707 nucleic acids Proteins 0.000 claims description 109
- 150000007523 nucleic acids Chemical class 0.000 claims description 109
- 238000000034 method Methods 0.000 claims description 102
- OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 claims description 66
- OVRNDRQMDRJTHS-RTRLPJTCSA-N N-acetyl-D-glucosamine Chemical compound CC(=O)N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-RTRLPJTCSA-N 0.000 claims description 66
- MBLBDJOUHNCFQT-LXGUWJNJSA-N N-acetylglucosamine Natural products CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 claims description 66
- 108060003306 Galactosyltransferase Proteins 0.000 claims description 59
- 102000030902 Galactosyltransferase Human genes 0.000 claims description 59
- 108010070113 alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I Proteins 0.000 claims description 45
- 230000008685 targeting Effects 0.000 claims description 42
- 101000918297 Caenorhabditis elegans Exostosin-2 homolog Proteins 0.000 claims description 38
- 239000002773 nucleotide Substances 0.000 claims description 32
- 125000003729 nucleotide group Chemical group 0.000 claims description 32
- 108010009689 mannosyl-oligosaccharide 1,2-alpha-mannosidase Proteins 0.000 claims description 26
- 102100021771 Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase Human genes 0.000 claims description 25
- 230000000694 effects Effects 0.000 claims description 24
- 108010083819 mannosyl-oligosaccharide 1,3 - 1,6-alpha-mannosidase Proteins 0.000 claims description 24
- GRHWEVYJIHXESA-HBHDJDHDSA-N beta-D-Galp-(1->4)-beta-D-GlcpNAc-(1->2)-alpha-D-Manp-(1->3)-[beta-D-Galp-(1->4)-beta-D-GlcpNAc-(1->2)-alpha-D-Manp-(1->6)]-beta-D-Manp-(1->4)-beta-D-GlcpNAc-(1->4)-beta-D-GlcpNAc Chemical compound O[C@@H]1[C@@H](NC(=O)C)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](NC(C)=O)[C@@H](O)[C@H](O[C@H]2[C@H]([C@@H](O[C@@H]3[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O[C@H]3[C@@H]([C@@H](O)[C@H](O[C@H]4[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O4)O)[C@@H](CO)O3)NC(C)=O)[C@H](O)[C@@H](CO[C@@H]3[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O[C@H]3[C@@H]([C@@H](O)[C@H](O[C@H]4[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O4)O)[C@@H](CO)O3)NC(C)=O)O2)O)[C@@H](CO)O1 GRHWEVYJIHXESA-HBHDJDHDSA-N 0.000 claims description 22
- 230000003834 intracellular effect Effects 0.000 claims description 22
- 241000235015 Yarrowia lipolytica Species 0.000 claims description 18
- WYUKJASPBYYQRJ-VSJOFRJTSA-N beta-D-GlcpNAc-(1->2)-alpha-D-Manp-(1->3)-[beta-D-GlcpNAc-(1->2)-alpha-D-Manp-(1->6)]-beta-D-Manp-(1->4)-beta-GlcpNAc-(1->4)-beta-D-GlcpNAc Chemical compound O[C@@H]1[C@@H](NC(=O)C)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](NC(C)=O)[C@@H](O)[C@H](O[C@H]2[C@H]([C@@H](O[C@@H]3[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)NC(C)=O)[C@H](O)[C@@H](CO[C@@H]3[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)NC(C)=O)O2)O)[C@@H](CO)O1 WYUKJASPBYYQRJ-VSJOFRJTSA-N 0.000 claims description 18
- 102000003886 Glycoproteins Human genes 0.000 claims description 17
- 108090000288 Glycoproteins Proteins 0.000 claims description 17
- 108010029607 4-nitrophenyl-alpha-glucosidase Proteins 0.000 claims description 16
- 210000002288 golgi apparatus Anatomy 0.000 claims description 16
- 101150061302 och1 gene Proteins 0.000 claims description 16
- 108010017836 1,3-alpha-D-glucan synthase Proteins 0.000 claims description 12
- 230000002950 deficient Effects 0.000 claims description 11
- 102100039059 Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase Human genes 0.000 claims description 8
- 101000958975 Homo sapiens Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase Proteins 0.000 claims description 8
- 210000002472 endoplasmic reticulum Anatomy 0.000 claims description 8
- 102000003951 Erythropoietin Human genes 0.000 claims description 6
- 108090000394 Erythropoietin Proteins 0.000 claims description 6
- 102000009109 Fc receptors Human genes 0.000 claims description 6
- 108010087819 Fc receptors Proteins 0.000 claims description 6
- 108010017213 Granulocyte-Macrophage Colony-Stimulating Factor Proteins 0.000 claims description 6
- 102000004457 Granulocyte-Macrophage Colony-Stimulating Factor Human genes 0.000 claims description 6
- 102000003996 Interferon-beta Human genes 0.000 claims description 6
- 108090000467 Interferon-beta Proteins 0.000 claims description 6
- 102000008070 Interferon-gamma Human genes 0.000 claims description 6
- 108010074328 Interferon-gamma Proteins 0.000 claims description 6
- 229940105423 erythropoietin Drugs 0.000 claims description 6
- 239000012634 fragment Substances 0.000 claims description 6
- 229960003130 interferon gamma Drugs 0.000 claims description 6
- 229960001388 interferon-beta Drugs 0.000 claims description 6
- 238000004519 manufacturing process Methods 0.000 claims description 6
- OXCMYAYHXIHQOA-UHFFFAOYSA-N potassium;[2-butyl-5-chloro-3-[[4-[2-(1,2,4-triaza-3-azanidacyclopenta-1,4-dien-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol Chemical compound [K+].CCCCC1=NC(Cl)=C(CO)N1CC1=CC=C(C=2C(=CC=CC=2)C2=N[N-]N=N2)C=C1 OXCMYAYHXIHQOA-UHFFFAOYSA-N 0.000 claims description 6
- 230000001225 therapeutic effect Effects 0.000 claims description 6
- INOZZBHURUDQQR-AJNGGQMLSA-N (2s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-2-amino-3-(1h-imidazol-5-yl)propanoyl]amino]-3-carboxypropanoyl]amino]-4-carboxybutanoyl]amino]-4-methylpentanoic acid Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](N)CC1=CN=CN1 INOZZBHURUDQQR-AJNGGQMLSA-N 0.000 claims description 4
- 102100032086 Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase Human genes 0.000 claims description 4
- 101000776319 Homo sapiens Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase Proteins 0.000 claims description 4
- 108010022308 beta-1,4-galactosyltransferase I Proteins 0.000 claims description 4
- 230000003197 catalytic effect Effects 0.000 claims description 4
- 230000004927 fusion Effects 0.000 claims description 4
- 108010041601 histidyl-aspartyl-glutamyl-leucine Proteins 0.000 claims description 4
- 208000002352 blister Diseases 0.000 claims 1
- 238000012217 deletion Methods 0.000 claims 1
- 230000037430 deletion Effects 0.000 claims 1
- 230000002538 fungal effect Effects 0.000 description 59
- 241000680806 Blastobotrys adeninivorans Species 0.000 description 3
- 108010054377 Mannosidases Proteins 0.000 description 2
- 102000001696 Mannosidases Human genes 0.000 description 2
- 101710163270 Nuclease Proteins 0.000 description 1
- 102000004357 Transferases Human genes 0.000 description 1
- 108090000992 Transferases Proteins 0.000 description 1
- 108010090473 UDP-N-acetylglucosamine-peptide beta-N-acetylglucosaminyltransferase Proteins 0.000 description 1
- 125000002519 galactosyl group Chemical group C1([C@H](O)[C@@H](O)[C@@H](O)[C@H](O1)CO)* 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US26282809P | 2009-11-19 | 2009-11-19 | |
| US61/262,828 | 2009-11-19 | ||
| PCT/IB2010/003154 WO2011061629A2 (en) | 2009-11-19 | 2010-11-19 | Yeast strains producing mammalian-like complex n-glycans |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2013511272A JP2013511272A (ja) | 2013-04-04 |
| JP2013511272A5 true JP2013511272A5 (enExample) | 2014-01-09 |
Family
ID=44060124
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012539436A Pending JP2013511272A (ja) | 2009-11-19 | 2010-11-19 | 哺乳類様複合ｎ−グリカンを生成する酵母株 |
Country Status (9)
| Country | Link |
|---|---|
| US (3) | US20130053550A1 (enExample) |
| EP (1) | EP2501805B1 (enExample) |
| JP (1) | JP2013511272A (enExample) |
| KR (1) | KR20130055555A (enExample) |
| CN (1) | CN102834509A (enExample) |
| BR (1) | BR112012011980A2 (enExample) |
| CA (1) | CA2781240A1 (enExample) |
| DK (1) | DK2501805T3 (enExample) |
| WO (1) | WO2011061629A2 (enExample) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2508613A3 (en) | 2007-04-03 | 2012-11-28 | Oxyrane UK Limited | Glycosylation of molecules |
| CN104651428A (zh) | 2009-09-29 | 2015-05-27 | 根特大学 | 水解甘露糖-1-磷酸-6-甘露糖连接为磷酸-6-甘露糖 |
| EP2501805B1 (en) | 2009-11-19 | 2019-02-20 | Oxyrane UK Limited | Yeast strains producing mammalian-like complex n-glycans |
| JP6151183B2 (ja) | 2010-09-29 | 2017-06-21 | オキシレイン ユーケー リミテッド | マンノース−１−ホスホ−６−マンノース結合からキャップを外すことおよびリン酸化ｎ−グリカンを脱マンノシル化することができるマンノシダーゼならびに哺乳動物細胞による糖タンパク質の取り込みを促進する方法 |
| CA2812872C (en) | 2010-09-29 | 2021-08-03 | Oxyrane Uk Limited | De-mannosylation of phosphorylated n-glycans |
| BR112014015933A2 (pt) | 2011-12-30 | 2019-09-24 | Oxyrane Uk Ltd | métodos e materiais para reduzir a degradação das proteínas recombinantes |
| US9567596B2 (en) * | 2012-01-05 | 2017-02-14 | Glykos Finland Oy | Protease deficient filamentous fungal cells and methods of use thereof |
| EP2825193B1 (en) | 2012-03-15 | 2019-11-13 | Oxyrane UK Limited | Methods and materials for treatment of pompe's disease |
| PL2991683T3 (pl) | 2013-05-02 | 2020-03-31 | Glykos Finland Oy | Koniugaty glikoproteiny lub glikanu z toksycznym ładunkiem |
| WO2015057064A1 (en) * | 2013-10-14 | 2015-04-23 | Synaffix B.V. | Modified glycoprotein, protein-conjugate and process for the preparation thereof |
| CN106574257B (zh) | 2014-02-24 | 2021-06-04 | 诺沃吉公司 | 二酰基甘油酰基转移酶(dga1)多核苷酸，和通过异源dga1的超量表达增加酵母细胞脂质生产的方法 |
| AU2015293949B2 (en) | 2014-07-21 | 2019-07-25 | Teknologian Tutkimuskeskus Vtt Oy | Production of glycoproteins with mammalian-like N-glycans in filamentous fungi |
| WO2017191208A1 (en) * | 2016-05-03 | 2017-11-09 | Vib Vzw | Means and methods for generating complex glycans derived from fungal engineered hosts |
| EP3784701A4 (en) | 2018-04-24 | 2022-06-08 | National Research Council of Canada | THERAPEUTIC COMPOUNDS CROSSING THE BLOOD-BRAIN BARRIER AND THEIR USES |
| WO2020060948A1 (en) | 2018-09-17 | 2020-03-26 | Levadura Biotechnology, Inc. | Production of cannabinoids in yeast using a fatty acid feedstock |
| KR102167388B1 (ko) * | 2020-01-15 | 2020-10-19 | 코스맥스 주식회사 | 신규 로도스포리디움 토룰로이데스 균주 및 그 균주 배양액을 포함하는 화장료 조성물 |
| CN116042562B (zh) * | 2022-03-11 | 2023-10-20 | 山东恒鲁生物科技有限公司 | 重组酵母菌及其应用 |
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| US7431927B2 (en) | 2005-03-24 | 2008-10-07 | Epitomics, Inc. | TNFα-neutralizing antibodies |
| CA2604917A1 (en) | 2005-04-14 | 2007-01-25 | Csir | Recombinant yeasts for synthesizing epoxide hydrolases |
| CN101313216A (zh) | 2005-09-22 | 2008-11-26 | 普洛茨股份有限公司 | 酵母突变体中产生的糖基化多肽及其使用方法 |
| US20080081035A1 (en) | 2006-10-03 | 2008-04-03 | National Enzyme Company | Therapeutic protease compositions |
| AU2008216418A1 (en) | 2007-02-09 | 2008-08-21 | Medimmune, Llc | Antibody libray display by yeast cell plasma membrane |
| EP2508613A3 (en) * | 2007-04-03 | 2012-11-28 | Oxyrane UK Limited | Glycosylation of molecules |
| US8569032B2 (en) | 2007-05-18 | 2013-10-29 | Tokyo Metropolitan Institute Of Medical Science | Proteins having acquired A-galactosidase activity |
| US8825012B2 (en) * | 2007-09-14 | 2014-09-02 | Unwired Planet, Llc | User data notification in a mobile communications system |
| SI3219795T1 (sl) | 2008-01-18 | 2024-10-30 | Biomarin Pharmaceutical Inc. | Izdelava akitvnih visoko fosforiliranih humanih encimov lizomske sulfataze in njihova uporaba |
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| CN102066422B (zh) | 2008-05-07 | 2015-07-22 | 宝玛瑞制药公司 | 溶酶体靶向肽及其应用 |
| CA2753584A1 (en) | 2009-02-26 | 2010-09-02 | Glaxosmithkline Llc | Host cells and methods of use |
| CN104651428A (zh) | 2009-09-29 | 2015-05-27 | 根特大学 | 水解甘露糖-1-磷酸-6-甘露糖连接为磷酸-6-甘露糖 |
| EP2501805B1 (en) | 2009-11-19 | 2019-02-20 | Oxyrane UK Limited | Yeast strains producing mammalian-like complex n-glycans |
| FR2954349A1 (fr) | 2009-12-22 | 2011-06-24 | Agronomique Inst Nat Rech | Sulfatase modifiant selectivement les glycosaminoglycanes |
| BR112012018163A2 (pt) | 2010-01-21 | 2019-09-24 | Oxyrane Uk Ltd | métodos e composições para exibição de um polipeptídeo sobre a superfície de uma célula de levredura |
| US20120135461A1 (en) | 2010-07-30 | 2012-05-31 | William James Cook | Production of glycoproteins with reduced o-glycosylation comprising the use of an alpha-1,2-mannosidase |
| CA2812872C (en) | 2010-09-29 | 2021-08-03 | Oxyrane Uk Limited | De-mannosylation of phosphorylated n-glycans |
| JP6151183B2 (ja) | 2010-09-29 | 2017-06-21 | オキシレイン ユーケー リミテッド | マンノース−１−ホスホ−６−マンノース結合からキャップを外すことおよびリン酸化ｎ−グリカンを脱マンノシル化することができるマンノシダーゼならびに哺乳動物細胞による糖タンパク質の取り込みを促進する方法 |
| BR112014015933A2 (pt) | 2011-12-30 | 2019-09-24 | Oxyrane Uk Ltd | métodos e materiais para reduzir a degradação das proteínas recombinantes |
| EP2825193B1 (en) | 2012-03-15 | 2019-11-13 | Oxyrane UK Limited | Methods and materials for treatment of pompe's disease |
| WO2014136065A2 (en) | 2013-03-05 | 2014-09-12 | Oxyrane Uk Limited | Production of catalytically active type i sulfatase |
-
2010
- 2010-11-19 EP EP10812879.4A patent/EP2501805B1/en active Active
- 2010-11-19 WO PCT/IB2010/003154 patent/WO2011061629A2/en not_active Ceased
- 2010-11-19 US US13/510,527 patent/US20130053550A1/en not_active Abandoned
- 2010-11-19 BR BR112012011980-0A patent/BR112012011980A2/pt not_active Application Discontinuation
- 2010-11-19 CA CA2781240A patent/CA2781240A1/en not_active Abandoned
- 2010-11-19 KR KR1020127015208A patent/KR20130055555A/ko not_active Withdrawn
- 2010-11-19 JP JP2012539436A patent/JP2013511272A/ja active Pending
- 2010-11-19 CN CN2010800618574A patent/CN102834509A/zh active Pending
- 2010-11-19 DK DK10812879.4T patent/DK2501805T3/en active
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2015
- 2015-03-06 US US14/641,002 patent/US10287557B2/en active Active
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2019
- 2019-04-11 US US16/381,523 patent/US11225646B2/en active Active
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