ATE229068T1 - Verfahren zur produktion von polypeptiden - Google Patents

Verfahren zur produktion von polypeptiden

Info

Publication number
ATE229068T1
ATE229068T1 AT97928141T AT97928141T ATE229068T1 AT E229068 T1 ATE229068 T1 AT E229068T1 AT 97928141 T AT97928141 T AT 97928141T AT 97928141 T AT97928141 T AT 97928141T AT E229068 T1 ATE229068 T1 AT E229068T1
Authority
AT
Austria
Prior art keywords
yeast cells
glucagon
genetically modified
short chain
polypeptides
Prior art date
Application number
AT97928141T
Other languages
English (en)
Inventor
Michi Egel-Mitani
Jakob Brandt
Knud Vad
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Application granted granted Critical
Publication of ATE229068T1 publication Critical patent/ATE229068T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/57509—Corticotropin releasing factor [CRF] (Urotensin)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/605—Glucagons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • Endocrinology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
AT97928141T 1996-07-05 1997-07-04 Verfahren zur produktion von polypeptiden ATE229068T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK74996 1996-07-05
PCT/DK1997/000298 WO1998001535A1 (en) 1996-07-05 1997-07-04 Method for the production of polypeptides

Publications (1)

Publication Number Publication Date
ATE229068T1 true ATE229068T1 (de) 2002-12-15

Family

ID=8097103

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97928141T ATE229068T1 (de) 1996-07-05 1997-07-04 Verfahren zur produktion von polypeptiden

Country Status (11)

Country Link
EP (1) EP0909312B1 (de)
JP (1) JP3998717B2 (de)
CN (1) CN1150313C (de)
AT (1) ATE229068T1 (de)
AU (1) AU3255497A (de)
CA (1) CA2258307C (de)
DE (1) DE69717629T2 (de)
DK (1) DK0909312T3 (de)
ES (1) ES2187782T3 (de)
IL (1) IL127583A0 (de)
WO (1) WO1998001535A1 (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5891669A (en) * 1997-03-17 1999-04-06 Novo Nordisk A/S, Novoalle, Methods for producing polypeptides in respiratory-deficient cells
AU2241699A (en) * 1998-01-27 1999-08-09 Board Of Regents Of The University And Community College System Of Nevada, The Expression of proteolytically-sensitive peptides
US6190883B1 (en) 1998-09-09 2001-02-20 Novo Nordisk A/S Method for the production of heterologous polypeptides in transformed yeast cells
US7371721B2 (en) 2000-09-18 2008-05-13 Sanos Bioscience A/S Use of GLP-2 and related compounds for the treatment, prevention, diagnosis, and prognosis of bone-related disorders and calcium homeostasis related syndromes
JP5161412B2 (ja) 2000-09-18 2013-03-13 サノス・バイオサイエンス・アクティーゼルスカブ Ｇｌｐ−１及びｇｌｐ−２ペプチドの使用方法
US7595172B2 (en) 2001-07-24 2009-09-29 Novo Nordisk A/S Method for making acylated polypeptides
KR100496758B1 (ko) 2002-06-29 2005-06-22 한국생명공학연구원 한세눌라 폴리모르파 앱신 유전자 결손 변이주 및 이를이용한 재조합 단백질 생산 방법
JP2007532096A (ja) 2003-11-14 2007-11-15 ノボ　ノルディスク　アクティーゼルスカブ アシル化されたインスリンの製造方法
CN102660614A (zh) 2005-08-16 2012-09-12 诺沃-诺迪斯克有限公司 制备成熟胰岛素多肽的方法
PL2038423T3 (pl) 2006-06-21 2013-05-31 Biocon Ltd Metoda wytwarzania biologicznie aktywnego polipeptydu insulinotropowego
WO2008037735A1 (en) 2006-09-27 2008-04-03 Novo Nordisk A/S Method for making maturated insulin polypeptides
EP2134853B1 (de) 2007-04-03 2018-07-18 Oxyrane UK Limited Glycosylierung von molekülen
KR101005821B1 (ko) * 2007-12-28 2011-01-05 (주)바이오버드 배트록소빈의 변이 뉴클레오타이드 서열, α－인자 분비 시그널 서열 변이체 및 이를 이용한 재조합 단백질의 제조방법및 이를 이용한 배트록소빈의 제조방법
CA2775938C (en) 2009-09-29 2021-08-10 Oxyrane Uk Limited Hydrolysis of mannose-1-phospho-6-mannose linkage to phospho-6-mannose
CN102834509A (zh) 2009-11-19 2012-12-19 奥克西雷恩英国有限公司 生成哺乳动物样复合n-聚糖的酵母菌株
US8815541B2 (en) * 2009-11-25 2014-08-26 Novo Nordisk A/S Method for making polypeptides
WO2012042386A2 (en) 2010-09-29 2012-04-05 Oxyrane Uk Limited Mannosidases capable of uncapping mannose-1-phospho-6-mannose linkages and demannosylating phosphorylated n-glycans and methods of facilitating mammalian cellular uptake of glycoproteins
EP2622089B1 (de) 2010-09-29 2023-11-01 Oxyrane UK Limited Demannosylierung von phosphorylierten n-glycanen
SG11201403506TA (en) * 2011-12-30 2014-07-30 Oxyrane Uk Ltd Methods and materials for reducing degradation of recombinant proteins
CA2867237C (en) 2012-03-15 2024-01-02 Wouter Vervecken Methods and materials for treatment of pompe's disease
AU2014225678B2 (en) * 2013-03-06 2016-11-17 Glaxosmithkline Llc Host cells and methods of use
CN106029688A (zh) * 2014-02-28 2016-10-12 诺和诺德股份有限公司 交配因子α原肽变体
US20220127324A1 (en) * 2019-02-24 2022-04-28 Oncosimis Biotech Private Limited Method for continuous production of recombinant glp-1 peptide by bacteria

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9404270D0 (en) * 1994-03-05 1994-04-20 Delta Biotechnology Ltd Yeast strains and modified albumins

Also Published As

Publication number Publication date
CN1225126A (zh) 1999-08-04
CA2258307C (en) 2008-02-12
DE69717629D1 (de) 2003-01-16
EP0909312B1 (de) 2002-12-04
CA2258307A1 (en) 1998-01-15
AU3255497A (en) 1998-02-02
WO1998001535A1 (en) 1998-01-15
IL127583A0 (en) 1999-10-28
ES2187782T3 (es) 2003-06-16
JP3998717B2 (ja) 2007-10-31
DK0909312T3 (da) 2003-03-17
DE69717629T2 (de) 2003-09-11
CN1150313C (zh) 2004-05-19
EP0909312A1 (de) 1999-04-21
JP2000513941A (ja) 2000-10-24

Similar Documents

Publication Publication Date Title
DE69717629D1 (de) Verfahren zur produktion von polypeptiden
DE68929115D1 (de) Verfahren zur Herstellung von menschlichem Serumalbumin in Hefe
ATE246246T1 (de) Herstellung von hämoglobin und analogen davon in nicht-erythrozytzellen
CA2155185A1 (en) Totally synthetic affinity reagents
CA2331337A1 (en) Site specific protein modification by mutagenesis
DE68929510D1 (de) Nukleotidsequenzen, die für ein Protein mit Ureaseaktivität kodieren
ATE443082T1 (de) Insulinvorstufen und verfahren zu deren herstellung
DE60028936D1 (de) Cyclosporin-derivate und verfahren zu deren herstellung
MX167236B (es) Peptidos sinteticos de timosina -1 y metodo para su obtencion
EP0297362A3 (de) Human-Aprotinin, dessen Lys-Rest in Position 15 gegen einen anderen protogenen Aminosäurerest ausgetauscht ist
DE3852105D1 (de) Verfahren zur herstellung von aprotinin und aprotinin-homologen in hefe.
DE3875359D1 (de) Dipeptide, verfahren zur herstellung und verwendung in der bestimmung von proteasen.
WO1998051780A3 (en) Method for increasing yields or recombinant proteins
EP0339217A3 (en) Specific peptide bond cleavage by organic tertiary phosphines with nucleophilic side chains
DE69019077D1 (de) Verfahren zur herstellung von c-terminalen alpha-amidierten peptiden.
ATE313632T1 (de) Durch pflanzen hergestellte rekombinante preduodenale lipase und abgeleitete polypeptide, verfahren zur herstellung und ihre verwendung
ATE171711T1 (de) Cyclohexapeptide und deren mischungen, verfahren zur herstellung sowie deren verwendung
AU637589B2 (en) Ancrod proteins, their production and use
ATE103341T1 (de) Antikoerper gegen hochkonservierte aminosaeuresequenzen von immunogenen substanzen, verfahren zur herstellung dieser antikoerper, sowie deren verwendung in immunoassays.
ATE141645T1 (de) In vitro-behandlung von fusionsproteinen
DE3781254D1 (de) Verfahren zur herstellung von eiweisshydrolysaten, loeslich in einem saeuremilieu und die erhaltenen hydrolysate.
DE3677851D1 (de) Verfahren zur steigerung der produktion von rekombinantem protein in hefearten der gattung saccharomyces.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties