DK0909312T3 - Fremgangsmåde til fremstilling af polypeptider - Google Patents

Fremgangsmåde til fremstilling af polypeptider

Info

Publication number
DK0909312T3
DK0909312T3 DK97928141T DK97928141T DK0909312T3 DK 0909312 T3 DK0909312 T3 DK 0909312T3 DK 97928141 T DK97928141 T DK 97928141T DK 97928141 T DK97928141 T DK 97928141T DK 0909312 T3 DK0909312 T3 DK 0909312T3
Authority
DK
Denmark
Prior art keywords
yeast cells
glucagon
genetically modified
short chain
polypeptides
Prior art date
Application number
DK97928141T
Other languages
English (en)
Inventor
Michi Egel-Mitani
Jakob Brandt
Knud Vad
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Application granted granted Critical
Publication of DK0909312T3 publication Critical patent/DK0909312T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/57509—Corticotropin releasing factor [CRF] (Urotensin)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/605—Glucagons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
DK97928141T 1996-07-05 1997-07-04 Fremgangsmåde til fremstilling af polypeptider DK0909312T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK74996 1996-07-05
PCT/DK1997/000298 WO1998001535A1 (en) 1996-07-05 1997-07-04 Method for the production of polypeptides

Publications (1)

Publication Number Publication Date
DK0909312T3 true DK0909312T3 (da) 2003-03-17

Family

ID=8097103

Family Applications (1)

Application Number Title Priority Date Filing Date
DK97928141T DK0909312T3 (da) 1996-07-05 1997-07-04 Fremgangsmåde til fremstilling af polypeptider

Country Status (11)

Country Link
EP (1) EP0909312B1 (da)
JP (1) JP3998717B2 (da)
CN (1) CN1150313C (da)
AT (1) ATE229068T1 (da)
AU (1) AU3255497A (da)
CA (1) CA2258307C (da)
DE (1) DE69717629T2 (da)
DK (1) DK0909312T3 (da)
ES (1) ES2187782T3 (da)
IL (1) IL127583A0 (da)
WO (1) WO1998001535A1 (da)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5891669A (en) * 1997-03-17 1999-04-06 Novo Nordisk A/S, Novoalle, Methods for producing polypeptides in respiratory-deficient cells
WO1999037758A2 (en) * 1998-01-27 1999-07-29 The Board Of Regents Of The University And Community College System Of Nevada On Behalf Of The University Of Nevada-Reno Expression of proteolytically-sensitive peptides
US6190883B1 (en) 1998-09-09 2001-02-20 Novo Nordisk A/S Method for the production of heterologous polypeptides in transformed yeast cells
DE60134251D1 (de) 2000-09-18 2008-07-10 Sanos Bioscience As Verwendung von glp-2-peptiden
US7371721B2 (en) 2000-09-18 2008-05-13 Sanos Bioscience A/S Use of GLP-2 and related compounds for the treatment, prevention, diagnosis, and prognosis of bone-related disorders and calcium homeostasis related syndromes
US7595172B2 (en) 2001-07-24 2009-09-29 Novo Nordisk A/S Method for making acylated polypeptides
ATE480624T1 (de) 2002-06-29 2010-09-15 Korea Res Inst Of Bioscience Yapsin-mangelmutantenstamm von hansenula polymorpha sowie verfahren zur herstellung rekombinanter proteine damit
JP2007532096A (ja) 2003-11-14 2007-11-15 ノボ　ノルディスク　アクティーゼルスカブ アシル化されたインスリンの製造方法
CN102660614A (zh) 2005-08-16 2012-09-12 诺沃-诺迪斯克有限公司 制备成熟胰岛素多肽的方法
US8114632B2 (en) 2006-06-21 2012-02-14 Biocon Limited Method of producing biologically active polypeptide having insulinotropic activity
EP2069502B1 (en) 2006-09-27 2014-02-26 Novo Nordisk A/S Method for making maturated insulin polypeptides
EP2134853B1 (en) 2007-04-03 2018-07-18 Oxyrane UK Limited Glycosylation of molecules
KR101005821B1 (ko) * 2007-12-28 2011-01-05 (주)바이오버드 배트록소빈의 변이 뉴클레오타이드 서열, α－인자 분비 시그널 서열 변이체 및 이를 이용한 재조합 단백질의 제조방법및 이를 이용한 배트록소빈의 제조방법
WO2011039634A2 (en) 2009-09-29 2011-04-07 Universiteit Gent Hydrolysis of mannose-1-phospho-6-mannose linkage to phospho-6-mannose
WO2011061629A2 (en) 2009-11-19 2011-05-26 Oxyrane Uk Limited Yeast strains producing mammalian-like complex n-glycans
EP2504355B1 (en) * 2009-11-25 2015-07-22 Novo Nordisk A/S Method for making polypeptides
US9347050B2 (en) 2010-09-29 2016-05-24 Oxyrane Uk Limited Mannosidases capable of uncapping mannose-1-phospho-6-mannose linkages and demannosylating phosphorylated N-glycans and methods of facilitating mammalian cellular uptake of glycoproteins
US9689015B2 (en) 2010-09-29 2017-06-27 Oxyrane Uk Limited De-mannosylation of phosphorylated N-glycans
JP2015503338A (ja) * 2011-12-30 2015-02-02 オキシレイン ユーケー リミテッド 組換えタンパク質の分解を低減させる方法および材料
EP3628326B1 (en) 2012-03-15 2024-02-28 Oxyrane UK Limited Methods and materials for treatment of pompe's disease
JP6742729B2 (ja) * 2013-03-06 2020-08-19 グラクソスミスクライン・リミテッド・ライアビリティ・カンパニーＧｌａｘｏＳｍｉｔｈＫｌｉｎｅ ＬＬＣ 宿主細胞および使用方法
US20160376330A1 (en) * 2014-02-28 2016-12-29 Novo Nordisk A/S Mating factor alpha pro-peptide variants
JP2022522276A (ja) * 2019-02-24 2022-04-15 オンコシミス バイオテック プライベート リミテッド 細菌による組換えｇｌｐ－１ペプチドの連続生産方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9404270D0 (en) * 1994-03-05 1994-04-20 Delta Biotechnology Ltd Yeast strains and modified albumins

Also Published As

Publication number Publication date
DE69717629D1 (de) 2003-01-16
JP2000513941A (ja) 2000-10-24
DE69717629T2 (de) 2003-09-11
CN1225126A (zh) 1999-08-04
ES2187782T3 (es) 2003-06-16
EP0909312A1 (en) 1999-04-21
WO1998001535A1 (en) 1998-01-15
AU3255497A (en) 1998-02-02
CA2258307A1 (en) 1998-01-15
IL127583A0 (en) 1999-10-28
CA2258307C (en) 2008-02-12
EP0909312B1 (en) 2002-12-04
CN1150313C (zh) 2004-05-19
JP3998717B2 (ja) 2007-10-31
ATE229068T1 (de) 2002-12-15

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