ES2187782T3 - Procedimiento para la produccion de polipeptidos. - Google Patents

Procedimiento para la produccion de polipeptidos.

Info

Publication number
ES2187782T3
ES2187782T3 ES97928141T ES97928141T ES2187782T3 ES 2187782 T3 ES2187782 T3 ES 2187782T3 ES 97928141 T ES97928141 T ES 97928141T ES 97928141 T ES97928141 T ES 97928141T ES 2187782 T3 ES2187782 T3 ES 2187782T3
Authority
ES
Spain
Prior art keywords
polypeptides
yeast cells
production
glucagon
genetically modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES97928141T
Other languages
English (en)
Inventor
Michi Egel-Mitani
Jakob Brandt
Knud Vad
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Application granted granted Critical
Publication of ES2187782T3 publication Critical patent/ES2187782T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/57509—Corticotropin releasing factor [CRF] (Urotensin)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/605—Glucagons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • Endocrinology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

LA PRESENTE INVENCION SE REFIERE A UN NUEVO PROCEDIMIENTO PARA LA OBTENCION DE POLIPEPTIDOS DE CADENA CORTA, INCLUIDOS LOS POLIPEPTIDOS QUE TIENEN HASTA 3 ENLACES DE DISULFURO Y/O ESTRUCTURAS RICAS EN RESIDUOS DE AMINOACIDOS BASICOS Y POLIPEPTIDOS DE CADENA CORTA ESTRUCTURADA ABIERTA, POR EJEMPLO, GLUCAGON, PEPTIDOS SIMILARES AL GLUCAGON Y SUS ANALOGOS FUNCIONALES, EN CELULAS DE LEVADURA GENETICAMENTE MODIFICADAS, QUE TIENEN UNA ACTIVIDAD REDUCIDA DE LA PROTEASA YAP3. LA INVENCION COMPRENDE ADEMAS CELULAS DE LEVADURA GENETICAMENTE MODIFICADAS, Y UN PROCEDIMIENTO PARA LA PREPARACION DE DICHAS CELULAS DE LEVADURA.
ES97928141T 1996-07-05 1997-07-04 Procedimiento para la produccion de polipeptidos. Expired - Lifetime ES2187782T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DK74996 1996-07-05

Publications (1)

Publication Number Publication Date
ES2187782T3 true ES2187782T3 (es) 2003-06-16

Family

ID=8097103

Family Applications (1)

Application Number Title Priority Date Filing Date
ES97928141T Expired - Lifetime ES2187782T3 (es) 1996-07-05 1997-07-04 Procedimiento para la produccion de polipeptidos.

Country Status (11)

Country Link
EP (1) EP0909312B1 (es)
JP (1) JP3998717B2 (es)
CN (1) CN1150313C (es)
AT (1) ATE229068T1 (es)
AU (1) AU3255497A (es)
CA (1) CA2258307C (es)
DE (1) DE69717629T2 (es)
DK (1) DK0909312T3 (es)
ES (1) ES2187782T3 (es)
IL (1) IL127583A0 (es)
WO (1) WO1998001535A1 (es)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5891669A (en) * 1997-03-17 1999-04-06 Novo Nordisk A/S, Novoalle, Methods for producing polypeptides in respiratory-deficient cells
AU2241699A (en) * 1998-01-27 1999-08-09 Board Of Regents Of The University And Community College System Of Nevada, The Expression of proteolytically-sensitive peptides
US6190883B1 (en) 1998-09-09 2001-02-20 Novo Nordisk A/S Method for the production of heterologous polypeptides in transformed yeast cells
WO2002022151A2 (en) 2000-09-18 2002-03-21 Osteometer Bio Tech A/S Use of glp-1 and flp-2 peptides for treatment of bone disorders
US7371721B2 (en) 2000-09-18 2008-05-13 Sanos Bioscience A/S Use of GLP-2 and related compounds for the treatment, prevention, diagnosis, and prognosis of bone-related disorders and calcium homeostasis related syndromes
US7595172B2 (en) 2001-07-24 2009-09-29 Novo Nordisk A/S Method for making acylated polypeptides
EP1534835B1 (en) 2002-06-29 2010-09-08 Korea Research Institute of Bioscience and Biotechnology Hansenula polymorpha yapsin deficient mutant strain and process for the preparation of recombinant proteins using the same
EP1687428A1 (en) 2003-11-14 2006-08-09 Novo Nordisk A/S Processes for making acylated insulin
EP1917363B1 (en) 2005-08-16 2011-06-22 Novo Nordisk A/S Method for making mature insulin polypeptides
CN101501209B (zh) 2006-06-21 2013-06-05 百奥勤有限公司 具有促胰岛素活性的生物活性多肽的制备方法
ES2464282T3 (es) 2006-09-27 2014-06-02 Novo Nordisk A/S Método para preparar polipéptidos de insulina madura
CN104480140B (zh) 2007-04-03 2019-12-31 奥克西雷恩英国有限公司 分子的糖基化
KR101005821B1 (ko) * 2007-12-28 2011-01-05 (주)바이오버드 배트록소빈의 변이 뉴클레오타이드 서열, α－인자 분비 시그널 서열 변이체 및 이를 이용한 재조합 단백질의 제조방법및 이를 이용한 배트록소빈의 제조방법
CN104651428A (zh) 2009-09-29 2015-05-27 根特大学 水解甘露糖-1-磷酸-6-甘露糖连接为磷酸-6-甘露糖
BR112012011980A2 (pt) 2009-11-19 2021-09-08 Oxyrane Uk Limited Métodos de produzir células transformadas de yarrowia lipolytica e proteínas-alvocompreendendo n-glicanos, células transformadas de yarrowia lipolytica e suas culturas, bem como composição compreendendo glicoproteínas
EP2504355B1 (en) * 2009-11-25 2015-07-22 Novo Nordisk A/S Method for making polypeptides
CN103328646B (zh) 2010-09-29 2021-06-18 奥克西雷恩英国有限公司 能够使甘露糖-1-磷酸-6-甘露糖连接脱帽并使磷酸化的n-聚糖脱甘露糖基化的甘露糖苷酶及促进糖蛋白的哺乳动物细胞摄取的方法
US9689015B2 (en) 2010-09-29 2017-06-27 Oxyrane Uk Limited De-mannosylation of phosphorylated N-glycans
WO2013098651A1 (en) * 2011-12-30 2013-07-04 Oxyrane Uk Limited Methods and materials for reducing degradation of recombinant proteins
US9249399B2 (en) 2012-03-15 2016-02-02 Oxyrane Uk Limited Methods and materials for treatment of pompe's disease
RU2663587C2 (ru) * 2013-03-06 2018-08-07 ГЛЭКСОСМИТКЛАЙН ЭлЭлСи Клетки-хозяева и способы использования
US20160376330A1 (en) * 2014-02-28 2016-12-29 Novo Nordisk A/S Mating factor alpha pro-peptide variants
KR20210138014A (ko) * 2019-02-24 2021-11-18 온코시미스 바이오테크 프라이빗 리미티드 박테리아에 의한 재조합 glp-1 펩타이드의 연속 생성 방법

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9404270D0 (en) * 1994-03-05 1994-04-20 Delta Biotechnology Ltd Yeast strains and modified albumins

Also Published As

Publication number Publication date
EP0909312A1 (en) 1999-04-21
EP0909312B1 (en) 2002-12-04
DE69717629D1 (de) 2003-01-16
CN1150313C (zh) 2004-05-19
IL127583A0 (en) 1999-10-28
CN1225126A (zh) 1999-08-04
JP3998717B2 (ja) 2007-10-31
WO1998001535A1 (en) 1998-01-15
DE69717629T2 (de) 2003-09-11
CA2258307C (en) 2008-02-12
JP2000513941A (ja) 2000-10-24
AU3255497A (en) 1998-02-02
DK0909312T3 (da) 2003-03-17
ATE229068T1 (de) 2002-12-15
CA2258307A1 (en) 1998-01-15

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