JP2014500019A5 - - Google Patents
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- Publication number
- JP2014500019A5 JP2014500019A5 JP2013540368A JP2013540368A JP2014500019A5 JP 2014500019 A5 JP2014500019 A5 JP 2014500019A5 JP 2013540368 A JP2013540368 A JP 2013540368A JP 2013540368 A JP2013540368 A JP 2013540368A JP 2014500019 A5 JP2014500019 A5 JP 2014500019A5
- Authority
- JP
- Japan
- Prior art keywords
- acetylglucosaminyltransferase
- catalytic domain
- recombinant protein
- domain
- chrysosporium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000003197 catalytic effect Effects 0.000 claims 25
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims 21
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims 21
- 241000123346 Chrysosporium Species 0.000 claims 14
- 239000002157 polynucleotide Substances 0.000 claims 12
- 102000040430 polynucleotide Human genes 0.000 claims 12
- 108091033319 polynucleotide Proteins 0.000 claims 12
- 108010070113 alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I Proteins 0.000 claims 10
- 108010073219 beta-1,3-galactosyl-0-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase Proteins 0.000 claims 10
- 230000002538 fungal effect Effects 0.000 claims 10
- 238000000034 method Methods 0.000 claims 10
- 108090000765 processed proteins & peptides Proteins 0.000 claims 8
- OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 claims 7
- MBLBDJOUHNCFQT-LXGUWJNJSA-N N-acetylglucosamine Natural products CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 claims 7
- 230000000694 effects Effects 0.000 claims 7
- 125000006850 spacer group Chemical group 0.000 claims 7
- 230000008685 targeting Effects 0.000 claims 7
- 241000223218 Fusarium Species 0.000 claims 6
- 101000829958 Homo sapiens N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase Proteins 0.000 claims 6
- 241001149964 Tolypocladium Species 0.000 claims 5
- 241001674013 Chrysosporium lucknowense Species 0.000 claims 4
- 241000226677 Myceliophthora Species 0.000 claims 4
- OVRNDRQMDRJTHS-RTRLPJTCSA-N N-acetyl-D-glucosamine Chemical compound CC(=O)N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-RTRLPJTCSA-N 0.000 claims 4
- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 claims 4
- 241000228341 Talaromyces Species 0.000 claims 4
- 241000223259 Trichoderma Species 0.000 claims 4
- 230000001086 cytosolic effect Effects 0.000 claims 4
- 229950006780 n-acetylglucosamine Drugs 0.000 claims 4
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 claims 3
- 241001019659 Acremonium <Plectosphaerellaceae> Species 0.000 claims 3
- 101150039297 Alg3 gene Proteins 0.000 claims 3
- 241000228212 Aspergillus Species 0.000 claims 3
- 241000223651 Aureobasidium Species 0.000 claims 3
- 241001337994 Cryptococcus <scale insect> Species 0.000 claims 3
- 241001344133 Magnaporthe Species 0.000 claims 3
- 241000235395 Mucor Species 0.000 claims 3
- 241000223251 Myrothecium Species 0.000 claims 3
- 241000233892 Neocallimastix Species 0.000 claims 3
- 241000221960 Neurospora Species 0.000 claims 3
- 241000228143 Penicillium Species 0.000 claims 3
- 241000235379 Piromyces Species 0.000 claims 3
- 241000222480 Schizophyllum Species 0.000 claims 3
- 241001494489 Thielavia Species 0.000 claims 3
- 108020001778 catalytic domains Proteins 0.000 claims 3
- 102000037865 fusion proteins Human genes 0.000 claims 3
- 108020001507 fusion proteins Proteins 0.000 claims 3
- 150000004676 glycans Chemical class 0.000 claims 3
- 101150061302 och1 gene Proteins 0.000 claims 3
- 108090000623 proteins and genes Proteins 0.000 claims 3
- 102000004169 proteins and genes Human genes 0.000 claims 3
- 102100021771 Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase Human genes 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 2
- 108010046068 N-Acetyllactosamine Synthase Proteins 0.000 claims 2
- 102000003838 Sialyltransferases Human genes 0.000 claims 2
- 108090000141 Sialyltransferases Proteins 0.000 claims 2
- 241001557886 Trichoderma sp. Species 0.000 claims 2
- 108010086873 UDP-N-acetylglucosamine transporter Proteins 0.000 claims 2
- 125000000539 amino acid group Chemical group 0.000 claims 2
- 238000012258 culturing Methods 0.000 claims 2
- 239000013604 expression vector Substances 0.000 claims 2
- 108010009689 mannosyl-oligosaccharide 1,2-alpha-mannosidase Proteins 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 101100184475 Candida albicans (strain SC5314 / ATCC MYA-2876) MNN24 gene Proteins 0.000 claims 1
- 101100130886 Candida albicans (strain SC5314 / ATCC MYA-2876) MNT1 gene Proteins 0.000 claims 1
- 108700023372 Glycosyltransferases Proteins 0.000 claims 1
- 102000051366 Glycosyltransferases Human genes 0.000 claims 1
- 102100021181 Golgi phosphoprotein 3 Human genes 0.000 claims 1
- 101001040734 Homo sapiens Golgi phosphoprotein 3 Proteins 0.000 claims 1
- 101001051674 Homo sapiens Meiosis-specific nuclear structural protein 1 Proteins 0.000 claims 1
- 241000223198 Humicola Species 0.000 claims 1
- 101100488151 Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) YEA4 gene Proteins 0.000 claims 1
- 101150093457 MNN2 gene Proteins 0.000 claims 1
- 101150075484 MNN9 gene Proteins 0.000 claims 1
- 102000001696 Mannosidases Human genes 0.000 claims 1
- 108010054377 Mannosidases Proteins 0.000 claims 1
- 108010087568 Mannosyltransferases Proteins 0.000 claims 1
- 102000006722 Mannosyltransferases Human genes 0.000 claims 1
- 102100024962 Meiosis-specific nuclear structural protein 1 Human genes 0.000 claims 1
- 102100023315 N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase Human genes 0.000 claims 1
- 108010056664 N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase Proteins 0.000 claims 1
- 101100454113 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) KRE2 gene Proteins 0.000 claims 1
- 101100181264 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) KTR6 gene Proteins 0.000 claims 1
- 101100457678 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) MNN10 gene Proteins 0.000 claims 1
- 101100184479 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) MNN4 gene Proteins 0.000 claims 1
- 101100406646 Schizosaccharomyces pombe (strain 972 / ATCC 24843) arg3 gene Proteins 0.000 claims 1
- 101150111043 VAN1 gene Proteins 0.000 claims 1
- 108010039255 alpha 1,6-mannosyltransferase Proteins 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 229920001184 polypeptide Polymers 0.000 claims 1
- 102000004196 processed proteins & peptides Human genes 0.000 claims 1
- 229910052705 radium Inorganic materials 0.000 claims 1
- HCWPIIXVSYCSAN-UHFFFAOYSA-N radium atom Chemical compound [Ra] HCWPIIXVSYCSAN-UHFFFAOYSA-N 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US41714410P | 2010-11-24 | 2010-11-24 | |
| US61/417,144 | 2010-11-24 | ||
| PCT/EP2011/070956 WO2012069593A2 (en) | 2010-11-24 | 2011-11-24 | Fusion enzymes |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014500019A JP2014500019A (ja) | 2014-01-09 |
| JP2014500019A5 true JP2014500019A5 (enExample) | 2015-01-08 |
| JP6017439B2 JP6017439B2 (ja) | 2016-11-02 |
Family
ID=45093734
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013540368A Expired - Fee Related JP6017439B2 (ja) | 2010-11-24 | 2011-11-24 | Ｎ−アセチルグルコサミニルトランスフェラーゼ活性を有する融合酵素 |
Country Status (14)
| Country | Link |
|---|---|
| US (2) | US9399764B2 (enExample) |
| EP (1) | EP2643456B1 (enExample) |
| JP (1) | JP6017439B2 (enExample) |
| KR (1) | KR20140018851A (enExample) |
| CN (1) | CN103348003B (enExample) |
| AU (1) | AU2011333685A1 (enExample) |
| BR (1) | BR112013013045A2 (enExample) |
| CA (1) | CA2819071A1 (enExample) |
| DK (1) | DK2643456T3 (enExample) |
| IL (1) | IL226481A0 (enExample) |
| MX (1) | MX338560B (enExample) |
| RU (1) | RU2013128619A (enExample) |
| SG (1) | SG190321A1 (enExample) |
| WO (1) | WO2012069593A2 (enExample) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2013128619A (ru) | 2010-11-24 | 2014-12-27 | Новартис Интернэшнл Фармасьютикал Лтд. | Слитные ферменты |
| DK2852610T3 (en) | 2012-05-23 | 2018-09-03 | Glykos Finland Oy | PRODUCTION OF FUCOSYLED GLYCOPROTEIN |
| CA2906671A1 (en) * | 2013-03-14 | 2014-09-25 | Glycobia, Inc. | Oligosaccharide compositions, glycoproteins and methods to produce the same in prokaryotes |
| WO2015001049A1 (en) | 2013-07-04 | 2015-01-08 | Novartis Ag | O-mannosyltransferase deficient filamentous fungal cells and methods of use thereof |
| PL3019621T3 (pl) | 2013-07-10 | 2019-05-31 | Glykos Finland Oy | Wytwarzanie glikoprotein o zwiększonym zajmowaniu miejsca N-glikozylacji |
| DK3019602T3 (en) * | 2013-07-10 | 2018-11-12 | Glykos Finland Oy | MULTIPLE PROTEASE-DEFECTED FILAMENTARY FUNGAL CELLS AND PROCEDURES FOR USE THEREOF |
| EP3027742B1 (en) | 2013-07-29 | 2019-11-20 | Danisco US Inc. | Variant enzymes |
| CA2954974A1 (en) | 2014-07-21 | 2016-01-28 | Glykos Finland Oy | Production of glycoproteins with mammalian-like n-glycans in filamentous fungi |
| CN105695439B (zh) * | 2014-11-27 | 2023-08-01 | 丰益(上海)生物技术研发中心有限公司 | 一种β-葡萄糖苷酶基因的重组表达方法 |
| AU2019325329A1 (en) * | 2018-08-21 | 2021-04-08 | Clara Foods Co. | Modification of protein glycosylation in microorganisms |
| KR102202606B1 (ko) * | 2018-11-30 | 2021-01-15 | (주)바이오스플래시 | 바이오레티놀을 생산하는 미생물 및 이를 이용한 바이오레티놀의 생산방법 |
| CN111699252B (zh) * | 2019-01-16 | 2024-04-30 | 福尼亚生物处理股份有限公司 | 内切葡聚糖酶组合物和方法 |
| CN110448719B (zh) * | 2019-06-28 | 2020-05-22 | 浙江大学 | 一种促进凝血的丝素-多肽电纺膜及其制备方法 |
| CN116121094B (zh) * | 2023-03-03 | 2024-07-12 | 天津科技大学 | 一株高产神经酰胺的酿酒酵母工程菌株、构建方法及其应用 |
Family Cites Families (31)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US4500707A (en) | 1980-02-29 | 1985-02-19 | University Patents, Inc. | Nucleosides useful in the preparation of polynucleotides |
| US4475196A (en) | 1981-03-06 | 1984-10-02 | Zor Clair G | Instrument for locating faults in aircraft passenger reading light and attendant call control system |
| US4447233A (en) | 1981-04-10 | 1984-05-08 | Parker-Hannifin Corporation | Medication infusion pump |
| US4439196A (en) | 1982-03-18 | 1984-03-27 | Merck & Co., Inc. | Osmotic drug delivery system |
| US4447224A (en) | 1982-09-20 | 1984-05-08 | Infusaid Corporation | Variable flow implantable infusion apparatus |
| US4487603A (en) | 1982-11-26 | 1984-12-11 | Cordis Corporation | Implantable microinfusion pump system |
| US4486194A (en) | 1983-06-08 | 1984-12-04 | James Ferrara | Therapeutic device for administering medicaments through the skin |
| US4743546A (en) | 1985-02-13 | 1988-05-10 | Biotechnica International, Inc. | Controlled gene excision |
| US4596556A (en) | 1985-03-25 | 1986-06-24 | Bioject, Inc. | Hypodermic injection apparatus |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4790824A (en) | 1987-06-19 | 1988-12-13 | Bioject, Inc. | Non-invasive hypodermic injection device |
| US4941880A (en) | 1987-06-19 | 1990-07-17 | Bioject, Inc. | Pre-filled ampule and non-invasive hypodermic injection device assembly |
| US5700637A (en) | 1988-05-03 | 1997-12-23 | Isis Innovation Limited | Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays |
| GB8822228D0 (en) | 1988-09-21 | 1988-10-26 | Southern E M | Support-bound oligonucleotides |
| US5312335A (en) | 1989-11-09 | 1994-05-17 | Bioject Inc. | Needleless hypodermic injection device |
| US5064413A (en) | 1989-11-09 | 1991-11-12 | Bioject, Inc. | Needleless hypodermic injection device |
| US5383851A (en) | 1992-07-24 | 1995-01-24 | Bioject Inc. | Needleless hypodermic injection device |
| DE19754622A1 (de) | 1997-12-09 | 1999-06-10 | Antje Von Dr Schaewen | Pflanzliche GntI-Sequenzen und Verwendung derselben zur Gewinnung von Pflanzen mit verminderter oder fehlender N-Acetylglucosaminyltransferase I (GnTI)-Aktivität |
| US7244601B2 (en) * | 1997-12-15 | 2007-07-17 | National Research Council Of Canada | Fusion proteins for use in enzymatic synthesis of oligosaccharides |
| CN1197965C (zh) | 1998-10-26 | 2005-04-20 | 诺维信公司 | 在丝状真菌细胞内构建和筛选目的dna文库 |
| CA2382184A1 (en) * | 1999-08-19 | 2001-03-01 | Kirin Beer Kabushiki Kaisha | Novel yeast mutants and process for producing glycoprotein containing mammalian type sugar chain |
| US7598055B2 (en) * | 2000-06-28 | 2009-10-06 | Glycofi, Inc. | N-acetylglucosaminyltransferase III expression in lower eukaryotes |
| US7795002B2 (en) | 2000-06-28 | 2010-09-14 | Glycofi, Inc. | Production of galactosylated glycoproteins in lower eukaryotes |
| KR101001243B1 (ko) | 2001-12-27 | 2010-12-17 | 글리코파이, 인크. | 포유동물-유형 탄수화물 구조의 설계 방법 |
| US7569376B2 (en) * | 2002-05-03 | 2009-08-04 | Neose Technologies, Inc. | Fucosyltransferase fusion protein |
| WO2006026992A1 (en) * | 2004-09-07 | 2006-03-16 | Novozymes A/S | Altered structure of n-glycans in a fungus |
| US7501275B2 (en) | 2004-10-27 | 2009-03-10 | The United States Of America As Represented By The Secretary Of Agriculture | Yeast transformation system |
| US20080026376A1 (en) | 2006-07-11 | 2008-01-31 | Huaming Wang | KEX2 cleavage regions of recombinant fusion proteins |
| CA2665120A1 (en) | 2006-10-03 | 2008-04-10 | Metabolic Explorer | Process for chromosomal integration and dna sequence replacement in clostridia |
| TW201028431A (en) * | 2008-10-31 | 2010-08-01 | Lonza Ag | Novel tools for the production of glycosylated proteins in host cells |
| RU2013128619A (ru) | 2010-11-24 | 2014-12-27 | Новартис Интернэшнл Фармасьютикал Лтд. | Слитные ферменты |
-
2011
- 2011-11-24 RU RU2013128619/10A patent/RU2013128619A/ru not_active Application Discontinuation
- 2011-11-24 EP EP11790938.2A patent/EP2643456B1/en not_active Not-in-force
- 2011-11-24 WO PCT/EP2011/070956 patent/WO2012069593A2/en not_active Ceased
- 2011-11-24 US US13/989,084 patent/US9399764B2/en active Active
- 2011-11-24 DK DK11790938.2T patent/DK2643456T3/en active
- 2011-11-24 CA CA2819071A patent/CA2819071A1/en not_active Abandoned
- 2011-11-24 KR KR1020137016445A patent/KR20140018851A/ko not_active Withdrawn
- 2011-11-24 BR BR112013013045-8A patent/BR112013013045A2/pt not_active IP Right Cessation
- 2011-11-24 JP JP2013540368A patent/JP6017439B2/ja not_active Expired - Fee Related
- 2011-11-24 CN CN201180064714.3A patent/CN103348003B/zh not_active Expired - Fee Related
- 2011-11-24 AU AU2011333685A patent/AU2011333685A1/en not_active Abandoned
- 2011-11-24 SG SG2013038088A patent/SG190321A1/en unknown
- 2011-11-24 MX MX2013005853A patent/MX338560B/es active IP Right Grant
-
2013
- 2013-05-21 IL IL226481A patent/IL226481A0/en unknown
-
2016
- 2016-07-08 US US15/205,577 patent/US20170002337A1/en not_active Abandoned
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