CN103348003B - 具有n-乙酰葡糖胺基转移酶活性的融合酶 - Google Patents

具有n-乙酰葡糖胺基转移酶活性的融合酶 Download PDF

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Publication number
CN103348003B
CN103348003B CN201180064714.3A CN201180064714A CN103348003B CN 103348003 B CN103348003 B CN 103348003B CN 201180064714 A CN201180064714 A CN 201180064714A CN 103348003 B CN103348003 B CN 103348003B
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catalytic domain
seq
recombinant protein
acetylglucosaminyltransferase
host cell
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Expired - Fee Related
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CN201180064714.3A
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Chinese (zh)
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CN103348003A (zh
Inventor
J·纳蒂南
A·康埃尔瓦
J·伊尔蒂南
M·萨洛埃莫
H·维斯卡里
A·于斯科南
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Glykos Finland Ltd
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Novartis International Pharmaceutical Ltd
Glykos Finland Ltd
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01101—Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (2.4.1.101)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01143—Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (2.4.1.143)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Life Sciences & Earth Sciences (AREA)
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  • Biochemistry (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201180064714.3A 2010-11-24 2011-11-24 具有n-乙酰葡糖胺基转移酶活性的融合酶 Expired - Fee Related CN103348003B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US41714410P 2010-11-24 2010-11-24
US61/417,144 2010-11-24
PCT/EP2011/070956 WO2012069593A2 (en) 2010-11-24 2011-11-24 Fusion enzymes

Publications (2)

Publication Number Publication Date
CN103348003A CN103348003A (zh) 2013-10-09
CN103348003B true CN103348003B (zh) 2016-09-07

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US (2) US9399764B2 (enExample)
EP (1) EP2643456B1 (enExample)
JP (1) JP6017439B2 (enExample)
KR (1) KR20140018851A (enExample)
CN (1) CN103348003B (enExample)
AU (1) AU2011333685A1 (enExample)
BR (1) BR112013013045A2 (enExample)
CA (1) CA2819071A1 (enExample)
DK (1) DK2643456T3 (enExample)
IL (1) IL226481A0 (enExample)
MX (1) MX338560B (enExample)
RU (1) RU2013128619A (enExample)
SG (1) SG190321A1 (enExample)
WO (1) WO2012069593A2 (enExample)

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DK2852610T3 (en) 2012-05-23 2018-09-03 Glykos Finland Oy PRODUCTION OF FUCOSYLED GLYCOPROTEIN
CA2906671A1 (en) * 2013-03-14 2014-09-25 Glycobia, Inc. Oligosaccharide compositions, glycoproteins and methods to produce the same in prokaryotes
WO2015001049A1 (en) 2013-07-04 2015-01-08 Novartis Ag O-mannosyltransferase deficient filamentous fungal cells and methods of use thereof
PL3019621T3 (pl) 2013-07-10 2019-05-31 Glykos Finland Oy Wytwarzanie glikoprotein o zwiększonym zajmowaniu miejsca N-glikozylacji
DK3019602T3 (en) * 2013-07-10 2018-11-12 Glykos Finland Oy MULTIPLE PROTEASE-DEFECTED FILAMENTARY FUNGAL CELLS AND PROCEDURES FOR USE THEREOF
EP3027742B1 (en) 2013-07-29 2019-11-20 Danisco US Inc. Variant enzymes
CA2954974A1 (en) 2014-07-21 2016-01-28 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
CN105695439B (zh) * 2014-11-27 2023-08-01 丰益(上海)生物技术研发中心有限公司 一种β-葡萄糖苷酶基因的重组表达方法
AU2019325329A1 (en) * 2018-08-21 2021-04-08 Clara Foods Co. Modification of protein glycosylation in microorganisms
KR102202606B1 (ko) * 2018-11-30 2021-01-15 (주)바이오스플래시 바이오레티놀을 생산하는 미생물 및 이를 이용한 바이오레티놀의 생산방법
CN111699252B (zh) * 2019-01-16 2024-04-30 福尼亚生物处理股份有限公司 内切葡聚糖酶组合物和方法
CN110448719B (zh) * 2019-06-28 2020-05-22 浙江大学 一种促进凝血的丝素-多肽电纺膜及其制备方法
CN116121094B (zh) * 2023-03-03 2024-07-12 天津科技大学 一株高产神经酰胺的酿酒酵母工程菌株、构建方法及其应用

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Publication number Publication date
KR20140018851A (ko) 2014-02-13
MX2013005853A (es) 2013-12-12
RU2013128619A (ru) 2014-12-27
US20170002337A1 (en) 2017-01-05
CN103348003A (zh) 2013-10-09
IL226481A0 (en) 2013-07-31
BR112013013045A2 (pt) 2021-10-26
CA2819071A1 (en) 2012-05-31
WO2012069593A2 (en) 2012-05-31
US9399764B2 (en) 2016-07-26
WO2012069593A3 (en) 2013-01-03
EP2643456B1 (en) 2016-05-11
EP2643456A2 (en) 2013-10-02
MX338560B (es) 2016-04-20
JP2014500019A (ja) 2014-01-09
DK2643456T3 (en) 2016-08-22
AU2011333685A1 (en) 2013-06-27
SG190321A1 (en) 2013-06-28
US20130330780A1 (en) 2013-12-12
JP6017439B2 (ja) 2016-11-02

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Effective date of registration: 20161017

Address after: Helsinki

Patentee after: Glykos Finland O. Y.

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Patentee before: Novartis Int Pharm Ltd.

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Granted publication date: 20160907

Termination date: 20181124