JP6017439B2 - N−アセチルグルコサミニルトランスフェラーゼ活性を有する融合酵素 - Google Patents

N−アセチルグルコサミニルトランスフェラーゼ活性を有する融合酵素 Download PDF

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JP6017439B2
JP6017439B2 JP2013540368A JP2013540368A JP6017439B2 JP 6017439 B2 JP6017439 B2 JP 6017439B2 JP 2013540368 A JP2013540368 A JP 2013540368A JP 2013540368 A JP2013540368 A JP 2013540368A JP 6017439 B2 JP6017439 B2 JP 6017439B2
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acetylglucosaminyltransferase
protein
glycan
gntii
catalytic domain
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JP2014500019A5 (enExample
JP2014500019A (ja
Inventor
ナトゥネン,ヤリ
カネルヴァ,アンネ
ヒルトゥネン,ユッカ
サロヘイモ,マルック
ヴィスカリ,ヘリ
フースコネン,アンネ
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Novartis International Pharmaceutical Ltd
Glykos Finland Ltd
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Novartis International Pharmaceutical Ltd
Glykos Finland Ltd
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1051Hexosyltransferases (2.4.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
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    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10Cells modified by introduction of foreign genetic material
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    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/18Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/005Glycopeptides, glycoproteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y204/00Glycosyltransferases (2.4)
    • C12Y204/01Hexosyltransferases (2.4.1)
    • C12Y204/01101Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (2.4.1.101)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y204/00Glycosyltransferases (2.4)
    • C12Y204/01Hexosyltransferases (2.4.1)
    • C12Y204/01143Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (2.4.1.143)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/50Improvements relating to the production of bulk chemicals
    • Y02P20/52Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Life Sciences & Earth Sciences (AREA)
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  • Wood Science & Technology (AREA)
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  • Physics & Mathematics (AREA)
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  • Cell Biology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2013540368A 2010-11-24 2011-11-24 N−アセチルグルコサミニルトランスフェラーゼ活性を有する融合酵素 Expired - Fee Related JP6017439B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US41714410P 2010-11-24 2010-11-24
US61/417,144 2010-11-24
PCT/EP2011/070956 WO2012069593A2 (en) 2010-11-24 2011-11-24 Fusion enzymes

Publications (3)

Publication Number Publication Date
JP2014500019A JP2014500019A (ja) 2014-01-09
JP2014500019A5 JP2014500019A5 (enExample) 2015-01-08
JP6017439B2 true JP6017439B2 (ja) 2016-11-02

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JP2013540368A Expired - Fee Related JP6017439B2 (ja) 2010-11-24 2011-11-24 N−アセチルグルコサミニルトランスフェラーゼ活性を有する融合酵素

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US (2) US9399764B2 (enExample)
EP (1) EP2643456B1 (enExample)
JP (1) JP6017439B2 (enExample)
KR (1) KR20140018851A (enExample)
CN (1) CN103348003B (enExample)
AU (1) AU2011333685A1 (enExample)
BR (1) BR112013013045A2 (enExample)
CA (1) CA2819071A1 (enExample)
DK (1) DK2643456T3 (enExample)
IL (1) IL226481A0 (enExample)
MX (1) MX338560B (enExample)
RU (1) RU2013128619A (enExample)
SG (1) SG190321A1 (enExample)
WO (1) WO2012069593A2 (enExample)

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DK2852610T3 (en) 2012-05-23 2018-09-03 Glykos Finland Oy PRODUCTION OF FUCOSYLED GLYCOPROTEIN
CA2906671A1 (en) * 2013-03-14 2014-09-25 Glycobia, Inc. Oligosaccharide compositions, glycoproteins and methods to produce the same in prokaryotes
WO2015001049A1 (en) 2013-07-04 2015-01-08 Novartis Ag O-mannosyltransferase deficient filamentous fungal cells and methods of use thereof
PL3019621T3 (pl) 2013-07-10 2019-05-31 Glykos Finland Oy Wytwarzanie glikoprotein o zwiększonym zajmowaniu miejsca N-glikozylacji
DK3019602T3 (en) * 2013-07-10 2018-11-12 Glykos Finland Oy MULTIPLE PROTEASE-DEFECTED FILAMENTARY FUNGAL CELLS AND PROCEDURES FOR USE THEREOF
EP3027742B1 (en) 2013-07-29 2019-11-20 Danisco US Inc. Variant enzymes
CA2954974A1 (en) 2014-07-21 2016-01-28 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
CN105695439B (zh) * 2014-11-27 2023-08-01 丰益(上海)生物技术研发中心有限公司 一种β-葡萄糖苷酶基因的重组表达方法
AU2019325329A1 (en) * 2018-08-21 2021-04-08 Clara Foods Co. Modification of protein glycosylation in microorganisms
KR102202606B1 (ko) * 2018-11-30 2021-01-15 (주)바이오스플래시 바이오레티놀을 생산하는 미생물 및 이를 이용한 바이오레티놀의 생산방법
CN111699252B (zh) * 2019-01-16 2024-04-30 福尼亚生物处理股份有限公司 内切葡聚糖酶组合物和方法
CN110448719B (zh) * 2019-06-28 2020-05-22 浙江大学 一种促进凝血的丝素-多肽电纺膜及其制备方法
CN116121094B (zh) * 2023-03-03 2024-07-12 天津科技大学 一株高产神经酰胺的酿酒酵母工程菌株、构建方法及其应用

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Also Published As

Publication number Publication date
KR20140018851A (ko) 2014-02-13
MX2013005853A (es) 2013-12-12
RU2013128619A (ru) 2014-12-27
US20170002337A1 (en) 2017-01-05
CN103348003A (zh) 2013-10-09
IL226481A0 (en) 2013-07-31
BR112013013045A2 (pt) 2021-10-26
CA2819071A1 (en) 2012-05-31
WO2012069593A2 (en) 2012-05-31
US9399764B2 (en) 2016-07-26
WO2012069593A3 (en) 2013-01-03
EP2643456B1 (en) 2016-05-11
CN103348003B (zh) 2016-09-07
EP2643456A2 (en) 2013-10-02
MX338560B (es) 2016-04-20
JP2014500019A (ja) 2014-01-09
DK2643456T3 (en) 2016-08-22
AU2011333685A1 (en) 2013-06-27
SG190321A1 (en) 2013-06-28
US20130330780A1 (en) 2013-12-12

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