MX338560B - Enzimas de fusion que tienen actividad de n-acetilglucosaminil transferasa. - Google Patents

Enzimas de fusion que tienen actividad de n-acetilglucosaminil transferasa.

Info

Publication number
MX338560B
MX338560B MX2013005853A MX2013005853A MX338560B MX 338560 B MX338560 B MX 338560B MX 2013005853 A MX2013005853 A MX 2013005853A MX 2013005853 A MX2013005853 A MX 2013005853A MX 338560 B MX338560 B MX 338560B
Authority
MX
Mexico
Prior art keywords
acetylglucosaminyltransferase activity
recombinant proteins
fusion enzymes
present disclosure
host cells
Prior art date
Application number
MX2013005853A
Other languages
English (en)
Other versions
MX2013005853A (es
Inventor
Markku Saloheimo
Anne Kanerva
Jari Natunen
Jukka Hiltunen
Heli Viskari
Anne Huuskonen
Original Assignee
Glykos Finland Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Glykos Finland Oy filed Critical Glykos Finland Oy
Publication of MX2013005853A publication Critical patent/MX2013005853A/es
Publication of MX338560B publication Critical patent/MX338560B/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01101—Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (2.4.1.101)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01143—Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (2.4.1.143)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente invención se refiere a proteínas recombinantes que tienen actividad de N-acetilglucosaminiltransferasa; la presente descripción se refiere adicionalmente a métodos para producir N-glicanos complejos que incluyen los pasos de proporcionar células hospederas que contienen dichas proteínas recombinantes y cultivar las células hospederas tal que las proteínas recombinantes se expresan.
MX2013005853A 2010-11-24 2011-11-24 Enzimas de fusion que tienen actividad de n-acetilglucosaminil transferasa. MX338560B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41714410P 2010-11-24 2010-11-24
PCT/EP2011/070956 WO2012069593A2 (en) 2010-11-24 2011-11-24 Fusion enzymes

Publications (2)

Publication Number Publication Date
MX2013005853A MX2013005853A (es) 2013-12-12
MX338560B true MX338560B (es) 2016-04-20

Family

ID=45093734

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013005853A MX338560B (es) 2010-11-24 2011-11-24 Enzimas de fusion que tienen actividad de n-acetilglucosaminil transferasa.

Country Status (14)

Country Link
US (2) US9399764B2 (es)
EP (1) EP2643456B1 (es)
JP (1) JP6017439B2 (es)
KR (1) KR20140018851A (es)
CN (1) CN103348003B (es)
AU (1) AU2011333685A1 (es)
BR (1) BR112013013045A2 (es)
CA (1) CA2819071A1 (es)
DK (1) DK2643456T3 (es)
IL (1) IL226481A0 (es)
MX (1) MX338560B (es)
RU (1) RU2013128619A (es)
SG (1) SG190321A1 (es)
WO (1) WO2012069593A2 (es)

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SG190321A1 (en) 2010-11-24 2013-06-28 Novartis Int Pharm Ltd Fusion enzymes having n-acetylglucosaminyltransferase activity
US9695454B2 (en) 2012-05-23 2017-07-04 Glykos Finland Oy Production of fucosylated glycoproteins
WO2014152137A2 (en) * 2013-03-14 2014-09-25 Glycobia, Inc. Oligosaccharide compositions, glycoproteins and methods to produce the same in prokaryotes
MX364532B (es) 2013-07-04 2019-04-29 Glykos Finland Oy Celulas de hongos filamentosos deficientes de o-manosiltransferasa y metodos de uso de las mismas.
KR20160035587A (ko) 2013-07-10 2016-03-31 노파르티스 아게 복수개의 프로테아제 결핍 사상형 진균 세포들 및 그의 이용방법
SG11201600098RA (en) 2013-07-10 2016-02-26 Novartis Ag Production of glycoproteins having increased n-glycosylation site occupancy
DK3027742T3 (da) 2013-07-29 2020-02-24 Danisco Us Inc Enzymvarianter
CN108064266A (zh) 2014-07-21 2018-05-22 格利科斯芬兰公司 在丝状真菌中具有哺乳动物样n-聚糖的糖蛋白的制备
CN105695439B (zh) * 2014-11-27 2023-08-01 丰益(上海)生物技术研发中心有限公司 一种β-葡萄糖苷酶基因的重组表达方法
JP2021534763A (ja) * 2018-08-21 2021-12-16 クララ フーズ カンパニー 微生物におけるタンパク質のグリコシル化の修飾
KR102202606B1 (ko) * 2018-11-30 2021-01-15 (주)바이오스플래시 바이오레티놀을 생산하는 미생물 및 이를 이용한 바이오레티놀의 생산방법
US10927358B2 (en) * 2019-01-16 2021-02-23 Fornia Biosolutions, Inc. Endoglucanase compositions and methods
CN110448719B (zh) * 2019-06-28 2020-05-22 浙江大学 一种促进凝血的丝素-多肽电纺膜及其制备方法
CN116121094B (zh) * 2023-03-03 2024-07-12 天津科技大学 一株高产神经酰胺的酿酒酵母工程菌株、构建方法及其应用
CN119876300B (zh) * 2025-03-28 2025-12-23 北京艾普希隆生物科技有限公司 一种制备唾液酸化普鲁兰多糖的方法

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Also Published As

Publication number Publication date
DK2643456T3 (en) 2016-08-22
WO2012069593A2 (en) 2012-05-31
JP6017439B2 (ja) 2016-11-02
EP2643456B1 (en) 2016-05-11
MX2013005853A (es) 2013-12-12
AU2011333685A1 (en) 2013-06-27
KR20140018851A (ko) 2014-02-13
RU2013128619A (ru) 2014-12-27
EP2643456A2 (en) 2013-10-02
IL226481A0 (en) 2013-07-31
CN103348003A (zh) 2013-10-09
CN103348003B (zh) 2016-09-07
WO2012069593A3 (en) 2013-01-03
US20170002337A1 (en) 2017-01-05
US20130330780A1 (en) 2013-12-12
US9399764B2 (en) 2016-07-26
JP2014500019A (ja) 2014-01-09
BR112013013045A2 (pt) 2021-10-26
CA2819071A1 (en) 2012-05-31
SG190321A1 (en) 2013-06-28

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