MX364532B - Celulas de hongos filamentosos deficientes de o-manosiltransferasa y metodos de uso de las mismas. - Google Patents

Celulas de hongos filamentosos deficientes de o-manosiltransferasa y metodos de uso de las mismas.

Info

Publication number
MX364532B
MX364532B MX2016000128A MX2016000128A MX364532B MX 364532 B MX364532 B MX 364532B MX 2016000128 A MX2016000128 A MX 2016000128A MX 2016000128 A MX2016000128 A MX 2016000128A MX 364532 B MX364532 B MX 364532B
Authority
MX
Mexico
Prior art keywords
filamentous fungal
mutation
mannosyltransferase
fungal cell
methods
Prior art date
Application number
MX2016000128A
Other languages
English (en)
Other versions
MX2016000128A (es
Inventor
Markku Saloheimo
Jari Natunen
Jukka Hiltunen
Anne Huuskonen
Ostermeier Christian
Patrick Sommer Benjamin
Wahl Ramon
Original Assignee
Glykos Finland Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=48740957&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX364532(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Glykos Finland Oy filed Critical Glykos Finland Oy
Publication of MX2016000128A publication Critical patent/MX2016000128A/es
Publication of MX364532B publication Critical patent/MX364532B/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01109—Dolichyl-phosphate-mannose-protein mannosyltransferase (2.4.1.109)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)

Abstract

La presente invención se refiere a una célula de hongo filamentoso deficiente de PMT, caracterizada porque comprende a) una primera mutación en un gen que codifica una proteasa endógena que reduce o elimina una actividad de proteasa endógena en comparación con una célula de hongo filamentoso parental que no tiene dicha primera mutación, dicha proteasa endógena se selecciona de entre proteasas aspárticas, proteasas de serina similar a tripsina, proteasas de subtilisina, proteasas glutámicas y proteasas de sedolisin, y b) una segunda mutación en un gen PMT que reduce la actividad endógena de O-manosiltransferasa en comparación con una célula de hongo filamentoso parental que no tiene dicha segunda mutación, en donde dicha célula de hongo filamentoso se selecciona del grupo que consiste de célula de Trichoderma, Neurospora, Myceliophthora y Chrysosporium.
MX2016000128A 2013-07-04 2014-07-03 Celulas de hongos filamentosos deficientes de o-manosiltransferasa y metodos de uso de las mismas. MX364532B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP13175141 2013-07-04
PCT/EP2014/064248 WO2015001049A1 (en) 2013-07-04 2014-07-03 O-mannosyltransferase deficient filamentous fungal cells and methods of use thereof

Publications (2)

Publication Number Publication Date
MX2016000128A MX2016000128A (es) 2016-03-01
MX364532B true MX364532B (es) 2019-04-29

Family

ID=48740957

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016000128A MX364532B (es) 2013-07-04 2014-07-03 Celulas de hongos filamentosos deficientes de o-manosiltransferasa y metodos de uso de las mismas.

Country Status (15)

Country Link
US (4) US20160376570A1 (es)
EP (1) EP3016970B1 (es)
KR (1) KR102111078B1 (es)
CN (1) CN105492457A (es)
BR (1) BR112015032695A2 (es)
CA (1) CA2916594C (es)
DK (1) DK3016970T3 (es)
ES (1) ES2739280T3 (es)
HR (1) HRP20191069T1 (es)
MX (1) MX364532B (es)
PL (1) PL3016970T3 (es)
RU (1) RU2016103373A (es)
SG (1) SG11201510814YA (es)
SI (1) SI3016970T1 (es)
WO (1) WO2015001049A1 (es)

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CN110885810B (zh) * 2019-12-16 2021-03-23 华中农业大学 一种利用真核发酵制备得到的抑菌蛋白酶CmYC1及其应用
CN115976033B (zh) * 2022-12-14 2024-05-17 西南大学 瓜实蝇tssk1和tssk3基因及其应用
KR20250004526A (ko) * 2023-06-29 2025-01-08 씨제이제일제당 (주) 아스파르트산 프로테아제 변이체
CN119931997B (zh) * 2025-04-09 2025-07-25 安琪酵母股份有限公司 天冬氨酸蛋白酶突变体及其应用

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Also Published As

Publication number Publication date
CN105492457A (zh) 2016-04-13
RU2016103373A (ru) 2017-08-10
EP3016970B1 (en) 2019-05-22
WO2015001049A1 (en) 2015-01-08
SI3016970T1 (sl) 2019-08-30
US20160376570A1 (en) 2016-12-29
HRP20191069T1 (hr) 2019-11-01
PL3016970T3 (pl) 2019-09-30
ES2739280T3 (es) 2020-01-30
RU2016103373A3 (es) 2018-03-26
US20200362319A1 (en) 2020-11-19
US20220213451A1 (en) 2022-07-07
US20200040314A1 (en) 2020-02-06
CA2916594A1 (en) 2015-01-08
SG11201510814YA (en) 2016-01-28
CA2916594C (en) 2020-03-10
BR112015032695A2 (pt) 2017-10-24
DK3016970T3 (da) 2019-06-24
US10724013B2 (en) 2020-07-28
KR102111078B1 (ko) 2020-05-18
KR20160030127A (ko) 2016-03-16
EP3016970A1 (en) 2016-05-11
MX2016000128A (es) 2016-03-01

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