JP2010519930A5 - - Google Patents

Info

Publication number

JP2010519930A5

JP2010519930A5 JP2009552704A JP2009552704A JP2010519930A5 JP 2010519930 A5 JP2010519930 A5 JP 2010519930A5 JP 2009552704 A JP2009552704 A JP 2009552704A JP 2009552704 A JP2009552704 A JP 2009552704A JP 2010519930 A5 JP2010519930 A5 JP 2010519930A5

Authority

JP

Japan

Prior art keywords

host cell

signal peptide

golgi

catalytic domain

recombinant glycoprotein

Prior art date

2008-03-03

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Pending

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• 210000004027 cell Anatomy 0.000 claims description 39
• 102000003886 Glycoproteins Human genes 0.000 claims description 23
• 108090000288 Glycoproteins Proteins 0.000 claims description 23
• 108010076504 Protein Sorting Signals Proteins 0.000 claims description 18
• 210000002288 golgi apparatus Anatomy 0.000 claims description 17
• 230000003197 catalytic effect Effects 0.000 claims description 16
• 230000000694 effects Effects 0.000 claims description 15
• 102100021771 Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase Human genes 0.000 claims description 6
• 108010009689 mannosyl-oligosaccharide 1,2-alpha-mannosidase Proteins 0.000 claims description 6
• 108090000992 Transferases Proteins 0.000 claims description 5
• 102000004357 Transferases Human genes 0.000 claims description 5
• 108010070113 alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I Proteins 0.000 claims description 5
• 229930182830 galactose Natural products 0.000 claims description 5
• 108010083819 mannosyl-oligosaccharide 1,3 - 1,6-alpha-mannosidase Proteins 0.000 claims description 5
• 101000918297 Caenorhabditis elegans Exostosin-2 homolog Proteins 0.000 claims description 4
• 102000003838 Sialyltransferases Human genes 0.000 claims description 4
• 108090000141 Sialyltransferases Proteins 0.000 claims description 4
• 108010039255 alpha 1,6-mannosyltransferase Proteins 0.000 claims description 2
• GRHWEVYJIHXESA-HBHDJDHDSA-N beta-D-Galp-(1->4)-beta-D-GlcpNAc-(1->2)-alpha-D-Manp-(1->3)-[beta-D-Galp-(1->4)-beta-D-GlcpNAc-(1->2)-alpha-D-Manp-(1->6)]-beta-D-Manp-(1->4)-beta-D-GlcpNAc-(1->4)-beta-D-GlcpNAc Chemical compound O[C@@H]1[C@@H](NC(=O)C)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](NC(C)=O)[C@@H](O)[C@H](O[C@H]2[C@H]([C@@H](O[C@@H]3[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O[C@H]3[C@@H]([C@@H](O)[C@H](O[C@H]4[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O4)O)[C@@H](CO)O3)NC(C)=O)[C@H](O)[C@@H](CO[C@@H]3[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O[C@H]3[C@@H]([C@@H](O)[C@H](O[C@H]4[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O4)O)[C@@H](CO)O3)NC(C)=O)O2)O)[C@@H](CO)O1 GRHWEVYJIHXESA-HBHDJDHDSA-N 0.000 claims description 2
• WYUKJASPBYYQRJ-VSJOFRJTSA-N beta-D-GlcpNAc-(1->2)-alpha-D-Manp-(1->3)-[beta-D-GlcpNAc-(1->2)-alpha-D-Manp-(1->6)]-beta-D-Manp-(1->4)-beta-GlcpNAc-(1->4)-beta-D-GlcpNAc Chemical compound O[C@@H]1[C@@H](NC(=O)C)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](NC(C)=O)[C@@H](O)[C@H](O[C@H]2[C@H]([C@@H](O[C@@H]3[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)NC(C)=O)[C@H](O)[C@@H](CO[C@@H]3[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)NC(C)=O)O2)O)[C@@H](CO)O1 WYUKJASPBYYQRJ-VSJOFRJTSA-N 0.000 claims description 2
• 108020001507 fusion proteins Proteins 0.000 claims 4
• 102000037865 fusion proteins Human genes 0.000 claims 4
• 230000037361 pathway Effects 0.000 claims 4
• 102000004190 Enzymes Human genes 0.000 claims 3
• 108090000790 Enzymes Proteins 0.000 claims 3
• 230000033581 fucosylation Effects 0.000 claims 3
• 108091026890 Coding region Proteins 0.000 claims 2
• 108020004414 DNA Proteins 0.000 claims 2
• 101000830049 Drosophila melanogaster GDP-mannose 4,6 dehydratase Proteins 0.000 claims 2
• 101710203794 GDP-fucose transporter Proteins 0.000 claims 2
• 230000001105 regulatory effect Effects 0.000 claims 2
• 230000008685 targeting Effects 0.000 claims 2
• 241001466460 Alveolata Species 0.000 claims 1
• 108020004705 Codon Proteins 0.000 claims 1
• 101001030665 Dictyostelium discoideum GDP-L-fucose synthase Proteins 0.000 claims 1
• 102000006471 Fucosyltransferases Human genes 0.000 claims 1
• 108010019236 Fucosyltransferases Proteins 0.000 claims 1
• 241000233866 Fungi Species 0.000 claims 1
• 241000235058 Komagataella pastoris Species 0.000 claims 1
• 241000243190 Microsporidia Species 0.000 claims 1
• 240000004808 Saccharomyces cerevisiae Species 0.000 claims 1
• 241001466451 Stramenopiles Species 0.000 claims 1
• 150000001413 amino acids Chemical class 0.000 claims 1
• 239000003054 catalyst Substances 0.000 claims 1
• 108020001778 catalytic domains Proteins 0.000 claims 1
• 238000005457 optimization Methods 0.000 claims 1
• HNQXDLYBFNWFEE-VHZSLYHRSA-N n-[(2r,3r,4r,5s,6r)-2-[(2r,3r,4s,5r)-2-acetamido-5-[(2r,3s,4s,5r,6r)-5-hydroxy-6-(hydroxymethyl)-3,4-bis[[(2r,3s,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy]oxan-2-yl]oxy-1-oxo-4-[(2s,3s,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] Chemical compound O([C@H]([C@H](C=O)NC(=O)C)[C@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H](CO[C@@H]1[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@@H]1[C@H]([C@@H](O[C@@H]2[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)[C@H](O)[C@@H](CO)O1)O[C@@H]1[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1NC(C)=O HNQXDLYBFNWFEE-VHZSLYHRSA-N 0.000 description 1
• 102000004169 proteins and genes Human genes 0.000 description 1
• 108090000623 proteins and genes Proteins 0.000 description 1