AU2008227024A1 - Production of glycoproteins with modified fucosylation - Google Patents

Production of glycoproteins with modified fucosylation Download PDF

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Publication number
AU2008227024A1
AU2008227024A1 AU2008227024A AU2008227024A AU2008227024A1 AU 2008227024 A1 AU2008227024 A1 AU 2008227024A1 AU 2008227024 A AU2008227024 A AU 2008227024A AU 2008227024 A AU2008227024 A AU 2008227024A AU 2008227024 A1 AU2008227024 A1 AU 2008227024A1
Authority
AU
Australia
Prior art keywords
host cell
glycoprotein
glycans
fucosylated
catalytic domain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2008227024A
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English (en)
Inventor
Stephen Hamilton
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Glycofi Inc
Original Assignee
Glycofi Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Glycofi Inc filed Critical Glycofi Inc
Publication of AU2008227024A1 publication Critical patent/AU2008227024A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2008227024A 2007-03-07 2008-03-03 Production of glycoproteins with modified fucosylation Abandoned AU2008227024A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US90534507P 2007-03-07 2007-03-07
US60/905,345 2007-03-07
PCT/US2008/002787 WO2008112092A2 (en) 2007-03-07 2008-03-03 Production of glycoproteins with modified fucosylation

Publications (1)

Publication Number Publication Date
AU2008227024A1 true AU2008227024A1 (en) 2008-09-18

Family

ID=39639063

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2008227024A Abandoned AU2008227024A1 (en) 2007-03-07 2008-03-03 Production of glycoproteins with modified fucosylation

Country Status (11)

Country Link
US (1) US20100028951A1 (enExample)
EP (1) EP2134834B1 (enExample)
JP (1) JP2010519930A (enExample)
KR (1) KR20090130029A (enExample)
CN (1) CN101679934B (enExample)
AU (1) AU2008227024A1 (enExample)
CA (1) CA2679732A1 (enExample)
RU (1) RU2479629C2 (enExample)
SG (1) SG187521A1 (enExample)
WO (1) WO2008112092A2 (enExample)
ZA (1) ZA200905728B (enExample)

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US20110300584A1 (en) * 2008-12-19 2011-12-08 Jennewein Biotechnologie Gmbh Synthesis of fucosylated compounds
CN102459603B (zh) 2009-06-02 2013-11-06 瑞泽恩制药公司 岩藻糖基化缺陷型细胞
CN102858949B (zh) 2010-02-24 2016-07-06 默沙东公司 用于增加在巴氏毕赤酵母中生产的治疗性糖蛋白上的n-糖基化位点占据的方法
TW201209160A (en) * 2010-04-27 2012-03-01 Lonza Ag Improved glycosylation of proteins in host cells
CA2827732A1 (en) 2011-02-25 2012-08-30 Merck Sharp & Dohme Corp. Yeast strain for the production of proteins with modified o-glycosylation
AU2012226398B9 (en) * 2011-03-06 2017-04-13 Merck Serono S.A. Low fucose cell lines and uses thereof
JP2014509864A (ja) 2011-03-23 2014-04-24 グリコド Ｇｄｐ−フコースを産生可能な酵母組換え細胞
US9695454B2 (en) 2012-05-23 2017-07-04 Glykos Finland Oy Production of fucosylated glycoproteins
TWI682033B (zh) * 2014-03-17 2020-01-11 泉盛生物科技股份有限公司 製造具有經修飾的糖苷化作用之重組糖蛋白之方法
CN108064266A (zh) 2014-07-21 2018-05-22 格利科斯芬兰公司 在丝状真菌中具有哺乳动物样n-聚糖的糖蛋白的制备
EP3042952A1 (en) 2015-01-07 2016-07-13 CEVEC Pharmaceuticals GmbH O-glycan sialylated recombinant glycoproteins and cell lines for producing the same
WO2017184562A1 (en) 2016-04-20 2017-10-26 Merck Sharp & Dohme Corp. Cmv neutralizing antigen binding proteins
EP3382014A1 (en) 2017-03-29 2018-10-03 CEVEC Pharmaceuticals GmbH Recombinant glycoproteins with reduced antennary fucosylation
EP3441471A1 (en) 2017-08-08 2019-02-13 CEVEC Pharmaceuticals GmbH Use of constitutively active variants of growth factor receptors as selection makers for the generation of stable producer cell lines
CN112672760B (zh) 2018-09-13 2025-05-30 德克萨斯大学体系董事会 新颖lilrb4抗体和其用途
JP7765397B2 (ja) 2020-03-12 2025-11-06 イミューン－オーエヌシー セラピューティクス，インコーポレーテッド 新規抗ｌｉｌｒｂ４抗体および派生産物
EP4623930A2 (en) 2020-12-03 2025-10-01 The Board Of Regents Of The University Of Texas System Methods for identifying lilrb-blocking antibodies
WO2025144785A2 (en) * 2023-12-28 2025-07-03 Dsm Ip Assets B.V. Cells and methods for the production of glycosylated compounds

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US5116964A (en) * 1989-02-23 1992-05-26 Genentech, Inc. Hybrid immunoglobulins
US5225538A (en) * 1989-02-23 1993-07-06 Genentech, Inc. Lymphocyte homing receptor/immunoglobulin fusion proteins
DE69434749T2 (de) * 1993-03-29 2007-04-26 Kyowa Hakko Kogyo Co., Ltd. Alpha-1,3-Fucosyltransferase
US20040191256A1 (en) * 1997-06-24 2004-09-30 Genentech, Inc. Methods and compositions for galactosylated glycoproteins
US20040136986A1 (en) * 1997-10-31 2004-07-15 Genentech, Inc. Methods and compositions comprising glycoprotein glycoforms
DK1071700T3 (da) * 1998-04-20 2010-06-07 Glycart Biotechnology Ag Glykosylerings-modifikation af antistoffer til forbedring af antistofafhængig cellulær cytotoksicitet
US20030175884A1 (en) * 2001-08-03 2003-09-18 Pablo Umana Antibody glycosylation variants having increased antibody-dependent cellular cytotoxicity
US6994966B2 (en) * 2000-02-17 2006-02-07 Glycominds Ltd. Combinatorial complex carbohydrate libraries and methods for the manufacture and uses thereof
AU3672800A (en) * 1999-04-09 2000-11-14 Kyowa Hakko Kogyo Co. Ltd. Method for controlling the activity of immunologically functional molecule
DE60139467D1 (de) * 2000-05-17 2009-09-17 Mitsubishi Tanabe Pharma Corp Verfahren zur herstellung von proteinen unter reduktion von mannosephosphat in der zuckerkette und dadurch hergestelltes glykoprotein
US7863020B2 (en) * 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
US7029872B2 (en) * 2000-06-28 2006-04-18 Glycofi, Inc Methods for producing modified glycoproteins
US8697394B2 (en) * 2000-06-28 2014-04-15 Glycofi, Inc. Production of modified glycoproteins having multiple antennary structures
US7795002B2 (en) * 2000-06-28 2010-09-14 Glycofi, Inc. Production of galactosylated glycoproteins in lower eukaryotes
US7598055B2 (en) * 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
US7449308B2 (en) * 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
US7625756B2 (en) * 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
US6946292B2 (en) * 2000-10-06 2005-09-20 Kyowa Hakko Kogyo Co., Ltd. Cells producing antibody compositions with increased antibody dependent cytotoxic activity
US20020132320A1 (en) * 2001-01-10 2002-09-19 Wang Peng George Glycoconjugate synthesis using a pathway-engineered organism
ATE430580T1 (de) * 2001-10-25 2009-05-15 Genentech Inc Glycoprotein-zusammensetzungen
US20040093621A1 (en) * 2001-12-25 2004-05-13 Kyowa Hakko Kogyo Co., Ltd Antibody composition which specifically binds to CD20
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EP1498491A4 (en) * 2002-04-09 2006-12-13 Kyowa Hakko Kogyo Kk METHOD FOR ENHANCING ACTIVITY OF BINDING ANTIBODY COMPOSITION WITH FC GAMMA IIIA RECEPTOR
PL222220B1 (pl) * 2003-01-22 2016-07-29 Glycart Biotechnology Ag Komórka gospodarza i sposób wytwarzania polipeptydu w komórce gospodarza
US7332299B2 (en) * 2003-02-20 2008-02-19 Glycofi, Inc. Endomannosidases in the modification of glycoproteins in eukaryotes
RU2250263C1 (ru) * 2003-08-05 2005-04-20 Федеральное государственное учреждение Всероссийский научно-исследовательский институт защиты животных (ФГУ ВНИИЗЖ) РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК p30NE2, КОДИРУЮЩАЯ 181-АМИНОКИСЛОТНЫЙ N-КОНЦЕВОЙ ФРАГМЕНТ ГЛИКОПРОТЕИНА E2 ВИРУСА КЛАССИЧЕСКОЙ ЧУМЫ СВИНЕЙ И ОБЕСПЕЧИВАЮЩАЯ ЕГО ЭКСПРЕССИЮ В КЛЕТКАХ БАКТЕРИЙ E.coli
EP1689348B1 (en) * 2003-12-05 2013-05-15 Children's Hospital Medical Center Oligosaccharide compositions and use thereof in the treatment of infection
CA2551484C (en) * 2003-12-24 2015-03-31 Glycofi, Inc. Methods for eliminating mannosylphosphorylation of glycans in the production of glycoproteins
JP4258662B2 (ja) * 2004-04-23 2009-04-30 独立行政法人産業技術総合研究所 Ｏ−フコース結合型タンパク質合成系遺伝子が導入された酵母形質転換体
AU2005238308B8 (en) * 2004-04-29 2009-10-08 Glycofi, Inc. Methods for reducing or eliminating alpha-mannosidase resistant glycans in the production of glycoproteins
KR20080021590A (ko) * 2005-03-24 2008-03-07 네오스 테크놀로지스, 인크. 가용성이고 활성인 진핵생물 글리코실트랜스퍼라제의원핵생물 유기체 내에서의 발현

Also Published As

Publication number Publication date
CN101679934B (zh) 2014-04-02
SG187521A1 (en) 2013-02-28
CN101679934A (zh) 2010-03-24
ZA200905728B (en) 2010-05-26
EP2134834B1 (en) 2013-04-17
RU2009136982A (ru) 2011-04-20
EP2134834A2 (en) 2009-12-23
KR20090130029A (ko) 2009-12-17
RU2479629C2 (ru) 2013-04-20
WO2008112092A2 (en) 2008-09-18
WO2008112092A3 (en) 2008-11-06
US20100028951A1 (en) 2010-02-04
JP2010519930A (ja) 2010-06-10
CA2679732A1 (en) 2008-09-18

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Legal Events

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MK4 Application lapsed section 142(2)(d) - no continuation fee paid for the application