JP2012135317A5 - - Google Patents
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- JP2012135317A5 JP2012135317A5 JP2012038487A JP2012038487A JP2012135317A5 JP 2012135317 A5 JP2012135317 A5 JP 2012135317A5 JP 2012038487 A JP2012038487 A JP 2012038487A JP 2012038487 A JP2012038487 A JP 2012038487A JP 2012135317 A5 JP2012135317 A5 JP 2012135317A5
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- acid
- acids
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- mixtures
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- 239000000203 mixture Substances 0.000 claims description 45
- 239000002253 acid Substances 0.000 claims description 20
- 230000006641 stabilisation Effects 0.000 claims description 18
- 238000011105 stabilization Methods 0.000 claims description 18
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 claims description 16
- 108020004707 nucleic acids Proteins 0.000 claims description 15
- 102000039446 nucleic acids Human genes 0.000 claims description 15
- 150000007523 nucleic acids Chemical class 0.000 claims description 15
- 150000001450 anions Chemical class 0.000 claims description 9
- 210000004369 blood Anatomy 0.000 claims description 9
- 239000008280 blood Substances 0.000 claims description 9
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 8
- -1 sulfuric acid alkali metal salt Chemical class 0.000 claims description 8
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 7
- 210000002381 plasma Anatomy 0.000 claims description 7
- 230000000087 stabilizing effect Effects 0.000 claims description 7
- OFOBLEOULBTSOW-UHFFFAOYSA-N Malonic acid Chemical compound OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 claims description 6
- 239000012472 biological sample Substances 0.000 claims description 5
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 5
- 108020004999 messenger RNA Proteins 0.000 claims description 5
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 4
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 150000007524 organic acids Chemical class 0.000 claims description 3
- 210000002966 serum Anatomy 0.000 claims description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims description 2
- 229910019142 PO4 Inorganic materials 0.000 claims description 2
- 150000003863 ammonium salts Chemical class 0.000 claims description 2
- 150000001767 cationic compounds Chemical class 0.000 claims description 2
- 235000005985 organic acids Nutrition 0.000 claims description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 2
- 239000010452 phosphate Substances 0.000 claims description 2
- 229920006395 saturated elastomer Polymers 0.000 claims description 2
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 claims description 2
- 150000007513 acids Chemical class 0.000 claims 10
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims 6
- 229920002477 rna polymer Polymers 0.000 claims 6
- 108020004414 DNA Proteins 0.000 claims 5
- 102000053602 DNA Human genes 0.000 claims 5
- 125000001931 aliphatic group Chemical group 0.000 claims 5
- 241000894006 Bacteria Species 0.000 claims 3
- 241000700605 Viruses Species 0.000 claims 3
- 229910052757 nitrogen Inorganic materials 0.000 claims 3
- 125000003837 (C1-C20) alkyl group Chemical group 0.000 claims 2
- 125000006736 (C6-C20) aryl group Chemical group 0.000 claims 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims 2
- WNLRTRBMVRJNCN-UHFFFAOYSA-N adipic acid Chemical compound OC(=O)CCCCC(O)=O WNLRTRBMVRJNCN-UHFFFAOYSA-N 0.000 claims 2
- 239000007864 aqueous solution Substances 0.000 claims 2
- 125000003710 aryl alkyl group Chemical group 0.000 claims 2
- 239000012620 biological material Substances 0.000 claims 2
- GHVNFZFCNZKVNT-UHFFFAOYSA-N decanoic acid Chemical compound CCCCCCCCCC(O)=O GHVNFZFCNZKVNT-UHFFFAOYSA-N 0.000 claims 2
- WWZKQHOCKIZLMA-UHFFFAOYSA-N octanoic acid Chemical compound CCCCCCCC(O)=O WWZKQHOCKIZLMA-UHFFFAOYSA-N 0.000 claims 2
- 229910052698 phosphorus Inorganic materials 0.000 claims 2
- 239000011574 phosphorus Substances 0.000 claims 2
- 150000007519 polyprotic acids Polymers 0.000 claims 2
- LDHQCZJRKDOVOX-UHFFFAOYSA-N trans-crotonic acid Natural products CC=CC(O)=O LDHQCZJRKDOVOX-UHFFFAOYSA-N 0.000 claims 2
- 150000003628 tricarboxylic acids Chemical class 0.000 claims 2
- RTBFRGCFXZNCOE-UHFFFAOYSA-N 1-methylsulfonylpiperidin-4-one Chemical compound CS(=O)(=O)N1CCC(=O)CC1 RTBFRGCFXZNCOE-UHFFFAOYSA-N 0.000 claims 1
- 108020000946 Bacterial DNA Proteins 0.000 claims 1
- CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 claims 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 claims 1
- 241000206602 Eukaryota Species 0.000 claims 1
- BDAGIHXWWSANSR-UHFFFAOYSA-M Formate Chemical compound [O-]C=O BDAGIHXWWSANSR-UHFFFAOYSA-M 0.000 claims 1
- OFOBLEOULBTSOW-UHFFFAOYSA-L Malonate Chemical compound [O-]C(=O)CC([O-])=O OFOBLEOULBTSOW-UHFFFAOYSA-L 0.000 claims 1
- CERQOIWHTDAKMF-UHFFFAOYSA-N Methacrylic acid Chemical compound CC(=C)C(O)=O CERQOIWHTDAKMF-UHFFFAOYSA-N 0.000 claims 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-N Succinic acid Natural products OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 claims 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims 1
- 108020004566 Transfer RNA Proteins 0.000 claims 1
- ATMLPEJAVWINOF-UHFFFAOYSA-N acrylic acid acrylic acid Chemical compound OC(=O)C=C.OC(=O)C=C ATMLPEJAVWINOF-UHFFFAOYSA-N 0.000 claims 1
- 239000001361 adipic acid Substances 0.000 claims 1
- 235000011037 adipic acid Nutrition 0.000 claims 1
- 229910052783 alkali metal Inorganic materials 0.000 claims 1
- 125000000217 alkyl group Chemical group 0.000 claims 1
- 150000001413 amino acids Chemical class 0.000 claims 1
- JFCQEDHGNNZCLN-UHFFFAOYSA-N anhydrous glutaric acid Natural products OC(=O)CCCC(O)=O JFCQEDHGNNZCLN-UHFFFAOYSA-N 0.000 claims 1
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 claims 1
- 125000004432 carbon atom Chemical group C* 0.000 claims 1
- LDHQCZJRKDOVOX-NSCUHMNNSA-N crotonic acid Chemical compound C\C=C\C(O)=O LDHQCZJRKDOVOX-NSCUHMNNSA-N 0.000 claims 1
- 150000001991 dicarboxylic acids Chemical class 0.000 claims 1
- WLGSIWNFEGRXDF-UHFFFAOYSA-N dodecanoic acid Chemical compound CCCCCCCCCCCC(O)=O.CCCCCCCCCCCC(O)=O WLGSIWNFEGRXDF-UHFFFAOYSA-N 0.000 claims 1
- LDHQCZJRKDOVOX-IHWYPQMZSA-N isocrotonic acid Chemical compound C\C=C/C(O)=O LDHQCZJRKDOVOX-IHWYPQMZSA-N 0.000 claims 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims 1
- 239000000178 monomer Substances 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 235000006408 oxalic acid Nutrition 0.000 claims 1
- 239000000546 pharmaceutical excipient Substances 0.000 claims 1
- 239000013612 plasmid Substances 0.000 claims 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims 1
- GBXQPDCOMJJCMJ-UHFFFAOYSA-M trimethyl-[6-(trimethylazaniumyl)hexyl]azanium;bromide Chemical compound [Br-].C[N+](C)(C)CCCCCC[N+](C)(C)C GBXQPDCOMJJCMJ-UHFFFAOYSA-M 0.000 claims 1
- 239000000052 vinegar Substances 0.000 claims 1
- 235000021419 vinegar Nutrition 0.000 claims 1
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 claims 1
- 229920002554 vinyl polymer Polymers 0.000 claims 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 18
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 13
- 239000000523 sample Substances 0.000 description 13
- 239000000654 additive Substances 0.000 description 12
- 239000000499 gel Substances 0.000 description 12
- 239000012528 membrane Substances 0.000 description 12
- 239000007993 MOPS buffer Substances 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 6
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 235000002906 tartaric acid Nutrition 0.000 description 6
- 239000011975 tartaric acid Substances 0.000 description 6
- 239000000872 buffer Substances 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 5
- 230000000996 additive effect Effects 0.000 description 4
- ROBFUDYVXSDBQM-UHFFFAOYSA-N hydroxymalonic acid Chemical compound OC(=O)C(O)C(O)=O ROBFUDYVXSDBQM-UHFFFAOYSA-N 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 239000011534 wash buffer Substances 0.000 description 4
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 3
- 235000011130 ammonium sulphate Nutrition 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 210000005084 renal tissue Anatomy 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 239000000377 silicon dioxide Substances 0.000 description 3
- 238000003260 vortexing Methods 0.000 description 3
- 229920000936 Agarose Polymers 0.000 description 2
- 238000010802 RNA extraction kit Methods 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 238000006911 enzymatic reaction Methods 0.000 description 2
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 2
- 150000007522 mineralic acids Chemical class 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 1
- 101150112014 Gapdh gene Proteins 0.000 description 1
- 108010074328 Interferon-gamma Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 230000021839 RNA stabilization Effects 0.000 description 1
- 208000005652 acute fatty liver of pregnancy Diseases 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 229910000318 alkali metal phosphate Inorganic materials 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 150000007942 carboxylates Chemical class 0.000 description 1
- 108091092328 cellular RNA Proteins 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 230000009395 genetic defect Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 125000001421 myristyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 1
- 238000012340 reverse transcriptase PCR Methods 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10031236.5 | 2000-06-27 | ||
| DE10031236A DE10031236A1 (de) | 2000-06-27 | 2000-06-27 | Verwendung von Carbonsäuren und anderen Additiven in Kombination mit kationischen Verbindungen zur Stabilisierung von Nukleinsäuren in biologischen Materialien |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002505349A Division JP5795455B2 (ja) | 2000-06-27 | 2001-05-22 | 生物材料中の核酸を安定化および／または単離するための新規な組成物 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2012135317A JP2012135317A (ja) | 2012-07-19 |
| JP2012135317A5 true JP2012135317A5 (enExample) | 2014-08-28 |
Family
ID=7646943
Family Applications (5)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002505349A Expired - Lifetime JP5795455B2 (ja) | 2000-06-27 | 2001-05-22 | 生物材料中の核酸を安定化および／または単離するための新規な組成物 |
| JP2002505350A Expired - Lifetime JP5657847B2 (ja) | 2000-06-27 | 2001-06-26 | 原核生物、菌類、原生動物もしくは藻類などの微生物中で、または微生物から核酸を安定化および／または単離するための、カチオン化合物およびプロトン供与体からなる組成物の使用 |
| JP2012038487A Pending JP2012135317A (ja) | 2000-06-27 | 2012-02-24 | 生物材料中の核酸を安定化および／または単離するための新規な組成物 |
| JP2014203545A Pending JP2015027310A (ja) | 2000-06-27 | 2014-10-02 | 原核生物、菌類、原生動物もしくは藻類などの微生物中で、または微生物から核酸を安定化および／または単離するための、カチオン化合物およびプロトン供与体からなる組成物の使用 |
| JP2015116549A Withdrawn JP2015212273A (ja) | 2000-06-27 | 2015-06-09 | 生物材料中の核酸を安定化および／または単離するための新規な組成物 |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002505349A Expired - Lifetime JP5795455B2 (ja) | 2000-06-27 | 2001-05-22 | 生物材料中の核酸を安定化および／または単離するための新規な組成物 |
| JP2002505350A Expired - Lifetime JP5657847B2 (ja) | 2000-06-27 | 2001-06-26 | 原核生物、菌類、原生動物もしくは藻類などの微生物中で、または微生物から核酸を安定化および／または単離するための、カチオン化合物およびプロトン供与体からなる組成物の使用 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014203545A Pending JP2015027310A (ja) | 2000-06-27 | 2014-10-02 | 原核生物、菌類、原生動物もしくは藻類などの微生物中で、または微生物から核酸を安定化および／または単離するための、カチオン化合物およびプロトン供与体からなる組成物の使用 |
| JP2015116549A Withdrawn JP2015212273A (ja) | 2000-06-27 | 2015-06-09 | 生物材料中の核酸を安定化および／または単離するための新規な組成物 |
Country Status (17)
| Country | Link |
|---|---|
| US (3) | US7270953B2 (enExample) |
| EP (3) | EP1820793B1 (enExample) |
| JP (5) | JP5795455B2 (enExample) |
| CN (1) | CN1250520C (enExample) |
| AT (3) | ATE374743T1 (enExample) |
| AU (4) | AU6903101A (enExample) |
| BR (1) | BR0112002A (enExample) |
| CA (2) | CA2412534C (enExample) |
| CZ (1) | CZ20024129A3 (enExample) |
| DE (3) | DE10031236A1 (enExample) |
| DK (2) | DK1294676T3 (enExample) |
| ES (2) | ES2295177T3 (enExample) |
| HU (1) | HUP0301342A2 (enExample) |
| MX (1) | MXPA02012261A (enExample) |
| PL (1) | PL360705A1 (enExample) |
| SK (1) | SK18022002A3 (enExample) |
| WO (2) | WO2002000599A1 (enExample) |
Families Citing this family (81)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7569342B2 (en) | 1997-12-10 | 2009-08-04 | Sierra Molecular Corp. | Removal of molecular assay interferences |
| US20080064108A1 (en) * | 1997-12-10 | 2008-03-13 | Tony Baker | Urine Preservation System |
| DE10031236A1 (de) * | 2000-06-27 | 2002-01-10 | Qiagen Gmbh | Verwendung von Carbonsäuren und anderen Additiven in Kombination mit kationischen Verbindungen zur Stabilisierung von Nukleinsäuren in biologischen Materialien |
| US6602718B1 (en) | 2000-11-08 | 2003-08-05 | Becton, Dickinson And Company | Method and device for collecting and stabilizing a biological sample |
| CA2428864C (en) * | 2000-11-08 | 2011-04-12 | Becton, Dickinson And Company | Method and device for collecting and stabilizing a biological sample |
| DE10147439B4 (de) * | 2001-09-26 | 2014-01-30 | Qiagen Gmbh | Verfahren zur Isolierung von DNA aus biologischen Proben |
| EP1329506A1 (en) * | 2002-01-18 | 2003-07-23 | Cypro S.A. | Method to quantify in vivo RNA levels |
| DE10208005A1 (de) * | 2002-02-26 | 2003-09-04 | Qiagen Gmbh | Verfahren zur Änderung der Transkriptkonzentration in ribonukleinsäurehaltigen biologischen Proben |
| US7482116B2 (en) | 2002-06-07 | 2009-01-27 | Dna Genotek Inc. | Compositions and methods for obtaining nucleic acids from sputum |
| WO2004020626A1 (de) * | 2002-08-09 | 2004-03-11 | Alexander Cherkasky | Verwendung von zellorganellen zur stabilisierung von biomolekülen und zellen |
| DE10336177B4 (de) * | 2002-08-09 | 2010-02-11 | Alexander Cherkasky | Verwendung von Zellorganellen zur Stabilisierung von Nukleinsäuren und Zellen |
| EP1581337A4 (en) * | 2002-10-18 | 2008-05-07 | Promega Corp | COMPOSITION FOR DISCONNECTING MOLECULES |
| JP2006528482A (ja) * | 2003-07-24 | 2006-12-21 | キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング | 核酸の逆転写及び／または増幅方法 |
| US20050059024A1 (en) | 2003-07-25 | 2005-03-17 | Ambion, Inc. | Methods and compositions for isolating small RNA molecules |
| GB0327319D0 (en) * | 2003-11-24 | 2003-12-24 | Pfizer Ltd | Novel pharmaceuticals |
| US20070196826A1 (en) * | 2004-03-23 | 2007-08-23 | Hiroyuki Ohno | Solvent For Dissolving Nucleic Acid, Nucleic Acid-Containing Solution And Method Of Preserving Nucleic Acid |
| US20080176209A1 (en) * | 2004-04-08 | 2008-07-24 | Biomatrica, Inc. | Integration of sample storage and sample management for life science |
| BRPI0509694A (pt) | 2004-04-08 | 2007-10-09 | Biomatrica Inc | integração de armazenamento de amostra e administração de amostra para ciências biológicas |
| US20060099567A1 (en) * | 2004-04-08 | 2006-05-11 | Biomatrica, Inc. | Integration of sample storage and sample management for life science |
| DE102004023417A1 (de) * | 2004-05-12 | 2005-12-08 | Clariant Gmbh | Verfahren zur Herstellung von langkettigen quaternären Ammonium-oxalaten und -hydrogenoxalaten |
| JP4810164B2 (ja) * | 2004-09-03 | 2011-11-09 | 富士フイルム株式会社 | 核酸分離精製方法 |
| US20060094023A1 (en) * | 2004-11-02 | 2006-05-04 | Industrial Technology Research Institute | Method for isolating nucleic acid by using amino surfactants |
| TWI294460B (en) * | 2004-12-23 | 2008-03-11 | Ind Tech Res Inst | Method for stabilizing nucleic acids |
| DE102005015005A1 (de) | 2005-04-01 | 2006-10-05 | Qiagen Gmbh | Verfahren zur Behandlung einer Biomoleküle enthaltenden Probe |
| US20060234251A1 (en) * | 2005-04-19 | 2006-10-19 | Lumigen, Inc. | Methods of enhancing isolation of RNA from biological samples |
| US20070015165A1 (en) * | 2005-07-13 | 2007-01-18 | Sigma-Aldrich Co. | Method for the isolation of RNA from biological sources |
| US20070190526A1 (en) * | 2006-02-16 | 2007-08-16 | Nexgen Diagnostics Llc | Methods of extracting nucleic acids |
| CN101448939B (zh) * | 2006-05-17 | 2011-12-14 | 小山义之 | 用于导入核酸、寡核酸或其衍生物的冷冻干燥体 |
| DE102007016707A1 (de) * | 2007-04-04 | 2008-10-09 | Qiagen Gmbh | Verfahren zur Aufreinigung von Biomolekülen |
| DE102007025276A1 (de) | 2007-05-31 | 2008-12-04 | Qiagen Gmbh | Aromatische Alkohole zur Behandlung einer biologischen Probe |
| DE102007025277A1 (de) | 2007-05-31 | 2008-12-04 | Qiagen Gmbh | Verfahren zur Stabilisierung einer biologischen Probe |
| DE102007025275A1 (de) | 2007-05-31 | 2008-12-04 | Qiagen Gmbh | Butendisäure oder deren Derivate zur Behandlung einer biologischen Probe |
| US20090053704A1 (en) * | 2007-08-24 | 2009-02-26 | Natalia Novoradovskaya | Stabilization of nucleic acids on solid supports |
| US7687027B2 (en) * | 2008-02-27 | 2010-03-30 | Becton, Dickinson And Company | Cleaning compositions, methods and materials for reducing nucleic acid contamination |
| AU2010227509A1 (en) | 2009-03-27 | 2011-09-08 | Universite Libre De Bruxelles | New marker for diagnosis of active multiple sclerosis |
| WO2011051402A1 (en) | 2009-11-02 | 2011-05-05 | Universite Libre De Bruxelles | New biomarkers for determining allergy status |
| EP2345719A1 (en) * | 2010-01-18 | 2011-07-20 | Qiagen GmbH | Method for isolating small RNA |
| US8932809B2 (en) * | 2010-01-19 | 2015-01-13 | Opgen, Inc. | Methods and kits for isolating nucleic acid from an organism |
| US20130095473A1 (en) | 2010-06-14 | 2013-04-18 | Qiagen Gmbh | Method for determination of target cells or tissue for extraction of biomolecules from fixed biological samples |
| EP2407540A1 (en) | 2010-07-15 | 2012-01-18 | Qiagen GmbH | Method for purifying a target nucleic acid |
| EP2598661B1 (en) | 2010-07-26 | 2017-09-27 | Biomatrica, INC. | Compositions for stabilizing dna, rna and proteins in saliva and other biological samples during shipping and storage at ambient temperatures |
| WO2012018638A2 (en) | 2010-07-26 | 2012-02-09 | Biomatrica, Inc. | Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures |
| CN102146422B (zh) * | 2011-01-24 | 2013-08-07 | 南京工业大学 | 一种丁二酸的发酵生产工艺 |
| DK3246416T3 (da) | 2011-04-15 | 2024-09-02 | Univ Johns Hopkins | Sikkert sekventeringssystem |
| MX347611B (es) | 2011-06-19 | 2017-05-04 | Abogen Inc | Dispositivos, soluciones y metodos para recoleccion de muestras. |
| EP3789500A1 (en) | 2011-08-12 | 2021-03-10 | QIAGEN GmbH | Method for isolating nucleic acids |
| ES2574956T3 (es) | 2011-09-26 | 2016-06-23 | Preanalytix Gmbh | Estabilización y aislamiento de ácidos nucleicos extracelulares |
| US11021733B2 (en) | 2011-09-26 | 2021-06-01 | Qiagen Gmbh | Stabilization and isolation of extracellular nucleic acids |
| AU2012314514B2 (en) | 2011-09-26 | 2018-03-15 | Qiagen Gmbh | Stabilisation and isolation of extracellular nucleic acids |
| CN104302783B (zh) | 2012-02-09 | 2018-02-09 | 生命技术公司 | 缀合的聚合物颗粒及其制备方法 |
| ES2567091T3 (es) | 2012-04-05 | 2016-04-19 | F. Hoffmann-La Roche Ag | Compuestos de amina para la preparación selectiva de muestras biológicas |
| US20150225712A1 (en) * | 2012-08-21 | 2015-08-13 | Qiagen Gmbh | Method for isolating nucleic acids from a formaldehyde releaser stabilized sample |
| US10233508B2 (en) | 2012-08-21 | 2019-03-19 | Qiagen Gmbh | Virus particle stabilisation and method for isolating viral nucleic acids |
| AU2013310861B2 (en) * | 2012-09-03 | 2019-02-14 | Qiagen Gmbh | Method for isolating RNA including small RNA with high yield |
| CA2884915C (en) | 2012-09-25 | 2022-05-17 | Qiagen Gmbh | Stabilisation of biological samples |
| ES2886507T5 (es) | 2012-10-29 | 2024-11-15 | Univ Johns Hopkins | Prueba de Papanicolaou para cánceres de ovario y de endometrio |
| EP3249054A1 (en) | 2012-12-20 | 2017-11-29 | Biomatrica, INC. | Formulations and methods for stabilizing pcr reagents |
| PL3745128T3 (pl) | 2012-12-28 | 2025-09-22 | Qiagen Sciences Llc | Test odpowiedzi immunologicznej pośredniczony przez komórki |
| GB201303666D0 (en) | 2013-03-01 | 2013-04-17 | Goldsborough Andrew S | Sample fixation and stabilisation |
| EP3447141B1 (en) | 2013-03-18 | 2020-08-05 | PreAnalytiX GmbH | Stabilisation of biological samples |
| WO2014146780A1 (en) | 2013-03-18 | 2014-09-25 | Qiagen Gmbh | Stabilization and isolation of extracellular nucleic acids |
| GB201305414D0 (en) | 2013-03-25 | 2013-05-08 | Arcis Biotechnology Holdings Ltd | Method and composition |
| RU2717644C2 (ru) | 2014-03-07 | 2020-03-24 | ДиЭнЭй ГЕНОТЕК ИНК. | Способ, композиция и набор для стабилизации дезоксирибонуклеиновых кислот в биологических образцах |
| US20160374330A1 (en) | 2014-03-18 | 2016-12-29 | Qiagen Gmbh | Stabilization and isolation of extracellular nucleic acids |
| EP3942931A1 (en) | 2014-06-10 | 2022-01-26 | Biomatrica, INC. | Stabilization of thrombocytes at ambient temperatures |
| CA2961613A1 (en) | 2014-09-17 | 2016-03-24 | Hologic, Inc. | Method of partial lysis and assay |
| ES2865280T3 (es) | 2015-05-27 | 2021-10-15 | Qiagen Gmbh | Composición y método para la disrupción de material de tejido |
| WO2017004559A1 (en) | 2015-07-02 | 2017-01-05 | Life Technologies Corporation | Conjugation of carboxyl functional hydrophilic beads |
| CN108350490B (zh) | 2015-07-06 | 2022-06-21 | 生命技术公司 | 用于测序的底物和方法 |
| WO2017027653A1 (en) | 2015-08-11 | 2017-02-16 | The Johns Hopkins University | Assaying ovarian cyst fluid |
| CA3005694A1 (en) | 2015-11-20 | 2017-05-26 | Qiagen Gmbh | Method of preparing sterilized compositions for stabilization of extracellular nucleic acids |
| KR20250047404A (ko) | 2015-12-08 | 2025-04-03 | 바이오매트리카 인코포레이티드 | 적혈구 침강 속도의 감소 |
| WO2018085928A1 (en) * | 2016-11-08 | 2018-05-17 | Qvella Corporation | Methods of performing nucleic acid stabilization and separation |
| SI3568475T1 (sl) | 2017-01-16 | 2023-05-31 | Spectrum Solutions L. L. C. | Raztopina za ohranjanje nukleinske kisline in načini uporabe |
| CN111868260B (zh) | 2017-08-07 | 2025-02-21 | 约翰斯霍普金斯大学 | 用于评估和治疗癌症的方法和材料 |
| CN110012899A (zh) * | 2019-04-12 | 2019-07-16 | 南京科佰生物科技有限公司 | 细胞用rna稳定剂及其使用方法 |
| CN115516108A (zh) | 2020-02-14 | 2022-12-23 | 约翰斯霍普金斯大学 | 评估核酸的方法和材料 |
| US20230203586A1 (en) * | 2020-05-29 | 2023-06-29 | The Rockefeller University | Method and system for rna isolation from self-collected and small volume samples |
| WO2023106190A1 (ja) * | 2021-12-10 | 2023-06-15 | 日東紡績株式会社 | 二本鎖核酸の融解温度上昇化剤及びその用途 |
| EP4446428A4 (en) | 2021-12-10 | 2025-04-23 | Nitto Boseki Co., Ltd. | Agent for increasing melting temperature (tm value) of nucleic acid |
| KR102786563B1 (ko) | 2023-04-27 | 2025-03-26 | 전남대학교산학협력단 | 수송배지로서 혈액 내의 핵산 보존용 조성물 및 이를 이용한 핵산의 보존방법 |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1289426A (enExample) * | 1970-06-22 | 1972-09-20 | ||
| US4900677A (en) * | 1986-09-26 | 1990-02-13 | E. I. Du Pont De Nemours And Company | Process for rapid isolation of high molecular weight DNA |
| US5010183A (en) | 1989-07-07 | 1991-04-23 | Macfarlane Donald E | Process for purifying DNA and RNA using cationic detergents |
| IT1240870B (it) | 1990-02-14 | 1993-12-17 | Talent | Procedimento per l'estrazione e la purificazione di dna genomico umano |
| US5196182A (en) | 1991-05-08 | 1993-03-23 | Streck Laboratories, Inc. | Tissue fixative |
| US5260048A (en) | 1991-05-08 | 1993-11-09 | Streck Laboratories, Inc. | Tissue fixative solution and method |
| US5275708A (en) * | 1992-03-20 | 1994-01-04 | Thomas Jefferson University | Cetyltrimethylammonium bromide gel electrophoresis |
| CA2107939C (en) * | 1993-01-13 | 2001-01-30 | Stephen B. Kong | Acidic aqueous cleaning compositions |
| US5300635A (en) | 1993-02-01 | 1994-04-05 | University Of Iowa Research Foundation | Quaternary amine surfactants and methods of using same in isolation of nucleic acids |
| AU6230594A (en) * | 1993-02-01 | 1994-08-29 | University Of Iowa Research Foundation, The | Quartenary amine surfactants and methods of using same in isolation of rna |
| US5300645A (en) * | 1993-04-14 | 1994-04-05 | Eli Lilly And Company | Tetrahydro-pyrido-indole |
| ZA943999B (en) * | 1993-06-09 | 1995-02-03 | Lonza Ag | Quaternary ammonium and waterproofing/preservative compositions |
| US5641726A (en) * | 1993-06-09 | 1997-06-24 | Lonza, Inc. | Quaternary ammonium carboxylate and borate compositions and preparation thereof |
| DK0743950T3 (da) | 1994-02-11 | 2002-03-25 | Qiagen Gmbh | Fremgangsmåde til adskillelse af dobbeltstrengede/enkeltstrengede nukleinsyre-strukturer |
| EP1038028A2 (en) | 1997-12-10 | 2000-09-27 | Sierra Diagnostics, Inc. | Methods and reagents for preservation of dna in bodily fluids |
| CN1283997A (zh) * | 1997-12-22 | 2001-02-14 | 人类基因组科学公司 | 角质形成细胞生长因子－2制剂 |
| US6204375B1 (en) * | 1998-07-31 | 2001-03-20 | Ambion, Inc. | Methods and reagents for preserving RNA in cell and tissue samples |
| US7683035B1 (en) | 1999-02-23 | 2010-03-23 | Qiagen, Gmbh | Method of stabilizing and/or isolating nucleic acids |
| DE10031236A1 (de) * | 2000-06-27 | 2002-01-10 | Qiagen Gmbh | Verwendung von Carbonsäuren und anderen Additiven in Kombination mit kationischen Verbindungen zur Stabilisierung von Nukleinsäuren in biologischen Materialien |
-
2000
- 2000-06-27 DE DE10031236A patent/DE10031236A1/de not_active Ceased
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- 2001-05-22 US US10/312,745 patent/US7270953B2/en not_active Expired - Lifetime
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- 2001-06-26 AT AT01953178T patent/ATE394363T1/de not_active IP Right Cessation
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- 2001-06-26 EP EP01953178A patent/EP1296932B1/de not_active Expired - Lifetime
- 2001-06-26 PL PL01360705A patent/PL360705A1/xx not_active Application Discontinuation
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- 2001-06-26 AU AU75685/01A patent/AU783922B2/en not_active Expired
- 2001-06-26 HU HU0301342A patent/HUP0301342A2/hu unknown
-
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- 2007-04-11 AU AU2007201583A patent/AU2007201583B2/en not_active Expired
- 2007-08-06 US US11/890,415 patent/US7682790B2/en not_active Expired - Fee Related
-
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- 2012-02-24 JP JP2012038487A patent/JP2012135317A/ja active Pending
-
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- 2014-10-02 JP JP2014203545A patent/JP2015027310A/ja active Pending
-
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- 2015-06-09 JP JP2015116549A patent/JP2015212273A/ja not_active Withdrawn
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