CN1250520C - 由阳离子化合物和质子供体组成的组合物的应用 - Google Patents

由阳离子化合物和质子供体组成的组合物的应用 Download PDF

Info

Publication number
CN1250520C
CN1250520C CNB01811945XA CN01811945A CN1250520C CN 1250520 C CN1250520 C CN 1250520C CN B01811945X A CNB01811945X A CN B01811945XA CN 01811945 A CN01811945 A CN 01811945A CN 1250520 C CN1250520 C CN 1250520C
Authority
CN
China
Prior art keywords
acid
composition according
nucleic acid
derived
acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CNB01811945XA
Other languages
English (en)
Chinese (zh)
Other versions
CN1438988A (zh
Inventor
尤维·奥尔米勒
坦杰·威尔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen GmbH
Original Assignee
Qiagen GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen GmbH filed Critical Qiagen GmbH
Publication of CN1438988A publication Critical patent/CN1438988A/zh
Application granted granted Critical
Publication of CN1250520C publication Critical patent/CN1250520C/zh
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C211/00—Compounds containing amino groups bound to a carbon skeleton
    • C07C211/62—Quaternary ammonium compounds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C211/00—Compounds containing amino groups bound to a carbon skeleton
    • C07C211/62—Quaternary ammonium compounds
    • C07C211/63—Quaternary ammonium compounds having quaternised nitrogen atoms bound to acyclic carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C211/00—Compounds containing amino groups bound to a carbon skeleton
    • C07C211/62—Quaternary ammonium compounds
    • C07C211/64—Quaternary ammonium compounds having quaternised nitrogen atoms bound to carbon atoms of six-membered aromatic rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/28—Phosphorus compounds with one or more P—C bonds
    • C07F9/54—Quaternary phosphonium compounds

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CNB01811945XA 2000-06-27 2001-06-26 由阳离子化合物和质子供体组成的组合物的应用 Expired - Lifetime CN1250520C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10031236.5 2000-06-27
DE10031236A DE10031236A1 (de) 2000-06-27 2000-06-27 Verwendung von Carbonsäuren und anderen Additiven in Kombination mit kationischen Verbindungen zur Stabilisierung von Nukleinsäuren in biologischen Materialien

Publications (2)

Publication Number Publication Date
CN1438988A CN1438988A (zh) 2003-08-27
CN1250520C true CN1250520C (zh) 2006-04-12

Family

ID=7646943

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB01811945XA Expired - Lifetime CN1250520C (zh) 2000-06-27 2001-06-26 由阳离子化合物和质子供体组成的组合物的应用

Country Status (17)

Country Link
US (3) US7270953B2 (enExample)
EP (3) EP1294676B1 (enExample)
JP (5) JP5795455B2 (enExample)
CN (1) CN1250520C (enExample)
AT (3) ATE524431T1 (enExample)
AU (4) AU6903101A (enExample)
BR (1) BR0112002A (enExample)
CA (2) CA2412534C (enExample)
CZ (1) CZ20024129A3 (enExample)
DE (3) DE10031236A1 (enExample)
DK (2) DK1294676T3 (enExample)
ES (2) ES2295177T3 (enExample)
HU (1) HUP0301342A2 (enExample)
MX (1) MXPA02012261A (enExample)
PL (1) PL360705A1 (enExample)
SK (1) SK18022002A3 (enExample)
WO (2) WO2002000599A1 (enExample)

Families Citing this family (82)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080064108A1 (en) * 1997-12-10 2008-03-13 Tony Baker Urine Preservation System
US7569342B2 (en) 1997-12-10 2009-08-04 Sierra Molecular Corp. Removal of molecular assay interferences
DE10031236A1 (de) * 2000-06-27 2002-01-10 Qiagen Gmbh Verwendung von Carbonsäuren und anderen Additiven in Kombination mit kationischen Verbindungen zur Stabilisierung von Nukleinsäuren in biologischen Materialien
US6602718B1 (en) 2000-11-08 2003-08-05 Becton, Dickinson And Company Method and device for collecting and stabilizing a biological sample
JP5192631B2 (ja) * 2000-11-08 2013-05-08 ベクトン・ディキンソン・アンド・カンパニー 生物学的試料を収集し安定化させるための方法および装置
DE10147439B4 (de) * 2001-09-26 2014-01-30 Qiagen Gmbh Verfahren zur Isolierung von DNA aus biologischen Proben
EP1329506A1 (en) 2002-01-18 2003-07-23 Cypro S.A. Method to quantify in vivo RNA levels
DE10208005A1 (de) * 2002-02-26 2003-09-04 Qiagen Gmbh Verfahren zur Änderung der Transkriptkonzentration in ribonukleinsäurehaltigen biologischen Proben
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
DE10336177B4 (de) * 2002-08-09 2010-02-11 Alexander Cherkasky Verwendung von Zellorganellen zur Stabilisierung von Nukleinsäuren und Zellen
WO2004020626A1 (de) * 2002-08-09 2004-03-11 Alexander Cherkasky Verwendung von zellorganellen zur stabilisierung von biomolekülen und zellen
AU2003286553A1 (en) * 2002-10-18 2004-05-04 Promega Corporation Methods for separating molecules
WO2005010209A2 (de) * 2003-07-24 2005-02-03 Qiagen Gmbh Verfahren zur reversen transkription und/oder amplifikation von nukleinsäuren
US20050059024A1 (en) * 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
GB0327319D0 (en) * 2003-11-24 2003-12-24 Pfizer Ltd Novel pharmaceuticals
EP1736542A4 (en) * 2004-03-23 2008-10-29 Hiroyuki Ohno SOLVENTS FOR SOLVING NUCLEIC ACID, NUCLEIC ACIDIC SOLUTION, AND METHOD FOR PRESERVING NUCLEIC ACID
AU2005245338B2 (en) 2004-04-08 2011-11-17 Biomatrica, Inc. Integration of sample storage and sample management for life science
US20080176209A1 (en) * 2004-04-08 2008-07-24 Biomatrica, Inc. Integration of sample storage and sample management for life science
US20060099567A1 (en) * 2004-04-08 2006-05-11 Biomatrica, Inc. Integration of sample storage and sample management for life science
DE102004023417A1 (de) * 2004-05-12 2005-12-08 Clariant Gmbh Verfahren zur Herstellung von langkettigen quaternären Ammonium-oxalaten und -hydrogenoxalaten
JP4810164B2 (ja) * 2004-09-03 2011-11-09 富士フイルム株式会社 核酸分離精製方法
TWI294460B (en) * 2004-12-23 2008-03-11 Ind Tech Res Inst Method for stabilizing nucleic acids
US20060094023A1 (en) * 2004-11-02 2006-05-04 Industrial Technology Research Institute Method for isolating nucleic acid by using amino surfactants
DE102005015005A1 (de) 2005-04-01 2006-10-05 Qiagen Gmbh Verfahren zur Behandlung einer Biomoleküle enthaltenden Probe
US20060234251A1 (en) * 2005-04-19 2006-10-19 Lumigen, Inc. Methods of enhancing isolation of RNA from biological samples
US20070015165A1 (en) * 2005-07-13 2007-01-18 Sigma-Aldrich Co. Method for the isolation of RNA from biological sources
US20070190526A1 (en) * 2006-02-16 2007-08-16 Nexgen Diagnostics Llc Methods of extracting nucleic acids
WO2007132873A1 (ja) * 2006-05-17 2007-11-22 Yoshiyuki Koyama 核酸、オリゴ核酸、又はその誘導体導入用の凍結乾燥体
DE102007016707A1 (de) * 2007-04-04 2008-10-09 Qiagen Gmbh Verfahren zur Aufreinigung von Biomolekülen
DE102007025276A1 (de) 2007-05-31 2008-12-04 Qiagen Gmbh Aromatische Alkohole zur Behandlung einer biologischen Probe
DE102007025277A1 (de) 2007-05-31 2008-12-04 Qiagen Gmbh Verfahren zur Stabilisierung einer biologischen Probe
DE102007025275A1 (de) 2007-05-31 2008-12-04 Qiagen Gmbh Butendisäure oder deren Derivate zur Behandlung einer biologischen Probe
US20090053704A1 (en) * 2007-08-24 2009-02-26 Natalia Novoradovskaya Stabilization of nucleic acids on solid supports
US7687027B2 (en) * 2008-02-27 2010-03-30 Becton, Dickinson And Company Cleaning compositions, methods and materials for reducing nucleic acid contamination
WO2010108989A1 (en) 2009-03-27 2010-09-30 Université Libre de Bruxelles New marker for diagnosis of active multiple sclerosis
WO2011051402A1 (en) 2009-11-02 2011-05-05 Universite Libre De Bruxelles New biomarkers for determining allergy status
EP2345719A1 (en) * 2010-01-18 2011-07-20 Qiagen GmbH Method for isolating small RNA
US8932809B2 (en) * 2010-01-19 2015-01-13 Opgen, Inc. Methods and kits for isolating nucleic acid from an organism
EP2580348B1 (en) 2010-06-14 2018-04-25 Qiagen GmbH Method for determination of target cells or tissue for extraction of biomolecules from non-formalin-fixed biological samples
EP2407540A1 (en) 2010-07-15 2012-01-18 Qiagen GmbH Method for purifying a target nucleic acid
WO2012018639A2 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
WO2012018638A2 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
CN102146422B (zh) * 2011-01-24 2013-08-07 南京工业大学 一种丁二酸的发酵生产工艺
AU2012242847B2 (en) 2011-04-15 2017-01-19 The Johns Hopkins University Safe sequencing system
EP3150702B1 (en) 2011-06-19 2021-05-19 DNA Genotek, Inc. Devices, solutions and methods for sample collection
DK2742152T3 (en) 2011-08-12 2017-07-31 Qiagen Gmbh PROCEDURE FOR ISOLATING NUCLEIC ACIDS
US11021733B2 (en) 2011-09-26 2021-06-01 Qiagen Gmbh Stabilization and isolation of extracellular nucleic acids
US20140227688A1 (en) 2011-09-26 2014-08-14 Qiagen Gmbh Stabilisation and isolation of extracellular nucleic acids
CN103827303B (zh) 2011-09-26 2021-10-22 普瑞阿那利提克斯有限公司 细胞外核酸的稳定化和分离
CN108103147B (zh) 2012-02-09 2021-06-01 生命技术公司 缀合的聚合物颗粒及其制备方法
EP2647709B1 (en) 2012-04-05 2016-02-24 F. Hoffmann-La Roche AG Amine compounds for the selective preparation of biological samples
US10233508B2 (en) 2012-08-21 2019-03-19 Qiagen Gmbh Virus particle stabilisation and method for isolating viral nucleic acids
US20150225712A1 (en) 2012-08-21 2015-08-13 Qiagen Gmbh Method for isolating nucleic acids from a formaldehyde releaser stabilized sample
WO2014033326A1 (en) * 2012-09-03 2014-03-06 Qiagen Gmbh Method for isolating rna including small rna with high yield
EP2900834B1 (en) 2012-09-25 2016-11-09 Qiagen GmbH Stabilisation of biological samples
ES2701742T3 (es) 2012-10-29 2019-02-25 Univ Johns Hopkins Prueba de Papanicolaou para cánceres de ovario y de endometrio
EP3249054A1 (en) 2012-12-20 2017-11-29 Biomatrica, INC. Formulations and methods for stabilizing pcr reagents
CN110118871A (zh) 2012-12-28 2019-08-13 塞尔雷斯蒂斯有限公司 细胞介导的免疫应答试验
GB201303666D0 (en) 2013-03-01 2013-04-17 Goldsborough Andrew S Sample fixation and stabilisation
WO2014146780A1 (en) 2013-03-18 2014-09-25 Qiagen Gmbh Stabilization and isolation of extracellular nucleic acids
EP2976424B1 (en) 2013-03-18 2018-10-03 Qiagen GmbH Stabilisation of biological samples
GB201305414D0 (en) 2013-03-25 2013-05-08 Arcis Biotechnology Holdings Ltd Method and composition
WO2015131291A1 (en) 2014-03-07 2015-09-11 Dna Genotek Inc. Composition and method for stabilizing nucleic acids in biological samples
EP3119197A1 (en) 2014-03-18 2017-01-25 Qiagen GmbH Stabilization and isolation of extracellular nucleic acids
WO2015191632A1 (en) 2014-06-10 2015-12-17 Biomatrica, Inc. Stabilization of thrombocytes at ambient temperatures
EP3194563B1 (en) 2014-09-17 2023-05-10 Hologic, Inc. Method of partial lysis and assay
CN107667168A (zh) 2015-05-27 2018-02-06 凯杰有限公司 用于破碎组织材料的组合物和方法
CN107922974B (zh) 2015-07-02 2021-11-09 生命技术公司 羧基官能亲水微珠的偶合
WO2017007774A1 (en) 2015-07-06 2017-01-12 Life Technologies Corporation Substrates and methods useful in sequencing
WO2017027653A1 (en) 2015-08-11 2017-02-16 The Johns Hopkins University Assaying ovarian cyst fluid
EP3377645B1 (en) 2015-11-20 2023-10-04 Qiagen GmbH Method of preparing sterilized compositions for stabilization of extracellular nucleic acids
KR102589056B1 (ko) 2015-12-08 2023-10-12 바이오매트리카 인코포레이티드 적혈구 침강 속도의 감소
CN110191960A (zh) * 2016-11-08 2019-08-30 克维拉公司 进行核酸稳定和分离的方法
HUE062204T2 (hu) 2017-01-16 2023-10-28 Spectrum Solutions L L C Nukleinsavkonzerváló oldat és eljárások alkalmazására
CN120400338A (zh) 2017-08-07 2025-08-01 约翰斯霍普金斯大学 用于评估和治疗癌症的方法和材料
CN110012899A (zh) * 2019-04-12 2019-07-16 南京科佰生物科技有限公司 细胞用rna稳定剂及其使用方法
IL295297A (en) 2020-02-14 2022-10-01 Univ Johns Hopkins Methods and materials for assessing nucleic acids
CN116529371A (zh) * 2020-05-29 2023-08-01 洛克菲勒大学 从自收集小体积样品中分离rna的方法和系统
US20230203586A1 (en) * 2020-05-29 2023-06-29 The Rockefeller University Method and system for rna isolation from self-collected and small volume samples
KR102728360B1 (ko) 2021-12-10 2024-11-11 니토 보세키 가부시기가이샤 핵산의 융해 온도 (Tm 값) 상승화제
JP7394301B2 (ja) * 2021-12-10 2023-12-08 日東紡績株式会社 二本鎖核酸の融解温度上昇化剤及びその用途
KR102786563B1 (ko) 2023-04-27 2025-03-26 전남대학교산학협력단 수송배지로서 혈액 내의 핵산 보존용 조성물 및 이를 이용한 핵산의 보존방법

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1289426A (enExample) * 1970-06-22 1972-09-20
US4900677A (en) * 1986-09-26 1990-02-13 E. I. Du Pont De Nemours And Company Process for rapid isolation of high molecular weight DNA
US5010183A (en) * 1989-07-07 1991-04-23 Macfarlane Donald E Process for purifying DNA and RNA using cationic detergents
IT1240870B (it) 1990-02-14 1993-12-17 Talent Procedimento per l'estrazione e la purificazione di dna genomico umano
US5260048A (en) * 1991-05-08 1993-11-09 Streck Laboratories, Inc. Tissue fixative solution and method
US5196182A (en) 1991-05-08 1993-03-23 Streck Laboratories, Inc. Tissue fixative
US5275708A (en) * 1992-03-20 1994-01-04 Thomas Jefferson University Cetyltrimethylammonium bromide gel electrophoresis
CA2107939C (en) * 1993-01-13 2001-01-30 Stephen B. Kong Acidic aqueous cleaning compositions
US5300635A (en) 1993-02-01 1994-04-05 University Of Iowa Research Foundation Quaternary amine surfactants and methods of using same in isolation of nucleic acids
AU6230594A (en) * 1993-02-01 1994-08-29 University Of Iowa Research Foundation, The Quartenary amine surfactants and methods of using same in isolation of rna
US5300645A (en) 1993-04-14 1994-04-05 Eli Lilly And Company Tetrahydro-pyrido-indole
US5641726A (en) * 1993-06-09 1997-06-24 Lonza, Inc. Quaternary ammonium carboxylate and borate compositions and preparation thereof
ZA943999B (en) * 1993-06-09 1995-02-03 Lonza Ag Quaternary ammonium and waterproofing/preservative compositions
JP3256238B2 (ja) 1994-02-11 2002-02-12 クイアジェン・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング 二本鎖／単鎖核酸構造物の分離方法
CA2313654A1 (en) 1997-12-10 1999-06-17 Sierra Diagnostics, Inc. Methods and reagents for preservation of dna in bodily fluids
KR20010033484A (ko) * 1997-12-22 2001-04-25 휴먼 게놈 사이언시즈, 인크. 각질세포 성장 인자-2 제제
US6204375B1 (en) * 1998-07-31 2001-03-20 Ambion, Inc. Methods and reagents for preserving RNA in cell and tissue samples
CA2299119C (en) * 1999-02-23 2013-02-05 Qiagen Gmbh A method of stabilizing and/or isolating nucleic acids
DE10031236A1 (de) * 2000-06-27 2002-01-10 Qiagen Gmbh Verwendung von Carbonsäuren und anderen Additiven in Kombination mit kationischen Verbindungen zur Stabilisierung von Nukleinsäuren in biologischen Materialien

Also Published As

Publication number Publication date
DE10031236A1 (de) 2002-01-10
EP1296932B1 (de) 2008-05-07
PL360705A1 (en) 2004-09-20
EP1820793B1 (de) 2011-09-14
US20080071072A1 (en) 2008-03-20
SK18022002A3 (sk) 2003-07-01
US6861213B2 (en) 2005-03-01
WO2002000600A1 (de) 2002-01-03
AU783922B2 (en) 2005-12-22
MXPA02012261A (es) 2004-09-06
EP1820793A1 (de) 2007-08-22
DK1294676T3 (da) 2008-02-04
CA2410388C (en) 2014-12-09
JP2015212273A (ja) 2015-11-26
AU2007201583A1 (en) 2007-05-03
DE50113941D1 (de) 2008-06-19
DK1820793T3 (da) 2012-01-02
ATE394363T1 (de) 2008-05-15
US20040014703A1 (en) 2004-01-22
AU7568501A (en) 2002-01-08
CZ20024129A3 (cs) 2003-06-18
HUP0301342A2 (hu) 2003-08-28
AU6903101A (en) 2002-01-08
AU2007201583B2 (en) 2010-06-03
EP1296932A1 (de) 2003-04-02
JP2004501622A (ja) 2004-01-22
ATE524431T1 (de) 2011-09-15
CA2412534A1 (en) 2002-12-17
WO2002000599A1 (de) 2002-01-03
AU2001269031B2 (en) 2007-01-11
JP2015027310A (ja) 2015-02-12
JP2004501621A (ja) 2004-01-22
JP5657847B2 (ja) 2015-01-21
EP1294676B1 (de) 2007-10-03
JP2012135317A (ja) 2012-07-19
CA2412534C (en) 2011-08-09
US7682790B2 (en) 2010-03-23
US7270953B2 (en) 2007-09-18
EP1294676A1 (de) 2003-03-26
ES2373784T3 (es) 2012-02-08
JP5795455B2 (ja) 2015-10-14
CA2410388A1 (en) 2002-11-28
BR0112002A (pt) 2003-05-13
US20030165943A1 (en) 2003-09-04
CN1438988A (zh) 2003-08-27
ES2295177T3 (es) 2008-04-16
DE50113086D1 (de) 2007-11-15
ATE374743T1 (de) 2007-10-15

Similar Documents

Publication Publication Date Title
CN1438988A (zh) 由阳离子化合物和质子供体组成的组合物在稳定和/或分离诸如原核生物、真菌、原生动物或藻类这样的微生物中或来自它们中的核酸中的应用
CN1332798A (zh) 从任意复杂起始材料中分离核酸的制剂和方法以及接下来的染色体组基因分析
US20180327802A1 (en) Separatome-based protein expression and purification platform
JP2010154851A (ja) タンパク質抽出薬剤
CN1215177C (zh) 用于鉴定血液中病原菌的基因芯片及其制造方法
CN101052724A (zh) 尿苷5＇－二磷酸－n－乙酰半乳糖胺的制备方法
RU2795623C2 (ru) Способ получения рекомбинантной эндонуклеазы serratia marcescens
EP3650532A1 (en) Methods, apparatus and kits for bacterial cell lysis
US20210380929A1 (en) Methods, apparatus and kits for bacterial cell lysis
CN120424922A (zh) 一种快速简易的质粒提取试剂盒及提取方法
WO2024238499A3 (en) Compositions and methods for purifying viral vectors
WO2024168281A3 (en) Compositions and methods for purifying viral vectors
Wang et al. Engineering for an HPV 9-valent Vaccine using Genomic Constitutive Over-expression and Low Lipopolysaccharide Levels in Escherichia Coli Cells
JPWO2007013264A1 (ja) 新規なエンドリボヌクレアーゼ
JP2009240304A (ja) 溶菌剤
JP2000253896A (ja) Ｌ−スレオ−３−（３，４−ジヒドロキシフェニル）セリン誘導体の製造方法
CN1810969A (zh) 获得具有独立结构/功能蛋白片段的方法

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CX01 Expiry of patent term
CX01 Expiry of patent term

Granted publication date: 20060412