JP2010506591A5 - - Google Patents
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- Publication number
- JP2010506591A5 JP2010506591A5 JP2009533372A JP2009533372A JP2010506591A5 JP 2010506591 A5 JP2010506591 A5 JP 2010506591A5 JP 2009533372 A JP2009533372 A JP 2009533372A JP 2009533372 A JP2009533372 A JP 2009533372A JP 2010506591 A5 JP2010506591 A5 JP 2010506591A5
- Authority
- JP
- Japan
- Prior art keywords
- host cell
- trna
- amino acid
- unnatural amino
- aminoacyl
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 150000001413 amino acids Chemical class 0.000 claims 38
- 238000000034 method Methods 0.000 claims 15
- 102000052866 Amino Acyl-tRNA Synthetases Human genes 0.000 claims 14
- 108700028939 Amino Acyl-tRNA Synthetases Proteins 0.000 claims 14
- 108091033319 polynucleotide Proteins 0.000 claims 11
- 102000040430 polynucleotide Human genes 0.000 claims 11
- 239000002157 polynucleotide Substances 0.000 claims 11
- 102000004169 proteins and genes Human genes 0.000 claims 10
- 108090000623 proteins and genes Proteins 0.000 claims 10
- 108020004705 Codon Proteins 0.000 claims 9
- 102000039446 nucleic acids Human genes 0.000 claims 8
- 108020004707 nucleic acids Proteins 0.000 claims 8
- 150000007523 nucleic acids Chemical class 0.000 claims 8
- 241001112695 Clostridiales Species 0.000 claims 7
- DOCYTUNUHIGJTI-QMMMGPOBSA-N (2r)-2-[(2-nitrophenyl)methylamino]-3-sulfanylpropanoic acid Chemical compound OC(=O)[C@H](CS)NCC1=CC=CC=C1[N+]([O-])=O DOCYTUNUHIGJTI-QMMMGPOBSA-N 0.000 claims 6
- 241000288906 Primates Species 0.000 claims 5
- 241000283984 Rodentia Species 0.000 claims 5
- 241000588724 Escherichia coli Species 0.000 claims 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 4
- VKBLQCDGTHFOLS-NSHDSACASA-N (2s)-2-(4-benzoylanilino)propanoic acid Chemical compound C1=CC(N[C@@H](C)C(O)=O)=CC=C1C(=O)C1=CC=CC=C1 VKBLQCDGTHFOLS-NSHDSACASA-N 0.000 claims 3
- JSXMFBNJRFXRCX-NSHDSACASA-N (2s)-2-amino-3-(4-prop-2-ynoxyphenyl)propanoic acid Chemical compound OC(=O)[C@@H](N)CC1=CC=C(OCC#C)C=C1 JSXMFBNJRFXRCX-NSHDSACASA-N 0.000 claims 3
- NEMHIKRLROONTL-QMMMGPOBSA-N (2s)-2-azaniumyl-3-(4-azidophenyl)propanoate Chemical compound OC(=O)[C@@H](N)CC1=CC=C(N=[N+]=[N-])C=C1 NEMHIKRLROONTL-QMMMGPOBSA-N 0.000 claims 3
- ZXSBHXZKWRIEIA-JTQLQIEISA-N (2s)-3-(4-acetylphenyl)-2-azaniumylpropanoate Chemical compound CC(=O)C1=CC=C(C[C@H](N)C(O)=O)C=C1 ZXSBHXZKWRIEIA-JTQLQIEISA-N 0.000 claims 3
- QHGDJQUCSGUYMF-QMMMGPOBSA-N (2s)-3-hydroxy-2-[(2-nitrophenyl)methylamino]propanoic acid Chemical compound OC[C@@H](C(O)=O)NCC1=CC=CC=C1[N+]([O-])=O QHGDJQUCSGUYMF-QMMMGPOBSA-N 0.000 claims 3
- AKVBCGQVQXPRLD-UHFFFAOYSA-N 2-aminooctanoic acid Chemical compound CCCCCCC(N)C(O)=O AKVBCGQVQXPRLD-UHFFFAOYSA-N 0.000 claims 3
- PZNQZSRPDOEBMS-QMMMGPOBSA-N 4-iodo-L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(I)C=C1 PZNQZSRPDOEBMS-QMMMGPOBSA-N 0.000 claims 3
- GEYBMYRBIABFTA-VIFPVBQESA-N O-methyl-L-tyrosine Chemical compound COC1=CC=C(C[C@H](N)C(O)=O)C=C1 GEYBMYRBIABFTA-VIFPVBQESA-N 0.000 claims 3
- 241001442654 Percnon planissimum Species 0.000 claims 3
- 241001425800 Pipa Species 0.000 claims 3
- 239000002773 nucleotide Substances 0.000 claims 3
- 125000003729 nucleotide group Chemical group 0.000 claims 3
- 238000002741 site-directed mutagenesis Methods 0.000 claims 3
- 241000282575 Gorilla Species 0.000 claims 2
- 241000282620 Hylobates sp. Species 0.000 claims 2
- 241000282553 Macaca Species 0.000 claims 2
- 241000282576 Pan paniscus Species 0.000 claims 2
- 241000282577 Pan troglodytes Species 0.000 claims 2
- 241000282405 Pongo abelii Species 0.000 claims 2
- 241000288961 Saguinus imperator Species 0.000 claims 2
- 230000000689 aminoacylating effect Effects 0.000 claims 2
- 238000012258 culturing Methods 0.000 claims 2
- 230000001678 irradiating effect Effects 0.000 claims 2
- RCRODHONKLSMIF-UHFFFAOYSA-N isosuberenol Natural products O1C(=O)C=CC2=C1C=C(OC)C(CC(O)C(C)=C)=C2 RCRODHONKLSMIF-UHFFFAOYSA-N 0.000 claims 2
- 241001515942 marmosets Species 0.000 claims 2
- 241000193385 Geobacillus stearothermophilus Species 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 230000004952 protein activity Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US85300806P | 2006-10-18 | 2006-10-18 | |
| US92345807P | 2007-04-12 | 2007-04-12 | |
| PCT/US2007/022232 WO2008073184A2 (en) | 2006-10-18 | 2007-10-17 | Genetic incorporation of unnatural amino acids into proteins in mammalian cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2010506591A JP2010506591A (ja) | 2010-03-04 |
| JP2010506591A5 true JP2010506591A5 (enExample) | 2010-12-09 |
Family
ID=39512252
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009533372A Pending JP2010506591A (ja) | 2006-10-18 | 2007-10-17 | 哺乳動物細胞中の蛋白質への非天然アミノ酸の遺伝的組込み |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US20080166766A1 (enExample) |
| EP (1) | EP2069481A4 (enExample) |
| JP (1) | JP2010506591A (enExample) |
| KR (1) | KR20090094812A (enExample) |
| CN (1) | CN101535338A (enExample) |
| AU (1) | AU2007333010A1 (enExample) |
| BR (1) | BRPI0718389A2 (enExample) |
| CA (1) | CA2665678A1 (enExample) |
| IL (1) | IL197917A0 (enExample) |
| MX (1) | MX2009004043A (enExample) |
| RU (1) | RU2009112104A (enExample) |
| WO (1) | WO2008073184A2 (enExample) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1490483B1 (en) * | 2001-04-19 | 2015-06-03 | The Scripps Research Institute | In vivo incorporation of unnatural amino acids |
| KR20050073559A (ko) * | 2002-10-16 | 2005-07-14 | 더 스크립스 리서치 인스티튜트 | 당단백질 합성 |
| CN103289960B (zh) | 2003-04-17 | 2017-04-26 | 斯克利普斯研究院 | 扩展真核生物遗传密码 |
| DE602004011789T2 (de) * | 2003-07-07 | 2009-02-12 | The Scripps Research Institute, La Jolla | Zusammensetzungen der orthogonalen Lysyl-tRNA und Aminoacyl-tRNA Synthetase Paaren und ihre Verwendungen |
| WO2010114615A2 (en) * | 2009-04-03 | 2010-10-07 | The Scripps Research Institute | A facile system for encoding unnatural amino acids in mammalian cells |
| EP2647721B1 (en) | 2010-12-03 | 2019-02-27 | The University of Tokyo | Peptide with safer secondary structure, peptide library, and production methods for same |
| DE102010056289A1 (de) | 2010-12-24 | 2012-06-28 | Geneart Ag | Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken |
| BR112015006368B1 (pt) * | 2012-09-24 | 2022-02-08 | Medimmune Limited | Processo para produção de linha celular eucariótica estável |
| BR112015022641B1 (pt) * | 2013-03-15 | 2023-02-28 | Medimmune Limited | Construção de dna e processo de preparação de uma proteína-alvo |
| CN105420203B (zh) * | 2014-09-03 | 2018-11-16 | 中国科学院生物物理研究所 | 酪氨酸类似物翻译系统和基因编码的蛋白质光致电子转移荧光传感器蛋白家族 |
| CN105400816A (zh) * | 2015-11-24 | 2016-03-16 | 深圳市第二人民医院 | 用于膀胱癌检测的逻辑与门基因线路的构建方法 |
| GB201600512D0 (en) | 2016-01-12 | 2016-02-24 | Univ York | Recombinant protein production |
| CN112725282A (zh) * | 2016-01-27 | 2021-04-30 | 北京大学 | 携带正交tRNA/氨酰tRNA合成酶的稳定细胞系的构建 |
| AU2018221227A1 (en) | 2017-02-17 | 2019-08-15 | Rubius Therapeutics, Inc. | Functionalized erythroid cells |
| CN117286182A (zh) * | 2017-06-02 | 2023-12-26 | Ambrx公司 | 促进含非天然氨基酸的蛋白质产生的方法和组合物 |
| EP3634988A4 (en) | 2017-06-06 | 2021-02-24 | Kindred Biosciences, Inc. | ERYTHROPOIETIN AND SIMILAR COMPOUNDS FOR VETERINARY USE |
| US20210062176A1 (en) | 2018-04-19 | 2021-03-04 | Kindred Biosciences, Inc. | Variant Asparaginase Polypeptides for Medical Use |
| US20210356377A1 (en) * | 2018-09-07 | 2021-11-18 | Medimmune, Llc | Methods Of Cell Selection |
| US20220325269A1 (en) * | 2019-08-08 | 2022-10-13 | Brickbio, Inc. | Aminoacyl-trna synthetases and cell lines for site-specific incorporation of unnatural amino acids |
| JP2022552137A (ja) * | 2019-10-15 | 2022-12-15 | トラスティーズ オブ ボストン カレッジ | 非天然アミノ酸組み込み増強のためのキメラ耐熱性アミノアシルｔＲＮＡシンテターゼ |
| WO2021117848A1 (ja) * | 2019-12-12 | 2021-06-17 | 中外製薬株式会社 | 非天然アミノ酸を含むペプチドの製造方法 |
| CN113388654A (zh) * | 2021-06-04 | 2021-09-14 | 清华大学 | 一种基于线形模板无细胞体系非天然氨基酸嵌入方法 |
| CN114134141B (zh) * | 2021-11-03 | 2024-01-30 | 杭州嵌化合生医药科技有限公司 | 一种引入非天然氨基酸的嵌合体苯丙氨酸翻译系统及其构建方法 |
| CN119662697A (zh) * | 2024-12-20 | 2025-03-21 | 天津大学 | 缺失基因的酿酒酵母及在高效利用非天然氨基酸修饰蛋白质中的应用 |
Family Cites Families (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1490483B1 (en) | 2001-04-19 | 2015-06-03 | The Scripps Research Institute | In vivo incorporation of unnatural amino acids |
| KR20050073559A (ko) | 2002-10-16 | 2005-07-14 | 더 스크립스 리서치 인스티튜트 | 당단백질 합성 |
| DK1578949T3 (da) | 2002-10-16 | 2009-07-27 | Scripps Research Inst | Stedspecifik optagelse af keto-aminnosyrer hos proteiner |
| CA2503749A1 (en) * | 2002-10-31 | 2004-05-13 | Riken | Method of expressing protein having unnatural amino acid integrated thereinto |
| EP1583816A4 (en) | 2002-12-22 | 2007-06-13 | Scripps Research Inst | PROTEIN ARRAYS |
| EA200700469A1 (ru) * | 2003-02-14 | 2008-10-30 | Байоджен Айдек Ма Инк. | Экспрессионная кассета и вектор для временной или постоянной экспрессии экзогенных молекул |
| CN103289960B (zh) | 2003-04-17 | 2017-04-26 | 斯克利普斯研究院 | 扩展真核生物遗传密码 |
| WO2005003294A2 (en) * | 2003-06-18 | 2005-01-13 | The Scripps Research Institute | Unnatural reactive amino acid genetic code additions |
| WO2005007870A2 (en) | 2003-07-07 | 2005-01-27 | The Scripps Research Institute | COMPOSITIONS OF ORTHOGONAL LEUCYL-tRNA AND AMINOACYL-tRNA SYNTHETASE PAIRS AND USES THEREOF |
| DE602004011789T2 (de) | 2003-07-07 | 2009-02-12 | The Scripps Research Institute, La Jolla | Zusammensetzungen der orthogonalen Lysyl-tRNA und Aminoacyl-tRNA Synthetase Paaren und ihre Verwendungen |
| WO2005007624A2 (en) | 2003-07-07 | 2005-01-27 | The Scripps Research Institute | Compositions of orthogonal glutamyl-trna and aminoacyl trna synthetase pairs and uses thereof |
| WO2005038002A2 (en) | 2003-10-14 | 2005-04-28 | The Scripps Research Institute | Site-specific incorporation of redox active amino acids into proteins |
| BRPI0417801A (pt) | 2003-12-18 | 2007-04-10 | Scripps Research Inst | incorporação seletiva de 5-hidroxitriptofano em proteìnas de células mamìferas |
| BRPI0507159A (pt) | 2004-02-02 | 2007-06-26 | Ambrx Inc | polipeptìdeos em feixe de quatro hélices humanos modificados e seus usos |
| AU2005248392A1 (en) | 2004-05-25 | 2005-12-08 | Irm, Llc | Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for crystal structure determination |
| EP1797177A4 (en) * | 2004-09-21 | 2008-07-09 | Scripps Research Inst | ADDITION OF PHOTOREGULATED AMINO ACIDS TO THE GENETIC CODE |
| EP1794323B1 (en) | 2004-09-21 | 2014-08-06 | The Scripps Research Institute | In vivo incorporation of alkynyl amino acids into proteins in eubacteria |
| JP2008513806A (ja) | 2004-09-22 | 2008-05-01 | ザ スクリップス リサーチ インスティテュート | Ｎｍｒ試験のための蛋白質の部位特異的標識 |
| EP1809758A4 (en) | 2004-10-20 | 2009-04-01 | Scripps Research Inst | LOCAL IN VIVO INSTALLATION OF N-ACETYLGALACTOSAMINE AMINO ACIDS IN EU BACTERIA |
| AU2005330514B2 (en) | 2004-10-27 | 2011-05-12 | The Scripps Research Institute | Orthogonal translation components for the in vivo incorporation of unnatural amino acids |
| CA2624290A1 (en) | 2005-10-12 | 2007-04-26 | Scripps Research Institute | Selective posttranslational modification of phage-displayed polypeptides |
| CA2638763A1 (en) | 2006-03-09 | 2007-09-13 | The Scripps Research Institute | System for the expression of orthogonal translation components in eubacterial host cells |
| JP5249194B2 (ja) | 2006-03-16 | 2013-07-31 | ザ スクリプス リサーチ インスティチュート | 非天然アミノ酸フェニルセレノシステインを含有するタンパク質の遺伝的にプログラムされた発現 |
-
2007
- 2007-10-17 US US11/975,351 patent/US20080166766A1/en not_active Abandoned
- 2007-10-17 WO PCT/US2007/022232 patent/WO2008073184A2/en not_active Ceased
- 2007-10-17 AU AU2007333010A patent/AU2007333010A1/en not_active Abandoned
- 2007-10-17 CN CNA2007800390157A patent/CN101535338A/zh active Pending
- 2007-10-17 RU RU2009112104/10A patent/RU2009112104A/ru not_active Application Discontinuation
- 2007-10-17 MX MX2009004043A patent/MX2009004043A/es not_active Application Discontinuation
- 2007-10-17 CA CA002665678A patent/CA2665678A1/en not_active Abandoned
- 2007-10-17 JP JP2009533372A patent/JP2010506591A/ja active Pending
- 2007-10-17 EP EP07870795A patent/EP2069481A4/en not_active Withdrawn
- 2007-10-17 KR KR1020097009346A patent/KR20090094812A/ko not_active Ceased
- 2007-10-17 US US12/311,545 patent/US9624485B2/en not_active Expired - Fee Related
- 2007-10-17 BR BRPI0718389-5A patent/BRPI0718389A2/pt not_active IP Right Cessation
-
2009
- 2009-04-05 IL IL197917A patent/IL197917A0/en unknown
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