MX2009004043A - Incorporacion genetica de aminoacidos no naturales a proteinas en celulas mamiferas. - Google Patents

Incorporacion genetica de aminoacidos no naturales a proteinas en celulas mamiferas.

Info

Publication number
MX2009004043A
MX2009004043A MX2009004043A MX2009004043A MX2009004043A MX 2009004043 A MX2009004043 A MX 2009004043A MX 2009004043 A MX2009004043 A MX 2009004043A MX 2009004043 A MX2009004043 A MX 2009004043A MX 2009004043 A MX2009004043 A MX 2009004043A
Authority
MX
Mexico
Prior art keywords
unnatural amino
proteins
amino acids
host cells
mammalian cells
Prior art date
Application number
MX2009004043A
Other languages
English (en)
Inventor
Peter G Schultz
Wenshe Liu
Original Assignee
Scripps Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Inst filed Critical Scripps Research Inst
Publication of MX2009004043A publication Critical patent/MX2009004043A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La invención es concerniente con pares ortogonales de tARN t aminoacil-tARN sintetasas que pueden incorporar aminoácidos no naturales a proteínas en células huésped mamíferas, por ejemplo, células huésped de primate y células huésped de roedor. La invención proporciona, por ejemplo pero no limitado a, sistemas de traducción que incluyen células huésped (por ejemplo, células de primate o células de roedor), aminoacil-tARN sintetasas ortogonales derivadas de sintetasas eubacterianas, tARN ortogonales y el aminoácido no natural. La invención también es concerniente con métodos para producir proteínas de interés que comprenden por lo menos un aminoácido no natural en sistemas de célula huésped mamíferos.
MX2009004043A 2006-10-18 2007-10-17 Incorporacion genetica de aminoacidos no naturales a proteinas en celulas mamiferas. MX2009004043A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US85300806P 2006-10-18 2006-10-18
US92345807P 2007-04-12 2007-04-12
PCT/US2007/022232 WO2008073184A2 (en) 2006-10-18 2007-10-17 Genetic incorporation of unnatural amino acids into proteins in mammalian cells

Publications (1)

Publication Number Publication Date
MX2009004043A true MX2009004043A (es) 2009-04-27

Family

ID=39512252

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2009004043A MX2009004043A (es) 2006-10-18 2007-10-17 Incorporacion genetica de aminoacidos no naturales a proteinas en celulas mamiferas.

Country Status (12)

Country Link
US (2) US9624485B2 (es)
EP (1) EP2069481A4 (es)
JP (1) JP2010506591A (es)
KR (1) KR20090094812A (es)
CN (1) CN101535338A (es)
AU (1) AU2007333010A1 (es)
BR (1) BRPI0718389A2 (es)
CA (1) CA2665678A1 (es)
IL (1) IL197917A0 (es)
MX (1) MX2009004043A (es)
RU (1) RU2009112104A (es)
WO (1) WO2008073184A2 (es)

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CA2444098C (en) * 2001-04-19 2016-06-21 The Scripps Research Institute Methods and composition for the production of orthogonal trna-aminoacyltrna synthetase pairs
WO2004035605A2 (en) * 2002-10-16 2004-04-29 The Scripps Research Institute Glycoprotein synthesis
CN103215228A (zh) 2003-04-17 2013-07-24 斯克利普斯研究院 扩展真核生物遗传密码
DE602004011789T2 (de) 2003-07-07 2009-02-12 The Scripps Research Institute, La Jolla Zusammensetzungen der orthogonalen Lysyl-tRNA und Aminoacyl-tRNA Synthetase Paaren und ihre Verwendungen
WO2010114615A2 (en) * 2009-04-03 2010-10-07 The Scripps Research Institute A facile system for encoding unnatural amino acids in mammalian cells
CN103328648B (zh) * 2010-12-03 2015-11-25 国立大学法人东京大学 具有稳定的二级结构的肽和肽库以及它们的制造方法
DE102010056289A1 (de) 2010-12-24 2012-06-28 Geneart Ag Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken
WO2014044872A1 (en) * 2012-09-24 2014-03-27 Allozyne, Inc Cell lines
JP6550339B2 (ja) * 2013-03-15 2019-07-24 メディミューン リミテッド 新規の核酸分子
CN105420203B (zh) * 2014-09-03 2018-11-16 中国科学院生物物理研究所 酪氨酸类似物翻译系统和基因编码的蛋白质光致电子转移荧光传感器蛋白家族
CN105400816A (zh) * 2015-11-24 2016-03-16 深圳市第二人民医院 用于膀胱癌检测的逻辑与门基因线路的构建方法
GB201600512D0 (en) 2016-01-12 2016-02-24 Univ York Recombinant protein production
CN107012121B (zh) * 2016-01-27 2021-01-26 北京大学 携带正交tRNA/氨酰tRNA合成酶的稳定细胞系的构建
US11020435B2 (en) 2017-02-17 2021-06-01 Rubius Therapeutics, Inc. Functionalized erythroid cells
WO2018223108A1 (en) * 2017-06-02 2018-12-06 Ambrx, Inc. Methods and compositions for promoting non-natural amino acid-containing protein production
EP3634988A4 (en) 2017-06-06 2021-02-24 Kindred Biosciences, Inc. ERYTHROPOIETIN AND ANALOGUE FOR USE IN VETERINARY MEDICINE
JP2021520828A (ja) 2018-04-19 2021-08-26 キンドレッド バイオサイエンシズ インコーポレイテッド 医療用の変異体アスパラギナーゼポリペプチド
JP2021536263A (ja) * 2018-09-07 2021-12-27 メディミューン，エルエルシー 細胞選択の方法
CA3150276A1 (en) * 2019-08-08 2021-02-11 Brickbio, Inc. Aminoacyl-trna synthetases and cell lines for site-specific incorporation of unnatural amino acids
EP4074829A4 (en) * 2019-12-12 2024-01-10 Chugai Pharmaceutical Co Ltd METHOD FOR PRODUCING PEPTIDE CONTAINING NON-NATURAL AMINO ACID
CN113388654A (zh) * 2021-06-04 2021-09-14 清华大学 一种基于线形模板无细胞体系非天然氨基酸嵌入方法
CN114134141B (zh) * 2021-11-03 2024-01-30 杭州嵌化合生医药科技有限公司 一种引入非天然氨基酸的嵌合体苯丙氨酸翻译系统及其构建方法

Family Cites Families (23)

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Publication number Priority date Publication date Assignee Title
CA2444098C (en) 2001-04-19 2016-06-21 The Scripps Research Institute Methods and composition for the production of orthogonal trna-aminoacyltrna synthetase pairs
PT1578949E (pt) 2002-10-16 2009-07-14 Scripps Research Inst Incorporação específica do local de cetoaminoácidos em proteínas
WO2004035605A2 (en) 2002-10-16 2004-04-29 The Scripps Research Institute Glycoprotein synthesis
US7566555B2 (en) * 2002-10-31 2009-07-28 Riken Method of expressing proteins comprising non-naturally-occurring amino acids
MXPA05006836A (es) 2002-12-22 2005-08-16 Scripps Research Inst Colecciones ordenadas de proteinas.
ATE553185T1 (de) * 2003-02-14 2012-04-15 Biogen Idec Inc Expressionskassette und vektor zur transienten oder stabilen expression exogener moleküle
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MXPA06013586A (es) 2004-05-25 2007-11-09 Scripss Res Inst Incorporacion especifica del sitio de aminoacidos no naturales que contienen atomos pesados en proteinas para la determinacion de la estructura cristalina.
JP2008513039A (ja) 2004-09-21 2008-05-01 ザ スクリップス リサーチ インスティテュート 真正細菌の蛋白質へのアルキニルアミノ酸のインビボ組込み
EP1797177A4 (en) * 2004-09-21 2008-07-09 Scripps Research Inst ADDITION OF PHOTOREGULATED AMINO ACIDS TO THE GENETIC CODE
JP2008513806A (ja) 2004-09-22 2008-05-01 ザ スクリップス リサーチ インスティテュート Ｎｍｒ試験のための蛋白質の部位特異的標識
WO2006045116A2 (en) 2004-10-20 2006-04-27 The Scripps Research Institute In vivo site-specific incorporation of n-acetyl-galactosamine amino acids in eubacteria
JP5135504B2 (ja) 2004-10-27 2013-02-06 ザ スクリプス リサーチ インスティチュート 非天然アミノ酸のインビボ組込み用直交翻訳成分
BRPI0617191A2 (pt) 2005-10-12 2011-07-19 Scripps Research Inst modificação pós-traducional de polipeptìdeos expressos em fagos
WO2007103490A2 (en) 2006-03-09 2007-09-13 The Scripps Research Institute System for the expression of orthogonal translation components in eubacterial host cells
CN101405401B (zh) 2006-03-16 2013-03-27 斯克利普斯研究院 含非天然氨基酸苯基硒代半胱氨酸的蛋白质的遗传编程表达

Also Published As

Publication number Publication date
BRPI0718389A2 (pt) 2012-04-10
KR20090094812A (ko) 2009-09-08
WO2008073184A2 (en) 2008-06-19
RU2009112104A (ru) 2010-11-27
WO2008073184A3 (en) 2008-11-27
CA2665678A1 (en) 2008-06-19
CN101535338A (zh) 2009-09-16
EP2069481A4 (en) 2010-01-06
US20080166766A1 (en) 2008-07-10
US9624485B2 (en) 2017-04-18
US20100021963A1 (en) 2010-01-28
JP2010506591A (ja) 2010-03-04
AU2007333010A1 (en) 2008-06-19
EP2069481A2 (en) 2009-06-17
IL197917A0 (en) 2011-08-01

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