MX2009002987A - Expresion geneticamente programada de proteinas selectivamente sulfatadas en eubacterias. - Google Patents

Expresion geneticamente programada de proteinas selectivamente sulfatadas en eubacterias.

Info

Publication number
MX2009002987A
MX2009002987A MX2009002987A MX2009002987A MX2009002987A MX 2009002987 A MX2009002987 A MX 2009002987A MX 2009002987 A MX2009002987 A MX 2009002987A MX 2009002987 A MX2009002987 A MX 2009002987A MX 2009002987 A MX2009002987 A MX 2009002987A
Authority
MX
Mexico
Prior art keywords
eubacteria
novel
genetically programmed
programmed expression
sulfated proteins
Prior art date
Application number
MX2009002987A
Other languages
English (en)
Inventor
Peter G Schultz
Chang C Liu
Original Assignee
Scripps Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Inst filed Critical Scripps Research Inst
Publication of MX2009002987A publication Critical patent/MX2009002987A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Abstract

La invención se refiere a pares ortogonales de ARNts y aminoacil-ARNt sintetasas que pueden incorporar la sulfotirosina de aminoácido no natural en proteínas producidas en células huésped eubacterianas tales como E.coli. La invención proporciona, por ejemplo, pero sin limitación, nuevas aminoacil-ARNt sintetasas ortogonales, polinucleótidos que codifican para las nuevas moléculas de sintetasa, métodos para identificar y producir las nuevas sintetasas, métodos para producir proteínas que contienen la sulfotirosina de aminoácido no natural y sistemas de traducción.
MX2009002987A 2006-09-21 2007-09-20 Expresion geneticamente programada de proteinas selectivamente sulfatadas en eubacterias. MX2009002987A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US84651906P 2006-09-21 2006-09-21
US85521006P 2006-10-28 2006-10-28
PCT/US2007/020459 WO2008036392A2 (en) 2006-09-21 2007-09-20 Genetically programmed expression of selectively sulfated proteins in eubacteria

Publications (1)

Publication Number Publication Date
MX2009002987A true MX2009002987A (es) 2009-04-28

Family

ID=39201110

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2009002987A MX2009002987A (es) 2006-09-21 2007-09-20 Expresion geneticamente programada de proteinas selectivamente sulfatadas en eubacterias.

Country Status (15)

Country Link
US (3) US8114652B2 (es)
EP (1) EP2052083B1 (es)
JP (1) JP5385143B2 (es)
KR (1) KR20090074765A (es)
CN (1) CN101516901B (es)
AU (1) AU2007297610A1 (es)
BR (1) BRPI0716963A2 (es)
CA (1) CA2663052A1 (es)
ES (1) ES2456331T3 (es)
IL (1) IL197531A0 (es)
MX (1) MX2009002987A (es)
PL (1) PL2052083T3 (es)
PT (1) PT2052083E (es)
RU (1) RU2009110152A (es)
WO (1) WO2008036392A2 (es)

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US6927042B2 (en) * 2002-10-16 2005-08-09 The Scripps Research Institute Glycoprotein synthesis
WO2004094593A2 (en) 2003-04-17 2004-11-04 The Scripps Research Institute Expanding the eukaryotic genetic code
DE602004011789T2 (de) 2003-07-07 2009-02-12 The Scripps Research Institute, La Jolla Zusammensetzungen der orthogonalen Lysyl-tRNA und Aminoacyl-tRNA Synthetase Paaren und ihre Verwendungen
AU2008325239A1 (en) * 2007-11-02 2009-05-14 The Scripps Research Institute Directed evolution using proteins comprising unnatural amino acids
US8658394B2 (en) * 2011-02-02 2014-02-25 Waterstone Pharmaceuticals Methods, cells and systems for incorporating non-canonical amino acids into proteins
CN102888387B (zh) * 2011-07-21 2015-03-18 中国科学院生物物理研究所 3-氯代酪氨酸翻译系统及其应用
CN103571804B (zh) * 2012-08-10 2015-08-12 中国科学院生物物理研究所 3-吡唑基酪氨酸翻译系统及其应用
US10501734B2 (en) 2014-02-06 2019-12-10 Yale University Compositions and methods of use thereof for making polypeptides with many instances of nonstandard amino acids
WO2016073079A2 (en) 2014-09-26 2016-05-12 Yale University Compositions and methods for biocontainment of microorganisms
CN110117580B (zh) * 2018-02-05 2021-12-14 中国科学院生物物理研究所 硒代酪氨酸翻译系统及其应用
KR20210034037A (ko) * 2018-07-18 2021-03-29 리젠엑스바이오 인크. 완전-인간 글리코실화된 인간 알파-l-이두로니다아제 (idua)를 사용한 점액다당류증 i의 치료
WO2020180971A1 (en) * 2019-03-04 2020-09-10 The Texas A&M University System Methods of making and utilizing amber-obligated phage display libraries
US11149280B2 (en) 2019-10-29 2021-10-19 Yale University Engineering organisms resistant to viruses and horizontally transferred genetic elements
CN111304234A (zh) * 2020-02-27 2020-06-19 江南大学 一种适用于枯草芽孢杆菌的非天然氨基酸利用工具
CN113789335B (zh) * 2021-11-16 2022-03-15 江苏省中国科学院植物研究所 一种水蛭酪氨酸磺酸转移酶基因及其应用

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US6204016B1 (en) * 1998-01-29 2001-03-20 The Board Of Regents Of The University Of Oklahoma Tyrosylprotein sulfotransferases and methods of use thereof
DK1456360T3 (en) * 2001-04-19 2015-08-31 Scripps Research Inst Methods and Composition for Preparation of Orthogonal TRNA-Aminoacyl-TRNA Synthetase Pairs
DE60327369D1 (de) 2002-10-16 2009-06-04 Scripps Research Inst Stellenspezifischer einbau von ketoaminosäuren in proteine
US6927042B2 (en) 2002-10-16 2005-08-09 The Scripps Research Institute Glycoprotein synthesis
CA2508939A1 (en) 2002-12-22 2004-07-15 The Scripps Research Institute Protein arrays
WO2004094593A2 (en) 2003-04-17 2004-11-04 The Scripps Research Institute Expanding the eukaryotic genetic code
CN102618605B (zh) 2003-06-18 2015-09-02 斯克利普斯研究院 非天然活性氨基酸遗传密码增加
DE602004011789T2 (de) 2003-07-07 2009-02-12 The Scripps Research Institute, La Jolla Zusammensetzungen der orthogonalen Lysyl-tRNA und Aminoacyl-tRNA Synthetase Paaren und ihre Verwendungen
WO2005007870A2 (en) 2003-07-07 2005-01-27 The Scripps Research Institute COMPOSITIONS OF ORTHOGONAL LEUCYL-tRNA AND AMINOACYL-tRNA SYNTHETASE PAIRS AND USES THEREOF
EP1658366A4 (en) 2003-07-07 2006-08-09 Scripps Research Inst COMPOSITIONS OF ORTHOGONAL GLUTAMYL-TRNA AND AMINOACYL-TRNA-SYNTHETASEPAARES AND THEIR USES
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Also Published As

Publication number Publication date
US8114627B2 (en) 2012-02-14
EP2052083A2 (en) 2009-04-29
KR20090074765A (ko) 2009-07-07
AU2007297610A1 (en) 2008-03-27
JP5385143B2 (ja) 2014-01-08
BRPI0716963A2 (pt) 2013-11-05
CN101516901A (zh) 2009-08-26
WO2008036392A3 (en) 2008-12-31
PL2052083T3 (pl) 2014-06-30
JP2010504096A (ja) 2010-02-12
PT2052083E (pt) 2014-04-10
WO2008036392A2 (en) 2008-03-27
EP2052083B1 (en) 2014-01-08
EP2052083A4 (en) 2010-03-24
US8114652B2 (en) 2012-02-14
RU2009110152A (ru) 2010-10-27
ES2456331T3 (es) 2014-04-22
WO2008036392A9 (en) 2008-05-22
US20080113407A1 (en) 2008-05-15
IL197531A0 (en) 2009-12-24
CN101516901B (zh) 2014-01-08
US8361770B2 (en) 2013-01-29
CA2663052A1 (en) 2008-03-27
US20120184018A1 (en) 2012-07-19
US20090286279A1 (en) 2009-11-19

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