ATE542894T1 - Ausweitung des eukaryotischen genetischen codes - Google Patents

Ausweitung des eukaryotischen genetischen codes

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Publication number
ATE542894T1
ATE542894T1 AT04759917T AT04759917T ATE542894T1 AT E542894 T1 ATE542894 T1 AT E542894T1 AT 04759917 T AT04759917 T AT 04759917T AT 04759917 T AT04759917 T AT 04759917T AT E542894 T1 ATE542894 T1 AT E542894T1
Authority
AT
Austria
Prior art keywords
amino acids
expansion
genetic code
eukaryotic genetic
orthogonal
Prior art date
Application number
AT04759917T
Other languages
English (en)
Inventor
Jason Chin
Ashton Cropp
Christopher Anderson
Peter Schultz
Original Assignee
Scripps Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Scripps Research Inst filed Critical Scripps Research Inst
Application granted granted Critical
Publication of ATE542894T1 publication Critical patent/ATE542894T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/1703—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • A61K38/1709—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y601/00—Ligases forming carbon-oxygen bonds (6.1)
    • C12Y601/01—Ligases forming aminoacyl-tRNA and related compounds (6.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y601/00—Ligases forming carbon-oxygen bonds (6.1)
    • C12Y601/01—Ligases forming aminoacyl-tRNA and related compounds (6.1.1)
    • C12Y601/01001—Tyrosine-tRNA ligase (6.1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y601/00—Ligases forming carbon-oxygen bonds (6.1)
    • C12Y601/01—Ligases forming aminoacyl-tRNA and related compounds (6.1.1)
    • C12Y601/0102—Phenylalanine-tRNA ligase (6.1.1.20)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Mycology (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Epidemiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
AT04759917T 2003-04-17 2004-04-16 Ausweitung des eukaryotischen genetischen codes ATE542894T1 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US46386903P 2003-04-17 2003-04-17
US47993103P 2003-06-18 2003-06-18
US49301403P 2003-08-05 2003-08-05
US49654803P 2003-08-19 2003-08-19
PCT/US2004/011786 WO2004094593A2 (en) 2003-04-17 2004-04-16 Expanding the eukaryotic genetic code

Publications (1)

Publication Number Publication Date
ATE542894T1 true ATE542894T1 (de) 2012-02-15

Family

ID=33314444

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04759917T ATE542894T1 (de) 2003-04-17 2004-04-16 Ausweitung des eukaryotischen genetischen codes

Country Status (12)

Country Link
US (10) US7618775B2 (de)
EP (1) EP1613735B1 (de)
JP (2) JP5642916B2 (de)
KR (1) KR101171397B1 (de)
CN (4) CN103289960B (de)
AT (1) ATE542894T1 (de)
AU (2) AU2004233083B2 (de)
CA (1) CA2520750A1 (de)
HK (1) HK1087432A1 (de)
IL (1) IL171128A (de)
MX (1) MXPA05011127A (de)
WO (1) WO2004094593A2 (de)

Families Citing this family (95)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2444098C (en) * 2001-04-19 2016-06-21 The Scripps Research Institute Methods and composition for the production of orthogonal trna-aminoacyltrna synthetase pairs
WO2004035605A2 (en) 2002-10-16 2004-04-29 The Scripps Research Institute Glycoprotein synthesis
JP5642916B2 (ja) 2003-04-17 2014-12-17 ザ スクリプス リサーチ インスティテュート 真核遺伝コードの拡張
EP2392923B1 (de) 2003-06-18 2014-05-21 The Scripps Research Institute Methode zur Herstellung von Proteinen mit unnatürlichen Aminosäuren
ES2301099T3 (es) * 2003-07-07 2008-06-16 The Scripps Research Institute Composiciones de parejas ortogonales lisil-arnt y aminoacil-arnt sintetasa y usos de las mismas.
EP1658366A4 (de) 2003-07-07 2006-08-09 Scripps Research Inst Zusammensetzungen von orthogonalen glutamyl-trna- und aminoacyl-trna-synthetasepaaren und deren verwendungen
WO2005007870A2 (en) * 2003-07-07 2005-01-27 The Scripps Research Institute COMPOSITIONS OF ORTHOGONAL LEUCYL-tRNA AND AMINOACYL-tRNA SYNTHETASE PAIRS AND USES THEREOF
PT1687401E (pt) 2003-10-14 2013-02-26 Scripps Research Inst Incorporação de aminoácidos activos em oxidação-redução específica do local em proteínas
JP4896745B2 (ja) 2004-02-02 2012-03-14 アンブレツクス・インコーポレイテツド 修飾されたヒトインターフェロンポリペプチドおよびこれらの使用
MXPA06013586A (es) 2004-05-25 2007-11-09 Scripss Res Inst Incorporacion especifica del sitio de aminoacidos no naturales que contienen atomos pesados en proteinas para la determinacion de la estructura cristalina.
EP1771205B1 (de) * 2004-06-18 2016-10-26 Ambrx, Inc. Neuartige antigen-bindende polypeptide und ihre verwendungen
CA2569381A1 (en) * 2004-07-21 2006-08-31 Ambrx, Inc. Biosynthetic polypeptides utilizing non-naturally encoded amino acids
EP2770053B1 (de) 2004-09-21 2018-02-21 The Scripps Research Institute In-vivo-Einbringung von Alkynyl-Aminosäuren in Proteine in Eubakterien
EP1794312A4 (de) * 2004-09-22 2009-08-12 Scripps Research Inst Ortsspezifische etikettierung von proteinen für nmr-studien
JP2008516637A (ja) 2004-10-20 2008-05-22 ザ スクリップス リサーチ インスティテュート 真正細菌へのｎ−アセチルガラクトサミンアミノ酸のインビボ部位特異的組込み
MX2007004892A (es) * 2004-10-27 2007-08-16 Scripps Research Inst Componentes de traduccion ortogonales para la incorporacion in vivo de aminoacidos no naturales.
JP2008035702A (ja) * 2004-11-26 2008-02-21 Institute Of Physical & Chemical Research 蛋白質間相互作用の検出方法
WO2006071840A2 (en) 2004-12-22 2006-07-06 Ambrx, Inc. Formulations of human growth hormone comprising a non-naturally encoded amino acid
GB0428012D0 (en) 2004-12-22 2005-01-26 Hammersmith Imanet Ltd Radiolabelling methods
CA2590429C (en) 2004-12-22 2014-10-07 Ambrx, Inc. Compositions of aminoacyl-trna synthetase and uses thereof
MX2007007580A (es) * 2004-12-22 2007-12-11 Ambrx Inc Hormona del crecimiento humana modificada.
US8080391B2 (en) 2004-12-22 2011-12-20 Ambrx, Inc. Process of producing non-naturally encoded amino acid containing high conjugated to a water soluble polymer
US7385028B2 (en) 2004-12-22 2008-06-10 Ambrx, Inc Derivatization of non-natural amino acids and polypeptides
GB0507123D0 (en) * 2005-04-08 2005-05-11 Isis Innovation Method
CA2609205A1 (en) * 2005-06-03 2006-12-14 Ambrx, Inc. Improved human interferon molecules and their uses
US8586340B2 (en) * 2005-10-12 2013-11-19 The Scripps Research Institute Selective posttranslational modification of phage-displayed polypeptides
US20090018029A1 (en) 2005-11-16 2009-01-15 Ambrx, Inc. Methods and Compositions Comprising Non-Natural Amino Acids
SG170116A1 (en) 2005-12-14 2011-04-29 Ambrx Inc Compositions containing, methods involving, and uses of non-natural amino acids and polypeptides
CN101365807A (zh) 2005-12-22 2009-02-11 加利福尼亚太平洋生物科学股份有限公司 用于掺入核苷酸类似物的聚合酶
WO2007090198A2 (en) * 2006-02-01 2007-08-09 Encode Bio, Inc. Fluorescent assays using orthogonal trna - aminoacyl synthetase pairs
US20070178554A1 (en) * 2006-02-01 2007-08-02 Nima Shiva Orthogonal Aminoacyl Synthetase-tRNA Pairs for Incorporating Unnatural Amino Acids Into Proteins
WO2007092475A2 (en) * 2006-02-06 2007-08-16 Franklin And Marshall College Site-specific incorporation of fluorinated amino acids into proteins
US7960141B2 (en) * 2006-03-09 2011-06-14 The Scripps Research Institute Systems for the expression of orthogonal translation components in eubacterial host cells
JP5249194B2 (ja) 2006-03-16 2013-07-31 ザ スクリプス リサーチ インスティチュート 非天然アミノ酸フェニルセレノシステインを含有するタンパク質の遺伝的にプログラムされた発現
CA2652894A1 (en) * 2006-05-23 2007-12-06 The Scripps Research Institute Genetically encoded fluorescent coumarin amino acids
AU2007292893B2 (en) 2006-09-08 2012-03-01 Ambrx, Inc. Suppressor tRNA transcription in vertebrate cells
AU2007292903B2 (en) 2006-09-08 2012-03-29 Ambrx, Inc. Modified human plasma polypeptide or Fc scaffolds and their uses
CA2662752C (en) * 2006-09-08 2016-04-12 Ambrx, Inc. Site specific incorporation of non-natural amino acids by vertebrate cells
RU2009110152A (ru) 2006-09-21 2010-10-27 Зе Скрипс Ресеч Инститьют (Us) Генетически закодированная формула селективно-сульфатных белков в эубактерии
BRPI0718389A2 (pt) * 2006-10-18 2012-04-10 Scripps Research Inst incorporação genética de aminoácidos antinaturais em proteìnas em células de mamìferos
JP2010523084A (ja) 2007-03-30 2010-07-15 アンブルックス，インコーポレイテッド 修飾ｆｇｆ−２１ポリペプチド
WO2008137471A2 (en) 2007-05-02 2008-11-13 Ambrx, Inc. Modified interferon beta polypeptides and their uses
CA2703736A1 (en) 2007-10-25 2009-04-30 The Scripps Research Institute Genetic incorporation of 3-aminotyrosine into reductases
WO2009061369A2 (en) * 2007-11-02 2009-05-14 The Scripps Research Institute Directed evolution using proteins comprising unnatural amino acids
EP2220218A4 (de) * 2007-11-02 2010-12-08 Scripps Research Inst Genetisch codierte boronat-aminosäure
US8946148B2 (en) 2007-11-20 2015-02-03 Ambrx, Inc. Modified insulin polypeptides and their uses
CN101939410A (zh) * 2007-12-11 2011-01-05 斯克利普斯研究院 甲基营养酵母菌巴斯德毕赤酵母中的体内非天然氨基酸表达
CA2712606A1 (en) 2008-02-08 2009-08-13 Ambrx, Inc. Modified leptin polypeptides and their uses
JP2011514322A (ja) * 2008-02-08 2011-05-06 ザ スクリプス リサーチ インスティチュート 遺伝的にコードされる非天然アミノ酸による免疫寛容解除
AU2009251883B2 (en) 2008-03-31 2014-09-11 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
NZ600382A (en) 2008-07-23 2013-11-29 Ambrx Inc Modified bovine G-CSF polypeptides and their uses
CN102232085A (zh) 2008-09-26 2011-11-02 Ambrx公司 修饰的动物促红细胞生成素多肽和其用途
KR101732054B1 (ko) 2008-09-26 2017-05-02 암브룩스, 인코포레이티드 비천연 아미노산 복제 의존성 미생물 및 백신
US8609383B2 (en) 2008-12-10 2013-12-17 The Scripps Research Institute Production of carrier-peptide conjugates using chemically reactive unnatural amino acids
US20090197339A1 (en) * 2008-12-10 2009-08-06 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris
AU2010341518B2 (en) 2009-12-21 2014-01-09 Ambrx, Inc. Modified porcine somatotropin polypeptides and their uses
JP2013515080A (ja) 2009-12-21 2013-05-02 アンブルックス，インコーポレイテッド 修飾されているウシのソマトトロピンポリペプチドおよびそれらの使用
SG10201506443TA (en) * 2010-08-17 2015-10-29 Ambrx Inc Modified relaxin polypeptides and their uses
US9567386B2 (en) 2010-08-17 2017-02-14 Ambrx, Inc. Therapeutic uses of modified relaxin polypeptides
TWI480288B (zh) 2010-09-23 2015-04-11 Lilly Co Eli 牛顆粒細胞群落刺激因子及其變體之調配物
CA2817218C (en) 2010-11-10 2020-02-18 Nigel L. Webb Nuclions and ribocapsids
WO2012158945A2 (en) * 2011-05-19 2012-11-22 The Scripps Research Institute Compositions and methods for treating charcot-marie-tooth diseases and related neuronal diseases
CN102925427B (zh) * 2011-08-08 2014-01-22 中国科学院生物物理研究所 丙烯酰赖氨酸翻译系统及其应用
SG10201702387YA (en) * 2012-09-24 2017-04-27 Medimmune Ltd Cell lines
CN102925476B (zh) * 2012-11-15 2014-12-03 中国科学院上海巴斯德研究所 酵母中自组装的具有免疫原性的肠道病毒病毒样颗粒的制备方法及其应用
UY35368A (es) 2013-03-08 2014-10-31 Irm Llc Péptidos y composiciones para el tratamiento de daño articular
WO2015009948A1 (en) 2013-07-19 2015-01-22 Regents Of The University Of California Milk fat globule epidermal growth factor 8 regulates fatty acid uptake
CN105723219B (zh) * 2013-09-12 2018-06-05 朗泽科技新西兰有限公司 重组微生物和其使用方法
WO2015048364A1 (en) * 2013-09-27 2015-04-02 President And Fellows Of Harvard College Recombinant cells and organisms having persistent nonstandard amino acid dependence and methods of making them
US10316322B2 (en) 2014-07-02 2019-06-11 Sutro Biopharma, Inc. High growth capacity auxotrophic Escherichia coli and methods of use
CN109295025B (zh) * 2014-09-03 2021-04-02 中国科学院生物物理研究所 酪氨酸类似物翻译系统和基因编码的蛋白质光致电子转移荧光传感器蛋白家族
TWI705071B (zh) 2014-10-24 2020-09-21 美商必治妥美雅史谷比公司 經修飾之fgf-21多肽及其用途
CA3184805A1 (en) 2014-12-04 2016-06-09 Celgene Corporation Biomolecule conjugates
CN108348603B (zh) * 2015-11-03 2023-12-29 Ambrx公司 抗cd3叶酸结合物和其用途
CA3006629A1 (en) * 2015-11-30 2017-06-08 European Molecular Biology Laboratory Means and methods for preparing engineered proteins by genetic code expansion in insect cells
JP2019535707A (ja) 2016-11-14 2019-12-12 ノバルティス アーゲー 軟骨損傷および関節炎の処置のための方法および組成物
JP2020500020A (ja) 2016-11-14 2020-01-09 ノバルティス アーゲー 融合誘導性タンパク質ｍｉｎｉｏｎに関連する組成物、方法、および治療上の使用
MX2019008449A (es) 2017-02-08 2019-09-09 Bristol Myers Squibb Co Polipetidos de relaxina modificada que comprenden un mejorador farmacocinetico y sus usos.
CN107119000B (zh) * 2017-04-19 2019-04-12 山东大学 荧光假单胞菌突变菌株的筛选方法及其在生物防治中的应用
WO2019014267A1 (en) 2017-07-11 2019-01-17 Synthorx, Inc. INCORPORATION OF NON-NATURAL NUCLEOTIDES AND ASSOCIATED METHODS
AU2018309172B2 (en) 2017-08-03 2022-12-15 Synthorx, Inc. Cytokine conjugates for the treatment of autoimmune diseases
MX2020011529A (es) 2018-05-01 2021-01-29 Ambrx Inc Un metodo para optimizar la expresion de anticuerpos.
WO2020132595A1 (en) * 2018-12-21 2020-06-25 The Regents Of The University Of California Il-10-containing vaccines and uses thereof
EP3923974A4 (de) 2019-02-06 2023-02-08 Synthorx, Inc. Il-2-konjugate und verfahren zur verwendung davon
CN110172467B (zh) * 2019-05-24 2021-03-16 浙江大学 一种利用嵌合设计方法构建正交的氨酰-tRNA合成酶/tRNA体系
BR112021025130A2 (pt) * 2019-06-14 2022-03-15 Scripps Research Inst Reagentes e métodos para replicação, transcrição e tradução em organismos semissintéticos
CN114761546A (zh) 2019-10-08 2022-07-15 波士顿学院董事会 含有多个不同非天然氨基酸的蛋白质以及制备和使用此类蛋白质的方法
EP4069842A1 (de) 2019-12-02 2022-10-12 Shape Therapeutics Inc. Therapeutisches editieren
EP4103734A4 (de) * 2020-02-14 2024-10-02 Univ Northwestern Langkettige kohlenstoff- und cyclische aminosäuresubstrate zur genetischen code-umprogrammierung
CN111996206B (zh) * 2020-06-19 2023-01-31 清华大学 光控无细胞蛋白质合成方法、该方法使用的质粒以及使用该方法的产品
CN113481228B (zh) * 2021-06-13 2024-07-19 中国药科大学 微生物非天然氨基酸引入体系及构建方法
WO2023288111A2 (en) 2021-07-16 2023-01-19 Aptah Bio, Inc. Polynucleotide compositions and methods for gene expression regulation
WO2023069816A2 (en) 2021-09-20 2023-04-27 The Broad Institute, Inc. Compositions and methods for multiplex decoding of quadruplet codons
CN115261344B (zh) * 2022-08-29 2023-07-21 北京大学 基于非天然氨基酸的离子液体、其制备方法及应用
WO2024091824A1 (en) 2022-10-26 2024-05-02 Ada Forsyth Institute, Inc. Differentiation and reprogramming of chondrocyte

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE9115660U1 (de) 1991-12-18 1992-07-30 Aventis Research & Technologies GmbH & Co KG, 65929 Frankfurt L-Phenylalanyl-tRNA-Synthetase mit erweiterter Substratselektivität aus Mikroorganismen
US6706504B1 (en) 1996-11-12 2004-03-16 The Scripps Research Institute Tissue type plasminogen activator (t-PA) variants: compositions and methods of use
WO1999061654A1 (en) * 1998-05-27 1999-12-02 The Scripps Research Institute Methods and systems for predicting protein function
US20020061569A1 (en) * 2000-03-21 2002-05-23 Robert Haselbeck Identification of essential genes in prokaryotes
AU2002306849A1 (en) * 2001-03-21 2002-10-08 Elitra Pharmaceuticals, Inc. Identification of essential genes in microorganisms
CA2444098C (en) 2001-04-19 2016-06-21 The Scripps Research Institute Methods and composition for the production of orthogonal trna-aminoacyltrna synthetase pairs
EP1424395B1 (de) 2001-08-01 2011-07-27 Japan Science and Technology Agency Tyrosyl-trna-synthasevarianten
CN1379094A (zh) * 2002-02-01 2002-11-13 杭州华大基因研发中心 耐高温酪氨酰tRNA合成酶基因及其编码的多肽和制备方法
WO2004035605A2 (en) 2002-10-16 2004-04-29 The Scripps Research Institute Glycoprotein synthesis
SI1578949T1 (sl) 2002-10-16 2009-08-31 Scripps Research Inst Usmerjena vključitev keto aminokislin v proteine
CA2508939A1 (en) 2002-12-22 2004-07-15 The Scripps Research Institute Protein arrays
JP5642916B2 (ja) 2003-04-17 2014-12-17 ザ スクリプス リサーチ インスティテュート 真核遺伝コードの拡張
EP2392923B1 (de) 2003-06-18 2014-05-21 The Scripps Research Institute Methode zur Herstellung von Proteinen mit unnatürlichen Aminosäuren
ES2301099T3 (es) 2003-07-07 2008-06-16 The Scripps Research Institute Composiciones de parejas ortogonales lisil-arnt y aminoacil-arnt sintetasa y usos de las mismas.
WO2005007870A2 (en) 2003-07-07 2005-01-27 The Scripps Research Institute COMPOSITIONS OF ORTHOGONAL LEUCYL-tRNA AND AMINOACYL-tRNA SYNTHETASE PAIRS AND USES THEREOF
EP1658366A4 (de) 2003-07-07 2006-08-09 Scripps Research Inst Zusammensetzungen von orthogonalen glutamyl-trna- und aminoacyl-trna-synthetasepaaren und deren verwendungen
PT1687401E (pt) 2003-10-14 2013-02-26 Scripps Research Inst Incorporação de aminoácidos activos em oxidação-redução específica do local em proteínas
US7709604B2 (en) 2003-12-18 2010-05-04 The Scripps Research Institute Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells
MXPA06013586A (es) 2004-05-25 2007-11-09 Scripss Res Inst Incorporacion especifica del sitio de aminoacidos no naturales que contienen atomos pesados en proteinas para la determinacion de la estructura cristalina.
US20090181858A1 (en) 2004-09-21 2009-07-16 The Scripps Research Institute Adding photoregulated amino acids to the genetic code
EP2770053B1 (de) 2004-09-21 2018-02-21 The Scripps Research Institute In-vivo-Einbringung von Alkynyl-Aminosäuren in Proteine in Eubakterien
EP1794312A4 (de) 2004-09-22 2009-08-12 Scripps Research Inst Ortsspezifische etikettierung von proteinen für nmr-studien
JP2008516637A (ja) 2004-10-20 2008-05-22 ザ スクリップス リサーチ インスティテュート 真正細菌へのｎ−アセチルガラクトサミンアミノ酸のインビボ部位特異的組込み
MX2007004892A (es) 2004-10-27 2007-08-16 Scripps Research Inst Componentes de traduccion ortogonales para la incorporacion in vivo de aminoacidos no naturales.
US8586340B2 (en) 2005-10-12 2013-11-19 The Scripps Research Institute Selective posttranslational modification of phage-displayed polypeptides
US7960141B2 (en) 2006-03-09 2011-06-14 The Scripps Research Institute Systems for the expression of orthogonal translation components in eubacterial host cells
JP5249194B2 (ja) 2006-03-16 2013-07-31 ザ スクリプス リサーチ インスティチュート 非天然アミノ酸フェニルセレノシステインを含有するタンパク質の遺伝的にプログラムされた発現
CA2652894A1 (en) 2006-05-23 2007-12-06 The Scripps Research Institute Genetically encoded fluorescent coumarin amino acids
RU2009110152A (ru) 2006-09-21 2010-10-27 Зе Скрипс Ресеч Инститьют (Us) Генетически закодированная формула селективно-сульфатных белков в эубактерии
BRPI0718389A2 (pt) 2006-10-18 2012-04-10 Scripps Research Inst incorporação genética de aminoácidos antinaturais em proteìnas em células de mamìferos
CA2703736A1 (en) 2007-10-25 2009-04-30 The Scripps Research Institute Genetic incorporation of 3-aminotyrosine into reductases
WO2009061369A2 (en) 2007-11-02 2009-05-14 The Scripps Research Institute Directed evolution using proteins comprising unnatural amino acids
EP2220218A4 (de) 2007-11-02 2010-12-08 Scripps Research Inst Genetisch codierte boronat-aminosäure
WO2009064416A2 (en) 2007-11-15 2009-05-22 The Scripps Research Institute Genetic incorporation of an alpha-hydroxy acid into proteins to generate ester backbone linkages at defined sites
JP2011514322A (ja) 2008-02-08 2011-05-06 ザ スクリプス リサーチ インスティチュート 遺伝的にコードされる非天然アミノ酸による免疫寛容解除
US20110076718A1 (en) 2008-02-27 2011-03-31 The Scripps Research Institute In vivo incorporation of an unnatural amino acid comprising a 1,2-aminothiol group
US20090197339A1 (en) 2008-12-10 2009-08-06 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris
US8609383B2 (en) 2008-12-10 2013-12-17 The Scripps Research Institute Production of carrier-peptide conjugates using chemically reactive unnatural amino acids
WO2010114615A2 (en) 2009-04-03 2010-10-07 The Scripps Research Institute A facile system for encoding unnatural amino acids in mammalian cells

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