ATE532861T1 - Expressionsvektor - Google Patents

Expressionsvektor

Info

Publication number
ATE532861T1
ATE532861T1 AT04783263T AT04783263T ATE532861T1 AT E532861 T1 ATE532861 T1 AT E532861T1 AT 04783263 T AT04783263 T AT 04783263T AT 04783263 T AT04783263 T AT 04783263T AT E532861 T1 ATE532861 T1 AT E532861T1
Authority
AT
Austria
Prior art keywords
expression vector
expression
nucleic acids
host cells
desired polypeptide
Prior art date
Application number
AT04783263T
Other languages
English (en)
Inventor
Amelia Black
Original Assignee
Medarex Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medarex Inc filed Critical Medarex Inc
Application granted granted Critical
Publication of ATE532861T1 publication Critical patent/ATE532861T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C07K14/805—Haemoglobins; Myoglobins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/15—Vector systems having a special element relevant for transcription chimeric enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT04783263T 2003-09-04 2004-09-03 Expressionsvektor ATE532861T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US50080303P 2003-09-04 2003-09-04
PCT/US2004/028954 WO2005024015A1 (en) 2003-09-04 2004-09-03 Expression vector

Publications (1)

Publication Number Publication Date
ATE532861T1 true ATE532861T1 (de) 2011-11-15

Family

ID=34272998

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04783263T ATE532861T1 (de) 2003-09-04 2004-09-03 Expressionsvektor

Country Status (7)

Country Link
US (1) US7674618B2 (de)
EP (1) EP1660654B1 (de)
JP (1) JP4751326B2 (de)
AT (1) ATE532861T1 (de)
CA (1) CA2536807C (de)
ES (1) ES2374526T3 (de)
WO (1) WO2005024015A1 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2007235496B2 (en) 2006-03-31 2013-11-21 E. R. Squibb & Sons, L.L.C. Transgenic animals expressing chimeric antibodies for use in preparing human antibodies
ME02269B (me) 2006-10-02 2016-04-28 Medarex Inc Humana antitela koja se vezuju za cxcr4 i njihove upotrebe
KR20150067395A (ko) 2006-12-01 2015-06-17 메다렉스, 엘.엘.시. 씨디22에 결합하는 인간 항체 및 이의 용도
RU2456346C2 (ru) * 2007-05-02 2012-07-20 Мериал Лимитед Днк-плазмиды, обладающие повышенной экспрессией и стабильностью
KR101445903B1 (ko) * 2007-05-02 2014-09-29 메리얼 리미티드 향상된 발현 및 안정성을 갖는 ｄｎａ 플라스미드
CA2705925C (en) * 2007-12-19 2019-03-12 Glycofi, Inc. Yeast strains for protein production
EP2227546B1 (de) * 2007-12-21 2016-05-04 Novartis AG Säuger-expressionsvektor
AR072999A1 (es) 2008-08-11 2010-10-06 Medarex Inc Anticuerpos humanos que se unen al gen 3 de activacion linfocitaria (lag-3) y los usos de estos
JP4547643B1 (ja) * 2009-06-05 2010-09-22 東洋紡績株式会社 クローニングに最適化された発現ベクター
JP4525863B1 (ja) 2009-06-05 2010-08-18 東洋紡績株式会社 高生産性細胞の樹立のための発現ベクター及び高生産性細胞
JP4491808B1 (ja) * 2009-06-05 2010-06-30 東洋紡績株式会社 高発現細胞の効率的な選別に有用な薬剤選択マーカー遺伝子発現カセット
KR102024957B1 (ko) 2011-11-09 2019-09-24 브리스톨-마이어스 스큅 컴퍼니 항-cxcr4 항체를 이용한 혈액 악성종양의 치료
IL300591A (en) * 2016-05-11 2023-04-01 Amgen Inc Direct selection of cells expressing high levels of heteromeric proteins using intergenic glutamine synthetase complementation vectors
BR112021010785A2 (pt) * 2018-12-04 2021-11-16 Catalent Pharma Solutions Llc Vetores para fabricação de proteínas

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4713339A (en) 1983-01-19 1987-12-15 Genentech, Inc. Polycistronic expression vector construction
US4876197A (en) 1983-02-22 1989-10-24 Chiron Corporation Eukaryotic regulatable transcription
EP0377842B1 (de) 1988-12-13 1995-10-04 President And Fellows Of Harvard College Prototypische FeLV-Isolate für die Verwendung als Krankheitsmuster oder Impfstoffe
US5256568A (en) * 1990-02-12 1993-10-26 Regeneron Phamaceuticals, Inc. Vectors and transformed most cells for recombinant protein production with reduced expression of selectable markers
WO1992001800A1 (en) 1990-07-20 1992-02-06 Chiron Corporation Method for integrative transformation of yeast using dispersed repetitive elements
US5648267A (en) * 1992-11-13 1997-07-15 Idec Pharmaceuticals Corporation Impaired dominant selectable marker sequence and intronic insertion strategies for enhancement of expression of gene product and expression vector systems comprising same
EP0669986B1 (de) 1992-11-13 2003-04-09 Idec Pharmaceuticals Corporation Konsensus-kozak-sequenzen zur säugetier-exprimierung
US5627033A (en) * 1995-03-28 1997-05-06 Research & Diagnostics Systems, Inc. Mammalian expression vectors
US5928941A (en) * 1996-10-07 1999-07-27 President And Fellows Of Harvard College Repressor kruppel-like factor
ID24565A (id) * 1997-03-14 2000-07-27 Idec Pharma Corp Metoda untuk integrasi gen pada tempat yang spesifik dalam sel mamalia melalui rekombinasi homolog dan vektor untuk pencapaian yang sama
US6444421B1 (en) * 1997-11-19 2002-09-03 The United States Of America As Represented By The Department Of Health And Human Services Methods for detecting intermolecular interactions in vivo and in vitro
US6316253B1 (en) 1999-11-01 2001-11-13 Chiron Corporation Expression vectors, transfection systems, and method of use thereof
US20040029229A1 (en) * 2002-05-20 2004-02-12 Reeves Philip J. High level protein expression system

Also Published As

Publication number Publication date
WO2005024015A1 (en) 2005-03-17
EP1660654B1 (de) 2011-11-09
JP4751326B2 (ja) 2011-08-17
US20050153394A1 (en) 2005-07-14
ES2374526T3 (es) 2012-02-17
CA2536807C (en) 2010-07-13
EP1660654A1 (de) 2006-05-31
CA2536807A1 (en) 2005-03-17
JP2007503837A (ja) 2007-03-01
EP1660654A4 (de) 2007-08-22
US7674618B2 (en) 2010-03-09

Similar Documents

Publication Publication Date Title
PH12020550771A1 (en) Gene editing using a modified closed-ended dna (cedna)
WO2020028555A3 (en) Novel crispr enzymes and systems
ATE532861T1 (de) Expressionsvektor
WO2003080807A3 (en) Compositions and methods for suppressing eukaryotic gene expression
EP4431607A3 (de) Hochdurchsatz-präzisionsgenomeditierung
WO2004099370A3 (en) NOVEL BACILLUS mHKcel CELLULASE
DK1928901T3 (da) Polypeptider, der har beta-glucosidaseaktivitet og polynukleotider, der koder for samme
ATE342917T1 (de) Antimikrobielle polypeptide aus pseudoplectania nigrella
WO2020219682A3 (en) Gene knock-outs to improve t cell function
CY1116413T1 (el) Καινοφανη πληρως-ανθρωπινα μονοκλωνικα αντισωματα anti-vap-1
DK1675956T3 (da) Minimal DNA-sekvens, der virksom som chromatinisolator, og dennes anvendelse til proteinekspression
DK1685247T3 (da) Vektorer til retningsbestemt kloning
WO2004097001A3 (en) Novel bacillus 029cel cellulase
WO2007134325A3 (en) Methods and compositions for protein production using adenoviral vectors
SG142300A1 (en) Promoters for expression in modified vaccinia virus ankara
WO2005034876A3 (en) Use of cell lines to produce active therapeutic proteins
WO2007051063A3 (en) Gpcr expressing cell lines and antibodies
TW200621284A (en) Polypeptides having antimicrobial activity and polynucleotides encoding same
TW200510460A (en) Antimicrobial polypeptides
DK1756273T3 (da) Signalpeptid til frembringelse af et polypeptid
TW200734350A (en) Polypeptides having antimicrobial activity and polynucleotides encoding same
TW200502253A (en) Antimicrobial polypeptides
DK2066790T3 (da) Galactosyltransferase
ATE466874T1 (de) Mit scytovirin der domäne 1 verwandte polypeptide
WO2004087886A3 (en) Modified adenoviral e1a constructs and methods of use thereof