DK1685247T3 - Vektorer til retningsbestemt kloning - Google Patents

Vektorer til retningsbestemt kloning

Info

Publication number
DK1685247T3
DK1685247T3 DK04821603T DK04821603T DK1685247T3 DK 1685247 T3 DK1685247 T3 DK 1685247T3 DK 04821603 T DK04821603 T DK 04821603T DK 04821603 T DK04821603 T DK 04821603T DK 1685247 T3 DK1685247 T3 DK 1685247T3
Authority
DK
Denmark
Prior art keywords
vectors
directional cloning
cloning
directional
present
Prior art date
Application number
DK04821603T
Other languages
English (en)
Inventor
Michael R Slater
Ethan Edward Strauss
Keith V Wood
James Robert Hartnett
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp filed Critical Promega Corp
Priority claimed from PCT/US2004/031912 external-priority patent/WO2005087932A2/en
Application granted granted Critical
Publication of DK1685247T3 publication Critical patent/DK1685247T3/da

Links

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • C12N15/73Expression systems using phage (lambda) regulatory sequences
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B50/00ICT programming tools or database systems specially adapted for bioinformatics
    • G16B50/30Data warehousing; Computing architectures
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B50/00ICT programming tools or database systems specially adapted for bioinformatics

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Theoretical Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Medical Informatics (AREA)
  • Evolutionary Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Databases & Information Systems (AREA)
  • Bioethics (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
  • Catalysts (AREA)
  • Image Analysis (AREA)
  • Radar Systems Or Details Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Magnetic Resonance Imaging Apparatus (AREA)
DK04821603T 2003-10-03 2004-09-29 Vektorer til retningsbestemt kloning DK1685247T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10/678,961 US8293503B2 (en) 2003-10-03 2003-10-03 Vectors for directional cloning
US10/702,228 US20050074785A1 (en) 2003-10-03 2003-11-05 Vectors for directional cloning
PCT/US2004/031912 WO2005087932A2 (en) 2003-10-03 2004-09-29 Vectors for directional cloning

Publications (1)

Publication Number Publication Date
DK1685247T3 true DK1685247T3 (da) 2010-03-08

Family

ID=34394063

Family Applications (2)

Application Number Title Priority Date Filing Date
DK04821603T DK1685247T3 (da) 2003-10-03 2004-09-29 Vektorer til retningsbestemt kloning
DK07013756T DK1847611T3 (da) 2003-10-03 2004-09-29 Rhamnose-inducerbart ekspressionssystem

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK07013756T DK1847611T3 (da) 2003-10-03 2004-09-29 Rhamnose-inducerbart ekspressionssystem

Country Status (6)

Country Link
US (7) US8293503B2 (da)
EP (1) EP2116601B1 (da)
JP (3) JP5456706B2 (da)
AT (2) ATE539156T1 (da)
DE (2) DE602004024107D1 (da)
DK (2) DK1685247T3 (da)

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US11618899B2 (en) 2016-02-09 2023-04-04 Brookhaven Science Associates, Llc Cloning and expression vectors and systems
CN106222186B (zh) * 2016-08-22 2019-03-19 河北省农林科学院粮油作物研究所 入门载体pAmpGate和pGentGate的构建及使用方法
US10435695B2 (en) 2016-09-08 2019-10-08 The Government of the United States of America, as represented by the Secretary of Homeland Security Fusion protein comprising Gaussia luciferase, translation interrupter sequence, and interferon amino acid sequences
US10385319B2 (en) 2016-09-08 2019-08-20 The Governement of the United States of America, as represented by the Secretary of Homeland Security Modified foot-and-mouth disease virus 3C proteases, compositions and methods thereof
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
KR20210125494A (ko) * 2019-01-09 2021-10-18 아시모브 인코포레이티드 다중핵산 벡터의 조성물 및 이의 용도
CN111235638A (zh) * 2020-02-20 2020-06-05 北京良芯生物科技发展有限公司 一种构建测序文库的方法及试剂盒
CN115698271A (zh) * 2020-06-12 2023-02-03 南京金斯瑞生物科技有限公司 含barstar基因的工程菌及其在barnase基因克隆中的应用
EP3981879A1 (en) * 2020-10-09 2022-04-13 Stada Arzneimittel Ag Method of producing a recombinant protein in a host cell which has a disabled rhamnose metabolism as well as expression vectors, host cells and recombinant proteins thereof

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Also Published As

Publication number Publication date
JP5456706B2 (ja) 2014-04-02
US9469857B2 (en) 2016-10-18
JP6341941B2 (ja) 2018-06-13
US9371531B2 (en) 2016-06-21
EP2116601A2 (en) 2009-11-11
ATE448303T1 (de) 2009-11-15
US8293503B2 (en) 2012-10-23
ATE539156T1 (de) 2012-01-15
DE602004024107D1 (de) 2009-12-24
US20140186959A1 (en) 2014-07-03
EP2116601B1 (en) 2011-12-28
US20050074883A1 (en) 2005-04-07
US9018014B2 (en) 2015-04-28
US20130183760A1 (en) 2013-07-18
JP2013247960A (ja) 2013-12-12
DE602004023140D1 (de) 2009-10-22
US20050074785A1 (en) 2005-04-07
EP2116601A3 (en) 2010-06-09
US20050130205A1 (en) 2005-06-16
US20100216231A1 (en) 2010-08-26
US8367403B2 (en) 2013-02-05
DK1847611T3 (da) 2010-01-04
JP2016052338A (ja) 2016-04-14
US20130164837A1 (en) 2013-06-27
JP5899168B2 (ja) 2016-04-06
JP2011083296A (ja) 2011-04-28

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