CN115698271A - 含barstar基因的工程菌及其在barnase基因克隆中的应用 - Google Patents

含barstar基因的工程菌及其在barnase基因克隆中的应用 Download PDF

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Publication number
CN115698271A
CN115698271A CN202180041549.3A CN202180041549A CN115698271A CN 115698271 A CN115698271 A CN 115698271A CN 202180041549 A CN202180041549 A CN 202180041549A CN 115698271 A CN115698271 A CN 115698271A
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gene
sequence
barstar
plasmid
engineered bacterium
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CN202180041549.3A
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陈康明
周斌
毛灵琪
孙迎娣
孟庆伟
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Nanjing Jinsirui Science and Technology Biology Corp
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Nanjing Jinsirui Science and Technology Biology Corp
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material

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Abstract

提供了含barstar基因的工程菌及其在barnase基因克隆中的应用，其中，所述工程菌的染色体基因组中包含barstar基因，所述工程菌能用于克隆barnase基因。还提供了所述barnase基因的克隆方法，包括将包含barnase基因的质粒转化到上述的工程菌中培养扩增。

Description

PCT国内申请，说明书已公开。

Claims (25)

  1. PCT国内申请，权利要求书已公开。
CN202180041549.3A 2020-06-12 2021-06-11 含barstar基因的工程菌及其在barnase基因克隆中的应用 Pending CN115698271A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN202010534573 2020-06-12
CN2020105345738 2020-06-12
PCT/CN2021/099659 WO2021249536A1 (zh) 2020-06-12 2021-06-11 含barstar基因的工程菌及其在barnase基因克隆中的应用

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CN115698271A true CN115698271A (zh) 2023-02-03

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CN202180041549.3A Pending CN115698271A (zh) 2020-06-12 2021-06-11 含barstar基因的工程菌及其在barnase基因克隆中的应用

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CN (1) CN115698271A (zh)
WO (1) WO2021249536A1 (zh)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8293503B2 (en) * 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
WO2014187311A1 (zh) * 2013-05-23 2014-11-27 深圳市作物分子设计育种研究院 一种使植物花粉特异性失活的载体及其用途

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