WO2009011258A1 - 外来遺伝子を細胞に安定に保持する方法 - Google Patents

外来遺伝子を細胞に安定に保持する方法 Download PDF

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Publication number
WO2009011258A1
WO2009011258A1 PCT/JP2008/062391 JP2008062391W WO2009011258A1 WO 2009011258 A1 WO2009011258 A1 WO 2009011258A1 JP 2008062391 W JP2008062391 W JP 2008062391W WO 2009011258 A1 WO2009011258 A1 WO 2009011258A1
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WO
WIPO (PCT)
Prior art keywords
foreign gene
gene
protein
host cell
peptide encoded
Prior art date
Application number
PCT/JP2008/062391
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English (en)
French (fr)
Inventor
Yuji Hatada
Yukari Ohta
Yuko Hidaka
Nobuyuki Nakamura
Original Assignee
Japan Agency For Marine-Earth Science And Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Agency For Marine-Earth Science And Technology filed Critical Japan Agency For Marine-Earth Science And Technology
Priority to CN200880024589A priority Critical patent/CN101849002A/zh
Priority to US12/452,645 priority patent/US20110008832A1/en
Priority to EP08778000A priority patent/EP2177610A4/en
Publication of WO2009011258A1 publication Critical patent/WO2009011258A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)

Abstract

　本発明は、任意の外来遺伝子を発現可能な状態で挿入し、アミノアシルｔＲＮＡ合成酵素をコードする遺伝子を発現可能な状態で有する組換えベクターを用意する工程、アミノアシルｔＲＮＡ合成酵素をコードする染色体遺伝子を欠損させた変異宿主細胞等を用意する工程、変異宿主細胞を組換えベクターで形質転換して形質転換体を得る工程、形質転換体を培養して、前記外来遺伝子がコードするタンパク質またはペプチドを調製する工程を含む、外来遺伝子を発現させて前記外来遺伝子がコードするタンパク質またはペプチドを調製する方法に関する。本発明によれば、抗生物質を用いず、培地組成に制約されずに組換えＤＮＡクローニングベクターを宿主細胞中に維持し得る新たな手段とこの手段を利用した外来遺伝子がコードするタンパク質またはペプチドを調製する方法を提供することができる。
PCT/JP2008/062391 2007-07-13 2008-07-09 外来遺伝子を細胞に安定に保持する方法 WO2009011258A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CN200880024589A CN101849002A (zh) 2007-07-13 2008-07-09 在细胞中稳定保持外来基因的方法
US12/452,645 US20110008832A1 (en) 2007-07-13 2008-07-09 Methods for stably retaining foreign genes in cells
EP08778000A EP2177610A4 (en) 2007-07-13 2008-07-09 METHOD FOR STABLY PRESERVING A FOREIGN GENE IN A CELL

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-183931 2007-07-13
JP2007183931A JP2009017840A (ja) 2007-07-13 2007-07-13 外来遺伝子を細胞に安定に保持する方法

Publications (1)

Publication Number Publication Date
WO2009011258A1 true WO2009011258A1 (ja) 2009-01-22

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Family Applications (1)

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PCT/JP2008/062391 WO2009011258A1 (ja) 2007-07-13 2008-07-09 外来遺伝子を細胞に安定に保持する方法

Country Status (5)

Country Link
US (1) US20110008832A1 (ja)
EP (1) EP2177610A4 (ja)
JP (1) JP2009017840A (ja)
CN (1) CN101849002A (ja)
WO (1) WO2009011258A1 (ja)

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CN108165537A (zh) * 2010-04-27 2018-06-15 Atyr医药公司 与苏氨酰-tRNA合成酶的蛋白片段相关的治疗、诊断和抗体组合物的创新发现

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AU2011248625B2 (en) 2010-04-26 2017-01-05 Pangu Biopharma Limited Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of cysteinyl-tRNA synthetase
JP6294074B2 (ja) 2010-04-27 2018-03-14 エータイアー ファーマ， インコーポレイテッド イソロイシルｔＲＮＡ合成酵素のタンパク質フラグメントに関連した治療用、診断用および抗体組成物の革新的発見
CA2797271C (en) 2010-04-28 2021-05-25 Atyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of alanyl trna synthetases
CN103097523B (zh) 2010-04-29 2016-09-28 Atyr医药公司 与天冬酰胺酰-tRNA合成酶的蛋白片段相关的治疗、诊断和抗体组合物的创新发现
CA2797393C (en) 2010-04-29 2020-03-10 Atyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of valyl trna synthetases
ES2668207T3 (es) 2010-05-03 2018-05-17 Atyr Pharma, Inc. Descubrimiento innovador de composiciones terapéuticas, de diagnóstico y de anticuerpos relacionadas con fragmentos de proteínas de metionil-ARNt sintetasas
JP6039546B2 (ja) 2010-05-03 2016-12-07 エータイアー ファーマ， インコーポレイテッド セリルｔＲＮＡ合成酵素のタンパク質フラグメントに関連した治療用、診断用および抗体組成物の革新的発見
CN103096925A (zh) 2010-05-03 2013-05-08 Atyr医药公司 与精氨酰-tRNA合成酶的蛋白片段相关的治疗、诊断和抗体组合物的创新发现
AU2011248227B2 (en) 2010-05-03 2016-12-01 Pangu Biopharma Limited Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of phenylalanyl-alpha-tRNA synthetases
CN102985103A (zh) 2010-05-04 2013-03-20 Atyr医药公司 与p38多-tRNA合成酶复合物相关的治疗、诊断和抗体组合物的创新发现
CN103096909A (zh) 2010-05-04 2013-05-08 Atyr医药公司 与谷氨酰-脯氨酰-tRNA合成酶的蛋白片段相关的治疗、诊断和抗体组合物的创新发现
US8945541B2 (en) 2010-05-14 2015-02-03 Atyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of phenylalanyl-beta-tRNA synthetases
CA2799480C (en) 2010-05-17 2020-12-15 Atyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of leucyl-trna synthetases
EP2575856B1 (en) 2010-05-27 2017-08-16 aTyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of glutaminyl-trna synthetases
EP2575857B1 (en) 2010-06-01 2018-01-24 aTyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of lysyl-trna synthetases
CA2804278C (en) 2010-07-12 2021-07-13 Atyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of aspartyl-trna synthetases
JP6177129B2 (ja) 2010-07-12 2017-08-09 エータイアー ファーマ， インコーポレイテッド ヒスチジルｔＲＮＡ合成酵素のタンパク質フラグメントに関連した治療用、診断用および抗体組成物の革新的発見
WO2012021247A2 (en) 2010-07-12 2012-02-16 Atyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of glycyl-trna synthetases
US8999321B2 (en) 2010-07-12 2015-04-07 Atyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of glycyl-tRNA synthetases
CA2808539C (en) 2010-08-25 2021-05-25 Atyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of tyrosyl-trna synthetases
US9399770B2 (en) 2010-10-06 2016-07-26 Atyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of tryptophanyl-tRNA synthetases
US9714419B2 (en) 2011-08-09 2017-07-25 Atyr Pharma, Inc. PEGylated tyrosyl-tRNA synthetase polypeptides
WO2013086216A1 (en) 2011-12-06 2013-06-13 Atyr Pharma, Inc. Improved aspartyl-trna synthetases
WO2013086228A1 (en) 2011-12-06 2013-06-13 Atyr Pharma, Inc. Pegylated aspartyl-trna synthetase polypeptides
AU2012368189B2 (en) 2011-12-29 2017-08-31 Atyr Pharma, Inc. Aspartyl-tRNA synthetase-Fc conjugates
ES2708565T3 (es) 2013-03-15 2019-04-10 Atyr Pharma Inc Conjugados de Fc-histidil-ARNt sintetasa
US10837006B2 (en) * 2016-01-21 2020-11-17 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
WO2018195338A1 (en) 2017-04-20 2018-10-25 Atyr Pharma, Inc. Compositions and methods for treating lung inflammation
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
CN110172467B (zh) * 2019-05-24 2021-03-16 浙江大学 一种利用嵌合设计方法构建正交的氨酰-tRNA合成酶/tRNA体系
WO2023085429A1 (ja) * 2021-11-15 2023-05-19 合同酒精株式会社 形質転換細胞及び目的タンパク質の生産方法

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108165537A (zh) * 2010-04-27 2018-06-15 Atyr医药公司 与苏氨酰-tRNA合成酶的蛋白片段相关的治疗、诊断和抗体组合物的创新发现

Also Published As

Publication number Publication date
EP2177610A1 (en) 2010-04-21
EP2177610A4 (en) 2011-05-18
CN101849002A (zh) 2010-09-29
US20110008832A1 (en) 2011-01-13
JP2009017840A (ja) 2009-01-29

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