ES2061482T3 - Metodo para la identificacion de secuencias de señal secretora para sintesis de proteina extracelular en bacillus. - Google Patents

Metodo para la identificacion de secuencias de señal secretora para sintesis de proteina extracelular en bacillus.

Info

Publication number
ES2061482T3
ES2061482T3 ES87200813T ES87200813T ES2061482T3 ES 2061482 T3 ES2061482 T3 ES 2061482T3 ES 87200813 T ES87200813 T ES 87200813T ES 87200813 T ES87200813 T ES 87200813T ES 2061482 T3 ES2061482 T3 ES 2061482T3
Authority
ES
Spain
Prior art keywords
sequences
bacillus
vectors
secretion
signal sequences
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES87200813T
Other languages
English (en)
Inventor
Hilde E Smith
Ee Jan Hendrik Van
Ben P H Peeters
Sierd Bron
Gerard Venema
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gist Brocades NV
Original Assignee
Gist Brocades NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gist Brocades NV filed Critical Gist Brocades NV
Application granted granted Critical
Publication of ES2061482T3 publication Critical patent/ES2061482T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

LA INVENCION PROPORCIONA NUEVOS VECTORES PARA IDENTIFICAR SECUENCIAS DE LA SEÑAL SECRETORA DE FRAGMENTOS DE ADN DE MICROORGANISMOS UNICELULARES. LOS PLASMIDOS COMPRENDEN UN SITIO DE CLONACION MULTIPLE CON SITIOS DE RESTRICCION EN EL CUADRO DE LECTURA, CON UN GEN ESTRUCTURAL QUE PERMITE LA RELACION RAPIDA DEL PRODUCTO DE EXPRESION SECRETADO. OPCIONALMENTE, LOS VECTORES PUEDE INCLUIR UNA REGION PROMOTORA SITUADA ANTES DEL SITIO DE CLONACION MULTIPLE. LA INVENCION SE ILUSTRA CON EL EJEMPLO DE BACILLUS. SE HAN AISLADO SECUENCIAS DE SEÑALES SECRETORAS ESPECIFICAS CON AQUELLOS VECTORES, QUE PERMITEN SECRECION REAL DE PROTEINAS CON RENDIMIENTOS ECONOMICAMENTE ALTOS. ALGUNAS SECUENCIAS DE SEÑALES AISLADAS PARECEN SER MUCHO MEJORES QUE LAS SECUENCIA QUE REGULAN LA SECRECION DE ALTO RENDIMIENTO DE PROTEINAS EXTRACELULARES CONOCIDAS.
ES87200813T 1986-05-02 1987-04-29 Metodo para la identificacion de secuencias de señal secretora para sintesis de proteina extracelular en bacillus. Expired - Lifetime ES2061482T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP86200774 1986-05-02

Publications (1)

Publication Number Publication Date
ES2061482T3 true ES2061482T3 (es) 1994-12-16

Family

ID=8195736

Family Applications (1)

Application Number Title Priority Date Filing Date
ES87200813T Expired - Lifetime ES2061482T3 (es) 1986-05-02 1987-04-29 Metodo para la identificacion de secuencias de señal secretora para sintesis de proteina extracelular en bacillus.

Country Status (10)

Country Link
US (1) US5037760A (es)
EP (1) EP0244042B1 (es)
JP (1) JPS6339586A (es)
AT (1) ATE110111T1 (es)
DE (1) DE3750378T2 (es)
DK (1) DK221087A (es)
ES (1) ES2061482T3 (es)
FI (1) FI871919A (es)
IE (1) IE64916B1 (es)
PT (1) PT84801B (es)

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6238889B1 (en) 1986-12-16 2001-05-29 Dsm N.V. Molecular cloning and expression of the Pro8 isoform of human IL-3
US5304637A (en) * 1987-07-13 1994-04-19 Gist-Brocades N.V. Expression and purification of human interleukin-3 and muteins thereof
US6384194B1 (en) 1987-12-16 2002-05-07 Dsm N.V. Expression and purification of human interleukin-3 and muteins thereof
US5242821A (en) * 1989-07-10 1993-09-07 Valio, Finnish Co-Operative Dairies' Association Lactococcus promoter and signal sequences for expression in bacteria
DK0574402T3 (da) 1990-11-26 1998-05-18 Chiron Corp Ekspression af PACE i værtsceller og fremgangsmåder til anvendelse deraf
US5422427A (en) * 1991-09-17 1995-06-06 The United States Of America As Represented By The United States Department Of Health And Human Services Pneumococcal fimbrial protein A
US5854416A (en) * 1991-11-14 1998-12-29 The United States Of America As Represented By The Department Of Health And Human Services Streptococcus pneumoniae 37-KDA surface adhesin a protein and nucleic acids coding therefor
US6312944B1 (en) 1991-11-14 2001-11-06 The United States Of America As Represented By The Department Of Health And Human Services Pneumococcal fimbrial protein A
GB9513371D0 (en) 1995-06-30 1995-09-06 Biocine Spa Immunogenic detoxified mutant toxins
JP2879303B2 (ja) * 1993-01-14 1999-04-05 佑 本庶 ｃＤＮＡライブラリーの作製方法、および新規なポリペプチドとそれをコードするＤＮＡ
US5536637A (en) * 1993-04-07 1996-07-16 Genetics Institute, Inc. Method of screening for cDNA encoding novel secreted mammalian proteins in yeast
US5629167A (en) 1994-04-19 1997-05-13 Biocine S.P.A. Detection of antibodies against Chlamydia trachomatis pgp3 antigen in patient sera by enzyme-linked immunosorbent assay
US5789653A (en) * 1995-01-10 1998-08-04 University Of Edinburgh Secretory gene trap
AU6045196A (en) * 1995-06-07 1996-12-30 Zymogenetics Inc. Secretion leader trap cloning method
US5753462A (en) * 1995-06-07 1998-05-19 Zymogenetics, Inc. Secretion leader trap cloning method
FR2739629B1 (fr) * 1995-10-06 1997-12-26 Systems Bio Ind Utilisation d'un systeme de secretion sec-dependant pour secreter des proteines normalement secretees par un systeme de secretion sec-independant, bacteries les contenant et leur utilisation
CA2252425A1 (en) * 1996-04-24 1997-10-30 Genetics Institute, Inc. Yeast invertase gene as reporter system for isolating cytokines
US6136569A (en) * 1997-02-27 2000-10-24 Genentech, Inc. Method of creating a cDNA library enriched in signal sequences
GB9719637D0 (en) * 1997-09-15 1997-11-19 Genencor Int Bv Proteases from gram-positive organisms
US6300117B1 (en) * 1997-09-15 2001-10-09 Genencor International, Inc. Proteases from gram-positive organisms
US6521440B1 (en) 1997-09-15 2003-02-18 Genencor International, Inc. Proteases from gram-positive organisms
BR9813930A (pt) 1997-11-06 2006-12-19 Chiron Spa antìgeno neisserial
WO1999036544A2 (en) 1998-01-14 1999-07-22 Chiron S.P.A. Neisseria meningitidis antigens
US6150098A (en) * 1998-02-20 2000-11-21 Amgen Inc. Methods for identifying novel secreted mammalian polypeptides
US6103472A (en) * 1998-02-20 2000-08-15 Amgen Inc. Methods and compositions for identifying novel secreted mammalian polypeptides in yeast
CA2326408A1 (en) * 1998-03-02 1999-09-10 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Epitope peptides immunogenic against streptococcus pneumoniae
AU3193399A (en) 1998-03-23 1999-10-18 Genentech Inc. Method of selection for genes encoding secreted and transmembrane proteins
GB9808932D0 (en) 1998-04-27 1998-06-24 Chiron Spa Polyepitope carrier protein
WO2001031019A2 (en) 1999-10-29 2001-05-03 Chiron Spa Neisserial antigenic peptides
JP5102414B2 (ja) 1998-05-01 2012-12-19 ノバルティス バクシンズ アンド ダイアグノスティックス，インコーポレーテッド 髄膜炎菌抗原および組成物
CN100392082C (zh) 1999-04-30 2008-06-04 启龙股份公司 保守的奈瑟球菌抗原
GB9911683D0 (en) 1999-05-19 1999-07-21 Chiron Spa Antigenic peptides
GB9916529D0 (en) 1999-07-14 1999-09-15 Chiron Spa Antigenic peptides
CN1416352B (zh) 2000-01-17 2011-05-25 启龙股份公司 含有脑膜炎奈瑟球菌b血清群外膜蛋白质的外膜囊(omv)疫苗
WO2001064863A2 (en) * 2000-02-28 2001-09-07 Stratagene A screening system to identify polynucleotides encoding cleavable n-terminal signal sequences
EP1328543B1 (en) 2000-10-27 2009-08-12 Novartis Vaccines and Diagnostics S.r.l. Nucleic acids and proteins from streptococcus groups a & b
GB0107658D0 (en) 2001-03-27 2001-05-16 Chiron Spa Streptococcus pneumoniae
GB0107661D0 (en) 2001-03-27 2001-05-16 Chiron Spa Staphylococcus aureus
EP1417347A4 (en) * 2001-05-02 2005-06-22 Univ South Florida VECTOR SYSTEM FOR THE SELECTION OF SEPARATED PROTEINS AND MEMBRANE-BONDED PROTEINS CODING GENES
ES2386386T3 (es) 2001-12-12 2012-08-20 Novartis Vaccines And Diagnostics S.R.L. Inmunización contra Chlamydia trachomatis
ATE466875T1 (de) 2002-11-15 2010-05-15 Novartis Vaccines & Diagnostic Unerwartete oberflächenproteine in neisseria meningitidis
GB0308198D0 (en) 2003-04-09 2003-05-14 Chiron Srl ADP-ribosylating bacterial toxin
DK1713930T3 (da) 2004-01-17 2011-01-17 Korea Res Inst Of Bioscience Hurtig screeningsmetode for translationsfusionspartnere til fremstilling af rekombinante proteiner og translationsfusionspartnere screenet derfra
KR20070009269A (ko) * 2005-07-15 2007-01-18 한국생명공학연구원 재조합단백질 생산용 단백질융합인자 라이브러리 및이로부터 획득된 단백질융합인자
US20110206692A1 (en) 2006-06-09 2011-08-25 Novartis Ag Conformers of bacterial adhesins
JP2009017840A (ja) * 2007-07-13 2009-01-29 Japan Agengy For Marine-Earth Science & Technology 外来遺伝子を細胞に安定に保持する方法
KR101431091B1 (ko) * 2008-12-04 2014-08-26 한국생명공학연구원 고분비성 단백질의 스크리닝 및 재조합 단백질의 생산을 위한 융합 파트너로서 그의 용도
US8986956B2 (en) 2010-11-04 2015-03-24 Korea Research Institute Of Bioscience And Biotechnology Method for producing human epidermal growth factor in large volume from yeast

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4711843A (en) * 1980-12-31 1987-12-08 Cetus Corporation Method and vector organism for controlled accumulation of cloned heterologous gene products in Bacillus subtilis
US4585739A (en) * 1983-03-07 1986-04-29 E. I. Du Pont De Nemours And Company Plasmid for foreign gene expression in B. subtilis
US4663280A (en) * 1983-05-19 1987-05-05 Public Health Research Institute Of The City Of New York Expression and secretion vectors and method of constructing vectors
JPS60126087A (ja) * 1983-12-12 1985-07-05 Agency Of Ind Science & Technol プロモ−タ・クロ−ニング用プラスミドｐＴＰ１８−２，ｐＴＰ１８−４及びｐＴＰ１８−６
NL8400687A (nl) * 1984-03-02 1985-10-01 Unilever Nv Recombinant plasmiden; recombinant plasmiden bevattende bacterien; werkwijze voor het bereiden of vervaardigen van een zuivelproduct; werkwijze voor het bereiden van een eiwit.
US4769327A (en) * 1985-03-29 1988-09-06 Biotechnica International, Inc. Secretion vector
JPS61271988A (ja) * 1985-05-27 1986-12-02 Agency Of Ind Science & Technol プラスミド

Also Published As

Publication number Publication date
DE3750378T2 (de) 1995-01-19
FI871919A (fi) 1987-11-03
EP0244042B1 (en) 1994-08-17
ATE110111T1 (de) 1994-09-15
DK221087D0 (da) 1987-04-30
FI871919A0 (fi) 1987-04-29
DK221087A (da) 1987-11-03
EP0244042A1 (en) 1987-11-04
IE64916B1 (en) 1995-09-20
DE3750378D1 (de) 1994-09-22
IE871156L (en) 1987-11-02
PT84801B (pt) 1989-12-29
PT84801A (en) 1987-05-01
US5037760A (en) 1991-08-06
JPS6339586A (ja) 1988-02-20

Similar Documents

Publication Publication Date Title
ES2061482T3 (es) Metodo para la identificacion de secuencias de señal secretora para sintesis de proteina extracelular en bacillus.
MX428A (es) Region regulatoria para expresion de gen heterologa en levadura.
DK82293A (da) Normalt sporulerende bacillus uden evne til at udskille subtilisin eller neutral protease, kulturer deraf og fremgangsmaade til fremstilling af heterologe proteiner dermed
AR000610A1 (es) Un método para la expresión de un polipéptido recombinante por una célula huésped
DE69324359D1 (de) Verbessertes verfahren zur herstellung von endotoxinbindungsproteinen
DE69132530D1 (de) Heparin bindendes mitogen mit homologie zum epidermis wachstumsfaktor (egf)
AR006188A1 (es) "composicion farmaceutica para inhibir la actividad de serinproteasa, metodo in vitro para inhibir la actividad de serinproteasa y metodo para preparar dicha composicion".
DE69329202D1 (de) Methoden der erhöhung der sekretion von überexpremierten proteinen
DE122006000021I1 (de) Dns kodierend für einen für epithelzellen spezifischen Wachstumsfaktor
ATE386126T1 (de) Verbesserte expressionsvektoren
EA200100419A1 (ru) Трансформационная система в области грибов гифомицетов-хозяев
DK496185D0 (da) Autonome replikationssekvenser for gaerstammer af slaegten pichia
FI941048A0 (fi) Geloniinipolypeptidin koodaavia DNA-sekvenssejä
MX9203519A (es) Metodo de recombinacion para la produccion de inhibidores de proteasa de serina y secuencias de adn utiles para los mismos.
ES8800691A1 (es) Un metodo para purificar la proteina inhibidora de fosfolipasa humana (hpip)
NZ307444A (en) Polyphenol oxidase genes from lettuce and banana
ES2065341T3 (es) Vectores y compuestos para la expresion de formas zimogenas de proteina c humana.
ES2134760T3 (es) Polipeptido saf, gen, promotor y uso para la expresion en streptomyces.
ATE88499T1 (de) Expressionsvektoren zur proteinsynthese sowie plasmidreplikons und sequenzkassetten, die zur herstellung solcher vektoren geeignet sind.
ATE302214T1 (de) Enamel-matrix bezogene polypeptide
ES2107997T3 (es) Procedimiento de uso de vectores de expresion eucarioticos que comprenden el potenciador del virus bk.
ATE292683T1 (de) Erhöhung der proteinproduktion in gram-positiven mikroorganismen
SV1999000069A (es) Angiopoyetina relacionada con secuencia del gen 3 en la cicatrizacion de la cara ref. x-12261
DK577283D0 (da) Gen kodet for interleukin-2-polypeptid,rekombinant dna baerende dette gen,levende cellelinie indeholdende dette rekombinante dna og fremgangsmaade til fremstilling af interleukin-2 ved anvendelse af denne cellelinie
ES2060385T3 (es) Nuevos vectores de clonacion y/o de expresion, procedimiento de preparacion y su utilizacion.

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 244042

Country of ref document: ES