DE69329202D1 - Methoden der erhöhung der sekretion von überexpremierten proteinen - Google Patents

Methoden der erhöhung der sekretion von überexpremierten proteinen

Info

Publication number
DE69329202D1
DE69329202D1 DE69329202T DE69329202T DE69329202D1 DE 69329202 D1 DE69329202 D1 DE 69329202D1 DE 69329202 T DE69329202 T DE 69329202T DE 69329202 T DE69329202 T DE 69329202T DE 69329202 D1 DE69329202 D1 DE 69329202D1
Authority
DE
Germany
Prior art keywords
methods
secretion
increasing
overexpressed proteins
host cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69329202T
Other languages
English (en)
Other versions
DE69329202T2 (de
Inventor
Dane Wittrup
Skaja Robinson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Research Corp Technologies Inc
Original Assignee
Research Corp Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Research Corp Technologies Inc filed Critical Research Corp Technologies Inc
Application granted granted Critical
Publication of DE69329202D1 publication Critical patent/DE69329202D1/de
Publication of DE69329202T2 publication Critical patent/DE69329202T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
DE69329202T 1992-10-02 1993-10-04 Methoden der erhöhung der sekretion von überexpremierten proteinen Expired - Fee Related DE69329202T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US95669992A 1992-10-02 1992-10-02
US8999793A 1993-07-06 1993-07-06
PCT/US1993/009426 WO1994008012A1 (en) 1992-10-02 1993-10-04 Methods for increasing secretion of overexpressed proteins

Publications (2)

Publication Number Publication Date
DE69329202D1 true DE69329202D1 (de) 2000-09-14
DE69329202T2 DE69329202T2 (de) 2001-03-29

Family

ID=26781150

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69329202T Expired - Fee Related DE69329202T2 (de) 1992-10-02 1993-10-04 Methoden der erhöhung der sekretion von überexpremierten proteinen

Country Status (6)

Country Link
US (1) US5773245A (de)
EP (1) EP0663010B1 (de)
AT (1) ATE195347T1 (de)
DE (1) DE69329202T2 (de)
DK (1) DK0663010T3 (de)
WO (1) WO1994008012A1 (de)

Families Citing this family (50)

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US20030036092A1 (en) * 1991-11-15 2003-02-20 Board Of Regents, The University Of Texas System Directed evolution of enzymes and antibodies
US5866344A (en) * 1991-11-15 1999-02-02 Board Of Regents, The University Of Texas System Antibody selection methods using cell surface expressed libraries
US5639635A (en) * 1994-11-03 1997-06-17 Genentech, Inc. Process for bacterial production of polypeptides
US5789199A (en) * 1994-11-03 1998-08-04 Genentech, Inc. Process for bacterial production of polypeptides
US5688651A (en) * 1994-12-16 1997-11-18 Ramot University Authority For Applied Research And Development Ltd. Prevention of protein aggregation
WO1997038123A1 (en) * 1996-04-05 1997-10-16 Board Of Regents, The University Of Texas System Methods for producing soluble, biologically-active disulfide bond-containing eukaryotic proteins in bacterial cells
FR2755143B1 (fr) * 1996-10-25 1998-11-27 Rhone Poulenc Nutrition Animal Procede de preparation de l'acide 2-hydroxy-4-methylthio-butyrique par utilisation d'une nitrilase
US5919999A (en) 1996-11-14 1999-07-06 Queen's University At Kingston Enhanced transport with a plastid membrane transport protein
GB9703406D0 (en) 1997-02-19 1997-04-09 Chiron Spa Expression of heterologous proteins
US6083715A (en) * 1997-06-09 2000-07-04 Board Of Regents, The University Of Texas System Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells
US6521421B1 (en) 1997-07-16 2003-02-18 Genencor International, Inc. Expression vectors encoding Bacillus subtilis disulfide bond isomerase and methods of secreting proteins in gram-positive microorganisms using the same
EP0927757A1 (de) * 1997-12-03 1999-07-07 Leadd B.V. Verfahren und Mitteln um Apoptose zu induzieren durch Interferenz mit Bip-ähnliche Proteine
US20010027182A1 (en) * 1998-05-29 2001-10-04 Huda Y. Zoghbi Chaperone suppression of ataxin-1 aggregation and altered subcellular proteasome localization imply protein misfilind in sca1
GB9822115D0 (en) 1998-10-09 1998-12-02 King S College London Treatment of inflammatory disease
GB9827480D0 (en) * 1998-12-15 1999-02-10 Univ York Plant-pathogen resistance
FI109361B (fi) * 1999-05-04 2002-07-15 Korpela Timo Mikrobinen proteiinin ilmentämisjärjestelmä
US6495360B1 (en) * 1999-05-28 2002-12-17 Photogen, Inc. Method for enhanced protein stabilization and for production of cell lines useful for production of such stabilized proteins
US6677139B1 (en) 1999-12-23 2004-01-13 Genecor International, Inc. Methods for production of proteins in host cells
US6808900B2 (en) * 2000-06-15 2004-10-26 Manitoba, University Of Cryptosporidium parvum antigens, antibodies thereto and diagnostic and therapeutic compositions thereof
CA2449747C (en) 2001-06-22 2010-04-13 F. Hoffmann-La Roche Ag Use of fkbp chaperones as expression tool
US7094884B2 (en) 2001-06-22 2006-08-22 Roche Diagnostics Corporation Soluble complexes of amylod β peptide and peptidyl prolyl isomerase chaperone and methods of making and using them
US7094757B2 (en) 2001-06-22 2006-08-22 Roche Diagnostics Corporation Complexes comprising a prion protein and a peptidyl prolyl isomerase chaperone, and method for producing and using them
US6962982B2 (en) 2001-06-22 2005-11-08 Roche Diagnostics Corporation Soluble complexes of target proteins and peptidyl prolyl isomerase chaperones and methods of making and using them
KR100468482B1 (ko) * 2002-04-11 2005-01-27 양문식 효모 발현 시스템을 이용한 인터루킨-18 단백질의생산방법
US20040157289A1 (en) * 2002-09-06 2004-08-12 Salerno John C. Protein expression system
US20040203132A1 (en) 2003-02-28 2004-10-14 Cognetix, Inc. Conus protein disulfide isomerase
US7244616B2 (en) 2003-06-27 2007-07-17 Bayer Pharmaceuticals Corporation Use of molecular chaperones for the enhanced production of secreted, recombinant proteins in mammalian cells
GB0329681D0 (en) * 2003-12-23 2004-01-28 Delta Biotechnology Ltd Gene expression technique
GB0329722D0 (en) * 2003-12-23 2004-01-28 Delta Biotechnology Ltd Modified plasmid and use thereof
DK2339015T3 (en) 2004-03-31 2018-08-06 Janssen Biotech Inc Methods for changing protein production rates
EA011606B1 (ru) * 2004-07-14 2009-04-28 Эдуард Борисович Татулов Способы получения и применения рекомбинантного альфа-фетопротеина
EP1831375B1 (de) 2004-12-23 2014-07-16 Novozymes Biopharma DK A/S Genexpressionsmethode
DE102005027557B4 (de) * 2005-06-14 2012-12-13 RUHR-UNIVERSITäT BOCHUM Koexpression von Chaperonen der Hsp70-Familie zur Verstärkung der Expressionen von G-Protein gekoppelten Rezeptoren
GB0512707D0 (en) * 2005-06-22 2005-07-27 Delta Biotechnology Ltd Gene expression technique
US8034607B2 (en) * 2007-09-12 2011-10-11 Wisconsin Alumni Research Foundation Methods of enhanced heterologous protein secretion
AU2008318644B2 (en) * 2007-11-01 2013-05-02 Danisco Us Inc. Signal sequences and co-expressed chaperones for improving protein production in a host cell
EP2245151B1 (de) * 2008-02-20 2014-12-24 GlycoFi, Inc. Vektoren und hefestämme für die proteinproduktion
FR2933773B1 (fr) * 2008-07-10 2013-02-15 Biomerieux Sa Procede de dosage de la proteine disulfide isomerase pour le diagnostic in vitro du cancer colorectal
EP2258854A1 (de) 2009-05-20 2010-12-08 FH Campus Wien Eukaryotische Wirtszelle mit einem Ausdrucksverstärker
WO2010139048A1 (en) 2009-06-01 2010-12-09 Kenneth James Friel Human milk peptides
WO2011154908A1 (en) * 2010-06-08 2011-12-15 National University Of Ireland, Galway Manipulation of hsp70 and ire1alpha protein interactions
EP2565262A1 (de) 2011-08-31 2013-03-06 VTU Holding GmbH Protein-Expression
KR102373954B1 (ko) 2014-04-17 2022-03-14 베링거 인겔하임 에르체파우 게엠베하 운트 코 카게 보조 단백질을 과발현하도록 조작된 재조합 숙주세포
CA2945877C (en) 2014-04-17 2023-09-05 Boehringer Ingelheim Rcv Gmbh & Co Kg Recombinant host cell for expressing proteins of interest
WO2020069214A2 (en) * 2018-09-26 2020-04-02 Demetrix, Inc. Optimized expression systems for producing cannabinoid synthase polypeptides, cannabinoids, and cannabinoid derivatives
CA3117992A1 (en) 2018-11-08 2020-05-14 Sutro Biopharma, Inc. E coli strains having an oxidative cytoplasm
US20210340586A1 (en) 2020-04-30 2021-11-04 Sutro Biopharma, Inc. Methods of Producing Full-Length Antibodies Using E. coli
CN116888142A (zh) * 2021-01-26 2023-10-13 丹斯塔发酵股份公司 具有提高的生长速率的重组酵母宿主细胞
CA3213051A1 (en) * 2021-03-10 2022-09-15 Sumitomo Pharma Co., Ltd. Method for producing cysteine knot protein
JPWO2022191253A1 (de) * 2021-03-10 2022-09-15

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0509841A3 (en) * 1991-04-18 1993-08-18 Tonen Corporation Co-expression system of protein disulfide isomerase gene and useful polypeptide gene and process for producing the polypeptide using its system
NL9102009A (nl) * 1991-11-29 1993-06-16 Stichting Katholieke Univ Produktie van bioactieve peptiden met recombinante cellen.

Also Published As

Publication number Publication date
WO1994008012A1 (en) 1994-04-14
ATE195347T1 (de) 2000-08-15
EP0663010B1 (de) 2000-08-09
DE69329202T2 (de) 2001-03-29
EP0663010A1 (de) 1995-07-19
DK0663010T3 (da) 2000-11-13
US5773245A (en) 1998-06-30

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Legal Events

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8339 Ceased/non-payment of the annual fee