ATE195347T1 - Methoden der erhöhung der sekretion von überexpremierten proteinen - Google Patents

Methoden der erhöhung der sekretion von überexpremierten proteinen

Info

Publication number
ATE195347T1
ATE195347T1 AT93922826T AT93922826T ATE195347T1 AT E195347 T1 ATE195347 T1 AT E195347T1 AT 93922826 T AT93922826 T AT 93922826T AT 93922826 T AT93922826 T AT 93922826T AT E195347 T1 ATE195347 T1 AT E195347T1
Authority
AT
Austria
Prior art keywords
methods
increase secretion
overexpressed proteins
host cell
overexpressed
Prior art date
Application number
AT93922826T
Other languages
English (en)
Inventor
Karl Dane Wittrup
Anne Skaja Robinson
Original Assignee
Res Corp Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Res Corp Technologies Inc filed Critical Res Corp Technologies Inc
Application granted granted Critical
Publication of ATE195347T1 publication Critical patent/ATE195347T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Mycology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
AT93922826T 1992-10-02 1993-10-04 Methoden der erhöhung der sekretion von überexpremierten proteinen ATE195347T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US95669992A 1992-10-02 1992-10-02
US8999793A 1993-07-06 1993-07-06
PCT/US1993/009426 WO1994008012A1 (en) 1992-10-02 1993-10-04 Methods for increasing secretion of overexpressed proteins

Publications (1)

Publication Number Publication Date
ATE195347T1 true ATE195347T1 (de) 2000-08-15

Family

ID=26781150

Family Applications (1)

Application Number Title Priority Date Filing Date
AT93922826T ATE195347T1 (de) 1992-10-02 1993-10-04 Methoden der erhöhung der sekretion von überexpremierten proteinen

Country Status (6)

Country Link
US (1) US5773245A (de)
EP (1) EP0663010B1 (de)
AT (1) ATE195347T1 (de)
DE (1) DE69329202T2 (de)
DK (1) DK0663010T3 (de)
WO (1) WO1994008012A1 (de)

Families Citing this family (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030036092A1 (en) * 1991-11-15 2003-02-20 Board Of Regents, The University Of Texas System Directed evolution of enzymes and antibodies
US5866344A (en) * 1991-11-15 1999-02-02 Board Of Regents, The University Of Texas System Antibody selection methods using cell surface expressed libraries
US5789199A (en) * 1994-11-03 1998-08-04 Genentech, Inc. Process for bacterial production of polypeptides
US5639635A (en) * 1994-11-03 1997-06-17 Genentech, Inc. Process for bacterial production of polypeptides
US5688651A (en) * 1994-12-16 1997-11-18 Ramot University Authority For Applied Research And Development Ltd. Prevention of protein aggregation
KR970010968A (ko) * 1995-08-24 1997-03-27 윤원영 오리 배 세포를 이용한 에리스로포이틴의 발현 시스템
US6027888A (en) * 1996-04-05 2000-02-22 Board Of Regents, The University Of Texas System Methods for producing soluble, biologically-active disulfide-bond containing eukaryotic proteins in bacterial cells
FR2755143B1 (fr) * 1996-10-25 1998-11-27 Rhone Poulenc Nutrition Animal Procede de preparation de l'acide 2-hydroxy-4-methylthio-butyrique par utilisation d'une nitrilase
US5919999A (en) 1996-11-14 1999-07-06 Queen's University At Kingston Enhanced transport with a plastid membrane transport protein
GB9703406D0 (en) * 1997-02-19 1997-04-09 Chiron Spa Expression of heterologous proteins
US6083715A (en) * 1997-06-09 2000-07-04 Board Of Regents, The University Of Texas System Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells
US6521421B1 (en) 1997-07-16 2003-02-18 Genencor International, Inc. Expression vectors encoding Bacillus subtilis disulfide bond isomerase and methods of secreting proteins in gram-positive microorganisms using the same
EP0927757A1 (de) * 1997-12-03 1999-07-07 Leadd B.V. Verfahren und Mitteln um Apoptose zu induzieren durch Interferenz mit Bip-ähnliche Proteine
US20010027182A1 (en) * 1998-05-29 2001-10-04 Huda Y. Zoghbi Chaperone suppression of ataxin-1 aggregation and altered subcellular proteasome localization imply protein misfilind in sca1
GB9822115D0 (en) 1998-10-09 1998-12-02 King S College London Treatment of inflammatory disease
GB9827480D0 (en) * 1998-12-15 1999-02-10 Univ York Plant-pathogen resistance
FI109361B (fi) 1999-05-04 2002-07-15 Korpela Timo Mikrobinen proteiinin ilmentämisjärjestelmä
US6495360B1 (en) * 1999-05-28 2002-12-17 Photogen, Inc. Method for enhanced protein stabilization and for production of cell lines useful for production of such stabilized proteins
US6677139B1 (en) 1999-12-23 2004-01-13 Genecor International, Inc. Methods for production of proteins in host cells
US6808900B2 (en) 2000-06-15 2004-10-26 Manitoba, University Of Cryptosporidium parvum antigens, antibodies thereto and diagnostic and therapeutic compositions thereof
US6962982B2 (en) 2001-06-22 2005-11-08 Roche Diagnostics Corporation Soluble complexes of target proteins and peptidyl prolyl isomerase chaperones and methods of making and using them
KR100702373B1 (ko) 2001-06-22 2007-04-02 에프. 호프만-라 로슈 아게 레트로바이러스 표면 당단백질을 함유하는 가용성 복합체
US7094884B2 (en) 2001-06-22 2006-08-22 Roche Diagnostics Corporation Soluble complexes of amylod β peptide and peptidyl prolyl isomerase chaperone and methods of making and using them
US7094757B2 (en) 2001-06-22 2006-08-22 Roche Diagnostics Corporation Complexes comprising a prion protein and a peptidyl prolyl isomerase chaperone, and method for producing and using them
KR100468482B1 (ko) * 2002-04-11 2005-01-27 양문식 효모 발현 시스템을 이용한 인터루킨-18 단백질의생산방법
US20040157289A1 (en) * 2002-09-06 2004-08-12 Salerno John C. Protein expression system
US20040203132A1 (en) 2003-02-28 2004-10-14 Cognetix, Inc. Conus protein disulfide isomerase
US7244616B2 (en) 2003-06-27 2007-07-17 Bayer Pharmaceuticals Corporation Use of molecular chaperones for the enhanced production of secreted, recombinant proteins in mammalian cells
GB0329722D0 (en) * 2003-12-23 2004-01-28 Delta Biotechnology Ltd Modified plasmid and use thereof
GB0329681D0 (en) * 2003-12-23 2004-01-28 Delta Biotechnology Ltd Gene expression technique
KR101145308B1 (ko) 2004-03-31 2012-05-14 얀센 바이오테크 인코포레이티드 단백질 생산 속도를 변경하는 방법
EA011606B1 (ru) * 2004-07-14 2009-04-28 Эдуард Борисович Татулов Способы получения и применения рекомбинантного альфа-фетопротеина
JP5631533B2 (ja) 2004-12-23 2014-11-26 ノボザイムス バイオファーマ デーコー アクティーゼルスカブ 遺伝子発現技術
DE102005027557B4 (de) * 2005-06-14 2012-12-13 RUHR-UNIVERSITäT BOCHUM Koexpression von Chaperonen der Hsp70-Familie zur Verstärkung der Expressionen von G-Protein gekoppelten Rezeptoren
GB0512707D0 (en) * 2005-06-22 2005-07-27 Delta Biotechnology Ltd Gene expression technique
US8034607B2 (en) * 2007-09-12 2011-10-11 Wisconsin Alumni Research Foundation Methods of enhanced heterologous protein secretion
MX2010004325A (es) * 2007-11-01 2010-05-20 Danisco Us Inc Secuencias de señal y chaperones co-expresados para mejorar produccion de proteinas en una celula hospedera.
CN101945998B (zh) * 2008-02-20 2013-09-18 格利科菲公司 用于蛋白生产的载体和酵母菌株
FR2933773B1 (fr) * 2008-07-10 2013-02-15 Biomerieux Sa Procede de dosage de la proteine disulfide isomerase pour le diagnostic in vitro du cancer colorectal
EP2258854A1 (de) 2009-05-20 2010-12-08 FH Campus Wien Eukaryotische Wirtszelle mit einem Ausdrucksverstärker
EP2448958A4 (de) 2009-06-01 2013-11-06 Kenneth James Friel Menschliche milchpeptide
EP2580232A1 (de) * 2010-06-08 2013-04-17 National University of Ireland, Galway Manipulation von hsp70- und ire1alpha-proteininteraktionen
EP2565262A1 (de) * 2011-08-31 2013-03-06 VTU Holding GmbH Protein-Expression
CA2948369C (en) 2014-04-17 2023-09-26 Boehringer Ingelheim Rcv Gmbh & Co Kg Recombinant host cell engineered to overexpress helper proteins
SG11201608080RA (en) 2014-04-17 2016-10-28 Boehringer Ingelheim Rcv Gmbh Recombinant host cell for expressing proteins of interest
WO2020069214A2 (en) * 2018-09-26 2020-04-02 Demetrix, Inc. Optimized expression systems for producing cannabinoid synthase polypeptides, cannabinoids, and cannabinoid derivatives
EP3877552B1 (de) 2018-11-08 2023-02-22 Sutro Biopharma, Inc. E. coli-stämme mit einem oxidativen zytoplasma
US12351850B2 (en) 2020-04-30 2025-07-08 Sutro Biopharma, Inc. Methods of producing full-length antibodies using E. coli
CN116888142A (zh) * 2021-01-26 2023-10-13 丹斯塔发酵股份公司 具有提高的生长速率的重组酵母宿主细胞
CA3213053A1 (en) * 2021-03-10 2022-09-15 Sumitomo Pharma Co., Ltd. Method for producing fusion protein
CA3213051A1 (en) * 2021-03-10 2022-09-15 Sumitomo Pharma Co., Ltd. Method for producing cysteine knot protein
CN115948448A (zh) * 2022-03-07 2023-04-11 北京迈益合生物医药科技有限公司 一种在毕赤酵母中高效表达非特异性核酸内切酶的方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0509841A3 (en) * 1991-04-18 1993-08-18 Tonen Corporation Co-expression system of protein disulfide isomerase gene and useful polypeptide gene and process for producing the polypeptide using its system
NL9102009A (nl) * 1991-11-29 1993-06-16 Stichting Katholieke Univ Produktie van bioactieve peptiden met recombinante cellen.

Also Published As

Publication number Publication date
WO1994008012A1 (en) 1994-04-14
EP0663010B1 (de) 2000-08-09
DE69329202T2 (de) 2001-03-29
US5773245A (en) 1998-06-30
EP0663010A1 (de) 1995-07-19
DE69329202D1 (de) 2000-09-14
DK0663010T3 (da) 2000-11-13

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