AU6045196A - Secretion leader trap cloning method - Google Patents

Secretion leader trap cloning method

Info

Publication number
AU6045196A
AU6045196A AU60451/96A AU6045196A AU6045196A AU 6045196 A AU6045196 A AU 6045196A AU 60451/96 A AU60451/96 A AU 60451/96A AU 6045196 A AU6045196 A AU 6045196A AU 6045196 A AU6045196 A AU 6045196A
Authority
AU
Australia
Prior art keywords
cloning method
secretion leader
trap cloning
trap
leader
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU60451/96A
Inventor
Si Lok
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zymogenetics Inc
Original Assignee
Zymogenetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zymogenetics Inc filed Critical Zymogenetics Inc
Publication of AU6045196A publication Critical patent/AU6045196A/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/32—Fusion polypeptide fusions with soluble part of a cell surface receptor, "decoy receptors"
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU60451/96A 1995-06-07 1996-06-04 Secretion leader trap cloning method Abandoned AU6045196A (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US48349395A 1995-06-07 1995-06-07
US47280695A 1995-06-07 1995-06-07
US483493 1995-06-07
US472806 1995-06-07
PCT/US1996/008936 WO1996040904A1 (en) 1995-06-07 1996-06-04 Secretion leader trap cloning method

Publications (1)

Publication Number Publication Date
AU6045196A true AU6045196A (en) 1996-12-30

Family

ID=27043933

Family Applications (1)

Application Number Title Priority Date Filing Date
AU60451/96A Abandoned AU6045196A (en) 1995-06-07 1996-06-04 Secretion leader trap cloning method

Country Status (5)

Country Link
EP (1) EP0832209A1 (en)
JP (1) JPH11506939A (en)
AU (1) AU6045196A (en)
CA (1) CA2223155C (en)
WO (1) WO1996040904A1 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5536637A (en) * 1993-04-07 1996-07-16 Genetics Institute, Inc. Method of screening for cDNA encoding novel secreted mammalian proteins in yeast
GB9701492D0 (en) * 1997-01-24 1997-03-12 Univ Edinburgh Transfection of embryonic stem cells
WO1999005256A2 (en) * 1997-07-24 1999-02-04 President And Fellows Of Harvard College Method for cloning secreted proteins
CA2311678C (en) 1997-11-26 2006-09-19 Chugai Seiyaku Kabushiki Kaisha Signal sequence trapping method
US6150098A (en) * 1998-02-20 2000-11-21 Amgen Inc. Methods for identifying novel secreted mammalian polypeptides
US6103472A (en) * 1998-02-20 2000-08-15 Amgen Inc. Methods and compositions for identifying novel secreted mammalian polypeptides in yeast
AU3193399A (en) 1998-03-23 1999-10-18 Genentech Inc. Method of selection for genes encoding secreted and transmembrane proteins
EP1081224B1 (en) 1998-05-20 2006-06-07 Chugai Seiyaku Kabushiki Kaisha Novel method for cloning a gene
WO2001009310A1 (en) * 1999-07-29 2001-02-08 Berlex Laboratories, Inc. 5'ENRICHED cDNA LIBRARIES AND A YEAST SIGNAL TRAP
WO2002088325A2 (en) 2001-05-02 2002-11-07 University Of South Florida Vector system for selection of genes encoding secreted proteins and membrane-bound proteins

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3750378T2 (en) * 1986-05-02 1995-01-19 Gist Brocades Nv Secretion signal selection vectors for extracellular protein synthesis in bacilli.
US5817797A (en) * 1988-06-01 1998-10-06 The United States Of America As Represented By The Department Of Health And Human Services Sequencing DNA; a modification of the polymerase chain reaction
DE3901681A1 (en) * 1989-01-21 1990-07-26 Behringwerke Ag SIGNAL PEPTIDE FOR THE SECRETION OF PEPTIDES IN ESCHERICHIA COLI, METHOD FOR ITS EFFECT AND ITS USE
GB8926269D0 (en) * 1989-11-21 1990-01-10 Dynal As Plasmid
JP2879303B2 (en) * 1993-01-14 1999-04-05 佑 本庶 Method for preparing cDNA library, novel polypeptide and DNA encoding the same
DE4332463A1 (en) * 1993-09-24 1995-03-30 Boehringer Mannheim Gmbh Process for the specific cloning of nucleic acids

Also Published As

Publication number Publication date
WO1996040904A1 (en) 1996-12-19
JPH11506939A (en) 1999-06-22
CA2223155C (en) 2003-08-12
CA2223155A1 (en) 1996-12-19
EP0832209A1 (en) 1998-04-01

Similar Documents

Publication Publication Date Title
AU3677697A (en) Automatic auction method
AUPN453895A0 (en) Spray method
AU5262996A (en) Telecommunications reward method
FI98591B (en) Video subtitle method
AU3768095A (en) Herbicidal composition and method
AU1772797A (en) Lens injection-compression-molding method
AU6045196A (en) Secretion leader trap cloning method
AUPM970294A0 (en) An optical control method
ZA965422B (en) Method for controlling insects
AU2183997A (en) New method
AU3704297A (en) Method
AU679363B2 (en) Channel sounding method
GB2314348B (en) Thin film formation method
AU4682796A (en) Automatically downfolding slab
AU7537596A (en) Improved nogging for building purposes
EP0771751A3 (en) Winding apparatus
AU1101797A (en) Method for making fluoroalkanols
AU5271296A (en) Novel method
ZA982347B (en) Method for grafting rootstock using improved grafting film
AU1138397A (en) Novel method
AU1000795A (en) Moquitoe control method
AU3776697A (en) Method
GB2338962B (en) Thin film formation method
AU4456196A (en) Herbicidal method
AU695985B2 (en) Subscriber interface control method